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The Impact Of The Deepwater Horizon Blowout On Historic Shipwreck-Associated Sediment Microbiomes In The Northern Gulf Of Mexico, Leila J. Hamdan, Jennifer Salerno, Allen H. Reed, Samantha B. Joye, Melanie Damour

Faculty Publications

More than 2,000 historic shipwrecks spanning 500 years of history, rest on the Gulf of Mexico seafloor. Shipwrecks serve as artificial reefs and hotspots of biodiversity by providing hard substrate, something rare in deep ocean regions. The Deepwater Horizon (DWH) spill discharged crude oil into the deep Gulf. Because of physical, biological, and chemical interactions, DWH oil was deposited on the seafloor, where historic shipwrecks are present. This study examined sediment microbiomes at seven historic shipwrecks. Steel-hulled, World War II-era shipwrecks and wooden-hulled, 19th century shipwrecks within and outside of the surface oiled area and subsurface plume were examined. Analysis …

Quality Changes Of Salmon Fillet With Different Packagings During The Storage, Shen Qiuxia, Li Mingyuan, Hu Yongzheng, Guo Jichuan, Wang Xiaojun, Lu Chaoting, Ji Li, Wen Xiaohui, Ding Wenwu

Food and Machinery

In order to explore the physico-chemical quality changes of vacuum packaging and nitrogen-filled packaging salmon fillet stored at 4 ℃ and 10 ℃, and some indicators were analyzed periodically, including the sensory evaluation, texture, pH value, and the values of TVB-N and TBA. Volatile components of salmon fillet with different treatments, detected by an electronic nose, were analyzed by loading analysis (LA) and principal component analysis (PCA). The results showed that the sensory scores, elasticity, resilience and chewiness values decreased gradually with the extension of storage time; TVB-N values and TBA values decreased gradually, while hardness values of fillet increased …

Optimization Of Steam Explosion Pretreatment For Polysaccharides Extraction From Achyranthes Bidentata Radix And Of Its Antioxidant Activity Evaluation, Yi Junpeng, Wang Sai, Li Xin, Yang Yahuang, Zhao Pengcheng, Zhu Wenxue

Food and Machinery

Extraction yield of polysaccharides from Achyranthes bidentata Radix (AbR) was increased by steam explosion pretreatment. Based on the results of single-factor experiments, the response surface methodology was employed to further optimize the steam explosion pretreatment conditions. The effect of steam explosion pretreatment on the DPPH radical scavenging activity and O^2- radical scavenging activity of Achyranthes bidentata Polysaccharides (AbPS) was evaluated. The optimum conditions was determined as follows: the water/AbR ratio 11%, steam explosion pressure, 1.6 MPa; residence time 63 s. After pretreatment under the optimum conditions, the extraction yield of polysaccharides from AbR was found to be 11.88%, which …

Study On The Relationship Between The Composition Of Tea Extracts And Its In Vitro Antioxident Activity, Li Dan, Cao Yong, Wang Hua, Feng Yunzi, Wu Jinming, Zhao Mouming

Food and Machinery

In this study, Maojian, crushed black tea and zymic Pu'er tea were chosen as representative of non-fermented, fully-fermented and post-fermented tea, respectively. The composition profiles of ethanol/water extracts were compared, and the antioxidant activities in vitro were detected by oxygen radical absorbance capacity (ORAC) and DPPH assay. In order to confirm the contribution of each key functional contributors, recombination model of antioxidant compounds in tea have been built and studied. The results showed that ethanol extracts contained higher contents of tea polyphenol, caffeine and prolamin, and higher antioxidant activities detected by ORAC and DPPH assay, while higher amounts of tea …

Research Progress Of Extraction, Chemical Composition And Function Of Hemp Seed Oil, Chen Zehua, Chen Tong, Li Wei, Liu Fei, Du Jie, Feng Konglong, Cao Yong

Food and Machinery

Hemp seed oil has a good application prospects in function of hypolipidemic, anti-oxidation and anti-aging which can meet the needs of people's diet health. This review summarized different extraction methods and chemical components of Hemp oil, and the research on biological activity, looking forward to the development prospects of Hemp oil and providing the basis for its development and utilization.

