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Altered Intraerythrocytic Development Phenotypes Of Artemisinin-Resistant Plasmodium Falciparum Confer A Fitness Advantage, Amanda Hott

USF Tampa Graduate Theses and Dissertations

Resistance to artemisinin combination therapies (ACTs) has emerged in southeast Asia threatening the most widely used treatment against antimalarial-resistant Plasmodium falciparum worldwide. Artemisinin resistance has been associated with a reduced rate of parasite clearance following treatment with an ACT and is attributed to increased survival of ring-stage parasites. Single nucleotide polymorphisms (SNPs) in kelch gene (K13) has been associated with delayed in vivo clearance half-life of artemisinin-resistant P. falciparum and is the only known molecular marker of resistance. The absence of reliable in vitro phenotypes for artemisinin resistance has limited our understanding of the resistance mechanism(s) and fitness costs, therefore …

Development Of Orally Bioavailable 4(1H)-Quinolones And 1,2,3,4-Tetrahydroacridin-9(10H)-Ones With Potent Anti-Malarial Activity, Jordany Richarlson Maignan

USF Tampa Graduate Theses and Dissertations

Although Malaria rates are on the decline due to the efforts of the World Health Organization and other organizations dedicated to the eradication of this disease, a relaxed attitude towards the development of new antimalarial entities would be flawed. Due to the emergence of resistance in the parasite, the almost 50% world-wide reduction in malarial death rates that have been produced over the past 15 years are threatening to be lost

New drugs are urgently needed and our approach focuses on the re-evaluation and optimization of the historic antimalarial ICI 56,780. Due to its causal prophylactic activity, along with its …

Association Between Toxocariasis And Cognitive Function In Young To Middle-Aged Adults, Bruce L. Brown, Lance D. Erickson, Shawn D. Gale, Andrew Berrett, Dawson W. Hedges

Faculty Publications

The ascarid nematodes Toxocara canis (Werner, 1782) and Toxocara cati (Schrank, 1788) may infect humans resulting in toxocariasis. A prior study associated species of Toxocara Stiles, 1905 with cognitive deficits in children. To determine if a similar association between toxocariasis and cognition exists in adults, we analysed a large dataset from the United States’ Center for Disease Control’s National Health and Nutrition Examination Survey. We used linear-regression and multivariate models to examine the association between toxocariasis as assessed by the presence of anti-Toxocara IgG antibodies and three measures of cognitive function – simple reaction time (SRT), symbol-digit substitution (SDS) and …

Interaction Between Helicobacter Pylori And Latent Toxoplasmosis And Demographic Variables On Cognitive Function In Young To Middle-Aged Adults, Bruce L. Brown, Shawn D. Gale, Lance D. Erickson, Dawson W. Hedges

Faculty Publications

Helicobacter pylori and latent toxoplasmosis are widespread diseases that have been associated with cognitive deficits and Alzheimer’s disease. We sought to determine whether interactions between Helicobacter pylori and latent toxoplasmosis, age, race-ethnicity, educational attainment, economic status, and general health predict cognitive function in young and middle-aged adults. To do so, we used multivariable regression and multivariate models to analyze data obtained from the United States’ National Health and Nutrition Examination Survey from the Centers for Disease Control and Prevention, which can be weighted to represent the US population. In this sample, we found that 31.6 percent of women and 36.2 …

Refraction In Mozambique: Evaluations Of Practice And Development Of Competency Frameworks For Eye Care Personnel, Kajal Shah

Doctoral

Purpose The development of competency- based education for optometrists and mid-level eye-care personnel has been identified as an important component in the elimination of avoidable blindness and vision impairment. The Mozambique Eye care Project (MEP) is a multi-institutional collaboration, which seeks to facilitate greater access to training in eye health professions, which will ultimately contribute to providing affordable and accessible eye care within the public health system in Mozambique. An important tenet of the MEP is to develop and enhance the refraction training of all existing (ophthalmic technicians) and new eye care personnel (four-year training of optometrists and eighteen-month training …

Common Psychosocial And Spiritual Factors Among Individuals Who Have Healed From Chronic Lyme Disease, Frederick Green

