Digital Commons Network^™

Sleep Disorder Trends, Epigenetic Markers, And Genetic Variation Of Circadian Genes In Adenomatous Polyp Formation, Melannie Alexander

Theses and Dissertations

Introduction: Sleep disturbances and sleep quality have fluctuated over time in the United States (US). Virtually every physiological process follows a circadian pattern, and cellular processes (i.e., cell proliferation, DNA damage response, and apoptosis) are often disrupted by sleep disturbances and other factors, potentially giving rise to adverse health outcomes such as cancer. Additionally, both genetic variation and epigenetic patterns within clock genes, which may affect sleep quality, also may increase one’s risk for developing cancer. The role of sleep disturbances in the formation of adenomas, precursor lesions to colorectal cancer (CRC), has been explored previously; however, little is …

Registration And Clustering Of Functional Observations, Zizhen Wu

Theses and Dissertations

As an important exploratory analysis, curves of similar shape are often classified into groups, which we call clustering of functional data. Phase variations or time distortions are often encountered in the biological processes, such as growth patterns or gene profiles. As a result of time distortion, curves of similar shape may not be aligned. Regular clustering methods for functional data usually ignore the presence of phase variations, which may result in low clustering accuracy. However, it is difficult to account for phase variation without knowing the cluster structure.

In this dissertation, we first propose a Bayesian method that simultaneously clusters …

Regulation Of Chronic And Acute Inflammatory Disease By Microrna And Microbiota, Pegah Mehrpouya-Bahrami

Theses and Dissertations

Inflammation is implicated in cancer development, degenerative diseases, allergies as well as artherosecelorsis. Dysregulated immune responses lead to chronic inflammation and tissue damage. Finding the ways to terminate inflammatory responses when no longer needed, demands further investigation. Herein, we investigated the modulation of acute and chronic inflammatory disease models by inducing antiinflammatory state. Acute inflammatory model was induced with SEB, an enterotoxin produced by a ubiquitous Gram-positive coccus, Staphylococcus aureus (S. aureus), which exerts profound toxic effects on the immune system, which leads to the cytokine storm and adverse immune response. SEB is the main cause of nosocomial infections, acute …

Development And Application Of Foraminiferal Carbonate System Proxies To Quantify Ocean Acidification In The California Current, Emily B. Osborne

Theses and Dissertations

The oceanic uptake of anthropogenic carbon has mitigated climate change, but has also resulted in a global average 0.1 decline in surface ocean pH over 20th century known as ocean acidification. The parallel reduction in carbonate ion concentration ([CO32-]) and the saturation state of seawater (Ω) has caused many major calcium carbonate-secreting organisms such as planktonic foraminifera to exhibit impaired calcification. We develop proxy calibrations and down core records that use calcification and geochemical characteristics of planktonic foraminifera as proxies for the marine carbonate system. This study focuses specifically on the surface ocean chemistry of the California Current Ecosystem (CCE), …

Assessing Individual Transit Vulnerability To Nuisance Flooding In The Charleston, Sc Area, Sumathee Selvaraj

Theses and Dissertations

Minor coastal flooding, also known as nuisance flooding, is projected to be more frequent due to relative sea level rise. Nuisance flood events in Charleston have resulted in various social impacts caused by road closures, traffic disruptions, and economic losses. This thesis presents research conducted to understand the dimensions of individual transit vulnerability to nuisance flooding and how transit vulnerability will be affected by increased extents of nuisance flooding driven by rising sea levels and heavy rainfall. Mixed methods were used to conduct this research in Berkley, Charleston, and Dorchester Counties, South Carolina. An electronic, in-person survey was administered at …

Exploratory Analysis Of Ptsd Severity And Objective Measures Of Physical Activity Among Combat Veterans, Danny O. Sauceda

Theses and Dissertations

Strong levels of motivation, relatedness and self-determination support the likeliness of participating in habitual exercise. Compared to nationwide estimates, research has demonstrated fitness and performance is significantly lower among those suffering from PTSD. Meeting established recommended daily levels of physical activity (PA) for individuals with PTSD is critical for their physical and mental health status over a lifetime and factors supporting or hindering adherence to habitual exercise are lacking. Furthermore, regular PA among combat Veterans with Posttraumatic Stress Disorder (PTSD) is a viable and substantial treatment option to improve physical health status and cognitive well-being. The purpose of this exploratory …

