Digital Commons Network^™

Negotiating Desire: Resisting, Reimagining And Reinscribing Normalized Sexuality And Gender In Fan Fiction, Charity A. Fowler

Theses and Dissertations

Fan studies has examined how fan fiction resists heteronormativity by challenging depictions of gender and sexuality, but to date, this inquiry has focused disproportionately on slash, to the exclusion of other genres of fan fiction. Additionally, scholars disagree about slash’s subversive effects by setting up a seemingly stable dichotomy—subversive vs. misogynistic—where one does not necessarily exist.

In this project, I examine multiple genres of fan fiction—namely, slash arising from bromances; femslash from female friendships; incestuous fan fiction from dysfunctional familial relationships; and polyamorous fics. I chose fics from four televisions shows—NBC’s Revolution, MTV’s Teen Wolf, the CW’s The …

Tissue Factor Expression, Regulation, And Signaling In Human Airway Cells, Michael D. Davis

Theses and Dissertations

Rationale: Tissue Factor (TF) is a transmembrane glycoprotein that canonically functions as the initiator of the coagulation cascade. Increased levels of TF have been associated with inflammatory airway diseases. Since lipopolysaccharide (LPS) is known to elicit and inflammatory response in airway epithelium, we hypothesized that airway epithelial cells release TF when exposed to LPS. Since TF aids in local wound healing, we also hypothesized that inhibition of TF would decrease NHBE growth. The specific aim of this work was to evaluate the effects of LPS exposure on TF production and release from airway epithelia and determine the signaling pathways involved. …

Short-Term Effects Of Nutrients On A Barrier Island Grassland Community, Ashley Moulton

Theses and Dissertations

Increased nutrient availability globally has the potential to affect community functional composition of plants in nutrient limited environments, such as coastal grassland systems. Stability of these systems are threatened worldwide by urbanization, as well as effects of sea level rise and increased frequency and intensity of storms, and atmospheric N deposition, associated with climate change. Annual net primary productivity (ANPP), species composition, and functional traits (community weighted specific leaf area (CWSLA), leaf area index (LAI), growth form and photosynthetic pathway) were measured across four treatments to assess multiple resource limitation of nitrogen (N) and phosphorus (P) and functional community response …

Sleep Disturbances And Depression: The Role Of Genes And Trauma, Mackenzie J. Lind

Theses and Dissertations

Sleep disturbances and insomnia are prevalent, with around 33% of adults indicating that they experience at least one main symptom of insomnia, and bidirectional relationships exist with common psychopathology, particularly major depressive disorder (MDD). However, genetic and environmental (e.g., traumatic event exposure) contributions to the etiology of these phenotypes are not yet well understood. A genetically informative sample of approximately 12,000 Han Chinese women aged 30-60 (50% with recurrent MDD) was used to address several gaps within the sleep literature. Sleep disturbances were assessed in all individuals using a general item addressing sleeplessness (GS). A sleep within depression sum score …

Protective Factors In The Association Between Child Sexual Abuse And Telomere Length In Adults, David Sosnowski

Theses and Dissertations

The purpose of the present study was to examine if childhood sexual abuse (CSA) was associated with decreases in mean telomere length (TL), and if social support and/or optimism moderated this association. The study included 99 Caucasian female monozygotic twins, ranging in age from 19-48 (M_age = 30.5, SD = 7.8) at Time 1. Linear mixed effects models were employed to test study hypotheses. Analyses with all participants did not detect an effect of CSA exposure or severity on mean TL, nor were there effects with optimism. However, in analyses that only included women exposed to abuse, increases …

Sol-Gel Chemistry: An Advanced Technique To Produce Macroscopic Nanostructures Of Metal And Semiconductor Colloids, Lamia Nahar

Theses and Dissertations

The fascinating physical properties that arise in materials limited to dimensions of 1-100 nm have gained noteworthy interest from the scientific community. Accordingly, there has been a lot of attention paid to the synthesis of discrete nanoparticles (NPs) and they are being investigated for a range of advanced technologies. Nonetheless, efficient use of nanomaterials in device applications require them to be assembled into solid state macro-structures while retaining their unique, nanoparticulate properties. To date, most commonly investigated assembling techniques include: covalent coupling of NPs surface groups, control evaporation of the solvent to produce ordered supercrystals or non-ordered glassy films, and …

Po2 Dependence Of Oxygen Consumption In Skeletal Muscle Of Diabetic And Non-Diabetic Rats, Alexander C. Liles

Theses and Dissertations

Abstract

PO₂ DEPENDENCE OF OXYGEN CONSUMPTION IN SKELETAL MUSCLE OF DIABETIC AND NON-DIABETIC RATS

By: Alexander C. Liles

A thesis submitted in partial fulfillment of the requirements for the degree of Master of Science at the Medical College of Virginia Campus, Virginia Commonwealth University

Virginia Commonwealth University, 2017

Advisor: Roland N. Pittman, Ph.D.

