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Microfluidic Cantilever Detects Bacteria And Measures Their Susceptibility To Antibiotics In Small Confined Volumes, Hashem Etayash, M. F. Khan, Kamaljit Kaur, Thomas Thundat

Pharmacy Faculty Articles and Research

In the fight against drug-resistant bacteria, accurate and high-throughput detection is essential. Here, a bimaterial microcantilever with an embedded microfluidic channel with internal surfaces chemically or physically functionalized with receptors selectively captures the bacteria passing through the channel. Bacterial adsorption inside the cantilever results in changes in the resonance frequency (mass) and cantilever deflection (adsorption stress). The excitation of trapped bacteria using infrared radiation (IR) causes the cantilever to deflect in proportion to the infrared absorption of the bacteria, providing a nanomechanical infrared spectrum for selective identification. We demonstrate the in situ detection and discrimination of Listeria monocytogenes at a …

First Direct Evidence Of Long-Distance Seasonal Movements And Hibernation In A Migratory Bat, Theodore J. Weller, Kevin T. Castle, Felix Liechti

KIP Articles

Understanding of migration in small bats has been constrained by limitations of techniques that were labor-intensive, provided coarse levels of resolution, or were limited to population-level inferences. Knowledge of movements and behaviors of individual bats have been unknowable because of limitations in size of tracking devices and methods to attach them for long periods. We used sutures to attach miniature global positioning system (GPS) tags and data loggers that recorded light levels, activity, and temperature to male hoary bats (Lasiurus cinereus). Results from recovered GPS tags illustrated profound differences among movement patterns by individuals, including one that completed a >1000 …

Palaeoproteomic Evidence Identifies Archaic Hominins Associated With The Châtelperronian At The Grotte Du Renne, Frido Welker, Mateja Hajdinjak, Sahra Talamo

KIP Articles

In Western Europe, the Middle to Upper Paleolithic transition is associated with the disappearance of Neandertals and the spread of anatomically modern humans (AMHs). Current chronological, behavioral, and biological models of this transitional period hinge on the Châtelperronian technocomplex. At the site of the Grotte du Renne, Arcy-sur-Cure, morphological Neandertal specimens are not directly dated but are contextually associated with the Châtelperronian, which contains bone points and beads. The association between Neandertals and this “transitional” assemblage has been controversial because of the lack either of a direct hominin radiocarbon date or of molecular confirmation of the Neandertal affiliation. Here we …

Beryllium Nitrate Inhibits Fibroblast Migration To Disrupt Epimorphic Regeneration, Adam B. Cook, Ashley W. Seifert

Biology Faculty Publications

Epimorphic regeneration proceeds with or without formation of a blastema, as observed for the limb and skin, respectively. Inhibition of epimorphic regeneration provides a means to interrogate the cellular and molecular mechanisms that regulate it. In this study, we show that exposing amputated limbs to beryllium nitrate disrupts blastema formation and causes severe patterning defects in limb regeneration. In contrast, exposing full-thickness skin wounds to beryllium only causes a delay in skin regeneration. By transplanting full-thickness skin from ubiquitous GFP-expressing axolotls to wild-type hosts, we demonstrate that beryllium inhibits fibroblast migration during limb and skin regeneration in vivo. Moreover, …

Minutes Of Graduate College Committee On Curricula, October 4, 2016, University Of Northern Iowa. Graduate College Curriculum Committee.

Minutes - Graduate Curriculum Committee

Minutes of the committee that oversees the development of the curriculum of the University of Northern Iowa Graduate College.

Soil Salinity Increases The Tolerance Of Excessive Sulfur Fumigation Stress In Tomato Plants, Yuping Jiang, Xiaotao Ding, Dong Zhang, Qi Deng, Chih-Li Yu, Suping Zhou, Dafeng Hui

Agricultural and Environmental Sciences Faculty Research

To investigate the responses of plant photosynthesis and chlorophyll fluorescence to excessive sulfur fumigation stress (Sulfur) alleviated by pre-treated salt in the soil (Salt), seedlings of a tomato cultivar (Solanum lycopersicum L. ‘Money Maker’) were exposed to the Sulfur with or without the Salt treatment for 15h. Leaf fresh weight, dry weight, chlorophyll (Chl) content, carotenoid (Car) content, photosynthesis and chlorophyll fluorescence parameters were measured. The results showed that the Sulfur treatment significantly decreased leaf fresh weight, dry weight, Chl and Car contents, net photosynthetic rate (Pn), stomatal conductance (Gs), transpiration rate (Tr), maximum quantum efficiency of photosystem II (PSII) …

Unh Kicks Off 150th Anniversary, Erika Mantz

UNH Today Archive

No abstract provided.

