Digital Commons Network^™

Trash Talk: The Effects Of Plastic Pollution On Seabirds In Narragansett Bay, Erin A. O'Neill

Pell Scholars and Senior Theses

Plastic pollution in the ocean is a global concern with more than 8 million tons of plastic dumped into our oceans every year. This policy paper assesses plastic pollution in Narragansett Bay and the negative implications it holds on local seabird populations. Also, essential background information on plastic production and throwaway culture is provided. Moreover, the biological significance of seabirds is described, highlighting the vital role such populations play in local ecosystems such as Narragansett Bay. This paper contributes research to the global issue of plastic pollution by observing declining native wildlife life populations, such as seabirds, on a local …

Hispanic Culture And Machismo: How Hispanics Interpret Transgender And Gender Non-Conforming Identities, Karen R. Rodriguez

Theses and Dissertations

This study examines dynamics linked to normative gender expectations and gender identities within Hispanic culture. Little is known in this regard concerning how Hispanics perceive and interpret transgender and gender non-conforming identities. The analysis provides qualitative assessment using 35 in-depth face-to-face interviews and examines the following research questions: 1) how do life experiences such as marriage, parenthood, and work force participation among Hispanics shape their formation of gender expectations? 2) Among Hispanics, how do class, race/ethnicity, and education shape the construction of gender ideologies.

The present study, in turn adds to the literature by examining gender norm constructs among Mexican-origin …

Varying Dataset Resolution Alters Predictive Accuracy Of Spatially Explicit Ensemble Models For Avian Species Distribution, Claire M. Curry, Jeremy D. Ross, Andrea Contina, Eli S. Bridge

School of Integrative Biological & Chemical Sciences Faculty Publications

Species distribution models can be made more accurate by use of new “Spatiotemporal Exploratory Models” (STEMs), a type of spatially explicit ensemble model (SEEM) developed at the continental scale that averages regional models pixel by pixel. Although SEEMs can generate more accurate predictions of species distributions, they are computationally expensive. We compared the accuracies of each model for 11 grassland bird species and examined whether they improve accuracy at a statewide scale for fine and coarse predictor resolutions. We used a combination of survey data and citizen science data for 11 grassland bird species in Oklahoma to test a spatially …

Is Tpmt Testing A Predictor Of Duration Of Use Or Long-Term Benefit Of Thiopurine Agents?, Shatoya R. Wilson

Masters Theses

Background: Inflammatory Bowel Disease (IBD) affects as many as 1.3 million Americans and includes both ulcerative colitis (UC) and Crohn’s disease (CD). Recent practices have moved towards using immunomodulators early in the course of disease to prevent disease progression. Patients have shown improved outcomes on immunomodulators compared to aminosalicylates, and reduced risk for tachyphylaxis compared to corticosteroid treatment. Thiopurine agents such as azathioprine and 6-mercaptopurine are immunomodulators often used to treat IBD. Unfortunately, many patients experience cytotoxicity from thiopurine use. Recent studies have shown that patients deficient in thiopurine-S-methyltransferase (TMPT), produce an overabundance of the therapeutic end product, 6-thioguanine nucleotide …

Evaluation Of Bioactive Compounds On Different Extracts Of Linum Usitatissimum And Its Antimicrobial Properties Against Selected Oral Pathogens, Ummu A. Fadzir, Khairani I. Mokhtar, Basma E. Mustafa, Deny S. Darnis

Makara Journal of Health Research

Background: Antibacterial agents from natural sources have been suggested as alternatives for treating infectious diseases due to their low side effects. Flaxseed (Linum usitatissimum) exhibits some antibacterial properties, but its effects against oral pathogens are poorly understood. This study investigated flaxseed extracts' antibacterial effects against such pathogens. Methods: Non-polar and polar extracts of flaxseed, with n-hexane, dichloromethane (DCM) and methanol (MeOH), were prepared by sequential Soxhlet extraction. All extracts were qualitatively screened through gas chromatography-mass spectrometry to detect bioactive compounds. Antibacterial activities of flaxseed extracts with different volumes per disc (Oxoid, Badhoevedorp, Netherlands) (5, 7, 10, 12 and 15 µL/disc) …

