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2017

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Designing For Urban Coastal Resilience, Phoebe Crisman Jul 2017

Designing For Urban Coastal Resilience, Phoebe Crisman

July 28, 2017: The Next Step to Resilience- Projects and Ideas

No abstract provided.


Coral Vs. Macroalgae: Relative Susceptibility To Sedimentation And Ocean Warming, Ashton J. Galarno Jul 2017

Coral Vs. Macroalgae: Relative Susceptibility To Sedimentation And Ocean Warming, Ashton J. Galarno

HCNSO Student Theses and Dissertations

Sedimentation and ocean warming are two major anthropogenic stressors that directly affect coral recruitment and recovery. Many coral-dominated reefs have undergone phase shifts becoming macroalgae-dominated because of the coral population’s inability to tolerate these increasing stressors. Predicting these phase shifts requires a determination of the relative susceptibility of coral and macroalgae to these stressors. The objective of this study was to quantitatively assess the synergistic effects of sedimentation and elevated temperature on the survival and growth of Montastraea cavernosa newly settled coral juveniles, and fragments of the macroalgae, Dictyota ciliolata. A crossed experimental design tested the two temperatures and …


Evidence Of Intermittent Residency In The Northern Fur Seal (Callorhinus Ursinus)., Megan Foley Jul 2017

Evidence Of Intermittent Residency In The Northern Fur Seal (Callorhinus Ursinus)., Megan Foley

HCNSO Student Theses and Dissertations

This study found evidence of intermittent, multi-year residency periods in northern fur seals (Callorhinus ursinus) using stable isotope ratios in vibrissae and canine teeth. Northern fur seals migrate from the Bering Sea during summer months to lower latitudes and slightly warmer waters of the northeastern Pacific Ocean and California Current in the winter. To determine the length of time spent away from the Bering Sea, growth rate was estimated using the covarying oscillations δ13C and δ15N, estimated to be 0.09 mm/day. The δ13C and δ15N in vibrissae from 30 male …


Investigating The Effect Of Mechanical Beach Cleaning On Nesting, Hatching And Emergence Success Of Loggerhead (Caretta Caretta) And Green (Chelonia Mydas) Sea Turtles In Broward County, Florida, Megan A. Earney Jul 2017

Investigating The Effect Of Mechanical Beach Cleaning On Nesting, Hatching And Emergence Success Of Loggerhead (Caretta Caretta) And Green (Chelonia Mydas) Sea Turtles In Broward County, Florida, Megan A. Earney

HCNSO Student Theses and Dissertations

Sea turtles face many threats to their populations globally. Loggerhead sea turtles (Caretta caretta) and green sea turtles (Chelonia mydas) are listed by the International Union for the Conservation of Nature Red List as Endangered. In Florida, loggerhead and green sea turtles nest along the coastline during April-September. Mechanical beach cleaning is an aesthetic service performed daily on some beaches in Florida to clean the wrack line and/or the entire beach of debris. Alterations made to beaches by methods such as mechanical beach cleaning have the potential to impact sea turtle nesting, hatching, and emergence success. Generalized …


Microbial Community Richness Distinguishes Shark Species Microbiomes In South Florida, Rachael Cassandra Karns Jul 2017

Microbial Community Richness Distinguishes Shark Species Microbiomes In South Florida, Rachael Cassandra Karns

HCNSO Student Theses and Dissertations

The microbiome (microbial community) of individuals is crucial when characterizing and understanding processes that are required for organism function and survival. Microbial organisms, which make up an individual’s microbiome, can be linked to disease or function of the host organism. In humans, individuals differ substantially in their microbiome compositions in various areas of the body. The cause of much of the composition diversity is yet unexplained, however, it is speculated that habitat, diet, and early exposure to microbes could be altering the microbiomes of individuals (Human Microbiome Project Consortium, 2012b, 2012a). To date, only one study has reported on microbiome …


Onciderini Thomson, 1860 (Coleoptera: Cerambycidae: Lamiinae) Types Of The Museum Of Comparative Zoology (Mcz), With A Brief History Of The Coleoptera Collection, Eugenio H. Nearns, Gareth S. Powell Jul 2017

