Digital Commons Network^™

Near-Infrared Imaging Of Adoptive Immune Cell Therapy In Breast Cancer Model Using Cell Membrane Labeling, Fatma M. Youniss, Gobalakrishnan Sundaresan, Laura J. Graham, Li Wang, Collin R. Berry, Gajanan K. Dewkar, Purnima Jose, Harry D. Bear, Jamal Zweit

Radiology Publications

The overall objective of this study is to non-invasively image and assess tumor targeting and retention of directly labeled T-lymphocytes following their adoptive transfer in mice. T-lymphocytes obtained from draining lymph nodes of 4T1 (murine breast cancer cell) sensitized BALB/C mice were activated in-vitro with Bryostatin/Ionomycin for 18 hours, and were grown in the presence of Interleukin-2 for 6 days. T-lymphocytes were then directly labeled with 1,1-dioctadecyltetramethyl indotricarbocyanine Iodide (DiR), a lipophilic near infrared fluorescent dye that labels the cell membrane. Assays for viability, proliferation, and function of labeled T-lymphocytes showed that they were unaffected by DiR labeling. The DiR …

Multifunctional Skin-Like Electronics For Quantitative, Clinical Monitoring Of Cutaneous Wound Healing, Yoshiaki Hattori, Leo Falgout, Woosik Lee, Sung-Young Jung, Emily Poon, Jung Woo Lee, Ilyoun Na, Amelia Geisler, Divya Sadhwani, Yihui Zhang, Yewang Su, Xiaoqi Wang, Zhuangjian Liu, Jing Xia, Huanyu Cheng, R. Chad Webb, Andrew P. Bonifas, Philip Won, Jae-Woong Jeong, Kyung-In Jang, Young Min Song, Beatrice Nardone, Michael Nodzenski, Jonathan A. Fan, Yonggang Huang, Dennis P. West, Amy S. Paller, Murad Alam, Woon-Hong Yeo, John. A. Rogers

Mechanical and Nuclear Engineering Publications

Non-invasive, biomedical devices have the potential to provide important, quantitative data for the assessment of skin diseases and wound healing. Traditional methods either rely on qualitative visual and tactile judgments of a professional and/or data obtained using instrumentation with forms that do not readily allow intimate integration with sensitive skin near a wound site. Here, an electronic sensor platform that can softly and reversibly laminate perilesionally at wounds to provide highly accurate, quantitative data of relevance to the management of surgical wound healing is reported. Clinical studies on patients using thermal sensors and actuators in fractal layouts provide precise time-dependent …

Art In The Age Of Networks - Networks As A Way Of Thinking, Rabeya Jalil

Higher Education Resources

The theme-based and material-based units (with lessons and lesson sequences) propose a curriculum for one academic semester in an undergraduate visual arts school (for sophomore, 2nd year, or junior, 3rd year students). However the lessons could be modified and tailored to any age group developmentally. This curricular framework aims to foster collaboration (within individuals, materials and disciplines), explore networked pedagogy and networks in pedagogy as a collaborating force through and with the visual arts and explore the materiality of the code and the digital media. The course also engages with new media theory and literature, investigates the materiality of the …

Rock Inhibitor Is Not Required For Embryoid Body Formation From Singularized Human Embryonic Stem Cells, Giuseppe Pettinato, Wendy S. Vanden Berg-Foels, Ning Zhang, Xuejun Wen

Chemical and Life Science Engineering Publications

We report a technology to form human embryoid bodies (hEBs) from singularized human embryonic stem cells (hESCs) without the use of the p160 rho-associated coiled-coil kinase inhibitor (ROCKi) or centrifugation (spin). hEB formation was tested under four conditions: +ROCKi/+spin, +ROCKi/-spin, -ROCKi/+spin, and -ROCKi/-spin. Cell suspensions of BG01V/hOG and H9 hESC lines were pipetted into non-adherent hydrogel substrates containing defined microwell arrays. hEBs of consistent size and spherical geometry can be formed in each of the four conditions, including the -ROCKi/-spin condition. The hEBs formed under the -ROCKi/-spin condition differentiated to develop the three embryonic germ layers and tissues derived from …

