Digital Commons Network^™

The Impact Of Tickling Rats On Human-Animal Interactions And Rat Welfare, Megan Renee Lafollette

Open Access Theses

Rats initially fear humans which can lead to negative affect, poor welfare, and difficult handling. Also, modeling and measuring positive affect states in rats can pose an additional challenge. Heterospecific play, or “tickling,” is a handling habituation technique that mimics rat rough-and-tumble play that is being used to study positive affect. It can also be used to reduce fear of human, improve welfare, and elicit a positive affect state. However, current studies implementing the technique in laboratory rats use a wide variety of protocols to achieve differential results. Unlike in laboratory environment, pet store rats experience high levels of novelty …

Mathematical Analysis Of Feedback Targets Of Bmp Signaling In Drosophila Embryonic Development, Yan Luo

Open Access Theses

Bone morphogenetic proteins (BMPs) drive a range of cellular processes especially in the early stages of embryonic development. This family of proteins acts as one of the most important extracellular signals in development pattern formation across the animal kingdom. Cells in embryos differentiate into different cell types in response to the concentration level of BMP. This complex process is regulated by multiple regulators that serve to tune the signal response.

Extensive experimental and computational research has been performed to analyze BMP regulation in Drosophila, a widely studied model organism, and has advanced our understanding of animal development. Because of …

Evaluation Of A Cool-Season Grass-White Clover Mixture For Low-Nitrogen Input Lawns, Gabriel Adam Macke

Open Access Theses

Turfgrass lawns require supplemental nitrogen (N) to maintain green color and seasonal shoot density. Improper lawn fertilization with excess N or phosphorus has the potential to contaminate both surface and groundwater. Thus, to reduce the reliance on supplemental N fertilization, alternative strategies or novel turf systems like grass-legume mixtures need explored. White clover ( Trifolium repens L.) is a stoloniferous legume that biologically fixes N from the atmosphere and adds N into the soil via mineralization. The objective of this field study was to evaluate the persistence and feasibility of a cool-season grass-clover lawn mixture. A lawn grass mixture with …

Can Wearable Devices Reduce Burnout By Making People Aware Of Stress?, Rohit Mundayaliyath Mundayadan

Open Access Theses

Wearable fitness technology is advancing in its capabilities. Every new sensor collects new health data, and it becomes important to study how effectively this data can be utilized to help people lead healthier lives. The American Psychological Association found that Americans live with stress levels higher than what is considered healthy. Poorly managed stress can lead to burnout, which leads to unproductive workers. Burnout is known to cost businesses considerable money. The goal of this research study was to determine if burnout could be reduced through the use of a consumer wearable device along with smartphone apps that alerted wearers …

Modeling Relative Habitat Suitability And Movement Behavior Of Invasive Burmese Pythons In Southern Florida, Holly E. Mutascio

Open Access Theses

Invasive Burmese pythons are established in the Everglades and are altering the ecology of southern Florida. Their distribution in Florida is expanding northward into more urbanized and fragmented habitats. An understanding of the suitability of habitat throughout southern Florida for Burmese pythons and their interaction with Florida’s landscapes through movement behavior is vital for predicting the python’s ability to persist in habitats outside of the Everglades. In this thesis, we use ecological modeling to predict habitat suitability and to investigate personality-dependent dispersal.

First, we used presence-only ecological niche modeling with correction for sampling bias to identify the key landscape variables …

Structure-Function Analysis Of The Dopamine Transporter In The Presence Of Synthetic Cathinones And Amphetamines, Shari Melissa Radford

Open Access Theses

The dopamine transporter (DAT) is a monoamine transporter that regulates dopamine (DA) neurotransmission by clearing DA from the synapse. DAT is acted on by a number of psychostimulant drugs, which block reuptake and potentiate DA signaling. Several inhibitors of DAT, both amphetamines and cathinones, also cause reversal of transport. Previous research has shown the importance of a conserved salt bridge in another monoamine transporter, the serotonin transporter, in amphetamine-induced efflux. In our studies here, we engineered a mutant Drosophila melanogaster DAT (dDAT D475N) designed to interrupt this salt bridge and examine the effects on amphetamine- and cathinone-induced efflux in vitro. …

Evaluation Of Thermal Process Lethality In Meat For Non-Pathogenic Escherichia Coli As A Surrogate For Salmonella, Morgan Alyse Redemann

Open Access Theses

Non-typhoidal Salmonella is the leading cause of foodborne illness in the United States, resulting in about 20,000 hospitalizations and nearly 380 deaths annually. The meat processing industry has been especially plagued by Salmonella, from meat-inherent sources and more alarmingly, cross-contamination. For ready-to-eat (RTE) meat products specifically, this can cause significant problems in processing facilities ensuring safe product for consumption, resulting in foodborne illness.

