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2017

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The Roles Of Avr4 In Fungal Virulence,Cercosporin Biosynthesis And Its Potential Use In Host Induced Gene Silencing For Controlling Cercospora Leaf Blight Disease Of Soybeans, Josielle Santos Rezende Jan 2017

The Roles Of Avr4 In Fungal Virulence,Cercosporin Biosynthesis And Its Potential Use In Host Induced Gene Silencing For Controlling Cercospora Leaf Blight Disease Of Soybeans, Josielle Santos Rezende

LSU Doctoral Dissertations

The AVR4 effector, secreted by Cladosporium fulvum, has been demonstrated to be involved in pathogen virulence. Recent studies further demonstrated that Avr4 is highly conserved among several Cercospora species, indicating a potential important role of this gene in fungal virulence. Therefore, investigation to determine whether this fungal effector gene is present in Cercospora cf. flagellaris (previously known as C. kikuchii), the causal agent of soybean cercospora leaf blight (CLB) disease, and whether it plays any role in CLB disease development, is of great interest. In the present study, the Avr4 gene from C. cf. flagellaris was cloned and mutants lacking …

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The Effect Of Demographic And Clinical Factors On The Performance Of The Abbreviated Scoring Algorithm For The Baby And Infant Screen For Children With Autism Traits, Paige Elizabeth Cervantes Jan 2017

The Effect Of Demographic And Clinical Factors On The Performance Of The Abbreviated Scoring Algorithm For The Baby And Infant Screen For Children With Autism Traits, Paige Elizabeth Cervantes

LSU Doctoral Dissertations

Autism spectrum disorder (ASD) is a neurodevelopmental disorder characterized by deficits in social interaction and social communication as well as the presence of restricted, repetitive, and stereotyped patterns of behavior, interests, or activities (RRBIs; American Psychiatric Association [APA], 2013). Individuals with ASD experience lifelong and pervasive impairments across many domains of functioning. Early identification of ASD is imperative as earlier intervention is associated with greater gains in numerous areas. In regards to early identification efforts, routine screening is important. However, screening often fails to be conducted within early childhood care settings. To encourage greater rates of screening by offering a …

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Thermomechanical Modeling Of Polymerica Actuators, Qianxi Yang Jan 2017

Thermomechanical Modeling Of Polymerica Actuators, Qianxi Yang

LSU Doctoral Dissertations

In this dissertation, the application of smart polymers as actuators was investigated, with focuses on shape memory polymers and twisted-then-coiled artificial muscles. Thermomechanical models have been developed for various polymeric actuators, so as to facilitate interpretation of the underlying mechanisms and to provide guidance for future design. The classical one-way shape memory effect in amorphous shape memory polymers was first reproduced. The amorphous shape memory polymer was treated as a frozen-phase matrix with active-phase inclusions embedded in it. A phase evolution law was proposed from the physics perspective and the Mori-Tanaka approach was used to predict the effective mechanical properties. …

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The Effects Of Chemical Reagents And Physical Environment On The In Vitro And In Vivo Properties Of Adipose-Derived Multipotent Stromal Cells Isolated From Different Species, Wei Duan Jan 2017

The Effects Of Chemical Reagents And Physical Environment On The In Vitro And In Vivo Properties Of Adipose-Derived Multipotent Stromal Cells Isolated From Different Species, Wei Duan

LSU Doctoral Dissertations

Adult adipose derived mesenchymal stromal cells (ASCs) have been characterized in various species. Many factors may affect ASC fate and ASCs from different species may have different response to these factors. The first study was to identify the differences of the canine ASCs isolated from subcutaneous and infrapatellar adipose tissues, and evaluate the impact of cryopreservation on the cells. Based on paired comparisons of fresh and cryopreserved ASCs, cryopreserved ASCs had lower CD29 and CD44 protein expression and lower proliferation rates. The cryopreserved ASCs had relative lower mitochondria in the cytoplasm compared to the fresh ASCs regardless of tissue sources. …

