A Study On The Role And Regulation Of The Na+-Leak Channel, Non-Selective (Nalcn) In Myometrial Function, Erin Lynn Reinl

Arts & Sciences Graduate Student Theses and Dissertations

Uterine contractions are tightly regulated throughout pregnancy, remaining relatively quiescent for the majority of pregnancy to promote fetal development, while becoming highly activated at term to effectively expel the fetus. Contractions are coordinated through electrical and mechanical coupling of myometrial smooth muscle cells (MSMCs). Ion channel activity, which alters the electrical activity of MSMCs, can directly affect the contraction profile. Thus there is great interest in the field of reproduction to understand which ion channels regulate uterine excitability, and particularly how they may enable the transition from uterine quiescence to uterine activation for labor. The focus thus far has been …

Regulation Of Mycobacterial Transcription Initiation, Jayan Rammohan

Arts & Sciences Graduate Student Theses and Dissertations

Cellular life relies on gene expression, in which DNA is first transcribed into RNA, which is then translated into protein. Transcription is performed by the protein RNA polymerase (RNAP), which interacts with DNA in three sequential events known as initiation, elongation and termination. Mycobacterium tuberculosis (Mtb) represents a global burden to public health, and the transcription factors CarD and RbpA are both essential to Mtb. I have studied the effect of CarD and RbpA on transcription initiation in vitro at the Mtb rrnAP3 promoter. I have shown that CarD stabilizes unwinding of promoter DNA by RNAP using a two-tiered kinetic …

Mass Spectrometry And Protein Analysis: Structure, Oligomerization And Interaction, Hanliu Wang

Arts & Sciences Graduate Student Theses and Dissertations

Protein aggregation is common in biological processes including neurodegenerative diseases, as well as artificial development in biopharmaceutical production and protein storage. Therefore, understanding proteins involved in aggregation systems is important. It is generally difficult for high resolution structural methods, such as X-ray or NMR, to interrogate proteins possessing aggregation propensities. On the other hand, techniques that tolerate solution heterogeneities, such as circular dichroism, only provide global or low-resolution information. Mass spectrometry (MS)-based methods are appropriate and powerful in such applications because they can deal with mixtures. Additionally, primary sequence, post-translational modification, and structural information can be obtained at low sample …

Investigating The Life-Cycle Of Photosystem Ii Using Mass Spectrometry, Daniel Avram Weisz

Arts & Sciences Graduate Student Theses and Dissertations

Photosystem II (PSII) is a protein complex found embedded in the thylakoid membranes of all organisms that perform oxygenic photosynthesis. PSII converts sunlight into chemical energy, filling our atmosphere with molecular oxygen in the process and supporting nearly all life on Earth. PSII undergoes frequent light-induced damage as an unavoidable result of the electron transfer reactions it catalyzes. When damaged, PSII is disassembled, repaired, and reassembled in an intricate, tightly regulated process. The structure and mechanism of function of active PSII are relatively well-understood, due to the available crystal structures of the active complex and many years of biochemical and …

Discovery And Functional Implication Of Genetic Alterations Associated With Clonal Hematopoietic Expansion, Mingchao Xie

Arts & Sciences Graduate Student Theses and Dissertations

Cancers, including hematologic malignancies, arise as a result of the stepwise accumulation of mutations. Some early mutations that potentially initiate clonal expansion might exist in patients many years before they develop obvious disease symptoms. Therefore, identifying and characterizing these early mutations are critical to understanding the genetic basis of tumorigenesis. Here, we analyzed blood-derived DNA sequencing data from 2,728 individuals without apparent hematologic malignancies and identified 77 blood-specific mutations in 31 cancer-associated genes. Importantly, 83% of all mutations occurred in 19 genes that have been previously linked to hematological malignancies, such as DNMT3A, TET2, JAK2, and ASXL1. By investigating these …

Research Express News, Georgia Southern University

Research & Economic Development

• Georgia Southern faculty continue excellence in 2015

2016-December-15-Socon Faculty Staff Team, University Marketing And Commucications, East Tennessee State Univeristy

ETSU News

No abstract provided.