Antioch University Dissertations & Theses

This dissertation examined the subjective experience of individuals who have healed from Chronic Lyme Disease (CLD). Despite significant attention paid to the controversy over CLD diagnosis and treatment, scholarly research has neglected the psychosocial and/or spiritual factors that facilitate healing from the perspective of CLD sufferers. This study addressed this research gap by using Interpretive Phenomenological Analysis (IPA) to explore the barriers and facilitators of healing among those who have healed from CLD. Six participants who had suffered from CLD and considered themselves healed from the disease were recruited and interviewed. The qualitative data was reduced to meaning units, which …

An Integrated Continuous Class-${\Rm F} ^{-1}$ Mode Power Amplifier Design Approach For Microwave Enhanced Portable Diagnostic Applications, Azeem Imtiaz, Jonathan Lees, Heungjae Choi, Lovleen Tina Joshi

Peninsula Dental School

No abstract provided.

Nurses' Perceptions Of Using An Evidence-Based Care Bundle For Initial Emergency Nursing Management Of Patients With Severe Traumatic Brain Injury: A Qualitative Study, Jintana Damkliang, Julie Considine, Bridie Kent, Maryann Street

School of Nursing and Midwifery

No abstract provided.

Social Networks In Changing Environments, A. D.M. Wilson, S Krause, I. W. Ramnarine, K. K. Borner, R. J.G. Clément, R. H.J.M. Kurvers, J Krause

School of Biological and Marine Sciences

No abstract provided.

Semiparametric Analysis In Conditionally Independent Multivariate Mixture Models, T. Wrobel, Denis H. Y. Leung, J. Qin, T. Hettmansperger

Research Collection School Of Economics

The conditional independence assumption is commonly used in multivariate mixture models in behavioral research. We propose an exponential tilt model to analyze data from a multivariate mixture distribution with conditionally independent components. In this model, the log ratio of the density functions of the components is modeled as a quadratic function in the observations. There are a number of advantages in this approach. First, except for the exponential tilt assumption, the marginal distributions of the observations can be completely arbitrary. Second, unlike some previous methods, which require the multivariate data to be discrete, modeling can be performed based on the …

Digital Medicine, The Fda, And The First Amendment, Adam Candeub

Georgia Law Review

Digital medicine might transform healthcare more fundamentally than the introduction of anesthesia or germ basis theory of disease. Already, tens of thousands of "medical apps" are available for smartphones. These computer applications can measure blood pressure, pulse, lung function, oxygenation level, sugar level, breathing rate and body temperature-and can even diagnose skin cancer, analyze urine, and take an echocardiogram. In fall 2013, the Federal Drug Administration (FDA) asserted regulatory authority over mobile medical applications and other digital medical services, threatening to chill, if not, destroy this innovation. This Article argues that the FDA stands on firm legal ground regulating medical …

Testing The Relation Between Disgust And Approach-Avoidance Behavior, Ronald Thomas

Graduate Theses, Dissertations, and Problem Reports (ETD)

The Behavioral Immune System (BIS) is a set of psychological processes that evolved to protect individuals from harmful contaminants and pathogens in the environment (Miller & Maner, 2011; Schaller & Duncan, 2007; Tybur, Lieberman, Kurzban, & DeScioli, 2013). The primary mechanism through which the BIS functions is the emotion of disgust, which causes negative physical and affective reactions that encourage avoidance behavior and serve as a deterrent from approaching potentially harmful stimuli (Schaller & Duncan, 2007). Consequently, disgust is proposed to serve a disease-avoidance mechanism. However, relatively little research has empirically tested the link between disgust and general behavioral avoidance …

Antimicrobial Effectiveness Of Citrus Essential Oils And Organic Acids Against Salmonella And Listeria Monocytogenes, Karina Walker

Graduate Theses, Dissertations, and Problem Reports (ETD)

The development of natural antimicrobials for foods is of increasing interest as consumers' desire for more natural foods continues to rise. Organic acids and citrus essential oils (EOs) are natural, generally regarded as safe substances that are known to possess antimicrobial properties. In-vitro tests of natural antimicrobials against a range of bacteria are required in order to determine their feasibility for food applications. The objective of this study was to determine the antimicrobial activity of citrus essential oils and organic acids against Salmonella Baildon, Salmonella Enteritidis, Salmonella Typhimurium (ATCC 14028) and three strains of Listeria monocytogenes (ATCC 19115, ATCC 15313, …

