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Changes In The Levels Of Liver Hsp70, Plasma Nitric Oxide, And The Antioxidative System In An Experimentally Induced Endotoxemia Mouse Model And The Role Of Reduced Glutathione, Temel İlgün, Kezban Yildiz Dalginli, Canan Gülmez, Onur Ataki̇şi̇

Turkish Journal of Biology

Endotoxin molecules in lipopolysaccharides are among most important molecules that initiate a cascade of events in sepsis/endotoxemia. Lipopolysaccharide exposure may result in strong immune responses, disrupt the intracellular oxidant/antioxidant balance, and cause excessive reactive oxygen species generation. The purpose of the study was to examine if reduced glutathione (GSH) has a protective role against lipopolysaccharides. The effects of lipopolysaccharide (LPS) or GSH alone or in combination on the levels of the plasma antioxidant system, NO, and liver HSP70 were investigated. A total of 100 Swiss albino mice were divided into 4 groups as Group I (control), Group II (20 μg/kg …

A New Reliable And Sensitive Nested Pcr Assay Based On The Human Sry Gene For Detection Of Interspecific Chimeras, Jie Yu, Kaijing Li, Main Huang, Liping Guan, Min Zhang, Weihua Li, Jianfa Huang, Ting Luo, Wencong Wang, Bikun Xian, Xing Liu, Bing Huang

Turkish Journal of Biology

Although interspecific chimeras can be identified using laborious techniques, more accurate and rapid detectable methods need to be established. The present study develops a nested polymerase chain reaction (nPCR) assay to detect human-mouse chimeras. A set of previously validated outer primers, specific to the human SRY gene, was used for conventional one-step PCR, while the inner primers for nPCR were designed. The specificities of PCR and nPCR assays were examined; both primer sets yielded PCR amplification products from male human epidermis-derived mesenchymal stem cell-like pluripotent cell DNA but no amplification products from negative control DNA. The sensitivity of this nPCR …

Genotoxic Potential Of Cynanchum Sarcomedium Meve & Liede Coupled With Its Modulatory Action On Oxidative-Stress?Mediatedgenotoxicity By Hydrogen Peroxideneethu Kannan Bhagyanathan*, John Ernest Thoppilcell And Molecular Biology Laboratory, Department Of Botany, University Of Calicut, Kerala, India, Neethu Kannan Bhagyanathan, John Ernest Thoppil

Turkish Journal of Biology

The present study evaluated the genotoxic effect of aqueous extract of C. sarcomedium Meve and Liede and its protective role in chromosomal aberrations induced by 2% hydrogen peroxide (H2O2) on Allium cepa root meristem. Our results showed that the C. sarcomedium extract induced major chromosomal aberrations, viz., nuclear lesions, chromosome breaks, chromosome laggards, vagrance, stickiness, and bridges. These are irreversible in nature and indicate that the plant is a potent clastogen. Total chromosome aberrations were more frequent with increase in exposure time and concentrations of plant extract. For antigenotoxic screening, three different modes of treatment were used: pretreatment, posttreatment, and …

Cardiotoxic Effects Of Enrofloxacin On Electrophysiological Activity, Cardiac Markers, Oxidative Stress, And Haematological Findings In Rabbits, Ramazan Durgut, Oktay Hasan Öztürk, Ahmet Nacar, Ali̇ye Sağkan Öztürk

Turkish Journal of Zoology

The aim of this research was to investigate the effect of normal and high doses of enrofloxacin on basal electrocardiographic (ECG) parameters including corrected QT (QTc) values along with biochemical and haematological findings in healthy rabbits. In total 21 New Zealand rabbits were used. The animals were randomly divided into three groups including 7 rabbits in each. The normal dose (5 mg/kg) of enrofloxacin and a toxic dose (50 mg/kg) were given intravenously in group 1, and the normal dose was given for 6 weeks in group 2. The last group was designated as a negative control (group 3). ECG, …

The Road To Health: Schuylkill Health 2016 Community Health Needs Assessment, Lehigh Valley Health Network

LVHN Annual & Statistical Reports

No abstract provided.

Dengue Vaccine: The Need, The Challenges And Progress, Alan L. Rothman, Francis A. Ennis

Institute for Immunology and Informatics Faculty Publications

No abstract provided.

