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Documenting The Experiences Of Gay Latinos In Higher Education Through The Use Of Testimonio, Lorenzo Fabian Garcia

Doctoral Dissertations

This qualitative study focuses on the stories of six self-Identified Gay Latinos in a higher education. The participant’s stories are documented using Testimonio. The six men were uniquely situated to give their testimonios about their campus experiences of seeking support in that they were the narrators of the experiences. Key findings indicated a pipeline of support which began with supportive families. Multidimensional identity was well defined by the participants as understanding of being both Latino and Gay. The participants, while exploring campus spaces for support, found themselves navigating through one identity or the other resulting in a process of selective …

Ion Mobility-Mass Spectrometry For Structural Characterization And Applied 'Omics: A Study In Neurodegenerative Diseases, James Russell Arndt Iii

Graduate Theses, Dissertations, and Problem Reports (ETD)

Huntington's disease is neurodegenerative disease caused by an expanded polyglutamine-coding CAG repeat in exon 1 of the huntingtin gene. Huntingtin exon 1 forms the primary toxic amyloid structure in Huntington's disease; disease severity is directly correlated with polyglutamine length. Recent works have shown that fully formed amyloid plaques may not represent the most toxic species in Huntington's disease; the most neurotoxic species may be small, diffuse oligomer (4 - 20 monomer units) that are precursors to amyloid plaques. While the polyglutamine region is undisputed as the primary constituent of amyloid structure, aggregation kinetics and morphology are regulated by the presence …

Intimate Empire: Women's Travel Writing And U.S. Imperialism 1880-1920, Andrea Bebell

Graduate Theses, Dissertations, and Problem Reports (ETD)

This dissertation examines how several turn-of-century U.S. women journalists and travel writers represent U.S. imperialism in a range of locales, trafficking in prevailing discourses of gender and race to represent colonized Others and as they engage in a comparative analysis to evaluate women's status in the U.S. In doing so, I suggest that these women, largely overlooked in critical discourses on U.S. imperialism, nonetheless contributed to the U.S. imperial imaginary. By recuperating the work of "newspaper ladies" Margherita Hamm, Nellie Bly, and Mary Krout, and through a reconsideration of Edith Wharton's travel writing, I consider the complex ways that white …

Comparing Diel Cycles Of Dissolved Inorganic Carbon To Diel Cycles Of Fe And Mn At A Coal Mine Drainage Site In Harrison Co., Wv, Jill L. Riddell

Graduate Theses, Dissertations, and Problem Reports (ETD)

Diel (24 hour) cycles of dissolved inorganic carbon and dissolved metals have rarely been studied in concert in coal mine drainage systems containing high CO2. Diel samples were collected from two locations at a site with elevated CO2; the locations differed in their CO 2 concentrations and the amount of vegetation present. Field data and samples were collected from both locations during March, May, and July 2014. To determine if the parameters cycled in a diel fashion, the data were fit using a cosine model and the goodness of fit was determined using an f-test statistic. Overall, 15 of 20 …

Evaluating The Impacts Of Vaccination, Antiviral Treatment And School Closure On Influenza Epidemic, Xiaofei Shi

Graduate Theses, Dissertations, and Problem Reports (ETD)

Multi-objective simulation optimization was performed to synergistically investigate the cost and benefits of the most commonly-used strategies for H1N1 epidemic mitigation: vaccination, antiviral treatment, and school closure. By simultaneously considering the three intervention strategies, this study leads to findings that supplement those in the existing work, and provides additional insights regarding intervention decision making. Specifically, our investigation suggests that different vaccine prioritization strategies, the age-based versus ACIP (Advisory Committee on Immunization Practices) recommendation, be implemented depending on vaccine availability; individual school closure policies are favored over their global counterparts, at least when both vaccination and antiviral treatment are implemented with …

Word Play: Modernist Women And Performative Writing, Kathryn Ridinger Smorul

Graduate Theses, Dissertations, and Problem Reports (ETD)

In Word Play: Modernist Women and Performative Writing I read modernist literary texts informed by theories about theatre, performance, and art. Exploring lesser-known works of Djuna Barnes, Gwendolyn Bennett, Edna St. Vincent Millay, and Mina Loy, I combine literary analysis and performance studies to discover how modernist texts "talk back" to performance theories.;Performance studies, of course, is an inter-discipline that includes anthropology, sociology, performing arts, and literary theory. Modernist authors, influenced as they often are by art, performance, and ritual, incorporate these interdisciplinary interests into a writing practice, specifically performative writing. Performative writing is not so much a matter of …