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Fibroblast Growth Factor 21 Is A Novel Protein Sensor In Pregnancy, Elizabeth Frost Sutton Jan 2017

Fibroblast Growth Factor 21 Is A Novel Protein Sensor In Pregnancy, Elizabeth Frost Sutton

LSU Doctoral Dissertations

The twenty-first century has experienced a shift in cause of death worldwide from communicable diseases to noncommunicable diseases. Interestingly, many of these implicated chronic diseases, such as cancer, diabetes, and cardiovascular disease, have been shown to be programmed in the womb. As first posited by the Barker Hypothesis, adverse exposures in utero can increase an individual’s risk for chronic disease later in life. Therefore, pregnancy is an opportune time for intervention to improve the health of future generations. Studies of exposures known to negatively impact infant health, e.g. states of overnutrition (obesity, diabetes, excess gestational weight gain) and undernutrition (starvation, …

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Selected Works Of Richard Burchard: A Resource Guide, William Floyd Plummer Jan 2017

Selected Works Of Richard Burchard: A Resource Guide, William Floyd Plummer

LSU Doctoral Dissertations

This document serves as a resource guide for selected choral works of Richard Burchard, including pertinent background information and biographical details about the composer’s life, information concerning the compositional style and musical structure of his choral works, and accessibility and interpretive considerations for the choral conductor. Works were selected for this study chronologically by publication date, from Burchard’s first published work (Miserere mei, published 2009) through the beginning of this project in early 2012. Born in 1960, Richard Burchard is the Chair of the Music Department at Bellarmine University in Kentucky. Burchrd has written over 50 pieces, many of which …

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Human Gut Microbiota And Obesity: How Is Gut Microbiota Associated With Obesity Improvement Induced By Bariatric Surgeries Or Low-Calorie Diet Treatment?, Rui Zhang Jan 2017

Human Gut Microbiota And Obesity: How Is Gut Microbiota Associated With Obesity Improvement Induced By Bariatric Surgeries Or Low-Calorie Diet Treatment?, Rui Zhang

LSU Doctoral Dissertations

An increasing number of studies suggest that the complex microbial community of the human gut may have an important role in human health and disease conditions such as obesity and diabetes. However, the causal relationship among gut microbiota, obesity, weight loss, and the influence of bariatric surgery remains largely unknown. This study investigated the correlation between human gut microbiota and obesity via determining how gut microbiota is associated with obesity improvement induced by bariatric surgeries (BRS) and low-calorie diet (LCD) treatment. Specifically, we investigated the effects of various bariatric surgery (BRS) procedures and a low-calorie diet (LCD) treatment on the …

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Manipulating Physical Properties Of Complex Materials By Processing, Mohammad Saghayezhian Jan 2017

Manipulating Physical Properties Of Complex Materials By Processing, Mohammad Saghayezhian

LSU Doctoral Dissertations

Transition metal oxides (TMOs) are a class of materials in which charge, orbital, spin and lattice strongly interact with each other. In the present thesis, a special attention is paid to the physical properties of TMOs as a function of processing. First, TMOs are studied as substrates, where the surface is of prime importance. Second, the thin films are fabricated and their physical properties are presented. The SrTiO3 (001) and (111) surfaces are weakly and strongly polar and the free energy of intact surfaces should diverge. It is shown that in TiO2 terminated SrTiO3(001) the top most layer shows rumpling …

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Deterritorializing Dichotomies In Teacher Induction: A (Post)Ethnographic Study Of Un/Becoming An Elementary Science Teacher, Maria Ferris Greene Wallace Jan 2017

Deterritorializing Dichotomies In Teacher Induction: A (Post)Ethnographic Study Of Un/Becoming An Elementary Science Teacher, Maria Ferris Greene Wallace

