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National Climate Indicators System Report, National Climate Assessment And Development Advisory Committee

Numerical Terradynamic Simulation Group Publications

The National Climate Assessment (NCA), a component of the U.S. Global Change Research Program (USGCRP), is designed to produce periodic scientific assessments of the vulnerability of important sectors in the U.S. to climate change and variability, and to report on response strategies for responding to and coping with change. An important feature of the NCA is to develop climate-relevant information for use by a wide variety of stakeholders in the public and private sectors and in the scientific community. The development of a national system of indicators is an essential feature of such information, and provides a foundation for assessing …

Repair Of Oxidative Dna Damage And Cancer: Recent Progress In Dna Base Excision Repair, Timothy L. Scott, Suganya Rangaswamy, Christina A. Wicker, Tadahide Izumi

Toxicology and Cancer Biology Faculty Publications

SIGNIFICANCE: Reactive oxygen species (ROS) are generated by exogenous and environmental genotoxins, but also arise from mitochondria as byproducts of respiration in the body. ROS generate DNA damage of which pathological consequence, including cancer is well established. Research efforts are intense to understand the mechanism of DNA base excision repair, the primary mechanism to protect cells from genotoxicity caused by ROS.

RECENT ADVANCES: In addition to the notion that oxidative DNA damage causes transformation of cells, recent studies have revealed how the mitochondrial deficiencies and ROS generation alter cell growth during the cancer transformation.

CRITICAL ISSUES: The emphasis of this …

Ether Bridge Formation In Loline Alkaloid Biosynthesis, Juan Pan, Minakshi Bhardwaj, Jerome Ralph Faulkner, Padmaja Nagabhyru, Nikki D. Charlton, Richard M. Higashi, Anne-Frances Miller, Carolyn A Young, Robert B. Grossman, Christopher L. Schardl

Toxicology and Cancer Biology Faculty Publications

Lolines are potent insecticidal agents produced by endophytic fungi of cool-season grasses. These alkaloids are composed of a pyrrolizidine ring system and an uncommon ether bridge linking carbons 2 and 7. Previous results indicated that 1-aminopyrrolizidine was a pathway intermediate. We used RNA interference to knock down expression of lolO, resulting in the accumulation of an alkaloid identified as exo-1-acetamidopyrrolizidine based on high-resolution MS and NMR. Genomes of endophytes differing in alkaloid profiles were sequenced, revealing that those with mutated lolO accumulated exo-1-acetamidopyrrolizidine but no lolines. Heterologous expression of wild-type lolO complemented a lolO mutant, resulting in …

Riverine Macrosystems Ecology: Sensitivity, Resistance, And Resilience Of Whole River Basins With Human Alterations, Kevin E. Mccluney, N Leroy Poff, Margaret A. Palmer, James H. Thorp, Geoffrey C. Poole, Bradley S. Williams, Michael R. Williams, Jill S. Baron

Biological Sciences Faculty Publications

Riverine macrosystems are described here as watershed-scale networks of connected and interacting riverine and upland habitat patches. Such systems are driven by variable responses of nutrients and organisms to a suite of global and regional factors (eg climate, human social systems) interacting with finer-scale variations in geology, topography, and human modifications. We hypothesize that spatial heterogeneity, connectivity, and asynchrony among these patches regulate ecological dynamics of whole networks, altering system sensitivity, resistance, and resilience. Long-distance connections between patches may be particularly important in riverine macrosystems, shaping fundamental system properties. Furthermore, the type, extent, intensity, and spatial configuration of human activities …

Effect Of Aquatic Immersion On Static Balance, Talin Louder, Eadric Bressel, Matt Baldwin, Dennis G. Dolny, Richard Gordin, Andrew Miller

International Journal of Aquatic Research and Education

No abstract provided.

Pipeline Revision: A Call To Change, Roni Reiter-Palmon, Pablo P. L. Tinio

Psychology Faculty Publications

Comments on the original article by Matthew Makel (see record 2014-06823-002) which raises an important concern regarding selfcorrection in science. While the notion that science should be self-correcting, and is failing, is not new, recent events in both social and biological sciences have led to a renewed call to develop approaches that would allow for self-correction ( Economist, 2013). Makel addresses a number of issues that hinder self-correction through replications and suggests a number of solutions. In this commentary, we as the editors of Psychology of Aesthetics, Creativity, and the Arts (PACA), would like to offer our perspective. …

Navigating The Roles Of Leadership: Mentors Perspectives On Teacher Leadership, Saundra Shillingstad, Sheryl Mcglamery, Barbara Davis, Carol Gilles