Minutes, Curriculum Committee Meeting, Tuesday, October 4, 2016, Curriculum Committee

Curriculum Committee Minutes

No abstract provided.

Effective Treatment Of Esophageal Adenocarcinoma Using Triciribine And Related Compounds, Jing Q. Cheng

USF Patents

The inventors have determined, contrary to the prior art and experience, how to successfully use triciribine to treat esophogeal adenocarcinoma by one or a combination of (i) administering triciribine only to patients which according to a diagnostic test described below, exhibit enhanced sensitivity to the drug; (ii) use of a described dosage level that minimizes the toxicity of the drug but yet still exhibits efficacy; or (iii) use of a described dosage regimen that minimizes the toxicity of the drug.

Compositions And Methods For Adipocyte Modulation, Niketa A. Patel, Rekha S. Patel, Kirpal S. Bisht

USF Patents

Modified stilbenoids and formulations thereof are provided. The modified stilbenoids can include one or more amino or amino-alkyl substituents. The formulation can be for the treatment or prevention of obesity and the modified stilbenoid present in an effective amount to alleviate or prevent the onset of one or more symptoms of obesity. Methods of making the modified stilbenoids and formulations thereof are provided. Methods are provided for treating a subject with a modified stilbenoid in an effective amount to alleviate or prevent one or more symptoms associated with diabetes, cardiovascular disease, high blood pressure, deep vein thrombosis, osteoarthritis, obstructive sleep …

Brief Amici Curiae Of Professors Of History, Political Science, And Law In Support Of Respondent, Kristin Collins, Catherine E. Stetson, Jessica K. Jacobs

Faculty Scholarship

Sex-based laws premised on archaic presumptions about the proper roles of men and women run afoul of established constitutional principles, especially when they interfere with the parent-child relationship. Amici write to explain the history of the federal government’s use of sex-based classifications in the regulation of citizenship. In its regulation of intergenerational and interspousal citizenship transmission, the federal government has perpetuated outdated gender-based norms concerning proper parental roles, even when those norms have been rejected in other legal and social contexts. In addition, the laws governing derivative citizenship have significantly encumbered the ability of American fathers to transmit citizenship to …

Negative Cooperativity In The Nitrogenase Fe Protein Electron Delivery Cycle, Karamatullah Danyal, Sudipta Shaw, Taylor R. Page, Simon Duval, Masaki Horitani, Amy R. Marts, Dmitriy Lukoyanov, Dennis R. Dean, Simone Raugei, Brian M. Hoffman, Lance C. Seefeldt, Edwin Antony

Biological Sciences Faculty Research and Publications

Nitrogenase catalyzes the ATP-dependent reduction of dinitrogen (N₂) to two ammonia (NH₃) molecules through the participation of its two protein components, the MoFe and Fe proteins. Electron transfer (ET) from the Fe protein to the catalytic MoFe protein involves a series of synchronized events requiring the transient association of one Fe protein with each αβ half of the α₂β₂ MoFe protein. This process is referred to as the Fe protein cycle and includes binding of two ATP to an Fe protein, association of an Fe protein with the MoFe protein, ET from the …

The Current Volume 27 : Issue 7, Nova Southeastern University

Student Newspapers

No abstract provided.

The George-Anne, Georgia Southern University

The George-Anne

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Distinct Cell Stress Responses Induced By Atp Restriction In Quiescent Human Fibroblasts., Nirupama Yalamanchili, Andres Kriete, David Alfego, Kelli M Danowski, Csaba Kari, Ulrich Rodeck

Department of Dermatology and Cutaneous Biology Faculty Papers

Quiescence is the prevailing state of many cell types under homeostatic conditions. Yet, surprisingly little is known about how quiescent cells respond to energetic and metabolic challenges. To better understand compensatory responses of quiescent cells to metabolic stress, we established, in human primary dermal fibroblasts, an experimental 'energy restriction' model. Quiescence was achieved by short-term culture in serum-deprived media and ATP supply restricted using a combination of glucose transport inhibitors and mitochondrial uncouplers. In aggregate, these measures led to markedly reduced intracellular ATP levels while not compromising cell viability over the observation period of 48 h. Analysis of the transcription …

