Digital Commons Network^™

Pulmonary Microrna Profiles Identify Involvement Of Creb1 And Sec14l3 In Bronchial Epithelial Changes In Allergic Asthma., Sabine Bartel, Nikola Schulz, Francesca Alessandrini, Andrea C Schamberger, Philipp Pagel, Fabian J Theis, Katrin Milger, Elfriede Noessner, Stephen M Stick, Anthony Kicic, Oliver Eickelberg, Robert J Freishtat, Susanne Krauss-Etschmann

Medicine Faculty Publications

Asthma is highly prevalent, but current therapies cannot influence the chronic course of the disease. It is thus important to understand underlying early molecular events. In this study, we aimed to use microRNAs (miRNAs) - which are critical regulators of signaling cascades - to identify so far uncharacterized asthma pathogenesis pathways. Therefore, deregulation of miRNAs was assessed in whole lungs from mice with ovalbumin (OVA)-induced allergic airway inflammation (AAI). In silico predicted target genes were confirmed in reporter assays and in house-dust-mite (HDM) induced AAI and primary human bronchial epithelial cells (NHBE) cultured at the air-liquid interface. We identified and …

Molecular Detection Of Viruses In Kenyan Bats And Discovery Of Novel Astroviruses, Caliciviruses And Rotaviruses, Cecilia Waruhiu, Sheila Ommeh, Vincent Obanda

KIP Articles

This is the first country-wide surveillance of bat-borne viruses in Kenya spanning from 2012–2015 covering sites perceived to have medium to high level bat-human interaction. The objective of this surveillance study was to apply a non-invasive approach using fresh feces to detect viruses circulating within the diverse species of Kenyan bats. We screened for both DNA and RNA viruses; specifically, astroviruses (AstVs), adenoviruses (ADVs), caliciviruses (CalVs), coronaviruses (CoVs), flaviviruses, filoviruses, paramyxoviruses (PMVs), polyomaviruses (PYVs) and rotaviruses. We used family-specific primers, amplicon sequencing and further characterization by phylogenetic analysis. Except for filoviruses, eight virus families were detected with varying distributions and …

Production Of Putative Enhanced Oral Cholera Vaccine Strains That Express Toxin-Coregulated Pilus, Caitlyn A. Hauke, Ronald K. Taylor

Dartmouth Scholarship

The use of whole cell killed (WCK) oral cholera vaccines is an important strategy for cholera prevention in endemic areas. To overcome current vaccine limitations, we engineered strains of V. cholerae to be non-toxigenic and to express the protective protein colonization factor, toxin-coregulated pilus (TCP), under scale-up conditions potentially amenable to vaccine production. Two V. cholerae clinical strains were selected and their cholera toxin genes deleted. The tcp operon was placed under control of a rhamnose-inducible promoter. Production and stability of TCP were assessed under various conditions. The strains lack detectable cholera toxin production. The addition of 0.1% …

Recurrent Urinary Tract Infections In Adult Women, Ayaan Mohamed

Nursing Capstones

No abstract provided.

Challenges And Strategies For Prevention Of Multidrug-Resistant Organism Transmission In Nursing Homes, Christopher J. Crnich, Robin L.P. Jump

Faculty Scholarship

Purpose of Review: Nursing home residents are at high risk for colonization and infection with bacterial pathogens that are multidrug-resistant organisms (MDROs). We discuss challenges and potential solutions to support implementing effective infection prevention and control practices in nursing homes. Recent Findings: Challenges include a paucity of evidence that addresses MDRO transmission during the care of nursing home residents, limited staff resources in nursing homes, insufficient infection prevention education in nursing homes, and perceptions by nursing home staff that isolation and contact precautions negatively influence the well being of their residents. Summary: A small number of studies provide evidence that …