LSU Doctoral Dissertations

Un/becoming an elementary science teacher is a dynamic phenomenon, yet the process is often intentionally limited to several taken-for-granted assumptions in research on science teacher induction. Inherent to research on beginning science teacher induction is also the construction of certain truths beginning science teachers, science teacher educators, and researchers think, feel, and live. This study complicates prevailing truths shaping notions of beginner, novice, induction, and traditions of inquiry as an ethicopolitical commitment to those implicated. In doing so, this study illuminates more expansive ways science teacher educators and those studying induction might study and understand the experiences of beginning science …

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Symbolic And Deep Learning Based Data Representation Methods For Activity Recognition And Image Understanding At Pixel Level, Manohar Karki Jan 2017

Symbolic And Deep Learning Based Data Representation Methods For Activity Recognition And Image Understanding At Pixel Level, Manohar Karki

LSU Doctoral Dissertations

Efficient representation of large amount of data particularly images and video helps in the analysis, processing and overall understanding of the data. In this work, we present two frameworks that encapsulate the information present in such data. At first, we present an automated symbolic framework to recognize particular activities in real time from videos. The framework uses regular expressions for symbolically representing (possibly infinite) sets of motion characteristics obtained from a video. It is a uniform framework that handles trajectory-based and periodic articulated activities and provides polynomial time graph algorithms for fast recognition. The regular expressions representing motion characteristics can …

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Nanoscale Studies Of Proteins And Thin Films Using Scanning Probe Microscopy, Zachary Lane Highland Jan 2017

Nanoscale Studies Of Proteins And Thin Films Using Scanning Probe Microscopy, Zachary Lane Highland

LSU Doctoral Dissertations

Nanostructures of organosilanes, thin metal films, and protein nanopatterns were prepared and analyzed with atomic force microscopy (AFM). Organosilanes with designed functional groups were used to selectively pattern green fluorescent protein at the nanoscale using protocols developed with particle lithography. Mesospheres are deposited onto a substrate to produce a surface mask. Organosilanes are deposited to form a matrix film surrounding nanopores for depositing proteins. The nanopatterns were characterized using AFM, after steps of particle lithography for directly visualizing surface changes. Studies with AFM also provide a compelling tool for teaching undergraduates to introduce concepts of nanoscience. An undergraduate laboratory was …

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A Model-Centric Framework For Advanced Operation Of Crystallization Processes, Navid Ghadipasha Jan 2017

A Model-Centric Framework For Advanced Operation Of Crystallization Processes, Navid Ghadipasha

LSU Doctoral Dissertations

Crystallization is the main physical separation process in many chemical industries. It is an old unit operation which can separate solids of high purity from liquids, and is widely applied in the production of food, pharmaceuticals, and fine chemicals. While industries in crystallization operation quite rely on rule-of-thumb techniques to fulfill their requirement, the move towards a scientific- and technological- based approach is becoming more important as it provides a mechanism for driving crystallization processes optimally and in more depth without increasing costs. Optimal operation of industrial crystallizers is a prerequisite these days for achieving the stringent requirements of the …

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The Rhetoric Of Hospitality: Conditions Of Death In America, Margaret Anne Callahan Jan 2017

The Rhetoric Of Hospitality: Conditions Of Death In America, Margaret Anne Callahan

LSU Doctoral Dissertations

The Right of Hospitality: Conditions of Death in America calls Western biomedicine’s approach to death into question. Death unifies all human experiences and is always possible, despite the human tendency to deny its existence and, instead, orient the self towards a futurity that is always out of reach. This project investigates the structures influencing how death in America occurs, and traces the roots of Western culture’s rejection of death to the execution by hemlock of Socrates’ immortalized in the Phaedo. Western biomedicine’s institutionalization of medicine requires that both patients and doctors enter into imbalanced hospitable relationships, and these pressures, along …

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The Role Of Defects In The Metal-Nonmetal Transition In Metallic Oxide Films, Gaomin Wang Jan 2017

The Role Of Defects In The Metal-Nonmetal Transition In Metallic Oxide Films, Gaomin Wang

