Digital Commons Network^™

Identification And Engineering Of Nonribosomal Peptide Biosynthetic Systems, Fuchao Xu

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

This research focuses on the understanding and engineering of nonribosomal peptide biosynthetic pathways in Streptomyces coelicolor CH999, Escherichia coli BAP1 and Saccharomyces cerevisiae BJ5464-NpgA. The biosynthetic systems of indigoidine from bacteria and beauvericin/bassianolide from fungi were studied in this research. The production of these valuble products was significantly increased by enhancing their synthetic pathway with metabolic engineering approaches.

Indigoidine is a bacterial natural product with antioxidant and antimicrobial activities. Its bright blue color resembles the industrial dye indigo, thus representing a new natural blue dye that may find uses in industry. Indigo is a dark blue crystalline powder and has …

Investigating Rapid Concrete Repair Materials And Admixtures, Ivan Quezada

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

This dissertation presents a literature review of the state-of-practice for the use of IC in concrete mixtures and how structural engineers and construction engineers can adapt IC to their present and future work. Current high early strength concrete mixtures have natural cracking and shrinkage problems due to the high content of cementitious material or their chemical components. Using IC allows for early strength, enhanced durability, reduced shrinkage and a better curing by providing water that can be absorbed by the cement past after the final set. Rapid hydration and high early strength Portland cement and calcium sulfoaluminate (CSA) concretes are …

Production And Purification Of Synthetic Minor Ampullate Silk Proteins, Danielle A. Gaztambide

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Spider silks are incredible natural materials that have a wide variety of properties that can rival or outperform even common synthetic materials like Nylon and Kevlar. As nature’s architects, orb-weaving spiders spin seven different silks that are used for very specific roles throughout the spider’s lifecycle. These silks are comprised of proteins called spidroins. Each of these spidroins has evolved to have properties such as strength and/or stretch that make these silks successful and highly adapted in their designated roles in web construction, prey capture and reproduction.

This study involves the production of minor ampullate silk by genetically modifying the …

A Needs Assessment Of Arizona Agricultural Education Equine Science Curriculum, Emily Tobler

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Like any other agricultural industry, many different career paths in the equine field are becoming more technologically advanced. To adapt to technological progress, current standards need to be integrated into school-based agricultural education courses to properly train and teach students about current industry standards. In addition to equine science curriculum development, emphasis must also be focused on providing assistance to secondary-school-based agriculture teachers so they can effectively teach industry-based equine science objectives in their programs.

The equine curriculum objectives were measured in terms of perceived importance by equine professionals and post-secondary equine educators as well as Arizona school-based agriculture teachers. …

Additively-Manufactured Hybrid Rocket Consumable Structure For Cubesat Propulsion, Britany L. Chamberlain

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Three-dimensional, additive printing has emerged as an exciting new technology for the design and manufacture of small spacecraft systems. Using 3-D printed thermoplastic materials, hybrid rocket fuel grains can be printed with nearly any cross-sectional shape, and embedded cavities are easily achieved. Applying this technology to print fuel materials directly into a CubeSat frame results in an efficient, cost-effective alternative to existing CubeSat propulsion systems. Different 3-D printed materials and geometries were evaluated for their performance as propellants and as structural elements. Prototype "thrust columns" with embedded fuel ports were printed from a combination of acrylonitrile utadiene styrene (ABS) and …

An Examination Of What Motivates Utah Residents To Adopt The Practice Of Rainwater Harvesting, D. Wayne Honaker

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Although most of the earth is covered in water, a very limited amount of that water is fresh water, which is essential to our survival. Therefore, it is imperative that we do all that is possible to conserve and protect our extremely limited water resources, especially in arid regions such as the American West. While there are many ways and means to protecting and preserving our water resources, this thesis focuses on the strategy of rainwater harvesting (RWH) as it is done throughout the state of Utah. RWH is defined as taking the precipitation that falls on our built structures …

The Effects Of Didactic Training & Behavioral Skills Training On Staff Implementation Of A Stimulus Preference Assessment With Adults With Disabilities, Sandra G. Smith

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

This study assessed the effects of BST and DT on the treatment integrity scores of four direct care staff (DCS) conducting a multiple stimulus without replacement assessment with confederates. A concurrent multiple baseline design showed that BST was effective in achieving mastery level treatment integrity scores for all participants and that these scores generalized to adults with disabilities and remained at mastery levels after a 2-week generalization phase.

