Digital Commons Network^™

Living Microbe Repository, Best Practices, Regulatory Compliance, Reference Material Collection, Shaolei Zang, Na Liu, Hongchun Wang, David N. Wald, Na Shao, Jingru Zhang, Daoxin Ma, Chunyan Ji, William Tse

Faculty & Staff Scholarship

Background

We previously demonstrated that 6-benzylthioinosine (6-BT) could induce the differentiation of a subset of acute myeloid leukemia (AML) cell lines and primary AML cells regardless of their cytogenetics. In this study we investigated whether Wnt signaling pathways played roles in 6-BT-induced differentiation of AML cells.

Methods

We induced differentiation of HL-60 leukemic cells and primary AML cells in vitro using 6-BT. Real-time PCR (qPCR), western blot, and luciferase assays were used to examine the molecules’ expression and biological activity in canonical and noncanonical Wnt signaling pathways. AML cell differentiation was measured by the Nitroblue tetrozolium (NBT) reduction assay.

Results …

Rationing Environmental Law In A Time Of Climate Change, Rachael E. Salcido

Loyola University Chicago Law Journal

Addressing climate change demands a fundamental change in United States energy policy and a major infrastructure for a renewable energy future. Yet the boom in natural gas development and expanding demand for energy in developing nations argue poorly for reductions in fossil fuel use. This Article documents how the federal government has resorted to some measure of environmental exceptionalism by rationing environmental law to expedite renewable energy development in the context of the seismic shifts in U.S. energy policy. Despite the many arguments in opposition to rationing environmental law, this Article concludes that the realities of climate change and the …

A Thoreauvian Account Of Prudential Value, Christopher Morgan-Knapp

Philosophy Faculty Scholarship

This article develops and defends an account of prudential value and disvalue that is constructed out of ideas found in Thoreau’s Walden. The core claim is that prudential value consists in responding appropriately to those things that make the world better, and that prudential disvalue consists in encountering those things that make it worse. The core argument is that responding appropriately to objective value and avoiding objective disvalue are our aims in so far as we are evaluative creatures, and that our evaluative nature is fundamental in the context of inquiring into our own good. I conclude by discussing respects …

Amides Are Excellent Mimics Of Phosphate Internucleoside Linkages And Are Well Tolerated In Short Interfering Rnas, Daniel Mutisya, Chelliah Selvam, Benjamin Lunstad, Pradeep S. Pallan, Amanda Haas, Devin Leake, Martin Egli, Eriks Rozners

Chemistry Faculty Scholarship

RNA interference (RNAi) has become an important tool in functional genomics and has an intriguing therapeutic potential. However, the current design of short interfering RNAs (siRNAs) is not optimal for in vivo applications. Non-ionic phosphate backbone modifications may have the potential to improve the properties of siRNAs, but are little explored in RNAi technologies. Using X-ray crystallography and RNAi activity assays, the present study demonstrates that 3'-CH2-CO-NH-5' amides are excellent replacements for phosphodiester internucleoside linkages in RNA. The crystal structure shows that amide-modified RNA forms a typical A-form duplex. The amide carbonyl group points into the major groove and assumes …

Bacteria Present In Carotid Arterial Plaques Are Found As Biofilm Deposits Which May Contribute To Enhanced Risk Of Plaque Rupture, Bernard B. Lanter, Karin Sauer, David G. Davies

Biological Sciences Faculty Scholarship

Atherosclerosis, a disease condition resulting from the buildup of fatty plaque deposits within arterial walls, is the major underlying cause of ischemia (restriction of the blood), leading to obstruction of peripheral arteries, congestive heart failure, heart attack, and stroke in humans. Emerging research indicates that factors including inflammation and infection may play a key role in the progression of atherosclerosis. In the current work, atherosclerotic carotid artery explants from 15 patients were all shown to test positive for the presence of eubacterial 16S rRNA genes. Density gradient gel electrophoresis of 5 of these samples revealed that each contained 10 or …

Genome Sequence Of Microvirga Lupini Strain Lut6(T), A Novel Lupinus Alphaproteobacterial Microsymbiont From Texas, Wayne Reeve, Matthew Parker, Rui Tian, Lynne Goodwin, Hazuki Teshima, Roxanne Tapia, Cliff Han, James Han, Konstantinos Liolios, Marcel Huntemann, Amrita Pati, Tanja Woyke, Konstantinos Mavromatis, Victor Markowitz, Natalia Ivanova, Nikos Kyrpides