LSU Doctoral Dissertations

Metal-nonmetal transition, or more specifically, metal-insulator transition (MIT) has been one of the most intriguing topics in condensed matter physics. Two theories describing fundamental driving mechanisms of MIT has been well-established over time: Mott-Hubbard theory and Anderson localization theory. The former mainly deals with contribution of electron interactions/correlations to the MIT, and the latter focuses on the role of disorder. However, it is an open topic how a system behaves when both effects exist in a system. This study mostly takes interest in a type of MIT induced by dimensionality-crossover in the transition metal oxides (TMOs) systems. TMO system is …

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Evolutionary History Of A Large-Genome Salamander Across Five Disjunct Regions Of The Southeastern United States, Catherine Elizabeth Newman Jan 2017

Evolutionary History Of A Large-Genome Salamander Across Five Disjunct Regions Of The Southeastern United States, Catherine Elizabeth Newman

LSU Doctoral Dissertations

The southeastern United States has a dynamic geologic history. Mountain range uplift, sea level fluctuations, and river basin evolution have shaped species geographic distributions in the region. My dissertation objectively identifies amphibian species with highly fragmented distributions in the Southeast (Chapter 2) and examines the phylogeographic history of one of those species, Plethodon serratus. This salamander species has a widely disjunct range across five regions: the Appalachians, the Ozarks, the Ouachitas, and two allopatric sites in Louisiana. Analyses of mitochondrial and nuclear loci (Chapter 3) showed that P. serratus is comprised of multiple genetic lineages, and the five regions are …

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Chemical Synthesis: New Methods For O-Glycosylation And The Preparation Of Organic Thin Films, Rashanique Deondria Quarels Jan 2017

Chemical Synthesis: New Methods For O-Glycosylation And The Preparation Of Organic Thin Films, Rashanique Deondria Quarels

LSU Doctoral Dissertations

This dissertation focuses on the diverse use of chemical synthesis. Herein, I will discuss the use of synthetic organic chemistry for the modification surfaces and the synthesis of small molecules. Chapter One is an introduction to the realm of surface chemistry. I have highlighted some key methods for surface modification. Additionally, methods to characterize the as-formed thin films are also outlined. In Chapter Two, I discuss the use of visible-light photoredox catalysis in the nanoscale lithography of Au(111) surfaces. Blue LED irradiation of solutions of NBDT in the presence of Ru(bpy)32+ results in the formation of p-nitrophenyl radicals that graft …

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Design, Synthesis, And Evaluation Of Innovative Bodipy-Peptidic Conjugates For Biological Application, Tyrslai Menyaee Williams Jan 2017

Design, Synthesis, And Evaluation Of Innovative Bodipy-Peptidic Conjugates For Biological Application, Tyrslai Menyaee Williams

LSU Doctoral Dissertations

Abstract Chapter 1 provides a brief introduction to colorectal cancer (CRC), methods of detection, photodynamic therapy (PDT), and EGFR as an attractive targeting strategy for imaging CRC. Chapter 2 details the synthesis of ten peptide sequences for targeting EGFR receptors at the site of CRC, as a collaborative work with Dr. Seetharama Jois. This chapter also investigates the efficacy of these peptides for EGFR. Chapter 3 offers the first synthetic strategy for developing BODIPY-peptide conjugates via amide linkages. All conjugates were optimized for the best reaction conditions and good yields were observed. Each conjugate was subjected to photophysical, SPR, and …

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The Fooding Of Narrative In Contemporary Women's Transnational Fiction, Stacey Marie Amo Jan 2017

The Fooding Of Narrative In Contemporary Women's Transnational Fiction, Stacey Marie Amo

LSU Doctoral Dissertations

The Fooding of Narrative in Contemporary Women’s Transnational Fiction examines how foodstuffs are strategically deployed in recent fiction about migration to the United States. The novels examined, all set in the period following the passage of the Immigration and Nationality Act of 1965, depict the experience of resettlement from a diverse set of postcolonial sites: Haiti, Cuba, Vietnam, and India. The Fooding of Narrative argues that food is deployed as a structuring trope and acquires narrative significance in articulating the profound sense of identity crisis that frequently accompanies the immigration experience. By focusing on food’s narrative significance in a broad …