Interactions Between Fire Severity And Forest Biota In The Central Sierra Nevada: Formation And Impact Of Small-Scale Fire Refugia And The Effect Of Fire On Forest Structure Predictive Of Fisher (Pekania Pennanti) Den Habitat, Erika M. Blomdahl

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Fire is a natural and essential component of forests in western North America. Fire maintains biodiversity through the creation of different habitat types, and regular fire rotations reduce the accumulation of woody fuels and thick understory plant densities that give rise to catastrophic fire. The practice of fire exclusion has altered western forests and increased the risk of widespread change under rising temperatures projected for the 21^st century. To manage for the reintroduction of fire it is critical that we understand the interactions between fire and forest biota in recently fire-suppressed forests.

In Chapter 2, I studied the formation …

Quantum Mechanical Studies Of N-H···N Hydrogen Bonding In Acetamide Derivatives And Amino Acids, Sandra J. Lundell

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Proteins are made of vast chains of amino acids that twist and fold into intricate designs. These structures are held in place by networks of noncovalent interactions. One of these, the hydrogen bond, forms bridges between adjacent pieces of the protein chain and is one of the most important contributors to the shape and stability of proteins. Hydrogen bonds come in all shapes and sizes and a full understanding of these not only aids in our understanding of proteins in general but can bridge the gap to finding cures to many protein-related diseases, such as sickle-cell anemia. The primary aim …

To Be, Or Not To Be (Like My Sibling), That Is The Question: Examining Modeling And Differentiation Behaviors Among Siblings In Organized Youth Sport, Keith Vakafutu Osai

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Sibling relationships are typically the most enduring relationship in the family unit. A large body of research documents how sibling relationships occur in the context of the immediate family, how they impact behaviors such as risk-taking, how different cultures view siblings, and how similarities and differences among siblings can be attributed to genetics as well as shared and non-shared experiences. However, one relatively common family context in which sibling dynamics are less understood is organized youth sport. The present dissertation was designed to address multiplegaps in the present literature. This dissertation is comprised of two complementary studies. Study 1, guided …

An Assessment Tool To Analyze Code Written In App Inventor, Rohit Gopalan

All Graduate Plan B and other Reports, Spring 1920 to Spring 2023

Assessment plays a significant role in today’s educational field. When creating an assessment tool, we should keep in mind that the tool measures learning, supports learning and encourages learning. Many assessment tools are available in the educational market such as Dr. Scratch, FCS1 Assessment Instrument, and Beyond the rubric. These tools help teachers to evaluate students’ performance in programming activities, but they do not completely measure the creativity shown by students in programming. For example, Dr. Scratch measures abstraction, problem decomposition, and parallelism. FCS1 Assessment Instrument measures a set of foundational computer science concepts like variables, loops, and arrays. It …

An Examination Of Entrainment In Typically Developing Children, Kiersten A. Pope

All Graduate Plan B and other Reports, Spring 1920 to Spring 2023

Purpose: Conversational entrainment describes the tendency for individuals to align their behavior with their communication partners and is essential for successful interaction. Evidence of entrainment in adults is robust, yet research regarding its development is sparse. Here, we investigate the effectiveness of a quasi-conversational paradigm for the purpose of identifying the speech rate entrainment abilities of children.