Onciderini Thomson, 1860 (Coleoptera: Cerambycidae: Lamiinae) Types Of The Museum Of Comparative Zoology (Mcz), With A Brief History Of The Coleoptera Collection, Eugenio H. Nearns, Gareth S. Powell

Insecta Mundi

The primary types of Onciderini Thomson, 1860 (Coleoptera: Cerambycidae: Lamiinae) of the Museum of Comparative Zoology (MCZ), Harvard University, are catalogued and illustrated. Data on the original combination, current name, gender, and type locality are verified and presented. There are 19 primary types of Onciderini including seven in Oncideres Lacordaire, 1830; two in Hesychotypa Thomson, 1868; and two in Hypsioma Audinet- Serville, 1835. Of the 19 primary types, 18 were described by L. S. Dillon and E. S. Dillon. A brief history of the Coleoptera collection at the MCZ is also presented.


New Anthophilous Host Associations For Adult Acmaeodera Eschscholtz, 1829 (Coleoptera: Buprestidae) Species From The Western United States And Texas, Richard L. Westcott, Delbert A. La Rue Jul 2017

New Anthophilous Host Associations For Adult Acmaeodera Eschscholtz, 1829 (Coleoptera: Buprestidae) Species From The Western United States And Texas, Richard L. Westcott, Delbert A. La Rue

Insecta Mundi

New anthophilous host associations are presented for adults of 42 species and two subspecies in the buprestid genus Acmaeodera Eschscholtz, 1829 (Coleoptera: Buprestidae), mostly from Arizona and Nevada. Acmaeodera reflexa Barr is reported for the fi rst time from Arizona.


Una Nueva Especie Del Género Eugnosta Hübner, De Chile (Lepidoptera: Tortricidae), Danilo E. Cepeda Jul 2017

Una Nueva Especie Del Género Eugnosta Hübner, De Chile (Lepidoptera: Tortricidae), Danilo E. Cepeda

Insecta Mundi

Una nueva especie del género Eugnosta Hübner, E. lankinae n.sp. (Lepidoptera: Tortricidae), es descrita para la Provincia de Elqui, Chile. Se entregan los caracteres morfológicos del adulto y las fi guras de la genitalia masculina. Además se discute su posición sistemática con dos especies congéneres Neotropicales.

A new species of the genus Eugnosta Hübner, E. lankinae n.sp. (Lepidoptera: Tortricidae), is described from the Elqui Province in Chile. Male adult morphology, including genitalia, are fi gured. The systematic position in relation to two closely related Neotropical species is discussed.


A New Species Of Dipropus Germar (Coleoptera: Elateridae) From Florida, With Taxonomic And Morphological Notes And A New Key To The Species Of The Eastern United States, Blaine Mathison, Paul J. Johnson Jul 2017

A New Species Of Dipropus Germar (Coleoptera: Elateridae) From Florida, With Taxonomic And Morphological Notes And A New Key To The Species Of The Eastern United States, Blaine Mathison, Paul J. Johnson

Insecta Mundi

Dipropus tequesta Johnson new species (Coleoptera: Elateridae) is described from southern Florida. Dipropus fuscus (LeConte) is a new synonym of D. soleatus (Say), and D. granosus (Fall) is a new synonym of D. asper (LeConte). The flightless female of D. asper is described and provides the first report of brachyptery and endogean habits in Dipropus. A key to the species of the eastern United States and a new checklist of species for the country are provided.


