Digital Commons Network^™

Iron Toxicity In The Retina Requires Alu Rna And The Nlrp3 Inflammasome, Bradley D. Gelfand, Charles B. Wright, Younghee Kim, Tetsuhiro Yasuma, Reo Yasuma, Shengjian Li, Benjamin J. Fowler, Ana Bastos-Carvalho, Nagaraj Kerur, Annette Uittenbogaard, Youn Seon Han, Dingyuan Lou, Mark E. Kleinman, W. Hayes Mcdonald, Gabriel Núñez, Philippe Georgel, Joshua L. Dunaief, Jayakrishna Ambati

Ophthalmology and Visual Science Faculty Publications

Excess iron induces tissue damage and is implicated in age-related macular degeneration (AMD). Iron toxicity is widely attributed to hydroxyl radical formation through Fenton's reaction. We report that excess iron, but not other Fenton catalytic metals, induces activation of the NLRP3 inflammasome, a pathway also implicated in AMD. Additionally, iron-induced degeneration of the retinal pigmented epithelium (RPE) is suppressed in mice lacking inflammasome components caspase-1/11 or Nlrp3 or by inhibition of caspase-1. Iron overload increases abundance of RNAs transcribed from short interspersed nuclear elements (SINEs): Alu RNAs and the rodent equivalent B1 and B2 RNAs, which are inflammasome agonists. Targeting …

Oral History Of Student Life At The Uk College Of Law With Thomas Givhan (Class Of 1951) | Part 2, Thomas Givhan

Kentucky Law Alumni Oral History Collection

The downloadable document is a transcript of the oral history in PDF format. Below, you will find a streaming media file to hear the oral history.

This oral history is part of the Kentucky Law Alumni Oral History Collection in UKnowledge. Thomas Givhan (Class of 1951) is interviewed by Professor Franklin Runge about his experiences at the UK College of Law.

Oral History Of Student Life At The Uk College Of Law With Thomas Givhan (Class Of 1951) | Part 3, Thomas Givhan

Kentucky Law Alumni Oral History Collection

The downloadable document is a transcript of the oral history in PDF format. Below, you will find a streaming media file to hear the oral history.

This oral history is part of the Kentucky Law Alumni Oral History Collection in UKnowledge. Thomas Givhan (Class of 1951) is interviewed by Professor Franklin Runge about his experiences at the UK College of Law.

Cancer Platinum Resistance Detection And Sensitization Method, Johnathan Lancaster, Douglas C. Marchion, Dung-Tsa Chen

USF Patents

The phosphorylation status of the BAD protein is a determinant of ovarian cancer cell responsiveness to platinum chemotherapy. Indirect manipulation of BAD phosphorylation status influences cisplatin sensitivity. BAD phosphorylation represents a biomarker that predicts platinum sensitivity and is a therapeutic target to increase platinum sensitivity. The methods employ phospho-specific antibody against a particular amino acid residue or site. Phospho-specific protein characterization methods include immunohistochemical (IHC), flow cytometric, immunofluorescent, capture-and-detection, or reversed phase assay.

Heidegger's Gods: An Ecofeminist Perspective, Susanne Claxton

Philosophy ETDs

The aim of this dissertation is to address the importance and significance of Heidegger's engagement with the Greeks and the ways in which his views are commensurate with ecofeminism and the insights that a study of that intersection provides. I defend the thesis that a proper return to myth and art as a means by which the transcendental realities that constitute the phenomenology of our embodied existence may be better understood is what may allow us to truly dwell in the Heideggerian sense and live full lives rich in meaning and value. My methodology might best be described as a …

The Sox9 Upstream Region Prone To Chromosomal Aberrations Causing Campomelic Dysplasia Contains Multiple Cartilage Enhancers, Baojin Yao, Qiuqing Wang, Chia-Feng Liu, Pallavi Bhattaram, Wei Li, Timothy J. Mead, James F. Crish, Véronique Lefebvre

Biomedical Sciences

Two decades after the discovery that heterozygous mutations within and around SOX9 cause campomelic dysplasia, a generalized skeleton malformation syndrome, it is well established that SOX9 is a master transcription factor in chondrocytes. In contrast, the mechanisms whereby translocations in the –350/–50-kb region 5 of SOX9 cause severe disease and whereby SOX9 expression is specified in chondrocytes remain scarcely known. We here screen this upstream region and uncover multiple enhancers that activate Sox9-promoter transgenes in the SOX9 expression domain. Three of them are primarily active in chondrocytes. E250 (located at – 250 kb) confines its activity to condensed prechondrocytes, …

The Eastern Echo, June 23, 2015, Eastern Michigan University

EMU Student Newspaper: The Normal News & The Eastern Echo

No abstract provided.

