Digital Commons Network^™

Antibiotic Resistance Genes In Minnesota Soil Bacteria From Areas Of High And Low Ferric Iron, Gunner L. Drossel, Presley Martin Phd

Departmental Honors Projects

Naturally-occurring antibiotic resistance genes in soil bacteria represent a potentially important reservoir of genes that could contribute to antibiotic resistance of human pathogens. It has been reported that over 40 genes in bacterial genomes are controlled by concentrations of ferric iron. We examined the effect of soil metal content on the level of resistance to two antibiotics, ampicillin (Amp) and tetracycline (Tet), and the presence of multiple genes that code for efflux pump-mediated resistance. These pumps act to export toxins (e.g. heavy metals and antibiotics, perhaps). Because of this, growth in heavy metal-contaminated soils might select for antibiotic resistance. Ninety-six …

Child Support Collections To Offset Out Of Home Placement Costs: A Study Of Cost Effectiveness, Trish Skophammer

School of Business Student Theses and Dissertations

Families experiencing out-of-home placement (OHP) are in crisis. The fact that a child has been removed from the home means the family has become involved with the child welfare or juvenile justice system. Families that experience OHP are disproportionately poor, and the reasons for OHP often stem from poverty. Because OHP is expensive, and society values parental responsibility, federal and state laws require that parents be referred to the child support system to help offset the cost of OHP through child support collections. This study explores practices around OHP cases in the child support system and adds to the small …

Legacy Of Women And The Culture Of An Organization, Nicole C. Kutsi

School of Business Student Theses and Dissertations

The purpose of this paper is to explore women in executive leadership positions in traditionally male-dominated public-sector organizations. This research will determine whether women entering into these positions have the ability to change the culture of a traditionally male-dominated institution so that women are regarded as equals to men in leadership success, team-building, solicitation of organizational input from subordinates, and overall operational efficiency in the organization. The study will also explore how uniquely feminine qualities contribute to a distinctively transformational leadership approach in women, challenging the common notion that only men are qualified to lead because of their traditionally aggressive …

Redrawing The Boundaries Of Relational Crime, Cynthia Godsoe

Faculty Scholarship

No abstract provided.

Novel Hla-Dp Region Susceptibility Loci Associated With Severe Acute Gvhd., Rakesh K. Goyal, S J. Lee, T Wang, M Trucco, M Haagenson, S R. Spellman, M Verneris, R E. Ferrell

Manuscripts, Articles, Book Chapters and Other Papers

Despite HLA allele matching, significant acute GvHD remains a major barrier to successful unrelated donor BMT. We conducted a genome-wide association study (GWAS) to identify recipient and donor genes associated with the risk of acute GvHD. A case-control design (grade III-IV versus no acute GvHD) and pooled GWA approach was used to study European-American recipients with hematological malignancies who received myeloablative conditioning non-T-cell-depleted first transplantation from HLA-A, -B, -C, -DRB1, -DQB1 allele level (10/10) matched unrelated donors. DNA samples were divided into three pools and tested in triplicate using the Affymetrix Genome-wide SNP Array 6.0. We identified three novel susceptibility …

Visualization Of Molecular Structures Using Hololens-Based Augmented Reality., M A Hoffman, J B Provance

Manuscripts, Articles, Book Chapters and Other Papers

Biological molecules and biologically active small molecules are complex three dimensional structures. Current flat screen monitors are limited in their ability to convey the full three dimensional characteristics of these molecules. Augmented reality devices, including the Microsoft HoloLens, offer an immersive platform to change how we interact with molecular visualizations. We describe a process to incorporate the three dimensional structures of small molecules and complex proteins into the Microsoft HoloLens using aspirin and the human leukocyte antigen (HLA) as examples. Small molecular structures can be introduced into the HoloStudio application, which provides native support for rotating, resizing and performing other …

The Privacy, Probability, And Political Pitfalls Of Universal Dna Collection, Meghan J. Ryan

SMU Science and Technology Law Review

Watson and Crick’s discovery of the structure of DNA (deoxyribonucleic acid) in 1953 launched a truth-finding mission not only in science but also in the law. Just thirty years later–after the science had evolved–DNA evidence was being introduced in criminal courts. Today, DNA evidence is heavily relied on in criminal and related cases. It is routinely introduced in murder and rape cases as evidence of guilt; DNA databases have grown as even arrestees have been required to surrender DNA samples; and this evidence has been used to exonerate hundreds of convicted individuals. DNA evidence is generally revered as the “gold …

Patents, Industrial Designs, And The Trans-Pacific Partnership: Articles 18.37–18.46 And 18.55–18.56, David O. Taylor, Aaron Pirouznia

SMU Science and Technology Law Review

This essay summarizes the articles of the Trans-Pacific Partnership dealing with patents and industrial designs, and compares and contrasts those articles with U.S. law and the Agreement on Trade-Related Aspects of Intellectual Property Rights (TRIPS).