Method: Data were collected from a total of 50 typically developing children from 5-14 years old. Participants completed an entrainment task to identify the presence of speech rate modification depending on the presence of “fast” or “slow” stimuli. The entrainment task utilized a …

Low Temperature Desiccants In Atmospheric Water Generation., Sunil Gupta

Electronic Theses and Dissertations

Surging global water demand as well as changes to weather patterns and over exploitation of natural water sources, such as ground water, has made potable water a critical resource in many parts of the World already – one rapidly heading towards a crisis situation. Desalination has been adopted as a solution – this is however energy intensive and impractical for most of the developing countries - those most in need of water. A renewable source of energy is solar thermal and solar photovoltaic. A plentiful source of water is the humidity in the atmosphere. This research is to push the …

Role Of Trkb-Signaling In Taste Development And Function., Jennifer Rios-Pilier

Electronic Theses and Dissertations

In the geniculate ganglion, taste neurons likely differentiate into subtypes during development, but very little is known about how these neurons are defined molecularly or how they differentiate. Embryonically, geniculate neuron development is regulated by the growth factor, brain ­derived neurotrophic factor (BDNF). Postnatally, BDNF becomes restricted to subpopulations of taste receptor cells with specific functions, primarily sour responding. I hypothesized that during development, the receptor for BDNF, tropomyosin kinase B receptor (TrkB), also becomes restricted to a neuronal subset. I used transgenic mouse models to label and quantify both geniculate sensory neurons (Phox2b+), and those expressing TrkB (GFP) across …

Particulate Hexavalent Chromium Does Not Induce Centrosome Amplification In Sperm Whale And Bowhead Whale Cells., Jennifer Haruka Toyoda

Electronic Theses and Dissertations

Hexavalent chromium [Cr(VI)] is a widespread environmental and occupational carcinogen. The mechanism of carcinogenesis remains poorly understood, but chromosome instability (CIN) is the dominant theory. How numerical CIN arises is unclear, but it correlates with centrosome amplification. Both phenotypes are hallmarks of cancers, early events in carcinogenesis, and have been shown to occur after Cr(VI) exposure to human skin and lung fibroblasts. In this study, we investigate numerical CIN and centrosome amplification in whale cells. Whales are our closest marine relatives, have long lifespans, and are exposed to environmental Cr(VI). Importantly, they have low cancer rates and cell culture studies …

The Predictive Ability Of Self-Control And Differential Association On Sports Fans’ Decision To Engage In Cyber Bullying Perpetration Against Rivals, Seyyed Masoomeh S. Shadmanfaat, Christian Jordan-Michael Howell, Caitlyn N. Muniz, John K. Cochran, Saeed Kabiri, Dustin A. Richardson

Criminology Faculty Publications

A sample of 318 students from two Iranian universities was employed to conduct a test of Gottfredson and Hirschi’s self-control theory and elements of Akers’ social learning theory. Specifically, we sought to determine whether these theories are capable of explaining the decision to cyber bully members and fans of rival sports teams. Structural equation modeling was used to analyze these data. Findings suggest that individuals with low levels of self-control are more likely to engage in cyber bullying perpetration. Similarly, those who associate with delinquent peers are more likely to engage in cyber bullying perpetration. Additionally, we found that ineffective …

Statistical Methods To Account For Gene-Level Covariates In Normalization Of High-Dimensional Read-Count Data, Lauren Holt Lenz

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The goal of genetic-based cancer research is often to identify which genes behave differently in cancerous and healthy tissue. This difference in behavior, referred to as differential expression, may lead researchers to more targeted preventative care and treatment. One way to measure the expression of genes is though a process called RNA-Seq, that takes physical tissue samples and maps gene products and fragments in the sample back to the gene that created it, resulting in a large read-count matrix with genes in the rows and a column for each sample. The read-counts for tumor and normal samples are then compared …

Non-Contact Evaluation Methods For Infrastructure Condition Assessment, Sattar Dorafshan

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The United States infrastructure, e.g. roads and bridges, are in a critical condition. Inspection, monitoring, and maintenance of these infrastructure in the traditional manner can be expensive, dangerous, time-consuming, and tied to human judgment (the inspector). Non-contact methods can help overcoming these challenges. In this dissertation two aspects of non-contact methods are explored: inspections using unmanned aerial systems (UASs), and conditions assessment using image processing and machine learning techniques. This presents a set of investigations to determine a guideline for remote autonomous bridge inspections.

