Digital Commons Network^™

Pengembangan Buku Ajar Matakuliah Mikrobiologi Dasar, Mohammad Wildan Habibi, Endang Suarsini, Mohammad Amin

Jurnal Pendidikan: Teori, Penelitian, dan Pengembangan

Microbiology is one of the subjects taken by the students of Biology Education graduate level. The learning process is often used for this just use the presentation and discussion, so that the student obtained just a concept. The purpose of this study is to make products in the form of research-based textbook current that can later be applied to the student. The method used is R & D (Research and Development) ADDIE. The results of this study is the product of the research-based textbook latest that has been validated by subject matter experts (91,25%), instructional media experts (92,85%), and user …

Origami Terhadap Kecerdasan Spasial Matematika Siswa, Dian Wardhani, Edy Bambang Irawan, Cholis Sa’Dijah

Jurnal Pendidikan: Teori, Penelitian, dan Pengembangan

Origami is an art of folding that originated in Japan. Origami is not only fun, but to accommodate the diversity of learning styles helps children understand math and this is an innovative method for the development of education, culture, and social skills. Origami also encourages interaction and train students in the group student collaboration. The use of origami media can also help the learning process of students in mathematics due to make the students involved in the learning process. Origami to create a form. Forms related to spatial intelligence, spatial intelligence thinking skills while using the pictures and imagine the …

The Construct Definition Of An English Language Teachers’ Content Knowledge, Reza Khani, Anna Hajizadeh

The Qualitative Report

Regardless of the increasing emphasis on the importance of language teachers’ content knowledge, there is a lack of consensus regarding its constituents. To this end, a panel of experts and non-experts were requested to express their opinions regarding the constructs of content knowledge (CK) in the format of an open-ended written questionnaire. The findings indicate that teachers’ CK includes eleven categories: teaching skills, structures, method and approaches, curriculum, language acquisition theories, classroom context, language testing theories, learner related knowledge, ELT research methods, and teacher related knowledge. The findings can help training educated teachers to be familiar with various aspects of …

Medical School Watercooler Newsletter - May 29, 2016, Lindsey Lyle

Watercooler Newsletter

This is the May 29, 2016 edition of the Frederick P. Whiddon College of Medicine's newsletter - Watercooler.

Contents Include:

• Dr. Trimm Named President of APPD
• Hippocrates Park Dedication Set for June 11
• USA Surgery Clinic Recognized for Customer Service
• USA Gastroenterology Welcomes Tanya Rowell
• Former USA Researcher Joins Harrison School of Pharmacy

Discovery Of Novel Biomarkers For Alzheimer's Disease From Blood, Jintao Long, Genhua Pan, Emmanuel Ifeachor, Robert Belshaw, Xinzhong Li

School of Engineering, Computing and Mathematics

Blood-based biomarkers for Alzheimer’s disease would be very valuable because blood is a more accessible biofluid and is suitable for repeated sampling. However, currently there are no robust and reliable blood-based biomarkers for practical diagnosis. In this study we used a knowledge-based protein feature pool and two novel support vector machine embedded feature selection methods to find panels consisting of two and three biomarkers. We validated these biomarker sets using another serum cohort and an RNA profile cohort from the brain. Our panels included the proteins ECH1, NHLRC2, HOXB7, FN1, ERBB2, and SLC6A13 and demonstrated promising sensitivity (>87%), specificity …

Franck Symphony In D Minor, Lawrence University Symphony Orchestra, May 28, 2016, Lawrence University

Conservatory of Music Concert Programs

Memorial Chapel

2016 Rose-Hulman Institute Of Technology : One-Hundred And Thirty-Eighth Commencement, Rose-Hulman

Commencement Programs

Severity And Clustering Of Menopausal Symptoms Among Obese And Nonobese Postmenopausal Women In India, E. S. Sharanya Shre, Kate Trout, Sonia Pant Singh, Awnish Kumar Singh, Surapaneni Krishna Mohan, Ashish Joshi

Publications and Research

Background: The symptoms of menopause have a negative impact on quality of life, especially in women transitioning to menopause and earlier transitions. This study was conducted with the objective of assessing the effect of obesity on the severity of menopausal symptoms and the clustering of symptoms in postmenopausal women in India.