Biological Sciences Faculty Scholarship

Microvirga lupini LUT6(T) is an aerobic, non-motile, Gram-negative, non-spore-forming rod that can exist as a soil saprophyte or as a legume microsymbiont of Lupinus texensis. LUT6(T) was isolated in 2006 from a nodule recovered from the roots of the annual L. texensis growing in Travis Co., Texas. LUT6(T) forms a highly specific nitrogen-fixing symbiosis with endemic L. texensis and no other Lupinus species can form an effective nitrogen-fixing symbiosis with this isolate. Here we describe the features of M. lupini LUT6(T), together with genome sequence information and its annotation. The 9,633,614 bp improved high quality draft genome is arranged into …

Evaluation Of A Single Nucleotide Polymorphism Baseline For Genetic Stock Identification Of Chinook Salmon (Oncorhynchus Tshawytscha) In The California Current Large Marine Ecosystem, Anthony J. Clemento, Eric D. Crandall, John Carlos Garza, Eric C. Anderson

School of Natural Sciences Faculty Publications and Presentations

Chinook Salmon (Oncorhynchus tshawytscha) is an economically and ecologically important species, and populations from the west coast of North America are a major component of fisheries in the North Pacific Ocean. The anadromous life history strategy of this species generates populations (or stocks) that typically are differentiated from neighboring populations. In many cases, it is desirable to discern the stock of origin of an individual fish or the stock composition of a mixed sample to monitor the stock-specific effects of anthropogenic impacts and alter management strategies accordingly. Genetic stock identification (GSI) provides such discrimination, and we describe here …

Evolving Coral Reef Conservation With Genetic Information, Maria Beger, Kimberly A. Selkoe, Eric Treml, Paul H. Barber, Sophie Von Der Heyden, Eric D. Crandall, Robert J. Toonen, Cynthia Riginos

School of Natural Sciences Faculty Publications and Presentations

Targeted conservation and management programs are crucial for mitigating anthropogenic threats to declining biodiversity. Although evolutionary processes underpin extant patterns of biodiversity, it is uncommon for resource managers to explicitly consider genetic data in conservation prioritization. Genetic information is inherently relevant to management because it describes genetic diversity, population connectedness, and evolutionary history; thereby typifying their behavioral traits, physiological climate tolerance, evolutionary potential, and dispersal ability. Incorporating genetic information into spatial conservation prioritization starts with reconciling the terminology and techniques used in genetics and conservation science. Genetic data vary widely in analyses and their interpretations can be challenging even for …

Phylogeography Unplugged: Comparative Surveys In The Genomic Era, Brian W. Bowen, Kartik Shanker, Nina Yasuda, Maria Celia (Machel) D. Malay, Sophie Von Der Heyden, Gustav Paulay, Luiz A. Rocha, Kimberly A. Selkoe, Paul H. Barber, Suzanne T. Williams, Harilaos A. Lessios, Eric D. Crandall, Giacomo Bernardi, Christopher P. Meyer, Kent E. Carpenter, Robert J. Toonen

School of Natural Sciences Faculty Publications and Presentations

In March 2012, the authors met at the National Evolutionary Synthesis Center (NESCent) in Durham, North Carolina, USA, to discuss approaches and cooperative ventures in Indo-Pacific phylogeography. The group emerged with a series of findings: (1) Marine population structure is complex, but single locus mtDNA studies continue to provide powerful first assessment of phylogeographic patterns. (2) These patterns gain greater significance/power when resolved in a diversity of taxa. New analytical tools are emerging to address these analyses with multi-taxon approaches. (3) Genome-wide analyses are warranted if selection is indicated by surveys of standard markers. Such indicators can include discordance between …

Gust Mitigation Of Micro Air Vehicles Using Passive Articulated Wings, Adetunji Oduyela, Nathan Slegers

Faculty Publications - Biomedical, Mechanical, and Civil Engineering

Birds and insects naturally use passive flexing of their wings to augment their stability in uncertain aerodynamic environments. In a similar manner, micro air vehicle designers have been investigating using wing articulation to take advantage of this phenomenon. The result is a class of articulated micro air vehicles where artificial passive joints are designed into the lifting surfaces. In order to analyze how passive articulation affects performance of micro air vehicles in gusty environments, an efficient 8 degree-of-freedom model is developed. Experimental validation of the proposed mathematical model was accomplished using flight test data of an articulated micro air vehicle …

Identity-Related Dysfunction: Integrating Clinical And Developmental Perspectives, Erin A. Kaufman, Marilyn J. Montgomery, Sheila E. Crowell