Riding To Learn: Informal Science In Adult Cycling Communities, Joel R. Drake

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Our understanding of how the world works is shaped through countless interactions with things in it. These interactions are our first exposure to science. Through them, we learn that heavy things are hard to push and books do not fall through tables. Our interactions are also shaped by the rules of the groups to which we belong (e.g., families, religious organizations, athletic teams). These rules lead us to accept that some things cannot or should not be done, limiting our interactions with the world. At the same time, these rules change our appreciation for what we do experience.

Prior research …

The Role Of Cathelicidin In The Regulation Of Gut Microbiota And Inflammasome Activation In Alcoholic Liver Disease., Fengyuan Li

Electronic Theses and Dissertations

Alcoholic liver disease (ALD) is characterized by gut dysbiosis and hepatic and intestinal inflammation. Gut microbiota is critically maintained by antimicrobial peptides (AMPs) expressed in the epithelial and immune cells. AMPs are also identified as immune-regulators in various diseases. Previous studies demonstrated that deficiency of AMPs REG3 lectins exacerbated alcoholic steatohepatitis. LL-37 is the sole member of human cathelicidin AMP family, named CRAMP in mouse. Despite of the extensive studies in infectious diseases and in gut-microbiota-related inflammatory bowel disease, the role of LL-37/CRAMP in ALD is yet unknown. Our previous studies showed a decreased intestinal CRAMP in a mouse model …

Larval Food Limitation In A Speyeria Butterfly (Nymphalidae): How Many Butterflies Can Be Supported?, Ryan I. Hill, Cassidi E. Rush, John Mayberry

College of the Pacific Faculty Articles

For herbivorous insects the importance of larval food plants is obvious, yet the role of host abundance and density in conservation are relatively understudied. Populations of Speyeria butterflies across North America have declined and Speyeria adiaste is an imperiled species endemic to the southern California Coast Ranges. In this paper, we study the link between the food plant Viola purpurea quercetorum and abundance of its herbivore Speyeria adiaste clemencei to better understand the butterfly’s decline and aid in restoration of this and other Speyeria species. To assess the degree to which the larval food plant limits adult abundance of S. …

Assessing Ground Penetrating Radar's Ability To Image Subsurface Characteristics Of Icy Debris Fans In Alaska And New Zealand, Robert W. Jacob, Jeffrey M. Trop, R. Craig Kochel

Faculty Journal Articles

Icy debris fans have recently been described as fan shaped depositional landforms associated with (or formed during) deglaciation, however, the subsurface characteristics remain essentially undocumented. We used ground penetrating radar (GPR) to non-invasively investigate the subsurface characteristics of icy debris fans (IDFs) at McCarthy Glacier, Alaska, USA and at La Perouse Glacier, South Island of New Zealand. IDFs are largely unexplored paraglacial landforms in deglaciating alpine regions at the mouths of bedrock catchments between valley glaciers and icecaps. IDFs receive deposits of mainly ice and minor lithic material through different mass-flow processes, chiefly ice avalanche and to a lesser extent …

Functional Consequence Of Psat1 Association On Pkm2'S Inherent Enzymatic Activity., Alexis Avidan Vega

Electronic Theses and Dissertations

Pyruvate kinase M2 (PKM2) is predominantly found in tumors, where it allows the cancer cell to adapt to metabolic conditions through allosteric regulation of its activity. We recently discovered that phosphoserine aminotransferase 1 (PSAT1) associates with and activates PKM2. Here, I sought to affirm PSAT1's ability to increase PKM2 activity through kinetic and association studies of wild-type or mutant PKM2 enzymes. I demonstrate that His-tagged WT and mutant PKM2 enzymes are active, exhibit different kinetics, yet cannot be activated by PSAT1. Comparison studies using untagged WT PKM2 suggest that inclusion of the His-tag disrupts PSAT1 association. In support, pull-down strategies …

Lead And Crime: An Ecological Study Between Lead Contaminated Topsoil And Violent Crime., Brian Guinn

