Digital Commons Network^™

Discovery And Fine-Mapping Of Adiposity Loci Using High Density Imputation Of Genomewide Association Studies In Individuals Of African Ancestry: African Ancestry Anthropometry Genetics Consortium, Mary F. Feitosa, Mary K. Wojczynski, D. C. Rao, Ingrid B. Borecki, Et Al.

Open Access Publications

No abstract provided.

An Optimized, Broadly Applicable Piggybac Transposon Induction System, Zongtai Qi, Michael Nathaniel Wilkinson, Xuhua Chen, Sumithra Sankararaman, David Mayhew, Robi David Mitra

Open Access Publications

No abstract provided.

The Expanding Toolkit Of Translating Ribosome Affinity Purification, Joseph D. Dougherty

Open Access Publications

No abstract provided.

Osteoarthritis: Toward A Comprehensive Understanding Of Pathological Mechanism, Di Chen, Jie Shen, Weiwei Zhao, Tingyu Wang, Lin Han, John L. Hamilton, Hee-Jeong Im

Open Access Publications

No abstract provided.

Sparse Feature Selection For Classification And Prediction Of Metastasis In Endometrial Cancer, Mehmet Eren Ahsen, Todd P. Boren, Nitin K. Singh, Burook Misganaw, David G. Mutch, Kathleen N. Moore, Floor J. Backes, Carolyn K. Mccourt, Jayanthi S. Lea, David S. Miller, Michael A. White, Mathukumalli Vidyasagar

Open Access Publications

No abstract provided.

Listeria Monocytogenes Induces An Interferon-Enhanced Activation Of The Integrated Stress Response That Is Detrimental For Resolution Of Infection In Mice, Carolina Valderrama, Amy Clark, Fumihiko Urano, Emil R. Unanue, Javier A. Carrero

Open Access Publications

No abstract provided.

Multiplex Shrna Screening Of Germ Cell Development By In Vivo Transfection Of Mouse Testis, Nicholas R.Y. Ho, Abul R. Usmani, Yan Yin, Liang Ma, Donald F. Conrad

Open Access Publications

No abstract provided.

Anti-Angiogenic Nanotherapy Inhibits Airway Remodeling And Hyper-Responsiveness Of Dust Mite Triggered Asthma In The Brown Norway Rat, Gregory M. Lanza, John Jenkins, Anne H. Schmieder, Aigul Moldobaeva, Grace Cui, Huiying Zhang, Xiaoxia Yang, Qiong Zhong, Jochen Keupp, Ismail Sergin, Krishna S. Paranandi, Lindsey Eldridge, John S. Allen, Todd Williams, Michael J. Scott, Babak Razani, Elizabeth M. Wagner

Open Access Publications

No abstract provided.

Defining The 5΄ And 3΄ Landscape Of The Drosophila Transcriptome With Exo-Seq And Rnaseh-Seq, Shaked Afik, Osnat Bartok, Maxim N. Artyomov, Alexander A. Shishkin, Sabah Kadri, Mor Hanan, Xiaopeng Zhu, Manuel Garber, Sebastian Kadener

Open Access Publications

No abstract provided.

The Nuclear Receptor Hizr-1 Uses Zinc As A Ligand To Mediate Homeostasis In Response To High Zinc, Kurt Warnhoff, Hyun C. Roh, Zuzana Kocsisova, Chieh-Hsiang Tan, Andrew Morrison, Damari Croswell, Daniel L. Schneider, Kerry Kornfeld

Open Access Publications

No abstract provided.

Comparison Of Acoustic Recorders And Field Observers For Monitoring Tundra Bird Communities, Skyler T. Vold, Colleen M. Handel, Lance B. Mcnew

United States Geological Survey: Staff Publications

Acoustic recorders can be useful for studying bird populations but their efficiency and accuracy should be assessed in pertinent ecological settings before use. We investigated the utility of an acoustic recorder formonitoring abundance of tundra-breeding birds relative to point-count surveys in northwestern Alaska, USA, during 2014. Our objectives were to 1) compare numbers of birds and species detected by a field observer with those detected simultaneously by an acoustic recorder; 2) evaluate how detection probabilities for the observer and acoustic recorder varied with distance of birds from the survey point; and 3) evaluate whether avian guild-specific detection rates differed between …

The Aerosphere As A Network Connector Of Organisms And Their Diseases, Jeremy D. Ross, Eli S. Bridge, Diann J. Prosser, John Y. Takekawa

