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Exposure To Multiple Parasites Is Associated With The Prevalence Of Active Convulsive Epilepsy In Sub-Saharan Africa, Gathoni Kamuyu, Christian Bottomley, James Mageto, Brett Lowe, Patricia Wilkins, John C. Noh, Thomas B. Nutman, Anthony Ngugi, Rachael Odhiambo, Ryan Wagner, Angelina Kakooza-Mwesige, Seth Owusu-Agyei, Kenneth Ae-Ngibise, Honorati Masanja, Faith Osier, Peter Odermatt, Charles Newton

Family Medicine, East Africa

Background: Epilepsy is common in developing countries, and it is often associated with parasitic infections. We investigated the relationship between exposure to parasitic infections, particularly multiple infections and active convulsive epilepsy (ACE), in five sites across sub-Saharan Africa.

Methods and Findings: A case-control design that matched on age and location was used. Blood samples were collected from 986 prevalent cases and 1,313 age-matched community controls and tested for presence of antibodies to Onchocerca volvulus, Toxocara canis, Toxoplasma gondii, Plasmodium falciparum, Taenia solium and HIV. Exposure (seropositivity) to Onchocerca volvulus (OR = 1.98; 95%CI: 1.52-2.58, p

Conclusion: This study investigates how …

Premature Mortality In Active Convulsive Epilepsy In Rural Kenya: Causes And Associated Factors, Anthony Ngugi, Christian Bottomley, Gregory Fegan, Eddie Chengo, Rachael Odhiambo, Evasius Bauni, Brian Neville, Immo Kleinschmidt, Josemir W. Sander, Charles R. Newton

Family Medicine, East Africa

Objective: We estimated premature mortality and identified causes of death and associated factors in people with active convulsive epilepsy (ACE) in rural Kenya.

Methods: In this prospective population-based study, people with ACE were identified in a crosssectional survey and followed up regularly for 3 years, during which information on deaths and associated factors was collected. We used a validated verbal autopsy tool to establish putative causes of death. Age-specific rate ratios and standardized mortality ratios were estimated. Poisson regression was used to identify mortality risk factors.

Results: There were 61 deaths among 754 people with ACE, yielding a rate of …

Epigenetic Changes After Prenatal Environmental Tobacco Smoke Exposure And Asthma, Sonja Christensen

Graduate Student Theses, Dissertations, & Professional Papers

Allergic asthma affects over 300 million people worldwide. Multiple factors have been shown to alter an individuals' susceptibility to this allergic airway disease such as exposure to polycyclic aromatic hydrocarbons (PAHs), endotoxin, and environmental tobacco smoke (ETS). Prenatal exposure to these contaminants, especially ETS, has been shown to increase risk for an individuals’ asthma risk, however, the mechanism by which this happens is still unknown. Epigenetic alterations, particularly DNA methylation patterns, have been introduced as a proposed mechanism. The goals of this project were to use a house dust mite (HDM) murine model of asthma to determine the methylation alterations …

Lowveld Practices In Mahenye, Zimbabwe: A Critical Analysis Of Resilience In A Marginalized Southern African Community, Scott Byington

Graduate Student Theses, Dissertations, & Professional Papers

The concept of resilience has been developed by scholars in the social-ecological systems resilience and community resilience/disaster literature. While initially a descriptive model, the framework of resilience has since integrated both literatures and become a prescriptive tool for sustainable development initiatives. Political ecologists critically argue that the current conceptualization of resilience is problematic given resilience scholars’ lack of engagement with situational social complexities related to power and knowledge. They also argue that mainstream resilience literature is devoid of cultural, historical and political analysis. As such, individuals and communities at the margins of power may vulnerable to the application of resilience …

Characterization Of Rift Valley Fever Virus Nucleocapsid Protein-Rna Binding Interactions And Development Of A High-Throughput Screening Assay For Identification Of N-Rna Binding Inhibitors, Mary Lee Ellenbecker

Graduate Student Theses, Dissertations, & Professional Papers

Rift Valley fever virus (RVFV) is an emerging infectious pathogen that causes severe disease in humans and livestock and has the potential for global spread. Currently, there is no treatment for RVFV infection and there is no proven safe and effective vaccine. Inhibition of RNA binding to RVFV nucleocapsid (N) protein represents an innovative antiviral therapeutic strategy because several essential steps in the RVFV replication cycle involve N binding to viral RNA. The overall goals of our research are to better understand how RVFV N protein interacts with RNA, to develop a high-throughput drug screening assay for identification of compounds …