Cpg Improves Influenza Vaccine Efficacy In Young Adult But Not Aged Mice, Alejandro Ramirez, Mary Co, Anuja Matthew

Institute for Immunology and Informatics Faculty Publications

Several studies have shown a reduced efficacy of influenza vaccines in the elderly compared to young adults. In this study, we evaluated the immunogenicity and protective efficacy of a commercially available inactivated influenza vaccine (Fluzone^®) in young adult and aged mice. C57/BL6 mice were administered a single or double immunization of Fluzone^® with or without CpG and challenged intranasally with H1N1 A/California/09 virus. A double immunization of Fluzone^® adjuvanted with CpG elicited the highest level of protection in young adult mice which was associated with increases in influenza specific IgG, elevated HAI titres, reduced viral titres …

Tick‐, Mosquito‐, And Rodent‐Borne Parasite Sampling Designs For The National Ecological Observatory Network, Yuri P. Springer, Howard S. Ginsberg, Et Al

Plant Sciences and Entomology Faculty Publications

Parasites and pathogens are increasingly recognized as significant drivers of ecological and evolutionary change in natural ecosystems. Concurrently, transmission of infectious agents among human, livestock, and wildlife populations represents a growing threat to veterinary and human health. In light of these trends and the scarcity of long‐term time series data on infection rates among vectors and reservoirs, the National Ecological Observatory Network (NEON) will collect measurements and samples of a suite of tick‐, mosquito‐, and rodent‐borne parasites through a continental‐scale surveillance program. Here, we describe the sampling designs for these efforts, highlighting sampling priorities, field and analytical methods, and the …

Regulation Of Reactive Oxygen Species And The Antioxidant Protein Dj-1 In Mastocytosis, Do-Kyun Kim, Michael A. Beaven, Joseph M. Kulinski, Avanti Desai, Geethani Bandara, Yun Bai, Calman Prussin, Lawrence B. Schwartz, Hirsh Komarow, Dean D. Metcalfe, Ana Olivera

Internal Medicine Publications

Neoplastic accumulation of mast cells in systemic mastocytosis (SM) associates with activating mutations in the receptor tyrosine kinase KIT. Constitutive activation of tyrosine kinase oncogenes has been linked to imbalances in oxidant/antioxidant mechanisms in other myeloproliferative disorders. However, the impact of KIT mutations on the redox status in SM and the potential therapeutic implications are not well understood. Here, we examined the regulation of reactive oxygen species (ROS) and of the antioxidant protein DJ-1 (PARK-7), which increases with cancer progression and acts to lessen oxidative damage to malignant cells, in relationship with SM severity. ROS levels were increased in both …

Sex-Bias In Lupus-Prone (Nzbxnzw)F1 Mice: The Interplay Of Gr1+Cd11b+ Cells, Testosterone And Genetics, Abhishek Trigunaite

ETD Archive

Systemic Lupus Erythematosus (SLE) is a prototypic systemic autoimmune disorder predominantly (9:1) targeting females. Currently, there is no cure for SLE and also there is a lack of disease predicting biomarkers. The major aim of my research is to determine the cellular basis of the sex bias in SLE and subsequently facilitate the development of better therapeutics and biomarkers for the disease. In (NZBxNZW) F1 mice a similar female bias is observed as in humans and testosterone is shown to be protective against lupus-like disease development, but the mechanism is not well understood.

We found higher levels of Gr1hiCD11b+ myeloid …

The Pathogen-Occupied Vacuoles Of Anaplasma Phagocytophilum And Anaplasma Marginale Interact With The Endoplasmic Reticulum, Hilary K. Truchan, Chelsea L. Cockburn, Kathryn S. Hebert, Forgivemore Magunda, Susan M. Noh, Jason A. Carlyon

Microbiology and Immunology Publications

The genus Anaplasma consists of tick-transmitted obligate intracellular bacteria that invade white or red blood cells to cause debilitating and potentially fatal infections. A. phagocytophilum, a human and veterinary pathogen, infects neutrophils to cause granulocytic anaplasmosis. A. marginale invades bovine erythrocytes. Evidence suggests that both species may also infect endothelial cells in vivo. In mammalian and arthropod host cells, A. phagocytophilum and A. marginale reside in host cell derived pathogen-occupied vacuoles (POVs). While it was recently demonstrated that the A. phagocytophilum-occupied vacuole (ApV) intercepts membrane traffic from the trans-Golgi network, it is unclear if it or …

Involvement Of Nadh Oxidase In Biofilm Formation In Streptococcus Sanguinis, Xiuchun Ge, Xiaoli Shi, Limei Shi, Jinlin Liu, Victoria Stone, Fanxiang Kong, Todd Kitten, Ping Xu