Hydro-Geological Flow Analysis Using Hidden Markov Models, Chandrahas Raj Venkat Gurram

Theses and Dissertations

Hidden Markov Models a class of statistical models used in various disciplines for understanding speech, finding different types of genes responsible for cancer and much more. In this thesis, Hidden Markov Models are used to obtain hidden states that can correlate the flow changes in the Wakulla Spring Cave. Sensors installed in the tunnels of Wakulla Spring Cave have recorded huge correlated changes in the water flows at numerous tunnels. Assuming the correlated flow changes are a consequence of system being in a set of discrete states, a Hidden Markov Model is calculated. This model comprising all the sensors installed …

Structural, Functional, And Behavioral Alterations To The Dopamine System In The Female Hiv-1 Transgenic Rat, Robert Francis Roscoe Jr.

Theses and Dissertations

HIV-associated cognitive disorders continue to affect approximately 25 million individuals worldwide, and its prevalence is expected to increase as the lifespan of HIV-1 infected individuals continues to improve. The HIV-1 transgenic rat expresses 7 of the 9 genes that encompass the virus, and is an appropriate model for studying chronic HIV-1 infection at a level that is controlled through combined antiretroviral therapy. Psychostimulant abuse is known to exacerbate HAND symptomology, but the psychostimulant methylphenidate is a first line of treatment for HIV associated cognitive inhibition. Dopamine transport abnormalities in the HIV-1 Tg rat have been well characterized through behavioral testing; …

Depression Among African American Midlife Women: Delineating The Role Of Stress, Heather Page

Theses and Dissertations

Recent multi-ethnic epidemiologic studies indicate an increased risk for the onset of new as well as recurrent episodes of clinical depression among midlife women. However, little is known about the risk factors that influence the occurrence, severity, and course of depression in midlife among African American women (AAW). The purpose of the current study was to statistically model three prevailing conceptual views of stress and depression: 1) life course, 2) stress exposure, and 3) stress generation). The research investigated the abilities of these conceptual models to predict depressive symptoms severity, current depressed days, and major depression among midlife AAW. Data …

Applications Of Disposable Pipette Technologies With Lc-Ms/Ms For Forensic And Clinical Analyses Of Biological Matrices, Kaylee R. Mastrianni

Theses and Dissertations

Efficiently hydrolyzing glucuronide metabolites is an important step in the analysis of drugs in urine. Amitriptyline and cyclobenzaprine are both tricyclic compounds with tertiary aliphatic amine groups that are subsequently metabolized to a less common form of glucuronide metabolite, quaternary ammonium linked glucuronides (N+- glucuronide). A collaborative study was conducted to investigate discrepancies in recoveries of these two commonly prescribed compounds in patient urine samples when hydrolyzed with different enzyme from four different commercially available β-glucuronidase sources.

Similarly, there is potential for 6-monoacetylmorphine to be converted to morphine during β-glucuronidase hydrolysis of urine samples. 6-monoacetylmorphine is a unique metabolite of …

Biodegradable Hybrid Tissue Engineering Scaffolds For Reconstruction Of Large Bone Defects, Danial Barati

Theses and Dissertations

Complex skeletal injuries and large bone fractures are still a significant clinical problem in US. Approximately 1.5 million Americans (veterans, their families, and civilians) every year suffer from bone loss due to traumatic skeletal injuries, infection, and resection of primary tumors that require extensive grafting to bridge the gap. The US bone graft market is over $2.2 billion a year. Due to insufficient mechanical stability, lack of vascularity, and inadequate resorption of the graft, patients with traumatic large skeletal injuries undergo multiple costly operations followed by extensive recovery steps to maintain proper bone alignment and length. Current strategies for repairing …

Regular Expression Synthesis For Blast Two-Hit Filtering, Jordan Bradshaw

Theses and Dissertations

Genomic databases are exhibiting a growth rate that is outpacing Moore's Law, which has made database search algorithms a popular application for use on emerging processor technologies. NCBI BLAST is the standard tool for performing searches against these databases, which operates by transforming each database query into a filter that is subsequently applied to the database. This requires a database scan for every query, fundamentally limiting its performance by I/O bandwidth. In this dissertation we present a functionally-equivalent variation on the NCBI BLAST algorithm that maps more suitably to an FPGA implementation. This variation of the algorithm attempts to reduce …