The development of standard lethality compliance guidelines by the United States Department of Agriculture Food Safety and Inspection Service (USDA-FSIS) within Appendix A assists processors in confirming that Salmonella is inactivated in RTE beef and poultry products, …

The Impact Of Sleep Disruption On Mouse Physiology, Behavior, And Welfare, Amanda L. Robinson-Junker

Open Access Theses

Laboratory mice are nocturnal, spending most of their daylight hours asleep. But they live in the diurnal world of human investigators and husbandry staff, who primarily work during this rest period. In humans, lack of sleep or sleep that occurs outside the normal circadian sleep period (as in shift work) has adverse effects. These include increased risk of cardiovascular disease, cancer, metabolic disorder, mood disorders, type II diabetes, and obesity. However, it is unknown if mice experience sleep disruption due to these human activities, and, if so, what the adverse effects may be. This is an important question, not only …

Cereal Rye Cover Crop Effects On Soil Physical And Chemical Properties In Southeastern Indiana, Joseph D. Rorick

Open Access Theses

Cover crops are growing in popularity in the Midwest, although questions remain about how to include them most effectively in a corn-soybean ( Zea mays L. - Glycine max L.) rotation. This study was conducted to determine the effects of cereal rye ( Secale cereale L.) on soil bulk density and water retention, soil organic carbon, soil nitrogen, and water stable aggregate mean weight diameter after four years of cover crop growth and the effects on soil moisture over a five year period. The study was conducted at the Southeast Purdue Agricultural Center (SEPAC) on silt loam soils. A 14 …

Recombinant Listeria Adhesion Protein Expressing Probiotics Protect Against Listeria Monocytogenes Infection In Animal Models, Valerie E. Ryan

Open Access Theses

Listeria monocytogenes (Lm) is a foodborne pathogen, found ubiquitously in nature, and has a high morbidity rate among immunocompromised individuals, the elderly, and especially pregnant women and their fetuses resulting in abortion, stillbirth, and neonatal infection. There are currently no preventative medical interventions against Lm infection. The Listeria adhesion protein (LAP) is present in both pathogenic and non-pathogenic Listeria (i.e., L. innocua) and has shown to interact with host epithelial proteins causing tight junction dysregulation aiding in pathogen attachment and paracellular translocation across the host intestinal epithelium. Our lab has demonstrated that recombinant probiotics, Lactobacillus casei (LbcWT) expressing LAP …

A Small-Scale Testbed For Large-Scale Reliable Computing, Jason R. St. John

Open Access Theses

High performance computing (HPC) systems frequently suffer errors and failures from hardware components that negatively impact the performance of jobs run on these systems. We analyzed system logs from two HPC systems at Purdue University and created statistical models for memory and hard disk errors. We created a small-scale error injection testbed—using a customized QEMU build, libvirt, and Python—for HPC application programmers to test and debug their programs in a faulty environment so that programmers can write more robust and resilient programs before deploying them on an actual HPC system. The deliverables for this project are the fault injection program, …

Linking Nanoscale Mechanical Behavior To Bulk Physical Properties And Phenomena Of Energetic Materials, Matthew R. Taw

Open Access Theses

The hardness and reduced modulus of aspirin, RDX, HMX, TATB, FOX-7, ADAAF, and TNT/CL-20 were experimentally measured with nanoindentation. These values are reported for the first time using as-received micron sized crystals of energetic materials with no additional mechanical processing. The results for TATB, ADAAF, and TNT/CL-20 are the first of their kind, while comparisons to previous nanoindentation studies on large, carefully grown single crystals of the other energetic materials show that mechanical properties of the larger crystals are comparable to crystals in the condition they are practically used. Measurements on aspirin demonstrate the variation that can occur between nanoindentation …