Microbiology and Immunology Publications

Biofilms play important roles in microbial communities and are related to infectious diseases. Here, we report direct evidence that a bacterial nox gene encoding NADH oxidase is involved in biofilm formation. A dramatic reduction in biofilm formation was observed in a Streptococcus sanguinis nox mutant under anaerobic conditions without any decrease in growth. The membrane fluidity of the mutant bacterial cells was found to be decreased and the fatty acid composition altered, with increased palmitic acid and decreased stearic acid and vaccenic acid. Extracellular DNA of the mutant was reduced in abundance and bacterial competence was suppressed. Gene expression analysis …

Interaction Between Cannabinoid System And Toll-Like Receptors Controls Inflammation, Kathleen L. Mccoy

Microbiology and Immunology Publications

Since the discovery of the endocannabinoid system consisting of cannabinoid receptors, endogenous ligands, and biosynthetic and metabolizing enzymes, interest has been renewed in investigating the promise of cannabinoids as therapeutic agents. Abundant evidence indicates that cannabinoids modulate immune responses. An inflammatory response is triggered when innate immune cells receive a danger signal provided by pathogen- or damage-associated molecular patterns engaging pattern-recognition receptors. Toll-like receptor family members are prominent pattern-recognition receptors expressed on innate immune cells. Cannabinoids suppress Toll-like receptor-mediated inflammatory responses. However, the relationship between the endocannabinoid system and innate immune system may not be one-sided. Innate immune cells express …

Interaction Between Cannabinoid System And Toll-Like Receptors Controls Inflammation, Kathleen L. Mccoy

Microbiology and Immunology Publications

Since the discovery of the endocannabinoid system consisting of cannabinoid receptors, endogenous ligands, and biosynthetic and metabolizing enzymes, interest has been renewed in investigating the promise of cannabinoids as therapeutic agents. Abundant evidence indicates that cannabinoids modulate immune responses. An inflammatory response is triggered when innate immune cells receive a danger signal provided by pathogen- or damage-associated molecular patterns engaging pattern-recognition receptors. Toll-like receptor family members are prominent pattern-recognition receptors expressed on innate immune cells. Cannabinoids suppress Toll-like receptor-mediated inflammatory responses. However, the relationship between the endocannabinoid system and innate immune system may not be one-sided. Innate immune cells express …

Dendritic Cells Induce A Subpopulation Of Il-12rβ2-Expressing Treg That Specifically Consumes Il-12 To Control Th1 Responses, Uri Sela, Chaegyu Park, Andrew Park

Publications

Cytokines secreted from dendritic cells (DCs) play an important role in the regulation of T helper (Th) cell differentiation and activation into effector cells. Therefore, controlling cytokine secretion from DCs may potentially regulate Th differentiation/activation. DCs also induce de-novo generation of regulatory T cells (Treg) that modulate the immune response. In the current study we used the mixed leukocyte reaction (MLR) to investigate the effect of allospecific Treg on IL-12, TNFβ and IL-6 secretion by DCs. Treg cells were found to markedly down-regulate IL-12 secretion from DCs following stimulation with TLR7/8 agonist. This down-regulation of IL-12 was neither due to …

Student Comment: Not Really A Battle Of The Sexes: Women’S Health Agenda Advocates Global Equality In Medical Research Trials And Drug Administration, Margery R. Beltran

University of Baltimore Journal of International Law

The New Women’s Health Agenda seeks to close the discriminatory gap between men’s and women’s medical treatment around the world. Often, women’s reproductive issues are the focus of medical studies in which women are involved; however, chronic diseases are quickly becoming a high health risk for the female population around the world. This comment explores the past, present, and future of women’s global health. Throughout history, women have been prevented from participating in clinical trials for reproductive protection reasons. The problem arises after men have successfully responded to treatment because the medication is then administered to both men and women. …

Godey's, 1861, January-June, Vicki Betts

Godey’s Lady’s Book

Index to Godey's Lady's Book for January-June, 1861.

A Coprological Survey Of Golden Mantled Howler Monkeys (Alouatta Palliata Palliata) In The Osa Peninsula, Costa Rica, Jess M. Conatser

Theses, Dissertations and Capstones

Interactions between free-ranging primates and their associated parasitic forms continue to be a point of interest in ecology for several reasons. External and internal parasitism is not atypical for wild populations of primates, and the repercussions of these relationships can range from benign to life-threatening and capable of altering the structure of naturally occurring groups. The ecological relationship between the golden mantled howler monkey (Alouatta palliata palliata) and its host-specific ectoparasite, the howler monkey bot fly (Alouattamyia baeri) poses significant potential detriment to the overall body quality and success of the host primate. A. p. palliata has also been recorded …

Cancer Care Annual Report 2015-16, Children's Mercy Hospital

Cancer Center Annual Reports

Annual report of cancer care and oncology services at the Children's Mercy Hospital, Kansas City MO, a pediatric medical center. This report has a focus on neuroblastoma.

