Digital Commons Network^™

Integrins Promote Cytokinesis Through The Rsk Signaling Axis, Shomita S. Mathew, Bethsaida Nieves, Sharon Sequeira, Savitha Sambandamoorthy, Kevin Pumiglia, Melinda Larsen, Susan E. Laflamme

Biological Sciences Faculty Scholarship

Cytokinesis is the final stage in cell division. Although integrins can regulate cytokinesis, the mechanisms involved are not fully understood. In this study, we demonstrate that integrin-regulated ERK (extracellular signal-related kinase) and RSK (p90 ribosomal S6 kinase) signaling promotes successful cytokinesis. Inhibiting the activation of ERK and RSK in CHO cells by a mutation in the integrin b1 cytoplasmic tail or with pharmacological inhibitors results in the accumulation of cells with midbodies and the formation of binucleated cells. Activation of ERK and RSK signaling by the expression of constitutively active RAF1 suppresses the mutant phenotype in a RSK-dependent manner. Constitutively …

Recent Development: In Re Victoria C.: Children Are Third Parties For Purposes Of Child Visitation; Siblings Must Make A Prima Facie Case Of Parental Unfitness Or Exceptional Circumstances Before Applying The Best Interest Of Child Standard, Allyson Bloom

University of Baltimore Law Forum

The Court of Appeals of Maryland held that a child is considered a third party for purposes of sibling visitation rights. In re Victoria C., 437 Md. 567, 591, 88 A.3d 749, 764(2014). The court further held that the sibling seeking visitation with a minor sibling must first make a prima facie case showing parental unfitness or exceptional circumstances before the court can apply the best interest of the child standard.

President's Report Card - April 2014, Clemson University

President's Reports to the Board of Trustees

No abstract provided.

President's Report Card - July 2014, Clemson University

President's Reports to the Board of Trustees

No abstract provided.

The Relationship Between Collective Action And Well-Being And Its Moderators: Pervasiveness Of Discrimination And Dimensions Of Action, Mindi D. Foster

Psychology Faculty Publications

Given the negative impact of perceiving gender discrimination on health (e.g., Pascoe & Smart Richman, 2009), there is a need to develop interventions to attenuate this effect; collective action may be one such intervention. Study 1 (N = 185) used an experimental paradigm to investigate whether undergraduate women in Waterloo, Ontario, Canada perceived pervasiveness of discrimination would interact with their collective action-taking to predict negative mood and well-being. Results showed that among those perceiving pervasive gender discrimination, informing friends/family and informing the media led to greater well-being than doing nothing, whereas among those perceiving gender discrimination as isolated, doing nothing …

Relationship Of Follicle Size And Concentrations Of Estradiol Among Cows Exhibiting Or Not Exhibiting Estrus During A Fixed-Time Ai Protocol, G. A. Perry, O. L. Swanson, E. L. Larimore, B. L. Perry, G. D. Djira, R. A. Cushman

Roman L. Hruska U.S. Meat Animal Research Center: Reports

Cows exhibiting estrus near fixed-time artificial insemination (AI) had greater pregnancy success than cows not showing estrus. The objective of this study was to determine the relationship between follicle size and peak estradiol concentration between cows that did or did not exhibit estrus during a fixed-time AI protocol. Ovulation was synchronized in beef cows by applying the CO-Synch protocol [GnRH (100 mg) on day-9, prostaglandin F2a (PGF_2α; 25 mg) on day-2, and a second injection of GnRH 48 h after PGF_2α (day 0)] to both suckled (experiment 1) and nonsuckled (experiment 2) cows. Follicle size (day 0) …

Environmental Issues In The Allocation And Management Of Western Interstate Rivers, Reed D. Benson

Faculty Scholarship

No abstract provided.

Development Of Loop-Mediated Isothermal Amplification Combined With Colorimetry Using Nanogold Probe For Chlamydia Trachomatis Detection, Ilada Choopara

Chulalongkorn University Theses and Dissertations (Chula ETD)