Soluble Oligomeric Abeta42 Affects Microglia Activation And Mitochondrial Respiration, Cong Tan

Graduate Theses, Dissertations, and Problem Reports (ETD)

Microglia activation is associated with many neurodegenerative diseases such as Alzheimer's disease (AD). When activated, microglia cells change their morphology and become phagocytic. In the Alzheimer's brain, microglia cells play an important role in scavenging plaque-damaged cells and in clearing Abeta plagues. Soluble oligomeric Abeta (oAbeta) is capable to stimulate microglia activation. In AD brain, microglia cells accumulate around senile plaques as a hallmark of the pathology. Recently, we found that mitochondrial oxidative phosphorylation is affected in a dose-dependent manner in a microglia cell line (C8-B4) and primary rat glia cell. Mitochondrial basal, maximal and spare respiration capacities increase at …

Role Of Interference And Computational Complexity In Modern Wireless Networks: Analysis, Optimization, And Design, Salvatore Talarico

Graduate Theses, Dissertations, and Problem Reports (ETD)

Owing to the popularity of smartphones, the recent widespread adoption of wireless broadband has resulted in a tremendous growth in the volume of mobile data traffic, and this growth is projected to continue unabated. In order to meet the needs of future systems, several novel technologies have been proposed, including cooperative communications, cloud radio access networks (RANs) and very densely deployed small-cell networks. For these novel networks, both interference and the limited availability of computational resources play a very important role. Therefore, the accurate modeling and analysis of interference and computation is essential to the understanding of these networks, and …

Testing The Relation Between Disgust And Approach-Avoidance Behavior, Ronald Thomas

Graduate Theses, Dissertations, and Problem Reports (ETD)

The Behavioral Immune System (BIS) is a set of psychological processes that evolved to protect individuals from harmful contaminants and pathogens in the environment (Miller & Maner, 2011; Schaller & Duncan, 2007; Tybur, Lieberman, Kurzban, & DeScioli, 2013). The primary mechanism through which the BIS functions is the emotion of disgust, which causes negative physical and affective reactions that encourage avoidance behavior and serve as a deterrent from approaching potentially harmful stimuli (Schaller & Duncan, 2007). Consequently, disgust is proposed to serve a disease-avoidance mechanism. However, relatively little research has empirically tested the link between disgust and general behavioral avoidance …

"Man Of The House": A Turning Point That Leads To Criminal Behavior?, Delia A. Trickett

Graduate Theses, Dissertations, and Problem Reports (ETD)

This research study seeks to formally test a relationship between household structure, birth order and juvenile delinquency that was suggested as an onset to criminal behavior by men incarcerated in a maximum security facility. The focus of this study is on first born males due to the retrospective narratives given by the incarcerated men that being a first born male who has either lost a father-or father figure due to death, divorce and/or prison have felt a sense of responsibility as the "man of house" to be able to provide family stability which can include care of other siblings, maintaining …

Lithofacies And Sequence Stratigraphy Of The Middle Devonian Marcellus Formation For A Cored Well In Clearfield County, Pennsylvania Using Core Analysis Methods, Chloe S. Wonnell

Graduate Theses, Dissertations, and Problem Reports (ETD)

Seven lithofacies were identified from visual descriptions and quantitative interpretations using traditional core analysis methods, such as core and thin-section description, x-ray diffraction (XRD), total organic carbon (TOC), and spectral core gamma-ray (GR). Ingrain's digital rock physics (DRP) color-coded CoreHDRTM lithofacies classification was successful at illustrating small-scale variations in the core based off high resolution bulk density (RHOB) and photoelectric factor (PEF) logs. A core-scale lithofacies log and spectral core GR aids in recognizing condensed sections and stacking patterns, which helps establish a sequence stratigraphic framework for the cored well that consists of six stratigraphic intervals. The sequence stratigraphic framework …

Mapping And Modeling Soil Organic Carbon In The Eastern Allegheny Plateau And Mountains Using Legacy Data, Katey M. Yoast

Graduate Theses, Dissertations, and Problem Reports (ETD)

The deeply dissected topography and diverse climate of the Eastern Allegheny Plateau and Mountains (Major Land Resource Area (MLRA) 127) create challenges for dynamicpedoecological modeling needed for ecosystem management adaptation to a changing climate. The spatial distribution of soil organic carbon (SOC), one of the most dynamic soil properties, has been previously estimated and mapped using the State Soil Geographical Database (STATSGO2) and the more detailed Soil Survey Geographic Database (SSURGO) for MLRA 127, estimating mean SOC to a depth of 1 m to be 2.60 and 4.40 kg m-2, respectively. Previous studies have shown that these approximations underestimate true …