Department of Physiology and Biophysics

Type 2 diabetes mellitus (T2DM) is a major medical problem around the world, affecting nearly 6% of the world’s population. This study was an attempt to better understand physiological changes the disease may cause to the microcirculation and more specifically, to …

A Novel Synthesis And Subsequent Decyclization Of Iminothiozolidinones: Expansion Of Thiourea Chemistry For Biological Applications, Constance D. Franklin

Theses and Dissertations

Small molecule synthesis has become a valuable tool in the study of biological systems. Biologically active compounds can be designed based on well-characterized endogenous systems or they can be found through the screening of large libraries of small molecules. This work involves the development of a small library of cyclic thiourea-based small molecules by use of an unreported synthetic pathway. Briefly, parent thioureas were cyclized by reaction with bromoacetyl bromide, and one or two isomeric heterocycles were found to form. Further studies indicated that the reaction could be easily manipulated by temperature or solvent to effectively control the product distribution. …

Synchrony And Concordance: A Multilevel Analysis Of The Effects Of Individual Differences During A Co2 Challenge, Rachel E. Wallace

Theses and Dissertations

Emotion theories posit that emotion systems (e.g., behavior, self-report, physiology) should be related when an emotion is being elicited because this serves an adaptive purpose and allows the individual to respond appropriately to the present situation. Oftentimes, this coherent relationship is not found, and research has hypothesized that the type of analyses used and lack of examination of individual differences could be affecting this relationship. Most studies examine the relationship between emotion systems between-subjects when within-subjects analyses may be more appropriate. The present study examined the relationship between self-reported distress (SUDS) and heart rate, and whether trait differences of anxiety …

A Range-Wide Assessment Of Migratory Connectivity For The Prothonotary Warbler Using Stable Hydrogen Isotopes, Jessica Reese

Theses and Dissertations

Populations of many species of migratory birds are declining, and an understanding of how populations are linked between the breeding and nonbreeding grounds is necessary in order to determine drivers of declines. While all current tracking technologies to study the movements of small songbirds are limited by their coarse resolution, tracking birds by measuring stable isotopes in keratin offers an advantage because it requires only a single capture and is cost-effective, which leads to robust sample sizes. While this tracking method is accurate, stable hydrogen isotope values measured in feathers (δ²H_f) are known to be variable …

The Institute Of New Feelings: Plastic Identities And Imperfect Surfaces, Weijian Zhou

Theses and Dissertations

Digital media are moldable spaces where an image is simultaneously a thought. This instance and flexibility enables digital existences to be malleable, transformative, situational, and unstable. They are plastic images. Video games generate digital bodies that are a fusion of subjectivities and cybernetic simulations, in a perceivable and ambiguous process. Such bodies are extensions of ourselves, being girlish, imperfect, unfinished and happening—digesting and emitting clusters of feelings, regardless of our biological gender and age. The performative experience of play is progressively departing from spectacle, gambling and competition, and increasingly shifting towards an emotional journey of alternate realities, spreading subjectivities into …

Comparison Of Intraoral And Extraoral Scanners On The Accuracy Of Digital Model Articulation, Jason L. Porter

Theses and Dissertations

Introduction: Orthodontists increasingly rely on digital models in clinical practice. The ability of modern scanners to articulate digital models must be scientifically evaluated. Methods:Twenty five digital articulated models were produced from four digital scanners in five experimental groups. The resulting inter-arch measurements were compared to the gold standard. An acceptable range of 0.5mm more or less than the gold standard was used for evaluation. Results: iTero® and iTero® Element yielded all acceptable inter-arch measurements. The 3M™ True Definition and Ortho Insight 3D® with Regisil® bite registration produced four of six acceptable inter-arch measurements. The Ortho Insight 3D® with Coprwax ™ …

A Multi-Method Exploration Of The Genetic And Environmental Risks Contributing To Tobacco Use Behaviors In Young Adulthood, Elizabeth K. Do

