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Strategies For Preventing Age And Neurodegenerative Disease-Associated Mitochondrial Dysfunction, Vedad Delic

USF Tampa Graduate Theses and Dissertations

Mitochondrial dysfunction plays a pivotal role in the development of aging phenotypes and aging-associated neurodegenerative disorders, such as Alzheimer’s disease (AD), Parkinson’s disease (PD) and Amyotrophic lateral sclerosis (ALS). Strategies that restore mitochondrial dysfunction may rescue the deficits of central metabolism in these disorders and improve cell survival. For example, we found that modulating the mTOR signaling pathway in a tissue culture model of aging-induced mitochondrial DNA mutation enhanced mitochondrial function as evidenced by increased oxygen consumption. Our previous melatonin studies also led us to hypothesize that caloric restriction and the hormone melatonin would reverse brain mitochondrial dysfunction in animal …

The Continuum Of Ethno-Racial Socialization: Learning About Culture And Race In Middle-Class Latina/O Families, Maria D. Duenas

USF Tampa Graduate Theses and Dissertations

This thesis examines the discursive messages and specific practices that Latino families use to transmit messages about culture, race, and racism. Scholars have not fully explored the complexity and range of practices and discourses that are involved in Latinos’ ethno-racial socialization. The use of the phrase “ethno-racial socialization” is important because it combines the concepts of racial socialization and ethnic socialization in an effort to account for how the lived experiences of Latinos who mostly think of themselves as a racial group, are treated as one race, and thus, discuss race with family members. This research explores this process using …

Compassion Fatigue And Self-Care Strategies Among Addiction Professionals: A Qualitative Study, Amanda Depippo

USF Tampa Graduate Theses and Dissertations

Addiction is a prevalent disorder and is on the rise. Addiction has serious symptomology and can negatively impact an individual’s life in several areas. Not only can the person addicted become negatively impacted, but the addiction professional that treats this disorder can become negatively impacted as well. Because there is often a high co-occurrence of addiction and trauma among addiction clients, this intensifies the clinical profile of such clients which compounds the risk for addiction professionals. It is the addiction professional’s response to this combination of addiction and trauma that can cause these professionals to experience compassion fatigue.

Research has …

Genomic Aberrations At The 3q And 14q Loci: Investigation Of Key Players In Ovarian And Renal Cancer Biology, Punashi Dutta

USF Tampa Graduate Theses and Dissertations

Genomic aberrations are primary contributors to the pathophysiology of cancer [11]. Dysregulated expression of genes located within these aberrations are important predictors of chemoresistance, disease prognosis, and patient outcome [12]. This dissertation is focused on understanding the regulation and/or functions of specific genes located at dysregulated genomic regions such as 3q26 and 14q32 in the biology of ovarian and renal cancer, respectively.

Serous epithelial ovarian cancer (EOC) manifest amplification at the 3q26.2 locus [2], an observation consistent with the cancer genome atlas (TCGA) [13]. The most amplified gene in this region is EVI1 which has been extensively studied in hematological …

The Effects Of Supplemented Metabolites On Lifespan And Stress Response Pathways In Caenorhabditis Elegans, Clare B. Edwards

USF Tampa Graduate Theses and Dissertations

Understanding how metabolites contribute to anaplerosis, antioxidant effects, and hormetic pathways during aging is fundamental to creating supplements and dietary habits that may decrease age-associated disease and decline, thus improving the quality of life in old age. In order to uncover metabolic pathways that delay aging, the effects of large sets of metabolites associated with mitochondrial function on lifespan were investigated.

Malate, the tricarboxylic acid (TCA) cycle metabolite, increased lifespan and thermotolerance in C. elegans. Addition of fumarate and succinate also extended lifespan and all three metabolites activated nuclear translocation of the cytoprotective DAF-16/FOXO transcription factor and protected from paraquat-induced …

Mmv Malaria Box Activity Screening In Dormant Plasmodium Falciparum Phenotypes, Sandra Galusic

USF Tampa Graduate Theses and Dissertations

The causative agent of malignant tertian malaria, Plasmodium falciparum undergoes an arrested growth phenotype of its erythrocytic stage when under drug-stress. Recent artemisinin treatment failures seem to be indicative of such induction followed by recrudescence rather than actual therapeutic failure. Likewise, P. vivax hypnozoites are the prototypic dormants and the latent infections for which they are responsible prove most difficult to treat. Dihydroartemisinin, an artemisinin-derivative, can be used to exploit this mechanism by inducing a dormant state in ring-stage P. falciparum parasites and in turn, their recovery may be used as a screening period for compounds that inhibit or foster …