Demographic And Epidemiological Changes On The Flathead Reservation 1887-1935, Christina Joy Heiner

Graduate Student Theses, Dissertations, & Professional Papers

This dissertation examines the patterns of health among the Salish, Pend d’Oreille, and Kootenai tribes living on the Flathead Reservation in the late 19th and early 20th century. Previous research on early Native American demographic and epidemiological patterns has essentially focused on patterns following European contact to the pre-reservation era, ignoring that Native populations continued to struggle with ill-health and disease under the control of the Bureau of Indian Affairs. This research found while large infectious disease epidemics did not occur after the 1900s, infectious diseases such as tuberculosis and pneumonia were the leading causes of death for all ages …

Light Stimulates The Mouse Adrenal Through A Retinohypothalamic Pathway Independent Of An Effect On The Clock In The Suprachiasmatic Nucleus, Silke Kiessling, Patricia J. Sollars, Gary E. Pickard

School of Veterinary and Biomedical Sciences: Faculty Publications

The brain’s master circadian pacemaker resides within the hypothalamic suprachiasmatic nucleus (SCN). SCN clock neurons are entrained to the day/night cycle via the retinohypothalamic tract and the SCN provides temporal information to the central nervous system and to peripheral organs that function as secondary oscillators. The SCN clock-cell network is thought to be the hypothalamic link between the retina and descending autonomic circuits to peripheral organs such as the adrenal gland, thereby entraining those organs to the day/night cycle. However, there are at least three different routes or mechanisms by which retinal signals transmitted to the hypothalamus may be conveyed …

Dens Agenesis And Cervical Vertebral Malformation In A Labrador Retriever Puppy, Elisa Salas, Henry E. Cerny, Seth P. Harris

School of Veterinary and Biomedical Sciences: Faculty Publications

Cervical vertebral malformations can cause acute or progressive pain, paralysis, and potentially death. Herein we present a case report of dens agenesis; cervical vertebral malformations of C1, C2, C6, and C7; subluxation of C1 and C2 and C4 and C5; and associated degenerative myelopathy in a 5-month-old female Labrador Retriever puppy. We additionally review current knowledge regarding pathogenesis and treatment.

The Anthropology Of Plague: Insights From Bioarcheological Analyses Of Epidemic Cemeteries, Sharon Dewitte

Faculty Publications

Most research on historic plague has relied on documentary evidence, but recently researchers have examined the remains of plague victims to produce a deeper understanding of the disease. Bioarcheological analysis allows the skeletal remains of epidemic victims to bear witness to the contexts of their deaths. This is important for our understanding of the experiences of the vast majority of people who lived in the past, who are not typically included in the historical record. This paper summarizes bioarcheological research on plague, primarily investigations of the Black Death in London (1349–50), emphasizing what anthropology uniquely contributes to plague studies.

Role Of Scavenger Receptor Class B Type I In Thymopoiesis, Zhong Zheng

Theses and Dissertations--Nutritional Sciences

T cells, which constitute an essential arm in the adaptive immunity, complete their development in the thymus through a process called thymopoiesis. However, thymic involution can be induced by a couple of factors, which impairs T cell functions and is slow to recover. Therefore, understanding how thymopoiesis is regulated may lead effort to accelerate thymic recovery and improve immune functions in thymocyte-depleted patients. In this project, we identified scavenger receptor BI (SR-BI), a high density lipoprotein (HDL) receptor, as a novel modulator in thymopoiesis. In mice, absence of SR-BI causes a significant reduction in thymus size after puberty and a …

The Role Of Scavenger Receptor Class B Type I-Regulated Inducible Glucocorticoids In Sepsis, Junting Ai

Theses and Dissertations--Nutritional Sciences

Sepsis claims over 215,000 lives in the US annually. Inducible glucocorticoids (iGC) is produced during sepsis. However, the precise effects of iGC in sepsis remain unclear due to a lack of appropriate animal models. Glucocorticoid (GC) insufficiency is associated with a marked increase in mortality and occurs in 60% of severe septic patients. Yet the conclusion of GC therapy on septic patients is still controversial.

