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Plasma Enhanced Chemical Vapor Deposition Coating Amorphous Silica Based Implants Enhancing Angiogenesis And Mitigating Toxic Oxidative Stress In Critical Size Bone Defects, Felipe Alves Monte

Bioengineering Dissertations - Archive

The advances in bone tissue engineering search for a biomaterial that could efficiently and gradually replace the bone while the new tissue grows through the implanted structure. However, due to limitations mainly related to mechanical properties and challenges, such as the balance between tissue regeneration and material degradation, the coating of well-known inert materials still has been used in orthopedics and dental clinical practices. Bone defects are commonly related to deleterious oxidative stress induced by low oxygen levels and inflammation. Toxic oxidative stress can impair damaged tissue healing due to inhibiting new blood vessels and new bone formation. The interaction …

Erosion And Accretion On A Mudflat: The Importance Of Very Shallow-Water Effects, Benwei Shi, James R. Cooper, Paula D. Pratolongo, Shu Gao, T. J. Bouma, Gaocong Li, Chunyan Li, S. L. Yang, Ya Ping Wang

Faculty Publications

Understanding erosion and accretion dynamics during an entire tidal cycle is important for assessing their impacts on the habitats of biological communities and the long-term morphological evolution of intertidal mudflats. However, previous studies often omitted erosion and accretion during very shallow-water stages (VSWS, water depths < 0.20 m). It is during these VSWS that bottom friction becomes relatively strong and thus erosion and accretion dynamics are likely to differ from those during deeper flows. In this study, we examine the contribution of very shallow-water effects to erosion and accretion of the entire tidal cycle, based on measured and modeled time-series of bed-level changes. Our field experiments revealed that the VSWS accounted for only 11% of the duration of the entire tidal cycle, but erosion and accretion during these stages accounted for 35% of the bed-level changes of the entire tidal cycle. Predicted cumulative bed-level changes agree much better with measured results when the entire tidal cycle is modeled than when only the conditions at water depths of >0.2 m (i.e., probe submerged) are considered. These findings suggest that the magnitude of bed-level changes during VSWS should not be neglected when modeling morphodynamic processes. Our results are useful in understanding the mechanisms of micro-topography formation and destruction that often occur at VSWS, and also improve our understanding and modeling ability of coastal morphological …

The Route To Water Security For Texas: The 2015–2016 Texas Water Roadmap Forums, Rudolph A. Rosen, Rabi Mohtar, Luis A. Cifuentes, Stephen Frayser, Gwendolyn Hustvedt, Wesley Patrick, Chara Ragland, Susan V. Roberts, Jorge Vanegas, Cindy Wall, James Wall

Water Resources Science and Technology Faculty Publications (Archived)

Three forums were held between February 2015 and November 2016, bringing together Texas water experts from business, industry, government, academia, research, and the investment community in impartially facilitated sessions to deter-mine ways to secure Texas’ water future through accelerating growth of infrastructure, technologies, research, education, and sustainable use. Consensus emerged after the first forum that Texas is approaching a water crisis reflecting matters of supply, allocation, and quality that demands immediate action to ensure water security and equitable access to this vital resource. Participant focus rested on new technology acceleration and investment, workforce education, research underway and desired by segments …

Comparing Methods Of Targeting Obesity Interventions In Populations: An Agent-Based Simulation, Rahmatollah Beheshti, Mehdi Jalalpour, Thomas A. Glass

Civil and Environmental Engineering Faculty Publications

Social networks as well as neighborhood environments have been shown to effect obesity-related behaviors including energy intake and physical activity. Accordingly, harnessing social networks to improve targeting of obesity interventions may be promising to the extent this leads to social multiplier effects and wider diffusion of intervention impact on populations. However, the literature evaluating network-based interventions has been inconsistent. Computational methods like agent-based models (ABM) provide researchers with tools to experiment in a simulated environment. We develop an ABM to compare conventional targeting methods (random selection, based on individual obesity risk, and vulnerable areas) with network-based targeting methods. We adapt …

2017-2018 Winter - University School Alumni Newsletter, University School, East Tennessee State University

University School Newsletters

No abstract provided.

Full Issue

Journal of Prison Education and Reentry (2014-2023)

No abstract provided.