Chlamydia trachomatis จัดเป็นแบคทีเรียที่ก่อโรคติดต่อทางเพศสัมพันธ์ที่พบมากที่สุดทั่วโลกทั้งในเพศหญิงและชาย ถึงแม้ว่าโรคติดเชื้อนี้จะไม่ทําให้ผู้ติดเชื้อถึงตายและไม่แสดงอาการเริ่มต้นหรือแสดงเพียงเล็กน้อยนั้น การติดเชื้อนี้ในระยะยาวมีส่วนทําให้เป็นหมันหรือมีบุตรยาก และส่งเสริมการเกิดมะเร็งปากมดลูกจากไวรัสเอชพีวีได้ จึงเป็นที่น่าเสียดายที่การตรวจสุขภาพประจําปียังไม่มีการตรวจเชื้อนี้รวมอยู่ด้วย และยังไม่มีวิธีการตรวจเชื้อนี้ในคลินิกหรือสถานีอนามัยต่างๆ ที่การตรวจเชื้อนี้มีความสําคัญ ปัจจุบันวิธีมาตรฐานที่ใช้ตรวจคือการวิเคราะห์อาการโดยแพทย์ หรือการวิเคราะห์ลําดับเบสของยีน ompA (วิธีนี้ประกอบด้วยขั้นตอนการสกัดดีเอ็นเอด้วยชุดสกัดสําเร็จรูป การทําปฏิกิริยาลูกโซ่โพลีเมอเรส การรันเจลอิเล็กโตรโฟริซิส และการหาลําดับเบส) ดังนั้น งานวิจัยนี้จึงได้พัฒนาวิธีที่ไม่ซับซ้อนและประหยัดค่าใช้จ่ายขึ้นจากเทคนิคลูปเมดิเอเตดไอโซเทอร์มอลแอมพลิฟิเคชัน (LAMP) ร่วมกับการวัดสีโดยใช้โพรบติดอนุภาคทองระดับนาโน (AuNP) พบว่าวิธีที่พัฒนาขึ้นให้ผลความจําเพาะและความไวเทียบเท่าวิธีมาตรฐาน และใช้เวลาเพียง 24.5 นาที ขณะที่วิธีมาตรฐานใช้เวลามากกว่า 24 ชั่วโมง เมื่อรวมกับวิธีสกัดดีเอ็นเออย่างหยาบที่เหมาะสมกับชนิดของตัวอย่างพบว่าใช้เวลารวมทั้งสิ้นเพียง 29.5 นาที และมีค่าใช้จ่ายเพียง $3.6 ขณะที่วิธีมาตรฐานราคา $13.8 นอกจากนี้เมื่อทําการสุ่มตรวจในกลุ่มคนไข้และกลุ่มคนปกติ จํานวน 130 คน พบว่าวิธีที่พัฒนาขึ้นให้ผลตรงกับวิธีมาตรฐาน

Quality And Safety Of Minimally Invasive Surgery: Past, Present, And Future, Bernadette Mccrory, Chad A. Lagrange, M. S. Hallbeck

Department of Mechanical and Materials Engineering: Faculty Publications

Adverse events because of medical errors are a leading cause of death in the United States (US) exceeding the mortality rates of motor vehicle accidents, breast cancer, and AIDS. Improvements can and should be made to reduce the rates of preventable surgical errors because they account for nearly half of all adverse events within hospitals. Although minimally invasive surgery (MIS) has proven patient benefits such as reduced postoperative pain and hospital stay, its operative environment imposes substantial physical and cognitive strain on the surgeon increasing the risk of error. To mitigate errors and protect patients, a multidisciplinary approach is needed …

Measurement Of Hydrodynamic Force Generation By Swimming Dolphins Using Bubble Dpiv, Frank E. Fish, Paul Legac, Terrie M. Williams, Timothy Wei

Department of Mechanical and Materials Engineering: Faculty Publications

Attempts to measure the propulsive forces produced by swimming dolphins have been limited. Previous uses of computational hydrodynamic models and gliding experiments have provided estimates of thrust production by dolphins, but these were indirect tests that relied on various assumptions. The thrust produced by two actively swimming bottlenose dolphins (Tursiops truncatus) was directly measured using digital particle image velocimetry (DPIV). For dolphins swimming in a large outdoor pool, the DPIV method used illuminated microbubbles that were generated in a narrow sheet from a finely porous hose and a compressed air source. The movement of the bubbles was tracked …

Is Burnout A Depressive Disorder? A Reexamination With Special Focus On Atypical Depression, Renzo Bianchi, Irvin Sam Schonfeld, Eric Laurent

Publications and Research

Whether burnout and depression cover the same psychopathology remains to be elucidated. To date, subtypes of depression have been overlooked in research on the burnout–depression overlap. Our aim was to estimate the prevalence of depressive disorders in workers with burnout while examining the overlap of burnout with the atypical subtype of depression. The present study included 5,575 schoolteachers (mean age = 41 years; 78% female). Burnout was assessed with the Maslach Burnout Inventory. Depression was measured with the 9-item depression scale of the Patient Health Questionnaire (PHQ-9). Atypical features of depression were examined using a dedicated module, referenced to …