Methodology: The Menopausal Rating Scale (MRS) was used to assess the severity of menopausal symptoms of postmenopausal women of Chennai, visiting Saveetha Medical College, Chennai, India. This cross‑sectional study was conducted from August to November 2013 in Chennai, India. Sociodemographic characteristics, anthropometric measurements, blood pressure level, menopausal history, personal …

May 28, 2016 (Pages 2651-2846), Pennsylvania Legislative Reference Bureau

Volume 46 (2016)

No abstract provided.

Oleanolic Acid Inhibits High Salt-Induced Exaggeration Of Warburg-Like Metabolism In Breast Cancer Cells, Suneetha Amara, Mu Zheng, Venkataswarup Tiriveedhi

Biology Faculty Research

Cancer cells have a proliferative advantage by utilizing intermediates of aerobic glycolysis (Warburg effect) for their macromolecule synthesis. Although the exact causes of this Warburg effect are unclear, high osmotic stress in solid tumor microenvironment is considered one of the important factors. Oleanolic acid (OA) is known to exert anti-inflammatory and anti-cancer effect. In our current studies, using breast cancer cell lines, we determined the protective role of OA in high salt-mediated osmotic stress-induced cancer growth. Hypertonic (0.16 M NaCl) culture conditions enhanced the cancer cell growth (26 %, p < 0.05) and aerobic glycolysis as marked by increased glucose consumption (34 %, p < 0.05) and lactate production (25 %, p < 0.05) over untreated cells. This effect was associated with increased expression and activity of key rate-limiting enzymes of aerobic glycolysis, namely hexokinase, pyruvate kinase type M2, and lactate dehydrogenase A. Interestingly, this high salt-mediated enhanced expression of aerobic glycolytic enzymes was efficiently reversed by OA along with the decreased cancer cell proliferation. In cancer cells, enhanced aerobic glycolysis is associated with the decreased mitochondrial activity and mitochondrial-associated caspase activity. As expected, high salt further inhibited the mitochondrial related cytochrome oxidase and caspase-3 activity. However, OA efficiently reversed the high salt-mediated inhibition of cytochrome oxidase, caspase activity, and pro-apoptotic Bax expression, thus suggesting that OA induced mitochondrial activity and enhanced apoptosis. Taken together, our data indicate that OA efficiently reverses the enhanced Warburg-like metabolism induced by high salt-mediated osmotic stress along with potential application of OA in anti-cancer therapy.

Peogress On Regulation Of Carotenoid Biosythesis By External Factorss, Yin Lin, Lin Junfang, Ye Zhiwei, Guo Liqiong, Jian Jinhui

Food and Machinery

In recent years, the carotenoids in nature are widely used in food, medicine, nutrition and health care industries due to the good biological activity and physiological function. And in order to meet the needs of human beings, the more and more studies on the regulation of carotenoid biosynthesis are emerging. In this paper, the effects of light, temperature and elicitor on the biosynthesis of carotenoids are reviewed, which is hoped that the production of carotenoids can be improved by external regulation in the production process.

Progress On Effects Of Different Additives On Gelatin Modification, Kang Shasha, Xin Ying, Chen Fusheng

Food and Machinery

Gelatin, the hydrolyzed biopolymer of collagen, is widely used in pharmaceutical, food industries etc. However, its inherent physical and chemical properties can not meet production needs (especially fish gelatin). The research of gelatin modification attracts more attentions. Gelatin chemical crosslinking modified with inorganic compounds (MgSO₄, CaCl₂ and NaCl), organic compounds (pectin, chitosan and aldehyde) and Transglutaminase was introduced in this paper.