High Annual Survival In Infected Wildlife Populations May Veil A Persistent Extinction Risk From Disease, Brooke Maslo, Oliver C. Stringham, Amanda J. Bevan

KIP Articles

Host response to emerging pathogens is variable, causing uncertainty about population‐level impacts and challenging effective disease management. White‐nose syndrome (WNS) has caused catastrophic declines in some bat species, while others appear less impacted. Developing predictive models based on observed survival patterns can generate testable hypotheses about mechanisms driving population dynamics and contribute to the development of targeted approaches to disease management. We conducted a mark–recapture study of federally endangered Indiana bats (Myotis sodalis) during 2011–2016. Annual survival decreased from 0.78 (95% CI: 0.59, 0.89) and 0.79 (95% CI: 0.70, 0.86) for females and males, respectively, in 2011 to 0.74 (95% …

Insights Into The Ancestral Organisation Of The Mammalian Mhc Class Ii Region From The Genome Of The Pteropid Bat, Pteropus Alecto, Justin H. J. Ng, Mary Tachedjian, Lin-Fa Wang, Michelle L. Baker

KIP Articles

No abstract provided.

Representation Of Deaf Characters And Culture In Young Adult Fiction, Kimberly Gangwish

Educational Leadership Theses, Dissertations, and Student Creative Activity

Adolescence is a time when teenagers begin to develop a sense of self and the cultures they belong to. Literature is a societal artifact that can inform and influence the development of cultural identity. Deaf culture is unique because a person is not born into the culture, but can choose to become a member of the culture. This cultural group is underrepresented in young adult literature. This underrepresentation places more importance on accurate representations of Deaf culture since young adult fiction may be the only exposure to Deaf culture that both hearing and deaf teenagers may have.

This study analyzed …

Human Metapneumovirus Induces Formation Of Inclusion Bodies For Efficient Genome Replication And Transcription, Nicolás P. Cifuentes-Muñoz, Jean Branttie, Kerri Beth Slaughter, Rebecca Ellis Dutch

Molecular and Cellular Biochemistry Faculty Publications

Human metapneumovirus (HMPV) causes significant upper and lower respiratory disease in all age groups worldwide. The virus possesses a negative-sense single-stranded RNA genome of approximately 13.3 kb encapsidated by multiple copies of the nucleoprotein (N), giving rise to helical nucleocapsids. In addition, copies of the phosphoprotein (P) and the large RNA polymerase (L) decorate the viral nucleocapsids. After viral attachment, endocytosis, and fusion mediated by the viral glycoproteins, HMPV nucleocapsids are released into the cell cytoplasm. To visualize the subsequent steps of genome transcription and replication, a fluorescence in situ hybridization (FISH) protocol was established to detect different viral RNA …

Depression In Stroke Patients; A Cross Sectional Study Highlighting The Association Of Stroke On Age And Gender Basis., Saira Abbas, Naila Naeem Shahbaz, Muhammad Amir Umer, Sumera Rafat Umer, Asfia Irfan, Adnan Anwar

Pakistan Journal of Neurological Sciences (PJNS)

Stroke is the second leading cause of death. Depressive disorder is characterized by period of sad mood and anhedonia occurring for two consecutive weeks. Among stroke survivors, the consequence of physical and psychological changes can be devastating. One of those psychological changes is post-stroke depression (PSD). Stroke itself has debilitating morbidity and superimposed PSD further decreases the quality of life of patients and impairs recovery. This study leads us to know the magnitude of post stroke depression.

A Rare Case Of Becker Disease In A 7 Year Old Boy, Prem Chand, Rozmeen Badruddin Husein, Fazal M. Arain

Pakistan Journal of Neurological Sciences (PJNS)

Becker Disease is an autosomal recessive version of the rare congenital disorder called Myotonia Congenita. Due to the rarity of Becker Disease, the genetic and pathological basis of this disease have not been studied well and possible diagnostic methods and techniques are yet to be explored. The existing method of diagnosis of such a case is predominantly dependent on the clinical examination. More work and studies need to be done on the diagnostic aspect of this disease to discover newer diagnostic methods for this disease, which are more reliable and specific. In order to develop better diagnostic methods for the …

Epidemiological Data Of Neurological Disorders In Pakistan And Neighboring Countries: A Review, Ghulam Hussain, Azhar Rasul, Haseeb Anwar, Muhammad Umar Sohail, Syed Kashif Shahid Kamran, Shahid Mahmood Baig, Asghar Shabbir, Javed Iqbal

Pakistan Journal of Neurological Sciences (PJNS)

Neurological disorders are the impairments of nervous system and are an important and growing cause of morbidity, mortality, and disability. In addition to health costs, those suffering from these conditions are also frequently victimized of stigmatization and discrimination. Stigmatization further minimizes the patients' access to treatment and social activities. These disorders, therefore, require special attention particularly in developing countries where unfortunately, the burden of these disorders remains largely unrecognized. Moreover, the burden imposed by such chronic neurological conditions in general can be expected to be particularly devastating in poor populations. These conditions are emerging as severe public health concerns in …