A Numerical And Experimental Investigation Of Flow Induced Noise In Hydraulic Counterbalance Valves, Mutasim Mohamed Elsheikh

USF Tampa Graduate Theses and Dissertations

The main objective of this study is to explore the complex fluid flow phenomena that result in the generation of a high frequency noise in counterbalance valves through an experimental and numerical investigation of the flow. Once the influence of the different components involved in noise generation is established, a secondary objective is the introduction of design modifications that eliminate the undesired effect without altering the operation envelope or the performance of the valve.

A hydraulic test bench was used to carry out an experimental investigation of the noise generation process. A computer based data acquisition system was used to …

Two Essays Examining Organizational Performance, Stacey Alexis Gelsheimer

USF Tampa Graduate Theses and Dissertations

In highly competitive industries where firms aren’t protected by barriers to entry and consumers’ preferences are constantly changing, the willingness and ability to adapt and continually improve operations may be critical for continued success. However, the internal exchanges between employees and managers that may be an integral part of the development and implementation of new ideas are often not modeled or included in our standard economic theories. In this research I investigate whether a higher level of employee voice (or employee input) predicts higher levels of continual improvement, and whether this continual improvement predicts higher levels of firm performance. Additionally, …

Community Identity And Social Practice During The Terminal Classic Period At Actuncan, Belize, Kara Ann Fulton

USF Tampa Graduate Theses and Dissertations

This research examines the relationship between the ways in which urban families engaged local landscapes and the development of shared identities at the prehispanic Maya city of Actuncan, Belize. Such shared identities would have created deep historical ties to specific urbanized spaces, which enabled and constrained political expansion during the Terminal Classic period (ca. A.D. 800–900), a time when the city experienced rapid population growth as surrounding centers declined. This research contributes to the understanding of urban processes of growth and decay in this region, and how they are linked to the behaviors of social factions in settlements.

For communities, …

Minocycline Treatment And The Necessity To Develop A Novel Outcome Measure For Children With Angelman Syndrome, Joseph Christopher Grieco

USF Tampa Graduate Theses and Dissertations

Angelman syndrome (AS) is a rare genetic disorder affecting 1/10,000 to 1/20,000 births. This disorder arises through the genetic disruption of the maternal UBE3A allele, which when coupled with epigenetic silencing of the paternal allele UBE3A allele, gives rise to an absence of UBE3A protein in the central nervous system. Clinical manifestations of the syndrome vary in severity and include poor motor function, deficits in language and severe intellectual impairments. Previous research in the Angelman syndrome mouse model revealed abnormalities in dendritic spine density and morphology of hippocampal pyramidal cells. As seen in humans with AS, mice show abnormal behavioral …

Immunological Characterization Of Duffy Binding Protein Of Plasmodium Vivax, Miriam Thankam George

USF Tampa Graduate Theses and Dissertations

Plasmodium vivax Duffy binding protein (DBP) is an essential ligand for reticulocyte invasion making it a premier asexual blood stage vaccine candidate. However, strain-specific immunity due to DBPII allelic variation may complicate vaccine efficacy, suggesting that an effective DBPII vaccine needs to target immune responses to conserved epitopes that are potential targets of strain-transcending neutralizing immunity. Anti DBPII monoclonal antibodies, which were previously characterized by COS7 cell binding assay as inhibitory and non-inhibitory to DBPII-erythrocyte binding, were mapped to DBPII gene fragment libraries using phage display. Inhibitory mAb 3C9 binds to a conserved conformation-dependent epitope in subdomain 3 while non-inhibitory …

Iwitness And Student Empathy: Perspectives From Usc Shoah Foundation Master Teachers, Brandon Jerome Haas

USF Tampa Graduate Theses and Dissertations

This qualitative interview study explores the perceptions of five USC Shoah Foundation Master Teachers who integrate IWitness in teaching about the Holocaust. The study focused on the perceptions of teachers as their students interact with survivor and witness testimony in IWitness as well as how IWitness provides a framework for moral education in comparison to other primary sources. Data gleaned from this study demonstrates the influence of personalized learning through testimony-based education on the development of empathy in secondary students. As IWitness is a new resource that engages students with Holocaust survivor testimony in innovative ways, this study fills a …