United States Geological Survey: Staff Publications

Aeroecological processes, especially powered flight of animals, can rapidly connect biological communities across the globe. This can have profound consequences for evolutionary diversification, energy and nutrient transfers, and the spread of infectious diseases. The latter is of particular consequence for human populations, since migratory birds are known to host diseases which have a history of transmission into domestic poultry or even jumping to human hosts. In this chapter, we present a scenario under which a highly pathogenic avian influenza (HPAI) strain enters North America from East Asia via postmolting waterfowl migration. We use an agent-based model (ABM) to simulate the …

Detoxification Of Mitochondrial Oxidants And Apoptotic Signaling Are Facilitated By Thioredoxin-2 And Peroxiredoxin-3 During Hyperoxic Injury, Benjamin J. Forred, Darwin R. Daugaard, Brianna K. Titus, Ryan R. Wood, Miranda J. Floen, Michelle L. Booze, Peter F. Vitiello

School of Veterinary and Biomedical Sciences: Faculty Publications

Mitochondria play a fundamental role in the regulation of cell death during accumulation of oxidants. High concentrations of atmospheric oxygen (hyperoxia), used clinically to treat tissue hypoxia in premature newborns, is known to elicit oxidative stress and mitochondrial injury to pulmonary epithelial cells. A consequence of oxidative stress in mitochondria is the accumulation of peroxides which are detoxified by the dedicated mitochondrial thioredoxin system. This system is comprised of the oxidoreductase activities of peroxiredoxin-3 (Prx3), thioredoxin-2 (Trx2), and thioredoxin reductase-2 (TrxR2). The goal of this study was to understand the role of the mitochondrial thioredoxin system and mitochondrial injuries during …

Maternal Inflammation At Mid-Gestation In Pregnant Rats Impairs Fetal Muscle Growth And Development At Term, C. N. Cadaret, K. A. Beede, E. M. Merrick, T. L. Barnes, J. D. Loy, D. T. Yates

School of Veterinary and Biomedical Sciences: Faculty Publications

Intrauterine growth restriction (IUGR) is a leading cause of perinatal morbidity and mortality. Low birth weight resulting from preterm birth and/or IUGR is an underlying factor in 60–80% of perinatal death worldwide, and is particularly common in developing countries (UNICEF, 2008). Furthermore, studies have linked IUGR and the associated fetal malnutrition to increased incidence of metabolic syndrome in adult life (Barker et al., 1993; Godfrey and Barker, 2000). The “thrifty phenotype hypothesis” developed by David Barker (Hales et al., 1991) states that IUGR-associated fetal malnutrition forces the fetus to spare nutrients by altering tissue-specific metabolism in order to survive. In …

A Parapoxviral Virion Protein Targets The Retinoblastoma Protein To Inhibit Nf-Κb Signaling, Ponnuraj Nagendraprabhu, Sushil Khatiwada, Sabal Chaulagain, Gustavo A. Delhon, Daniel L. Rock

School of Veterinary and Biomedical Sciences: Faculty Publications

Poxviruses have evolved multiple strategies to subvert signaling by Nuclear Factor κB (NF-κB), a crucial regulator of host innate immune responses. Here, we describe an orf virus (ORFV) virion-associated protein, ORFV119, which inhibits NF-κB signaling very early in infection (≤30 min post infection). ORFV119 NF-κB inhibitory activity was found unimpaired upon translation inhibition, suggesting that virion ORFV119 alone is responsible for early interference in signaling. A C-terminal LxCxE motif in ORFV119 enabled the protein to interact with the retinoblastoma protein (pRb) a multifunctional protein best known for its tumor suppressor activity. Notably, experiments using a recombinant virus containing an ORFV119 …

Characterization Of A New Chlorovirus Type With Permissive And Non-Permissive Features On Phylogenetically Related Algal Strains, Cristian F. Quispe, Ahmed Esmael, Olivia Sonderman, Michelle Mcquinn, Irina Agarkova, Mohammed Battah, Garry A. Duncan, David D. Dunigan, Timothy P. L. Smith, Cristina Decastro, Immacolata Speciale, Fangrui Ma, James L. Van Etten

Nebraska Center for Virology: Faculty Publications

A previous report indicated that prototype chlorovirus PBCV-1 replicated in two Chlorella variabilis algal strains, NC64A and Syngen 2-3, that are ex-endosymbionts isolated from the protozoan Paramecium bursaria. Surprisingly, plaque-forming viruses on Syngen 2-3 lawns were often higher than on NC64A lawns from indigenous water samples. These differences led to the discovery of viruses that exclusively replicate in Syngen 2-3 cells, named Only Syngen (OSy) viruses. OSy-NE5, the prototype virus for the proposed new species, had a linear dsDNA genome of 327 kb with 44-nucleotide-long, incompletely base-paired, covalently closed hairpin ends. Each hairpin structure was followed by an identical …