The Influence Of Accelerometer Cut Points On Determining The Percent Of Preschool-Age Children Meeting Physical Activity Guidelines, Anna Elizabeth Scott

Graduate Theses, Dissertations, and Problem Reports (ETD)

Reduced physical activity is thought to be a major contributor to energy imbalance and obesity in children and adolescents. A challenge for researchers is selecting a tool that will accurately measure physical activity, especially in young children. A current tool that is used is an accelerometer. This study had two objectives. The first examined if different sets of accelerometer cut points developed for preschool-age children would produce the same physical activity results when identical raw data were run through them. Through the use of accelerometer data the second objective aimed to determine the percent of children who met the physical …

It's A Small World: Biogeography And Invasion In The Mouse Intestine, Ellen Lark

Graduate Student Theses, Dissertations, & Professional Papers

Host associated systems are of particular interest to many microbiologists because invasion of these systems can lead to disease. One important host-associated systems is the intestinal microbiome, but in many studies, including those on pathogenesis, this system is represented by samples from one location (generally the feces or cecum). This body of work was initiated in part because I wondered why a large and diverse ecosystem was being was being represented by samples from only one habitat.

The biogeography of living organisms has an impact on landscape ecology studies, including those in the field of invasion ecology. Despite several studies …

A Novel Computational System For Identification Of Biological Processes From Multi-Dimensional High-Throughput Genomic Data, Julian Marshall Dymacek

Graduate Theses, Dissertations, and Problem Reports (ETD)

Identifying potential toxicity signaling pathways could guide future animal studies and support human risk assessment and intervention efforts. This thesis describes a novel computational approach for identifying biological processes and pathways that are significantly associated with a disease pathology from time series, dose response, gene expression data.;Our system employs a novel constrained non-negative matrix factorization algorithm and Monte Carlo Markov chain simulation to identify underlying patterns in mRNA gene expression data. Quantitative pathology can be used as a pattern constraint. The found patterns can be thought of as functions that influence a gene's expression. Using a database of curated gene …

Institutional Decolonization: The Internationalization Of The Conflict Over Organized Labor In British Guiana, 1946-1961, Joshua David Esposito

Graduate Theses, Dissertations, and Problem Reports (ETD)

This dissertation argues that an internationalized struggle for control of British Guiana's trade unions after 1946 resulted in institutional dysfunction and violence in the early 1960s. Some Guianese nationalists, who eventually became leaders of the People's Progressive Party (PPP), wanted to replace the institutions imported from Great Britain with trade unionism geared towards a Guianese version of communism. Conversely, reformist Guianese labor leaders were intent on establishing a left-leaning capitalist framework of labor relations, primarily based on a British model of unionism. Guianese unionists' ideological proclivities shaped their international affiliations, adding a transnational dimension to the struggle between the colony's …

Cholesterol Modulates Huntingtin Binding To And Aggregation On Lipid Membranes, Xiang Gao

Graduate Theses, Dissertations, and Problem Reports (ETD)

Huntington disease is an autosomal dominant neurodegenerative disorder. The abnormally long CAG-repeats in the huntingtin gene that encode an expanded polyglutamine stretch which promotes self-assemble of huntingtin into different aggregation species that are disease related. Huntingtin intimately interacts with a variety of lipid membranes. Lipid composition is altered in HD, especially cholesterol content. Here we investigate how cholesterol content modulates the interaction between huntingtin and lipid membranes. TBLE/PDA binding assay is performed to test the binding affinity of huntingtin with lipid bilayers containing different amount of cholesterol. As the cholesterol content increases, the extent of huntingtin binding to lipid bilayers …

Saprophytic Colonization And Sporulation Of Virulent And Hypovirulent Cryphonectria Parasitica On American Chestnut (Castanea Dentata) And Scarlet Oak (Quercus Coccinea), Eric Shelton Goddard

Graduate Theses, Dissertations, and Problem Reports (ETD)