Faculty Publications - Graduate School of Counseling

Recent changes to the American Psychiatric Association’s Diagnostic and Statistical Manual of Mental Disorders highlight the importance of identity dysfunction within several psychiatric diagnoses. Despite a long-standing tradition of identity research and theory in the developmental literature, there is limited work establishing intersections between clinical and developmental conceptualizations of identity problems. The relative lack of integration between decades of clinical and developmental work is unfortunate, and likely limits progress in both areas. In this commentary, the authors argue for greater interdisciplinary collaboration and highlight contributions from developmental and clinical theories, which, if integrated, could enhance identity scholarship. The developmental psychopathology …

There's Still Time To Get Your Feet Wet!, Valerie Cournoyer

Wrack Lines

Marine and aquatic field experiences provide a rich context for inquiry-based teaching and learning designed around standards.

The Health Sciences And Technology Academy: An Educational Pipeline To Address Health Care Disparities In West Virginia, Sherron Mckendall, Kassan Kasten, Sara Hanks, Ann Chester

Faculty & Staff Scholarship

Health and educational disparities are national issues in the United States. Research has shown that health care professionals from underserved backgrounds are more likely than others to work in underserved areas. The Association of American Medical Colleges’ Project 3000 by 2000, to increase the number of underrepresented minorities in medical schools, spurred the West Virginia School of Medicine to start the Health Sciences and Technology Academy (HSTA) in 1994 with the goal of supporting interested underrepresented high school students in pursuing college and health professions careers. The program was based on three beliefs: (1) if underrepresented high school students have …

Involvement Of Sigma-1 Receptors In The Antidepressant-Like Effects Of Dextromethorphan, Linda Nguyen, Matthew J. Robson, Jason R. Healy, Anna L. Scandinaro, Rae R. Matsumoto

Faculty & Staff Scholarship

Dextromethorphan is an antitussive with a high margin of safety that has been hypothesized to display rapid-acting antidepressant activity based on pharmacodynamic similarities to the N-methyl-D-aspartate (NMDA) receptor antagonist ketamine. In addition to binding to NMDA receptors, dextromethorphan binds to sigma-1 (σ1) receptors, which are believed to be protein targets for a potential new class of antidepressant medications. The purpose of this study was to determine whether dextromethorphan elicits antidepressant-like effects and the involvement of σ1 receptors in mediating its antidepressant-like actions. The antidepressant-like effects of dextromethorphan were assessed in male, Swiss Webster mice using the forced swim test. Next, …

Gender Recognition From Unconstrained And Articulated Human Body, Qin Wu, Guodong Guo

Faculty & Staff Scholarship

Gender recognition has many useful applications, ranging from business intelligence to image search and social activity analysis. Traditional research on gender recognition focuses on face images in a constrained environment. This paper proposes a method for gender recognition in articulated human body images acquired from an unconstrained environment in the real world. A systematic study of some critical issues in body-based gender recognition, such as which body parts are informative, how many body parts are needed to combine together, and what representations are good for articulated body-based gender recognition, is also presented. This paper also pursues data fusion schemes and …

Genotoxicity Of Multi-Walled Carbon Nanotubes At Occupationally Relevant Doses, Katelyn J. Siegrist, Steven H. Reynolds, Michael L. Kashon, David T. Lowry, Chenbo Dong, Ann F. Hubbs, Shih-Houng Young, Jeffrey L. Salisbury, Dale W. Porter, Stanley A. Benkovic, Michael Mccawley, Michael J. Keane, John T. Mastovich, Krisitin L. Bunker, Lorenzo G. Cena, Mark C. Sparrow, Jacqueline L. Sturgeon, Cerasela Z. Dinu, Linda M. Sargent

Faculty & Staff Scholarship

Carbon nanotubes are commercially-important products of nanotechnology; however, their low density and small size makes carbon nanotube respiratory exposures likely during their production or processing. We have previously shown mitotic spindle aberrations in cultured primary and immortalized human airway epithelial cells exposed to single-walled carbon nanotubes (SWCNT). In this study, we examined whether multi-walled carbon nanotubes (MWCNT) cause mitotic spindle damage in cultured cells at doses equivalent to 34 years of exposure at the NIOSH Recommended Exposure Limit (REL). MWCNT induced a dose responsive increase in disrupted centrosomes, abnormal mitotic spindles and aneuploid chromosome number 24 hours after exposure to …