Muckraking And C.O.B.Y (Cry Of Black Youth): Uncovering A History Of Organizing In Belle Glade, Raymond A. Hamilton

USF Tampa Graduate Theses and Dissertations

This thesis examines a local activist group in the rural town of Belle Glade, Florida during the late 1960s and early 1970s. This research falls in line with many New Black Power studies. These New Black Power studies challenge existing notions of the Black Power and Civil Rights eras and their relationship to one another. It challenges the time frames, geography and ideology of both of the eras. This case study of a the group in Belle Glade is not the first to examine the similarities of the Black Power and Civil Rights eras, where many groups who affiliated with …

The Role Of The Interruption In Young Adult Epistolary Novels, Betty J. Herzhauser

USF Tampa Graduate Theses and Dissertations

Within the genre of young adult literature, a growing trend is the use of epistolary messages through electronic methods between characters. These messages are set apart from the formal text of the narrative of the novel creating a break in the text features and layout of the page. Epistolary texts require a more sophisticated reading method and level of interpretation because the epistolary style blends multiple voices and points of view into the plot, creating complicated narration. The reader must navigate the narrator’s path in order to extract meaning from the text. In this hermeneutic study, I examined the text …

Heart Of The Beholder: The Pathos, Truths And Narratives Of Thermopylae In _300_, James Christopher Holcom

USF Tampa Graduate Theses and Dissertations

This thesis argues that critical understanding of historical narratives needn’t be limited to cold, clinical applications of logic and reason. By doing a close textual reading of Frank Miller and Lynn Varley’s graphic novel, 300 and Zack Snyder’s 2007 film adaptation, I posit that critical analysis of popular narratives is better served when pathos takes a central role. Traditional rhetorical criticism tends to favor empirical evidence and fact over emotional, narrative truth. Yet, the writing, recounting and interpretation of history are more akin to arts than sciences. Historical narratives are subject to the same influences and techniques that make poetry, …

Tampa Electric Company's Big Bend Utility Plant In Hillsborough County, Florida: A Case Study, Lynne M. Hodalski-Champagne

USF Tampa Graduate Theses and Dissertations

This is an in-depth analysis of coal fire burning power plants, their effects on human health and the environment. It also employed case study data from Tampa Electric Company’s Big Bend facility to examine environmental infractions at that facility. Tampa Electric Company’s Big Bend Utility Plant, violated the Clean Air Act, which led to a lawsuit filed by the Department of Justice on behalf of the United States Environmental Protection Agency and the Florida Department of Environmental Protection in 1997. This case study details the lawsuit, and subsequent settlement as well as Tampa Electric Company’s record of compliance since 2000. …

Evidence Of Things Not Seen: A Semi-Automated Descriptive Phrase And Frame Analysis Of Texts About The Herbicide Agent Orange, Sarah Beth Hopton

USF Tampa Graduate Theses and Dissertations

From 1961 to 1971 the United States and the Republic of South Vietnam used chemicals to defoliate the coastal and upload forest areas of Viet Nam. The most notorious of these chemicals was named Agent Orange, a weaponized herbicide made up of two chemicals that, when combined, produced a toxic byproduct called TCDD-dioxin. Studied suggest that TCDD-dioxin causes significant human health problems in exposed American and Vietnamese veterans, and possibly their children (Agency, U.S. Environmental Protection, 2011). In the years since the end of the Vietnam War, volumes of discourse about Agent Orange has been generated, much of which is …

Comparison Of Isotope-Based Biomass Pathways With Groundfish Community Structure In The Eastern Gulf Of Mexico, Sheri Ann Huelster

USF Tampa Graduate Theses and Dissertations

This study compared traditional community analysis with stable-isotope trophic analysis to define process-based trophic elements of community structure in the eastern Gulf of Mexico, and developed a predictive capability regarding changes to fish community structure that would be expected from increasing eutrophication. Specifically, it used an existing trawl survey program (SEAMAP) to compare invertebrate herbivore (sponge and sea urchin) isotopes with groundfish isotopes, and then compared the resulting spatial patterns with spatial variation in community structure, as identified by cluster analysis. The comparison was applied to seven NMFS survey zones that extended offshore from the Caloosahatchee River, FL northwest to …

Equifinality And Multifinality In Psychopathology: Can Cognitive And Emotional Processes Differentiate Internalizing, Externalizing, And Co-Occurring Psychopathology, Brittany Jordan-Arthur