A Systematic Review Of The Association Between Intrapersonal Factors And Smoking Cessation In Adolescents In The United States, Amanita K. Hana, Sung-Il Cho, Eunsil Cheon, Suhyun Kwon, Rajapakshe Thishani

Makara Journal of Health Research

Background: Premature death in developed countries, and smoking by adolescents is a serious health issue in the US. There are several aspects that influence smoking cessation in adolescents, one of which comprises intrapersonal factors. The aim of this systematic review was therefore to identify and categorize specific intrapersonal factors that affect the smoking cessation of adolescents in the United States. Methods: Through a search on PubMed, identified quantitative research studies on the intrapersonal factors affecting the smoking cessation of adolescents in the US. The search results imported into Endnote X9. The intrapersonal factors examined into 4 classifications: nicotine dependence, stress, …

Effects Of Physical Exercise On Indicators Of Inflammation Risk Of The Gaster In A Male Wistar Rat Aging Model Created With D-Galactose Induction, Dian Murwaningsih, Andreanyta Meliala, Zaenal M. Sofro

Makara Journal of Health Research

Background: Physical exercise is a non-pharmacological treatment for various diseases. Aging is associated with deteriorating physiological function, and elderly individuals generally have inflammation or infection in the digestive tract. This study aimed to examine the effects of mild and moderate physical exercise intensities on the indicators of inflammation risk of the gaster in a male Wistar rat aging model created with D-galactose induction. Methods: This experimental research study had a post-test-only group design. The study included 24 3-month-old male Wistar rats weighing 200-300 g. The rats were equally divided into four groups (saline,+light-intensity physical exercise, and D-galactose+moderate-intensity physical exercise)D-galactose, D-galactose. …

Data On Spectrum-Based Fluorescence Resonance Energy Transfer Measurement Of E. Coli Multidrug Transporter Acrb, Yuguang Cai, Thomas E. Wilkop, Yinan Wei

Chemistry Faculty Publications

This paper presented the dataset of correction parameters used in the determination of the energy transfer efficiencies from the spectrum-based fluorescence resonance energy transfer (FRET) measurement in a trimeric membrane protein AcrB. The cyan fluorescent protein (CFP) and yellow fluorescent protein (YPet) were used as the donor and acceptor, respectively. Two AcrB fusion proteins were constructed, AcrB-CFP and AcrB-YPet. The proteins were co-expressed in Escherichia coli cells, and energy transfer efficiency were determined in live cells. To obtain reliable energy transfer data, a complete set of correction parameters need to be first determined to accommodate for factors such as background …

Using Offline Activities To Enhance Online Cybersecurity Education, Sarah Padlipsky

Master's Theses

Since the beginning of the 21st century, the United States has experienced the impact of a technological revolution. One effect of this technological revolution is the creation of entirely new careers related to the field of technology, including cybersecurity. Continued growth in the cybersecurity industry means a greater number of jobs will be created, adding to the existing number of jobs that are challenging an under-educated and under-trained workforce. The goal of this thesis is to increase the effectiveness of cybersecurity education. This thesis studies whether an online course in cybersecurity can be enhanced by offline, in-person activities that mirror …

Labor And Social Identity In Ancient Peru: A Bioarchaeological Perspective, Sarah Katherine Muno

