Digital Commons Network^™

Rare But Recurrent Ros1 Fusions Resulting From Chromosome 6q22 Microdeletions Are Targetable Oncogenes In Glioma., Monika A Davare, Jacob J Henderson, Anupriya Agarwal, Jacob P Wagner, Sudarshan R Iyer, Nameeta Shah, Randy Woltjer, Romel Somwar, Stephen W Gilheeney, Ana Decarvalo, Tom Mikkelson, Erwin G Van Meir, Marc Ladanyi, Brian J Druker

Articles, Abstracts, and Reports

PURPOSE: Gliomas, a genetically heterogeneous group of primary central nervous system tumors, continue to pose a significant clinical challenge. Discovery of chromosomal rearrangements involving kinase genes has enabled precision therapy, and improved outcomes in several malignancies.

EXPERIMENTAL DESIGN: Positing that similar benefit could be accomplished for patients with brain cancer, we evaluated The Cancer Genome Atlas (TCGA) glioblastoma dataset. Functional validation of the oncogenic potential and inhibitory sensitivity of discovered ROS1 fusions was performed using three independent cell-based model systems, and an

RESULTS:

CONCLUSIONS: Our findings highlight that CNS tumors should be specifically interrogated for these rare intrachromosomal 6q22 microdeletion …

Palynology Of The Cenomanian Woodbine Group, Marlee Cloos

Earth Sciences Theses and Dissertations

This study explores the palynology of the Cenomanian Woodbine Group in order to answer questions about the paleoecology of North Texas during the Cenomanian and how palynofloras vary at different localities with different facies, as well as the radiation of angiosperms in the Late Cretaceous. This study also helps our understanding of the paleoecology after the Albian Trinity Group in Texas. Three localities in North Texas were sampled and a total of seventeen productive samples were analyzed for palynological data. Pollen was extracted from mudstones from two depositional environments: lower delta plain marshes and a meandering river system. The palynomorphs …

2018 Fall - Commencement Program, Office Of The Registrar, East Tennessee State University

ETSU Commencement Ceremonies and Programs

No abstract provided.

Fundraising Managers’ Perceptions Of Online Education’S Influence On Fundraising Practices And Giving, Peter J. Szucs

CUP Ed.D. Dissertations

The influence of online education on higher education fundraising practices and giving patterns is not well understood. This qualitative case study explored how fundraising managers perceived the impact of online education on higher education fundraising. To explore this phenomenon, the researcher interviewed fundraising managers at private, nonprofit colleges and universities in the western United States, and reviewed publicly available documents on the participant schools fundraising and financial information. The interviews demonstrated that study participants were concerned about connecting with graduates of online programs, and perceived that online students may have less affinity for their alma mater than on-ground alumni. The …

Arts Integration In The Public Elementary School General Education Classroom, Norma Quinones

Theatre & Dance ETDs

ABSTRACT

In 2017, the U.S. Department of Education’s Nations Report Card disclosed performance trends among fourth and eighth grade students in New Mexico. Fourth graders were significantly below the national average in math and reading, while eighth graders were also performing below the national average (U.S. Department of Education). How can New Mexico public elementary school students fulfill their learning potential? Extensive research shows that arts integrated instruction is associated with student achievement. It outlines arts integration as a multidisciplinary form of teaching across the curriculum to transform teaching and learning at the elementary school level to change such statistics. …

Spatial Exploration Of Trace Metals In Near-Surface Water And Soil In The Bolivian Altiplano, Lizzie Wilson

Civil and Environmental Engineering Theses and Dissertations

We analyzed soil and groundwater samples from the Bolivian Altiplano for trace metal concentrations, correlations and partitioning trends. Total digestion, sequential extraction and loss on ignition experiments were performed on soil samples. We ran aqueous samples on an ICP-MS. Results were analyzed to identify trace metal concentrations and find any significant correlations. We generated interpolated maps in ArcGIS to spatially analyze results. Results for elevated concentrations of interest were divided into sub-groups of organophilic metals, oxoanions and divalent metals and include vanadium, arsenic, selenium, zinc, nickel, lead, cobalt, uranium, copper and manganese. These elements are likely naturally sourced. We found …