Fatigue Resistibility And Stimulus Strength Using Intraspinal Microstimulation Vs. Intramuscular Stimulation In A Rat Model: Case Study, John Dube

Honors Theses, 1963-2015

Spinal Cord Injury is a devastating injury that has drastic effects on an individual’s daily living activities. The current devices and methods of direct muscle, peripheral nerve cuff, and epidural stimulation are bulky, easily fatigable, require large stimulation parameters, and/ or difficult to use. Intraspinal Micro stimulation (ISMS) suggests a more fatigue resistant method at lower stimulation parameters that will help restore locomotion, bladder control, and sexual function in an individual. The research conducted herein shows ISMS evokes a linear positive relationship between stimulus strength and muscle contraction force that is comparable to clinically used methods of Intramuscular stimulation. Research …

Preliminary Study Of Kleptoplasty In Foraminifera Of South Carolina, Shawnee Lechliter

Bridges: A Journal of Student Research

Recent studies of living foraminifera, microscopic aquatic protists, indicate that some species have the ability to steal photosynthetic plastids from other microorganism and keep them viable through a process called kleptoplasty. Studying the symbiotic relationships within these diverse protists gives insight not only into evolutionary history, but also their importance to the ecosystem. We determined the presence of these kleptoplastic species and identified presence and origin of sequestered plastids based on morphological identification and molecular data from samples collected at Waties Island, South Carolina. We identified two kleptoplastic genera (Elphidium and Haynesina) and two non-kleptoplastic genera (Ammonia and Quinqueloculina) present …

2014 Celebration Of Inquiry Program, Coastal Carolina University

Celebration of Inquiry

11th Celebration of Inquiry, March 31-April 3, 2014. Theme: The Human Spirit: Overcoming Adversity to Accomplish Greatness. 6th Annual Undergraduate Research Competition abstracts and schedule are included in the program.

A Selected Surveyo F Ocean Acidifcation's Effect On Coccolithophore And Other Marine Ecosystems, Taylor Zaw, Joel Wood, Anthony Gonzalez

Featured Research

With it's rapid rate of generation, the coccolithophore was expected to adapt to the altered pH levels relatively quickly. By analyzing several scientific studies concerning this algae's interaction with various water qualities, the species' response is clarified. These findings are compared to rudimentary data concerning pH levels taken along the coast of Malibu.

Leaf Foliar Absorption In Pentagramma Triangularis And Polypodium Californicum, Justin Choe, Carlos Larios, William Prorok, Emily Rose Pierce

Featured Research

In Riparian and moist zones of the Santa Monica Mountains of Southern California, ferns flourish. However, the Mediterranean climate they live in is subject to drought, and the area is currently facing the worst drought it has ever seen. With little moisture in the ground and streams drying up, these ferns are running out of sources of water. However, being on the coast, the area is subject to fog nightly. This research was done to see if ferns can do leaf foliar absorption, getting their water source from the air rather than the ground. We hypothesized that ferns do leaf …

Relating Leaf Temperature On Malosma Laurina To Leaf Proximity From Soil, Brooks O'Hea, Kevin Morgan

Featured Research

The Santa Monica Mountains are home to countless vegetation and plant species, many of which have been forced to adapt to southern California’s stressful environment. With recent droughts and a steady change in climate, an increase in leaf temperature within some species in the Santa Monica Mountains has been observed. One prominent plant found within the mountain range is the malosma laurina, or laurel sumac, the only species of the malosma genus. Malosma laurina is a perfect example of a specie adapting to its stressful environment. Constant brush and shrub fires within the chaparral area where it commonly grows have …

Comparison Of Photosynthetic Ability In Single And Double Palisade Parenchyma Leaves In Southern California, Vincent Quach, Ryan Sauer, Haley Smith, Christian Wright