Dissertations

AN ABSTRACT OF THE DISSERTATION OF Sarah K. Muno for the Doctor of Philosophy degree in Anthropology, presented on September 26, 2018 at Southern Illinois University Carbondale. TITLE: LABOR AND SOCIAL IDENTITY IN ANCIENT PERU: A BIOARCHAEOLOGICAL PERSPECTIVE MAJOR PROFESSOR: Dr. Izumi Shimada This dissertation presents a bioarchaeological study of labor and social identity in coastal Peru during the Late Intermediate Period (900 – 1470 CE), using data from contemporaneous Middle Sicán (Sicán Precinct and El Brujo, north coast) and Ychsma (Pachacamac, central coast) mortuary contexts. I combine information about funerary treatment with skeletal evidence of trauma, degenerative joint disease, …

Investigation Of Erg1a Potassium Channel Modulation Of Calpain Activity In C2c12 Myotubes, Clayton Alan Whitmore

Theses

Skeletal muscle atrophy is a result of an imbalance in protein synthesis and protein degradation. It is a part of the normal aging process, but also may occur in response to several stimuli including disease, injury, and disuse. Currently there is no truly effective pharmacological treatment for atrophy. The best treatment is a healthy diet and exercise, however, sick and aging individuals are not always capable of participating in the appropriate activity. Therefore, exploration of the mechanisms contributing to atrophy is essential because this may lead to discovery of an effective therapeutic target. The ether-a-go-go related gene (Erg1a) K+ channel …

Construction Of Efl Teacher Educators’ Knowledge Base In A Teacher Education Program In Nicaragua, Angel María Dávila

Dissertations

AN ABSTRACT OF THE DISSERTATION The purpose of this qualitative phenomenological study was to understand and describe the sources of Nicaraguan EFL teacher educators’ knowledge base, the types of knowledge and skills that constructed their knowledge base, and the relationship of this knowledge base and classroom practices in a teacher education program at a Nicaraguan University. This study presents a literature review on the sources of knowledge and knowledge base of EFL teacher educators in the field of language teacher education. I used a purposeful sampling technique to select both the research site and the six EFL teacher educators who …

Christian Theistic Anthropology, Anthony T. Vento

Doctoral Dissertations and Projects

At the present moment, which is prior to conducting any research, I am of the opinion that anthropology has been influenced by certain schools of thought, and each school’s definition establishes a trajectory which definitively determines the adherent’s worldview. The schools of anthropology which are prevalent in today’s world, in my estimation, are the evolutionist’s perspective, the hedonistic perspective, the rationalistic perspective, the dualistic perspective and the Christian perspective. I will seek to objectively and unbiasedly define each perspective’s understanding of man, then I will compare and contrast the main tenets of each school of thought, and finally, I will …

Breaking The Silence: A Phenomenological Study Of Introverted Undergraduate Students' Experiences In The Active Learning English Classroom, Richard Lee Green

Doctoral Dissertations and Projects

The purpose of this transcendental phenomenological study was to describe the experiences of introverted undergraduate students in the active learning English classroom in a community college setting in Florida. Eysenck’s Personality Theory, which provides knowledge about introverts’ preferred method of learning; and Experiential Learning Theory, which presents pertinent information concerning how individuals learn by experience, guided this study. The research questions were as follows: How do introverted undergraduate students describe their experience in an English course structured as an active learning classroom environment (ALC)? How do participants describe the academic atmosphere of the ALC? How do participants describe the effect …

Standardized High School Assessments As An Indicator Of Academic Preparedness In First-Year University Students, Jessica M. Scott

Doctoral Dissertations and Projects

SAT scores have a role in whether or not high school graduates get accepted into the school of their choice, as well as the program of study. Pennsylvania requires students to participate in Keystone Exams, assessments concentrating on one subject area at a time, given sequentially upon the students’ course completion. Both assessments are used as a projection of how well a student will perform at his or her next level of study. Universities look at SAT scores, ACT scores, high school grade point averages (HSGPA), core subject grades, etc. to evaluate whether or not the student will be admitted …

Impact Of Positive Behavior Interventions And Supports On At-Risk, Black Male Students' Academic Performance, Sandra Adomako Letterlough