Salsalate Treatment Following Traumatic Brain Injury Reduces Inflammation And Promotes A Neuroprotective And Neurogenic Transcriptional Response With Concomitant Functional Recovery, Mouna Lagraoui, Gauthaman Sukumar, Joseph R. Latoche, Sean K. Maynard, Clifton L. Dalgard, Brian C. Schaefer

Uniformed Services University of the Health Sciences

Neuroinflammation plays a critical role in the pathogenesis of traumatic brain injury (TBI). TBI induces rapid activation of astrocytes and microglia, infiltration of peripheral leukocytes, and secretion of inflammatory cytokines. In the context of modest or severe TBI, such inflammation contributes to tissue destruction and permanent brain damage. However, it is clear that the inflammatory response is also necessary to promote post-injury healing. To date, anti-inflammatory therapies, including the broad class of non-steroidal anti-inflammatory drugs (NSAIDs), have met with little success in treatment of TBI, perhaps because these drugs have inhibited both the tissue-damaging and repair-promoting aspects of the inflammatory …

High Pesticide Exposure Events And Dna Methylation Among Pesticide Applicators In The Agricultural Health Study, Jennifer A. Rusiecki, Laura E. Beane Freeman, Matthew R. Bonner, Melannie Alexander, Ligong Chen, Gabriella Andreotti, Kathryn H. Barry, Lee E. Moore, Hyang-Min Byun, Freya Kamel, Michael Alavanja, Jane A. Hoppin, Andrea Baccarelli

Uniformed Services University of the Health Sciences

Pesticide exposure has been associated with acute and chronic adverse health effects. DNA methylation (DNAm) may mediate these effects. We evaluated the association between experiencing unusually high pesticide exposure events (HPEEs) and DNAm among pesticide applicators in the Agricultural Health Study (AHS), a prospective study of applicators from Iowa and North Carolina. DNA was extracted from whole blood from male AHS pesticide applicators (n5695). Questionnaire data were used to ascertain the occurrence of HPEEs over the participant’s lifetime. Pyrosequencing was used to quantify DNAm in CDH1, GSTp1, and MGMT promoters, and in the repetitive element, LINE-1. Linear and robust regression …

Prevention Of Insulin Resistance In Adolescents At Risk For Type 2 Diabetes With Depressive Symptoms: 1-Year Follow-Up Of A Randomized Trial, Lauren B. Shomaker, Nichole R. Kelly, Rachel M. Radin, Omni L. Cassidy, Lisa M. Shank, Sheila M. Brady, Andrew P. Demidowich, Cara H. Olsen, Kong Y. Chen, Eric Stice, Marian Tanofsky-Kraff, Jack A. Yanovski

Uniformed Services University of the Health Sciences

Background: Depression is associated with poor insulin sensitivity. We evaluated the long-term effects of a cognitive behavioral therapy (CBT) program for prevention of depression on insulin sensitivity in adolescents at risk for type 2 diabetes (T2D) with depressive symptoms.

Methods: One-hundred nineteen adolescent females with overweight/obesity,T2Dfamily history, and mild-to-moderate depressive symptoms were randomized to a 6-week CBT group (n = 61) or 6-week health education (HE) control group (n = 58). At baseline, posttreatment, and 1 year, depressive symptoms were assessed, and whole body insulin sensitivity (WBISI) was estimated from oral glucose tolerance tests.Dual energy X-ray absorptiometry assessed fat mass …

Evaluating The Impact Of Ambient Benzene Vapor Concentrations On Product Water From Condensation Water From Air Technology, Katherine M. Kinder, Christopher A. Gellasch, James S. Dusenbury, Thomas C. Timmes, Thomasm. Hughes

Uniformed Services University of the Health Sciences

Globally, drinking water resources are diminishing in both quantity and quality. This situation has renewed interest in Condensation Water From Air (CWFA) technology,which utilizes water vapor in the air to produce water for both potable and non-potable purposes. However, there are currently insufficient data available to determine the relationship between air contaminants and the rate at which they are transferred from the air into CWFA untreated product water. This study implemented a novel experimental method utilizing an environmental test chamber to evaluate how air quality and temperature affects CWFA untreated product water quality in order to collect data that will …