Deconstructing The Ivorian Vestimentary Traditions: New Fashion, Contemporary Beauty And New Identity In Marguerite Abouet And Clément Oubrerie’S Aya De Yopougon, Richard Oko Ajah, Letitia Egege

Wagadu: A Journal of Transnational Women's & Gender Studies

This paper adopts an eclectic framework of semiotic, postmodern and postcolonial theories to interpret the representations of dressing as an aesthetic activity in Aya de Yopougon 1-3 and to investigate how Yopougon dwellers use their fashion sense to establish both a group identity and a form of everyday resistance. Characters’ bodies are remade, through dressing, to contain emotive qualities and to play symbolic functions with their everyday choice of dress. Their sartorial obsession is supported by psychic inferiority and pop culture; all characters engage in “disciplinary practices” for the clothing culture of their bodies that are ornamented surfaces for display.

Effects Of Edible Coating On The Sensory And Physiochemical Properties Of Roasted Coffee Beans Stored At Ambient Temperature, Tuan Quoc Le

Manipal Journal of Science and Technology

Roasted coffee beans during industrialized processing are difficult to retain volatile compounds and flavour. Several methods, especially edible coating have been studied and used widely in the world, while applications for Vietnamese coffee beans are still limited. In this study, the effects of edible coating with xanthan gum at different concentrations, 0.5%, 1%, and 1.5% (w/v) on sensory and physiochemical properties of two major types of coffee, Robusta and Arabica were investigated. The results presented the positive effects on the sensory and physiochemical properties of both two types of coffee during 30-day-storage at ambient temperature. The hardness, pH value, moisture …

Ancient Amazonian Populations Left Lasting Impacts On Forest Structure, Michael W. Palace, C. N. H. Mcmichael, Bobby H. Braswell, S. C. Hagen, M. B. Bush, E. Neves, E. Tamanaha, Christina Herrick, Stephen E. Frolking

All Materials

Amazonia contains a vast expanse of contiguous tropical forest and is influential in global carbon and hydrological cycles. Whether ancient Amazonia was highly disturbed or modestly impacted, and how ancient disturbances have shaped current forest ecosystem processes, is still under debate. Amazonian Dark Earths (ADEs), which are anthropic soil types with enriched nutrient levels, are one of the primary lines of evidence for ancient human presence and landscape modifications in settings that mostly lack stone structures and which are today covered by vegetation. We assessed the potential of using moderate spatial resolution optical satellite imagery to predict ADEs across the …

Family History Of Kelsie Mart, Kelsie Mart

Your Family in History: HIST 550/700

The purpose of this research paper was to use information given to me by relatives, as well as information that was found on familysearch, to get a family history written with information regarding my family and their history. Using the familysearch source, I was able to find important dates, census reports from many years ago, different documents and family stories, marriage and death records, and much more valuable information that helped to create this PDF. This paper has information over my family that was not known to me before and using the sources and hearing the stories I did, I …

Blueprint For Survival: A New Paradigm For International Environmental Emergencies, Claire Wright

Fordham Environmental Law Review

No abstract provided.

Enhancer Interactions Between Pese And Sog At The Slp1 Promoter Allow Transcription Initiation In The Presence Of Runt And Ftz, Jennifer Taylor Shapp

Stony Brook Theses and Dissertations Collection, 2006-2020 (closed to submissions)

The slp1 gene has two upstream enhancer cis-regulatory modules that regulate transcription by non-additive interactions, the Proximal Early Stripe Element (PESE) and the Distal Early Stripe Element (DESE). These two enhancers are regulated by pair-rule transcription factors Even-skipped (Eve), Odd-Pared (Opa), Runt, and Fushi tarazu (Ftz). Both enhancers are repressed by Runt and Ftz, but only PESE is repressed by Eve. PESE and DESE are regulated along the antero-posterior axis of the 3hr Drosophila embryo while the short-gastrulation (sog) enhancer responds to regulation on the dorso-ventral axis. By combining the sog enhancer in a reporter gene construct with the PESE …

Examination Of The Role Of The Microenvironment To Cell Invasive Behavior During Uterine-Vulval Attachment In Caenorhabditis Elegans And Oscheius Tipulae, Simeiyun Liu

Stony Brook Theses and Dissertations Collection, 2006-2020 (closed to submissions)