Genetic Study Of Carotenoids In Maize Grain (Zea Mays L.), Oscar Rafael Espejel Venado

Open Access Theses

Pro-Vitamin A (proVA) carotenoids, which are converted into retinol (Vitamin A) in the human body, have been the subject of human nutrition studies and are a target for biofortification of staple crops. Historically, β-carotene has been the principal target for enhancing levels of proVA, yet there is recent interest in enhancing the proVA carotenoid β-cryptoxanthin. Studies have shown that β-cryptoxanthin has excellent bioavailability, and its use in maize may be nearly as effective as β-carotene in providing retinol. The primary aim of this study was to gain a better understanding of the genetic control of levels of β-cryptoxanthin, conversion of …

The Role Of Hif1alpha And Hif2alpha In Muscle Development And Satellite Cell Function, Shiqi Yang

Open Access Theses

Hypoxia inducible factors (HIFs) are central mediators of cellular responses to fluctuations of oxygen, an environmental regulator of stem cell activity. Muscle satellite cells are myogenic stem cells whose quiescence, activation, self-renewal and differentiation are influenced by microenvironment oxygen levels. However, the in vivo roles of HIFs in quiescent satellite cells and activated satellite cells (myoblasts) are poorly understood. Expression analyses indicate that HIF1α and HIF2α are preferentially expressed in pre- and post-differentiation myoblasts, respectively. Interestingly, double knockout of HIF1α and HIF2α (HIF1α/2α dKO) in embryonic myoblasts results in apparently normal muscle development and growth. However, HIF1α/2α dKO in postnatal …

Forest Industry Byproducts Improve Soil Quality And Increase Pepper Growth In Three Soils Infested With Phythophthora Blight, Xiaojun Zhao

Open Access Theses

Phytophthora blight is a serious threat to the Midwest vegetable industry, because the oomycete pathogen responsible for this disease, Phytophthora capsici, has a wide host range, can spread quickly in fields, and produces resilient oospores that can survive in soil for years. Phytophthora capsici has become resistant to commonly used fungicides and resistant crop varieties are rare. Amending soil with complex organic substrates has potential to improve soil quality and suppress soil-borne pathogens including P. capsici. Indiana has a significant forest industry with many residual products that could be used as locally available amendments to meet this goal. …

Impact Of Dietary Modification On Aflatoxicosis In Poultry, Xi Chen

Open Access Dissertations

Aflatoxin contamination in feed and feed ingredients is of high worldwide prevalence, posing serious risks to the livestock industry. A series of studies were conducted to better understand the comprehensive impact of aflatoxicosis and its interrelationships with dietary modifications in broiler chicks and ducks. Results of these studies emphasize the many factors that the severity of aflatoxicosis depends on, including animal species, age, presence of mycotoxin adsorbent, and concentration of major dietary nutrients. Exposure to 2 mg/kg cultured AFB1 led to reduced feed intake, BW gain, depressed feed efficiency, increased relative liver weight, negatively affected serum measures and complement activity, …

Mean Hydroclimatic And Hydrological Conditions During Two Climatic Modes In The Devils Lake Basin, North Dakota (Usa), Paul E. Todhunter

Geography & Geographic Information Science Faculty Publications

Proxy variables from palaeolimnological studies of lakes in the Prairie Pothole Region of North America have been used to infer large oscillations during the late Holocene between longer periods of high-salinity–dry conditions and shorter periods of low-salinity–wet conditions producing a normative pattern marked by the absence of hydrological stability. Studies of the historical rise in lake level at Devils Lake have identified 1980 as a transition point between two such hydroclimatic modes. This study uses multiple datasets to characterize the mean hydroclimatological and hydrological conditions of these two climatic modes. Mode 1 is a cool and dry phase, and mode …

Dielectrically-Loaded Cylindrical Resonator-Based Wireless Passive High-Temperature Sensor, Jijun Xiong, Guozhu Wu, Qiulin Tan, Tanyong Wei, Dezhi Wu, Sanmin Shen, Helei Dong, Wendong Zhang