Intraspecies Comparative Genomics Of Three Strains Of Orientia Tsutsugamushi With Different Antibiotic Sensitivity, Hsiao-Mei Liao, Chien-Chung Chao, Haiyan Lei, Bingjie Li, Shien Tsai, Guo-Chiuan Hung, Wei-Mei Ching, Shyh-Ching Lo

Uniformed Services University of the Health Sciences

We recently reported the genome of Orientia tsutsugamushi (OT) strain Karp (GenBank Accession #: NZ_LYMA00000000.2, https://www.ncbi.nlm.nih.gov/nuccore/NZ_LYMA00000000.2) with N2 Mb in size through clone-based sequencing and high throughput genomic shotgun sequencing (HTS). The genomes of OT strains AFSC4 and AFSC7 were similarly sequenced by HTS Since strains AFSC4 (GenBank Accession #: NZ_LYMT00000000.1, https://www.ncbi.nlm.nih.gov/nuccore/1035784408) and AFSC7 (GenBank Accession #: NZ_LYMB00000000.1, https://www.ncbi.nlm.nih.gov/nuccore/1035854767) were more resistant to antibiotics than strain Karp, we conducted comparative analysis of the three draft genomes annotated by RAST server aimed to identify possible genetic bases of difference in microbial antibiotic sensitivity. Intraspecies comparative genomics analysis of the three OT …

Clonal Distribution And Associated Characteristics Of Escherichia Coli Clinical And Surveillance Isolates From A Military Medical Center, Amee R. Manges, Katrin Mende, Clinton K. Murray, Brian D. Johnson, Evgeni V. Sokurenko, Veronika Tchesnokova, James R. Johnson

Uniformed Services University of the Health Sciences

Antimicrobial-resistant Escherichia coli are a concern for military health services. We studied 100 extended-spectrum beta-lactamase (ESBL)-producing and non-producing E. coli clinical and surveillance isolates from military personnel and civilians at Brooke Army Medical Center (2007–2011). Major E. coli lineages, most prominently ST10 (24%), ST131 (16%), and ST648 (8%), were distributed much as reported for other North American populations. ST131, represented mainly by its resistance-associated ST131-H30 clonal subset,was uniquely associated with a clinical origin, regardless of ESBL status. Thus, clonal background predicted resistance phenotype and clinical versus surveillance origin, and these findings could assist military clinicians and epidemiologists.

Structural Studies Demonstrating A Bacteriophage-Like Replication Cycle Of The Eukaryote-Infecting Paramecium Bursaria Chlorella Virus-1, Elad Milrot, Eyal Shimoni, Katya Rechav, Tamar Unger, James L. Van Etten, Abraham Minsky

James Van Etten Publications

A fundamental stage in viral infection is the internalization of viral genomes in host cells. Although extensively studied, the mechanisms and factors responsible for the genome internalization process remain poorly understood. Here we report our observations, derived from diverse imaging methods on genome internalization of the large dsDNA Paramecium bursaria chlorella virus-1 (PBCV-1). Our studies reveal that early infection stages of this eukaryotic- infecting virus occurs by a bacteriophage-like pathway, whereby PBCV-1 generates a hole in the host cell wall and ejects its dsDNA genome in a linear, base-pair-by-base-pair process, through a membrane tunnel generated by the fusion of the …

2017 Update On Pain Management In Patients With Chronic Kidney Disease, Phuong Chi Pham, Kathy Khaing, Theodore M. Sievers, Phuong Mai Pham, Jeffrey M. Miller, Son V. Pham, Phuong Anh Pham, Phuong Thu Pham

United States Department of Veterans Affairs: Publications

The prevalence of pain has been reported to be >60–70% among patients with advanced and end-stage kidney disease. Although the underlying etiologies of pain may vary, pain per se has been linked to lower quality of life and depression. The latter is of great concern given its known association with reduced survival among patients with end-stage kidney disease.We herein discuss and update the management of pain in patients with chronic kidney disease with and without requirement for renal replacement therapy with the focus on optimizing pain control while minimizing therapy-induced complications.