The Murray Ledger And Times, June 23, 2015, The Murray Ledger And Times

The Murray Ledger & Times

No abstract provided.

Board Of Trustee Members Approve Faculty Changes, Winthrop University

Winthrop News 2015

• The Division of Academic Affairs presented the names of the faculty members who earned tenure or promotion after an extensive review process this year.
• Nine faculty members were granted sabbaticals, and another seven retired with emeritus status.

Nonparametric Bayes Approach For A Semi-Mechanistic Pharmacokinetic And Pharmacodynamic Model, Yan Dong

Mathematics & Statistics ETDs

Both frequentist and Bayesian approaches have been used to characterize population pharmacokinetics and pharmacodynamics(PK/PD) models. These methods focus on estimating the population parameters and assessing the association between the characteristics of PK/PD and the subject covariates. In this work, we propose a Dirichlet process mixture model to classify the patients based on their individualized pharmacokinetic and pharmacodynamic profiles. Then we can predict the new patients dose-response curves given their concentration-time profiles. Additionally, we implement a modern Markov Chain Monte Carlo algorithm for sampling inference of parameters. The detailed sampling procedures as well as the results are discussed in a simulation …

Cells Too Stiff To Scavenge Leads To Lupus, An Autoimmune Disease, Jeff Hansen

Browse All News

No abstract provided.

Biofilm Accumulation And Immune Response During Experimental Peri-Implant Mucositis., Amy Rosania

Master's Theses

No abstract provided.

Shell Neurons Of The Master Circadian Clock Coordinate The Phase Of Tissue Clocks Throughout The Brain And Body, Jennifer A. Evans, Ting-Chung Suen, Ben L. Callif, Andrew S. Mitchell, Oscar Castanon-Cervantes, Kimberly M. Baker, Ian Kloehn, Kenkichi Baba, Brett J.W. Teubner, J. Christopher Ehlen, Ketema N. Paul, Timothy J. Bartness, Gianluca Tosini, Tanya Leise, Alec J. Davidson

Biological Sciences Faculty Research and Publications

Background: Daily rhythms in mammals are programmed by a master clock in the suprachiasmatic nucleus (SCN). The SCN contains two main compartments (shell and core), but the role of each region in system-level coordination remains ill defined. Herein, we use a functional assay to investigate how downstream tissues interpret region-specific outputs by using in vivo exposure to long day photoperiods to temporally dissociate the SCN. We then analyze resulting changes in the rhythms of clocks located throughout the brain and body to examine whether they maintain phase synchrony with the SCN shell or core. Results: Nearly all of the 17 …

Thicker Than Water: America’S Addiction To Cheap Flood Insurance, Jeffrey Valacer

Pace Law Review

This paper is broken down into three parts. Part I traces the history and evolution of flood insurance in the United States, including the establishment of federal flood insurance and key reforms over the 20th and 21st centuries. Part II discusses the 2012 flood insurance reform package, subsequent legal challenges to the reforms, and the government’s response to political pressure over the reform. Part III concludes discussing the continued need for flood insurance reform, especially in a world of rising sea levels and more frequent, stronger weather events.

Begone, Euclid!: Leasing Custom And Zoning Provision Engaging Retail Consumer Tastes And Technologies In Thriving Urban Centers, Michael N. Widener

Pace Law Review

Is urban center retailing in a death spiral? Competition for consumers with Internet vendors is afoot; winners and losers shall be anointed. The threats to physical retailing in an era of the “Internet of Goods” initially are described below. Adaptations by tenants, landlords, and stakeholders in urban centers will be required quickly, and new perspectives and partnerships, including those among local and regional governments, are instrumental if physical retail operations in municipal cores are to survive. The balance of this article describes these needs from the vantage point of each stakeholder; but this article argues that integrating information and communication …

Gsu View, 2015-06-22, Office Of Marketing And Communications

GSU View

Newsletter published by Governors State University 2007-current.

Understanding The Sympathetic Nervous System In Response To Food In Children With Autism And Typically Developing Children, Jonathon D. Haskell

Occupational Therapy Graduate Student Evidenced-Based Research Reviews

Previous research behind sympathetic nervous system functioning in children with Autism Spectrum Disorder has shown disturbances in their ability to regulate their arousal level (Miller et al., 2003; Tomcheck and Dunn, 2000). Depicting this notion in previous research, children diagnosed with autism are likely to be comorbid with Sensory Over-responsitivity (SOR) or Sensory under-responsitivity (SUR), and could ultimately elicit variable sympathetic nervous system functioning in response to their sensory environment-whether it be taste, smell, auditory, tactile, or visual stimuli. Although previous research depicts inconsistent measures involving sympathetic responses to stimuli in children with autism, research specifically involving food selectivity provided …

Current, June 22, 2015, University Of Missouri-St. Louis

Current (2010s)

No abstract provided.