Oleanolic Acid Inhibits High Salt-Induced Exaggeration Of Warburg-Like Metabolism In Breast Cancer Cells, Suneetha Amara, Mu Zheng, Venkataswarup Tiriveedhi

Chemistry Faculty Research

Cancer cells have a proliferative advantage by utilizing intermediates of aerobic glycolysis (Warburg effect) for their macromolecule synthesis. Although the exact causes of this Warburg effect are unclear, high osmotic stress in solid tumor microenvironment is considered one of the important factors. Oleanolic acid (OA) is known to exert anti-inflammatory and anti-cancer effect. In our current studies, using breast cancer cell lines, we determined the protective role of OA in high salt-mediated osmotic stress-induced cancer growth. Hypertonic (0.16 M NaCl) culture conditions enhanced the cancer cell growth (26 %, p < 0.05) and aerobic glycolysis as marked by increased glucose consumption (34 %, p < 0.05) and lactate production (25 %, p < 0.05) over untreated cells. This effect was associated with increased expression and activity of key rate-limiting enzymes of aerobic glycolysis, namely hexokinase, pyruvate kinase type M2, and lactate dehydrogenase A. Interestingly, this high salt-mediated enhanced expression of aerobic glycolytic enzymes was efficiently reversed by OA along with the decreased cancer cell proliferation. In cancer cells, enhanced aerobic glycolysis is associated with the decreased mitochondrial activity and mitochondrial-associated caspase activity. As expected, high salt further inhibited the mitochondrial related cytochrome oxidase and caspase-3 activity. However, OA efficiently reversed the high salt-mediated inhibition of cytochrome oxidase, caspase activity, and pro-apoptotic Bax expression, thus suggesting that OA induced mitochondrial activity and enhanced apoptosis. Taken together, our data indicate that OA efficiently reverses the enhanced Warburg-like metabolism induced by high salt-mediated osmotic stress along with potential application of OA in anti-cancer therapy.

A Discrit Narrative Case Study: How Are The Cards Stacked In Alternative School For African American Students With Disabilities?, Cherry Stanard

Doctor of Education in Special Education Dissertations

The purpose of this qualitative narrative case study is to develop a better understanding of how African American students with disabilities in alternative schools make sense of their educational journeys. Critical race theory and disability studies (DisCrit) is used as a framework for the investigation of (a) the process of being identified as a student with disabilities and (b) behavioral factors associated with placement in the alternative school environment, and (c) how students develop characteristics of resiliency in the alternative school environment. Five African American students with disabilities who were currently attending or who had previously attended alternative school were …

The Importance Of Nerve Microenvironment For Schwannoma Development, Alexander Schulz, Robert Büttner, Christian Hagel, Stephan L. Baader, Lan Kluwe, Johannes Salamon, Victor Felix Mautner, Thomas Mindos, David B. Parkinson, Jeffrey R. Gehlhausen, D. Wade Clapp, Helen Morrison

Peninsula Medical School

Schwannomas are predominantly benign nerve sheath neoplasms caused by Nf2 gene inactivation. Presently, treatment options are mainly limited to surgical tumor resection due to the lack of effective pharmacological drugs. Although the mechanistic understanding of Nf2 gene function has advanced, it has so far been primarily restricted to Schwann cell-intrinsic events. Extracellular cues determining Schwann cell behavior with regard to schwannoma development remain unknown. Here we show pro-tumourigenic microenvironmental effects on Schwann cells where an altered axonal microenvironment in cooperation with injury signals contribute to a persistent regenerative Schwann cell response promoting schwannoma development. Specifically in genetically engineered mice following …

Physicochemical Properties And Molecular Structure Characterization Of Digestion-Resistant Components In Enzymatic Branch-Chain Elongated Waxy Corn Starch, He Jian, Wang Ren, Zhang Hao, Zhou Xing, Chen Zhengxing