Optimization Of The Preparation Process Of Hydroxyl Radicals Based On Impinging Stream-Jet Cavitation Effect, Ren Xiaomin, Huang Yongchun, Yang Feng, Huang Chengdu

Food and Machinery

In order to study the free radical yield variation law of impinging stream-jet cavitation effects and its influence factors, and to better apply in the field of food processing, the impinging stream-jet cavitation was used to be compared with single impinging stream and single jet cavitation respctively. The effects of upstream pressure, solution temperature, cavitation time and other factors on the yield of hydroxyl radicals (·OH) were investigated. The interaction between factors and their optimum conditions were analyzed by response surface analysis. The experimental results showed that ·OH yield increased first and then decreased with the increase of the pressure …

Screening And Identification Of Special Yeast For Kiwi Wine, Jiang Cheng, Wei Miaohong, Liu Lu, Zhao Yaqin, Zhao Jianglin, Hou Xiaoyan, Chen Anjun

Food and Machinery

In order to screen raw and low-yield methanol kiwifruit yeast and provide special strains resources to the kiwifruit wine production. Strains were identified by colony morphology and 18S rDNA sequencing to determine the species relationship of the three strains of yeast. The fermentation capacity, alcohol and sulfur dioxide tolerance were evaluated. Additionally, the three strains were used for kiwifruit wine fermentation, and the physicochemical properties, methanol content and volatile flavor compounds of the product were determined. The three strains were identified as Pichia guilliermondii by molecular biology. Among them, Q3 had the strongest fermentation capacity, it could tolerate 16% of …

Pro-Inflammatory Cytokines Attenuate Glucose-Stimulated Insulin Secretion From Ins-1e Insulinoma Cells By Restricting Mitochondrial Pyruvate Oxidation Capacity - Novel Mechanistic Insight From Real-Time Analysis Of Oxidative Phosphorylation., Jonathan Barlow, Thomas P.J. Solomon, Charles Affourtit

School of Biomedical Sciences

Pro-inflammatory cytokines cause pancreatic beta cell failure during the development of type 2 diabetes. This beta cell failure associates with mitochondrial dysfunction, but the precise effects of cytokines on mitochondrial respiration remain unclear. To test the hypothesis that pro-inflammatory cytokines impair glucose-stimulated insulin secretion (GSIS) by inhibiting oxidative ATP synthesis, we probed insulin release and real-time mitochondrial respiration in rat INS-1E insulinoma cells that were exposed to a combination of 2 ng/mL interleukin-1-beta and 50 ng/mL interferon-gamma. We show that 24-h exposure to these cytokines dampens both glucose- and pyruvate-stimulated insulin secretion (P < 0.0001 and P < 0.05, respectively), but does not affect KCl-induced insulin release. Mirroring secretory defects, glucose- and pyruvate-stimulated mitochondrial respiration are lowered after cytokine exposure (P < 0.01). Further analysis confirms that cytokine-induced mitochondrial respiratory defects occur irrespective of whether fuel oxidation is coupled to, or uncoupled from, ATP synthesis. These observations demonstrate that pro-inflammatory cytokines attenuate GSIS by restricting mitochondrial pyruvate oxidation capacity. Interleukin-1-beta and interferon-gamma also increase mitochondrial superoxide levels (P < 0.05), which may reinforce the inhibition of pyruvate oxidation, and cause a modest (20%) but significant (P < 0.01) loss of INS-1E cells. Cytokine-induced INS-1E cell failure is insensitive to palmitoleate and linoleate, which is at odds with the cytoprotection offered by unsaturated fatty acids against harm caused by nutrient excess. Our data disclose a mitochondrial mechanism for cytokine-impaired GSIS in INS-1E cells, and suggest that inflammatory and nutrient-related beta cell failure emerge, at least partly, through distinct paths.