Rnai-Mediated Gene Knockdown By Microinjection In The Model Entomopathogenic Nematode Heterorhabditis Bacteriophora., Ramesh Ratnappan, Jonathan Vadnal, Melissa Keaney, Ioannis Eleftherianos, Damien O'Halloran, John M. Hawdon

Microbiology, Immunology, and Tropical Medicine Faculty Publications

BACKGROUND: Parasitic nematodes threaten the health of humans and livestock and cause a major financial and socioeconomic burden to modern society. Given the widespread distribution of diseases caused by parasitic nematodes there is an urgent need to develop tools that will elucidate the genetic complexity of host-parasite interactions. Heterorhabditis bacteriophora is a parasitic nematode that allows simultaneous monitoring of nematode infection processes and host immune function, and offers potential as a tractable model for parasitic nematode infections. However, molecular tools to investigate these processes are required prior to its widespread acceptance as a robust model organism. In this paper we …

A Single Center Observational Study On Emergency Department Clinician Non-Adherence To Clinical Practice Guidelines For Treatment Of Uncomplicated Urinary Tract Infections, Catherine Zatorski, Mark Zocchi, Sara Cosgrove, Cynthia Rand, Gillian Brooks, Larissa May

Emergency Medicine Faculty Publications

Background

The Emergency Department (ED) is a frequent site of antibiotic use; poor adherence with evidence-based guidelines and broad-spectrum antibiotic overuse is common. Our objective was to determine rates and predictors of inappropriate antimicrobial use in patients with uncomplicated urinary tract infections (UTI) compared to the 2010 International Clinical Practice Guidelines (ICPG).

Methods

A single center, prospective, observational study of patients with uncomplicated UTI presenting to an urban ED between September 2012 and February 2014 that examined ED physician adherence to ICPG when treating uncomplicated UTIs. Clinician-directed antibiotic treatment was compared to the ICPG using a standardized case definition for …

First Described Case Of Group B Streptococcus Pelvic Abscess In A Patient With No Medical Comorbidities, Paul Tyan, Elias Abi-Khalil, Karthik Dwarki, Gaby N. Moawad

Obstetrics and Gynecology Faculty Publications

Background. Group B Streptococcus is an organism that commonly infects a wide range of hosts including infants in the first week of life, pregnant women, and older age adults as well as adults with underlying medical comorbidities.

Case. Large pelvic abscess in a nonpregnant patient found to be caused by Group B Streptococcuswas treated successfully with IR guided drainage and antibiotics.

Conclusion. Though rare, GBS can still be a cause of invasive infection even in individuals who are nonpregnant and have no underlying comorbidities. Empiric antibiotic coverage for this organism should be kept in mind when treating an abscess.

"When Should I Tell?": Perspectives On Disclosure To Their Children Among Parents With Perinatally Acquired Hiv., Cynthia D Fair, Hannah Allen, Constance Trexler, Janet Osherow, Lawrence D'Angelo

Pediatrics Faculty Publications

Adolescents and young adults (AYA) with perinatally acquired HIV (PHIV) engage in developmentally expected behaviors, such as establishing relationships and having children. Previous research has focused on pregnancy management/outcomes of AYA with PHIV. However, little research has focused on the parenting experiences of this emerging cohort and on their views of disclosure to their offspring. This article examines data from a pilot study of five AYA parents with PHIV on disclosure to their child(ren) (n = 7, 6 HIV-negative). Disclosure of their own HIV status to their children is on the minds of parents with PHIV. However, few currently have …

Zika Virus, Abigail Hanneken

Nursing Student Class Projects (Formerly MSN)

Zika virus (ZIKV) is a member of the Spondweni serocomplex within the genus Flavivovirus, family flaviviridae (Weaver et. al, 2012). ZIKV is related to dengue, West Nile, and yellow fever viruses (Lazear et. al, 2016). ZIKV was first discovered in Uganda, but since then outbreaks have been reported in Africa and Southeast Asia. In May 2015, the Pan American Health Organization (PAHO) issued an alert regarding the first confirmed Zika virus infection in Brazil, where it began to rapidly spread throughout the region (CDC, 2016). Recently, the World Health Organization (WHO) declared the ongoing outbreak of Zika virus and associated …

