Digital Commons Network^™

Straight Benevolence: Preserving Heterosexual Authority And White Privilege, Robb James Bruce

USF Tampa Graduate Theses and Dissertations

This thesis analyzes three current and popular media sites, exploring a term I coin “straight benevolence.” An ostensibly supportive and progressive attitude adopted by heterosexuals and expressed toward gay men in the United States, straight benevolence surreptitiously subordinates gayness and further entrenches white masculine heterosexual privilege. In my examination of hip-hop artist Macklemore’s “Same Love,” seven Major League Baseball “It Gets Better” gay-advocacy videos, and the “Suddenly, Last Summer” episode of ABC’s primetime U.S. television series Modern Family, I take an intersectional approach to address the interanimation of sexuality, gender, and race. I ask: In what ways is gay male …

Functional Analysis Of The Ovarian Cancer Susceptibility Locus At 9p22.2 Reveals A Transcription Regulatory Network Mediated By Bnc2 In Ovarian Cells, Melissa Buckley

USF Tampa Graduate Theses and Dissertations

GWAS have identified several chromosomal loci associated with ovarian cancer risk. However, the mechanism underlying these associations remains elusive. We identify candidate functional Single Nucleotide Polymorphisms (SNPs) at the 9p22.2 ovarian cancer susceptibility locus, several of which map to transcriptional regulatory elements active in ovarian cells identified by FAIRE-seq (Formaldehyde assisted isolation of regulatory elements followed by sequencing) and ChIP-seq (Chromatin Immunoprecipitation followed by sequencing) in relevant cell types. Reporter and electrophoretic mobility shift assays (EMSA) determined the extent to which candidate SNPs had allele specific effects. Chromosome conformation capture (3C) reveals a physical association between Basonuclin 2 (BNC2) and …

The Relation Between Adiposity And Anxiety In Youth: Analysis Of Peer Victimization, Teasing, Sociocultural Influences, And Internalization Of Appearance Ideals As Explanatory Variables, Natasha L. Burke

USF Tampa Graduate Theses and Dissertations

The purpose of the current study was to examine putative mediators and moderators in the association between adiposity and anxiety in a sample of overweight and obese youth. In addition, anxiety was examined as a potential moderator between adiposity and health-related quality of life (HRQOL). Participants were youth (N = 137) between 8 and 17 years old (M = 13.09, SD = 2.61) and their legal caregivers recruited from four medical clinics affiliated with the University of South Florida. Youth were primarily overweight (28.5%) or obese (64.2%) and ethnically diverse. Data were analyzed by path analysis. Weight-related teasing significantly mediated …

Self-Assembly Kinetics Of Microscale Components: A Parametric Evaluation, Jose Miguel Carballo

USF Tampa Graduate Theses and Dissertations

The goal of the present work is to develop, and evaluate a parametric model of a basic microscale Self-Assembly (SA) interaction that provides scaling predictions of process rates as a function of key process variables. At the microscale, assembly by “grasp and release” is generally challenging. Recent research efforts have proposed adapting nanoscale self-assembly (SA) processes to the microscale. SA offers the potential for reduced equipment cost and increased throughput by harnessing attractive forces (most commonly, capillary) to spontaneously assemble components. However, there are challenges for implementing microscale SA as a commercial process. The existing lack of design tools prevents …

Robust, Low Power, Discrete Gate Sizing, Anthony Joseph Casagrande

USF Tampa Graduate Theses and Dissertations

Ultra-deep submicron circuits require accurate modeling of gate delay in order to meetaggressive timing constraints. With the lack of statistical data, variability due to the mechanical manufacturing process and its chemical properties poses a challenging problem. Discrete gate sizing requires (i) accurate models that take into account random parametric variation and (ii) a fair allocation of resources to optimize the solution. The proposed GTFUZZ gate sizing algorithm handles both tasks. Gate sizing is modeled as a resource allocation problem using fuzzy game theory. Delay is modeled as a constraint and power is optimized in this algorithm. In GTFUZZ, delay is …

Exploring The Pathogenic And Drug Resistance Mechanisms Of Staphylococcus Aureus, Whittney Burda