Genomic Analysis Of Factors Associated With Low Prevalence Of Antibiotic Resistance In Extraintestinal Pathogenic Escherichia Coli Sequence Type 95 Strains, Craig M. Stephens, Sheila Adams-Sapper, Manraj Sekhon, James R. Johnson, Lee W. Riley

Biology

Extraintestinal pathogenic Escherichia coli (ExPEC) strains belonging to multilocus sequence type 95 (ST95) are globally distributed and a common cause of infections in humans and domestic fowl. ST95 isolates generally show a lower prevalence of acquired antimicrobial resistance than other pandemic ExPEC lineages. We took a genomic approach to identify factors that may underlie reduced resistance. We fully assembled genomes for four ST95 isolates representing the four major fimH-based lineages within ST95 and also analyzed draft-level genomes from another 82 ST95 isolates, largely from the western United States. The fully assembled genomes of antibiotic-resistant isolates carried resistance genes exclusively on …

Misplaced Priorities: How County Health Departments Overlook The Leading Causes Of Death, Jeremy Whitaker

Annual Graduate Student Symposium

Problem: Most Iowans die from chronic diseases, or diseases developed over time, often influenced through behavioral choice. The top three, heart disease, cancer, and lower respiratory diseases account for over half of all death in Iowa and in the nation. However, most public health funding focuses on infectious disease and treatment, leaving little or nothing for primary prevention of chronic diseases.

Method: The 2014 Local Governmental Public Health Survey, annual reports, and Health Improvement projects were analyzed for primary prevention of chronic diseases at county health departments. This was compared to a review of federal and state funding for chronic …

Further Evidence For Bats As The Evolutionary Source Of Middle East Respiratory Syndrome Coronavirus, S. J. Anthony, K. Gilardi, V. D. Menachery

KIP Articles

The evolutionary origins of Middle East respiratory syndrome (MERS) coronavirus (MERS-CoV) are unknown. Current evidence suggests that insectivorous bats are likely to be the original source, as several 2c CoVs have been described from various species in the family Vespertilionidae. Here, we describe a MERS-like CoV identified from a Pipistrellus cf. hesperidus bat sampled in Uganda (strain PREDICT/PDF-2180), further supporting the hypothesis that bats are the evolutionary source of MERS-CoV. Phylogenetic analysis showed that PREDICT/PDF-2180 is closely related to MERS-CoV across much of its genome, consistent with a common ancestry; however, the spike protein was highly divergent (46% amino acid …

Demographic Transition And The Dynamics Of Measles In Six Provinces In China: A Modeling Study, Sheng Li, Chao Ma, Lixin Hao, Qiru Su, Zhijie An, Fubao Ma, Shuyun Xie, Aiqiang Xu, Yangyang Zhang, Zhengrong Ding, Hui Li, Lisa Cairns, Huaqing Wang, Huiming Luo, Ning Wang, Li Li, Matthew J. Ferrari

Publications and Research

Background Industrialization and demographic transition generate nonstationary dynamics in human populations that can affect the transmission and persistence of infectious diseases. Decades of increasing vaccination and development have led to dramatic declines in the global burden of measles, but the virus remains persistent in much of the world. Here we show that a combination of demographic transition, as a result of declining birth rates, and reduced measles prevalence, due to improved vaccination, has shifted the age distribution of susceptibility to measles throughout China.

Methods and findings We fit a novel time-varying catalytic model to three decades of age-specific measles case …

Malaria Over-Diagnosis In Cameroon: Diagnostic Accuracy Of Fluorescence And Staining Technologies (Fast) Malaria Stain And Led Microscopy Versus Giemsa And Bright Field Microscopy Validated By Polymerase Chain Reaction, Sean M. Parsel, Steven A. Gustafson, Edward Friedlander, Alexander A. Shnyra, Aderosoye J. Adegbulu, Ying Liu, Nicole M. Parrish, Syed Jamal, Eve Lofthus, Leo Ayuk, Charles Awasom, Carolyn J. Henry, Carole P. Mcarthur