Doctoral Dissertations and Projects

Black male students have experienced disproportionate disciplinary actions at higher rates compared to male students of other races, which has an at-risk effect on academic achievement because of lost instructional time, poor student-teacher relationships, and decreased motivation to learn. Implementing Positive Behavior Intervention and Supports (PBIS) could help to reduce the time spent out of class due to a disciplinary issue, thus increasing the opportunity to learn. The purpose of this causal-comparative study was to determine the impact of PBIS on academic achievement and frequency of office disciplinary referrals (ODR) in a PBIS and non-PBIS alternative middle school. The sample …

Genetic Evolution And Prognostic Determinants Of Pancreatic Cancer On Longitudinal Liquid Biopsies, Vincent Bernard

Dissertations and Theses (Open Access)

Pancreatic ductal adenocarcinoma (PDAC) has one of the lowest 5-year survival rates amongst solid tumors. As early detection of PDAC is unusual and typically incidental, most patients present with locally advanced and metastatic disease where effective therapeutic strategies remain a significant unmet need. Specifically, surrogate biomarkers for tumor monitoring of PDAC may lead to improved elucidation of clinical actionability and prognostic potential. On the other hand, tumor tissue is rarely sampled in patients presenting with de novo or recurrent metastatic PDAC, apart from a fine needle aspiration or a core needle biopsy performed for diagnosis. This precludes the opportunity for …

The Role Of Tumor Suppressor Dear1 In The Acquisition Of Mammary Stem/Progenitor Cell Properties, Uyen Le

Dissertations and Theses (Open Access)

Breast cancer is the most commonly diagnosed cancer in women in America. Ductal carcinoma in situ (DCIS), one of the earliest pre-invasive forms of invasive ductal carcinoma (IDC), has a 30-50% risk of progressing to IDC. Understanding the mechanisms regulating progression from DCIS to IDC would help identify biomarkers to stratify patients at higher risk of progression or metastasis. Cumulative literature suggests the earliest phase of dissemination from the primary tumor is driven by the epithelial-mesenchymal transition (EMT) program. DEAR1 is a tumor suppressor gene which is mutated, undergoes loss of heterozygosity in breast cancer, and is downregulated in DCIS …

Involvement Of Gdap1 In The Regulation Of Mtorc1 Activity In A Drosophila Mliv Model, Kristen Clemons

Dissertations and Theses (Open Access)

The master regulator of metabolism and growth, mechanistic target of rapamycin complex 1 (mTORC1), is responsible for maintaining metabolic homeostasis by sensing nutrient and energy levels within the cell to promote or inhibit translation and autophagy accordingly. In the childhood neurodevelopmental disorder Mucolipidosis type IV (MLIV), mTORC1 activity is decreased. The underlying mechanism for reduced mTORC1 signaling in MLIV is poorly understood. The gene encoding ganglioside-induced differentiation associated protein 1 (GDAP1) is transcriptionally upregulated in MLIV. This project investigated the involvement of GDAP1 in MLIV disease pathology. Using the UAS/GAL4 system in an established MLIV Drosophila model, we …

A Compressed Sensing Approach To Detect Immobilized Nanoparticles Using Superparamagnetic Relaxometry, Sara Thrower

Dissertations and Theses (Open Access)

Superparamagnetic relaxometry (SPMR) is an emerging technology that leverages the unique properties of biologically targeted superparamagnetic iron oxide nanoparticles to detect cancer. The use of ultra-sensitive sensors enables SPMR to detect tumors ten times smaller than current imaging methods. Reconstructing the distribution of cancer-bound nanoparticles from SPMR measurements is challenging because the inverse problem is ill posed. Current methods of source reconstruction rely on prior knowledge of the number of clusters of bound nanoparticles and their approximate locations, which is not known in clinical applications. In this work, we present a novel reconstruction algorithm based on compressed sensing methods that …

Role Of P300 Zz Domain In Chromatin Association And Histone Acetylation, Yongming Xue

Dissertations and Theses (Open Access)

Transcription is strictly regulated by numerous factors including transcription coactivators. The p300 protein and its close paralogue CREB-binding protein (CREBBP, aka CBP) are well-known transcriptional coactivators that have intrinsic lysine acetyltransferase activity. The functions of p300/CBP largely rely on their capabilities to bind to chromatin and to acetylate the histone substrates. However, the molecular mechanisms underlying the regulation of these processes are not fully understood.