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Elephants, Local Livelihoods, And Landscape Change In Tsavo, Kenya., Peter Ngugi Kamau Jan 2017

Elephants, Local Livelihoods, And Landscape Change In Tsavo, Kenya., Peter Ngugi Kamau

LSU Doctoral Dissertations

This dissertation provides a historical context for socio-ecological relationships in Tsavo, Kenya by focusing on the interaction between elephants and people in the landscape. A better understanding of the relationship between elephants and people in the Tsavo landscape promotes opportunities for better policy outcomes. The dissertation engages with the analytical approach of political ecology, which has enabled it to provide a more nuanced understanding of the relationship between elephants and people in Tsavo. Apolitical accounts of human-elephant conflicts in Tsavo do not adequately address the colonial roots of human-elephant conflicts or their consequences for local livelihoods. This dissertation demonstrates how …

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Finding Voice In The Academy Through Yellow Storytelling: An Asian American Autoethnography On Suffering In Higher Education, Thuong Thi Nguyen Jan 2017

Finding Voice In The Academy Through Yellow Storytelling: An Asian American Autoethnography On Suffering In Higher Education, Thuong Thi Nguyen

LSU Doctoral Dissertations

ABSTRACT Higher education institutions in the United States (U.S.) were founded for a select segment of the population, i.e. white Christian men from upper socioeconomic classes (Thelin, 2011). Research shows the policies, pedagogies, and practices created for use within majority populations and dominant cultures are not as beneficial or effective for individuals from underrepresented backgrounds. As the U.S. becomes more diverse and college enrollment among diverse students increases, higher education institutions need to identify more holistic approaches and investigate alternative methods to better serve these populations. This dissertation is a response to that need. In order to offer other alternatives, …

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Effect Of Metal Speciation In Fly Ash On Environmentally Persistent Free Radical Formation, Elisabeth Eileen Cook Jan 2017

Effect Of Metal Speciation In Fly Ash On Environmentally Persistent Free Radical Formation, Elisabeth Eileen Cook

LSU Doctoral Dissertations

Waste incineration and Superfund sites lead to the formation of pollutants harmful to both the environment and human health. Environmentally Persistent Free Radicals (EPFRs) are a class of newly discovered radical pollutants known to form on combustion by-products such as fly ash and particulate matter. EPFRs are rapidly gaining attention for their harmful effects on the environment and human health. Previous research has shown the formation of EPFRs through surface-mediated reactions with transition metal-oxides on particulates. The work presented in this dissertation explores the relationship between fly ash composition and EPFR formation. Fly ash production occurs from combustion systems, namely …

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Motivation For Tobacco Cessation Among Nicotine Dependent Postmenopausal Females, Mackenzie Rae Peltier Jan 2017

Motivation For Tobacco Cessation Among Nicotine Dependent Postmenopausal Females, Mackenzie Rae Peltier

LSU Doctoral Dissertations

Postmenopausal females continue to smoke despite considerable health risks related to low levels of estrogen in combination with antiestrogenic effects of nicotine. These females face barriers to cessation that are more severe than their male and pre-menopausal counterparts. These barriers include negative affect, weight concerns, and menopausal symptom severity. Brief motivation-based interventions (B-MIs) that incorporate individualized health-related feedback have demonstrated efficacy for smoking cessation, but have not been tested among postmenopausal females. The current study explored the effect of negative affect, weight concerns, and menopausal symptom severity on motivation and readiness to quit smoking, and the effectiveness of a B-MI …

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Aleksander Peçi Before And After The Iron Curtain, Elida Dakoli Jan 2017

Aleksander Peçi Before And After The Iron Curtain, Elida Dakoli

LSU Doctoral Dissertations

Abstract This research describes the life, compositional style and works of one of the most well-known Albanian composers, Aleksander Peçi, focusing on how the political climate affected his life and his art. Several of Peçi’s techniques are examined, including Carousel structure, Cartesian Production, Kabaoisos, and Polygravité. Albanian iso-polyphony and its influence on Peçi’s work are discussed. The research was done in hopes of reaching a better understanding of the effects Communism and the subsequent period of freedom have had on Albanian music.