ETSU Faculty Works

Background: Malaria is a major world health issue and its continued burden is due, in part, to difficulties in the diagnosis of the illness. The World Health Organization recommends confirmatory testing using microscopy-based techniques or rapid diagnostic tests (RDT) for all cases of suspected malaria. In regions where Plasmodium species are indigenous, there are multiple etiologies of fever leading to misdiagnoses, especially in populations where HIV is prevalent and children. To determine the frequency of malaria infection in febrile patients over an 8-month period at the Regional Hospital in Bamenda, Cameroon, we evaluated the clinical efficacy of the Flourescence and …

An Examination Of The Differential Effects Of The Modern Epidemiological Transition On Cranial Morphology In The United States And Portugal, Katherine E. Weisensee, Richard L. Jantz

Human Biology Open Access Pre-Prints

This research examines the pattern of secular change in the cranial morphology of two populations experiencing the epidemiological transition associated with decreased mortality rates in children, followed by declines in infant mortality and subsequent increases in adult longevity. The two samples examined in this study come from US and Portuguese individuals. The epidemiological transition oc- curred at different times in the United States and Portugal, with Portugal entering into the transition later than the United States. The results of the study show that the US and Portuguese samples experienced significant changes in cranial morphology during the approximately 150 years under …

Malaria Over-Diagnosis In Cameroon: Diagnostic Accuracy Of Fluorescence And Staining Technologies (Fast) Malaria Stain And Led Microscopy Versus Giemsa And Bright Field Microscopy Validated By Polymerase Chain Reaction, Sean M. Parsel, Steven A. Gustafson, Edward Friedlander, Alexander A. Shnyra, Aderosoye J. Adegbulu, Ying Liu, Nicole M. Parrish, Syed A. Jamal, Eve Lofthus, Leo Ayuk, Charles Awasom, Carolyn J. Henry, Carole P. Mcarthur

Student Publications

Background: Malaria is a major world health issue and its continued burden is due, in part, to difficulties in the diagnosis of the illness. The World Health Organization recommends confirmatory testing using microscopy-based techniques or rapid diagnostic tests (RDT) for all cases of suspected malaria. In regions where Plasmodium species are indigenous, there are multiple etiologies of fever leading to misdiagnoses, especially in populations where HIV is prevalent and children. To determine the frequency of malaria infection in febrile patients over an 8-month period at the Regional Hospital in Bamenda, Cameroon, we evaluated the clinical efficacy of the Flourescence and …

Jefferson Digital Commons Quarterly Report: January-March 2017, Daniel G. Kipnis, Msi, Kelsey Duinkerken, Msi, James Copeland, Libt

Jefferson Digital Commons Reports

Quarterly report includes highlights of assets added to the JDC along with feedback from researchers.

Alabama Vaccine Research Clinic At Uab Recruiting For Groundbreaking Hiv Prevention Research Trial, Jordan Lessley

Browse All News

No abstract provided.

Seroprevalence Of Poliovirus Antibodies In The Kansas City Metropolitan Area, 2012-2013., Gregory S. Wallace, Barbara A. Pahud, William C. Weldon, Aaron T. Curns, M Steven Oberste, Christopher J. Harrison

Manuscripts, Articles, Book Chapters and Other Papers

No indigenous cases of poliomyelitis have occurred in the US since 1979; however the risk of importation persists until global eradication is achieved. The seropositivity rate for different age cohorts with exposures to different poliovirus vaccine types and wild virus in the US are not presently known. A convenience sample was conducted in the Kansas City metropolitan area during 2012-2103 with approximately 100 participants enrolled for each of 5 age cohorts categorized based on vaccine policy changes over time in the US. Immunization records for poliovirus vaccination were required for participants90.7%, 94.4%, and 83.3%, for types 1, 2, and 3, …