Reversion Of Epithelial-Mesenchymal Transition By A Novel Agent Dz-50 Via Igf Binding Protein-3 In Prostate Cancer Cells, Zheng Cao, Shahriar Koochekpour, Stephen E. Strup, Natasha Kyprianou Jul 2017

Reversion Of Epithelial-Mesenchymal Transition By A Novel Agent Dz-50 Via Igf Binding Protein-3 In Prostate Cancer Cells, Zheng Cao, Shahriar Koochekpour, Stephen E. Strup, Natasha Kyprianou

Urology Faculty Publications

Dysregulation of transforming growth factor-β1 (TGF-β1) and insulin-like growth factor (IGF) axis has been linked to reactive stroma dynamics in prostate cancer progression. IGF binding protein-3 (IGFBP3) induction is initiated by stroma remodeling and could represent a potential therapeutic target for prostate cancer. In previous studies a lead quinazoline-based Doxazosin® derivative, DZ-50, impaired prostate tumor growth by targeting proteins involved in focal adhesion, anoikis resistance and epithelial-mesenchymal-transition (EMT). This study demonstrates that DZ-50 increased expression of the epithelial marker E-cadherin, and decreased the mesenchymal marker N-cadherin in human prostate cancer cells. In DU-145 cells, the effect of DZ-50 on EMT …


Induction Of Mir-155 After Brain Injury Promotes Type 1 Interferon And Has A Neuroprotective Effect., Emily Harrison, Kathleen M. Emanuel, Benjamin G. Lamberty, Brenda M. Morsey, Min Li, Matthew L. Kelso, Sowmya V. Yelamanchili, Howard S. Fox Jul 2017

Induction Of Mir-155 After Brain Injury Promotes Type 1 Interferon And Has A Neuroprotective Effect., Emily Harrison, Kathleen M. Emanuel, Benjamin G. Lamberty, Brenda M. Morsey, Min Li, Matthew L. Kelso, Sowmya V. Yelamanchili, Howard S. Fox

Journal Articles: Pharmacology & Experimental Neuroscience

Traumatic brain injury (TBI) produces profound and lasting neuroinflammation that has both beneficial and detrimental effects. Recent evidence has implicated microRNAs (miRNAs) in the regulation of inflammation both in the periphery and the CNS. We examined the expression of inflammation associated miRNAs in the context of TBI using a mouse controlled cortical impact (CCI) model and found increased levels of miR-21, miR-223 and miR-155 in the hippocampus after CCI. The expression of miR-155 was elevated 9-fold after CCI, an increase confirmed by in situ hybridization (ISH). Interestingly, expression of miR-155 was largely found in neuronal nuclei as evidenced by co-localization …


Thermal Behavior And Decomposition Kinetics Of Nerol-Β-D-Glycoside, Lei Sheng, Zhao Yingliang, Yang Qianxu, Xia Jianjun, Ding Meiyu, Yang Xihong, Xie Wancui, Zhang Tiandong Jul 2017

Thermal Behavior And Decomposition Kinetics Of Nerol-Β-D-Glycoside, Lei Sheng, Zhao Yingliang, Yang Qianxu, Xia Jianjun, Ding Meiyu, Yang Xihong, Xie Wancui, Zhang Tiandong

Food and Machinery

Thermal decomposition was studied by DTA/SDTG to indicate the thermal degradation mechanism of a natural identifical flavor precursor of nerol-β-D-glucopyranoside. The heating rate was adapted of 5, 10, and 15 ℃/min in the temperature range from 30 to 500 ℃. The parameters were obtained by Friedman method and Ozawa-Flynn-Wall method. The TG-DTG curves showed that with the increase of heating rate, the thermal degradation temperature was mainly determined by the heating rate B. Initial temperature of mass reduction was T0=1.102 3B+199.4, and that at the maximum rate was Tp=2.102 3B+251.9. However, the final temperature of mass …


Thermal Process Pattern And Numerical Simulation Of Fen Liquor Fermentation In Underground Vats, Han Ying, Chen Jianxin Jul 2017

Thermal Process Pattern And Numerical Simulation Of Fen Liquor Fermentation In Underground Vats, Han Ying, Chen Jianxin

Food and Machinery

In order to understand the characteristics and mechanism of the thermal process of liquor fermentation system, which have great significance to the temperature control of the fermentation process and the mechanization of the fermentation system. The temperature distribution of fermented grains and the soil surrounding the cylinder are compared in different seasons. The process of biological heat generation and diffusion during the Fen liquor fermentation was confirmed. The temperature variation characteristics of fermented grains and heat conduction is the main mode of heat transfer in fermentation is determined. The important influence of temperature on the thermal process of fermentation is …