Two Critical Positions In Zinc Finger Domains Are Heavily Mutated In Three Human Cancer Types, Daniel Munro, Dario Ghersi, Mona Singh

Interdisciplinary Informatics Faculty Publications

A major goal of cancer genomics is to identify somatic mutations that play a role in tumor initiation or progression. Somatic mutations within transcription factors are of particular interest, as gene expression dysregulation is widespread in cancers. The substantial gene expression variation evident across tumors suggests that numerous regulatory factors are likely to be involved and that somatic mutations within them may not occur at high frequencies across patient cohorts, thereby complicating efforts to uncover which ones are cancerrelevant. Here we analyze somatic mutations within the largest family of human transcription factors, namely those that bind DNA via Cys2His2 zinc …

Sexual Expression And Social Connectedness Among Community-Dwelling Older Adults, In The United States, Neal J. Blangiardo

Dissertations and Theses

Sexuality is a central aspect of human identity for all people, including older persons. Sexual expression continues across the lifespan, despite the cultural fallacy of the sexlessness of older adults. Older adults live active sexual lives which involve relationships, social roles, and an array of sexuality-related needs.

This dissertation examines the relationship between sexual expression and social connectedness, in older community-dwelling adults. Research on sexuality in this community predominately focuses on genital sexual expression, has not explored the non-genital dimension of sexual expression, and little is known about the role of social relationships that can play in supporting sexual expression. …

Direct Cell-To-Cell Transmission Of Respiratory Viruses: The Fast Lanes, Nicolás P. Cifuentes-Muñoz, Rebecca Ellis Dutch, Roberto Cattaneo

Molecular and Cellular Biochemistry Faculty Publications

Virus particles protect genomes from hostile environments within and outside the host, eventually delivering these genomes to target tissues to initiate infection. Complex processes requiring significant energy and time are necessary to assemble these virus particles, but only a small portion of released virus will successfully infect new target cells (Fig 1A). While the science of virology has developed based on the isolation and purification of viral particles, it is becoming increasingly clear that direct cell-to-cell transmission of viruses and/or viral components is also highly relevant [1,2].

Direct cell-to-cell spread of infections has several advantages. The first is efficiency: genomic …

Novel Role Of Prostate Apoptosis Response-4 Tumor Suppressor In B-Cell Chronic Lymphocytic Leukemia, Mary Kathryn Mckenna, Sunil K. Noothi, Sara S. Alhakeem, Karine Z. Oben, Joseph T. Greene, Rajeswaran Mani, Kathryn L. Perry, James P. Collard, Jacqueline R. Rivas, Gerhard C. Hildebrandt, Roger A. Fleischman, Eric B. Durbin, John C. Byrd, Chi Wang, Natarajan Muthusamy, Vivek M. Rangnekar, Subbarao Bondada

Microbiology, Immunology, and Molecular Genetics Faculty Publications

Prostate apoptosis response-4 (Par-4), a proapoptotic tumor suppressor protein, is downregulated in many cancers including renal cell carcinoma, glioblastoma, endometrial, and breast cancer. Par-4 induces apoptosis selectively in various types of cancer cells but not normal cells. We found that chronic lymphocytic leukemia (CLL) cells from human patients and from Eµ-Tcl1 mice constitutively express Par-4 in greater amounts than normal B-1 or B-2 cells. Interestingly, knockdown of Par-4 in human CLL-derived Mec-1 cells results in a robust increase in p21/WAF1 expression and decreased growth due to delayed G1-to-S cell-cycle transition. Lack of Par-4 also increased the expression of p21 and …

Tanapox: A Potential Oncolytic Virus For The Treatment Of Cancer, Joel Marty

Honors Theses

The National Cancer Institute defines cancer as a group of related diseases characterized by unimpeded cell division with metastatic abilities. Cells frequently mutate and potentially become cancerous, but generally these cells are cleared by the immune system. The disease state of cancer arises when the mutated cells evade the immune clearance, which indicates medical intervention is required. Current therapies most commonly utilize chemotherapy, radiation, or a combination of the two, however, alternative therapies are being developed such as immunotherapies and oncolytic viruses (OVs). OVs cause a viral infection, clearing cancerous cells through viral replication mechanisms, such as viral cytolysis, while …

Comparison Of The Content Of Main Active Components In Sweet Potato Leaves, Purple Potato Tuber And Purple Potato Leaves Of Different Development Stages, Xu Ke, Zeng Fankun, Yuan Mei, Luo Jingwen