Sepsis In The Intensive Care Setting, Katie Mojzisik

Nursing Student Class Projects (Formerly MSN)

Sepsis is a complex systemic illness. According to Kruse et al. (2016), neutropenic sepsis is a frequent complication in cancer patients. Although the underlying disease is curable, once transferred to the ICU with sepsis these patients have poor outcomes. • Patients who develop sepsis are commonly admitted to an Intensive Care Unit. Working on the Medical Intensive Care Unit (JMICU) at the James Cancer Hospital, one sees septic cancer patients often. This cancer diagnosis places patients at a higher risk of developing sepsis. Some patients also have a high risk due to decreased immunity from chemotherapy treatments. Oncology patients can …

Integrating Inflammasome Signaling In Sexually Transmitted Infections, Christopher Lupfer, Paras K. Anand

College of Natural and Applied Sciences

Inflammasomes are cytosolic multiprotein platforms with pivotal roles in infectious diseases. Activation of inflammasomes results in proinflammatory cytokine signaling and pyroptosis. Sexually transmitted infections (STIs) are a major health problem worldwide, yet few studies have probed the impact of inflammasome signaling during these infections. Due to the dearth of appropriate infection models, our current understanding of inflammasomes in STIs is mostly drawn from results obtained in vitro, from distant infection sites, or from related microbial strains that are not sexually transmitted. Understanding how inflammasomes influence the outcome of STIs may lead to the development of novel and effective strategies to …

Final Report: Storm Water Pollutant Inputs To The Little Miami River Via Yellow Springs And Birch Creeks, Audrey E. Mcgowin

Chemistry Faculty Publications

This report describes the results of research performed with funds from the Sture Fredrik Anliot Grant Fund granted in March 2015. The primary aim of the project was to identify and measure various stormwater pollutant inputs to Yellow Springs Creek and Birch Creek into Glen Helen Nature Preserve and then into the Little Miami River in Greene County, Ohio from Spring 2015 to Winter 2016.

In order to better understand pollutant flow into Glen Helen and subsequently into the Little Miami River, three different types of samples were taken. One type of sample was a “grab” sample taken under normal …

Sub-Saharan Africa’S “Other Diseases”: A Comparison Of The Global Response To Neglected Tropical Diseases And Hiv/Aids In Ghana, Rebecca Brosch

Political Science: Student Scholarship & Creative Works

Neglected tropical diseases (NTDs) refer to a group of diseases that affect 1.4 billion people worldwide, most commonly in low and low-middle income nations. These conditions can cause disabling and disfiguring effects that keep those inflicted from escaping poverty, leading to increased economic losses in already impoverished areas in Ghana and the rest of Sub-Saharan Africa. Although NTDs affect significantly more of the Ghanaian population, more government resources are given to HIV/AIDS (which only affects an estimated 1.5% of citizens). This research identifies the current response to NTDs (including government policy and NGO support) as well as how more global …

Exome Screening To Identify Loss-Of-Function Mutations In The Rhesus Macaque For Development Of Preclinical Models Of Human Disease, Adam Cornish, Robert M. Gibbs, Robert B. Norgren

Journal Articles: Genetics, Cell Biology & Anatomy

BACKGROUND: Exome sequencing has been utilized to identify genetic variants associated with disease in humans. Identification of loss-of-function mutations with exome sequencing in rhesus macaques (Macaca mulatta) could lead to valuable animal models of genetic disease. Attempts have been made to identify variants in rhesus macaques by aligning exome data against the rheMac2 draft genome. However, such efforts have been impaired due to the incompleteness and annotation errors associated with rheMac2. We wished to determine whether aligning exome reads against our new, improved rhesus genome, MacaM, could be used to identify high impact, loss-of-function mutations in rhesus macaques that would …

Community Acquired Pneumonia Project, Patricia Heaney

Doctor of Nursing Practice Projects

There is an increasing number of microorganisms that are becoming resistant to antimicrobial medications. This growing number of resistant organism poses a serious health threat, not only in this nation, but throughout the world. The Center for Disease Control and Prevention (CDC) estimates that there are greater than 2 million people who acquire serious infections that are resistant to one or more antimicrobial agents that were made to treat those organisms (CDC, 2013). Streptococcus pneumonia (S. pneumoniae or pneumococcus) is one such organism that has been identified by the CDC (2013) as becoming increasingly resistant to drugs that were once …