Teacher Education Faculty Proceedings & Presentations

The qualitative study described in this paper began as a collaborative project between three Universities offering comprehensive induction programs to first year teachers. Fourteen teacher mentors were selected to participate in this case study of teacher leaders and leadership. Three of the fourteen teacher leaders were selected to participate in a special case study of their leadership development. The researchers found their leadership qualities to be exemplary and wanted to explore in more depth the three teacher leaders and how they developed and shared their skills as leaders with their mentees and colleagues. The three participants represent perspectives from three …

An Abundant Dysfunctional Apolipoprotein A1 In Human Atheroma, Ying Huang, Joseph A. Didonato, Bruce S. Levison, Dave Schmitt, Lin Li, Yuping Wu, Jennifer Buffa, Timothy Kim, Gary S. Gerstenecker, Xiaodong Gu, Chandra S. Kadiyala, Zeneng Wang, Miranda K. Culley, Jennie E. Hazen, Anthony J. Didonato, Xiaoming Fu, Stela Z. Berisha, Daoquan Peng, Truc T. Nguyen, Shaohong Liang, Chia-Chi Chuang, Leslie Cho, Edward F. Plow, Paul L. Fox, Valentin Gogonea, W.H. Wilson Tang, John S. Parks, Edward A. Fisher, Jonathan D. Smith, Stanley L. Hazen

Chemistry Faculty Publications

Recent studies have indicated that high-density lipoproteins (HDLs) and their major structural protein, apolipoprotein A1 (apoA1), recovered from human atheroma are dysfunctional and are extensively oxidized by myeloperoxidase (MPO). In vitro oxidation of either apoA1 or HDL particles by MPO impairs their cholesterol acceptor function. Here, using phage display affinity maturation, we developed a high-affinity monoclonal antibody that specifically recognizes both apoA1 and HDL that have been modified by the MPO-H2O2-Cl− system. An oxindolyl alanine (2-OH-Trp) moiety at Trp72 of apoA1 is the immunogenic epitope. Mutagenesis studies confirmed a critical role for apoA1 Trp72 in MPO-mediated inhibition of the ATP-binding …

February 2014 News Releases, University Of Montana--Missoula. Office Of University Relations

University of Montana News Releases, 1928, 1956-present

No abstract provided.

Fetal Baboon Sex-Specific Outcomes In Adipocyte Differentiation At 0.9 Gestation In Response To Moderate Maternal Nutrient Reduction, Y. D. Tchoukalova, R. Krishnapuram, U. A. White, D. Burk, X. Fang, M. J. Nijland, P. W. Nathanielsz

Faculty Publications

Objective:To investigate in vitro adipocyte differentiation in baboon fetuses in response to reduced maternal nutrition.Design:Cross-sectional comparison of adipocyte differentiation in normally grown fetuses and fetuses of pregnant baboons fed 70% of the control global diet from 30 days of pregnancy to term.Subjects:The subjects comprised control (CTR) fetuses (five female and five male) of mothers fed ad libitum and fetuses of mothers fed 70% of the global diet consumed by CTR (maternal nutrient reduction (MNR), five female and five male fetuses). The expression of genes/proteins involved in adipogenesis (PPARγ, FABP4 and adiponectin) and brown adipose tissue development (UCP1, TBX15 and COXIV) …

Contributions Of Adipose Tissue Architectural And Tensile Properties Toward Defining Healthy And Unhealthy Obesity, Denise E. Lackey, David H. Burk, Mohamed R. Ali, Rouzbeh Mostaedi, William H. Smith, Jiyoung Park, Philipp E. Scherer, Shundra A. Seay, Colin S. Mccoin, Paolo Bonaldo, Sean H. Adams

Faculty Publications

The extracellular matrix (ECM) plays an important role in the maintenance of white adipose tissue (WAT) architecture and function, and proper ECM remodeling is critical to support WAT malleability to accomodate changes in energy storage needs. Obesity and adipocyte hypertrophy place a strain on the ECM remodeling machinery, which may promote disordered ECM and altered tissue integrity and could promote proinflammatory and cell stress signals. To explore these questions, new methods were developed to quantify omental and subcutaneous WAT tensile strength and WAT collagen content by three-dimensional confocal imaging, using collagen VI knockout mice as a methods validation tool. These …

Farming The Slums: Using Eminent Domain And Urban Agriculture To Rebuild Baltimore's Blighted Neighborhoods, Keith Buzby

William & Mary Environmental Law and Policy Review

No abstract provided.