Featured Research

Melaleuca quinquenervia is a myrtle (family Myrtaceae) with a propensity for invasiveness. The leaves feature an isobilateral orientation: vertically aligned with a layer of palisade parenchyma on both the adaxial and abaxial sides. Quercus agrifolia (Fagaceae) is a native evergreen with one layer of palisade parenchyma. Due to this structural difference, it was hypothesized that Melaleuca would have a higher rate of photosynthesis (Pn) and less reflectance (NDVI) of green light than Quercus. These two variables were compared using an unpaired t-test, yielding p-values of 0.1366 for NDVI and 0.04428 for photosynthetic rate. No significant difference was found between the …

Measuring Water Potential Among Crassula Ovata And Heteromeles Arbutifolia, Monét Mcnally, Stephanie Yakupitiyage, Jamille Lockhart

Featured Research

Chaparral shrubs are known for their abilities to withstand dry environments. Both the California native C3 plant, Heteromeles arbutifolia, also known as Hollywood, and the CAM plant, Crassula ovata, also know as a Jade plant, have the ability to thrive under a Mediterranean-like climate region. However, during severe cases of drought one plant may better adapted than the other. The purpose of this experiment is to determine whether Heteromeles arbutifolia or Crassula ovata is better able to withstand drought by measuring the dehydration rate. We calculated the leaf-area to mass ratio and the change in water potential of each species …

Relationship Between Canopy Location And Tensile Strength Of Leave Of Heteromeles Arbutifolia In Malibu, Ca, Elena Witham, Melinda Marchiano, Danielle Reynolds

Featured Research

Our hypothesis is that canopy location has an effect on the characteristic tensile strength of leaves, with the leaves on the top of the canopy having greater strength than the leaves on the bottom. We tested our experiment by sampling leaves from the top and bottom of three H. arbutifolia specimens, obtaining raw measurements of size, and testing the tensile strength of each leaf using the Instron. We performed t-tests on the data to determine if the average length, width, thickness, and tensile strength were statistically different depending on if our p values were < 0.05 when top and bottom leaves of the same tree were compared. We chose a chapparral stand removed from heavy human traffic and influence, eliminating as many extraneous variables as possible by taking leaves from the same tree of the same age from the same position on the branch and chose three trial specimen of similar height in the same area to reduce the possibility of varying sunlight exposure or water supply from affecting leaf morphology in a way that canopy position would not. The results show that blade length between top and bottom is statistically significantly different, while blade thickness and lateral strength at midsection were not statistically different. From our analysis of the data, it is clear that leaves on the bottom of the plant were longer and broader than leaves on the top, but we determined that canopy location does not have a major effect on tensile strength or thickness, supporting a null hypothesis.

Relationship Between Canopy Location And Tensile Strength Of Leaves Of Heteromeles Arbutifolia In Malibu, Ca, Elena Witham, Melinda Marchiano, Danielle Reynolds

Featured Research

Our hypothesis is that canopy location has an effect on the characteristic tensile strength of leaves, with the leaves on the top of the canopy having greater strength than the leaves on the bottom. We tested our experiment by sampling leaves from the top and bottom of three H. arbutifolia specimens, obtaining raw measurements of size, and testing the tensile strength of each leaf using the Instron. We performed t-tests on the data to determine if the average length, width, thickness, and tensile strength were statistically different depending on if our p values were < 0.05 when top and bottom leaves of the same tree were compared. We chose a chapparral stand removed from heavy human traffic and influence, eliminating as many extraneous variables as possible by taking leaves from the same tree of the same age from the same position on the branch and chose three trial specimen of similar height in the same area to reduce the possibility of varying sunlight exposure or water supply from affecting leaf morphology in a way that canopy position would not. The results show that blade length between top and bottom is statistically significantly different, while blade thickness and lateral strength at midsection were not statistically different. From our analysis of the data, it is clear that leaves on the bottom of the plant were longer and broader than leaves on the top, but we determined that canopy location does not have a major effect on tensile strength or thickness, supporting a null hypothesis.