Electronic Theses and Dissertations

Lead is a known neurotoxicant. Human exposure to lead comes primarily through environmental exposures, including remnant lead paint, lead contaminated topsoil and lead contaminated water. Prenatal and early life lead exposure has been associated with numerous neurocognitive and neuropsychiatric disorders. This dissertation presents findings from an ecological study which evaluated the geospatial association between topsoil lead content and the incidence of FBI designated violent crime in Jefferson County, Kentucky. A total of 412 topsoil samples were collected along roadways (n=300) and from Louisville Metro Parks (n=112). Jefferson County crime data was obtained from the Louisville Metro Police Department – Crime …

Impacts Of Ammonia And Temperature On Freshwater Snail Behavior And Physiology., Megan Christine Dewhatley

Electronic Theses and Dissertations

Gastropods are one of the most imperiled groups of animals in North America, yet there are major gaps in the literature pertaining to pollutants and climate change, and especially sublethal impacts. This dissertation assesses the effects of climate warming and unionized ammonia (NH₃), one of the most abundant water pollutants, on the behavior and physiology of two caenogastropod snails: fine-ridged elimia (Elimia semicarinata) and Shawnee rocksnails (Lithasia obovata) (Gastropoda: Neotaenioglossa: Pleuroceridae). Righting behavior, or the movement used by snails to turn themselves right-side-up, was used as the main endpoint; delays in this behavior compromise …

Is Macrolide And Beta-Lactam Combination Therapy Associated With Early Clinical Stability Or 30 Day Mortality In Hospitalized Patients With Pneumonia? An Assessment Of Confounding By Indication., Evangeline Pierce

Electronic Theses and Dissertations

Pneumonia and influenza are one of the leading causes of infectious disease-related deaths worldwide. Current guidelines for the treatment of hospitalized patients with community acquired pneumonia (CAP) include empiric antimicrobial therapy with a macrolide and a beta-lactam. There is little consensus among studies as to which antimicrobial regimen is best. The confusing results seen may very well be due to lack of assessment of confounding by indication (CBI). This analysis was a secondary analysis from Hospitalized Adults with Pneumococcal Pneumonia: Incidence Study (HAPPI). The study participants were those in HAPPI who had received either macrolide and beta-lactam combination therapy or …

Missouri Meat Goat Producers: A Focus Group Approach To Identify Opportunities And Challenges, Samantha N. Riley

Graduate Theses/Dissertations

This study was conducted to better understand opportunities and challenges for Missouri meat goat producers. The meat goat industry is expanding and is one of the fastest growing segments of livestock production in the United States, both in inventory and markets for products produced from goats. This study used focus groups as a way to determine what a sampling of producers in Missouri see as opportunities and challenges based on themes and patterns found in the focus groups. The participants in the three focus groups did not always agree with the literature, but they did see the following as opportunities …

Comparative Study Of U.S. And E.U. Experiences With Policies On Immigration Controls, Daniel Brady

Graduate Theses/Dissertations

The year 2015 marked a colossal change in how the world views immigration. The migration crisis of Europe and the emergence of President Donald Trump continue to have lasting impacts. Major political, demographic, and social conflicts are playing out in the U.S. and Europe over migration. In the U.S., President Trump has been able to enact anti-immigration sentiment into policy. Contrary to that, in Europe, the 28 member states of the European Union have failed to come up with a clear, unifying plan to deal with the migration crisis. The consequences of migration will be harsher and less manageable in …

Novel Regulatory Pathways Of Protein Channels, Sheenah Lynn Bryant

Boise State University Theses and Dissertations

Since the proposal of the fluid mosaic model of a cell membrane, substantial scientific evidence has shown that the cell membrane is not simply an inert structure with the sole role of separating two chemically different environments. The cell membrane dynamically satisfies basic needs, such as water, ion and nutrient transport, without which the cell could not survive. It is a structure which actively participates in a great variety of physiological functions. The activity of the cell membrane is responsible for the contraction of our muscles and information processing in our brain. In order to participate in such a wide …

Knowledge Management & Data Literacy, Sony Shah

Books, Presentations, Posters, Etc.

Using Knowledge Management and Data Literacy to enable caregivers to have ready access to Organizations’ documented base of facts, source of information and solutions.