Theses and Dissertations

Tobacco use remains the leading preventable cause of morbidity and mortality in both the United States and worldwide. Twin and family studies have demonstrated that both genetic and environmental factors are important contributors to tobacco use behaviors. Understanding how genes, the environment, and their interactions is critical to the development of public health interventions that focus on the reduction of tobacco related morbidity and mortality. However, few studies have examined the transition from adolescent to young adulthood – the time when many individuals are experimenting with and developing patterns of tobacco use. This dissertation thesis seeks to provide a comprehensive …

Chemical Probes For Protein Α-N-Terminal Methylation, Brianna D. Mackie

Theses and Dissertations

While protein α-N-terminal methylation has been known for nearly four decades since it was first uncovered on bacteria ribosomal proteins L33, the function of this modification is still not entirely understood. Recent discoveries have demonstrated α-N-terminal methylation is essential to stabilize the interactions between regulator of chromosome condensation 1 (RCC1) and chromatin during mitosis, to localize and enhance the interaction of centromere proteins (CENPs) with chromatin, and to facilitate the recruitment of DNA damage-binding protein 2 (DDB2) to DNA damage foci. Identification of N-terminal methyltransferase 1 (NTMT1) unveiled the eukaryotic methylation writer for protein α-N-termini. In addition, NTMT2 that shares …

Development Of My Footprint Calculator, Karthik Mummidisetti

Theses and Dissertations

The Environmental footprint is a very powerful tool that helps an individual to understand how their everyday activities are impacting environmental surroundings. Data shows that global climate change, which is a growing concern for nations all over the world, is already affecting humankind, plants and animals through raising ocean levels, droughts & desertification and changing weather patterns. In addition to a wide range of policy measures implemented by national and state governments, it is necessary for individuals to understand the impact that their lifestyle may have on their personal environmental footprint, and thus over the global climate change. “My Footprint …

In Search Of A Function For An Uncharacterized Conserved Protein In Streptococcus Sanguinis Sk36, Ayana Scott-Elliston

Theses and Dissertations

With the number of fully sequenced bacterial genomes increasing in the past 7 years, it has been discovered that a large percentage of the putative protein coding genes have no known function. This lack of knowledge leaves scientists with an incomplete understanding of bacteria. In this study, conserved hypothetical protein mutants from Streptococcus sanguinis SK36 were screened on solid media with various environmental conditions. From these screens, the candidate protein, SSA_2372, displayed a sensitivity to acidic conditions. Its homolog in Bacillus subtilis 168, BSU00030, also displayed a sensitivity to pH conditions at its acid tolerance extremes unlike its other homolog …

Effects Of Prescribed Fire On Cope’S Gray Treefrog (Hyla Chrysoscelis) Across Habitat Scales And Life Stages, Logan Mcdonald

Theses and Dissertations

Fire may alter both aquatic and terrestrial habitat used by all amphibian life stages, yet, our knowledge of its effects on amphibians is primarily limited to adult responses. I present an integrated approach to test the response of Cope’s Gray Treefrog (Hyla chrysoscelis) to fire by examining responses in tadpole performance and survivorship, adult abundance, and oviposition. Tadpoles raised with burned leaf litter had similar survival, but total mass and total length were 440% and 170% greater, respectively, for tadpoles raised in unburned litter. I assessed terrestrial and aquatic oviposition cues by embedding burned and unburned litter treatments within burned …

Comparisons Of Isogenic Trisomic And Disomic Cells From People With Mosaicism For Down Syndrome Unmask Cellular Differences Related To Trisomy 21, Kelly A. Rafferty

Theses and Dissertations

It is known that age-related changes impacting multiple organ systems occur earlier in people with Down syndrome (Ds), but the biological basis underlying this trisomy 21-associated propensity for premature aging is poorly understood. Given that the trisomic/normal cells from people with mosaic Ds (mDs) are identical with regards to environmental exposures and genes (except for chromosome 21 copy number), comparisons of these isogenic trisomic/disomic cells allow one to “unmask” the cellular consequences of trisomy 21 by removing extraneous factors. The primary aim of this study was to determine if trisomy 21 results in an increase in the acquisition of age-related …

D Is For The Most Cherished Sense (Whence It Comes And Wither It Goes), Hallie S. Mcneill

Theses and Dissertations

A transcript of the audio that constitutes the work by the same title, along with an introduction and relevant bibliography.