A Preface To The Philosophy Of Legal Information, Kevin P. Lee

SMU Science and Technology Law Review

No abstract provided.

Much Ado About The Tpp’S Effect On Pharmaceuticals, Emily Michiko Morris

SMU Science and Technology Law Review

No abstract provided.

Appendectomy Versus Non-Operative Treatment For Acute Uncomplicated Appendicitis In Children: Study Protocol For A Multicentre, Open-Label, Non-Inferiority, Randomised Controlled Trial., Nigel J. Hall, Simon Eaton, Olivier Abbo, Alexis P. Arnaud, Marianne Beaudin, Mary Brindle, Andreana Bütter, Dafydd Davies, Tim Jancelewicz, Kathy Johnson, Richard Keijzer, Eveline Lapidus-Krol, Martin Offringa, Nelson Piché, Risto Rintala, Erik Skarsgard, Jan F. Svensson, Wendy J. Ungar, Tomas Wester, Andrew R. Willan, Augusto Zani, Shawn D. St Peter, Agostino Pierro

Manuscripts, Articles, Book Chapters and Other Papers

Background: Appendectomy is considered the gold standard treatment for acute appendicitis. Recently the need for surgery has been challenged in both adults and children. In children there is growing clinician, patient and parental interest in non-operative treatment of acute appendicitis with antibiotics as opposed to surgery. To date no multicentre randomised controlled trials that are appropriately powered to determine efficacy of non-operative treatment (antibiotics) for acute appendicitis in children compared with surgery (appendectomy) have been performed.

Methods: Multicentre, international, randomised controlled trial with a non-inferiority design. Children (age 5-16 years) with a clinical and/or radiological diagnosis of acute uncomplicated appendicitis …

Yap And The Hippo Pathway In Pediatric Cancer., Atif Ahmed, Abdalla D. Mohamed, Melissa Gener, Weijie Li, Eugenio Taboada

Manuscripts, Articles, Book Chapters and Other Papers

The Hippo pathway is an important signaling pathway that controls cell proliferation and apoptosis. It is evolutionarily conserved in mammals and is stimulated by cell-cell contact, inhibiting cell proliferation in response to increased cell density. During early embryonic development, the Hippo signaling pathway regulates organ development and size, and its functions result in the coordinated balance between proliferation, apoptosis, and differentiation. Its principal effectors, YAP and TAZ, regulate signaling by the embryonic stem cells and determine cell fate and histogenesis. Dysfunction of this pathway contributes to cancer development in adults and children. Emerging studies have shed light on the upregulation …

Multiple Targets For Novel Therapy Of Fsgs Associated With Circulating Permeability Factor., Virginia J. Savin, Mukut Sharma, Jianping Zhou, David Genochi, Ram Sharma, Tarak Srivastava, Amna Ilahe, Pooja Budhiraja, Aditi Gupta, Ellen T. Mccarthy

Manuscripts, Articles, Book Chapters and Other Papers

A plasma component is responsible for altered glomerular permeability in patients with focal segmental glomerulosclerosis. Evidence includes recurrence after renal transplantation, remission after plasmapheresis, proteinuria in infants of affected mothers, transfer of proteinuria to experimental animals, and impaired glomerular permeability after exposure to patient plasma. Therapy may include decreasing synthesis of the injurious agent, removing or blocking its interaction with cells, or blocking signaling or enhancing cell defenses to restore the permeability barrier and prevent progression. Agents that may prevent the synthesis of the permeability factor include cytotoxic agents or aggressive chemotherapy. Extracorporeal therapies include plasmapheresis, immunoadsorption with protein A …

Biochemical And Biophysical Methods For Analysis Of Poly(Adp-Ribose) Polymerase 1 And Its Interactions With Chromatin., Maggie H. Chassé, Uma M. Muthurajan, Nicholas J. Clark, Michael A. Kramer, Srinivas Chakravarthy, Thomas Irving, Karolin Luger

Manuscripts, Articles, Book Chapters and Other Papers

Poly (ADP-Ribose) Polymerase I (PARP-1) is a first responder to DNA damage and participates in the regulation of gene expression. The interaction of PARP-1 with chromatin and DNA is complex and involves at least two different modes of interaction. In its enzymatically inactive state, PARP-1 binds native chromatin with similar affinity as it binds free DNA ends. Automodification of PARP-1 affects interaction with chromatin and DNA to different extents. Here we describe a series of biochemical and biophysical techniques to quantify and dissect the different binding modes of PARP-1 with its various substrates. The techniques listed here allow for high …