University Of Nebraska- Lincoln: Fact 2016-2017

University of Nebraska-Lincoln Administration: Reports, Papers, Publications, and Presentations

Table of Contents

Front Cover......................................................................................... 1

Introduction ................................................................................... 2

Table of Contents ............................................................................................ 3

General Information

University of Nebraska-Lincoln Core Values (LEADERS) .................................... 6

University of Nebraska-Lincoln Mission ....................................................... 7

The Missions of the University of Nebraska-Lincoln ......................................... 7

Teaching.................................................................................................... 8

Research............................................................................................ 8

Service ............................................................................................ 9

Institutional and Professional Accreditations .....................................................10

University of Nebraska-Lincoln Administrative Organization Chart .....................14

Student Credit Hours (SCH)

Total SCH: Fall Semester Since 1979 ........................................................15

Total SCH: Spring Semester Since 1993 .....................................................16

SCH by College and Course Level, Fall and Spring Semester, 5 Year Trend ....17

SCH by College and Course Level, Fall Semester, 5 Year …

Compensatory Response Of Fathead Minnow Larvae Following A Pulsed In-Situ Exposure To A Seasonal Agricultural Runoff Event, Jonathan M. Ali, John Sangster, Daniel D. Snow, Shannon L. Bartelt-Hunt, Alan S. Kolok

Nebraska Water Center: Faculty Publications

Agriculturally-dominated waterways such as those found throughout the Midwestern United States often experience seasonal pulses of agrichemical contaminants which pose a potential hazard to aquatic organisms at varying life stages. The objective of this study was to characterize the developmental plasticity of fathead minnow larvae in a natural environment subject to a seasonal episodic perturbation in the form of a complex mixture of agricultural stressors. Fathead minnow larvae were maintained at the Elkhorn River Research Station for a 28-d in situ exposure to an agrichemical pulse event.Minnow larvae were sampled after 14 and 28 days to characterize developmental plasticity through …

Increased Insertion Number Leads To Increased Sperm Transfer And Fertilization Success In A Nursery Web Spider, Alissa G. Anderson, Eileen Hebets

Eileen Hebets Publications

Across animals, a male's fitness is largely dictated by his ability to fertilize eggs; and there exists a plethora of male adaptations associated with increasing fertilization success. In the nursery web spider, Pisaurina mira, males restrain females prior to and during copulation by wrapping them with silk. Previous research demonstrates that copulatory silk wrapping reduces a male's chance of being sexually cannibalized and increases the number of sperm transfer opportunities (termed insertions) that a male can achieve within a mating. While avoiding cannibalism provides an obvious survival benefit to males, the impact of insertion number on male fitness remains …

Formulations Of Primer, Acrylic Paint And Pastel In Traditional Thai Style Colour, Nawarat Kaew-On

Chulalongkorn University Theses and Dissertations (Chula ETD)

This research aimed to improve Thai traditional primer, used for mural painting, with antibacterial property, to formulate novel primers having antibacterial behavior, scrub resistance and washability as well as to formulate paints according to Thai color dictionary. Both primers and paints were aimed to use in mural painting. We divided the research into 3 parts: 1) improved traditional primer and formulated novel primer with antibacterial, washable and scrub resistance properties; 2) formulated acrylic paints and pastels according to Thai color dictionary and 3) investigated the wavelengths used for distinguishing the change of paint caused by light, moist and heat using …

Doxorubicin-Conjugated Dexamethasone Induced Mcf-7 Apoptosis Without Entering The Nucleus And Able To Overcome Mdr-1-Induced Resistance, Kamontip Chaikomon

Chulalongkorn University Theses and Dissertations (Chula ETD)

Doxorubicin is a common chemotherapeutic drug that generally uses in many solid and hematologic malignancies. The main mechanism of doxorubicin is inhibition of topoisomerase II that occurs in the nucleus. Currently, doxorubicin resistant cancer cells which have decreased accumulation of intracellular doxorubicin resulting from an efflux pump, P-glycoprotein encoded by multidrug resistant gene. Several studies reported many different ways to overcome doxorubicin resistance. In this study, we conjugated 3'amino group of doxorubicin to dexamethasone molecule. Despite of lower cytotoxic activity in MCF-7 cells, the conjugated product, DexDOX, exerted its actions in the different fashion to doxorubicin. DexDOX rapidly induced MCF-7 …

Development Of Innovative High-Energy Neutron And Gamma-Ray Shielding Material Based On High Density Cement And Crumb Rubber, Pawitra Aim-O

Chulalongkorn University Theses and Dissertations (Chula ETD)

The incorporation of carbon and hydrogen-rich material into radiation shielding material can improve its shielding performance. High-density cement mixed with crumb rubber was studied to be a gamma ray and neutron shielding material. Shielding materials were designed using high-density cement mixed with 5 - 25 vol% of crumb rubber. Monte Carlo N-Particle code was employed to simulate radiation shielding performances of the composed materials. The utilized neutron and photon sources were Am-241/Be and Co-60, respectively. These shielding designs were fabricated and experimentally evaluated on the radiation shielding properties. The cracking mechanism of high-density cement mixed with lead (HDCRL) was also …