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An Agile Quality Management System For Laboratory Developed Tests, Rita D'Angelo

Wayne State University Dissertations

ABSTRACT

AN AGILE QUALITY MANAGEMENT SYSTEM FOR LABORATORY DEVELOPED TESTS

By

RITA D’ANGELO

MAY 2017

Advisor: Dr. Ratna Babu Chinnan

Major: Industrial & Systems Engineering

Degree: Doctor of Philosophy Statement of the Problem: We explore the 2014 draft guidance by the FDA entitled Framework for Regulatory Oversight of Laboratory Developed Tests (LDT) extended from the medical device industry and discuss how these requirements may be applicable to laboratory medicine. We introduce terms, definitions and provide a call for action for leaders to prepare for the potential adherence to regulatory requirements and explore if compliance was achievable in a laboratory environment …

The Impact Of Neonatal Pain And Reduced Maternal Care On Brain And Behavioral Development, Sean Michael Mooney-Leber

Wayne State University Dissertations

In the neonatal intensive care unit (NICU) preterm infants are exposed to a multitude of stressors, which include both neonatal pain and reduced maternal care. Clinical and preclinical research has demonstrated that exposure to neonatal pain and reduced maternal care has a profound negative impact on brain and behavioral development. Currently, the biological mechanism by which both of these stressors impacts brain and behavioral outcomes remains widely unknown. To uncover a potential biological mechanism, the current dissertation project utilized a preclinical model of repetitive needle pokes and developed a novel model of reduced maternal care through tea-ball encapsulation. Briefly, rat …

Orthorexia Nervosa In Undergraduate Health Majors: Using Yoga As An Intervention, Ashley J. Kyle

Graduate Research Theses & Dissertations

Purpose and Objectives: While much is known about common eating disorders in undergraduate students in college, limited research is available on the prevalence of orthorexia nervosa (ON). Orthorexia nervosa is a fixation on righteous eating that typically stems from an attempt to diet or consume healthier foods and turns into an obsession with the quality and purity of food. This type of behavior can become extremely restrictive, damage mental and physical health, and may lead disruptions in daily life. Specifically, information is lacking on the prevalence of ON in health majors, how to classify this disorder, and how to treat …

Biochemical And Cellular Studies Of Apobec3 Family Dna-Cytosine Deaminases, Sachini Umedi Siriwardena

Wayne State University Dissertations

The AID/APOBEC family of enzymes deaminate cytosines in single-stranded DNA to uracils leading to base substitutions and strand breaks. Members of APOBEC3 family in humans are induced by cytokines produced during the body's inflammatory response to infections and provide innate immunity against viruses. However, there is emerging consensus that these enzymes can cause mutations in the cellular genome depending on the physiological state of the cell and the phase of the cell cycle they are expressed. Since aberrant expression of APOBEC3B was recently identified as a possible source of cancer, we initiated a study to determine the maximally active catalytic …

Toward Better Keys For Lectin Locks: Glycoconjugates Versus Glycomimetics, Peng Wen

Wayne State University Dissertations

As one of the four major classes of macromolecule in living systems, the roles of carbohydrates as energy storage substances and structural components have long been recognized. However, the unique role of carbohydrates in cellular recognition processes has only been scrutinized since the 1960s, thanks to the progress in the research in both cell surface carbohydrates and carbohydrate-binding proteins (lectins). The advances in the research of such process demand the use of carbohydrates as basic tools. Therefore, the primary goal of glycochemistry has been to obtain complex carbohydrates and their analogs effectively. One common approach to the problem involves the …

The Life History Of Faxonius Rusticus (Girard, 1852) In Sunfish Creek, Monroe County, Ohio, Kyle Mcgill