A Statute Stripped Of Its Sting: Colony Collapse Disorder And The Epa's "Imminent Hazard", Tobias Eisenlohr

William & Mary Environmental Law and Policy Review

No abstract provided.

Mckillop Library Newsbrief Feb 2014

McKillop Library Newsbrief

No abstract provided.

Strengthening Interactions Between Statisticians And Collaborators: Objectives And Sample Sizes, Emily Van Meter, Richard Charnigo

Biostatistics Faculty Publications

No abstract provided.

How Clinical Education Builds Bridges With Villages For A Global Law School In India, Priya S. Gupta, Kudrat Dev, Meher Dev, Kirti Rana

Journal of Legal Education

No abstract provided.

Quantifying The Statistical Impact Of Grappa In Fcmri Data With A Real-Valued Isomorphism, Iain P. Bruce, Daniel B. Rowe

Mathematics, Statistics and Computer Science Faculty Research and Publications

The interpolation of missing spatial frequencies through the generalized auto-calibrating partially parallel acquisitions (GRAPPA) parallel magnetic resonance imaging (MRI) model implies a correlation is induced between the acquired and reconstructed frequency measurements. As the parallel image reconstruction algorithms in many medical MRI scanners are based on the GRAPPA model, this study aims to quantify the statistical implications that the GRAPPA model has in functional connectivity studies. The linear mathematical framework derived in the work of Rowe , 2007, is adapted to represent the complex-valued GRAPPA image reconstruction operation in terms of a real-valued isomorphism, and a statistical analysis is performed …

Disease And Predation: Sorting Out Causes Of A Bighorn Sheep (Ovis Canadensis) Decline, Joshua B. Smith, Jonathan A. Jenks, Troy Grovenburg, Robert W. Klaver

Natural Resource Management Faculty Publications

Estimating survival and documenting causes and timing of mortality events in neonate bighorn sheep (Ovis canadensis) improves understanding of population ecology and factors influencing recruitment. During 2010–2012, we captured and radiocollared 74 neonates in the Black Hills, South Dakota, of which 95% (70) died before 52 weeks of age. Pneumonia (36%) was the leading cause of mortality followed by predation (30%). We used known fate analysis in Program MARK to estimate weekly survival rates and investigate the influence of intrinsic variables on 52-week survival. Model {S1 wk, 2–8 wks, .8 wks} had the lowest AICc (Akaike’s Information Criterion corrected for …

An Abundant Dysfunctional Apolipoprotein A1 In Human Atheroma, Ying Huang, Joseph A. Didonato, Bruce S. Levison, Dave Schmitt, Lin Li, Yuping Wu, Jennifer Buffa, Timothy Kim, Gary S. Gerstenecker, Xiaodong Gu, Chandra S. Kadiyala, Zeneng Wang, Miranda K. Culley, Jennie E. Hazen, Anthony J. Didonato, Xiaoming Fu, Stela Z. Berisha, Daoquan Peng, Truc T. Nguyen, Shaohong Liang, Chia-Chi Chuang, Leslie Cho, Edward F. Plow, Paul L. Fox, Valentin Gogonea, W.H. Wilson Tang, John S. Parks, Edward A. Fisher, Jonathan D. Smith, Stanley L. Hazen

Mathematics and Statistics Faculty Publications

Recent studies have indicated that high-density lipoproteins (HDLs) and their major structural protein, apolipoprotein A1 (apoA1), recovered from human atheroma are dysfunctional and are extensively oxidized by myeloperoxidase (MPO). In vitro oxidation of either apoA1 or HDL particles by MPO impairs their cholesterol acceptor function. Here, using phage display affinity maturation, we developed a high-affinity monoclonal antibody that specifically recognizes both apoA1 and HDL that have been modified by the MPO-H2O2-Cl− system. An oxindolyl alanine (2-OH-Trp) moiety at Trp72 of apoA1 is the immunogenic epitope. Mutagenesis studies confirmed a critical role for apoA1 Trp72 in MPO-mediated inhibition of the ATP-binding …

A Novel In Vivo Model For Evaluating Functional Restoration Of A Tissue-Engineered Salivary Gland., Swati Pradhan-Bhatt, Daniel A Harrington, Randall L Duncan, Mary C Farach-Carson, Xinqiao Jia, Robert L Witt

Department of Otolaryngology - Head and Neck Surgery Faculty Papers

OBJECTIVES/HYPOTHESIS: To create a novel model for development of a tissue-engineered salivary gland from human salivary gland cells that retains progenitor cell markers useful for treatment of radiation-induced xerostomia.