Cellulose Based Materials As A Template For The Synthesis Of Copper Nanoparticles For Antimicrobial Applications, Tuhua Zhong

Graduate Theses, Dissertations, and Problem Reports (ETD)

Copper has been used as a broad-spectrum biocide for centuries, showing effective antimicrobial performance against bacteria, fungi and viruses. Among various forms of copper, copper nanoparticles is attracting particular attention because of its high surface area and crystal morphology that will enhance copper antimicrobial properties. However, the high tendency of copper particles to aggregate at a nanoscale remains as a drawback for the development of copper-based nanostructured products. In this research, cellulose-based materials were utilized as a template and stabilizer for copper nanoparticles, and the resulting hybrid material was used as bi-functional material in thermoplastic resins improving the final antimicrobial …

Impacts Of Multiple Stressors On Dryland Vulnerability And Land Conflict Among Border Communities, Alvin Maingi Solomon

Graduate Theses, Dissertations, and Problem Reports (ETD)

This study examines how individuals and groups respond to prevailing politico-economic and biophysical changes and how the adjustments they make influence and are influenced by their social relations and material condition in spatial and temporal terms. This dissertation comprises of three parts: The first part is a critique of the climate-conflict relationship debate and the problems associated with studies pushing for a causal relationship between climate variables and conflict. I argue that scholars lack of attention on varying institutional capacities and the focus solely on environmental factors as trigger mechanisms for conflict leads to misleading arguments about conflict distribution and …

Identification And Biosensing Application Of Molecular Recognition Elements, Ka Lok Hong

Graduate Theses, Dissertations, and Problem Reports (ETD)

Molecular recognition elements (MREs) are biomolecules such as single-stranded DNA (ssDNA), RNA, small peptides and antibody fragments that can bind to user defined targets with high affinities and specificities. This binding property allows MREs to have a wide range of applications, including therapeutic, diagnostic, and biosensor applications. The identification of MREs can be achieved by using the process called Systematic Evolution of Ligands by Exponential Enrichment (SELEX). This process begins with a large library of 109 to 1015 different random molecules, molecules that bind to the user defined target or positive target are enriched in the process. Subsequently, this process …

Amphibian Metamorphosis In Created And Natural Wetlands, Lauren A. Mcpherson

Graduate Theses, Dissertations, and Problem Reports (ETD)

Functional assessment of created wetlands is an important factor in monitoring the success of wetland mitigation projects. Determining the ability of a created wetland to replace lost wildlife habitat and to support productive wildlife populations should be a priority in the assessment of created wetland success. We used a mesocosm design featuring water collected from 3 created wetlands and 3 natural wetlands in West Virginia to evaluate how the water quality from the 2 wetland types were able to support metamorphosis in larval spring peepers (Pseudacris crucifer) and wood frogs ( Rana sylvatica). Spring peepers displayed similar metamorphosis rates in …

Evaluating The Impacts Of Vaccination, Antiviral Treatment And School Closure On Influenza Epidemic, Xiaofei Shi

Graduate Theses, Dissertations, and Problem Reports (ETD)

Multi-objective simulation optimization was performed to synergistically investigate the cost and benefits of the most commonly-used strategies for H1N1 epidemic mitigation: vaccination, antiviral treatment, and school closure. By simultaneously considering the three intervention strategies, this study leads to findings that supplement those in the existing work, and provides additional insights regarding intervention decision making. Specifically, our investigation suggests that different vaccine prioritization strategies, the age-based versus ACIP (Advisory Committee on Immunization Practices) recommendation, be implemented depending on vaccine availability; individual school closure policies are favored over their global counterparts, at least when both vaccination and antiviral treatment are implemented with …

Reversible Phospholipid Nanogels: An Alternative Matrix For Tunable High Resolution Dna Sieving With Enhanced Separation Efficiency And Accurate Extended Range Sizing By Capillary Gel Electrophoresis, Brandon C. Durney