Theses, Dissertations and Capstones

Crayfish are among the most imperiled faunal groups globally. The continued spread of invasive species is a major impact to crayfish. Invasive species, much like the native species, are often understudied. As declines continue, invasive species spread, and new species are discovered; additional life history studies are as important as ever for crayfish conservation. Life history data was collected for rusty crayfish (Faxonius rusticus) from October 2017 to September 2018 in Sunfish Creek, Monroe County, Ohio. Faxonius rusticus are native to the Ohio River drainage around Cincinnati, Ohio but have extensively expanded their range over the past 30 years. Sunfish …

Multiple Signaling Functions Of Song In A Polymorphic Species With Alternative Reproductive Strategies, M. L. Grunst, A. S. Grunst, Vincent A. Formica, R. A. Gonser, E. M. Tuttle

Biology Faculty Works

Vocal traits can be sexually selected to reflect male quality, but may also evolve to serve additional signaling functions. We used a long-term dataset to examine the signaling potential of song in dimorphic white-throated sparrows (Zonotrichia albicollis). We investigated whether song conveys multifaceted information about the vocalizing individual, including fitness, species identity, individual identity, and morph. We also evaluated whether song traits correlate differently with fitness in the two morphs, as the more promiscuous strategy of white, relative to tan, morph males might impose stronger sexual selection. Males with high song rates achieved higher lifetime reproductive success, and this pattern …

Rethinking Parts And Wholes, Scott F. Gilbert

Biology Faculty Works

No abstract provided.

Evaluating The Tosrec As A Brief Rti Screen For Early Struggling Readers In Urban Schools, Cheryl C. Durwin, Dina Moore, Deborah A. Carroll

NERA Conference Proceedings 2018

Efficient response-to-intervention (RTI) screening is critical in urban schools where 60-80% of students read below grade-level. We evaluated the classification validity of the Test of Silent Reading Efficiency and Comprehension (TOSREC) in identifying students receiving school services using two first-grade cohorts in the same urban school. TOSREC slightly under-identified students receiving school interventions; however, most “under-identified” students did not receive interventions for reading problems. TOSREC accurately identified those not-at-risk, but over-identified students who were receiving our research-based reading intervention while waiting for school services. Results suggest that schools need sufficient resources to provide appropriate services for all students needing intervention.

A Literature Review Of The 3cam Model: A Pathway To Apply Critical Thinking And Mastery In A Classroom, Elham Zandvakili, Ernest Washington

NERA Conference Proceedings 2018

This review of the literature starts with the theoretical framework and culminates in a picture of the 3CAM model. The literature review focuses upon the relevant literature for each of the components of the 3CAM model: mastery learning, concept maps, critical thinking, collaboration and assessment. The literature review will come to an end with a review of the 3CAM model.

Efficacy Of A Dialogic Reading Intervention For Struggling First-Graders In Urban Schools, Dina Moore, Cheryl C. Durwin, Deborah A. Carroll

NERA Conference Proceedings 2018

The present study investigates the efficacy of dialogic reading (DR) intervention to improve reading comprehension with first-grade cohorts from two school years within one urban school. We adapted DR, a shared book reading technique, using a standard set of books for intervention and added an emphasis on vocabulary. Findings replicated our previous research in which DR intervention reduced the reading comprehension gap between at-risk readers and typically-achieving peers with a total intervention time of about 2 hours over 12 weeks. These results suggest that our intervention is a promising technique for struggling readers.

'The Good Shepherd Lays Down His Life For The Sheep' (John 10:11, 15, 17): Questioning The Limits Of A Johannine Metaphor, Christopher W. Skinner

Theology: Faculty Publications and Other Works

Scholars have long recognized that the Good Shepherd discourse reflects a realistic picture of the ancient Palestinian shepherd and his relationship to the flock. But how far should the details in this metaphor be pressed? It is often asserted that any halfway decent shepherd would have been willing to die on behalf of his flock and that Jesus’ statement to that effect (10:11, 15, 17) reflects a common expectation. This scenario, however, would actually leave a flock more exposed to danger. Thus, it is appropriate to understand Jesus’ pronouncement as falling outside the realm of historical verisimilitude and better to …