Through combination of various biochemical, biophysical and molecular approaches, we show that the ZZ-type zinc finger (ZZ) domain of p300 functions as a histone reader that specifically binds the N-terminal tail of histone H3. Crystal …

Behavioral Insights Into Nociceptor Function: A Systematic Approach To Understanding Postsurgical And Neuropathic Pain Mechanisms In Rats, Max Odem

Dissertations and Theses (Open Access)

Postsurgical and neuropathic pain are each clinically common, and often associated with ongoing pain. Ongoing pain has been linked to ongoing activity (OA) in human C-fiber nociceptors. Preclinical studies using rodent neuropathic models have concentrated on allodynia driven by OA generated in non-nociceptive Aβ fibers, but little attention has been paid to postsurgical pain in sham controls or to C-fiber nociceptor OA promoting ongoing pain.

Operant assays that reveal negative motivational and cognitive aspects of voluntary pain-related behavior may be particularly sensitive to pain-related alterations. In the mechanical conflict (MC) test, rodents can freely choose to escape from a brightly …

A Role For Epac1 And Epac2 In Nociceptor Hyperexcitability And Chronic Pain After Spinal Cord Injury, Samantha Berkey

Dissertations and Theses (Open Access)

Chronic pain is a major complaint of those living with spinal cord injury (SCI), affecting 65-80% of the SCI population, but the treatment options remain limited or non-existent. The cAMP sensor EPAC has previously been shown to play a key role in chronic inflammatory and neuropathic pain, though the contribution from each of its two main isoforms, EPAC1 and EPAC2, is unclear. Here I test the hypothesis that both EPAC1 and EPAC2 play a key role in the maintenance of persistent nociceptor hyperexcitability and chronic pain after SCI.

Using both a T9 SCI mouse model and a T10 SCI rat …

Adenylyl Cyclase Type 9: Regulation And Cardiac Function, Tanya A. Baldwin, Tanya A. Baldwin

Dissertations and Theses (Open Access)

Abnormalities in cardiac stress signaling underlie a number of cardiovascular diseases (e.g. arrhythmias and heart failure). Cardiac stress signaling pathways normally integrate signals from the sympathetic nervous system to promote efficient contraction and relaxation under stress. Sympathetic control through β-adrenergic stimulation is propagated by adenylyl cyclase (AC). AC synthesizes cyclic AMP (cAMP), an important second messenger that initiates signaling pathways to modulate physiological and pathophysiological functions of the heart, including the activation of PKA and subsequent phosphorylation of ion channels, contractile machinery, and stress response proteins that enhance cardiac function. Alterations of cAMP signaling occur in the failing heart and …

3d Scintillator Detector Quenching Characterization For Scanning Proton Beams, Fahed Alsanea

Dissertations and Theses (Open Access)

Proton pencil beam scanning is becoming the standard treatment delivery technique for proton therapy centers. Scanned proton pencil beams provide a highly conformal dose distribution. The complex dose distribution poses challenges for quality assurance measurements leading to sophisticated detector setups and time consuming measurements. Fast 3D measurements are therefore desirable to verify the complex dose distribution and to enable the utilization of the full potential of proton therapy. The overall objective of this project is to improve volumetric scintillators detectors to provide 3D measurements for applications for beam commissioning, quality assurance program, and patient-specific treatment delivery verification.