Food and Machinery

The dynamic changes of the main active components in purple potato leaves were studied. The optimal sampling period was determined, and the content of main active components in purple potato leaves, sweet potato leaves and purple potato tuber were compared. The content of total polyphenols, flavonoids and anthocyanins were determined by ultraviolet spectrophotometer, and chlorogenic acid and rutin were determined by HPLC. The results showed that the dynamic characteristics of active components in different varieties of purple sweet potato leaves showed a similar trend, but the content were not exactly consistent in different sampling time, and the content of active …

Effect Of Heat Transfer Characteristics Of Cookware On The Control Of Chinese Cooking Operation, Peng Jing, Deng Li, Wang Lei, He Congying, Yu Bingyan, Lin Jin, Cui Jie

Food and Machinery

To obtain the regularity between the heat transfer characteristics of wok and the control of Chinese cooking operation and establish a Chinese wok performance evaluation method, three kinds of Chinese woks were selected to study the influence of the thermal resistance coefficient on the maturation time and the initial scorching time of pork tenderloin at different preheating temperature and mixing frequency. The results showed that the quality characteristics of pork tenderloin, such as color, moisture content and shear force, were obviously changed when it was fry to matured and scorched, and the color could be used as an intuitive judgment …

Study On The Extraction Technology, Physicochemical Properties And Bioactivity Of The Polysaccharide Form Radix Glehniae, Jing Yongshuai, Su Lei, Han Yu, Zhang Danshen, Zhang Ruijuan, Wu Lanfang, Zheng Yuguang, Qin Xuan

Food and Machinery

Response surface methodology based on single-factor experiment was applied to optimize the extraction conditions of polysaccharide. As a result, the optimum extraction conditions were extraction temperature 65 ℃, ultrasonic time 22 min, liquid to material ratio of 221 （mL/g）. Under the control of such conditions, the extraction rate of polysaccharide from Radix Glehniae was 12.13%. The polysaccharide from Radix Glehniae had typical characteristics of polysaccharides, containing 90.4% of the total sugar and 1.22% protein, and the thermal degradation temperature was 300.32 ℃. The scavenging abilities of DPPH free radicals (IC₅₀=1.99 mg/mL), OH free radicals (IC₅₀=1.71 mg/mL) …

Biosorption Of Cobalt By Using Pseudomonas Aerguinosa Bacterial Strain, Abhaysinh Arvind Dharanguttikar

USF Tampa Graduate Theses and Dissertations

A study of biosorption of cobalt metal by Pseudomonas Aerguinosa gram-negative bacterial strain is presented. The present study is carried out to determine the optimum conditions of cobalt biosorption at ultra-low concentration (ppb range) in aqueous solutions. The receptiveness of cobalt metal on the extracellular surface of bacterial strain was examined by varying the pH, Initial concentration of metal and treatment time. Experimental data showed that effect of pH and treatment time is prevalent in biosorption of cobalt and by increasing both these parameters resulted in the efficient sorption of cobalt on the extracellular surface of Pseudomonas Aerguinosa. In some …

Chemical Investigation Of Bioactive Marine Extracts, Selam Hagos

USF Tampa Graduate Theses and Dissertations

Natural products have been a fundamental source of medicinal scaffolds for decades; with sixty percent of marketed drugs. Many synthetic chemists are focused on synthesizing potent and nontoxic compounds for pharmaceutical targets, however, nature is still proving to be a source of new bioactive compounds. Produced by the host organism for defense, reproduction and communication, secondary metabolites also demonstrate promising bioactivity against human pathogens. Hence, natural product chemists continue their quest for new leads.

As a continuation of these efforts, this thesis attempts to explore fungi and sponges for new chemistry, and ultimately, new drug candidates. Antarctica is largely untapped; …

Development Of The Cybersecurity Attitudes Scale And Modeling Cybersecurity Behavior And Its Antecedents, David J. Howard

USF Tampa Graduate Theses and Dissertations

As organizations have become more reliant on computers and technology to operate in a globalized world, they have also become more vulnerable to cyberattacks on their networks. The expense to organizations from cyberattacks now exceeds $400 billion USD annually. These costs highlight the need for behavioral research in the cyber domain. The first phase of this research developed an instrument to measure workers’ cybersecurity attitudes. An iterative process resulted in a scale with good psychometric properties - The Cybersecurity Attitudes Scale. The scale measures two factors: cyber policy adherence attitudes and perceived vulnerability to a cyberattack. The second phase of …