Publications

The temperature sensor presented in this paper is based on a microwave dielectric resonator, which uses alumina ceramic as a substrate to survive in harsh environments. The resonant frequency of the resonator is determined by the relative permittivity of the alumina ceramic, which monotonically changes with temperature. A rectangular aperture etched on the surface of the resonator works as both an incentive and a coupling device. A broadband slot antenna fed by a coplanar waveguide is utilized as an interrogation antenna to wirelessly detect the sensor signal using a radio-frequency backscattering technique. Theoretical analysis, software simulation, and experiments verified the …

Utilization Of Ferrioxamine Microarrays For The Rapid Detection Of Pathogenic Bacteria, Nigam Bir Arora

Open Access Dissertations

Siderophores are low-molecular weight species utilized by bacteria for the sequestration of iron, an essential nutrient. Siderophores and their cognate receptors are considered to be virulence factors, due to their prominent role in pathogenicity. The work presented here focuses on ferrioxamine (FOx) as an “immutable” ligand for pathogen detection. A number of bacterial strains expressing high-affinity FOx receptors were identified by a proteomic BLAST search, and screened against microarrays patterned with FOx conjugates for detection using label-free optical imaging. Aspects such as inkjet printing and surface chemistry, iron-limiting conditions and bacterial selection protocols, and linker conjugate design were addressed and …

A Unique Perspective On The Demand For Livestock Product Attributes, Elizabeth S. Byrd

Open Access Dissertations

Consumers are increasingly concerned about the social and environmental impacts of the foods they purchase. Both choice experiments and best-worst scaling (BWS) methodology have been used to elicit consumer willingness to pay (WTP) for food attributes and the relative importance of food attributes to consumers. The dissertation consists of four essays. Chapter 2 presents a formal law review article exploring the laws associated with wild game harvest, consumption, and inspections. The remaining three essays (Chapters 3-5) each address an extension or application of choice experiment and/or BWS methodology focused on credence attributes of meat products. Two online surveys were used …

Authentic Science In Education: Studies In Course-Based Research At The United States Military Academy, Anthony M. Chase

Open Access Dissertations

This dissertation consists of two studies at the United States Military Academy. Both studies involve the use of Course-based Undergraduate Research Experiences (CUREs). These experiences give students the ability to engage in undergraduate research at an early point in their academic career by replacing traditional laboratory activities with semester-long research projects. Both studies show an implementation of this type of instruction from the Center for Authentic Science Practice in Education (CASPiE). Study 1 shows the specific method of implementation at the military academy and explores learning-based outcomes. Primarily the outcome of critical thinking is demonstrated. Critical thinking is a construct …

Exploring The Potential Impacts Of Climate Change On North America's Laurentian Great Lakes Tourism Sector, Natalie Chin

Open Access Dissertations

Climate change is one of the major challenges facing the global hospitality and tourism sector in the coming century and, given the important role that weather and climate play in all aspects of the tourism experience, tourism businesses owners need to start thinking about and enacting climate change adaptation strategies now. This work has utilized a combination of social science and physical science methods to (1) understand how the Great Lakes tourism sector could be impacted by climate change and (2) provide some insights into how researchers can help business owners prepare for these potential impacts. Overall, the results of …

Essays On Malawian Agriculture: Micro-Level Welfare Impacts Of Agricultural Productivity; Profitability Of Fertilizer Use; And Targeting Of Fertilizer Subsidy Programs, Francis Addeah Darko

Open Access Dissertations

This dissertation comprises of three essays that address different aspects of agriculture in Malawi using a two-wave panel data collected by the National Statistical Office of Malawi with support from the World Bank Living Standards Measurement Study – Integrated Surveys on Agriculture (LSMS-ISA) program. Each essay stands alone as an independent study because of differences in research questions and the methodologies used in addressing the questions.

The first essay analyzes the micro-level welfare impacts of agricultural productivity. Welfare is measured by various dimensions of poverty and food insecurity; and agricultural productivity is measured by maize yield and value of crop …

Nanoparticle Toxicity And Molecular Mechanisms In Fish: A Case Study With Silver Nanoparticles, Jiejun Gao

Open Access Dissertations

Nanoparticles (NPs) are widely used in a myriad of commercial and industrial products making their entry to the environment a likely event. NPs have unique physical-chemical properties that result from their small size and high surface area to volume ratio, making them highly reactive and potentially toxic. In Chapter 1, we summarize the effects and mechanisms of metal-based NPs on the vascular system. In vitro studies have shown that NPs are anti-angiogenic because they cause inflammation, oxidative stress, and apoptosis of endothelial cells resulting in increased permeability and decreased proliferation and migration. Whole animal studies examining the effects of NPs …