Contrastive Analysis Of Volatile Compounds From Fresh Adlay Seeds And Those With Off-Flavor, Yang Zhongquan, Kan Jianquan, Chen Guangjing, Xia Ji Jul 2017

Contrastive Analysis Of Volatile Compounds From Fresh Adlay Seeds And Those With Off-Flavor, Yang Zhongquan, Kan Jianquan, Chen Guangjing, Xia Ji

Food and Machinery

Headspace-solid phase micro-extraction (HS-SPME) coupled to gas chromatography-mass spectrometry (GC-MS) was applied to analyze the volatile compounds of the fresh adlay seeds and those with off-flavor. The results showed: more than 85 of volatile compounds which belonged to distinct chemical families were analyzed. A total of 47 compounds in fresh adlay seeds were identified (including 12 alcohols, 11 aldehydes and ketones and 22 hydrocarbons), the major volatile components in fresh adlay seeds were (+)-Limonene, benzene, 2-pentyl furan, hexanal, 1-nonanal, n-octanal, 2-heptanone, 1-pentanol, 1-hexanol, 1-octen-3-ol and linalool; While, there were 58 compounds in adlay seeds with off-flavor (including 17 hydrocarbons, 8 …


Optimization Of Enzymatic Hydrolysis Process And Analysis Of Structural Properties On Bovine Bone Crude Collagen, Liu Lili, Li Dan, Yang Chenliu, Meng Yuanyuan, Dai Xiaoning Jul 2017

Optimization Of Enzymatic Hydrolysis Process And Analysis Of Structural Properties On Bovine Bone Crude Collagen, Liu Lili, Li Dan, Yang Chenliu, Meng Yuanyuan, Dai Xiaoning

Food and Machinery

In this work, the enzymatic hydrolysis of bovine bone collagen was studied by pre-purified bone-specific collagenase (BSC). As evaluated in terms of degree of hydrolysis. The enzymatic hydrolysis process was studied through single factor and quadratic general rotary unitized design experiments. The results showed that the optimum hydrolysis conditions were as follows: reaction temperature 46 ℃, the amount of bovine bone collagen 5.14 g/100 mL, the amount of BSC enzyme 0.42 g/100 mL, reaction time 6 h, pH 6.5. Under these conditions, the degree of hydrolysis value was 34.98%. The structural properties of bovine bone collagen and its hydrolysate were …


Study On Inhibition Kinetics Of Peroxidase From Lonicera Japonica Thunb, Luo Lei, Dong Jinlong, Zhu Wenxue, Xue Yihan Jul 2017

Study On Inhibition Kinetics Of Peroxidase From Lonicera Japonica Thunb, Luo Lei, Dong Jinlong, Zhu Wenxue, Xue Yihan

Food and Machinery

In order to purify honeysuckle peroxides and study the enzymatic properties and inhibition kinetics,DEAE cellulose DE-52 ion exchange chromatography was used for purification of peroxidase from Lonicera Japonica Thunb prepared preliminarily by three-phase partitioning. Two kinds of peroxidase, HSPⅠ(honeysuckle peroxidaseⅠ)and HSPⅡ(honeysuckle peroxidaseⅡ), were purified,the specific activity were 3 312.3 U/mg and 564.8 U/mg respectively. The double substrate enzymatic reaction type was PingPong reaction analyzed by the reaction kinetics. Citric acid, Sodium metabisulfite showed irreversible inhibition to Lonicera Japonica Thunb peroxidase, L-cysteine showed reversible inhibition, and L-cysteine was a competitive inhibitor that the inhibition constant KI was 0.053 mmol/L.