Predictors Of Loss To Follow-Up Among Children With Type 2 Diabetes., Ashley Shoemaker, Peiyao Cheng, Robin L. Gal, Craig Kollman, William V. Tamborlane, Georgeanna J. Klingensmith, Mark A. Clements, Tamara S. Hannon, Rubina Heptulla, Joane Less, Jamie Wood, Pediatric Diabetes Consortium

Manuscripts, Articles, Book Chapters and Other Papers

BACKGROUND/AIMS: Youth with type 2 diabetes (T2D) have poor compliance with medical care. This study aimed to determine which demographic and clinical factors differ between youth with T2D who receive care in a pediatric diabetes center versus youth lost to follow-up for >18 months.

METHODS: Data were analyzed from 496 subjects in the Pe-diatric Diabetes Consortium registry. Enrollment variables were selected a priori and analyzed with univariable and multivariable logistic regression models.

RESULTS: After a median of 1.3 years from enrollment, 55% of patients were lost to follow-up. The final model included age, race/ethnicity, parent education, and estimated distance to …

The Adolescent Substance Use Risk Continuum: A Cultural, Strengths-Based Approach To Case Conceptualization, Alexis Miller, Jennifer M. Cook

College of Education Faculty Research and Publications

Many theories are used to conceptualize adolescent substance use, yet none adequately assist mental health professionals in assessing adolescents’ strengths and risk factors while incorporating cultural factors. The authors reviewed common adolescent substance abuse theories and their strengths and limitations, and offer a new model to conceptualize adolescent substance use: The Adolescent Substance Use Risk Continuum. We posit that this strengths-based continuum enables clinicians to decrease stigma and offer hope to adolescents and their caregivers, as it integrates relevant factors to strengthen families and minimize risk. This model is a tool for counselors to use as they conceptualize client cases, …

International Consensus (Icon) On: Clinical Consequences Of Mite Hypersensitivity, A Global Problem., Mario Sánchez-Borges, Enrique Fernandez-Caldas, Wayne R. Thomas, Martin D. Chapman, Bee Wah Lee, Luis Caraballo, Nathalie Acevedo, Fook Tim Chew, Ignacio J. Ansotegui, Leili Behrooz, Wanda Phipatanakul, Roy Gerth Van Wijk, Demoly Pascal, Nelson Rosario, Motohiro Ebisawa, Mario Geller, Santiago Quirce, Susanne Vrtala, Rudolf Valenta, Markus Ollert, Giorgio Walter Canonica, Moises A. Calderón, Charles S. Barnes, Adnan Custovic, Suwat Benjaponpitak, Arnaldo Capriles-Hulett

Manuscripts, Articles, Book Chapters and Other Papers

Since mite allergens are the most relevant inducers of allergic diseases worldwide, resulting in significant morbidity and increased burden on health services, the International Collaboration in Asthma, Allergy and Immunology (iCAALL), formed by the American Academy of Allergy, Asthma and Immunology (AAAAI), the American College of Allergy, Asthma and Immunology (ACAAI), the European Academy of Allergy and Clinical Immunology (EAACI), and the World Allergy Organization (WAO), has proposed to issue an International Consensus (ICON) on the clinical consequences of mite hypersensitivity. The objectives of this document are to highlight aspects of mite biology that are clinically relevant, to update the …

Immunodeficiency-Associated Lymphoid Hyperplasia As A Cause Of Intussusception In A Case Of Activated Pi3k-Δ Syndrome., Daniel Mettman, Isabelle Thiffault, Chitra Dinakar, Carol J. Saunders

Manuscripts, Articles, Book Chapters and Other Papers

Activated PI3K-δ syndrome refers to a recently described primary immunodeficiency syndrome consisting of recurrent sinopulmonary infections, lymphadenopathy, mucosal lymphoid aggregates, increased susceptibility to Epstein-Barr virus and cytomegalovirus, and increased incidence of B-cell lymphomas. Variants in PIK3CD, which encodes the phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit delta isoform, enhance membrane association and kinase activity, resulting in increased signal transduction through the PI3K-Akt pathway. Whole-exome sequencing revealed a pathogenic PIK3CD variant in a patient with history of immunologic impairment, recurrent sinopulmonary infections, and lymphoid hyperplasia presenting as intussusception. This case illustrates that while lymphoid hyperplasia secondary to immunodeficiency is most often unsurprising and …