STUDY DESIGN: A three-dimensional (3D) hyaluronic acid (HA)-based hydrogel scaffold was used to encapsulate primary human salivary gland cells and to obtain organized acini-like spheroids. Hydrogels were implanted into rat models, and cell viability and receptor expression were evaluated.

METHODS: A parotid gland surgical resection model for xenografting was developed. Salivary cells loaded in HA hydrogels formed spheroids and in vitro were implanted in the three-fourths resected parotid bed of athymic …

The Chromatin Architectural Proteins Hmgd1 And H1 Bind Reciprocally And Have Opposite Effects On Chromatin Structure And Gene Regulation, Narasimharao Nalabothula, Graham Mcvicker, John Maiorano, Rebecca Martin, Jonathan K. Pritchard, Yvonne N. Fondufe-Mittendorf

Molecular and Cellular Biochemistry Faculty Publications

BACKGROUND: Chromatin architectural proteins interact with nucleosomes to modulate chromatin accessibility and higher-order chromatin structure. While these proteins are almost certainly important for gene regulation they have been studied far less than the core histone proteins.

RESULTS: Here we describe the genomic distributions and functional roles of two chromatin architectural proteins: histone H1 and the high mobility group protein HMGD1 in Drosophila S2 cells. Using ChIP-seq, biochemical and gene specific approaches, we find that HMGD1 binds to highly accessible regulatory chromatin and active promoters. In contrast, H1 is primarily associated with heterochromatic regions marked with repressive histone marks. We find …

The Ten Most Common Mistakes In Using No-Till, Earl Creech, Phil Rasmussen, Robert Newhall

All Current Publications

Interest in no-till (NT) crop production practices has increased greatly among Utah farmers and ranchers in recent years. This publication highlights 10 common mistakes that are often made by producers that are new to NT.

A Distinct Type Of Heterochromatin At The Telomeric Region Of The Drosophila Melanogaster Y Chromosome, Ruth Nan, Sidney Wang, Maria Accardo, Monica F. Sentmanat, Patrizio Dimitri, Sarah C.R. Elgin

Biology Faculty Research

Heterochromatin assembly and its associated phenotype, position effect variegation (PEV), provide an informative system to study chromatin structure and genome packaging. In the fruit fly Drosophila melanogaster, the Y chromosome is entirely heterochromatic in all cell types except the male germline; as such, Y chromosome dosage is a potent modifier of PEV. However, neither Y heterochromatin composition, nor its assembly, has been carefully studied. Here, we report the mapping and characterization of eight reporter lines that show male-specific PEV. In all eight cases, the reporter insertion sites lie in the telomeric transposon array (HeT-A and TART-B2 homologous repeats) of the …

Integrating Water Exclusion Theory Into Β Contacts To Predict Binding Free Energy Changes And Binding Hot Spots, Qian Liu, Steven C. H. Hoi, Chee Keong Kwoh, Limsoon Wong, Jinyan Li

Research Collection School Of Computing and Information Systems

Binding free energy and binding hot spots at protein-protein interfaces are two important research areas for understanding protein interactions. Computational methods have been developed previously for accurate prediction of binding free energy change upon mutation for interfacial residues. However, a large number of interrupted and unimportant atomic contacts are used in the training phase which caused accuracy loss. Results: This work proposes a new method, β ACV ASA , to predict the change of binding free energy after alanine mutations. β ACV ASA integrates accessible surface area (ASA) and our newly defined β contacts together into an atomic contact vector …

Managing The Innovation Supply Chain To Maximize Personalized Medicine., Scott A. Waldman, Andre Terzic

Department of Pharmacology and Experimental Therapeutics Faculty Papers

Personalized medicine epitomizes an evolving model of care tailored to the individual patient. This emerging paradigm harnesses radical technological advances to define each patient's molecular characteristics and decipher his or her unique pathophysiological processes. Translated into individualized algorithms, personalized medicine aims to predict, prevent, and cure disease without producing therapeutic adverse events. Although the transformative power of personalized medicine is generally recognized by physicians, patients, and payers, the complexity of translating discoveries into new modalities that transform health care is less appreciated. We often consider the flow of innovation and technology along a continuum of discovery, development, regulation, and application …

Corticosterone Administration Up-Regulated Expression Of Norepinephrine Transporter And Dopamine Β-Hydroxylase In Rat Locus Coeruleus And Its Terminal Regions, Yan Fan, Ping Ping Chen, Ying Li, Kui Cui, Daniel M. Noel, Elizabeth D. Cummins, Daniel J. Peterson, Russell W. Brown, Meng-Yang Zhu