Regulation Of Hnrnpa1 By Micrornas Controls The Mir-18a–K-Ras Axis In Chemotherapy-Resistant Ovarian Cancer, Cristian Rodriguez-Aguayo, Paloma Del C Monroig, Roxana S Redis, Emine Bayraktar, Maria I Almeida, Cristina Ivan, Enrique Fuentes-Mattei, Mohammed H Rashed, Arturo Chavez-Reyes, Bulent Ozpolat, Rahul Mitra, Anil K Sood, George A Calin, Gabriel Lopez-Berestein

Faculty, Staff and Student Publications

The regulation of microRNA (miRNA) biogenesis, function and degradation involves a range of mechanisms, including interactions with RNA-binding proteins. The potential contribution of regulatory miRNAs to the expression of these RNA interactor proteins that could control other miRNAs expression is still unclear. Here we demonstrate a regulatory circuit involving oncogenic and tumor-suppressor miRNAs and an RNA-binding protein in a chemotherapy-resistant ovarian cancer model. We identified and characterized miR-15a-5p and miR-25-3p as negative regulators of hnRNPA1 expression, which is required for the processing of miR-18a-3p, an inhibitor of the

An Exploration Of Anchoring Female Millennial Students To An It Career Path: The Class Model, Cherie Noteboom, Pamela Rowland

Research & Publications

There has been much research conducted on the under-representation of women in Science, Technology, and Engineering & Math (STEM). Looking specifically at the area of Information Systems and Information Technology (IS/IT), women are largely under-represented in degree programs and the workforce. With the growth in the IS/IT workforce and the future demands of IS/IT career growth, the importance of understanding the perceptions and influences on the female IS/IT students grows. While there is research investigating the demographics of the IS/IT workforce, the relationship between female students and millennial influences has not been investigated. This research contends that addressing the demographic …

Mursamacin: A Novel Class Of Antibiotics From Soil-Dwelling Roundworms Of Central Kenya That Inhibits Methicillin-Resistant Staphylococcus Aureus, Ryan Musumba Awori, Peter Njenga Ng'ang'a, Lorine Nanjala Nyongesa, Nelson Onzere Amugune, Daniel Masiga

All Peer-Reviewed Publications

Antibiotic-resistant bacteria, also called " superbugs", can at worst retrogress modern medicine to an era where even sore throats resulted in death. A solution is the development of novel types of antibiotics from untapped natural sources. Yet, no new class of antibiotic has been developed in clinical medicine in the last 30 years. Here, bacteria from insect-killing Steinernema roundworms found in the soils of Central Kenya were isolated and subjected to specific molecular identification. These were then assayed for production of antibiotic compounds with potential to treat methicillin-resistant Staphylococcus aureus infections. The bacteria were identified as Xenorhabdus griffiniae and produced …

Cotesia Icipe Sp. N., A New Microgastrinae Wasp (Hymenoptera, Braconidae) Of Importance In The Biological Control Of Lepidopteran Pests In Africa, Komi K.M. Fiaboe, Jose Fernández-Triana, Faith W. Nyamu, Komi M. Agbodzavu

All Peer-Reviewed Publications

A new species of Microgastrinae, Cotesia icipe Fernández-Triana & Fiaboe, sp. n., is described from eastern Africa. It was reared in Kenya as a solitary parasitoid from two major amaranth pests, Spodoptera littoralis (Boisduval, 1833) and S. exigua (Hübner, 1808); study of specimens in collections also revealed its presence in four other countries in the Afrotropical region (Madagascar, Saudi Arabia, South Africa, and Yemen). Morphological, molecular and biological characters are used to describe the new species and to distinguish it from all 12 previously described species of Afrotropical Cotesia. Cotesia icipe shows potential in the biological control of key Lepidopteran …

Impact Of Push-Pull Technology On The Nutritional Status Of Farmers' Children In Western Kenya, Nicholas O. Ogot, J. O. Pittchar, C. A.O. Midega, Z. R. Khan

All Peer-Reviewed Publications

This study examined the impact of push-pull technology (PPT) on the nutritional status of children aged 1-12 years. Non-push-pull (NPPT) farmers were used as a control group to establish a comparative model for this study. It determined household production, consumption, and surpluses, comparing the PPT adopters to the nonadopters; found out the incomes and food expenditures from farm products; found out the household dietary diversity scores; and finally found the nutritional status of the two household groups. A six faceted household-level metrics was employed. A sample of 216 households that registered 326 children was derived. This study was conducted in …

Magnetic Field Sensing Using Whispering Gallery Modes In A Cylindrical Microresonator Infilitrated With Ferronematic Liquid Crystal, Aseel Mahmood, Vishnu Kavungal, Sudad S. Ahmed, Peter Kopcansky, Vlasta Zavisova, Gerald Farrell, Yuliya Semenova