A Vast Injustice: The Public Debate And Legislative Battle Over Compulsory Eugenic Sterilization In Louisiana, 1924 -- 1932, Adelaide Hair Barr

LSU Doctoral Dissertations

From 1924 to 1932, Louisiana lawmakers considered five bills that would have granted superintendents of state institutions and some private hospitals the authority to forcibly sterilize their patients. Based on similar legislation passed in thirty-six other states, the bills cited eugenics as evidence that stripping these patients of their ability to reproduce would prevent the conditions such as feeblemindedness from passing on to the next generation. Although none of the bills passed both houses of the Louisiana legislature, a couple of them came dangerously close to becoming law. The debate among legislators, professionals, and social reformers provides a greater understanding …

Patterns Of Diversification In A Neotropical Radiation Of Birds (Aves: Furnariidae), Glenn F. Seeholzer

LSU Doctoral Dissertations

Ecology and the role of natural selection in lineage diversification has been a central topic in evolutionary biology since Darwin. At the macroevolutionary scale, this idea is embodied in the ecological theory of adaptive radiation, which posits that rapid diversification is driven by ecological adaptive radiation in which speciation is coupled with niche divergence. Within species, the theory of ecological speciation proposes that local adaptation drives speciation by reducing gene flow among populations occupying different environments either by directly reducing migration or by reducing the fitness of migrants. Much progress has been made testing these predictions in a multitude of …

The Immediate And Extended Effect Of Diet And Exercise On Metabolic Flexibility, Timothy Allerton

LSU Doctoral Dissertations

Metabolic inflexibility is an emerging physiological marker indicative of metabolic dysfunction and associated with type 2 diabetes (T2D) and obesity. Exercise is a potent stimulus to improve metabolic health, however, not much is known about the acute effects of exercise on metabolic flexibility (METFLEX). The purpose of this dissertation was study the time-course of diet and exercise and its effects on METFLEX. Three studies were conducted to investigate molecular and clinical aspects of diet and exercise and how these stimuli may alter the metabolic response to fuel. The first study was conducted on obesity-prone, Osborne-Mendel (OM) and obesity-resistant S5B/Pl (S5B) …

Hybrid Simulation-Based Planning Framework For Agri-Fresh Produce Supply Chain, Mohammed Mesabbah

Doctoral

The ever-increasing demand for fresh and healthy products raises the economic importance of managing Agri-Fresh Produce Supply Chain (AFPSC) effectively. However, the literature review has indicated that many challenges undermine efficient planning for AFPSCs. Stringent regulations on production and logistics activities, production seasonality and high yield variations (quantity and quality), and products vulnerability to multiple natural stresses, alongside with their critical shelf life, impact the planning process. This calls for developing smart planning and decision-support tools which provides higher efficiency for such challenges. Modelling and simulation (M&S) approaches for AFPSC planning problems have a proven record in offering safe and …

A Multiple Case Narrative Examining The Experiences Of Alumni Of A Minority Research And Training Program, Gretchen Schneider Burton

LSU Doctoral Dissertations

The implementation of minority research and training (MRT) programs at the post secondary level has risen to close the educational achievement gap and build undergraduate pathways to generate a highly skilled and diverse STEM pool. Although the numbers of advanced science related degrees have slowly increased over the past decade, partially due to the utilization of MRT programs, this increase has not been adequate to establish an impactful representation in either academic or industry research careers. Numerous studies identify various support interventions influencing positive outcomes among MRT participants achieving graduate degrees. However, majority of the studies utilize quantitative or a …

The Effects Of Aerobic Exercise And Lipolytic Stimulation On Skeletal Muscle And Adipose Expression Of Perilipin 3 (Plin3) In Individuals With Type 2 Diabetes And Women With Polycystic Ovary Syndrome, Jeffrey Daniel Covington

LSU Doctoral Dissertations

Ectopic lipid accumulation in skeletal muscle is linked with insulin resistance and type 2 diabetes (T2D). Neutral lipid is stored as lipid droplets, which are coated by lipid droplet coat proteins. One important class of these proteins are the perilipins. Little research has focused on perilipin 3 (PLIN3) and coatomer gtp-ases, theorized to be involved in PLIN3 directed lipid oxidation. Under lipolytic stimulation with epinephrine or palmitate, forskolin, and ionomysin (PFI), primary human myotubes taken from health non-T2D donors showed increased protein content levels of PLIN3. Concordantly, following endurance exercise in non-T2D males—an experimental paradigm shown to increase lipolysis—levels of …