Though cell invasion behavior is critical for developmental processes and tissue integrity, the molecular mechanisms that integrate both the extracellular signals with cell-autonomous regulators remains poorly understood. To investigate the microenvironmental signals contributing to anchor cell (AC) invasion in C. elegans, I focused here on two extrinsic signals: the netrin signaling pathway and the unknown vulval chemical cue. I examined the interaction of these cues in C. elegans and determined their role in regulating AC invasion in a distantly related nematode species, O. tipulae. By assessing the influence of loss of the chemical cue to netrin signaling and invasive protrusion …

De Novo Design Targeting Hivgp41 [Title Given At Proquest Is De Novo Design Targeting Hiv Gp-41], Brian Christopher Fochtman

Stony Brook Theses and Dissertations Collection, 2006-2020 (closed to submissions)

In this dissertation, we discuss the implementation and evaluation of several improvements to the DOCK6 codebase relevant to the development of small molecule HIVgp41 inhibitors. In Chapter1, we give a brief introduction for the dissertation. This includes a description of the mechanism of HIV fusion, and a summary of existing FDA approved HIV inhibitors along with a history of not yet approved gp41 fusion inhibitors. Further, this chapter introduces the computational techniques of docking and molecular dynamics which are utilized throughout this work. In Chapter 2, we describe the development and validation of a de novo design protocol for DOCK6. …

The Roles Ntl, Wnt, And Bmp Play On P21 Expression Levels In Development, Mian Chen

Stony Brook Theses and Dissertations Collection, 2006-2020 (closed to submissions)

p21 is an important CDK inhibitor (CKI) which is known to inhibit cell cycle progression by down-regulating CDK2 levels. For that reason, p21 is an excellent indicator to study cell cycle regulation. By the end of mitosis, cells with low levels of p21 proceed to S phase, while cells with high levels of p21 arrest in the G1/G0 phase of the cell cycle. In previous studies, the embryonic transcription factor Brachyury is shown to be crucial to posterior body development. However, it is unknown whether brachyury also plays a role in cell cycle regulation, which may contribute to the phenotype …

Integrative Image And Spatial Analytics For Three-Dimensional Digital Pathology, Yanhui Liang

Stony Brook Theses and Dissertations Collection, 2006-2020 (closed to submissions)

3D digital pathology provides extreme scale quantitative data with high potential for basic research in a wide scope, and becomes an emerging technology promising to support computer aided diagnosis. Quantitative analyses of 3D pathology images involve both deriving 3D micro-anatomic objects and their features, and exploring spatial relationships among a massive number of biological objects. However, this is challenged by the overwhelming data scale and 3D pathology complexity. Our goal is to create a scalable and effective 3D digital pathology analytics framework for large-scale 3D pathology imaging data. The framework will provide novel methods on pathology image registration, segmentation, and …

Quantitative Foraging Ecology Of Terns At Great Gull Island, New York: Theory, Models, And Remote Sensing Of Collective Behavior In A Dynamic Habitat, Samuel Stetson Urmy

Stony Brook Theses and Dissertations Collection, 2006-2020 (closed to submissions)

Most seabirds forage for prey whose distributions are patchy and unpredictable, and their strategies for locating food have long interested ecologists. Recent studies using individual telemetry have yielded many insights, but our understanding is still limited: simultaneous measurements of prey and predator distributions are rare, and tracking tags can be deployed on only small numbers of animals at once. I addressed these challenges using a marine radar deployed at a breeding colony of common and roseate terns (Sterna hirundo and S. dougallii) on Great Gull Island, New York, in 2014 and 2015. I calibrated the radar, enabling measurements of tern …

Natural And Synthetic Biohydrogels Design, Characterization, Network Structure Imaging And Modeling, Clement Marmorat

Stony Brook Theses and Dissertations Collection, 2006-2020 (closed to submissions)

Biocompatible hydrogels can be derived from materials that are naturally obtained, such as proteins or polysaccharides, or synthetic, such as poloxamers. In order to be classified as biocompatible, these water-swollen networks can not trigger a toxic response once introduced into a biological or physiological environment and, therefore, must be immunoneutral. Hyaluronic acid hydrogels can be great candidates for tissue engineering applications as long as the cross-linking chemistry and process does not affect the biocompatibility of the natural protein matrix. Thermoreversible hydrogels have the advantage of undergoing a sol/gel phase transition at specific temperatures. Thus, they are excellent candidates for biomedical …

Effects Of Sterols Upon Clathrin-Mediated Or -Independent Endocytosis And Uptake Of Yersinia Pseudotuberculosis, Ji Hyun Kim

Stony Brook Theses and Dissertations Collection, 2006-2020 (closed to submissions)