Genome-Wide Association Study Of Periodontal Health Measured By Probing Depth In Adults Ages 18−49 Years, John R. Shaffer, Deborah E. Polk, Xiaojing Wang, Eleanor Feingold, Daniel E. Weeks, Myoung-Keun Lee, Karen T. Cuenco, Robert J. Weyant, Richard J. Crout, Daniel W. Mcneil, Mary L. Marazita

Faculty & Staff Scholarship

The etiology of chronic periodontitis clearly includes a heritable component. Our purpose was to perform a small exploratory genome-wide association study in adults ages 18–49 years to nominate genes associated with periodontal disease−related phenotypes for future consideration. Full-mouth periodontal pocket depth probing was performed on participants (N = 673), with affected status defined as two or more sextants with probing depths of 5.5 mm or greater. Two variations of this phenotype that differed in how missing teeth were treated were used in analysis. More than 1.2 million genetic markers across the genome were genotyped or imputed and tested for genetic …

Excess Risk Of Chronic Physical Conditions Associated With Depression And Anxiety, Rituparna Bhattacharya, Chan Shen, Usha Sambamoorthi

Faculty & Staff Scholarship

Background

Depression and anxiety have been reported to be associated with chronic physical conditions. We examined the excess risk of chronic physical conditions associated with depression and/or anxiety within a multivariate framework controlling for demographic and modifiable lifestyle risk factors.

Methods

We used a retrospective cross-sectional study design. Study participants were adults aged 22–64 years from 2007 and 2009 Medical Expenditure Panel Survey. We defined presence of depression-anxiety based on self-reported depression and anxiety and classified adults into 4 groups: 1) depression only; 2) anxiety only; 3) comorbid depression and anxiety 4) no depression and no anxiety. We included presence/absence …

The Splicing Regulator Ptbp2 Controls A Program Of Embryonic Splicing Required For Neuronal Maturation, Qin Li, Sika Zhaeng, Areum Han, Chia-Ho Lin, Peter Stoilov, Xiang-Dong Fu, Douglas L. Black

Faculty & Staff Scholarship

We show that the splicing regulator PTBP2 controls a genetic program essential for neuronal maturation. Depletion of PTBP2 in developing mouse cortex leads to degeneration of these tissues over the first three postnatal weeks, a time when the normal cortex expands and develops mature circuits. Cultured Ptbp2−/−neurons exhibit the same initial viability as wild type, with proper neurite outgrowth and marker expression. However, these mutant cells subsequently fail to mature and die after a week in culture. Transcriptome-wide analyses identify many exons that share a pattern of mis-regulation in the mutant brains, where isoforms normally found in adults …

Induction Of Wnt-Inducible Signaling Protein-1 Correlates With Invasive Breast Cancer Oncogenesis And Reduced Type 1 Cell-Mediated Cytotoxic Immunity: A Retrospective Study, David J. Klinke Ii

Faculty & Staff Scholarship

Innate and type 1 cell-mediated cytotoxic immunity function as important extracellular control mechanisms that maintain cellular homeostasis. Interleukin-12 (IL12) is an important cytokine that links innate immunity with type 1 cell-mediated cytotoxic immunity. We recently observed in vitro that tumor-derived Wnt-inducible signaling protein-1 (WISP1) exerts paracrine action to suppress IL12 signaling. The objective of this retrospective study was three fold: 1) to determine whether a gene signature associated with type 1 cell-mediated cytotoxic immunity was correlated with overall survival, 2) to determine whether WISP1 expression is increased in invasive breast cancer, and 3) to determine whether a gene signature consistent …

Military Personnel With Chronic Symptoms Following Blast Traumatic Brain Injury Have Differential Expression Of Neuronal Recovery And Epidermal Growth Factor Receptor Genes, Morgan Heinzelmann, Swarnalatha Y. Reddy, Louis M. French, Dan Wang, Hyunhwa Lee, Taura Barr, Tristin Baxter, Vincent Mysliwiec, Jessica Gill

Faculty & Staff Scholarship

Objective: Approximately one-quarter of military personnel who deployed to combat stations sustained one or more blast-related, closed-head injuries. Blast injuries result from the detonation of an explosive device. The mechanisms associated with blast exposure that give rise to traumatic brain injury (TBI), and place military personnel at high risk for chronic symptoms of post-concussive disorder (PCD), post-traumatic stress disorder (PTSD), and depression are not elucidated.