Scavenger receptor class B type I (SR-BI) in the adrenal mediates the selective uptake of cholesteryl ester from lipoproteins for GC synthesis. SR-BI-/- mice completely lack iGC during sepsis and are highly susceptible to septic …

Classification Of Epidemic Community-Acquired Methicillin-Resistant Staphylococcus Aureus By Anatomical Site Of Isolation, Jill C. Roberts

Environmental and Occupational Health Faculty Publications

Methicillin-resistant Staphylococcus aureus contributes significantly to cost, morbidity, and mortality due to infectious disease. We surveyed community-associated MRSA isolates to determine which strains were present within anatomical sites of interest. The most likely sources of MRSA among anatomic sites swabbed were wounds followed by the nasal cavity. The USA 300 MRSA strain was most commonly isolated among wound infections while nasal swabs largely yielded USA 100 MRSA. The frequency of isolation of USA 100 amongst community-associated strains is clinically significant as this strain is often correlated with invasive disease, exhibits broad antibiotic resistance, and has been considered to be hospital …

A Paradox In Development: Exploring The Obesity Pandemic In Latin America, Laila G. Heid

CMC Senior Theses

The purpose of this paper is to explain the obesity pandemic in Latin America and identify the factors of development contributing to the pandemic. The paper uses the framework of the nutrition transition as presented by Barry Popkin to trace consumption patterns and changes in dietary habits in the region. The paper looks at three case studies: Mexico and Chile, two countries with high obesity rates, and Peru, the country with the lowest obesity rate in South America. This comparative framework is intended to determine which conditions are necessary for obesity, which conditions are sufficient for obesity, and any conditions …

Relative Importance Of Host Environment, Transmission Potential And Host Phylogeny To The Structure Of Parasite Metacommunities, Tad Dallas, Steven J. Presley

Faculty Publications

Identification of mechanisms that shape parasite community and metacommunity structures have important implications to host health, disease transmission, and the understanding of community assembly in general. Using a long-term dataset on parasites from desert rodents, we examined the relative contributions of host traits that represent important aspects of parasite environment, transmission probability between host species, and host phylogeny to the structure of a parasite metacommunity as well as for taxonomically restricted parasite metacommunities (coccidians, ectoparasites and helminths). This was done using a combination of metacommunity analysis and variance partitioning based on canonical correspondence analysis. Coccidian and ectoparasite metacommunities did not …

Nitrate Enrichment Alters A Daphnia-Microparasite Interaction Through Multiple Pathways, Tad Dallas, John M. Drake

Faculty Publications

© 2013 The Authors. Ecology and Evolution published by John Wiley & Sons Ltd. Nutrient pollution has the potential to alter many ecological interactions, including host-parasite relationships. One of the largest sources of nutrient pollution comes from anthropogenic alteration of the nitrogen (N) cycle, specifically the increased rate of nitrate (NO3 -N) deposition to aquatic environments, potentially altering host-parasite relationships. This study aimed to assess the mechanisms through which nitrate may impact host-pathogen relationships using a fungal pathogen (Metschnikowia bicuspidata) parasitic to crustacean zooplankton (Daphnia dentifera) as a tractable model system. First, the influence of nitrate on host population dynamics …

Marrow Of Tragedy: The Health Crisis Of The American Civil War, Rea Andrew Redd

Civil War Book Review

The Issue of Public Health in a Time of War

At the American Sociological Association’s annual meeting during August, 1986, Maris A. Vinovskis posed a question: “Have social historians lost the Civil War?" He stated that indeed the “social historians of the nineteenth century appear to hav....

Ruminant Self-Medication Against Gastrointestinal Nematodes: Evidence, Mechanism, And Origins Ẫ†, Juan J. Villalba, James Miller, Eugene D. Ungar, Serge Y. Landau, John Glendinning

Faculty Publications

Gastrointestinal helminths challenge ruminants in ways that reduce their fitness. In turn, ruminants have evolved physiological and behavioral adaptations that counteract this challenge. Ruminants display anorexia and avoidance behaviors, which tend to reduce the incidence of parasitism. In addition, ruminants appear to learn to self-medicate against gastrointestinal parasites by increasing consumption of plant secondary compounds with antiparasitic actions. This selective feeding improves health and fitness. Here, we review the evidence for self-medication in ruminants, propose a hypothesis to explain self-medicative behaviors (based on post-ingestive consequences), and discuss mechanisms (e.g., enhanced neophilia, social transmission) that may underlie the ontogeny and spread …

Herpes Simplex Virus 1 Protein Ul37 Interacts With Viral Glycoprotein Gk And Membrane Protein Ul20 And Functions In Cytoplasmic Virion Envelopment, Nithya Jambunathan, Dmitry Chouljenko, Prashant Desai, Anu Susan Charles, Ramesh Subramanian, Vladimir N. Chouljenko, Konstantin G. Kousoulas