USF Tampa Graduate Theses and Dissertations

Despite our knowledge of environmental risk factors for psychopathology, the equifinality and multifinality observed in the extant literature reveals how little is known about the role of these risk factors in the development of psychopathology. The purpose of this study was to identify processes that differentiate internalizing, externalizing and co-occurring psychopathology. Specifically, emotion identification skill and cognitive appraisal style were examined as processes where individual differences may contribute to the development of mental illness. To date no study has been conducted to examine whether emotion identification and appraisal style may differentiate forms of internalizing, externalizing and co-occurring psychopathology and lack …

Tornado Fatalities: An In-Depth Look At Physical And Societal Influences, Heather Joann Key

USF Tampa Graduate Theses and Dissertations

The purpose of this study is to model and determine significant predictors of tornado death index values, and to investigate these significant predictors and what makes people vulnerable to tornado fatalities through expert interviews. This study also provides an understanding of the study participant’s perceptions of their county’s vulnerability to tornado fatality and demonstrates a true integration of methods and fields by studying geographic, meteorological, and sociological phenomena by use of quantitative and qualitative methods. The study consists of two parts: 1) A quantitative exploration of variables hypothesized to predict Tornado Death Index (TDI) values, 2) A qualitative investigation to …

System Threats And Gender Differences In Sexism And Gender Stereotypes, Sophie Lois Kuchynka

USF Tampa Graduate Theses and Dissertations

In the United States, women’s persistent gains in structural power may cause backlash among those motivated to preserve the status quo. The proposed study examines the conditions that prompt men and women to endorse sexism and promote gender stereotypes. System justification theory proposes that people are motivated to justify the socio-political system that governs them and threats to the stability of their system can increase individual’s motivated defenses. I expect men to show the strongest motivated defenses when the hierarchy is threatened or viewed as unstable, because to protect group-based interests men will reinforce the legitimacy of the system through …

The Role Of Anxiety Sensitivity In The Relationship Between Posttraumatic Stress Symptoms And Negative Outcomes In Trauma-Exposed Adults, Brittany Belle Kugler

USF Tampa Graduate Theses and Dissertations

Background: The development of posttraumatic stress symptoms (PTS) following a traumatic event is related to significant functional impairment, diminished quality of life, and physical health issues. Yet it is not entirely clear why some traumatized individuals experience negative outcomes while others do not. The purpose of this study is to determine the role of several influential factors related to PTS severity and negative outcomes, such as diminished quality of life and physical health issues, following traumatic events. Method: One hundred and twenty-two trauma-exposed adults were recruited through the University of South Florida’s SONA system and through flyers on campus. Subjects …

How You Categorize Influences How Helpful You Are: The Effect Of Categorization Mindset On Consumers’ Social Decisions, Hsiao-Ching Kuo

USF Tampa Graduate Theses and Dissertations

This dissertation demonstrates how categorization mindsets (introduced by Ulkumen et al., 2010) moderate the altruistic behavior of consumers in decisions that have consequences to others besides oneself. Categorization mindset refers to a way of thinking about options, and is induced by simple sorting or categorization tasks. Ulkumen et al. (2010) has shown that mindsets can be unidimensional (in terms of being focused on a single, salient dimension) or multidimensional (in that both salient and non-salient dimensions are processed). Across three experiments, this dissertation finds that a multidimensional mindset (compared to a unidimensional mindset) enhances the preference for other-oriented options among …

Schreibersite: Synthesis, Characterization And Corrosion And Possible Implications For Origin Of Life, Nikita Latesha La Cruz

USF Tampa Graduate Theses and Dissertations

We present study of the synthesis and reactions of an analog of the meteoritic mineral schreibersite with formula (Fe,Ni)3P, believed to be a prebiotic source of reactive phosphorus that may have prompted the formation of phosphorylated biomolecules near the time of the origin of life (Pasek and Lauretta, 2005). The mineral was synthesized by mixing stoichiometric proportions of elemental iron, nickel and phosphorus and heating in a tube furnace at 820°C for approximately 235 hours under argon or under vacuum, a modification of the method of Skála and Drábek (2002). The mineral was characterized using X-ray diffractometry (XRD), X-ray photoelectron …

Experimental Reporting And Networks Of Political Information: Lorenzo Magalotti's Framing Of Courts And Nature, Bradley L'Herrou