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Epigenetic Profiling Of Mammalian Retrotransposons, Arundhati Bakshi Jan 2017

Epigenetic Profiling Of Mammalian Retrotransposons, Arundhati Bakshi

LSU Doctoral Dissertations

Over evolutionary time, mammalian genomes have accumulated a large number of retrotransposons, making up about half of the genome in any given species. These retrotransposons are typically repressed by epigenetic mechanisms, one of the main ones being DNA methylation. It is well known that improper DNA methylation of retrotransposons can have unwanted consequences on nearby gene expression, and hypomethylation of retrotransposons has been frequently observed in various cancers. Nevertheless, it has been notoriously difficult to study retrotransposon loci individually due to the highly repetitive nature of their sequences. To address this issue, we have developed a novel protocol termed HT-TREBS …

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Determining The Influence Of Imidacloprid And Glufosinate Ammonium On The Population Dynamics Of Twospotted Spider Mite Populations In Louisiana Cotton, Sebe Anthony Brown Jan 2017

Determining The Influence Of Imidacloprid And Glufosinate Ammonium On The Population Dynamics Of Twospotted Spider Mite Populations In Louisiana Cotton, Sebe Anthony Brown

LSU Doctoral Dissertations

Twospotted spider mites, Tetranychus urticae, is an important agricultural pest of many field crops worldwide. This study investigated the impacts of imidacloprid seed treatments on populations of twospotted spider mites while also investigating if exogenous applications of jasmonic acid can offset any hormone modulating effects caused by seed treatments. Imidacloprid seed treatments significantly increased cumulative adult mite days in 2013 but not 2015 or 2016 in the field. Applications of 10 millimolar jasmonic acid did not reduce mite severity or injury in all field trials. Imidacloprid seed treatments significantly increased all spider mite life stages in the laboratory while applications …

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Structure-Based Prediction Of Protein-Protein Interaction Networks Across Proteomes, Surabhi Maheshwari Jan 2017

Structure-Based Prediction Of Protein-Protein Interaction Networks Across Proteomes, Surabhi Maheshwari

LSU Doctoral Dissertations

Protein-protein interactions (PPIs) orchestrate virtually all cellular processes, therefore, their exhaustive exploration is essential for the comprehensive understanding of cellular networks. Significant efforts have been devoted to expand the coverage of the proteome-wide interaction space at molecular level. A number of experimental techniques have been developed to discover PPIs, however these approaches have some limitations such as the high costs and long times of experiments, noisy data sets, and often high false positive rate and inter-study discrepancies. Given experimental limitations, computational methods are increasingly becoming important for detection and structural characterization of PPIs. In that regard, we have developed a …

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Succinct Data Structures For Parameterized Pattern Matching And Related Problems, Arnab Ganguly Jan 2017

Succinct Data Structures For Parameterized Pattern Matching And Related Problems, Arnab Ganguly