An Evaluation Of Marsh Shoreline Erosion And Sediment Deposition In The Grand Bay National Estuarine Research Reserve, Mississippi, Usa, Joseph Terrano

USF Tampa Graduate Theses and Dissertations

Coastal marshes serve important ecologic and economic functions, such as providing habitat, absorbing floodwaters and storm surges, and sequestering carbon. Throughout the northern Gulf of Mexico, coastal marshes are disappearing due to wave attack, sea-level rise, sediment export, and subsidence. Marsh area increases when sediments accumulate at the marsh shoreline, accrete vertically, and when non-marsh areas are colonized by marsh vegetation. Marsh shoreline erosion results in net marsh loss when transgression rates at the marsh-water edge exceed upland-marsh migration. The balance between marsh destroying and marsh creating processes determines the long-term survivability of a marsh system. Thus, processes of shoreline …

Diversity Of Ssdna Phages Related To The Family Microviridae Within The Ciona Robusta Gut, Alexandria Creasy

USF Tampa Graduate Theses and Dissertations

The gut microbiome is a complex ecosystem of bacteria, viruses, and fungi that strongly influences animal health. The bacterial component, for example, contributes orders of magnitude more gene products to host physiology than the host genome; thus, changes to the composition of these bacterial communities can have profound influences on the health of the animal. By infecting and lysing their hosts, viruses (particularly viruses infecting bacteria or phages) can affect critical functions in these environments, yet the consequences of these infections remain to be fully described. Most studies investigating gut viromes to date have focused on double-stranded DNA (dsDNA) phages …

Therapeutic Modulation Of Alzheimer’S Disease With Biological (Hucbs) And Pharmacological (Lispro) Approaches, Md Ahsan Habib

USF Tampa Graduate Theses and Dissertations

Dementia is the top global public health threat of the twenty first century. Within the dementia spectrum, Alzheimer’s disease (AD) is the most common type of dementia that occurs with aging and accounts for about 60% - 80% of diagnosed cases. But currently available discoveries failed to develop disease-modifying therapies for all patients living with AD. Recent discoveries can only partially slow down cognitive decline in a small subset of patients with limited effectiveness. The heterogeneity and complexity of the pathophysiology of AD indicate that a single drug approach may not be sufficient to prevent disease onset and progression. Human …

Contact Prediction Is Hardest For The Most Informative Contacts, But Improves With The Incorporation Of Contact Potentials, Jack Holland, Qinxin Pan, Gevorg Grigoryan

Dartmouth Scholarship

Co-evolution between pairs of residues in a multiple sequence alignment (MSA) of homologous proteins has long been proposed as an indicator of structural contacts. Recently, several methods, such as direct-coupling analysis (DCA) and MetaPSICOV, have been shown to achieve impressive rates of contact prediction by taking advantage of considerable sequence data. In this paper, we show that prediction success rates are highly sensitive to the structural definition of a contact, with more permissive definitions (i.e., those classifying more pairs as true contacts) naturally leading to higher positive predictive rates, but at the expense of the amount of structural information contributed …

Super‐Resolution Imaging Of Amyloid Structures Over Extended Times By Using Transient Binding Of Single Thioflavin T Molecules, Kevin Spehar, Tianben Ding, Yuanzi Sun, Niraja Kedia, Jin Lu, George R. Nahass, Matthew D. Lew, Jan Bieschke

Electrical & Systems Engineering Publications and Presentations

Oligomeric amyloid structures are crucial therapeutic targets in Alzheimer's and other amyloid diseases. However, these oligomers are too small to be resolved by standard light microscopy. We have developed a simple and versatile tool to image amyloid structures by using thioflavin T without the need for covalent labeling or immunostaining. The dynamic binding of single dye molecules generates photon bursts that are used for fluorophore localization on a nanometer scale. Thus, photobleaching cannot degrade image quality, allowing for extended observation times. Super‐resolution transient amyloid binding microscopy promises to directly image native amyloid by using standard probes and record amyloid dynamics …

Isolation And Identification Of Antagonistic Bacteria Aganist Vibrio Parahaemolyticus And Its Characteristics Of Antibacterial Substances, Hao Yanli, Wang Liping, Huang Yuliang, Xue Meicui, Cui Yunyun, Zhao Yong, Li Li, Wang Zhengquan