Selective Suppression Of The Α Isoform Of P38 Mapk Rescues Late-Stage Tau Pathology, Nicole Maphis, Shanya Jiang, Guixiang Xu, Olga N. Kokiko-Cochran, Saktimayee M. Roy, Linda J. Van Eldik, D. Martin Watterson, Bruce T. Lamb, Kiran Bhaskar

Sanders-Brown Center on Aging Faculty Publications

Background: Hyperphosphorylation and aggregation of tau protein are the pathological hallmarks of Alzheimer’s disease and related tauopathies. We previously demonstrated that the microglial activation induces tau hyperphosphorylation and cognitive impairment via activation of p38 mitogen-activated protein kinase (p38 MAPK) in the hTau mouse model of tauopathy that was deficient for microglial fractalkine receptor CX3CR1.

Method: We report an isoform-selective, brain-permeable, and orally bioavailable small molecule inhibitor of p38α MAPK (MW181) and its effects on tau phosphorylation in vitro and in hTau mice.

Results: First, pretreatment of mouse primary cortical neurons with MW181 completely blocked inflammation-induced p38α MAPK activation and AT8 …

Getting Road Expansion On The Right Track: A Framework For Smart Infrastructure Planning In The Mekong, Andrew Balmford, Huafeng Chen, Ben Phalan, Mingcheng Wang, Christine S. O'Connell, Cath Tayleur, Jianchu Xu

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

The current unprecedented expansion of infrastructure promises to enhance human wellbeing but risks causing substantial harm to natural ecosystems and the benefits they provide for people. A framework for systematically and proactively identifying the likely benefits and costs of such developments is badly needed. Here, we develop and test at the subregional scale a recently proposed global scheme for comparing the potential gains from new roads for food production with their likely impact on biodiversity and ecosystem services. Working in the Greater Mekong—an exceptionally biodiverse subregion undergoing rapid development—we combined maps of isolation from urban centres, yield gaps, and the …

Wsu Board Of Trustees Meeting Minutes, December 15-16, 2016

Board of Trustees Minutes

Minutes from the Wright State University Board of Trustees Meeting held on December 15-16, 2016.

Moral Distress, Leadership Integrity, Ethical Climate And Turnover Intent In Critical-Care Nurses, Desha L. Johnson Makiya

Nursing Theses and Dissertations

Objectives: To determine what relationships exist between moral distress, perceived leadership integrity, ethical climate, and turnover intent among adult critical care nurses and the moderating effect of leadership integrity and ethical climate on moral distress and subsequent turnover intent; to obtain a richer and fuller understanding of how critical care nurses experience moral distress within the context of critical care work.

Participants: Adult critical care nurse members of AACN (n=254), 22-70 years old, recruited February – March 2016.

Methods: A Qualtrics survey posted on the AACN eNewsline included the moral distress scale-revised, perceived leadership integrity scale, hospital ethical …

Plate Waste Trends Of 5​Th​-8​Th​ Grade Students At A Residential Environmental Learning Center, Colleen K. Foehrenbacher

School of Education and Leadership Student Capstone Theses and Dissertations

This descriptive research determined, using aggregated nonselective plate waste measurements, the characteristics of plate waste generated by 5th-8th grade students visiting Eagle Bluff Environmental Learning Center in Lanesboro, Minnesota over the course of 5 years. The results indicated a positive relationship between plate waste and group size; that groups from rural schools wasted less plate waste than schools from urban or suburban locations; and that groups from private schools wasted less than groups from public school groups. Completely eliminating plate waste is unrealistic, but reducing plate waste lowers user costs, makes program operations more efficient, and enhances a program’s success …

Developing An Educational Toolkit For Caregivers Of Six-To-Eighteen-Month-Olds Mixing Developmental Milestones And Outdoor Education, Jessica K. Hillstrom