Racial Integration And White Supremacy In America: Can America Integrate Its Power Structures And Liberate Them From White Supremacy, Billy Lane

Graduate Liberal Studies Theses and Dissertations

Neither the presence of black people in predominantly white spaces nor the appropriation of black culture are indicators of racial justice. Power structures must be integrated even more so than subdivisions, classrooms, breakrooms, church pews, and pop culture. This thesis will explore the absence of African-American leadership from the highest ranks of our power structures that are central to contemporary life and the dynamics within each of these power structures that help to protect white supremacy and therefore maintain segregation.

Resistance Beyond The Frontier: Concepts And Policies For The Protection Of Isolated Indigenous Peoples Of The Amazon, Minna Opas, Luis Felipe Torres, Felipe Milanez, Glenn Shepard Jr.

Tipití: Journal of the Society for the Anthropology of Lowland South America

No abstract provided.

The Father Of Modern Constitutional Liberalism, John Lawrence Hill

William & Mary Bill of Rights Journal

No abstract provided.

Between Developed And Emerging Markets: Globalization Of Large Consumer Good Enterprises, Christine Savino Ms.

Honors Scholar Theses

A few years ago, The Economist promulgated that “the only way that companies can prosper in [emerging] markets is to cut costs relentlessly and accept profit margins close to zero” (The Economist, 2010). Likewise, according to the World Bank’s 2018 Ease of Doing Business Index[1], the least ranked countries (#94 and under out of 190 countries) are solely undeveloped and developing, while the top 20 countries are solely developed economies (World Bank, 2018). It has been long held that emerging markets[2][3] are non-strategic investments for multinational expansion, and that business giants from …

Nanopower Analog Frontends For Cyber-Physical Systems, Kenji Aono

McKelvey School of Engineering Graduate Student Theses & Dissertations

In a world that is increasingly dominated by advances made in digital systems, this work will explore the exploiting of naturally occurring physical phenomena to pave the way towards a self-powered sensor for Cyber-Physical Systems (CPS). In general, a sensor frontend can be broken up into a handful of basic stages: transduction, filtering, energy conversion, measurement, and interfacing. One analog artifact that was investigated for filtering was the physical phenomenon of hysteresis induced in current-mode biquads driven near or at their saturation limit. Known as jump resonance, this analog construct facilitates a higher quality factor to be brought about without …

Combining Footprinting Mass Spectrometry And Molecular Dynamics Simulation For Structural Studies In Membrane Proteins, Fengbo Zhou

Arts & Sciences Graduate Student Theses and Dissertations

Membrane proteins are essential in many cellular processes and represent ~60% of all current drug targets. Due to technical limits, membrane proteins of various types were not studied extensively in the past and the biochemistry and functionality of them remain unclear. The structural biology methodologies require pure isolated protein samples for us to resolve their structure and study their biochemical functions. For such in vitro studies, however, membrane proteins often become unstable when isolated from their native lipid bilayer environment. To overcome the challenge, I employed a novel methodology of solubilizing membrane proteins in solution without detergent. I reconstituted ferroportin, …

The Essential Roles Of The Chromatin Factor Gon4l In Heart Development, Terin Elise Budine

Arts & Sciences Graduate Student Theses and Dissertations

Heart development and the genetic pathways underlying it are highly conserved among vertebrates. During heart development, an embryo must induce mesoderm formation, pattern the mesoderm, specify cardiomyocytes, increase the population of cardiomyocytes through proliferation, and pattern the cardiac chambers. It is becoming increasingly clear that chromatin modifications help mediate the complex processes of heart development by providing spatiotemporal regulation of gene expression. My thesis work focuses on characterizing functions of the chromatin factor Gonad-4-like (Gon4l), encoded by the gene ugly duckling (udu), in zebrafish heart development. Previous works established a requirement for Gon4l in the formation of many mesoderm derivatives …