USF Tampa Graduate Theses and Dissertations

We have previously identified σS, an ECF sigma factor that is important in the virulence and stress response of S. aureus. Transcriptional profiling of sigS revealed that it is differentially regulated in a variety of laboratory and clinical strains of S. aureus, suggesting that there exists a regulatory network that modulates its expression. In order to identify direct regulators of sigS expression, we performed a biotin pull down assay in tandem with mass spectrometry. We identified CymR as a direct regulator and observed that sigS expression is increased in cells lacking cymR. In addition, transposon mutagenesis was performed to identify …

Latino Immigrant Workers’ Search For Justice After Occupational Injury, Carla Gabriela Castillo

USF Tampa Graduate Theses and Dissertations

Latino immigrants encounter an entanglement of rights and policies after occupational injury or illness. In collaboration with an immigrant worker center, ethnographic research and a survey are used to analyze injured workers’ experiences. The center uses survey results to identify common threads and systematic problems, and to explore potential direct action. Through interviews with workers and medical and legal professionals, I investigate the barriers Latino immigrants face following occupational injury or illness, how their lived experiences relate to the greater medicolegal frameworks that demarcate most formal processes of compensation and treatment, and the experiences of professionals who mediate these structures. …

Crisis Communication And Celebrity Scandal: An Experiment On Response Strategies, Leah Champion

USF Tampa Graduate Theses and Dissertations

Recent allegations surrounding Bill Cosby presented an opportunity to combine, test, and extend situational crisis communication theory typology and image repair theory response strategies for celebrity use. Because Cosby did not respond to the numerous allegations against him, it presented an opportunity to experiment with new and existing typologies using a real case, as opposed to analyzing past response attempts or using a hypothetical case. This study used a 2 X 4 factorial design to test veracity of claims, a proposed concept, and response strategies via survey. Even though none of the hypotheses were supported, the present study opened up …

Effects Of Occupational Stressors On Nurses’ Safety Performance And Well-Being: A Within-Individual Study, Xinxuan Che

USF Tampa Graduate Theses and Dissertations

Occupational stressors have been extensively studied as predictors of safety performance and employee well-being in previous research. However, many newly introduced organizational constructs that have the characteristics of an occupational stressor have rarely been studied as such, especially from a within-person perspective. The current study focused on three occupational stressors in relation to safety performance. Based on previous literature, I proposed that within individuals, compulsory citizenship behavior, illegitimate tasks, and interpersonal conflict at work as occupational stressors would have negative effects on employees well-being and safety performance through negative emotions (anger), job attitudes (job satisfaction and organizational commitment) and role …

Script Training: The Role Of Written Cues, Hallie Cohen

USF Tampa Graduate Theses and Dissertations

Script training is a technique that allows persons with acquired speech and language disorders, such as nonfluent aphasia, to have islands of fluent speech during which they can speak about a topic without pausing or having word-finding errors. Scripts relevant to specific functional situations are written and practiced until memorized. Script training delivered verbally has been effective with clients with aphasia but the role of written cues in the training has not been explored. Therefore the purpose of this study was to compare the effectiveness of script training taught verbally, or verbally with a written script, in persons with aphasia. …

Computational Investigations Of Potential Energy Function Development For Metal-Organic Framework Simulations, Metal Carbenes, And Chemical Warfare Agents, Christian R. Cioce

USF Tampa Graduate Theses and Dissertations

Metal-Organic Frameworks (MOFs) are three-dimensional porous nanomaterials with a variety of applications, including catalysis, gas storage and separation, and sustainable energy. Their potential as air filtration systems is of interest for designer carbon capture materials. The chemical constituents (i.e. organic ligands) can be functionalized to create rationally designed CO2 sequestration platforms, for example. Hardware and software alike at the bleeding edge of supercomputing are utilized for designing first principles-based molecular models for the simulation of gas sorption in these frameworks. The classical potentials developed herein are named PHAST -- Potentials with High Accuracy, Speed, and Transferability, and thus are designed …

High-Throughput Screening Of Age-Related Changes In Caenorhabditis Elegans, Neil Copes

USF Tampa Graduate Theses and Dissertations

This project was developed to identify novel methods for high-throughput culturing and screening of C. elegans to investigate age-related metabolic changes and to survey the proteomic and metabolomic factors associated with age-related changes. To accomplish these goals we developed a novel way to grow C. elegans in liquid culture in 96-well microplates for several weeks without suffering significant fluid loss due to evaporation and without needing to shake or unseal the plates for aeration. We also developed methods for assaying the total volume of live C. elegans in microplate cultures using a fluorescence microplate reader and for performing RNAi experiments …