Applied Photoproperties Of Phenylene Ethynylenes, Patrick L. Donabedian

Nanoscience and Microsystems ETDs

Light-absorbing molecules can be used as powerful tools to perturb and understand biological systems by fluorescence, sensitization, or photochemical reactions. A thorough understanding of the delivery of dyes to specific biochemical targets and the processes that control the fate of excited-state energy is needed to engineer useful technology out of organic photochemistry. This thesis presents four projects investigating different aspects of pathogen destruction and biochemical sensing in a variety of systems, using the properties of p-phenylene ethynylenes (PEs), an especially flexible and well-studied class of conjugated molecules. Of particular relevance, some PEs are found to be effective dyes for amyloid …

Membrane Biophysics Of Listeria Monocytogenes: Analysis Of An Alternate Pathway Of Branched-Chain Fatty Acid Biosynthesis And Elasticity Of Fatty Acid Utilization, Laura E. Kuczek

Theses and Dissertations

The membrane homeostasis of the psychrotolerant foodborne pathogen Listeria monocytogenes involves maintaining fluidity in a range of different conditions by incorporation of specific branched-chain fatty acids (BCFAs). BCFAs are synthesized through a well-characterized pathway from branched-chain amino acids into short branched-chain acyl-CoAs followed by elongation by the FAS II system. Branched-chain alpha-keto acid dehydrogenase (Bkd) is a major player in this pathway. MOR401 is a transposon insertion mutant deficient in Bkd and has decreased membrane BFCAs. Low levels of BCFAs in L. monocytogenes are linked to diminished growth, less resistance to antimicrobials, and a severe reduction in virulence. Rescued growth …

Fhf2 Gene Deletion Causes Temperature-Sensitive Cardiac Conduction Failure, David S. Park, Akshay Shekhar, Christopher Marra, Xianming Lin, Carolina Vasquez, Sergio Solinas, Kevin Kelley, Gregory Morley, Mitchell Goldfarb, Glenn I. Fishman

Publications and Research

Fever is a highly conserved systemic response to infection dating back over 600 million years. Although conferring a survival benefit, fever can negatively impact the function of excitable tissues, such as the heart, producing cardiac arrhythmias. Here we show that mice lacking fibroblast growth factor homologous factor 2 (FHF2) have normal cardiac rhythm at baseline, but increasing core body temperature by as little as 3 C causes coved-type STelevations and progressive conduction failure that is fully reversible upon return to normothermia. FHF2-deficient cardiomyocytes generate action potentials upon current injection at 25 C but are unexcitable at 40 C. The absence …

Lessons From The Far End: Caterpillar Frass-Induced Defenses In Maize, Rice, Cabbage, And Tomato, Swayamjit Ray, Saumik Basu, Loren J. Rivera-Vega, Flor E. Acevedo, Joe Louis, Gary W. Felton, Dawn S. Luthe

Department of Entomology: Faculty Publications

Plant defenses to insect herbivores have been studied in response to several insect behaviors on plants such as feeding, crawling, and oviposition. However, we have only scratched the surface about how insect feces induce plant defenses. In this study, we measured frass-induced plant defenses in maize, rice, cabbage, and tomato by chewing herbivores such as European corn borer (ECB), fall armyworm (FAW), cabbage looper (CL), and tomato fruit worm (TFW). We observed that caterpillar frass induced plant defenses are specific to each host-herbivore system, and they may induce herbivore or pathogen defense responses in the host plant depending on the …

Evidence For Energy Conservation During Pubertal Growth. A 10-Year Longitudinal Study (Earlybird 71), M Mostazir, A Jeffery, J Hosking, B Metcalf, L Voss, T Wilkin

Peninsula Medical School

No abstract provided.

The Bg News October 4, 2016, Bowling Green State University

BG News (Student Newspaper)

The BGSU campus student newspaper. October 4, 2016. Volume 96-Issue 14.

The Effect Of Confirmation Bias In Criminal Investigative Decision Making, Wayne A. Wallace

Harold L. Hodgkinson Award for Outstanding Dissertation

Confirmation bias occurs when a person believes in or searches for evidence to support his or her favored theory while ignoring or excusing disconfirmatory evidence and is disinclined to change his or her belief once he or she arrives at a conclusion. The purpose of this quantitative study was to examine whether emotionally charged evidence and evidence presentation order could influence an investigator's belief in a suspect's guilt. The study included 166 sworn police officers (basic training recruits, patrol officers, and criminal investigators) who completed online surveys in response to criminal vignettes across different scenarios to record their measure of …

"I'M Man Enough: Are You?": The Queer (Im)Possibilities Of Walk A Mile In Her Shoes