ETSU Faculty Works

Stress has been reported to activate the locus coeruleus (LC)-noradrenergic system. In this study, corticosterone (CORT) was orally administrated to rats for 21 days to mimic stress status. In situ hybridization measurements showed that CORT ingestion significantly increased mRNA levels of norepinephrine transporter (NET) and dopamine β-hydroxylase (DBH) in the LC region. Immunofluorescence staining and western blotting revealed that CORT treatment also increased protein levels of NET and DBH in the LC, as well as NET protein levels in the hippocampus, the frontal cortex and the amygdala. However, CORT-induced increase in DBH protein levels only appeared in the hippocampus and …

Coaching: What Business And Social Researchers Need To Know About It, Raymond L. Forbes

All Faculty and Staff Scholarship

This paper explores the historical and current world of organizational coaching. Coaching is offered as a means of assistance to aid organizational leaders in effectively responding to the stressful external and internal demands associated with their positions or ones to which they aspire. Coaching is also discussed as a vehicle for improving individual and team performance as well as for actualizing a leader’s inherent potential. This work draws heavily upon current literature and practice in both the leadership and coaching fields. It also provides a review of relevant theory, contrasts the roles of leader and manager, defines executive coaching, and …

Robust Quasi-Lpv Controller Design Via Integral Quadratic Constraint Analysis, James C. Kempsell Jr.

Theses

Reduced cost of sensors and increased computing power is enabling

the development and implementation of control systems that can

simultaneously regulate multiple variables and handle conflicting

objectives while maintaining stringent performance objectives. To

make this a reality, practical analysis and design tools must be developed

that allow the designer to trade-off conflicting objectives and

guarantee performance in the presence of uncertain system dynamics,

an uncertain environment, and over a wide range of operating

conditions. As a first step towards this goal, we organize and streamline

a promising robust control approach, Robust Linear Parameter

Varying control, which integrates three fields of …

A Prospective, Multicenter, Phase I Matched-Comparison Group Trial Of Safety, Pharmacokinetics, And Preliminary Efficacy Of Riluzole In Patients With Traumatic Spinal Cord Injury., Robert G Grossman, Michael G Fehlings, Ralph F Frankowski, Keith D Burau, Diana S L Chow, Charles Tator, Angela Teng, Elizabeth G Toups, James Harrop, Bizhan Aarabi, Christopher I Shaffrey, Michele M Johnson, Susan J Harkema, Maxwell Boakye, James D Guest, Jefferson R Wilson

Department of Neurosurgery Faculty Papers

A prospective, multicenter phase I trial was undertaken by the North American Clinical Trials Network (NACTN) to investigate the pharmacokinetics and safety of, as well as obtain pilot data on, the effects of riluzole on neurological outcome in acute spinal cord injury (SCI). Thirty-six patients, with ASIA impairment grades A-C (28 cervical and 8 thoracic) were enrolled at 6 NACTN sites between April 2010 and June 2011. Patients received 50 mg of riluzole PO/NG twice-daily, within 12 h of SCI, for 14 days. Peak and trough plasma concentrations were quantified on days 3 and 14. Peak plasma concentration (Cmax) and …

Targeting Fibroblast Activation Protein In Tumor Stroma With Chimeric Antigen Receptor T Cells Can Inhibit Tumor Growth And Augment Host Immunity Without Severe Toxicity., Liang-Chuan S. Wang, Albert Lo, John Scholler, Jing Sun, Rajrupa S. Majumdar, Veena Kapoor, Michael Antzis, Cody E. Cotner, Laura A. Johnson, Amy C. Durham, Charalambos C. Solomides, Md, Carl H. June, Ellen Puré, Steven M. Albelda

Department of Pathology, Anatomy, and Cell Biology Faculty Papers

The majority of chimeric antigen receptor (CAR) T-cell research has focused on attacking cancer cells. Here, we show that targeting the tumor-promoting, nontransformed stromal cells using CAR T cells may offer several advantages. We developed a retroviral CAR construct specific for the mouse fibroblast activation protein (FAP), comprising a single-chain Fv FAP [monoclonal antibody (mAb) 73.3] with the CD8α hinge and transmembrane regions, and the human CD3ζ and 4-1BB activation domains. The transduced muFAP-CAR mouse T cells secreted IFN-γ and killed FAP-expressing 3T3 target cells specifically. Adoptively transferred 73.3-FAP-CAR mouse T cells selectively reduced FAP(hi) stromal cells and inhibited the …