The “Other” Side Of Wall Street: Banking, Policies, And Adaptive Methods Of U.S. Migrant Workers, Cassandra Rae Decker

USF Tampa Graduate Theses and Dissertations

Migrant farmworkers' social and economic mobility is frequently constrained through the denial of basic resources, such as access to the formal financial sector. This thesis ethnographically examines banking policies as they apply to low-income, mobile, populations that temporarily reside in Florida. It utilizes participant observation, interviews, and participatory mapping with migrant farmworkers. It also considers how policymakers and service providers in the formal and informal financial sectors rationalize control of resources and the effects on mobile populations. Particular attention is paid to adaptive practices in the alternative financial sector – cash checking services, carrying cash, and remittances. By utilizing the …

Strategies For Preventing Age And Neurodegenerative Disease-Associated Mitochondrial Dysfunction, Vedad Delic

USF Tampa Graduate Theses and Dissertations

Mitochondrial dysfunction plays a pivotal role in the development of aging phenotypes and aging-associated neurodegenerative disorders, such as Alzheimer’s disease (AD), Parkinson’s disease (PD) and Amyotrophic lateral sclerosis (ALS). Strategies that restore mitochondrial dysfunction may rescue the deficits of central metabolism in these disorders and improve cell survival. For example, we found that modulating the mTOR signaling pathway in a tissue culture model of aging-induced mitochondrial DNA mutation enhanced mitochondrial function as evidenced by increased oxygen consumption. Our previous melatonin studies also led us to hypothesize that caloric restriction and the hormone melatonin would reverse brain mitochondrial dysfunction in animal …

The Continuum Of Ethno-Racial Socialization: Learning About Culture And Race In Middle-Class Latina/O Families, Maria D. Duenas

USF Tampa Graduate Theses and Dissertations

This thesis examines the discursive messages and specific practices that Latino families use to transmit messages about culture, race, and racism. Scholars have not fully explored the complexity and range of practices and discourses that are involved in Latinos’ ethno-racial socialization. The use of the phrase “ethno-racial socialization” is important because it combines the concepts of racial socialization and ethnic socialization in an effort to account for how the lived experiences of Latinos who mostly think of themselves as a racial group, are treated as one race, and thus, discuss race with family members. This research explores this process using …

Compassion Fatigue And Self-Care Strategies Among Addiction Professionals: A Qualitative Study, Amanda Depippo

USF Tampa Graduate Theses and Dissertations

Addiction is a prevalent disorder and is on the rise. Addiction has serious symptomology and can negatively impact an individual’s life in several areas. Not only can the person addicted become negatively impacted, but the addiction professional that treats this disorder can become negatively impacted as well. Because there is often a high co-occurrence of addiction and trauma among addiction clients, this intensifies the clinical profile of such clients which compounds the risk for addiction professionals. It is the addiction professional’s response to this combination of addiction and trauma that can cause these professionals to experience compassion fatigue.

Research has …

Genomic Aberrations At The 3q And 14q Loci: Investigation Of Key Players In Ovarian And Renal Cancer Biology, Punashi Dutta

USF Tampa Graduate Theses and Dissertations

Genomic aberrations are primary contributors to the pathophysiology of cancer [11]. Dysregulated expression of genes located within these aberrations are important predictors of chemoresistance, disease prognosis, and patient outcome [12]. This dissertation is focused on understanding the regulation and/or functions of specific genes located at dysregulated genomic regions such as 3q26 and 14q32 in the biology of ovarian and renal cancer, respectively.

Serous epithelial ovarian cancer (EOC) manifest amplification at the 3q26.2 locus [2], an observation consistent with the cancer genome atlas (TCGA) [13]. The most amplified gene in this region is EVI1 which has been extensively studied in hematological …

The Effects Of Supplemented Metabolites On Lifespan And Stress Response Pathways In Caenorhabditis Elegans, Clare B. Edwards

USF Tampa Graduate Theses and Dissertations

Understanding how metabolites contribute to anaplerosis, antioxidant effects, and hormetic pathways during aging is fundamental to creating supplements and dietary habits that may decrease age-associated disease and decline, thus improving the quality of life in old age. In order to uncover metabolic pathways that delay aging, the effects of large sets of metabolites associated with mitochondrial function on lifespan were investigated.