Natural And Lesion-Induced Decrease In Cell Proliferation In The Medial Nucleus Of The Trapezoid Body During Hearing Development, Aminat Saliu, Shana Adise, Sandy Xian, Kamila Kudelska, Adrián Rodríguez-Contreras

Publications and Research

The functional interactions between neurons and glial cells that are important for nervous system function are presumably established during development from the activity of progenitor cells. In this study we examined proliferation of progenitor cells in the medial nucleus of the trapezoid body (MNTB) located in the rat auditory brainstem. We performed DNA synthesis labeling experiments to demonstrate changes in cell proliferation activity during postnatal stages of development. An increase in cell proliferation correlated with MNTB growth and the presence of S100β-positive astrocytes among MNTB neurons. In additional experiments we analyzed the fate of newly born cells. At perinatal ages, …

Interactions Between African Americans And Police Officers: How Cultural Stereotypes Create A Wrongful Conviction Pipeline For African Americans, Cynthia J. Najdowski

Psychology Faculty Scholarship

Erroneous convictions are not as rare as one might expect and when they occur, the wrongfully accused are more often African American than White: Of those who were wrongfully convicted and later exonerated in the last quarter century, 47% were African American (The National Registry of Exonerations, 2013), even though only 13% of the U.S. population is (Rastogi, Johnson, Hoeffel, & Drewery, 2011). Yet Gould, Carrano, Leo, and Young’s (2013) recent analysis of miscarriages of justice indicated that race does not reliably differentiate between cases in which innocent defendants are wrongfully convicted as opposed to rightfully acquitted. They suggested that, …

Untangling Intra- And Interspecific Effects On Body Size Clines Reveals Divergent Processes Structuring Convergent Patterns In Anolis Lizards, Martha M. Muñoz, Johanna E. Wegener, Adam C. Algar

Biological Sciences Faculty Publications

Bergmann’s rule—the tendency for body size to increase in colder environments—remains controversial today, despite 150 years of research. Considerable debate has revolved around whether the rule applies within or among species. However, this debate has generally not considered that clade-level relationships are caused by both intra- and interspecific effects. In this article, we implement a novel approach that allows for the separation of intra- and interspecific components of trait-environment relationships. We apply this approach to body size clines in two Caribbean clades of Anolis lizards and discover that their similar body size gradients are constructed in very different ways. We …

Reflective Strength-Giving Dialogue Developed To Support Older Adults In Learning To Live With Long-Term Pain: A Method And A Study Design, Catharina Gillsjö, M. Berglund

College of Nursing Faculty Publications

Background: Long-term musculoskeletal pain is a major health problem that significantly impacts quality of life among older adults. Many lack professional guidance and must learn on their own to live with pain. This calls for a holistic method that addresses older adults’ needs in their situations. The developed method has its foundation in the didactic model: “The challenge – to take control of one’s life with long-term illness.

Aim: The aim was to describe the method, Reflective STRENGTH-Giving Dialogue, and present a study design where the method is learned and used by health careprovidersto support older adults in learning to …

Aboard Gso: A Newsletter For Alumni And Friends Of The University Of Rhode Island’S Graduate School Of Oceanography For Spring 2014, Uri Graduate School Of Oceanography

Graduate School of Oceanography Publications

The Spring 2014 (Volume 12, Issue 1) issue of Aboard GSO: A Newsletter for Alumni and Friends of the University of Rhode Island’s Graduate School of Oceanography, a periodical about GSO events, faculty research and essays by alumni.

Infantile Exposure To Lead And Late-Age Cognitive Decline: Relevance To Ad, Syed Waseem Bihaqi, Azadeh Bahmani, Gehad M. Subaiea, Nasser H. Zawia

Biomedical and Pharmaceutical Sciences Faculty Publications

Background: Early-life lead (Pb) exposure induces overexpression of the amyloid beta precursor protein and its amyloid beta product in older rats and primates. We exposed rodents to Pb during different life span periods and examined cognitive function in old age and its impact on biomarkers associated with Alzheimer's disease (AD).

Methods: Morris, Y, and the elevated plus mazes were used. Western blot, quantitative polymerase chain reaction (qPCR), and enzyme-linked immunosorbent assay were used to study the levels of AD biomarkers.