Food and Machinery

The resistant starch (RS) in enzymatic branch-chain elongated waxy corn starch (WCS) was separated by using in vitro digestion method. Then, the physicochemical properties and molecular structure of RS were characterized. The results showed that the morphology of starch was significantly changed after digestion, and irregular cracks appeared on the surface of RS. Moreover, the improvement of thermal stabilities of RS wasalso observed. These results revealed that the amorphous regions of modified starches were preferably to be hydrolyzed during digestion. Compared to modified WCS, the molecular weight of RS was profoundly decreased (6.03×10⁶～1.13×10⁸ g/mol). In addition, the …

Study On Oxidation Degradation Products Of Linoleic Acid, Wang Jianhui, Wang Xiu, Chen Qi, Wang Faxiang, Li Xianghong, Yu Jian, Liu Yongle

Food and Machinery

GC—MS was applied to identify the acceleration of oxidative products formed from linoleic acid stored at 60 ℃. Both volatile and non-volatile oxidates were focus on in the present study. The results showed that the oxidative products formed in the primary oxidation stage were continuously oxidized into volatiles (such as aldehydes, ketones, alcohols, furan, etc.) during the course of accelerated oxidation. The relative contents of Hexanal, (E)-2-Heptena, (E)-2-Nonena, (E)-3-Nonene-2-one, 2-pentyl Furan changed obviously before 12 h and they could be used as evaluation indices of the primary autoxidation of linoleic acid. However, the relative contents of (E, E)-2, 4-Nonadienal, (E, …

Preparation And Structure Characterization Of Whitmania Pigra Polypeptide-Zinc Chelates, Zeng Qingrui, Qian Wenju, Wang Mengfan, Zhou Yu, Chen Yuanyuan, Han Yaoping

Food and Machinery

Peptides were prepared form enzymatic hydrolysis of whitmania pigra protein by composite enzyme(papain and flavorzyme) and then made to react with zinc sulfate heptahydrate under certain conditions to produce polypeptide-zinc chelates that qualitative analysis by sodium sulfide method . The structure of polypeptide-zinc chelates was preliminary research by UV, IR, Fluorescence, X diffraction analysis technology to polypeptide and polypeptide-zinc chelates. The results show that the pH of the reaction system,the mass ratio of polypeptide and zinc,the concentration of whitmania pigra polypeptide have a great influence on the reaction of whitmania pigra peptides and zinc sulfate heptahydrate; By comparing analysis the …

Hybrid Neural Network Based On Software Measurement For Dm423 Biomass During Batch Cultivation, Zeng Xiangyan, Zhao Liangzhong, Li Bing, Li Lin

Food and Machinery

The biomass concentration estimated by the static feedforward multiplayer neural network of 5-5-1 topology and hybrid neural network - microbe growth model with five inputs of culture time, temperature, pH and dissolved oxygen and glucose concentration. The result showed that the static feedforward multiplayer neural network mean squared error (MSE) of testing samples 1.73×10^-3. And hybrid neural network - microbe growth model offered a much better generalization accuracy than that of single neural network model, with MSE of testing samples of 0.25×10^-3, it was found that there was some deviation between estimated biomass and actual values …

Research On Qualitative Analysis Of Vinegar By Using Near-Infrared Spectroscopy Combined With Som And Svm, Hao Yong, Zhao Xiang, Wen Qinhua, Chen Bin

Food and Machinery

Near-infrared spectroscopy (NIRS) combined with two discriminative analysis methods including self-organization mapping (SOM) and support vector machine (SVM) were used for discriminant analysis of vinegar with different production year and brand. Continuous wavelet transform (CWT) was adopted for spectra preprocessing. Principal component analysis (PCA) was used for spectra dimension reduction and space distribution analysis. The results shown that CWT can effective eliminate spectra translation error. PCA can greatly reduce characteristic spectrum variables and improve modeling efficiency. For identification of vinegar with different production year, the CWT-PCA-SOM method can get 97.37% correct recognition rate (CRR), and the CWT-PCA-SVM method can get …

Optimization On Hplc Detection And Pre-Treatment Conditions Of Patulin In Fresh Apple, Sun Lihua, Qian Huiping, Li Zhiwen, Chen Jiluan, Pang Xuequn, Yan Shijie