Detectors based on …

Integrative Bioinformatics Approaches To Elucidating Prostate Cancer Cell Heterogeneity, Plasticity, And Treatment Response, Hsueh-Ping Chao

Dissertations and Theses (Open Access)

Prostate cancer (PCa) is the most common non-cutaneous tumor in American men, and the second leading cause of cancer-related deaths. PCa-related deaths can be attributed to heterogeneous tumors containing metastatic and therapy-resistant cancer cells. Cancer stem cells (CSC) are an important contributor to this tumor heterogeneity, which are present in primary tumors and become enriched in castration resistant PCa (CRPC). Our lab has demonstrated that the prostate cancer stem cells (PCSCs) are enriched in the phenotypically undifferentiated PCa cell population that lacks the expression of differentiation marker prostate-specific antigen (PSA). Our work has also demonstrated that PCa cells manifest significant …

Investigation Of Novel Virulence Mechanisms In Candida Albicans, Elisa Marie Tafoya Vesely

Dissertations and Theses (Open Access)

Candida albicans is the most important fungal species associated with humans. Normally present in the human microbiome as a commensal colonizer, C. albicans is also the fourth most prevalent organism isolated from bloodstream infections in hospitals. Disseminated infections have an associated mortality rate of around 40%. The results of the work described in this dissertation cover the broad subject of C. albicans adaptation to the host and the mechanisms by which this organism is able to survive and cause infection through a variety of means. A key determinant in disease progression is interaction with innate immune cells, specifically macrophages and …

Improving Dbnsfp, Mingyao Lu

Dissertations and Theses (Open Access)

IMPROVING dbNSFP

Mingyao Lu, B.S.

Advisory Professor: Xiaoming Liu, Ph.D.

The analysis and interpretation of DNA variation are very important for the Whole Exome studies (WES). Genome research has focused on single nucleotide variants (SNVs). Since indels are as important as SNVs, especially indels in coding regions are often candidates of disease-causing variants, thus, it is necessary to expand the focus to include indel mutations.

The goal of my project is to provide an automatic annotation pipeline to the WES based disease studies project by extending the dbNSFP with a tool for automated indel annotation and deleteriousness prediction. The current …

Il-1Α Blockade Reduces Immune Suppression In The Early Tumor Micro-Environment, Brenda Melendez

Dissertations and Theses (Open Access)

IL-1α Blockade Reduces Immune Suppression in the Early Tumor Micro-Environment

Brenda Melendez, B.S.

Advisory Professor: Gregory Lizee, PhD.

Immunotherapy against melanoma has shown great promise in the clinic for treating advanced-stage patients. However, a major barrier against effective T cell mediated cytotoxicity is immunosuppression in the tumor micro-environment. It has been described that tumors secrete pro-inflammatory cytokines capable of modulating immune responses that favors the growth of tumor cells. Specifically, IL-1 plays a critical role in myeloid cell recruitment and activation, which can in turn inhibit T cell activity in vivo. Moreover, IL-1 is also known to up-regulate immune inhibitory …

Heme Peroxidase Hpx-2 Protects Caenorhabditis Elegans From Pathogens, Yi Liu

Dissertations and Theses (Open Access)

Heme-containing peroxidases are important components of innate immunity. Many of them functionally associate with NADPH oxidase (NOX)/dual oxidase (DUOX) enzymes by using the hydrogen peroxide they generate in downstream reactions. In Caenorhabditis elegans, a ShkT-containing peroxidase, SKPO-1, and the dual oxidase, BLI-3, contribute to resistance against the human pathogen Enterococcus faecalis, likely functioning in conjunction with each other. However, the exact mechanism(s) by which peroxidases and NOX/DUOXs contribute to pathogen resistance remain unclear. Here, we demonstrated that another peroxidase, HPX-2 (Heme-PeroXidase 2), is required for resistance against some, but not all pathogens. Tissue specific …