Modeling The Bioactivation And Subsequent Reactivity Of Drugs, Tyler Brian Hughes

Arts & Sciences Graduate Student Theses and Dissertations

Metabolism can convert drugs to harmful reactive metabolites that conjugate to DNA and off-target proteins. Reactive metabolites are a significant driver of both drug candidate attrition and withdrawal from the market of already approved drugs. Unfortunately, reactive metabolites are difficult to study in vivo, because they are transitory and generally do not circulate. Instead, this work computationally models both metabolism and reactivity. Using deep learning, predictive models were developed for the metabolic formation of quinones and epoxides, which together account for about half of known reactive metabolites. Additionally, an accurate model of DNA and protein reactivity was constructed, which predicts …

A Mouse Model Of Börjeson-Forssman-Lehmann Syndrome Reveals A Potential Link With Autism Spectrum Disorder, Cheng Cheng

Arts & Sciences Graduate Student Theses and Dissertations

ABSTRACT OF THE DISSERTATIONA Mouse Model of Börjeson-Forssman-Lehmann Syndrome reveals a potential link with Autism Spectrum DisorderByCheng ChengDoctor of Philosophy in Biology and Biomedical SciencesDevelopmental, Regenerative and Stem Cell BiologyWashington University in St. Louis, 2018Dr. Azad Bonni, ChairIntellectual disability (ID) is a prevalent neurodevelopmental disorder that affects 1% to 3% of the general population. ID is characterized by developmental deficiencies in cognitive function and adaptive behaviors. Lacking effective treatments, ID currently presents an immense burden to affected families and the economy. Therefore, there is an urgent need to elucidate the pathogenesis of ID. Human genetic studies have associated ID with …

Genetic Basis Of Thermal Divergence In Saccharomyces Species, Xueying C. Li

Arts & Sciences Graduate Student Theses and Dissertations

The genetic architecture of phenotypic divergence is a central question in evolutionary biology. Genetic architecture is impacted by whether evolution occurs through accumulation of many small-effect or a few large-effect changes, the relative contribution of coding and cis-regulatory changes, and the prevalence of epistatic effects. Our empirical understanding of the genetic basis of evolutionary change remains incomplete, largely because reproductive barriers limit genetic analysis to those phenotypes that distinguish closely related species. In this dissertation, I use hybrid genetic analysis to examine the basis of thermal divergence between two post-zygotically isolated species, Saccharomyces cerevisiae and S. uvarum. S. cerevisiae is …

A Recombinant Virus And Reporter Mouse System To Study Chronic Chikungunya Virus Pathogenesis, Alissa Roxanne Young

Arts & Sciences Graduate Student Theses and Dissertations

Chikungunya virus (CHIKV) is an arthritogenic alphavirus that during acute disease causes fever as well as severe joint and muscle pain. Chronic joint and muscle pain persists in a significant subset of patients, yet we still have a poor understanding of what drives this chronic disease. While replicating virus has not been detected in the joints of patients with chronic arthritis or in various animal models at chronic time points, persistent viral RNA can be detected for months after acute infection.To identify the cells that could be contributing to chronic CHIKV pathogenesis, we developed recombinant viruses that express Cre recombinase …

The Role Of Apolipoprotein E In Regulating Tau Pathogenesis And Neurodegeneration In A Tauopathy Mouse Model, Yang Shi

Arts & Sciences Graduate Student Theses and Dissertations

APOE4 is the strongest genetic risk factor for late-onset Alzheimer's disease (AD). APOE4 increases brain amyloid-β (Aβ) pathology relative to other APOE isoforms. However, whether APOE independently influences tau pathology, the other pathological hallmark of AD and other tauopathies, or tau-mediated neurodegeneration, is not clear. By generating P301S tau transgenic mice on either a human APOE knock in (KI) or APOE knockout (KO) background, we show that the presence of human APOE, regardless of APOE isoforms, leads to various degrees of brain atrophy in 9-month old P301S mice, whereas APOE ablation strongly protects against neurodegeneration. In particular, P301S/E4 mice develop …