School of Education and Leadership Student Capstone Theses and Dissertations

This capstone addressed the research question, Would the creation of an environmental, outdoor education toolkit help significantly benefit very young children reach their childhood milestone goals? It documents the creation of an educational toolkit for children aged 6 – 18 months old, which integrated age appropriate developmental milestone games and activities with outdoor themed activities. The goal was to prove that proving parents and caregivers with a toolkit for this age group of 6–18-month-old children would help make parents interested in spending more time outdoors, provide a cost-affective method for early outdoor exposure, and help parents save time by using …

Breaking Barriers: Saudi Women In Non Traditional Careers, Suad Dukhaykh

Student Scholarship

Women occupy only 15.8 percent of the private and public positions occupied by Saudi nationals, according to statistics from the Central Department of Statistics and Information 2015. The Saudi government has highlighted the need to support women's higher education and combat sociopolitical barriers to their employment. Despite facing substantial challenges to entering the labor market, females have made gains in Saudi society; these include higher literacy rates, greater employment, and moderate rates of entry into both positions of leadership and nontraditional careers. Qualitative research was based on semi-structured interviews with 30 women, 18 of whom had nontraditional careers and 12 …

A Preliminary Examination Of Elevated Blood Lead Levels In A Rural Georgia County, R. Christopher Rustin, Yu Sun, Chris Calhoun, Christy Kuriatnyk

Environmental Health Sciences: Faculty Publications

Background: Rural areas are often viewed as lower risk for lead poisoning and toxic exposures seriously impacting development of the brain and central nervous system; this report examines the prevalence of elevated blood lead levels for children <6 years of age in rural Ben Hill County, GA.

Methods: Lead surveillance data from the Georgia Department of Public Health (DPH) were analyzed using SAS®v-9.3 to calculate the prevalence of elevated blood lead levels (≥5ug/dL) among those children in Ben Hill County who had been tested for lead; the results were compared to Georgia and national data.

Results: A preliminary analysis of 2010-2015 screening data for Ben Hill County indicates that 8.73% …

An Assessment Of Data Related To Inspections Of Risk Factors For Public Swimming Pools, Shanita Shack, Maurice Redmond, R. Christopher Rustin

Environmental Health Sciences: Faculty Publications

Background: The Georgia Department of Public Health (DPH) is mandated to ensure that public swimming pools are safe for those who use them. This mandate is carried out by the DPH and local environmental health professionals through regulations and inspections. In 2015, legislation was introduced proposing to reduce the authority of the DPH to inspect certain pool types (apartments, subdivision, condominiums) and thus reduce regulatory protections in place for swimmers. To ensure that the DPH had current information on the risks associated with pools, the EH team, with assistance from a graduate student, analyzed inspection data to evaluate risk factors …

Alexander Arbuthnot And The Lyric In Post-Reformation Scotland, Joanna Martin

Studies in Scottish Literature

Presents the first critical discussion of manuscript poems in the Maitland Quarto attributable to Alexander Arbuthnot (1538-1583), the first Protestant principal of King's College, Aberdeen; gives detailed discussion of attribution and textual issues; and discusses the effects of religious change on Arbuthnot's writing of amatory, ethical and devotional lyric in post-Reformation Scotland.

From Shtetl To Stardom: Jews And Hollywood, Vincent Brook, Michael Renov

Purdue University Press Books

The outsized influence of Jews in American entertainment from the early days of Hollywood to the present has proved an endlessly fascinating and controversial topic, for Jews and non-Jews alike. From Shtetl to Stardom: Jews and Hollywood takes an exciting and innovative approach to this rich and complex material. Exploring the subject from a scholarly perspective as well as up close and personal, the book combines historical and theoretical analysis by leading academics in the field with inside information from prominent entertainment professionals. Essays range from Vincent Brook’s survey of the stubbornly persistent canard of Jewish industry “control” to Lawrence …

Harvesting A Sustainable Energy Future: Examining The Effect Of Chemical Composition On The Electromechanical Properties Of Polymer Gel Beads, Kaushik Kudtarkar

Theses

Clean energy is required to ensure global prosperity and economic growth. Increased industrialization is expected to increase energy demand by 50% by 2035. This will result in increased air pollution. Carbon dioxide emissions have been linked to global warming and other pollution related problems. These emissions can be reduced by recapturing the waste energy in the form of heat, vibration, and friction.