Graduate Theses, Dissertations, and Problem Reports (ETD)

In an aqueous solution the phospholipids dimyristoyl-sn-glycero-3- phosphocholine (DMPC) and 1,2-dihexanoyl-sn-glycero-3-phosphocholine (DHPC) self-assemble to form thermo-responsive non-Newtonian fluids (i.e., pseudo-gels) in which small temperature changes of 5-6 °C decrease viscosity dramatically. This characteristic is useful for sieving-based electrophoretic separations (e.g., of DNA), as the high viscosity of linear sieving additives, such as linear polyacrylamide or polyethylene oxide, hinders the introduction and replacement of the sieving agent in microscale channels. While a practical advantage of utilizing phospholipid pseudo-gels for sieving is the ease with which they are introduced into the separation capillary, the separation performance of the material for DNA analyses …

Identification Of A Critical Gene In The Dihydroergot Alkaloid Pathway, Yulia Bilovol

Graduate Theses, Dissertations, and Problem Reports (ETD)

Ergot alkaloids, bioactive compounds produced by some species of fungi, have had significant impacts on agriculture and medicine. Claviceps purpurea and Epichloe spp. are ergot alkaloid producers associated with agricultural commodities and negatively affect humans and grazing animals, respectively. Another ergot alkaloid-producing fungus called Aspergillus fumigatus is ubiquitous in the environment; infections arising after inhaling the spores may kill immunocompromised persons. Ergot alkaloids and their derivatives are utilized by the pharmaceutical industry for the development of drugs. Some of these drugs are derived from lysergic acid, whereas others are derived from dihydrolysergic acid. Lysergic acid derivatives have vasoconstrictive properties and …

Correlation Analysis Of Climatic Variables, Migration And Dengue Cases In Southeast Florida, Brunilda Lugo

Walden Dissertations and Doctoral Studies

Dengue fever is a debilitating, viral, mosquito-borne disease occurring in tropical and subtropical areas in the world. The majority of dengue cases in the United States were acquired in endemic areas by travelers or immigrants. However, in recent years, autochthonous (locally acquired) dengue cases have been diagnosed in Florida. The purpose of this study was to find an association between potential risk factors and the expansion of dengue fever in the United States. Guided by the eco-bio-social framework, which offers a broad assessment of risk factors for the illness, a retrospective design was used with archival data to correlate changes …

Physicians' Perceptions And Practice Regarding The Prevention Of Catheter-Associated Urinary Tract Infections In The Icu, Marilyn Keng-Nasang Mbi Feh

Walden Dissertations and Doctoral Studies

Catheter associated urinary tract infection (CAUTI) incidence continue to rise despite all prevention efforts. The state of Georgia incidence of CAUTI between 2012 and 2013 showed an increase by 350 cases. The challenge is translating CAUTI prevention knowledge into practice by all physicians. The purpose of this correlational study was to improve the epidemiological understanding of CAUTI. Looking at physicians’ perception and practice of CAUTI preventions was necessary. A total of 336 physicians from the state of Georgia completed a 26-item survey. Additionally, a pilot study was conducted on a small sample of participants. The result of the Cronbach alpha …

Association Of Bisphenol A And C-Reactive Protein Concentrations With Cardiovascular Diseases, Hassan Salim Naji

Walden Dissertations and Doctoral Studies

Bisphenol A (BPA), a widely used chemical in plastic, has drawn wide attention due to its presence in many consumer products and the environment. The purpose of this study was to examine the association between urinary BPA and the reporting of cardiovascular diseases (CVD), and then to examine the effect of C-reactive protein (CRP) as a moderating variable. The data used in this research were extracted from the National Health and Nutrition Examination Survey collected in 2009-2010. Guided by the advanced epidemiological triangle, analysis involved 2 stepwise binary logistic regressions. The first step suggested that the controls were significant in …

Social Network Correlates Of Hcv And Hiv Transmission Risk Behaviors Among Injecting Drug Users, Victor Emanuel Reyes-Ortiz