Journal of Critical Scholarship on Higher Education and Student Affairs

Walk a Mile in Her Shoes is a national program that has become a staple program to engage college males in sexual violence prevention on many college campuses. In this manuscript, I use queer theory and crip theory—a conceptual framework that merges queer and critical disability theory—to explore both the positive outcomes and potential harm done in the production and implementation of this event. I conclude the manuscript with considerations for educators seeking to engage college students in critical praxis around ending sexual violence on campus. These possibilities are rooted in Cohen's (1998) notion of reorienting future praxis around the …

National Wildlife Federation V. National Marine Fisheries Service, Jacob R. Schwaller

Public Land & Resources Law Review

The tide in the legal battle surrounding anadromous fish protections in the Columbia River watershed most recently swung in favor of the fish. In the latest iteration of National Wildlife Federation v. National Marine Fisheries Service, the Court found in a lengthy opinion that NOAA fisheries acted arbitrarily and capriciously when it issued its 2014 BiOp concluding that the FCRPS did not violate the ESA. The Court also ruled that the Corps violated NEPA by failing to prepare an environmental impact statement in connection with their records of decision implementing reasonable and prudent alternatives in the BiOp. This decision could …

Carotid Artery Imt, Blood Pressure, And Cardiovascular Risk Factors In Males And Females, Christina M. Cromwell, Kristin R. Aichele, Joyann E. Oakman, Michael P. Neal, Jessica M. Lenzo, Avery N. Perez, Naomi L. Bye, Erica L. Santaniello, Jessica A. Hill, Rachel C. Evans, Karla A. Thiele, Lauren N. Chavis, Allyson K. Getty, Tia R. Wisdo, Deborah L. Feairheller, Phd

International Journal of Exercise Science

International Journal of Exercise Science 9(4): 482-490, 2016. Previous studies have investigated carotid artery intima-media thickness (IMT) and blood pressure and found a direct correlation between the two. It is known that adult females have better cardiovascular health than males until a certain stage of life, yet limited research has examined gender differences in vascular function. Thus, the purpose of this study was to investigate vascular structure and function, blood pressure, and blood glucose/cholesterol levels in relation to gender differences in young healthy adults. On three separate days, 44 adults (26.30 ±11.9yrs; 24M, 20F) completed a carotid IMT ultrasound, a …

Physical Fitness And Self-Image: An Evaluation Of The Exercise Self-Schema Questionnaire Using Direct Measures Of Physical Fitness, Jafra D. Thomas, J. Mark Vanness, Bradley J. Cardinal

International Journal of Exercise Science

International Journal of Exercise Science 9(4): 445-459, 2016. The purpose of this study was to perform a construct validity assessment of Kendzierski’s exercise self-schema theory questionnaire using objective measures of health-related physical fitness. This study tested the hypothesis that individuals with an exercise self-schema would possess significantly greater physical fitness than those who did not across three domains of health-related physical fitness: Body composition, cardiovascular fitness, and upper-body muscular endurance. Undergraduate student participants from one private university on the west coast of the United States completed informed consent forms and the exercise self-schema questionnaire within a classroom setting or at …

Adolescents’ Perception Of The Psychosocial Factors Affecting Sustained Engagement In Sports And Physical Activity, James Gavin, Madeleine Mcbrearty, Kit Malo, Michael Abravanel, Tatiana Moudrakovski

International Journal of Exercise Science

International Journal of Exercise Science 9(4): 384-411, 2016. The purpose of this study was to explore adolescents’ perceptions of psychosocial influences – personal characteristics, environmental factors and behavioural undertakings – influencing their prolonged involvement in sports and physical activity (PA). A qualitative approach was adopted wherein 16 adolescents (8 boys, 8 girls; mean age 15.9 years), who had been physically active for at least the last 8 years, and sixteen adults identified as their ‘parents’ or ‘guardians’ participated in semi-structured interviews. Interviews were transcribed verbatim and coded using the HyperRESEARCH software. Data were analysed using thematic analysis procedures. Four main …

Access To The Safe Harbor: Bioterrorism, Influenza, And The Supreme Court's Interpretation Of The Research Exemption From Patent Infringement, Michael J. Brignati

Journal of Intellectual Property Law

No abstract provided.

The Biotechnology Process Patent Act Of 1995: Providing Unresolved And Unrecognized Dilemmas In U.S. Patent Law, Becca Alley

Journal of Intellectual Property Law

No abstract provided.

University Research-A New Defense Under The Patent Law, Judith L. Curry, Bruce E. O'Connor

Journal of Intellectual Property Law

No abstract provided.