An Unfinished Project: John Courtney Murray, Religious Freedom, And Unresolved Tensions In Contemporary American Society, Miguel H. Diaz

Philosophy: Faculty Publications and Other Works

Religious freedom has re-emerged as a controversial issue in the courts, in the Church, and in the public square in the United States. This essay examines the groundbreaking contribution that John Courtney Murray, SJ made to bring about a paradigm shift in Roman Catholic teaching on religious freedom. This shift can be traced to the Church’s transitioning from the view that “error has no rights” to only people—not ideas—have rights. The essay underscores Murray’s focus on human conscience and addresses tensions that have emerged in the United States between voices that affirm the right to religious freedom and those that …

2017 Annual Report Of The Officers Town Of Orange, New Hampshire., Orange Town Representatives

Orange, NH Annual Reports

This is an annual report containing vital statistics for a town/city in the state of New Hampshire.

To Kill A Migratory Bird: How Incidental Takes By Commercial Industry Activity Should Be Regulated By A New Civil Penalty Regime, Not The Current Mbta, Brittany E. Barbee

Buffalo Environmental Law Journal

Migratory birds are at odds with commercial industries in the United States. Industries are occasionally and accidentally killing migratory birds through their legal activity. Such actions against migratory birds are known as incidental takes. While the century-old Migratory Bird Treaty Act (MBTA) prohibits the taking of migratory birds, it is unclear as to whether it prohibits these modern-day incidental takes. The MBTA imposes criminal strict liability on those who violate its prohibitions, regardless of one's mental state at the time of the incident. Should the federal government hold commercial industries criminally liable for incidentally taking migratory birds through otherwise legal …

Policy Meltdown: How Climate Change Is Driving Excessive Nuclear Energy Investment, Ashley Hardy, Dontan Hart

Buffalo Environmental Law Journal

The United States is currently experiencing what some have labeled a nuclear energy renaissance. This so-called renaissance responds in part to growing concerns about global warming and the need to reduce the greenhouse gas emissions associated with electricity production. A growing number of policymakers and scholars view nuclear energy development as one of the most promising means of slowing climate change because nuclear energy does not produce greenhouse gas emissions. They are increasingly advocating that nuclear energy receive policy treatment at least as favorable as that afforded to renewable energy strategies such as wind and solar energy. Some state governments …

Learning To Do In Vivo Neural Responses In Mice, Timothy Mogan, Justin Wobser, Riley Raulhammer, Tyler Erker

Undergraduate Research Posters 2018

Timothy Mogan, Tyler Erker, Riley Faulhammer and Justin Wobser were the target students for this stereotaxic neurosurgery and electrophysiology lab experience. They are Pre-Nursing, Pre-Neuroscience (Pyschology) or Pre-Med majors. This richly educational and hands-on investigation significantly enhanced their confidence and experience in RODENT HANDLING, ANESTHESIA, ELECTROPHSYIOLOGY, PERFUSION, BRAIN REMOVAL and GENERAL LAB SKILLS. An animal use protocol was created for the project under the guidance of the Mentors and the students followed it competently. Students completed CITI online animal research training and animal handling training was provided by Lou Turchyn, DVM. Animals for the research were generously donated by Dr. …

A Targeted Genetic Screen To Identify Meiotic Cohesin Regulators, Urja Patel, Emilia Kalutskaya, Alexis Brown, Ali Ahsan

Undergraduate Research Posters 2018

During oogenesis in animals deficient for REC-8, a cohesin subunit required for sister chromatids cohesin (SCC), Co recombination fails and sister chromatids segregate away from one another prematurely in meiosis I. Consequently, zygotes inherit two copies of each chromosome. Chromosome segregation in meiosis II fails and the progeny of rec-8 mutant mothers usually survive as viable polyploids. In contrast, homologs segregate randomly during meiosis I in oocytes produced by spo-11 mutants, which lack the transesterase required for crossover recombination. This results in aneuploidy, and nearly all the embryos die. We have shown that mutations disrupting SCC mediated by REC-8 cohesion, …