Methods: To investigate the mechanisms of persistent blast-related symptoms, we examined expression profiles of transcripts across the genome to determine the role of gene activity in chronic symptoms following blast-TBI. Active duty military personnel with …

De Novo Prediction Of Ptbp1 Binding And Splicing Targets Reveals Unexpected Features Of Its Rna Recognition And Function, Areum Han, Peter Stoilov, Anthony J. Linares, Yu Zhou, Xinag-Dong Fu, Douglas L. Black

Faculty & Staff Scholarship

The splicing regulator Polypyrimidine Tract Binding Protein (PTBP1) has four RNA binding domains that each binds a short pyrimidine element, allowing recognition of diverse pyrimidine-rich sequences. This variation makes it difficult to evaluate PTBP1 binding to particular sites based on sequence alone and thus to identify target RNAs. Conversely, transcriptome-wide binding assays such as CLIP identify many in vivo targets, but do not provide a quantitative assessment of binding and are informative only for the cells where the analysis is performed. A general method of predicting PTBP1 binding and possible targets in any cell type is needed. We developed computational …

Potential Impacts Of Extreme Salinity And Surface Temperature Events On Population Dynamics Of Black Drum, Pogonias Cromis, In The Upper Laguna Madre, Texas, Zachary T. Olsen

Gulf of Mexico Science

No abstract provided.

Presence Of Juvenile Lionfish In A Northern Gulf Of Mexico Nursery Habitat, Dorothy Byron, Kenneth L. Heck Jr., Mary A. Kennedy

Gulf of Mexico Science

No abstract provided.

College Catalog, 2014-2015, Graduate, Buffalo State College

Academic Catalogs, 1871-2018

No abstract provided.

College Catalog, 2014-2015, Buffalo State College

Academic Catalogs, 1871-2018

No abstract provided.

A High Throughput Mhc Ii Binding Assay For Quantitative Analysis Of Peptide Epitopes, Regina Salvat, Leonard Moise, Chris Bailey-Kellogg, Karl E. Griswold

Cell and Molecular Biology Faculty Publications

Biochemical assays with recombinant human MHC II molecules can provide rapid, quantitative insights into immunogenic epitope identification, deletion, or design^1,2. Here, a peptide-MHC II binding assay is scaled to 384-well format. The scaled down protocol reduces reagent costs by 75% and is higher throughput than previously described 96-well protocols^1,3-5. Specifically, the experimental design permits robust and reproducible analysis of up to 15 peptides against one MHC II allele per 384-well ELISA plate. Using a single liquid handling robot, this method allows one researcher to analyze approximately ninety test peptides in triplicate over a range of eight …

Escherichia Coli Pathotypes Occupy Distinct Niches In The Mouse Intestine, Jessica P. Meador, Matthew E. Caldwell, Paul S. Cohen, Tyrrell Conway

Cell and Molecular Biology Faculty Publications

Since the first step of the infection process is colonization of the host, it is important to understand how Escherichia coli pathogens successfully colonize the intestine. We previously showed that enterohemorrhagic O157:H7 strain E. coli EDL933 colonizes a niche in the streptomycin-treated mouse intestine that is distinct from that of human commensal strains, which explains how E. coli EDL933 overcomes colonization resistance imparted by some, but not all, commensal E. coli strains. Here we sought to determine if other E. coli pathogens use a similar strategy. We found that uropathogenic E. coli CFT073 and enteropathogenic E. coli E2348/69 occupy intestinal …

Dietary Supplement Labeling: Cognitive Biases, Market Manipulation & Consumer Choice, Michael Mccann

Law Faculty Scholarship

There exists increasing concern that the Dietary Supplements Health and Education Act (DSHEA) has proven ineffective. Much of the concern regards the disparity in legislative treatment between dietary supplements, foods, and pharmaceutical drugs. Namely, while pharmaceutical drugs must undergo years of costly pre-market testing, most supplements, like foods, can immediately enter the market, and only after repeated instances of adverse reactions can the Food and Drug Administration (FDA) remove them. Such a framework appears to belie both consumer expectations and marketing strategies, as supplements tend to be most perceived for their apparent medicinal qualities. This philosophy of waiting for a …

Contraceptive Sabotage, Leah A. Plunkett

Law Faculty Scholarship

This Article responds to the alarm recently sounded by the American College of Obstetricians and Gynecologists over “birth control sabotage”—the “active interference [by one partner] with [the other] partner’s contraceptive methods in an attempt to promote pregnancy.” Currently, sabotage is not a crime, and existing categories of criminal offenses fail to capture the essence of the injury it does to victims. This Article argues that sabotage should be a separate crime—but only when perpetrated against those partners who can and do get pregnant as a result of having sabotaged sex. Using the principle of self-possession—understood as a person’s basic right …