Results: Cognitive impairment was observed in mice exposed as infants but not as adults. Overexpression of AD-related genes (amyloid beta …

Multifaceted Nutritional Intervention In Hemodialysis Patients, Zulfitri Azuan Mat Daud

Wayne State University Dissertations

Mortality rate in patients undergoing chronic hemodialysis (HD) in the United States remain unacceptably high despite improvement in dialysis technology. Cardiovascular disease (CVD) account for more than 50% of the premature death in this population. Evidences indicate that the accelerated CVD are attributed to by malnutrition, inflammation, dyslipidemia and oxidative stress. Dietary intervention using nutrients with antioxidant, anti-inflammatory, and potential lipid altering properties to correct the aforementioned problems remain inconclusive. Furthermore, such nutritional intervention trial is often hampered by poor compliance related to medical and socioeconomic barriers. Therefore, a series of randomized, double-blind, placebo-controlled, parallel trials were undertaken to document …

Assembly And Function Of Macromolecular Complexes For Accurate Trna Aminoacylation In Helicobacter Pylori, Gayathri Niroshani Silva

Wayne State University Dissertations

ABSTRACT

ASSEMBLY AND FUNCTION OF MACROMOLECULAR COMPLEXES FOR ACCURATE TRNA AMINOACYLATION IN HELICOBACTER PYLORI

by

GAYATHRI SILVA

January 2014

Advisor: Dr. Tamara L. Hendrickson

Major: Chemistry (Biochemistry)

Degree: Doctor of Philosophy

Abstract

The aminoacylation of tRNA is a critical step in maintaining the accuracy of the genetic code. Many microorganisms are missing one or more aminoacyl tRNA synthetases (aaRSs) and rely on indirect pathways to produce certain aa–tRNAs. In Helicobacter pylori (H. pylori), the genes encoding both asparaginyl tRNA synthetase (AsnRS) and glutaminyl tRNA synthetase (GlnRS) are missing and the organism consequently relies on the indirect pathway for …

Empathy As A Moderator Of Adolescent Bullying Behavior And Moral Disengagement After Controlling For Social Desirability, Amy Zelidman

Wayne State University Dissertations

The purpose of this study was to assess the moderating influence empathy has on the associations between adolescent bullying behavior and moral disengagement after controlling for social desirability (e.g., response bias). 676 students in 7th and 8th grade from a suburban middle school in Southeast Michigan participated in this study in the fall of 2012.

Results showed male respondents were more likely than female respondents to (a) report engaging in all forms of traditional bullying behavior overall, including physical, verbal, and social bullying and (b) report higher rates of physical victimization and moral disengagement. Female respondents were more likely to …

The Energy Trilogy: An Integrated Sustainability Model To Bridge Wastewater Treatment Plant Energy And Emissions Gaps, A. Adhim A. Altalibi

Wayne State University Dissertations

ABSTRACT

An estimated 4% of national energy consumption is used for drinking water and wastewater services. Despite the awareness and optimization initiatives for energy conservation, energy consumption is on the rise owing to population and urbanization expansion and to commercial and industrial business advancement. The principal concern is since energy consumption grows, the higher will be the energy production demand, leading to an increase in CO2 footprints and the contribution to global warming potential.

This research is in the area of energy-water nexus, focusing on wastewater treatment plant (WWTP) energy trilogy - the group of three related entities, which includes …

Accumulation Of Subcortical Iron As A Modifier Of Volumetric And Cognitive Decline In Healthy Aging: Two Longitudinal Studies, Ana Marie Daugherty

Wayne State University Dissertations

Accumulation of non-heme iron in the brain has been theorized as a cellular mechanism underlying global neural and cognitive decline in normal aging and neurodegenerative disease. Relatively few studies of brain iron in normal aging exist and extant studies are almost exclusively cross-sectional. Here, I estimated iron content via T2* and measured volumes in several brain regions in two independent samples of healthy adults. The first sample (N = 89) was measured twice with a two-year delay; and the second sample (N = 32) was assessed four times over a span of 7 years. Latent models estimated change in iron …

Uracil Accumulation Response To Folate Depletion Is A Function Of Dna Repair Imbalance That May Be A Mechanism Of Aging, Hongzhi Ma

Wayne State University Dissertations

Folate is an essential nutrient for human. They carry and transfer one carbon units. They has important biological functions during many reactions, like DNA synthesis, RNA synthesis, DNA methylation, DNA repair, cell division and growth. Through biochemical techniques to determine the gene expression, enzyme activity, uracil level in DNA, and through microbiological assay to determine the folate level, elucidates mechanism of uracil accumulation response to folate depletion. The findings present folate deficiency status affects the uracil DNA glycosylase activity, and uracil initiated DNA base excising repair. DNA repair ability decide whether there is uracil accumulation under the folate depletion condition …

Development And Application Of Biomimetic Electrospun Nanofibers In Total Joint Replacement, Wei Song