Effect Of Uv-C Treatment On Quality Of Blueberries Fruit In Low-Temperature Storage, Zhang Juhua, Li Gaoyang, Wang Wei, Lin Shuhua, Liu Wei, Tan Huan Jul 2017

Effect Of Uv-C Treatment On Quality Of Blueberries Fruit In Low-Temperature Storage, Zhang Juhua, Li Gaoyang, Wang Wei, Lin Shuhua, Liu Wei, Tan Huan

Food and Machinery

In order to develop green preservation technology of blueberry, Southern highbush blueberries were treated by using different doses of UV-C (1.0, 2.0, 4.0 kJ/m2), and storaged at (4±0.5) ℃, changes of the quality were measured every 7 d during storage period, control samples was untreated. The results showed that UV-C treatment had better effect on improving the storage quality of blueberry, and 2 kJ/m2 treatment was the best on preservation effect of blueberry, which can effectively inhibit weight loss and rotting of postharvest blueberry; when storaged for 35 days and compared to the control group, the weight …


Effects Of Hypobaric And Refrigerated Preservation On Postharvest Quality Of Mango Fruit, Xie Jianhua, Wen Jianling, Zheng Junfeng, Guo Qiaoling, Pang Jie Jul 2017

Effects Of Hypobaric And Refrigerated Preservation On Postharvest Quality Of Mango Fruit, Xie Jianhua, Wen Jianling, Zheng Junfeng, Guo Qiaoling, Pang Jie

Food and Machinery

The effect of different hypobaric conditions on the storage quality of postharvest mangos with Tainong No. 1 is studied in this paper. The results showed that the hypobaric and refrigerated preservation technology could effectively slow down the reduction of titratable acid, VC and soluble solids content of the fruit, keep the hardness of mango, inhibit the yellowing of mango and the increasing of membrane permeability. The preservation effect of 3 kinds of pressure by comparison showed that the storage quality of mango under the 10 kPa and the 20 kPa was better than under the 30 kPa, the mango …


Prediction Model For The Shelf-Life Of Ponkan Fruit Wine Based On Sensory And Physic-Chemical Indexes, Li Chong, Ma Chengjin, Yu Ji, Zou Haiying, Peng Zhenzhen Jul 2017

Prediction Model For The Shelf-Life Of Ponkan Fruit Wine Based On Sensory And Physic-Chemical Indexes, Li Chong, Ma Chengjin, Yu Ji, Zou Haiying, Peng Zhenzhen

Food and Machinery

According to the analysis of sensory, physic-chemical characteristics(VFA) and microbiological characteristics (total bacterial) for Ponkan fruit wine, using Weibull Hazard Analysis(WHA),chemical reaction kinetic model and Arrhenius equation, the shelf-life prediction models were established under two different kinds of indexes, respectively. The results showed that the shelf-life end point under different storage temperature of 25,30,35 and 40 ℃ by the prediction model of sensory evaluation were 620, 436, 310 and 222 d, respectively, and the relative error was in the range of -5.48% to 5.52%. The predicted end points of the shelf-life under the evaluation index of volatile acid were 633, …


Study On Correlation Between Contents Of Active Substances And Antioxidant Activity In Different Varieties Of Aronia Melanocarpa, Liao Xia, Li Weizhou, Zheng Shaojie, Shi Fang, Ming Jian Jul 2017

Study On Correlation Between Contents Of Active Substances And Antioxidant Activity In Different Varieties Of Aronia Melanocarpa, Liao Xia, Li Weizhou, Zheng Shaojie, Shi Fang, Ming Jian

Food and Machinery

This study aimed to investigate the correlation between the content of active substances and antioxidant activity in three kinds of Aronia Melanocarpa. The total phenolic content was determined by Folin-Ciocalteu method, the anthocyanins content was determined by pH-different method, and the flavonoids was determined by Al(NO3)3-NaNO2 system method. Then, determining the DPPH+· scavenging capacity, ABTS+· scavenging capacity and reducing power of active substances. It was shown that the phenolic content ranged from 11.916 to 14.550 mg GAE/g·FW, which have the best antioxidant properties, the DPPH+· scavenging capacity and ABTS …


Effects Of Different Heat Treatment Onqualities Of Indicarice Grains And Semidry-Milled Rice Flours, Ren Mengying, Zhou Sumei, Tong Litao, Yi Cuiping Jul 2017