LSU Doctoral Dissertations

Let T be a fixed text-string of length n and P be a varying pattern-string of length |P| <= n. Both T and P contain characters from a totally ordered alphabet Sigma of size sigma <= n. Suffix tree is the ubiquitous data structure for answering a pattern matching query: report all the positions i in T such that T[i + k - 1] = P[k], 1 <= k <= |P|. Compressed data structures support pattern matching queries, using much lesser space than the suffix tree, mainly by relying on a crucial property of the leaves in the tree. Unfortunately, in many suffix tree variants (such as parameterized suffix tree, order-preserving suffix tree, and 2-dimensional suffix tree), this property does not hold. Consequently, compressed representations of these suffix tree variants have been elusive. We present the first compressed data structures for two important variants of the pattern matching problem: (1) Parameterized Matching -- report a position i in T if T[i + k - 1] = f(P[k]), 1 <= k <= |P|, for a one-to-one function f that renames the characters in P to the characters in T[i,i+|P|-1], and (2) Order-preserving Matching -- report a position i in T if T[i + j - 1] and T[i + k -1] have the same relative order as that of P[j] and P[k], 1 <= j < k <= |P|. For each of these two problems, the existing suffix tree variant requires O(n*log n) bits of space and answers a query in O(|P|*log sigma + occ) time, where occ is the number of starting positions where a match exists. We present data structures that require O(n*log sigma) bits of space and answer a query in O((|P|+occ) poly(log n)) time. As a byproduct, we obtain compressed data structures for a few other variants, as well as introduce two new techniques (of independent interest) for designing compressed data structures for pattern matching.

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Activity Of Benzobicyclon Herbicide In Common Louisiana Rice Production Practices, Benjamin M. Mcknight Jan 2017

Activity Of Benzobicyclon Herbicide In Common Louisiana Rice Production Practices, Benjamin M. Mcknight

LSU Doctoral Dissertations

A study was conducted at the LSU AgCenter H. Rouse Caffey Rice Research Station (RRS) to evaluate benzobicyclon herbicide application timing on water-seeded rice. Benzobicyclon was applied at seven different timings, at 246 g ai ha-1 in a water-seeded rice production system. Benzobicyclon controlled ducksalad above 90% when applied into the pinpoint flood. Barnyardgrass control was greater than 90% from applications made on pegging rice and immediately following pinpoint flood establishment. At 49 days after treatment (DAT), yellow nutsedge control exceeded 90% following benzobicyclon treatment with a pegging rice or pinpoint flood timing. Ducksalad control was greater than 90% following …

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Epigenetic Response Of Imprinted Domains During Carcinogenesis, Corey Lane Bretz Jan 2017

Epigenetic Response Of Imprinted Domains During Carcinogenesis, Corey Lane Bretz

LSU Doctoral Dissertations

The first part of this work induced T-cell lymphoma in mice by employing a breeding scheme involving mouse strains expressing the KrasG12D oncoprotein and mice expressing cyclic recombinase from the mouse mammary tumor virus promoter. Imprinted domains were then systematically surveyed for DNA methylation changes during tumor progression using combined bisulfite restriction analysis and next-generation-bisulfite-sequencing. Hyper-or hypomethylation was detected at the imprinting control regions (ICRs) of the Dlk1, Peg10, Peg3, Grb10 and Gnas domains. These DNA methylation changes at ICRs were more prevalent and consistent than those observed at the promoter regions of well-known tumor suppressors, such as Mgmt, Fhit …

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The Formation Of Polycyclic Aromatic Hydrocarbons From The Pyrolysis Of Model 1-Alkene Fuels, Eva Christine Caspary Jan 2017

The Formation Of Polycyclic Aromatic Hydrocarbons From The Pyrolysis Of Model 1-Alkene Fuels, Eva Christine Caspary

LSU Doctoral Dissertations

To understand the role of 1-alkenes and allylic radicals in the reaction pathways leading to the formation and growth of polycyclic aromatic hydrocarbons (PAH), pyrolysis experiments have been performed with three 1-alkene fuels—propylene (CH₂=CH–CH₃), 1-butene (CH₂=CH–CH₂–CH₃), and 1-pentene (CH₂=CH–CH₂–CH₂–CH₃)—at temperatures of 600 – 1000 °C and a fixed residence time of 0.31 s. The experiments are carried out in an isothermal laminar-flow quartz-tube reactor. Analyses of the pyrolysis products by gas-chromatographic and high-pressure liquid-chromatographic techniques reveal that the three fuels differ in: 1) their conversion behavior, 2) the relative amounts of the major C₂ – C₄ species produced, and 3) …

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