Common applications like transportation can lose as much as 78% of the energy they generate. Energy harvesters can be used to recapture energy lost through vibration, heat, etc. This recaptured energy will be reused and hence, we don’t …

A Proposed Imaging System To Spatially And Temporally Monitor Unburned Hydrocarbons In Jet Engine Exhaust, Emily E. Berkson

Theses

The local air quality surrounding airports is significantly impacted by ground-based airplane operations, such as landing, taxiing, idling, and takeoff. In recent years, studies performed across the country revealed that people living near major airports suffer from increased cancer risks and higher likelihoods of developing respiratory issues. Jet engine emissions have been monitored extensively by direct probing of the exhaust, but there are currently no inexpensive, portable, and unobtrusive methods with which to study exhaust plume dynamics. This study assesses the feasibility of designing a multispectral imaging system to track the spatial-temporal nature of a jet engine plume. Ideally, the …

Isolation Of Pharmaceutical-Degrading Bacteria From Lake Sediments Associated With Wastewater Effluents, Noreen Anne Gallagher

Theses

Many wastewater treatment plants (WWTPs) are not properly equipped for the removal of various compounds, including non-steroidal anti-inflammatory drugs (NSAIDs), analgesics, and hormones. These compounds are continually discharged into surface waters, which has become an emerging issue for environmental and public health. Microorganisms in the natural environment may play a crucial role in ecosystem self-purification processes such as contaminant degradation. The aim of this research was to determine if there were microorganisms from water and sediment samples located near wastewater effluent outfalls in Central and Western New York capable of degrading ibuprofen, naproxen, acetaminophen, and 17β-estradiol, and if the degradation …

Intersectionality Of Non-Normative Identities In The Cultures Of Engineering Survey, Allison Godwin, Adam Kirn, Lisa Benson, Geoff Potvin

School of Engineering Education Working Papers

This is a survey measuring students' attitudes and beliefs in engineering. The survey was distributed at four institutions across the U.S. in the fall semester of 2015. Students in first semester first-year engineering courses were surveyed on a paper-and-pencil instrument as part of a larger study. We collected 2,916 valid student responses that were digitized by the research group and audited for accuracy. Validity evidence for this survey has been document in ASEE conference proceedings.

From Shtetl To Stardom: Jews And Hollywood, Vincent Brook, Michael Renov

Purdue University Press Book Previews

The outsized influence of Jews in American entertainment from the early days of Hollywood to the present has proved an endlessly fascinating and controversial topic, for Jews and non-Jews alike. From Shtetl to Stardom: Jews and Hollywood takes an exciting and innovative approach to this rich and complex material. Exploring the subject from a scholarly perspective as well as up close and personal, the book combines historical and theoretical analysis by leading academics in the field with inside information from prominent entertainment professionals. Essays range from Vincent Brook’s survey of the stubbornly persistent canard of Jewish industry “control” to Lawrence …

Cost Effectiveness Of Potential Art Adherence Monitoring Interventions In Sub-Saharan Africa, Andrew N. Phillips, Valentina Cambiano, Fumiyo Nakagawa, Loveleen Bansi-Matharu, Papa Salif Sow, Peter Ehrenkranz, Deborah Ford, Owen Mugurungi, Tsitsi Apollo, Joseph Murungu, David R. Bangsberg, Paul Revill

OHSU-PSU School of Public Health Faculty Publications and Presentations

Background -Interventions based around objective measurement of adherence to antiretroviral drugs for HIV have potential to improve adherence and to enable differentiation of care such that clinical visits are reduced in those with high adherence. It would be useful to understand the approximate upper limit of cost that could be considered for such interventions of a given effectiveness in order to be cost effective. Such information can guide whether to implement an intervention in the light of a trial showing a certain effectiveness and cost.