Wayne State University Dissertations

Failure of osseointegration (direct anchorage of an implant by bone formation at the bone-implant surface) and implant infection (such as that caused by Staphylococcus aureus, S. aureus) are the two main causes of implant failure and loosening. There is a critical need for orthopedic implants that promote rapid osseointegration and prevent bacterial colonization, particularly when placed in bone compromised by disease or physiology of the patients. A better understanding of the key factors that influence cell fate decisions at the bone-implant interface is required. Our study is to develop a class of "bone-like" nanofibers (NFs) that promote osseointegration while preventing …

Understanding The Role Of Nutritional Stress In The Adult And Developing Zebra Finch, Jessica Bayley Thompson

Wayne State University Dissertations

Songbirds are particularly susceptible to stress during the sensitive period for song learning. Thus the developmental stress hypothesis (DSH) proposes that adult song reflects a male's early life environment during this period. Nutritional stress (NS) has been shown to cause deficits in song learning and adult song output that are salient to females. Female song birds consistently prefer control males over those raised under NS, yet the effects NS on females are still unclear. The hypothalamic-pituitary-adrenal axis (HPA) has been implicated in this process. However, evidence directly linking HPA functioning to the deficits due to NS have inconsistently shown elevations …

Development Of Active Packaging With Antimicrobial Activity, Xiuxiu Sun

Wayne State University Dissertations

Microbial safety of fresh fruits and vegetables is an increasing concern with any possible recall or outbreaks negatively affecting the entire industry. The interest in the development of active packaging has recently been steadily increasing due to significant concerns about environmental pollution caused by non-biodegradable packaging materials and consumer demand for high quality food products. Newly developed packaging materials and methods often have additional functional properties, such as antioxidant and antimicrobial properties.

In our study, the chitosan-gallic acid edible films and chitosan-essential oil coatings with antimicrobial activity, as well as active packaging with chlorine dioxide sachet were developed. The results …

Self-Construal Moderates Testosterone Reactivity To Competitive Outcomes, Keith Welker

Wayne State University Dissertations

Previous research shows that testosterone reactivity to competitive outcomes predicts aggressive behavior in men. However, some studies have failed to find these effects, and it has been suggested that individual differences moderate the relationships between competitive outcomes, testosterone fluctuations, and aggressive behavior. The current research examined whether one individual difference--self-construal--would moderate these effects. In Study 1, participants were assigned to win or lose a competitive video game and engaged in a reactive aggression task. Results indicated that increases in testosterone in response to winning and decreases in response to losing occurred in men with independent, not interdependent, self-construals. These changes …

Effects Of Altering The Peroxisomal Redox State In Models Of Degenerative Disease, Courtney Rose Giordano

Wayne State University Dissertations

Peroxisomes are important regulators of cellular redox balance and function as a signaling platform to regulate anti-aging metabolic and communication networks. In addition the organelle has emerged as a major player in maintaining cellular ROS at an optimal level. At such levels, these ROS are involved in initiation of signaling cascades and that produce an array of anti-aging and disease processes. However, as cells age over time, ROS amass within the peroxisome and elsewhere in the cell. This leads to an imbalance in oxidative homeostasis and results in compromised signaling networks. The goal of this dissertation was to treat disease …

Experimental And Theoretical Studies Of Binding Interactions In Divalent Transition Metal Cation-N-Donor Ligand Complexes: Structures, Sequential Bond Dissociation Energies, Mechanisms And Energetics Of Collision-Induced Dissociation, Holliness Nose

Wayne State University Dissertations

The thesis research described here involves a series of experiments that have been designed to probe the influence of the electronic structure of the metal cation, the nature and number of ligands, as well as the effects of chelation and steric interactions on the geometry and binding strength of transition metal cation-ligand complexes. The experimental studies make use of energy-resolved collision-induced dissociation (CID) techniques that are carried out in a custom-built guided ion beam tandem mass spectrometer (GIBMS) to probe the structures, energetics, and fragmentation behavior of the complexes of interest. Electronic structure theory calculations including several density functional theory …

An Analysis Of Self: The Development And Assessment Of A Measure Of Selfobject Needs, Eamonn Arble

Wayne State University Dissertations

The theory of self psychology as advanced by Heinz Kohut (1971, 1977, 1984) emphasized the importance of early developmental experiences, most centrally, the empathic attunement of caregivers as key nutrients for fostering the capacity for healthy self-regulation and interpersonal relations. Kohut elaborated three critical selfobject needs (i.e., idealizing, mirroring, and twinship) that were ideally satiated by these early experiences, deeming the fulfillment of these needs as essential for developing a healthy sense of self, capable of sustaining ambitions, self-soothing, and caring for others and oneself. In the case of psychopathology, these needs were theorized to have gone unmet, leaving the …