Malate, the tricarboxylic acid (TCA) cycle metabolite, increased lifespan and thermotolerance in C. elegans. Addition of fumarate and succinate also extended lifespan and all three metabolites activated nuclear translocation of the cytoprotective DAF-16/FOXO transcription factor and protected from paraquat-induced …

Mmv Malaria Box Activity Screening In Dormant Plasmodium Falciparum Phenotypes, Sandra Galusic

USF Tampa Graduate Theses and Dissertations

The causative agent of malignant tertian malaria, Plasmodium falciparum undergoes an arrested growth phenotype of its erythrocytic stage when under drug-stress. Recent artemisinin treatment failures seem to be indicative of such induction followed by recrudescence rather than actual therapeutic failure. Likewise, P. vivax hypnozoites are the prototypic dormants and the latent infections for which they are responsible prove most difficult to treat. Dihydroartemisinin, an artemisinin-derivative, can be used to exploit this mechanism by inducing a dormant state in ring-stage P. falciparum parasites and in turn, their recovery may be used as a screening period for compounds that inhibit or foster …

A Numerical And Experimental Investigation Of Flow Induced Noise In Hydraulic Counterbalance Valves, Mutasim Mohamed Elsheikh

USF Tampa Graduate Theses and Dissertations

The main objective of this study is to explore the complex fluid flow phenomena that result in the generation of a high frequency noise in counterbalance valves through an experimental and numerical investigation of the flow. Once the influence of the different components involved in noise generation is established, a secondary objective is the introduction of design modifications that eliminate the undesired effect without altering the operation envelope or the performance of the valve.

A hydraulic test bench was used to carry out an experimental investigation of the noise generation process. A computer based data acquisition system was used to …

Two Essays Examining Organizational Performance, Stacey Alexis Gelsheimer

USF Tampa Graduate Theses and Dissertations

In highly competitive industries where firms aren’t protected by barriers to entry and consumers’ preferences are constantly changing, the willingness and ability to adapt and continually improve operations may be critical for continued success. However, the internal exchanges between employees and managers that may be an integral part of the development and implementation of new ideas are often not modeled or included in our standard economic theories. In this research I investigate whether a higher level of employee voice (or employee input) predicts higher levels of continual improvement, and whether this continual improvement predicts higher levels of firm performance. Additionally, …

Community Identity And Social Practice During The Terminal Classic Period At Actuncan, Belize, Kara Ann Fulton

USF Tampa Graduate Theses and Dissertations

This research examines the relationship between the ways in which urban families engaged local landscapes and the development of shared identities at the prehispanic Maya city of Actuncan, Belize. Such shared identities would have created deep historical ties to specific urbanized spaces, which enabled and constrained political expansion during the Terminal Classic period (ca. A.D. 800–900), a time when the city experienced rapid population growth as surrounding centers declined. This research contributes to the understanding of urban processes of growth and decay in this region, and how they are linked to the behaviors of social factions in settlements.

For communities, …

Minocycline Treatment And The Necessity To Develop A Novel Outcome Measure For Children With Angelman Syndrome, Joseph Christopher Grieco

USF Tampa Graduate Theses and Dissertations

Angelman syndrome (AS) is a rare genetic disorder affecting 1/10,000 to 1/20,000 births. This disorder arises through the genetic disruption of the maternal UBE3A allele, which when coupled with epigenetic silencing of the paternal allele UBE3A allele, gives rise to an absence of UBE3A protein in the central nervous system. Clinical manifestations of the syndrome vary in severity and include poor motor function, deficits in language and severe intellectual impairments. Previous research in the Angelman syndrome mouse model revealed abnormalities in dendritic spine density and morphology of hippocampal pyramidal cells. As seen in humans with AS, mice show abnormal behavioral …

Immunological Characterization Of Duffy Binding Protein Of Plasmodium Vivax, Miriam Thankam George

USF Tampa Graduate Theses and Dissertations

Plasmodium vivax Duffy binding protein (DBP) is an essential ligand for reticulocyte invasion making it a premier asexual blood stage vaccine candidate. However, strain-specific immunity due to DBPII allelic variation may complicate vaccine efficacy, suggesting that an effective DBPII vaccine needs to target immune responses to conserved epitopes that are potential targets of strain-transcending neutralizing immunity. Anti DBPII monoclonal antibodies, which were previously characterized by COS7 cell binding assay as inhibitory and non-inhibitory to DBPII-erythrocyte binding, were mapped to DBPII gene fragment libraries using phage display. Inhibitory mAb 3C9 binds to a conserved conformation-dependent epitope in subdomain 3 while non-inhibitory …