Food and Machinery

In order to screen a probiotic strain against foodborne pathogens and aquatic pathogens, the main zoonotic pathogenic bacteria of mariculture Vibrio parahaemolyticus ATCC17802 was used as indicator bacterium. The target probiotics was screened using the spot-on-lawn method and rescreened by the agar diffusion method, and the diameter of inhibition zone of antibacterial substances reached (17.16±0.28) mm. Through the combination of morphology and 16S rDNA sequence analysis, strain H19 was identified as Bacillus subtilis (GenBank accession number: MG383451). Antimicrobial spectrum of B. subtilis H19 was tested, meanwhile the physical and chemical properties of antimicrobial substances produced by H19 was studied. The …

Roles Of Plods In Collagen Synthesis And Cancer Progression, Yifei Qi, Ren Xu

Markey Cancer Center Faculty Publications

Collagen is the major component of extracellular matrix. Collagen cross-link and deposition depend on lysyl hydroxylation, which is catalyzed by procollagen-lysine, 2-oxoglutarate 5-dioxygenase (PLOD). Aberrant lysyl hydroxylation and collagen cross-link contributes to the progression of many collagen-related diseases, such as fibrosis and cancer. Three lysyl hydroxylases (LH1, LH2, and LH3) are identified, encoded by PLOD1, PLOD2, and PLOD3 genes. Expression of PLODs is regulated by multiple cytokines, transcription factors and microRNAs. Dysregulation of PLODs promotes cancer progression and metastasis, suggesting that targeting PLODs is potential strategy for cancer treatment. Here, we summarize the recent progress in the investigation of …

Climate And Plant Controls On Soil Organic Matter In Coastal Wetlands, Michael J. Osland, Christopher A. Gabler, James B. Grace, Richard H. Day, Meagan L. Mccoy, Jennie L. Mcleod, Andrew S. From, Nicholas M. Enwright, Laura C. Feher, Camille L. Stagg

School of Earth, Environmental, & Marine Sciences Faculty Publications

Coastal wetlands are among the most productive and carbon‐rich ecosystems on Earth. Long‐term carbon storage in coastal wetlands occurs primarily belowground as soil organic matter (SOM). In addition to serving as a carbon sink, SOM influences wetland ecosystem structure, function, and stability. To anticipate and mitigate the effects of climate change, there is a need to advance understanding of environmental controls on wetland SOM. Here, we investigated the influence of four soil formation factors: climate, biota, parent materials, and topography. Along the northern Gulf of Mexico, we collected wetland plant and soil data across elevation and zonation gradients within 10 …

Harvesting Invasive Plants To Reduce Nutrient Loads And Produce Bioenergy: An Assessment Of Great Lakes Coastal Wetlands, Brendan D. Carson, Shane C. Lishawa, Nancy C. Tuchman, Andrew M. Monks, Beth A. Lawrence, Dennis A. Albert

School of Environmental Sustainability: Faculty Publications and Other Works

In Laurentian Great Lakes coastal wetlands (GLCWs), dominant emergent invasive plants are expanding their ranges and compromising the unique habitat and ecosystem service values that these ecosystems provide. Herbiciding and burning to control invasive plants have not been effective in part because neither strategy addresses the most common root cause of invasion, nutrient enrichment. Mechanical harvesting is an alternative approach that removes tissue‐bound phosphorus and nitrogen and can increase wetland plant diversity and aquatic connectivity between wetland and lacustrine systems. In this study, we used data from three years of Great Lakes‐wide wetland plant surveys, published literature, and bioenergy analyses …

Computational Analysis Of Papionini Evolution Using Alu Insertions, Vallmer Edward Jordan Ii

LSU Doctoral Dissertations

Alu elements are primate specific retrotransposons that have remained active throughout the course of primate evolution. As a result of this sustained mobilization. Alu elements are present in greater copy number in primate genomes than any other transposable element. An average of over one million Alu elements has been identified in every sequenced haplorrhine genome to date. These characteristics qualify Alu elements as ideal characters for studying evolutionary relationship among primates.

The increasing availability of whole genome sequencing data presents novel challenges and opportunities for comparative genomic analyses. Genomic data is now publicly available for most primate species. Such an …