Ligand Engineering For Advanced Functional Composite Materials, Michael H. Bell

Theses and Dissertations

The surface modification of solid inorganic substrates for thermodynamic compatibility with organic polymer media has become an important area of study for the development of advanced functional composite materials. Polymer ligands covalently bound to the substrate surface have been an effective means to achieve said compatibility. Current investigations focus on multifunctional ligand engineering where multiple chemically distinct species, each with specific functionality, can be implemented on the filler surface simultaneously. A combination of matrix compatible polymer brushes and conjugated charge trapping moieties attached to filler surfaces are investigated for enhancements in dielectric properties. Presented herein are the synthesis and characterization …

Mislabeling Of Commercial Atlantic Cod (Gadus Morhua) Products In Spain, Joshua Helgoe

Theses and Dissertations

The mislabeling of commercial fish products is a pervasive, worldwide problem. Most consumers of seafood are unaware this issue directly affects them and can even have negative impacts on their health. Mislabeling occurs when a product’s label is inconsistent with its content. Although mislabeling can be unintentional, deliberate mislabeling is a more common trend to increase profits and/or bypass fishing regulations - a form of economic fraud. Unfortunately, oversight, enforcement, and research are vastly insufficient in relation to the global scale of the problem. In order to add to the small knowledge base on European mislabeling rates, determine if overfished …

Estimation Of The Quantum Effects Of Nuclei In Large Molecular Systems, Bing Gu

Theses and Dissertations

Chemical dynamics, in principle, should be understood by solving the time-dependent Schrödinger equation for a molecular system, describing motion of the nuclei and electrons. However, the computational efforts to solve this partial second-order differential equation scales exponentially with the system size, which prevents us from getting exact numerical solutions for systems larger than 4-5 atoms. Thus, approximations simplifying the picture are necessary. The so-called Born-Oppenheimer approximation, separating motion of the electrons and nuclei is the central one: solution to the electronic Schrödinger equation defines the potential energy surface on which the nuclear motion unfolds, and there are standard quantum chemistry …

Synthesis Attempts And Plausible Applications Of Dithiolated Ortho –Carboranyl Transition Metal Complexes And Synthesis Of Bulky Terphenyls, Surendra Bahadur Karki

Theses and Dissertations

The reaction of coordination compounds of palladium, rhodium, ruthenium and nickel with thiolated ortho-carborane derivatives- 1,2-dimercapto-o-carborane (1,2-(HS)2-1,2- C2B10H10 ) and 9,12- dimercapto-o-carborane (9,12-(HS)2-1,2- C2B10H10 ) and with carbornanyl dicarboxylate has been studied. To further investigate the chemistry of o-carboranyl dithiolate, both of the reported literature dithiols have been employed to study the reactivity with some of the above mentioned transition metal coordination compounds. The 1,2-dimercapto-o-carborane reacts with bis(triphenylphosphine)palladium(II) chloride [PdCl2(PPh3)2] to form a square planar bis(triphenylphosphine)palladium(II) carboranyl dithiolate (PdC2B10S2(PPh3)2) complex. With the inspiration from the reactivity of the dithiols and versatility of carboranyldithiolate ligand in organometallic chemistry, new avenue of …

In The Belly Of The Whale: Archive And Access In Melville’S Moby-Dick, Garrett Urban

Theses and Dissertations

This thesis examines Melville’s depiction of archival anxiety as it appears in Moby-Dick. This narrative is fixated on the archive, brimming with motifs that equate whaling with the creation of an archive—and which associates the hunt for the whale with an obsession resembling Derrida’s vision of archive fever. As Melville depicts it, the archive is rooted in a fundamental anxiety concerning inaccessible and unknowable information. Ishmael constantly presents whales as physical repositories of information, attempting to archive the social and historical contexts of every scrap of flesh on them and every substance they produce. Yet his archival mission is perpetually …

Business And Security In The Age Of Terrorism: The Long-Term Effects Of The September 11 Terrorist Attacks On Seaport Governance And Control, Daniel J. Ostergaard