Effects Of Different Heat Treatment Onqualities Of Indicarice Grains And Semidry-Milled Rice Flours, Ren Mengying, Zhou Sumei, Tong Litao, Yi Cuiping

Food and Machinery

To investigate the influences of different heat treatment on attributes of Indica rice grains and semidry-milled rice flours, rice grain was treated with differen the attreatment.The relationship between the apparent cracks of rice grain and its moisture content were analyzed. And then made the semidry-milled rice flour after heat treatment of rice grains, the changes of microbial decomination, damaged starch content and whiteness in rice flours were determined.The results showed that the water content when the surface fissures of rice grains became increased was less than 8%. The semidry-milled rice flours after three heat treatment methods(60 ℃ 90 min, 120 …


Research Progress Of Peony (Paeonia Suffruticosa Andr.) Flavonoids, Wang Hongxin, Wu Ying, Kou Xingran, Ma Chaoyang, Guo Xun, Huang Tie Jul 2017

Research Progress Of Peony (Paeonia Suffruticosa Andr.) Flavonoids, Wang Hongxin, Wu Ying, Kou Xingran, Ma Chaoyang, Guo Xun, Huang Tie

Food and Machinery

This review focused on the recent progress of peony (Paeonia suffruticosa Andr.) flavonoids. Extraction, separation, purification methods of peony flavonoids as well as their biological activities were summarized. An outlook on the peony flavonoids was also put forward for future research.


Advances On Bioactive Compounds Of By Products From Hyriopsis Cumingii, Dong Ye, Yu Ting, Wang Jianhui, Wang Yunxiang, Fan Li, Wang He Jul 2017

Advances On Bioactive Compounds Of By Products From Hyriopsis Cumingii, Dong Ye, Yu Ting, Wang Jianhui, Wang Yunxiang, Fan Li, Wang He

Food and Machinery

Triangle pearl mussel (Hyriopsis cumingii) is a superior Chinese freshwater pearl mussel species. After harvesting the pearl from the mussel large quantities of by-products are generated as waste, which includes meat, mantle and mussel tears. However, it is known that processing by-products are rich sources of nutritional substances. The present study briefly analyzed the chemical composition and characterization of mussel by-products. Especially, this paper summarized the research progress of bioactive compounds from mussel by-products such as polysaccharide, protein and peptide. Future research prospects of bioactive compounds from mussel by-products are also outlined.


Study On The Nano-Starch And Its Application In Food Industry, Chen Qijie, Zheng Xueming, Zhou Liling, Dong Xufang, Wang Jianhui Jul 2017

Study On The Nano-Starch And Its Application In Food Industry, Chen Qijie, Zheng Xueming, Zhou Liling, Dong Xufang, Wang Jianhui

Food and Machinery

Nano-starch has many excellent properties such as rich source, non-toxic, renewable, biodegradable and good biocompatibility, but also has the nano characteristics showing effects of the small and quantum size, surface and interface, and macroscopic quantum tunneling, which was widely used in food industry. The main aim of this review was to introduce preparation and modification methods, and physicochemical properties of nano-starch. Meantime, the application of nano-starch in food industry was also reviewed and prospected.


Plant Anthocyanin Exert Antitumor Effect And Mechanism Of By Jnk Signaling Pathway, Zhi Qi, Li Fuxiang, Yang Yaxuan, Tian Yong, Tang Yu, Shi Fang, Ming Jian Jul 2017

Plant Anthocyanin Exert Antitumor Effect And Mechanism Of By Jnk Signaling Pathway, Zhi Qi, Li Fuxiang, Yang Yaxuan, Tian Yong, Tang Yu, Shi Fang, Ming Jian

Food and Machinery

JNK signaling pathway plays an important role in the pathogenesis and development of tumor. In this study, the composition and activation mechanism of JNK signaling pathway was reviewed. Moreover, the ability of inhibiting the proliferation, invasion and apoptosis of breast cancer, prostate cancer, liver cancer, etc by plant anthocyanin was summarized, in order to provide a certain basis for the application of plant anthocyanins in developing clinical medicine and drugs.