Colonization and sporulation (stroma production) of virulent (V) and hypovirulent (HV) Cryphonectria parasitica, were evaluated on two hosts to better understand the saprophytic stage of the fungus and its ability to produce HV inoculum. Both the V and HV C. parasitica strains were isolated from an existing chestnut orchard at the test site. Castanea dentata (American chestnut) and Quercus coccinea (scarlet oak) were used as test hosts. Sixty centimeter long stems of both species were cut from saplings and placed as pairs in three-layer, triangular stacks. Each stack was wound inoculated with either V, HV or water agar (control) inoculum. …

Development Of A Conservation Action Plan For The Eastern Hellbender (Cryptobranchus Alleganiensis Alleganiensis) In West Virginia, William J. Greathouse

Graduate Theses, Dissertations, and Problem Reports (ETD)

The Eastern hellbender (Cryptobranchus alleganiensis alleganiensis ) is the largest salamander in North America growing to 75 centimeters in length. This fully aquatic species is an indicator of good stream and river quality and has been considered to be in decline or rare throughout its range. This study focused on the current distribution of the Eastern hellbender at sites of historic occurrence in West Virginia in order to determine the species' current range and factors impacting its distribution in order to facilitate planned reintroductions and translocations of hellbenders reared in human care. The success of the reintroduction and translocation efforts …

Assessing Feeder Space Access And Commercial Phytase Production Methods On Performance Of Commercial Broilers Fed Pelleted Diets, Mark Edward Lemons

Graduate Theses, Dissertations, and Problem Reports (ETD)

Applied research conducted at universities is essential for further advancements in poultry science and ultimately the commercial poultry industry. One important area of applied nutrition research is examining production methods associated with the important feed additive/enzyme phytase. Exogenous phytase inclusion in poultry diets has consistently been associated with decreased diet cost, increased broiler performance, and reduction in negative environmental impact. However, thermal stability of commercial phytases are variable. Strategies for production of phytases that ultimately maintain activity after being subjected to the pelleting process is essential. Phytase manufacturers have modified production methods in an effort to maximize phytase thermal stability …

Rotating Space Elevators: Classical And Statistical Mechanics, Steven Knudsen

Graduate Theses, Dissertations, and Problem Reports (ETD)

We investigate a novel and unique dynamical system, the Rotating Space Elevator (RSE). The RSE is a multiply rotating system of strings reaching beyond the Earth geo-synchronous satellite orbit. Objects sliding along the RSE string ("climbers") do not require internal engines or propulsion to be transported far away from the Earth's surface. The RSE thus solves a major problem in the space elevator technology which is how to supply the energy to the climbers moving along the string. The RSE is a double rotating floppy string. The RSE can be made in various shapes that are stabilized by an approximate …

Metal Oxide Nanoparticles: The Importance Of Size, Shape, Chemical Composition, And Valence State In Determining Toxicity, Katherine Dunnick

Graduate Theses, Dissertations, and Problem Reports (ETD)

Nanoparticles, which are defined as a structure with at least one dimension between 1 and 100 nm, have the potential to be used in a variety of consumer products due to their improved functionality compared to similar particles of larger size. Their small size is associated with increased strength, improved catalytic properties, and increased reactivity; however, their size is also associated with increased toxicity in vitro and in vivo. Numerous toxicological studies have been conducted to determine the properties of nanomaterials that increase their toxicity in order to manufacture new nanomaterials with decreased toxicity. Data indicates that size, shape, chemical …

Dysregulation Of Micrornas In Blood As Biomarkers For Diagnosing Prostate Cancer, Rhonda W. Daniel

Theses and Dissertations

Prostate cancer is the most common noncutaneous cancer among men, yet current diagnostic methods are insufficient and more reliable diagnostic markers need to be developed. The answer that can bridge this gap and enable more efficient diagnoses may lie in microRNAs. These small, single stranded RNA molecules impact protein expression at the translational level and regulate important cellular pathways. Dysregulation of these small RNA molecules can have tumorigenic effects on cells and lead to many types of cancers.

Currently the Prostate-Stimulating Antigen (PSA) is used as a diagnostic marker for prostate cancer. However, many factors can elevate PSA levels such …

Synthesis, Surface Functionalization, And Biological Testing Of Iron Oxide Nanoparticles For Development As A Cancer Therapeutic, Stanley E. Gilliland Iii

Theses and Dissertations

Iron oxide nanoparticles are highly researched for their use in biomedical applications such as drug delivery, diagnosis, and therapy. The inherent biodegradable and biocompatible nanoparticle properties make them highly advantageous in nanomedicine. The magnetic properties of iron oxide nanoparticles make them promising candidates for magnetic fluid hyperthermia applications. Designing an efficient iron oxide nanoparticle for hyperthermia requires synthetic, surface functionalization, stability, and biological investigations. This research focused on the following three areas: optimizing synthesis conditions for maximum radiofrequency induced magnetic hyperthermia, designing a simple and modifiable surface functionalization method for specific or broad biological stability, and in vitro and in …