Correlation Between Freezing Sites And Xylem Vessel Diameter For Three Chaparral Species Of The Santa Monica Mountains, Kaitlyn E. Sauer, Theodora V. Ordog, Nicole A.P.M.K.O.M. Nakamatsu

Featured Research

Coastal exposures of the Santa Monica Mountains rarely experience freezing temperatures

(0 °C) because of the ameliorating effects of the Pacific Ocean and seawater’s specific heat capacity. In contrast, inland sites of the Santa Monica Mountains frequently experience winter temperatures below -10 °C. This temperature gradient, from coast to inland, may be a major determinate of species distribution patterns. To investigate possible mechanisms by which freezing impacts chaparral distribution patterns, we examined xylem vessel diameter and vessel length of three chaparral species growing at inland freezing sites versus coastal nonfreezing sites (Malosma laurina, Umbellularia californica, and Ceanothus megacarpus). It has …

Measuring Photosynthesis To Evaluate Photoprotection By Anthocyanins In Malosma Laurina, Jorge Bojorkez-Calderon, Hannah Imson

Featured Research

The purpose of this investigation was to observe the differences between the photosynthetic rates and photo-protection of young, red, juvenile leaves of Malosma laurina, and compare it to young, green leaves. To accomplish this, the open-system of Li-6400 XT was brought out into the field to a shrub of Malosma laurina that was flourishing and had both red and green leaves present. Then, data of fluorescence, photosynthetic rate, and conductance was taken from both red leaves and green leaves, and the photosynthetic rates were compared. Through this investigation, we were able to quantify that in young, red leaves, which had …

The Effects Of Higher And Lower Co2 Levels In The Atmosphere On The Photosynthetic Rates And Stomatal Conductance Of Malosma Laurina In The Santa Monica Mountains, Victoria A. Thorsen, Maverick E. Turner, Colby P. Harriman

Featured Research

We hypothesize that higher CO2 levels will lead to a change in photosynthetic performance in the Malosma laurina population of the Santa Monica Mountains. We used one Malosma laurina that is across the street from university’s theme tower. We chose this specific plant because it was in the sun and it was in a well irrigated area. We selected a plant that is in non-shaded areas at 12:00 pm and then dark adapt them for 20 minutes. We selected an adult plant that was between four and six feet tall. We took the measurements from the upper leaves of the …

Relationship Between Dehydration Tolerance Of California Ferns And The Mechanical Strength Of Their Stipes, Helen I. Holmlund, Jarmila Pitterman, Stephen D. Davis

Featured Research

In vascular plants, water moves upwards through xylem vessels/tracheids due to negative pressures created by the evaporative pull of water. Under severe dehydrated, extreme negative pressures are known to cause xylem cavitation and embolism. In seed-bearing plants, the mechanical support of stem xylem has been shown to be a good predictor of cavitation resistance, presumably because sclerenchyma fibers buttress against micro-fracture or collapse of conduit walls. In spore-bearing plants, such as ferns, current anatomical theory indicates that mechanical support lies external to underlying xylem, in the outer hypodermal region, leaving the central xylem in ferns without any fiber support. In …

Vegfr-2 And Vegfr-3 Expression As A Function Of Jagged-1 Over-Expression In Huvec Cells, Caleb Stubbs

Featured Research

Angiogenesis is the sprouting of new blood vessels from pre-existing vessels initiated from vascular endothelial cells in response to exogenous chemical signals. Principle signals are vascular endothelial growth factors (VEGF) that direct cell growth and differentiation by binding to endothelial cell surface VEGF receptors (VEGFR). In Human Umbilical Vascular Endothelial Cells (HUVEC) initial sprouting cells or “tip cells” send signals to neighboring cells “stalk cells” responsible for sprout elongation. Multiple VEGF signals and receptors are known, but the main angiogenic signals are from VEGF-A through VEGFR-2 and VEGFR-3. Regulation of VEGF receptor expression can be influenced by the Notch signaling …