Articles

An all-fiber magnetic field sensor based on whispering-gallery modes (WGM) in a fiber micro-resonator infiltrated with ferronematic liquid crystal is proposed and experimentally demonstrated. The cylindrical microresonator is formed by a 1 cm-long section of a photonic crystal fiber infiltrated with ferronematic materials. Both ferronematics suspensions are prepared based on the nematic liquid crystal 1-(trans-4-Hexylcyclohexyl)-4-isothiocyanatobenzene (6CHBT) doped with rod-like magnetic particles in the first case and with spherical magnetic particles in the second case. WGMs are excited in the fiber microresonator by evanescent light coupling using a tapered fiber with a micron-size diameter. The Q-factor of the microresonator determined from …

Strength Property Variability In Microbial Induced Calcite Precipitation Soils, Jacob Fuller

UNF Graduate Theses and Dissertations

Microbial Induced Calcite Precipitation (MICP) is an attractive alternative for a variety geotechnical ground improvement practices commonly used today and has a variety of potential applications. This research focuses primarily on its use as a soil stabilization technique using the bacteria Sporosarcina Pasteurii and a single injection point percolation method adapted from previous research in granular soils. This method, and most published data, show an inherent variability in both physical and engineering properties due to the distribution of precipitated calcite within the specimen. The focus of this research is on the quantification of the variability in shear strength parameters induced …

Third-Party Services As Potential Sources For Law Enforcement Procurement Of Genomic Data, Katherine Kwong

Canadian Journal of Law and Technology

This paper examines two cases studies: Ancestry.com’s experience with law enforcement use of its genetic databases, and 23andMe’s responses to requests for users’ genomic data. Analyzing the responses to law enforcement requests by the two of the largest providers of third-party genomic services provides insight into the struggles third-party genomic services are likely to face and possible future directions for companies facing this type of law enforcement request.

Multiple States Of Nitrile Hydratase From Rhodococcus Equi Tg328-2: Structural And Mechanistic Insights From Electron Paramagnetic Resonance And Density Functional Theory Studies, Natalia Stein, Natalie Gumataotao, Natalia Hajnas, Rui Wu, Karunagala Pathiranage Wasantha Lankathilaka, Uwe Bornscheuer, Dali Liu, Adam T. Fiedler, Richard C. Holz, Brian Bennett

Physics Faculty Research and Publications

Iron-type nitrile hydratases (NHases) contain an Fe(III) ion coordinated in a characteristic “claw setting” by an axial cysteine thiolate, two equatorial peptide nitrogens, the sulfur atoms of equatorial cysteine-sulfenic and cysteine-sulfinic acids, and an axial water/hydroxyl moiety. The cysteine-sulfenic acid is susceptible to oxidation, and the enzyme is traditionally prepared using butyric acid as an oxidative protectant. The as-prepared enzyme exhibits a complex electron paramagnetic resonance (EPR) spectrum due to multiple low-spin (S = ¹/₂) Fe(III) species. Four distinct signals can be assigned to the resting active state, the active state bound to butyric acid, an …

An Equal Rights Amendment For The Twenty-First Century: Bringing Global Constitutionalism Home, Julie C. Suk

Articles

The last few years have seen a renewed push to constitutionalize sex equality in the United States. A generation after the federal Equal Rights Amendment (ERA) failed to be ratified by the requisite number of states, the ERA is on the platform of the 2017 Women's March on Washington. Oregon added a sex equality guarantee to its state constitution in 2014, joining 22 state constitutions and most constitutions around the world. Feminist coalitions, Hollywood celebrities, and members of Congress are vocally endorsing an ERA revival. Why is an ERA desired now, when judges have interpreted the Fourteenth Amendment to prohibit …

Towards A Continuous Assessment Of Cognitive Workload For Smartphone Multitasking Users, Angel Jimenez-Molina, Hernan Lira

H-Workload 2017: Models and Applications (Works in Progress)

The intermeshing of Smartphone interactions and daily activities depletes the availability of cognitive resources. This excessive demand may lead to several undesirable cognitive states, which can be avoided by continuously assessing the user cognitive workload. Recently, many attempts have emerged to assess this workload by using psycho physiological signals. This paper provides evidence that it is possible to train models that accurately identify in short time windows such cognitive workload by processing heart rate and blood oxygen saturation signals. This assessment could be applied in Smartphone notification delivery, interface adaptations or cognitive capabilities evaluation.