Ordered membrane domains rich in sphingolipids and sterols (lipid rafts) are thought to be important in many biological processes. However, it is difficult to distinguish domain-dependent biological functions from ones having a specific sterol dependence. Sterol substitution has the potential to help distinguish these cases. This study uses sterol substitution to probe effects of sterol properties upon two cellular functions; endocytosis and bacterial uptake into mammalian cells. Lipid rafts have been hypothesized to participate in endocytosis based on inhibition of endocytosis by removal of cholesterol. To investigate the role of the sterol in endocytosis, we replaced cholesterol in MDA-MB-231 cells …

Development Of Novel Therapeutic Strategies For Targeting Metastatic Cancer Cells And Overcoming Cancer Stem Cell Chemoresistance, Vincent Michael Alford

Stony Brook Theses and Dissertations Collection, 2006-2020 (closed to submissions)

90% of all cancer related patient mortalities are due to metastasis, the process by which cancer cells spread and seed secondary tumors at distant sites in the body. Currently, there are no effective therapies aimed at targeting metastasis due to the complex nature and multitude of pathways involved in this biological process. To address this important aspect of cancer biology, we sought to investigate key modulators of the extracellular matrix (ECM), as this is a critical determinant for a tumor cells capacity to escape and invade adjacent tissue structures. Matrix Metalloproteinases (MMPs) represent the most prominent family of proteinases associated …

Pik3ca-Stimulated Glycolytic & Aerobic Activity Promotes Kras-Driven Pancreatic Tumor Progression, Stephanie Rose Chapelliquen

Stony Brook Theses and Dissertations Collection, 2006-2020 (closed to submissions)

Pancreatic ductal adenocarcinoma is the 3rd leading cause of cancer-related death with a <5% five-year survival rate. We previously demonstrated that PI3K p110? (gene name Pik3ca) is required for oncogenic Kras to initiate pancreatic tumors in mice. To study the role of Pik3ca in pancreatic tumors after they are formed, we employed CRISPR/Cas9 to delete Pik3ca in cultured cancer cells derived from the Kras(G12D/+);Pdx1Cre (KC) pancreatic tumor mouse model. Silencing Pik3ca impaired cell proliferation and invasion, as well as decreased glycolysis and oxidative phosphorylation. Pharmacologic inhibition of p110? using BYL719 showed similar results. Orthotopic implantation of KC cell lines in the pancreas of C57BL/6 mice showed that Pik3ca deletion decreased tumor growth resulting in significantly increased median survival (parental 28 days vs. Pik3ca-null 94.5 days). Pik3ca-null KC cells exhibit decreased PGC1? expression and these cellular phenotypes were all reversed by PGC1? overexpression. To study the requirement of Pik3ca in a spontaneous pancreatic tumor mouse model, we produced mice with pancreata harboring the KrasG12D mutation that express a tetracycline-regulated Pik3ca transgene but lacking endogenous Pik3ca alleles. Turning off transgenic Pik3ca decreased PGC1? expression. In summary, our findings indicate that p110? supports invasive growth of pancreatic tumors by regulating the expression of PGC1?. | 100 pages

Regulation Of Fyn Phosphorylation By The Ptpn23 Tumor Suppressor Phosphatase In Breast Tumorigenesis, Siwei Zhang

Stony Brook Theses and Dissertations Collection, 2006-2020 (closed to submissions)

Disruption of the balanced modulation of reversible tyrosine phosphorylation has been implicated in the etiology of various human cancers. Breast cancer is the most prevalent invasive malignancy among women in the US. It features high heterogeneity, divisible into a variety of subtypes with different molecular profiles and clinical status. Protein Tyrosine Phosphatase Non-receptor 23 (PTPN23) gene resides on chromosomal region 3p21.3, which is hemizygously or homozygously lost in various cancers, including breast cancer of different subtypes. In a loss.of.function PTPome screen, our laboratory previously identified PTPN23 as a suppressor of cell motility and invasion in mammary epithelial and breast cancer …

The Intracellular Cell Biology Of Ionotropic Glutamate Receptors, Noele Doreen Certain

Stony Brook Theses and Dissertations Collection, 2006-2020 (closed to submissions)

AMPARs play an essential role in fast excitatory neurotransmission. Before native AMPARs can function at the synapse they must be processed intracellularly. These ion channels are synthesized and assembled into multimeric complexes, shuttled in a regulated fashion between intracellular compartments and are finally inserted into the membrane to carry out their function. Important binding partners known as auxiliary subunits associate with AMPARs at the synapse, but also during assembly and trafficking. The presence of auxiliary subunits in AMPAR complexes at the membrane has been demonstrated through electrophysiological studies, showing effects on the gating and pharmacology of AMPARs. The localization of …