Tenofovir Inhibits Wound Healing Of Epithelial Cells And Fibroblasts From The Upper And Lower Human Female Reproductive Tract, Marta Rodriguez-Garcia, Mickey V. Patel, Zheng Shen, Jack Bodwell, Richard M. Rossol, Charles R. Wira

Dartmouth Scholarship

Disruption of the epithelium in the female reproductive tract (FRT) is hypothesized to increase HIV infection risk by interfering with barrier protection and facilitating HIV-target cell recruitment. Here we determined whether Tenofovir (TFV), used vaginally in HIV prevention trials, and Tenofovir alafenamide (TAF), an improved prodrug of TFV, interfere with wound healing in the human FRT. TFV treatment of primary epithelial cells and fibroblasts from the endometrium (EM), endocervix (CX) and ectocervix (ECX) significantly delayed wound closure. Reestablishment of tight junctions was compromised in EM and CX epithelial cells even after wound closure occurred. In contrast, TAF had no inhibitory …

Strategies For Reducing Preventable Hospital Readmissions On Medicare Patients, Andres Patricio Garcia-Arce

USF Tampa Graduate Theses and Dissertations

The high expenditure of healthcare in the United States (U.S.) does not translate into better quality of care. Indeed, the U.S. healthcare system is recognized by its lack of efficiency and waste (which represents about 20% of the country’s healthcare expenses). Lack of coordination is one of the most referenced causes of waste in the U.S. healthcare system, and preventable hospital readmissions have been acknowledged to be evidence of poor coordination of care. In fiscal year 2013, the Centers for Medicare and Medicaid Services (CMS) established financial penalties for inpatient care reimbursements in hospitals with excessive readmissions. All the same, …

Dynamics Of Multicultural Social Networks, Kristina B. Hilton

USF Tampa Graduate Theses and Dissertations

Historically human endeavors around the globe are in search of bilateral relationships. Knowledge and commerce has played a very significant role in increasing the ability for humans to connect for the betterment of the human species. As the means of communication improve, mutual benefits to the community as a whole also increase. Moreover, the benefits are filtered down to members of the overall community. Recent advancement in electronic communication technologies and in knowledge, in particular, physical, chemical, engineering and medical sciences and philosophies, have facilitated nearly instantaneous multi-cultural interactions. Local problems and solutions have become global. This has generated a …

Seasonality Of Auricular Amputations In Rabbits, Kathleen Yaremchuk, Vigen Darian, Amy M. Williams

Otolaryngology Articles

This retrospective observational analysis hypothesizes that an increase occurs in online reports and images of auricular amputations of confectionary rabbits during the spring. Using the online search engine Google, online content and visual portrayals of confectionary rabbit auricular amputations from 2012 to 2017 were identified and trended against seasonal variations. To determine incidence, commercial availability of chocolate rabbits in retail facilities were assayed. A statistically significant increase in mention of rabbit auricular amputations occurred during the spring. Mapping techniques showed the annual peak incidence for 2012 to 2017 to be near Easter for each year studied. Human adults and children …

Commercial Deception By Anti-Vaccine Homeopathic Websites: A Consumer Protection Approach, Donald C. Arthur

North Carolina Central University Science & Intellectual Property Law Review

No abstract provided.

Law And Health Care Newsletter, Spring 2017

Law & Health Care Newsletter

No abstract provided.

Crispr/Cas9 Genome Editing System And Its Use In Infectious Disease, Dustin Daws

Thinking Matters Symposium Archive

Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) is an exciting new genetic engineering technology that was found in the chromosomes of certain bacteria and archaea. Bacteriophage are viruses that insert their DNA and hijack the host cell’s machinery to make new phage that can go on to infect new cells. Some microorganisms use CRISPR as a defense mechanism to disrupt the bacteriophage DNA after it is inserted into the cell. CRISPR/Cas9 uses genome editing as a means to alter very specific sections of a foreign genome. CRISPR works with a RNA-guided DNA endonuclease called Cas9. This enzyme can locate and …

Volume 41, Issue 1 (Spring 2017)

Transcript

No abstract provided.