Food and Machinery

A rapid and sensitive method was established for the accurate determination of patulin in fresh apples. Experimental parameters including extraction methods, mobile phase composition and flow rate for HPLC which always affect the sensitivity and analysis efficiency of the method were optimized systemically. The results indicated that solid phase extraction (SPE) techniques using functionalized polystyrene-divinylbenzene column (PEP) exhibited satisfactory recoveries comparing with other extraction methods. Under the optimized chromatographic conditions, patulin could be well separated from other impurities within 4 min, and detected with a wide linear range between 10-25 000 μg/L and a detection limit of 10 μg/L (S/N=3) …

Study On Enhancing Antibacteial Activity Of Lactobacillus Plantarum Fmb10 Through Composite Mutagenesis, Chen Yunyun, Lu Zhaoxin, Dang Lijuan, Gao Yuqi

Food and Machinery

Induced mutation of Lactobacillus plantarum fmb10 divided from pineapple was performed by Co⁶⁰γ, low-energy N⁺ beam irradiation, DES, EMS and composite mutagenesis, respectively, to enhance the antibacterial activity. The results showed that Lactobacillus plantarum fmb10 was sensitive to low-energy N+ beam and EMS irradiation, and a high-activity strain fmb10-Z with stable inheritance and forward mutation was obtained after the treatment of EMS-low-energy N⁺ beam composite mutagenesis. The bacteriostatic diameter against E.coli was increased by 23%, and the antibacterial activity of the mutant fmb10-Z was increased 1.5 times compared with original strain.

Nondestructive Detection Of Internal Quality Of Loquat By Near Infrared Spectroscopy, Wang Xi, Wu Xiyu, Pang Lan, Xu Dan

Food and Machinery

Spectra data of the 101 loquat samples were obtained by using NIRS and the TSS, titratable acid, Vc of the samples were determined by conventional chemical methods. The mathematical pretreatment methods were used as spectral preprocessing options, and then the calibration models of TSS, titratable acid and Vc were established respectively by PLS. By using these models, the TSS, titratable acid and Vc in the validation sets can be predicted. The Rp2 are 0.906, 0.745, 0.554, RMSEP are 0.628, 0.048, 2.23 respectively, and the RPD of TSS is 3.31. Thus, the calibration model of TSS can be used for the …

Design And Structure Optimization Of The Multi-Spots Starter-Pressing Machine Of Daqu In Chinese Spirits, Xie Liangliang, Tian Jianping, Yang Haili, Zhang Liangdong, Meng Sidong

Food and Machinery

Considering the paste and wine can not meet the effect of the artificial bending due to the single structure and poor controllability of the existing Daqu liquor knee-piece bending press machine，which result in the discordance of the Daqu overall tightness. A new multiple-points flexible controlled bending press machine was designed. A multitude of hammers in different combinations repeatedly and multiple-points with flexibility crowed and sheared the block, which simulated the artificial start-pressing and block-making, so that better effect of the block with moderateness and uniformity could be achieved. The key component of which supporting plate structure was optimized and analyzed …

Determination On Freshness Of Strawberry Based On Electronic Nose And Ethanol Sensor, Xu Jing, Zhao Xiujie, Sun Ke, Wang Zhuo, Tu Kang

Food and Machinery

“Hongyan” strawberry was selected as test materials, with the storage temperature of 4 ℃, humidity of 85%~95%, to detect the strawberry freshness based on flavor. Electronic nose (e-nose) and the single ethanol sensor were used to collect the odor of strawberry during storage period. The strawberry was divided into four stages based on the changes of sensory evaluation score and Principal component analysis. By using the partial least squares discriminant analysis (PLS-DA) and classification support vector machine(SVM-C), the model of fresh degree of strawberry was established based on odor discrimination. The overall accuracy for modeling group and validation group were …

Preservative Effect Of Active Films Containing Pomegranate Peel Extract As Antimicrobial Agent On Chilled Pork, Hu Shuaifeng, Xie Qingchao, Liang Xianbo, Li Li