The Impact Of Climate And Elevation On The Growth And Mortality Of Piñon Pine, Alice M. Fretz Jul 2017

The Impact Of Climate And Elevation On The Growth And Mortality Of Piñon Pine, Alice M. Fretz

Biology ETDs

The Southwestern United States is currently experiencing severe drought, resulting in the mortality of many tree species. Piñon-juniper woodlands are an extensive biome in the Southwest, and are highly vulnerable to extended periods of drought that lead to tree mortality. Specifically, Pinus edulis populations are decreasing due to increasingly arid conditions. I used dendrochronology to investigate how tree growth rings of Pinus edulis reflect severe drought in living and dead trees. I also investigated how severe drought affects Pinus edulis along an altitudinal gradient. Tree core samples were taken from currently living and dead trees, as well as from trees …


A Dna Origami Platform For Quantifying Protein Copy Number In Super-Resolution, Francesca Cella Zanacchi, Carlo Manzo, Angel S. Alvarez, Nathan D. Derr, Maria F. Garcia-Parajo, Melike Lakadamyali Jul 2017

A Dna Origami Platform For Quantifying Protein Copy Number In Super-Resolution, Francesca Cella Zanacchi, Carlo Manzo, Angel S. Alvarez, Nathan D. Derr, Maria F. Garcia-Parajo, Melike Lakadamyali

Biological Sciences: Faculty Publications

Single-molecule-based super-resolution microscopy offers researchers a unique opportunity to quantify protein copy number with nanoscale resolution. However, while fluorescent proteins have been characterized for quantitative imaging using calibration standards, similar calibration tools for immunofluorescence with small organic fluorophores are lacking. Here we show that DNA origami, in combination with GFP antibodies, is a versatile platform for calibrating fluorophore and antibody labeling efficiency to quantify protein copy number in cellular contexts using super-resolution microscopy.


Molecular And Culture-Based Assessment Of Root/Crown Rot Fungal/Oomycete Complex Of Bean In Mozambique, Suzana Vanessa Ismael Fernandes Jul 2017

Molecular And Culture-Based Assessment Of Root/Crown Rot Fungal/Oomycete Complex Of Bean In Mozambique, Suzana Vanessa Ismael Fernandes

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Dry edible beans (Phaseolus vulgaris) play an important role in food security, alleviating hunger and malnutrition and adding value to the economy in Mozambique. Recently, root/crown rot (RCR) has caused yield losses in bean fields in Mozambique. A diverse taxa of fungi and oomycetes have been associated with RCR. However, in Mozambique little information on root rot is available. The purpose of this study is to use Illumina sequencing platform, polymerase chain reaction (PCR) and culture-based methods to identify the primary pathogens associated with RCR in Mozambique. A total of 88 plants showing symptoms of RCR disease were …


Improving Health And Wellness For Persons With Chronic And Disabling Conditions: Evidence For Practice, Alexa Stuifbergen Jul 2017

Improving Health And Wellness For Persons With Chronic And Disabling Conditions: Evidence For Practice, Alexa Stuifbergen

International Nursing Research Congress (INRC)

This presentation will summarize a 25-year program of research describing the impact of health promotion behaviors on quality of life for persons with a range of chronic and disabling conditions. Findings from studies using descriptive and experimental designs and qualitative and quantitative methods will be discussed.


Impacting Congenital Abnormalities And Infectious Complications During Pre-Conception Through Pregnancy: The Zika Prevention Program (Zpp), Pamela Allyn Di Vito-Thomas Jul 2017

Impacting Congenital Abnormalities And Infectious Complications During Pre-Conception Through Pregnancy: The Zika Prevention Program (Zpp), Pamela Allyn Di Vito-Thomas

International Nursing Research Congress (INRC)

The purpose of this evidence-based practice project is to investigate the Zika Prevention Program (ZPP) compared to current guidelines in decreasing the rate of congenital abnormalities and infectious complications during pre-conception through pregnancy.