Effect Of Social Skill Training Programs On Bullying, Kaycee Johns, Megan Kloska

Occupational Therapy Graduate Student Evidenced-Based Research Reviews

Bullying is a problem that is often found in schools during the adolescence age. Although this is not a new concept, bullying continues to lead to the victimization of children and adolescents all over the world. Research has played a critical role in understanding bullying, victimization, and prevention programs. However, bullying and victimization still continue to be problems within schools and peer relationships.

The primary purpose of this systematic review is to assist practitioners by investigating evidence-based studies related to school social skill programs, and their effectiveness in reducing victimization of bullying throughout adolescence.

Effectiveness Of Executive Function Performance Test (Efpt) In Stroke Population, Faryal Shaheen

Occupational Therapy Graduate Student Evidenced-Based Research Reviews

Stroke is the leading cause of long term disability in the U.S, causing 15,000 deaths each year (Hall et al, 2015). It has been estimated that 71% of clients are discharged with minimal to no services from acute/subacute after stroke because of deficits that go undetected (2015).

One of the most common deficits in stroke is loss in executive functioning (EF). Occupational therapists (OTs) have typically used a combination of tools to measure EF and most of these assessments did not involve observations in the everyday environment (Morrison et al, 2015). A new measure, known as the Executive Function Performance …

Effects Of Reducing Fatigue In Muscular Dystrophy Through Physical Activity, Callie Toaso, Alea Brown

Occupational Therapy Graduate Student Evidenced-Based Research Reviews

Myopathy is a functional impairment of muscle tissue; muscular dystrophy is a type of myopathy (Rakowicz & Lane, 2004). Muscular dystrophy (MD) consists of progressive muscle weakness and affects limbs and facial muscles. The most common types include Duchenne muscular dystrophy, Fascioscapulohumeral muscular dystrophy (FSHD), and Limb-girdle muscular dystrophy (Mercuri & Muntoni, 2013). One common complaint found with patients with muscular dystrophy is fatigue. It is noted that fatigue affects daily activities, but it is believed that physical activity can help reduce this. The purpose of this systematic review is to research how effective physical activity is at reducing fatigue …

Deer In Their Own Coats, Daniel Coleman

The Goose

Urban deer are requiring a renegotiation of settler-Six Nations relations in Hamilton, Ontario. In this article, Daniel Coleman attempts to get to know one doe group that share his neighbourhood in an effort to understand what their presence has to say about how Hamiltonians and members of the Hodinoso:ni Confederacy can honour the spirit of an eighteenth-century treaty in ways that enable us all to live with "the good mind" here at the Head of Lake Ontario in the twenty-first century.

Cells Too Stiff To Scavenge Leads To Lupus, An Autoimmune Disease, Jeff Hansen

Browse All News

No abstract provided.

Estrogen Receptor–Α In Medial Amygdala Neurons Regulates Body Weight, Pingwen Xu, Xuehong Cao, Yanlin He, Liangru Zhu, Yongjie Yang, Kenji Saito, Chunmei Wang, Xiaofeng Yan, Antentor Othrell Hinton Jr., Fang Zou, Hongfang Ding, Yan Xia, Chunling Yan, Gang Shu, San-Pin Wu, Bin Yang, Benjamin Feng, Deborah J. Clegg, Richard Demarchi, Sohaib A. Khan, Sophia Y. Tsai, Francesco J. Demayo, Qi Wu, Qingchun Tong, Yong Xu

Peer Reviewed Articles

Estrogen receptor–α (ERα) activity in the brain prevents obesity in both males and females. However, the ERα-expressing neural populations that regulate body weight remain to be fully elucidated. Here we showed that single-minded–1 (SIM1) neurons in the medial amygdala (MeA) express abundant levels of ERα. Specific deletion of the gene encoding ERα (Esr1) from SIM1 neurons, which are mostly within the MeA, caused hypoactivity and obesity in both male and female mice fed with regular chow, increased susceptibility to diet-induced obesity (DIO) in males but not in females, and blunted the body weight–lowering effects of a glucagon-like peptide-1–estrogen …

Fresolimumab Treatment Decreases Biomarkers And Improves Clinical Symptoms In Systemic Sclerosis Patients, Lisa M. Rice, Cristina M. Padilla, Sarah R. Mclaughlin, Allison Mathes, Jessica Ziemek, Salma Goummih, Sashidhar Nakerakanti, Michael York, Giuseppina Farina, Michael L. Whitfield, Robert F. Spiera, Romy B. Christmann, Jessica K. Gordon, Janice Weinberg, Robert Lafyatis

Dartmouth Scholarship

BACKGROUND. TGF-β has potent profibrotic activity in vitro and has long been implicated in systemic sclerosis (SSc), as expression of TGF-β–regulated genes is increased in the skin and lungs of patients with SSc. Therefore, inhibition of TGF-β may benefit these patients.