Methods - An individual-based model, calibrated to Zimbabwe, which incorporates effects of adherence and resistance …

Physiological, Behavioral, And Knowledge Assessment Of Runners’ Readiness To Perform In The Heat, Yuri Hosokawa

Doctoral Dissertations

Many intrinsic and extrinsic factors may contribute to exertional heat stroke (EHS) susceptibility. Despite many plausible risk factors for EHS in runners, much remains unknown about how risk factors contribute to EHS risk and how education to modify behavior may reduce EHS risk. Therefore, this study investigated: (1) the differences between runners’ planned and actual hours of sleep, hydration strategies, and intake of medication and supplements at the 2016 Falmouth Road Race (FRR) and their current knowledge on heat safety and hydration, (2) effectiveness of educational video intervention in improving runner’s knowledge on heat safety and hydration, (3) effectiveness in …

The Role Of Prospective And Retrospective Cognition In Adolescent Mental Health, Gabriel F. Byer-Alcorace

Doctoral Dissertations

This study attempted to replicate the methods of Miles, MacLeod, and Pote (2004) who attempted to extend the application of a theory proposed by Andrew MacLeod and colleagues in the late 1990s. Only the aforementioned study has examined this theory with adolescents ages 14 to 19 year olds enrolled in public schools and 18 to 19 year olds enrolled universities. In the present study 169 students were asked to complete an assessment battery containing measures of depression, anxiety, stress, hope, and demographic information, as well as completing a positive and negative cognition task. It was hypothesized that a strong negative …

Development And Validation Of A Novel Bioassay To Determine Glucocorticoid Sensitivity, Emily L. Williams, Madeleine L. Stimpson, Peter L. Collins, Doyo G. Enki, Ashish Sinha, Richard W. Lee, Ashwin D. Dhanda

Peninsula Medical School

No abstract provided.

Evaluation Of The Nutritional Options For Wellness (Now) Program: Mitigating Cardiovascular Disease And Type 2 Diabetes Through Food Pantries, Sarah L. Henning

Masters Theses

Individuals with a lower socioeconomic status are at higher risk for chronic disease diagnosis and complications (Gerteis et al., 2014). The NOW Program is a social service program that was created in order to relieve some of the burden placed on these individuals utilizing the charitable food system. The purpose of the study was to perform a quantitative program evaluation on the NOW Program which is administered by pantry coordinating organization. The NOW Program connects chronically ill, low income members of Kent County with local food pantries that offer diet specific food and health education about their specific chronic disease …

Wage Shocks And The Technological Substitution Of Low-Wage Jobs, Daniel Aaronson, Brian Phelan

Upjohn Institute Working Papers

We extend the task-based empirical framework used in the job polarization literature to analyze the susceptibility of low-wage employment to technological substitution. We find that increases in the cost of low-wage labor, via minimum wage hikes, lead to relative employment declines at cognitively routine occupations but not manually-routine or non-routine low-wage occupations. This suggests that low-wage routine cognitive tasks are susceptible to technological substitution. While the short-run employment consequence of this reshuffling on individual workers is economically small, due to concurrent employment growth in other low-wage jobs, workers previously employed in cognitively routine jobs experience relative wage losses.

Electropolymerization Of B–Cyclodextrin Onto Multi–Walled Carbon Nanotube Composite Films For Enhanced Selective Detection Of Uric Acid, Mulugeta B. Wayu, Luke T. Dipasquale, Margaret A. Schwarzmann, Samuel D. Gillespie, Michael C. Leopold

Chemistry Faculty Publications

An amperometric uric acid (UA) sensor incorporating a multi-walled carbon nanotubes (MWCNT) network in Nafion and electropolymerized β-cyclodextrin (β-CD) layer is investigated. The electrochemical sensor is comprised of a glassy carbon electrode modified with Nafion-MWCNT nanocomposite film, a β-CD polymer inner selective layer, and a Hydrothane polyurethane (HPU) outer selective coating. The surface morphology and electronic structure of the electrode material are characterized using transmission electron microscopy (TEM), scanning electron microscope (SEM), and Fourier transform infrared (FTIR) spectroscopy. The electrocatalytic activity of the sensor is studied using cyclic voltammetry (CV), chronocoulometry (CC) and differential pulse voltammetry (DPV). Analytical performance of …