Fast Objective Coupled Planar Illumination Microscopy, Cody Jonathan Greer

Arts & Sciences Graduate Student Theses and Dissertations

Among optical imaging techniques light sheet fluorescence microscopy stands out as one of the most attractive for capturing high-speed biological dynamics unfolding in three dimensions. The technique is potentially millions of times faster than point-scanning techniques such as two-photon microscopy. This potential is especially poignant for neuroscience applications due to the fact that interactions between neurons transpire over mere milliseconds within tissue volumes spanning hundreds of cubic microns. However current-generation light sheet microscopes are limited by volume scanning rate and/or camera frame rate. We begin by reviewing the optical principles underlying light sheet fluorescence microscopy and the origin of these …

Neural Mechanisms Of Drosophila Circadian Rhythms, Xitong Liang

Arts & Sciences Graduate Student Theses and Dissertations

Animals show circadian rhythms in a variety of physiological functions and behaviors. In Drosophila melanogaster, behavioral rhythms are driven by circadian clock genes that are oscillating in ~150 circadian pacemaker neurons. To explain how circadian neurons encode time and regulate different behavioral rhythms, I performed 24-hour in vivo whole-brain calcium imaging using light-sheet microscopy. First, I found that different groups of circadian neurons show circadian rhythms in spontaneous neural activity with diverse phases. The neural activity phases of the M and E pacemaker groups, which are associated with the morning and evening locomotor activities respectively, occur ~4 hours before their …

The Effect Of Talker And Contextual Variability On Memory For Words In Sentences, Nichole Runge

Arts & Sciences Graduate Student Theses and Dissertations

Previous research has found that adding different forms of variability during study can affect later memory at test. For example, having words spoken by different talkers has been shown to improve recall of known and novel words (Goldinger et al., 1999; Barcroft & Sommers, 2005), and varying the cues in cue-target related word pairs has been found to improve recall of the targets (Glenberg, 1979; Bevan et al., 1966). It was unclear, however, whether benefits of variability would extend to more naturalistic stimuli, such as sentences, which have higher working memory demands. The present set of experiments investigated how talker …

Corporate Social Responsibility To-Come: A Derridean Interruption Of Transparency, Robert Foschia

Electronic Theses and Dissertations

This study investigated the relation between rhetorics of transparency and organizational action. Digging into CSR literature from a philosophy of communication perspective, this project seeks to determine if corporate social responsibility delivers on the promises it makes of a better world.

Drawing on the work of Jacques Derrida, the research first lays out his often overlooked contribution to the philosophy of communication, and then moves towards possible applications in deconstructing the perceived benefits of CSR, particularly in its transparent nature. By looking at organizational life from the Triple Bottom Line, this dissertation peels back the underlying rhetoric of planet, people, …

"Who Are These Wild Indians": On The Foreign Policies Of Some Voluntarily Isolated Peoples In Amazonia, Peter Gow

Tipití: Journal of the Society for the Anthropology of Lowland South America

This paper is a reflection on the phenomenon of voluntary isolation in Amazonia, about anthropology’s implication in its formation as a concept, and what anthropologists might profitably say about it as a concrete phenomenon in the world. While knowledge based on ethnographic fieldwork might by minimal or even totally absent for people in voluntary isolation, anthropological research has produced a very impressive understanding of indigenous Amazonian social forms in general, knowledge that can be brought to bear on the question.

Werken By Bernardo Oyarzún (Mapuche), Ana Guevara, Sophie Moiroux

Tipití: Journal of the Society for the Anthropology of Lowland South America

No abstract provided.