Walden Dissertations and Doctoral Studies

Drug injection is an increasingly important risk factor in the transmission of blood-borne pathogens, including the hepatitis C virus (HCV) and human immunodeficiency virus (HIV). The purpose of the study was to examine the influence of social network factors on HCV and HIV. The study was grounded in social network theory and sought to determine whether social network characteristics affect high-risk sexual and drug injection behavior as well as self-reported HIV and HCV status. The study design was a quantitative cross-sectional survey. A total of 181 participants in a needle exchange program completed a survey in Spanish assessing individual drug …

Perceptions Of Home And Small Business Owners On Insurance In Accra, Ghana, Helen Stevens-Benefo

Walden Dissertations and Doctoral Studies

Insurance is a prudent option to mitigate business risk and property loss caused by natural disasters. The current study took place in Accra, Ghana, and was prompted by the low adoption of insurance and limited understanding by home and small business owners of its risk mitigation value. The purpose of this multiple-case study was to explore information insurance companies' need to motivate investment in weather-related insurance. The interview questions of the study served to explore the reasons business owners and homeowners purchase or decline insurance, including their understanding of and receptivity to insurance. The theoretical underpinning of this study included …

Climate Change Implications For Health-Care Waste Incineration Trends During Emergency Situations, Emilia Mmbando Raila

Walden Dissertations and Doctoral Studies

Healthcare waste (HCW) incineration practices in the global South countries are among the major sources of black carbon (BC) emissions or smoke. This study analyzes HCW incineration trends during emergency situations and smoke from HCW incineration processes in Haiti. The study was prompted by the current arguments about the climate change and the growing health effects associated with BC emissions. The conceptual framework was based on both adverse health effects from BC emissions exposure and climate change potential of BC emissions. Therefore, the goal was to determine whether cardboard HCW sharps containers emit lower BC emissions to the atmosphere during …

Contextual Factors And Direct Exposure To Hiv: Influences On Youth Sexual Intention, Hadiza Ladidi Osuji

Walden Dissertations and Doctoral Studies

Youth are having sexual intercourse at an early age and as such, are engaging in risky behaviors that are associated with adverse health outcomes, including HIV infection. Little research has been undertaken to examine the influence of contextual factors on youth intentions to delay sexual involvement. To address that gap, this study aimed to examine the influence of age, gender, race, immigration status, HIV knowledge, HIV-related stigma, concern about HIV, direct exposure to HIV/AIDS, peer pressure, and religious influence on youth intentions to delay sexual involvement. Delayed sexual involvement was conceptualized as youth attitudes towards abstaining from sex, their knowledge …

A Survey Of Mathematical Models Of Dengue Fever, Iurii Bakach

College of Graduate Studies: Theses & Dissertations

In this paper, we compare and contrast five models of Dengue fever. We evaluate each model using different scenarios and identify the strenghts and wecknesses of each of the model

Public Health Implications Associated With The Practice Of Utilizing Tires To Singe Meat In Three Major Cities Of Ghana: A Concurrent Mixed Methods Study, Tempest D. Hill

College of Graduate Studies: Theses & Dissertations

Utilization of automobile tires to singe meat (e.g., cows) intended for human consumption presents public health risks due to hazardous chemicals released into the environment and meat. This has become a common practice in some African countries such as Ghana. The objective of this study was to investigate the awareness and perception of the residents in three major cities of Ghana (Cape Coast, Kumasi and Accra) using the Health Belief Model and Social Ecological Model. Survey participants (n=196) and focus group discussion members (n=37) completed the study. Data collected from study participants included meat consumption, awareness of the use of …

Identification Of The Molecular Target Of The Doc Toxin Of The P1 Bacteriophage Plasmid Addiction System, Darren Ferree

Theses

Doc toxin from the bacteriophage P1 plasmid addiction operon kills daughter cells that are cured of plasmid. At the beginning of this project it was known that Doc is a kinase that transfers the phosphoryl group from the gamma position of ATP to some unidentified target protein of approximately 45 kDa molecular weight. To better characterize this target, assays were conducted to determine optimal conditions for Doc-dependent phosphorylation of target, and these conditions were then used to generate samples for analysis by two-dimensional polyacrylamide gel electrophoresis, liquid chromatography-coupled tandem mass spectrometry, and immunoprecipitation. The results of these experiments indicate that …