Wellesley Magazine Spring 2017, Wellesley College Alumnae Association

Wellesley Magazine (Alumnae Association)

Featured in this issue:

Opening the Cloister / by Catherine O’Neill Grace

Curatorially Speaking / by Lisa Scanlon Mogolov ’99

The Davis Rediscovered / by April Austin

The Hidden Truth / by Julie Catterson Lindahl ’88

Is Addressing Climate Change Women's Work? Political Leadership And The Climate, Isabella Soparkar

Environmental Studies Honors Projects

In an era when climate science is politically controversial, recent polling data shows that American women are more concerned about climate change than their male counterparts. This research uses both voting record analysis and qualitative interviews with legislators to examine whether the observed gender gap on climate change persists among elected political leaders. Linear and logistic regression results show no statistically significant climate change gender gap within legislative voting behavior, and interviews suggest that though women may be more willing to collaborate on climate change policy than men, subtle gender differences are often overridden by partisanship. However, findings suggest that …

Prevention Of Drug Use And Treatment Of Drug Use Disorders In Rural Settings, John A. Gale Ms

Mental Health / Substance Use Disorders

This Guide on Prevention of Drug Use and Treatment of Drug Use Disorders in Rural Settings was prepared by the United Nations Office on Drugs and Crime (UNODC) Drug Prevention and Health Branch (DHB), in the context of the global project Treatnet II: OFID-UNODC Programme to prevent HIV/AIDS through Treatnet Phase II, with the aim of providing an awareness-raising tool and guidance for policymakers, public health officials, local authorities and other stakeholders in dealing with substance use issues in rural settings in their respective countries. This Guide will serve as an awareness-raising tool and guidance for policymakers, public health officials, …

Gallaudet Today Volume 47 Number 1 Spring 2017, Phil Dignan

Gallaudet Today

No abstract provided.

Co-Occurrence Patterns Of Bat Flies On Neotropical Chiroptera, Mitchell Louis Schooler

Masters Theses & Specialist Projects

Parasite-host systems provide excellent opportunities to explore ecological dynamics such as competition, competitive exclusion, and co-occurrence. The distribution of streblid bat flies on their host bats were examined for patterns of species co-occurrence and to understand mechanisms driving these patterns. The purpose of this study was to determine patterns of co-occurrence among individuals of different Neotropical bat fly species. After establishing patterns of co-occurrence, tests on whether variation in fly morphology was linked to observed patterns of co-occurrence were performed. Co-occurrence patterns were determined using null model analyses, and a predominant pattern of aggregation was detected. To examine the relationship …

The Influence Of Culture On The Use Of Healthcare Services By Refugees In Southcentral Kentucky: A Mixed Study, Chika N. Ejike

Dissertations

The world as a global village has become a ubiquitous trope in the popular discourse, and Bowling Green, Kentucky, with its substantial immigrant population, may be considered an exemplar of this idealized community. It has become an ideal location for research regarding the challenges faced by immigrants. Due to the diverse cultural identities of the refugee/immigrant population, it is particularly well suited for studies into complex culturally dependent healthcare utilization patterns.

The central research question for the study was as follows: What are the healthcare-seeking behavioral patterns (as influenced by culture) among refugees at their nearest healthcare facilities? This mixed …

Solid Waste Management In The Developing World: The Role Of Local Government In Kisumu, Kenya, Rachel Schlueter

Independent Study Project (ISP) Collection

As the world becomes more urbanized, more waste is being produced. According to United Nations projections in 2014, 66% of the world’s population will be living in cities by 2050 with the majority of growth occurring in African cities. Managing urban waste in lower-middle income countries, such as Kenya, poses a particular challenge as income levels rise, municipal capacities are stretched, and foreign loans complicate accountability. This project sought to assess the practical role of local government when managing solid waste in the context of long-term development strategies. The objectives of this study are to first establish the current role …