Theses and Dissertations

The primary research goal of this dissertation is to improve our theoretical understanding of the longterm effects of major transnational terrorist events on securityrelated institutional changes for business. To accomplish this goal, the September, 11, 2001, terror attack was chosen as the starting point for this research. The research context is the governance of seaport security. Using grounded theory to develop comparative case studies of several major security initiatives in the maritime industry after 9/11, this dissertation shows that a terrorist event initiates a process of institutional change that emerges over time as initial securityrelated ideas cascade into concrete actions. …

Orange Juice Elegies, Jared Coffin

Theses and Dissertations

This is a creative thesis presented as a collection of poems written by Jared Coffin. This is the complete thesis submitted, defended, and approved as part of Jared’s completion of the requirements for earning the Master of Fine Arts in Creative Writing.

Cobaltocenium Containing Polymers: Complexation With Organic Anion And Initial Assesment For Anion Exchange Membranes, Mohammad Pabel Kabir

Theses and Dissertations

In this thesis, cobaltocenium containing monomers and polymers are investigated for guest anion loading and alkaline anion exchange membrane for fuel cells. The properties of these polymers are characterized and discussed.

Chapter 1 describes overall background and potential applications of metallocene and metallocenium containing polymers. The research objectives of metallocenium containing polymers are discussed.

Cobaltocenium containing polymers have potential applications in biomedical science. Therefore, the effect of different parameters such as electrostatic charge, size, conformation, π-electron system and hydrogen bonding on the interactions between these polymers and small anionic probes has been studied qualitatively, as described in Chapter 2. A …

Bayesian Ensemble Of Regression Trees For Multinomial Probit And Quantile Regression, Bereket P. Kindo

Theses and Dissertations

This dissertation proposes multinomial probit Bayesian additive regression trees (MPBART), ordered multiclass Bayesian additive classification trees (O-MBACT) and Bayesian quantile additive regression trees (BayesQArt) as extensions of BART - Bayesian additive regression trees for tackling multinomial choice, multiclass classification, ordinal regression and quantile regression problems. The proposed models exhibit very good predictive performances. In particular, ranking among the top performing procedures when non-linear relationships exist between the response and the predictors. The proposed procedures can readily be applied on data sets with the number of predictors larger than the number of observations.

MPBART is sufficiently flexible to allow inclusion of …

The Influence Of Prenatal And Early Life Factors On Bmi Z Scores And The Risk Of Being Obese In Early Childhood, Junxiu Liu

Theses and Dissertations

Childhood obesity is a serious public health challenge. The underlying causes behind the rising levels of childhood obesity might be driven by prenatal and early life factors. Recently, several studies have examined the association between gestational weight gain (GWG) and the risk of obesity in the pediatric population. Findings on the association of both inadequate and excessive GWG with offspring obesity are inconsistent and vary by maternal prepregnancy body mass index (BMI) status. Inconsistent findings also exist for the association of GWG and BMIZ as a continuous outcome. Nevertheless, existing studies mainly focus on the BMIZ upper centiles using logistic …

The Effects Of Exercise On Sleep Parameters Among Older Women, Charity B. Breneman

Theses and Dissertations

Sleep is a modifiable behavior that commonly fluctuates from night to night within the same individual and varies across the lifespan. Over the years, sleep research has identified physical activity (PA)/exercise as a non-pharmacological approach to improving sleep. The overall goal of this dissertation was to investigate the acute effect of exercise on sleep outcomes as well as examine the relationship between night-to-night fluctuations in sleep parameters with exercise training among healthy older women.

Two studies, both utilizing a longitudinal study design, were conducted to 1) understand how acute exercise affects both behavioral and physiological sleep outcomes during the corresponding …

Finding Sanctuary In Sisterhood: A Middle School Literacy Group Critically Analyzes Race, Gender, And Size, Dywanna E. Smith

Theses and Dissertations

This critical qualitative study documented intersectionalities of race, gender, body image, and literacies as interpreted by five eighth grade Black female students during an eight-week after school literacy group. Ethnopoetic methodologies were used to represent findings and provide insights about how critical literacy engagements can be used to create discursive spaces where young Black girls describe and define these intersectionalities, how they are socialized into current beliefs, make meaning of beauty ideals portrayed in media, and interpret gender, obesity, and race. Through content analysis of field notes, audio recorded sessions, research partner interviews, researcher journals, and genre artifacts, this study …

Empowering The Enemy: The Cold War And The East-West Battle For Information Superiority, 1945 - 1969, Stan Trembach

Theses and Dissertations

This comparative study employs a historical perspective to narrow the gap in the existing knowledge of the origins of an information explosion phenomenon that dates back to the early decades of the twentieth century. It argues that the root cause of the unprecedented growth of the overall amount of documents was the rapid expansion of scientific and technical advances across the world and the subsequent spread of modern technologies, particularly those applied to scientific and technical information (STI).