METHODS. Patients with early, diffuse cutaneous SSc were enrolled in an open-label trial of fresolimumab, a high-affinity neutralizing antibody that targets all 3 TGF-β isoforms. Seven patients received two 1 mg/kg doses of fresolimumab, and eight patients received one 5 mg/kg dose of fresolimumab. Serial mid-forearm skin biopsies, performed before and after treatment, were analyzed for expression …

A Cysteine Zipper Stabilizes A Pre-Fusion F Glycoprotein Vaccine For Respiratory Syncytial Virus, Guillaume B. E. Stewart-Jones, Paul V. Thomas, Man Chen, Aliaksandr Druz, Gordon M. Joyce, Wing-Pui Kong, Mallika Sastry, Conque Soto, Yongping Yang, Baoshan Zhang, Lei Chen, Gwo-Yu Chuang, Ivelin S. Georgiev, Jason S. Mclellan

Dartmouth Scholarship

Recombinant subunit vaccines should contain minimal non-pathogen motifs to reduce potential off-target reactivity. We recently developed a vaccine antigen against respiratory syncytial virus (RSV), which comprised the fusion (F) glycoprotein stabilized in its pre-fusion trimeric conformation by “DS-Cav1” mutations and by an appended C-terminal trimerization motif or “foldon” from T4-bacteriophage fibritin. Here we investigate the creation of a cyste- ine zipper to allow for the removal of the phage foldon, while maintaining the immunogenic- ity of the parent DS-Cav1+foldon antigen. Constructs without foldon yielded RSV F monomers, and enzymatic removal of the phage foldon from pre-fusion F trimers resulted in …

Gonad: Genome-Editing Via Oviductal Nucleic Acids Delivery System: A Novel Microinjection Independent Genome Engineering Method In Mice., Gou Takahashi, Channabasavaiah B. Gurumurthy, Kenta Wada, Hiromi Miura, Masahiro Sato, Masato Ohtsuka

Journal Articles: Genetics, Cell Biology & Anatomy

Microinjection is considered the gold standard technique for delivery of nucleic acids (NAs; transgenes or genome editing tools such as CRISPR/Cas9 systems) into embryos, for creating genetically modified organisms. It requires sophisticated equipment as well as well-trained and highly skilled personnel to perform the micro-injection technique. Here, we describe a novel and simple microinjection-independent technique, called Genome-editing via Oviductal Nucleic Acids Delivery (GONAD). Using GONAD, we show that NAs (e.g., eGFP mRNA or Cas9 mRNA/sgRNAs) can be effectively delivered to pre-implantation embryos within the intact mouse oviduct by a simple electroporation method, and result in the desired genetic modification in …

The Experiences Of Students With Autism Spectrum Disorders In College: A Heuristic Exploration, Fleur Wiorkowski

The Qualitative Report

This inquiry was conducted to describe the experience of individuals with autism spectrum disorders who have experienced the higher education system. All participants have been diagnosed with an autism spectrum disorder and experienced some aspect of the higher education system. Data was collected using primarily face to face interviews. The data was then analyzed using the heuristic methodology of Dr. Clark Moustakas (1990). Ultimately, many patterns and themes emerged from this analysis, culminating in a creative synthesis which sums up the experience. The themes showed the highs and lows of being a college student coupled with the experience of having …

“What’S Race Got To Do With It?” An Investigation Into The Differences Between Self-Reported Television Exposure And Self-Reported Attitudes Towards Interracial Dating, Kimberly Reeb

Theses

Television has the power to suggest what its viewers should think about and to impact individuals’ attitudes towards controversial topics. The present study examined the differences between individuals’ self-reported television exposure and self-reported interracial dating attitudes (IDA). Television viewing habits and demographics were also analyzed. A survey distributed via social media and email collected 216 responses. Cultivation theory suggests heavy viewers are likely to have more negative perceptions and attitudes. Analysis of differences for IDA between viewers was not statistically significant, but the IDA score was slightly more negative for heavy viewers. Overall, IDA scores were positive, however individuals’ attitude …

Dialogical Interspecies Ethics: Ataraxia, Desire And Hope In The Post-Human World Of Anne Carson's Pastoral, Thomas Bristow Dr

The Goose

This review essay implicitly revisits human and non-human power relations within a critical animal studies context that understands the affective conjunction between the manipulation of our worlds (action, partly through knowledge) and degrees of involvement with these others that live in our worlds (comportment via emotions). I take Louise Westling’s new study as the platform for an analysis of two book-length poems, The Autobiography of Red (1998) and red doc> (2013), which centre on the life of a shepherd, Geryon. Rather than revisit classical pastoral, these texts extract power-relations that classical myth and pastoral spatialise. In so doing, I argue, …