Implementing Mindfulness In K-12 Schools: Exploring Educators’ Perceptions Of Students’ Academic Skills, Behavior, And Well-Being, Sara Gottfried

Undergraduate Review

Research indicates that academic performance and social and emotional well-being are fundamentally interrelated (Schonert-Reichl, Oberle, Lawlor, Abbott, Thomson, Oberlander, & Diamond, 2015). Given that 13-20% of children in the United States experience social and emotional challenges, schools are required to attend to the social, emotional, and behavioral needs of students (Maynard, Solis, Miller, & Brendel, 2017). However, students are often unequipped with the skills to effectively cope with stress and resort to behaviors that cause emotional, mental, and physical suffering, all of which thwart the learning process (Schonert-Reichl et al., 2015). Schools warrant interventions that support the whole student, given …

Adapting To College Life: An Ethnographic Study Of The Linguistic Challenges Faced By Immigrant, Black, Male Students At Bridgewater State University, Carter Remy

Undergraduate Review

This linguistic qualitative ethnographic study sought to understand whether immigrant, ESL men who have completed their first year of college at BSU perceive themselves as linguistically prepared for college life. Linguistic preparedness is critical for successful participation in the classroom and completion of required work. The research seeks to identify and analyze the programs BSU has established to assist this population in their adaptation to college life and in acquiring linguistic proficiency. The study employs a multi-tiered methodology, beginning with semi-structured interviews with diversity administrators as well as ESL and Global Language faculty. These interviews were followed by rapport-building, participant …

The Myth Of The Dry Fly Hero: Its Rise And Fall In The Upper Peninsula Of Michigan, John Gubbins

Upper Country: A Journal of the Lake Superior Region

In 1886 Frederic Halford's book Floating Flies and How to Dress Them sparked a global shift in fly fishing from sinking flies, wet flies to floating flies, dry flies. The dry fly purist, an angler who fished only dry flies, became a mythic figure, a moneyed, well educated white man, who banded together with other wealthy white men to form exclusive fishing clubs where only upstream dry fly fishing was allowed. Purists spread the myth of the dry fly hero by publishing books and magazine articles extolling their erudition and expertise as anglers.

For a century the heroic myth of …

Fish Farm: Environmental Outline History Of The Upper Great Lakes Fisheries, Robert Archibald

Upper Country: A Journal of the Lake Superior Region

This article discusses how the fish population in the Upper Peninsula is affected by invasive species, overfishing, and pollution. This includes examining how fishing was first used by Native Americans as a food source, and later became an industrialized business to further negatively impact fisheries by polluting the waters and introducing other species to compete for food sources.

2018 Program, Belmont University

Humanities Symposium Programs

No abstract provided.

Growth Rate And Hatch Date Of Age-0 Fishes On Leech Lake, Minnesota Before Zebra Mussel Establishment, Kayla Morey, Emily Larson

Journal of Earth and Life Science

The introduction of an invasive species to an ecosystem is considered a very severe environmental problem. Zebra Mussel veligers Dreissena polymorpha have recently been found in Leech Lake, Minnesota, and are likely going to have a large influence on this ecosystem. Zebra Mussels are filter-feeders that rely on phytoplankton and zooplankton as their main source of energy. A shift in populations of plankton due to Zebra Mussels may affect the growth rates of larval fish. Median hatch dates and growth rates were estimated from four age-0 fish species (Yellow Perch Perca flavescens, Walleye Sander vitreus, Smallmouth Bass Micropterus …

Fire Blight, Fulya Baysal-Gurel, Chasity A. Phillips

Extension Publications

No abstract provided.