This research is based on the premise that the thriving of the Soviet militaryindustrial complex went hand in hand with the rise …

The Effect Of Economic Inequality On Perceptions Of Merit, Nicholas Heiserman

Theses and Dissertations

Previous research has shown that the rewards people receive are often taken as indirect evidence of their competence. Meanwhile, economic inequality has increased in the US over the past several generations. I propose that variation in economic inequality – the distribution of rewards in society – alters perceptions of the merits of people at different strata in society according to an assumption of equity. I use Amazon’s Mechanical Turk (mTurk) to experimentally manipulate the level of inequality (high vs low) participants perceive in an anonymized country, and I measure participants’ perceptions of merit for people in that country’s 90th and …

Decadal Salinity Changes In The Oceanic Subtropical Gyres And Connection To Changes In The Global Water Cycle, Bryce Andrew Melzer

Theses and Dissertations

There is evidence that the global water cycle has been undergoing an intensification over several decades as a response to increasing atmospheric temperatures, particularly in regions with skewed evaporation – precipitation (E-P) patterns such as the oceanic subtropical gyres. However, observational data (rain gauges, etc.) can be quite sparse over such areas due to the inaccessibility of open ocean regions. This study utilizes in situ data, reanalysis, and model outputs to infer interannual to decadal scale trends in surface freshwater forcing within remote, evaporation-dominated subtropical regions of the ocean as they pertain to the past and present state of the …

Deciphering The Interacting Regions And Functions Of The Rad51d-Nono Protein Complex During Dna Damage Response, Latarsha Porcher

Theses and Dissertations

Genomic instability is one of the major components represented in the “Hallmarks of Cancer.” DNA interstrand crosslinks and double-stranded breaks are two of the most severe causes of genomic instability. Homologous recombination (HR) plays a major role in resolving both types of DNA lesions, requiring a homologous template and the RAD51 paralog complex of proteins. One member of this complex is the RAD51D ovarian cancer susceptibility gene product. To better elucidate RAD51D modifications and functions, several protein interaction screens were performed, and one of the novel proteins identified was NONO (a.k.a. p54nrb). Decreased expression of either RAD51D or NONO conferred …

The Role Of Nuclear Factor-Erythroid-2-Related Factor 2 In Sensitivity To Thymidylate Synthase Inhibitors In Colon Cancer Cells, Sarah Ashley Clinton

Theses and Dissertations

Nuclear factor-erythroid-2-related factor 2 (NRF2), a member of the cap ‘n’ collar family of bZIP transcription factors, confers protection against oxidative and electrophilic stress. NRF2 is of great interest in cancer research, due to its role in response to chemotherapy. The class of drugs targeting thymidylate synthase (TYMS) has been useful in the treatment of colorectal cancer, among other cancers. It has long been known that inhibition of TYMS leads to depletion of thymidine levels and the onset of programmed cell death, deriving from the enzyme’s function as the sole de novo source of thymidine for DNA replication and repair. …

Renewable Bio-Based Intermediates, Monomers And Polymeric Materials From Plant Oils, Liang Yuan

Theses and Dissertations

In the dissertation work, new chemical intermediates, monomers and thermoplastic polymers were derived from renewable soybean oil. Elastomers were further developed using monomers and polymers from soybean oil. The properties of these polymeric materials were characterized and discussed.

In Chapter 1, the overall background and recent development polymers from renewable biomass, especially plant oils, is introduced. Major research objectives of my doctoral research were described.

The first section of the dissertation, on the preparation of renewable intermediates, novel monomers and homopolymers, is provided in Chapter 2 and Chapter 3. In Chapter 2, amidation of soybean oil with amino alcohols was …