Effect Of Shear Stress Direction On Endothelial Function And Enos Phosphorylation In Soleus Feed Arteries, Blanca B. Perez, Jay Brewster, Jeffrey Jasperse

Featured Research

Blood flow feeding tissues and organs is closely regulated in order to meet metabolic and functional needs. Control of blood flow is accomplished by regulating the diameter of the arteries and arterioles feeding different organs. Several neural, hormonal, chemical and mechanical mechanisms contribute to the constriction and dilation of arteries. Shear stress, the frictional force created by streaming blood on the endothelial layer of arteries, is one of these mechanical mechanisms (1). Shear stress causes both acute and long term effects on endothelial cells (1,2,5).

Blood in arteries typically flows away from the heart towards organs (causing antegrade shear stress) …

Differential Survival Of Chaparral Shrub Species In Response To Severe Drought, Hannah Dario

Featured Research

In this experiment, we examined how the plants in the Santa Monica Mountains are responding to the driest period in recorded history for California. Our hypothesis was that plant species with the deepest roots would have highest survivorship. We thought this because deep-rooted shrubs will have greater access to soil moisture. This hypothesis was tested by collecting data on plant survival, in a mixed chaparral stand, containing eleven species, growing in the Green Valley Preserve off of Stunt road, in the Santa Monica Mountains. The chaparral stand was 21 years old, having last burned in 1993 (Old Topanga Canyon Fire). …

Effects Of Uv-Radiation On Feeding Behavior In Dendrobates Tinctorius, Taylor S. Stucky, Arthur Garnica

Featured Research

UV-B levels have recently been increasing with the depletion of the ozone layer. Recent data have shown that amphibians are especially susceptible to UV-B, with poison dart frogs being one focus in these studies. The purpose of this study was to determine if UV-B radiation affects the foraging behavior of poison dart frogs. This study was conducted with a captive population of juvenile Dendrobates tinctorius at Pepperdine University in Malibu, California. Frogs underwent one of three treatments: UV only, Food only, and UV and Food. UV only trials were conducted using two UV lights emitting approximately 1.2 uW/cm2 of UV …

Enzymatic Activity In The Chorion For Hatching In The California Grunion, Emily R. Pierce, Vince V. Quach, Karen L. Martin

Featured Research

Leuresthes tenuis is a small, silverside fish that spawns on the beaches during some of the highest tides of the summer months. Of the many unique traits to the species including that the eggs develop fully out of water to the point of hatching competence but will not hatch until presented with an environmental cue, which causes them to hatch in less than a minute. The purpose of this study is to better understand the role of enzymes called chorionases, which act to break down the chorion (egg membrane). I hypothesize that the chorion begins to weaken in this species …

The Effects Of Carbon Black On Cell Viability, Abigail Calixto, Jay Brewster

Featured Research

Carbon black (CB) is a type of nanoparticle that is found in air pollution and is a known environmental toxin. The purpose of this work is to evaluate whether CB exposure activates cell death via apoptosis in cultured cell lines, supporting future work focused upon assessing the signaling pathways that might be induced by this exposure. Using adenocarcinomic human alveolar basal epithelial (A549) and baby hamster kidney (BHK-21) cells, we hypothesized that carbon black exposure causes cell death and potentially stress signaling via the endoplasmic reticulum (ER). The cells were exposed to CB and data collected for varied doses and …

The Mechanical Strength Of Malosma Laurina Leaves Corresponds With Survivability During Extreme Drought, Alexander Booth, Alexander Corwin, Matthew Chung