The Association Between Tolerance For Ambiguity And Fear Of Negative Evaluation: A Study Of Engineering Technology Capstone Courses, Sergey I. Dubikovsky

Open Access Dissertations

For many students in engineering and engineering technology programs in the US, senior capstone design courses require students to form a team, define a problem, and find a feasible technical solution to address this problem. Students must integrate the knowledge and skills acquired during their studies at the college or university level. These truly integrative design activities do not have a single “correct” solution. Instead, there is an array of solutions, many of which could be used to achieve the final result. This ambiguity can cause students to experience anxiety during the projects.

This study examined the main topics: • …

Bad Girls In Corsets: Women And The Transgressive Body In The Nineteenth Century, Colleen Warwick Green

Open Access Dissertations

Women, and their bodies, posed an increasing anxiety for Victorian society. Culturally and outwardly, the Victorian era strove to maintain a level of decorum that, increasingly, the nineteenth-century woman were, rebelling against. The urge for women to break through social barriers and constraints binding them to the century created a divergence in thought from the traditional mores of the past, in turn affecting the ways in which womens’ bodies were portrayed, displayed and manipulated by the authors and artists of the century.

As women entered actively entered into spaces once closed to them, they furthered the rift of uncertainty and …

Syk Promotes Tgf-Beta-Induced P-Body Clearance In Breast Cancer Cells Through The Enhancement Of Autophagy, Shana D. Hardy

Open Access Dissertations

SYK is a protein tyrosine kinase that plays an essential role in the development and activation of immune cells. Its expression, however, is not limited to immune cells. SYK is expressed in a variety of epithelial cell types and epithelial-derived tumors. Reports regarding the role of SYK expression in these diverse cell types and tumors have been opposing. In breast cancer, SYK expression has been overwhelmingly associated with tumor suppression. The loss of Syk expression is observed in invasive breast carcinoma tissue and cell lines and the reintroduction of Syk into metastatic breast cancer cells suppresses tumor growth and metastasis. …

Concurrent Detection And Isolation Of Cellular And Molecular Biomarkers, Wanfeng Huang

Open Access Dissertations

Detection of cancer markers such as protein biomolecules and cancer cells in bodily fluids is of great importance in early diagnosis, prognosis as well as evaluation of therapy efficacy. Numerous devices have been developed for detecting either cellular or molecular targets, however there has not yet been a system that can simultaneously detect both cellular and molecular targets effectively. Molecule and cell-based assays are important because each type of target can tell a different story about the state of the disease and the two types of information can potentially be combined and/or compared for more accurate biological or clinical assessments. …

Modeling For Thermal Resistance Of Non-O157 Shiga Toxin Producing Escherichia Coli In Ground Beef, Jagpinder S. Brar

Open Access Dissertations

Predictive models in microbiology are used for estimating the growth or survival of microorganism in a set of environmental conditions. A validated predictive model provides an alternative to extensive survival and shelf life studies. In this study, a predictive inactivation model for non-O157 shiga toxin producing Escherichia coli (STEC) in ground beef was developed. Six strains of non-O157 STEC; E. coli O26:H1, E. coli O45:H2, E. coli O103:H2, E. coli O111:H8, E. coli O121:H9, and E. coli O145: nonmotile, has similar pathogenicity as E. coli O157:H7 and can cause serious food borne illnesses. These pathogens are considered as an adulterant …

Exploring The Cause Of Injury Or Death In Grain Entrapment, Engulfment And Extrication, Salah F. Issa

Open Access Dissertations

Grain entrapments and engulfments are one of most common hazards associated with grain storage facilities. Since the 1970’s over 1,880 incidents have been documented in agricultural confined spaces of which 65% of all recorded incidents were grain entrapments and engulfments. There have been several studies conducted on the contributing factors behind these incidents; however, there have been very few attempts to understand the environmental, physiological or psychological factors the victims experience while entrapped, engulfed, or extricated. This includes understanding how secondary injuries are caused by grain or during extrication by first responders. The research effort was divided into three segments. …