Good Reasons Or Bad Conscience: A Postscript, Stephen P. Hugh-Jones

Tipití: Journal of the Society for the Anthropology of Lowland South America

Published in French in 1996, the original article for which this comprises a post-script set indigenous Amazonians’ attitudes to meat alongside those of Euro-Americans. With the accelerating deforestation of Amazonia linked with the cultivation of soya used to feed animals for meat, and with calls to reduce or abandon meat consumption as one way of averting catastrophic climate change, it is topical once again. In this postscript, I reply to two contrasting critiques of the article, the first wary of an excess of ontology, the second distrustful of a deficit of it. Does a focus on ritual and shamanism obscure …

Making Space For Makerspace: How Adding A Makerspace Can Benefit Art Museum Education Programming, Kaeleigh Thorp

Master's Projects and Capstones

In this capstone project, I advocate for adding makerspaces to art museum education programming. I review the foundational educational concepts of a makerspace which help explain why it is a modern strategy to increasing visitor learning. This includes John Dewey’s theories of experience and education, Howard Gardner’s multiple intelligences theory, George Hein’s studies of museum visitor behavior, and the physiological science of learning. I then propose four key terms essential for visitor learning, and explain how makerspaces successfully incorporate them. The four key terms are: 1. multiple intelligences, 2. direct experience, 3. inquiry based and open ended learning, and 4. …

Expanding The Definition Of Provenance: Adapting To Changes Since The Publication Of The First Aam Guide To Provenance Research, Katlin Cunningham

Master's Projects and Capstones

In 2001 the American Alliance of Museums (AAM) published The AAM Guide to Provenance Research. However, in the past seventeen years there have been several new developments in the provenanceresearchfield, and an updated guide has yet to be released. I propose that several changes be made to create an updated version. For this project, I tested this guide in two ways: first, to see how our understanding of provenance has changed since 2001 and second, to use a case study to assess whether the recommended basic research guide and principles still apply to today.

In the first …

Why Musical Groove Makes Us Move: An Electroencephalographic Investigation, Samantha Reina O'Connell

UNLV Theses, Dissertations, Professional Papers, and Capstones

Moving to the groove of the music is a phenomenal and universal human behavior. Common characteristics to most dance music include a salient beat, rhythmic complexity, and a dynamic musical structure. What is unknown, however, is why music has the power to promote physical movement. Musical rhythm and motor processing are tightly linked activating brain areas important for motor preparation and execution. While studies demonstrate that the motor system is active during beat processing, it is still unclear how musical groove, a beat-influenced quality to music that makes us want to move, can engage these motor areas. Here, I performed …

The Use Of Chemical Element Fingerprints To Investigate The Transformation Of Lake Sediments To Land Soils In Drying Lakes: A Case Study At Lake Mead, Nevada, Ji Hye Park

UNLV Theses, Dissertations, Professional Papers, and Capstones

Drying lakes are a worldwide catastrophe. The goals of this dissertation research were (1) to investigate whether chemical fingerprints of lake sediments, and nearby land soils, could be established by the analysis of 44 chemical elements, moisture content, Eh, pH, and leachable anions and if so, (2) whether the fingerprints could help explain the process of physical and chemical changes in the lake sediments as they became soils when the lake's water level fell and the anoxic sediments were exposed to the air and weather.

Lake Mead was chosen to model drying lakes because its water level has fallen over …

Prokaryotic Diversity And Aqueous Geochemistry Of Subsurface Environments Of The Death Valley Regional Flow System, Joshua David Sackett

UNLV Theses, Dissertations, Professional Papers, and Capstones

This dissertation summarizes over four years of effort towards the completion of a Ph.D. in Biological Sciences. The work presented in this document covers a broad range of topics, but a central unifying theme is prokaryotic life in the continental subsurface. The work presented in each chapter relied heavily on cultivation-independent methods for assessing prokaryotic communities, including prokaryotic community structure reconstruction from high-throughput sequencing of 16S rRNA gene libraries and single-cell genome analysis of novel uncultivated bacteria.

Chapter 2 examines the aqueous geochemistry and prokaryotic diversity of Devils Hole, a cavernous limnocrene and sole natural habitat for the critically endangered …