Altruism And Altruistic Love: Intrinsic Motivation For Servant-Leadership, Peter Mulinge

International Journal of Servant-Leadership

In a world where self-interest and selfishness are often a way of life, it is a mystery to see altruistic people commit their lives to the benefit of others, while self-centered people are becoming more isolated and focused on themselves, rather than others. Seltzer (2016) explained that the self-centeredness of narcissists perpetuates their grandiosity, sense of entitlement, lack of empathy, and exploitative relationships. Self-centered narcissists perceive themselves as different from others; they lack understanding of self and value for others at a fundamental level. Understanding of self is very critical in life, it includes knowing one s agendas and the …

The Power Of Play On Academic And Social Learning, Brianna Sorlien

All Electronic Theses and Dissertations

Play is among the most vital components in elementary student’s prosperity, wellbeing and improvement academically and socially. It is a characteristic and instinctual behaviour which should be encouraged during childhood. Some articles indicate how vital playing is for the prosperity and improvement of elementary students, showing why elementary students should have time, support and places to play. They examine the impact of various sorts of play, specifically, outside play and the significance of giving nearby neighbourhood spaces where children feel safe outside with their peers. Different articles recommend fair treatment for elementary students who have special needs. The full acknowledgment …

Extracellular Superoxide Dismutase (Sod3) Regulates Oxidative Stress At The Vitreoretinal Interface, Katherine J. Wert, Gabriel Velez, Madeline R. Cross, Brett A. Wagner, Melissa L. Teoh-Fitzgerald, Garry R. Buettner, J. Jason Mcanany, Alicia Olivier, Stephen H. Tsang, Matthew M. Harper, Frederick E. Domann, Alexander G. Bassuk, Vinit B. Mahajan

United States Department of Veterans Affairs: Publications

Oxidative stress is a pathogenic feature in vitreoretinal disease. However, the ability of the inner retina to manage metabolic waste and oxidative stress is unknown. Proteomic analysis of antioxidants in the human vitreous, the extracellular matrix opposing the inner retina, identified superoxide dismutase-3 (SOD3) that localized to a unique matrix structure in the vitreous base and cortex. To determine the role of SOD3, Sod3^-/- mice underwent histological and clinical phenotyping. Although the eyes were structurally normal, at the vitreoretinal interface Sod3^-/- mice demonstrated higher levels of 3-nitrotyrosine, a key marker of oxidative stress. Pattern electroretinography also showed physiological …

Social Contact Patterns Can Buffer Costs Of Forgetting In The Evolution Of Cooperation, Jeffrey R. Stevens, Jan K. Woike, Lael J. Schooler, Stefan Lindner, Thorsten Pachur

Jeffrey Stevens Publications

Analyses of the evolution of cooperation often rely on two simplifying assumptions: (i) individuals interact equally frequently with all social network members and (ii) they accurately remember each partner's past cooperation or defection. Here, we examine how more realistic, skewed patterns of contact—in which individuals interact primarily with only a subset of their network's members—influence cooperation. In addition, we test whether skewed contact patterns can counteract the decrease in cooperation caused by memory errors (i.e. forgetting). Finally, we compare two types of memory error that vary in whether forgotten interactions are replaced with random actions or with actions from previous …

Mesotocin Influences Pinyon Jay Prosociality, Juan Duque, Whitney Leichner, Holly Ahmann, Jeffrey R. Stevens

Jeffrey Stevens Publications

Many species exhibit prosocial behavior, in which one individual’s actions benefit another individual, often without an immediate benefit to itself. The neuropeptide oxytocin is an important hormonal mechanism influencing prosociality in mammals, but it is unclear whether the avian homologue mesotocin plays a similar functional role in birds. Here, we experimentally tested prosociality in pinyon jays (Gymnorhinus cyanocephalus), a highly social corvid species that spontaneously shares food with others. First, we measured prosocial preferences in a prosocial choice task with two different payoff distributions: Prosocial trials delivered food to both the subject and either an empty cage or a partner …