USF Tampa Graduate Theses and Dissertations

This thesis explores changes in experimental reporting during the scientific revolution of the seventeenth century. In particular, I examine and compare some of the works of Count Lorenzo Magalotti, namely the Saggi di Naturali Esperienza or Essays on Natural Experiments and the Relazione d'Inghilterra. In 1667, as secretary of the Accademia del Cimento – the Tuscan experimental academy founded in 1657 – Magalotti (1637-1712) authored the Saggi, a collection of experimental reports. These reports included extensive written descriptions of experiments along with dozens of engravings depicting the instruments custom-made for the experiments. Magalotti also served as ambassador and agent of …

Historical And Current Population Patterns Of The Staghorn Coral (_Acropora Cervicornis_) In Dry Tortugas National Park, Kaitlyn Lizza

USF Tampa Graduate Theses and Dissertations

Acropora cervicornis was once one of the dominant reef building corals of the Caribbean, Florida Keys, and Dry Tortugas (DRTO), but since the 1970’s populations have been decimated throughout their geographic range. Recently, a repopulation was documented through detailed benthic surveys conducted by the U.S. Geological Survey at three locations (Pulaski Shoal, East Key, and Loggerhead Key) within DRTO. Benthic surveys using the U.S Geological Survey’s Along-Track Reef Imaging System (ATRIS) revealed hundreds of previously undocumented colonies.

These discoveries have provided a unique data-set, allowing a comparison between the historical (1883, 1976) and contemporary distributions (2009, 2011) of A. cervicornis. …

'She Shall Not Be Moved': Black Women's Spiritual Practice In Toni Morrison's The Bluest Eye, Beloved, Paradise, And Home, Rondrea Danielle Mathis

USF Tampa Graduate Theses and Dissertations

‘She Shall Not Be Moved’: Black Women’s Spiritual Practice in Toni Morrison’s The Bluest Eye, Beloved, Paradise, and Home argues that from The Bluest Eye, Morrison’s debut novel, to her 2012 novel, Home, Morrison brings her female characters to voice, autonomy, and personal divinity through unconventional spiritual work. The project addresses the history of Black women’s activist and spiritual work, Toni Morrison’s engagement with unconventional spiritual practice, and closes with a personal interrogation of the author’s connection to Black women’s spiritual practice.

Beauty Is Precious, Knowledge Is Power, And Innovation Is Progress: Widely Held Beliefs In Policy Narratives About Oil Spills, Brenda Gale Mason

USF Tampa Graduate Theses and Dissertations

Scholars from diverse perspectives have sought to understand the features and mechanisms that influence the design and implementation of public policy. Some (realists) have emphasized the role that material interests have played while others (idealists) have emphasized the influence of subjective ideas on ‘how policy means’ (Yanow 1996). Recently, observers in both camps have demonstrated curiosity in the influence of culture on policymaking and its consequences. Regrettably, this shared concern has not resulted in much collaboration across epistemological divides.

I argue that narrative analysis provides a way to bridge the divides by specifying an interpretive approach that identifies culture as …

Documenting Perceived Effectiveness Of Community-Based Health Promotion Coalitions: A Grounded Theory Approach, Alyssa Brooke Mayer

USF Tampa Graduate Theses and Dissertations

Introduction: Community coalitions with public health-related missions are formal, semi-permanent, action-oriented partnerships comprised of community members, representatives of government agencies, policymakers, and academic partners. Despite their potential to promote sustainable change, coalitions have had mixed success in effecting long-term improvements in community health. There is a need to assist them in developing strategies for improving and sustaining their functionality. The purpose of this study was to improve understanding of the elements of coalition success and sustainability that are vital to health-related community-based participatory research.

Methods: Although the literature describes coalition functions for effecting sustainable programs and policies, most research reports …

Effects Of A Brief Relaxation Intervention On Stress-Related Eating, Laura Lynn Mayhew-Purcell

USF Tampa Graduate Theses and Dissertations

The experience of stress may contribute to increased food consumption and selection of unhealthy food options. Resource depletion theory suggests stress temporarily depletes resources needed to regulate behavior. Depletions of self-control may result in subsequent failure to regulate eating behaviors, which is particularly salient in restrained eaters. Restraint theory posits people high in dietary restraint require significant effort to control eating. Emotional eating theory further suggests palatable foods may be used to regulate emotional stress reactions. Relaxation exercises to mitigate stress reactions are recommended in eating and weight management programs, but lack quality scientific support. The current study examined the …