Featured Research

With the recent extreme drought that Southern California is facing, many plants’ survivability adaptations have proven insufficient. The Malosma laurina plant is known for it’s incredible ability to survive through droughts and wildfires with it’s adaptations of resprouts regeneration and it’s roots that can reach a depth of 40 feet to gather deep ground waters. We theorized that the mechanical strength of the leaves directly relates to its survivability during droughts. To explore this theory we took focused our theory specifically on M. laurina resprouts. With two groups, one fully alive and one dying, we took six samples from six …

Ethnobotanical Promotion Of Fibroblast Growth Using Yerba Santa Extract, Carlie F. Dobbins, Paige A. Sutherland, Emily A. Woods

Featured Research

Ethnobotany is a promising way of discovering new drugs, drawing on the knowledge of generations of traditional healers. The healing qualities of Yerba Santa (Eriodictyon crassifolium) have been recognized for hundreds of years by Chumash Indians and Spanish missionaries, so much so that they came to call it “holy herb”. We decided to determine the efficacy of Yerba Santa by extracting potentially bioactive molecules in methanol and then using this extract to treat scales from goldfish (Carassius auratus) to test the hypothesis that the Yerba Santa extract would alter the growth of fibroblasts. Compared to scales treated with only modified …

Leaf Mechanical Strength Corresponds To Tissue Water Relations In Twelve Species Of California Ferns, Breahna M. Gillespie, Stephen D. Davis, Jarmila Pitterman

Featured Research

The dominant vegetation types in southern California’s coastal foothills are chaparral and costal sage scrub. Chaparral shrubs have mechanically strong evergreen leaves whereas coastal sage scrubs bear mechanical weak, facultative deciduous leaves. What about the ferns that live in the understory of these vegetation types, especially considering their adaptations to a summer dry, Mediterranean-type climate? We tested the hypothesis that some fern leaves are stronger than others and mechanically strong leaves are associated with greater dehydration tolerance. Twelve fern species were examined. Tissue water relations were assessed via pressure volume curves using Scholander-Hammel pressure chambers. We estimated osmotic potential at …

Ethnobotanical Promotion Of Fibroblast Growth Using Yerba Santa Extract, Carletta F. Dobbins, Paige A. Sutherland, Emily A. Woods

Featured Research

Ethnobotany is a promising method for discovering new drugs, drawing on the knowledge of generations of traditional healers. For hundreds of years, the Chumash people have lived in the coastal regions of California, becoming experts on the many uses of its natural resources. One such resource is the Yerba Santa plant (Eriodictyon crassifolium), which was used by the Chumash to treat a myriad of conditions including coughs, chest pain, and fever. It was also used as a poultice on wounds and cuts, suggesting that the plant has a stimulating effect on the growth of skin cells. Because of these qualities, …

An Examination Of Federal Sentencing Guidelines’ Treatment Of Mdma (“Ecstacy”), Alyssa Hennig

Belmont Law Review

This Note analyzes the MDMA drug equivalency ratio under the current Guidelines and argues that the ratio is based on incomplete and inaccurate information. Part I of this Note provides an overview of the United States Sentencing Commission (“the Commission”) and the United States Sentencing Guidelines (“the Guidelines”). Part II discusses the rise of MDMA and the legislative response. It also summarizes the Commission’s findings regarding the social and physiological harms of MDMA that formed the basis for the Commission’s decision about the appropriate MDMA drug equivalency ratio. Part III examines the errors in the Commission’s empirical analysis of the …

Whose Rights Should Prevail? Toward A Child-Centric Approach To Revocation Of Birthparent Consent In Domestic Infant Abortion, David L. Thibodeaux

Belmont Law Review

Though overhaul of adoption laws across the United States has been nearly universal, there is still no uniformity among the states in approach to voluntary relinquishment of parental rights: the very issue at the heart of the controversial cases that sparked reform. This Note attempts to track the development of domestic adoption laws as they affect birthparent consent in infant adoptions, the competing policies driving these developments, and the way states have attempted to reconcile that friction. Part I of this Note provides an underpinning of adoption terminology by outlining the actors involved, the basic elements required for infant adoption …