Food and Machinery

With 1.5% of pomegranate peel extract (PPE) as antimicrobial agent, polyethylene resin (PE) and polypropylene resin (PP) as base material, PE, PPE—PE, PP/PPE—PE active films were prepared and their basic properties were investigated. Pork stored at refrigeration (4±1) ℃ with PE, PPE—PE, PP/PPE—PE active packaging films, and the effects of preservation were evaluated by total viable count (TVC), total volatile basic nitrogen (TVB-N), pH value, thiobarbituric acid (TBA) value, drip loss and sensory scores. PPE—PE film was 1.96 times the oxygen transmission rate of PE film, 4 times of PP/PPE—PE film. PPE caused the light transmittance of PE film decreased, …

Effect Of Different Packaging Conditions On Storage Quality Of Akesu Walnut, Wang Ping, Song Lijun, Lu Jiankang, Hou Xujie

Food and Machinery

In order to find the best way of physical preservation and storage conditions of xin 2 in Akesu, using aluminum foil packaging, Inflatable packaging, vacuum packaging to wrap the walnuts were stored in low temperature (4 ℃) and room temperature (25 ℃), and controlled trial. Determining the acid value, koettstorfer numbe, iodine number, glucosinolates barbituric acid value, the peroxide value, the activity of lipase, peroxidase, catalase activity of oil from walnut, which change made as oxidation quality evaluation index, to study the difference of the changes of the indexes of different storage conditions using variance analysis. The results showed that: …

Response Surface Methodology For Optimization Of Extracting Polysaccharide From Polygala Tenuifolia Willd. And Antioxidant Evaluation, Jing Yongshuai, Wu Lanfang, Wang Qian, Zheng Yuguang, Yan Yuping, Jiang Aimin

Food and Machinery

The optimum condition of ultrasonic extraction of Polygala tenuifolia Willd. Polysaccharide (PTP) was investigated. The results showed, the optimum condition was ultrasonic extraction time 30 min, ultrasonic extraction, temperature 67 ℃, ratio of water to raw material 10∶1（mL/g）, and extraction 2 times, the yield of polysaccharide was（5.87±0.21）% (n=3). IR spectra predicted PTP had typical characteristic absorption peaks which were neutral polysaccharide and free from urinic acid. In vitro antioxidant activities showed PTP had effects on DPPH radical and hydroxyl radical.The IC₅₀ of it against DPPH radical and hydroxyl radical was 0.83 mg/mL, 1.29 mg/mL.

Optimization On Freezing Concentration Of Aqueos Extracts From Anoectochilus Roxburghii Rhizome, Wang Wencheng, Chen Meiying, Chen Jinquan

Food and Machinery

The freezing concentration of aqueos extracts feom the residual rhizome of Anoectochilus roxburghii has been investigated. The loss rate caused by entrainment of ice crystals and the rate of the freezing concentration were selected as the evaluation indexes to design the optimization of processing by the response surface methodology. The experimental results showed that the most ideal process were as the followed: the temperature difference between refrigerant and concentration 5 ℃, scraper speed 45 r/min, and freeze concentration time, 13.5 h. Under the conditions, the loss rate caused by entrainment o f ice crystals was 0.891 0% and the rate …

Research Progress In Immune Regulation Of Food-Derived Bioactive Peptides, Chen Yuehua, Cheng Yunhui, Xu Zhou, Wen Li

Food and Machinery

Food derived bioactive peptides are a series of specific functional hydrolysates of food proteins with relatively small molecular weight, containing deferent number of amino acid residues. And food-derived immunoactive peptides are those bioactive ones that play important roles on antibacterial, anti-inflammatory, and immunoregulation. Thus it is significant to investigate them because of their wide distribution and high bioactivities. The recent research status and the immune functions of immunopeptides are summarized. Moreover, some methods of producing immunoactive peptides were also introduced. Finally it is prospected that the research tendency of the immunoregulatory mechanism of immune peptides and their advanced utilization.