Hif-Independent Responses In Hypoxia, Divya Padmanabha

Theses and Dissertations

The adaptive response to hypoxia is accompanied by widespread transcriptional changes that allow for prolonged survival in low oxygen. Many of these changes are directly regulated by the conserved hypoxia-inducible factor-1 (HIF-1) complex; however, even in its absence, many oxygen-sensitive transcripts in Caenorhabditis elegans are appropriately regulated in hypoxia. To identify mediators of these non-HIF-dependent responses, I established a hif-1 mutant reporter line that expresses GFP in hypoxia or when worms are treated with the hypoxia mimetic cobalt chloride (cobalt chloride). The reporter is selective and HIF-independent, in that it remains insensitive to a number of cellular stresses, but is …

Heterosandwich Assay Of Nicotinic Acetylcholine Receptors, Augustine J. Pagan Iv

Theses and Dissertations

Using the technology afforded by Winschel et al., cyclen-1, a high affinity, strong complexation agent for 8-hydroxypyrene-1,3,6 trisulfonate and derivatives, a new assay has been developed for fluorescently labeling proteins of interest (POIs). Ligation of the endogenous ligand for nicotinic acetylcholine receptors (nAChRs), acetylcholine, using click chemistry afforded the triazole derivative of an alkynyl-acylcholine (compound 1) with 8-azidopyrene-1,3,6 trisulfonate (compound 2). Liposomes encapsulated with Rhodamine B were used to strengthen the initial fluorophore response of compound 2, using an anchored form of cyclen-1 complex. Using a palmitoyl tail as the lipophilic moiety for liposomal amplification, the …

Git And Github For Librarians, Robin Camille Davis

Publications and Research

One of the fastest-growing professional social networks is GitHub, an online space to share code. GitHub is based on free and open-source software called Git, a version control system used in many digital projects, from library websites to government data portals to scientific research. For projects that involve developing code and collaborating with others, Git is an invaluable tool; it also creates a backup system and structured documentation. In this article, we examine version control, the particulars of Git, the burgeoning social network of GitHub, and how Git can be an archival tool.

Potential Tau Directed Imaging Agents, Amanda Merrill Boyer

Browse all Theses and Dissertations

Given the ambident reactivity of oxindoles in terms of N- versus C-alkylation, realization of the three points of diversity available to (presumably benzenoid substituted) benzylidene oxindoles is normally achieved via a sequence involving initial N-alkylation of the corresponding isatin precursor, reductive deoxygenation to the N-alkylated oxindole, and finally aldol condensation to the desired target. Since benzenoid substituted isatins are generally more readily available than the corresponding oxindoles, an alternate but much less examined manifold involves reduction of the isatin to an oxindole, aldolization at the C-3 position followed by N-alkylation of the resultant benzylidene oxindole. Since benzylidene oxindoles have potential …

Measuring The Acute Physiological Effects Of Leptin In The Carotid Body, Richard Laurence Pye

Browse all Theses and Dissertations

Obesity is the healthcare crisis of our generation. Circulating levels of the adipokine leptin are proportional to adiposity and in addition to its role as a satiety factor leptin has been shown to have a host of pleiotropic effects. Among these effects is its role as a respiratory stimulant where it has been shown to have both whole body and central stimulatory effects on breathing. Porzionato et al. (2011) showed that leptin and leptin receptors are selectively expressed in the chemosensing type I cells of the carotid body. Carotid bodies are small chemosensitive organs that act as the primary peripheral …

The Guinea Pig Model For Organophosphate Toxicology And Therapeutic Development, Christopher Daniel Ruark

Browse all Theses and Dissertations

Organophosphates (OPs) are highly toxic insecticides and nerve agents that have been designed to inhibit the hydrolysis of acetylcholine by binding to the serine active site of acetylcholinesterase (AChE). They are one of the most common causes of human poisoning worldwide and are frequently intentionally used in suicides in agricultural areas. For this reason, there is a need for therapeutics to rescue those from intoxication. Obvious ethical concerns prevent humans from being subjected to OP exposure for therapeutic efficacy and safety testing. Therefore, animal surrogates for humans must be appropriately selected. A new paradigm, described herein, incorporating both in silico …