Functional And Structural Analyses Of A Bacterial Antifreeze Protein, Chen Wang

LSU Doctoral Dissertations

Antifreeze proteins (AFPs) are a class of ice binding proteins (IBPs) that are expressed by different cold-adapted organisms to increase their freezing tolerance. AFPs have two major properties: thermal hysteresis and ice recrystallization inhibition. Here we report the functional and structural analyses of a bacterial AFP, IBPv. IBPv was originally secreted by a bacterium recovered from a deep glacial ice core drilled at Vostok Station, Antarctica. Our study showed that the recombinant protein rIBPv exhibited a thermal hysteresis of 2°C at concentrations higher than 50 µM, effectively inhibited ice recrystallization, and enhanced bacterial viability during freeze-thaw cycling. Circular dichroism scans …

Assessing The Effects Of The Smartphone As A Learning Tool On The Academic Achievement And Motivation Of High School Agriculture Students In Louisiana, Henry Eric Smith

LSU Doctoral Dissertations

Perhaps the most influential device in modern society is the smartphone. Over 90% of Americans aged 18-29 own a smartphone and 74% of teenagers reported using a smartphone as their primary internet connection. Students perceived that using smartphones in the classroom aided learning. However, two-thirds of American high schools ban students from using phones in the classroom. Secondary science curriculum focuses on subjects that regard the biodiversity of plant and animal species, but disregard the student’s ability to identify species. Consequently, secondary students in general are very poor at identifying species of trees. Previous research supports the idea that advanced …

Lignocellulosic Syrup Production From Energy Cane Bagasse And Fumaric Acid Fermentation, Fang Deng

LSU Doctoral Dissertations

Lignocellulosic syrup from dilute ammonia pretreated energy cane bagasse is a promising fermentable sugar feedstock that can improve the logistics associated with long-distance transportation, long-time storage, and year-round supply of lignocellulosic biomass to processing industries. However, non-sugar compounds such as organic acids, furans and phenolic compounds formed during pretreatment can have negative effects on downstream processes such as fermentation. This dissertation focused on addressing challenges associated with the bioconversion of energy cane bagasse to syrup and its fermentation to fumaric acid. The first goal (Chapter 2) was to evaluate the removal of non-sugar compounds with minimal sugar losses from dilute …

Characterization Of Dehalogenimonas Strains From A Louisiana Superfund Site That Reductively Dehalogenate Polychlorinated Alkanes, Trent Anthony Key

LSU Doctoral Dissertations

The research described in this dissertation was aimed at expanding understanding of the diversity and functional roles of microorganisms able to anaerobically transform chlorinated compounds. During the course of the research, a novel strictly anaerobic bacterial isolate originating from chlorinated solvent contaminated groundwater at a Superfund Site in south Louisiana (USA) was characterized. Phylogenetic analysis based on 16S rRNA gene sequencing placed the newly isolated strain, designated as NSZ-14 T, within the genus Dehalogenimonas but within a separate lineage from previously described species. Like previously described species within the genus Dehalogenimonas (i.e., D. lykanthroporepellens and D. alkenigignens), strain NSZ-14T was …

Improved Protein Characterizations Using Ionic Liquids: Page And Maldi-Ms, Punprabhashi Vidanapathirana

LSU Doctoral Dissertations

Protein separation by polyacrylamide gel electrophoresis (PAGE) and identification by matrix assisted laser desorption ionization-mass spectrometry (MALDI-MS) are primary tools of protein analysis. In these techniques, surfactants are used in protein sample preparation in order to enhance the protein solubility. Conventional surfactants have shown limitations in protein analysis due to the structural complexity of proteomes, resulting in low resolution. The research goal of this dissertation is to address some of these limitations by applying novel cationic ionic liquid surfactants (ILS), N-alkyl-4-methyl pyridinium bromide (CnPBr where n=4, 8, 11). The ILS would be suitable candidates to be used in PAGE protein …

Gender Bias In Leadership: Do Gender Of Leader, Type Of Error, Diversity Climate, And Gender Of Subordinate Affect Faculty Perceptions Of Academic Leaders' Effectiveness?, Adriana Alfaro

LSU Doctoral Dissertations

The cyphers don’t lie: women remain significantly underrepresented in positions of authority across the United States, despite their nearly equal representation in the labor force. Gender bias has been proposed as one of the major reasons for the disparity in leadership roles between men and women. Therefore, the primary purpose of this study was to investigate whether gender bias influenced faculty’s perceptions of leadership effectiveness at a Research 1 (R1) Doctoral institution in the southeastern United States when high-ranking university leaders made mistakes, and how type of error, diversity climate, and gender of subordinate affected this relationship. Two scenarios were …