Iwitness And Student Empathy: Perspectives From Usc Shoah Foundation Master Teachers, Brandon Jerome Haas

USF Tampa Graduate Theses and Dissertations

This qualitative interview study explores the perceptions of five USC Shoah Foundation Master Teachers who integrate IWitness in teaching about the Holocaust. The study focused on the perceptions of teachers as their students interact with survivor and witness testimony in IWitness as well as how IWitness provides a framework for moral education in comparison to other primary sources. Data gleaned from this study demonstrates the influence of personalized learning through testimony-based education on the development of empathy in secondary students. As IWitness is a new resource that engages students with Holocaust survivor testimony in innovative ways, this study fills a …

Muckraking And C.O.B.Y (Cry Of Black Youth): Uncovering A History Of Organizing In Belle Glade, Raymond A. Hamilton

USF Tampa Graduate Theses and Dissertations

This thesis examines a local activist group in the rural town of Belle Glade, Florida during the late 1960s and early 1970s. This research falls in line with many New Black Power studies. These New Black Power studies challenge existing notions of the Black Power and Civil Rights eras and their relationship to one another. It challenges the time frames, geography and ideology of both of the eras. This case study of a the group in Belle Glade is not the first to examine the similarities of the Black Power and Civil Rights eras, where many groups who affiliated with …

The Role Of The Interruption In Young Adult Epistolary Novels, Betty J. Herzhauser

USF Tampa Graduate Theses and Dissertations

Within the genre of young adult literature, a growing trend is the use of epistolary messages through electronic methods between characters. These messages are set apart from the formal text of the narrative of the novel creating a break in the text features and layout of the page. Epistolary texts require a more sophisticated reading method and level of interpretation because the epistolary style blends multiple voices and points of view into the plot, creating complicated narration. The reader must navigate the narrator’s path in order to extract meaning from the text. In this hermeneutic study, I examined the text …

Heart Of The Beholder: The Pathos, Truths And Narratives Of Thermopylae In _300_, James Christopher Holcom

USF Tampa Graduate Theses and Dissertations

This thesis argues that critical understanding of historical narratives needn’t be limited to cold, clinical applications of logic and reason. By doing a close textual reading of Frank Miller and Lynn Varley’s graphic novel, 300 and Zack Snyder’s 2007 film adaptation, I posit that critical analysis of popular narratives is better served when pathos takes a central role. Traditional rhetorical criticism tends to favor empirical evidence and fact over emotional, narrative truth. Yet, the writing, recounting and interpretation of history are more akin to arts than sciences. Historical narratives are subject to the same influences and techniques that make poetry, …

Tampa Electric Company's Big Bend Utility Plant In Hillsborough County, Florida: A Case Study, Lynne M. Hodalski-Champagne

USF Tampa Graduate Theses and Dissertations

This is an in-depth analysis of coal fire burning power plants, their effects on human health and the environment. It also employed case study data from Tampa Electric Company’s Big Bend facility to examine environmental infractions at that facility. Tampa Electric Company’s Big Bend Utility Plant, violated the Clean Air Act, which led to a lawsuit filed by the Department of Justice on behalf of the United States Environmental Protection Agency and the Florida Department of Environmental Protection in 1997. This case study details the lawsuit, and subsequent settlement as well as Tampa Electric Company’s record of compliance since 2000. …

Evidence Of Things Not Seen: A Semi-Automated Descriptive Phrase And Frame Analysis Of Texts About The Herbicide Agent Orange, Sarah Beth Hopton

USF Tampa Graduate Theses and Dissertations

From 1961 to 1971 the United States and the Republic of South Vietnam used chemicals to defoliate the coastal and upload forest areas of Viet Nam. The most notorious of these chemicals was named Agent Orange, a weaponized herbicide made up of two chemicals that, when combined, produced a toxic byproduct called TCDD-dioxin. Studied suggest that TCDD-dioxin causes significant human health problems in exposed American and Vietnamese veterans, and possibly their children (Agency, U.S. Environmental Protection, 2011). In the years since the end of the Vietnam War, volumes of discourse about Agent Orange has been generated, much of which is …