Faculty Publications

We have shown that glycoprotein K (gK) and its interacting partner, the UL20 protein, play crucial roles in virion envelopment. Specifically, virions lacking either gK or UL20 fail to acquire an envelope, thus causing accumulation of capsids in the cytoplasm of infected cells. The herpes simplex virus 1 (HSV-1) UL37 protein has also been implicated in cytoplasmic virion envelopment. To further investigate the role of UL37 in virion envelopment, the recombinant virus DC480 was constructed by insertion of a 12-amino-acid protein C (protC) epitope tag within the UL37 amino acid sequence immediately after amino acid 480. The DC480 mutant virus …

Computational Redesign Of Bacterial Biotin Carboxylase Inhibitors Using Structure-Based Virtual Screening Of Combinatorial Libraries, Michal Brylinski, Grover L. Waldrop

Faculty Publications

As the spread of antibiotic resistant bacteria steadily increases, there is an urgent need for new antibacterial agents. Because fatty acid synthesis is only used for membrane biogenesis in bacteria, the enzymes in this pathway are attractive targets for antibacterial agent development. Acetyl-CoA carboxylase catalyzes the committed and regulated step in fatty acid synthesis. In bacteria, the enzyme is composed of three distinct protein components: biotin carboxylase, biotin carboxyl carrier protein, and carboxyltransferase. Fragment-based screening revealed that amino-oxazole inhibits biotin carboxylase activity and also exhibits antibacterial activity against Gram-negative organisms. In this report, we redesigned previously identified lead inhibitors to …

Diagnosing The Third World: The “Map Doctor” And The Spatialized Discourses Of Disease And Development In The Cold War, Timothy Barney

Rhetoric and Communication Studies Faculty Publications

In the early 1950s, the American Geographical Society, in collaboration with the United States Armed Forces and international pharmaceutical corporations, instituted a Medical Geography program whose main initiative was the Atlas of Disease, a map series that documented the global spread of various afflictions such as polio, malaria, even starvation. The Atlas of Disease, through the stewardship of its director, Jacques May, a French-American physician trained in colonial Hanoi, evidenced the ways in which cartography was rhetorically appropriated in the Cold War as a powerful visual discourse of development and modernization, wherein both the data content of the maps and …

"Every Child In Our World Will Know His Name!": Malcolm Gladwell's Theories As An Explanation For The Cultural Phenomenon Of Harry Potter, Alicia Morgan

Masters Theses

When Harry Potter and the Sorcerer's Stone hit stores in the United States in 1998, children and adults alike went wild. Not only had Rowling's first book made huge waves in the UK, but Americans were talking about the struggling, single-parent who had penned a children's classic before the book even reached American stores. American audiences accepted Rowling's first novel with open arms, making it one of the first children's books to reach and occupy The New York Times best-seller list for so long. While certain marketing techniques and the rise of the Internet did contribute to Harry Potter's initial …

The Effect Of Fluvastatin On Mast Cell Function: Genotype Dependence, Elizabeth M. Kolawole

Theses and Dissertations

Fluvastatin, the HMG-CoA reductase inhibitor known for its role in the treatment of hypercholesterolemia and cardiovascular disease, has more recently been shown to play a role in the immune response. Given the critical role that mast cells play in allergy and inflammatory diseases such as asthma, which effects one third of America’s population, we assessed the effect of fluvastatin on mast cell and basophils function. We demonstrate that fluvastatin downregulated IgE-mediated cytokine production. Additionally, in vivo studies showed that fluvastatin suppressed IgE-mediated anaphylaxis. Interestingly, the effects of fluvastatin showed dependence on genetic background, as C57BL/6 mast cells were sensitive, while …

The Effect Of Lactic Acid On Mast Cell Function, Andrew J. Spence

Theses and Dissertations

This study shows for the first time the effect that L-(+)-lactic acid has on mast cell activation. Lactic acid is a byproduct of anaerobic glycolysis and is associated with inflammatory environments such as wounds, tumors and, asthma. In this study, pre-treatment with lactic acid altered cytokine production by bone marrow-derived mast cells (BMMC). Specifically, lactic acid enhanced cytokine secretion following IgE cross-linking, but decreased IL-33 mediated cytokine production. These effects were altered by genetic background, since C57BL/6 mast cells demonstrated the aforementioned result, but lactic acid had no effect on IgE-mediated cytokine production in 129/SvJ mast cells. The affected cytokines …

Undergraduate And Graduate Course Descriptions, 2014 Spring, Wright State University

Course Descriptions

Wright State University undergraduate and graduate course descriptions from Spring 2014.

Alcohol Use Disorders And Antiretroviral Therapy Among Prisoners With Hiv/Aids In Argentina, Michael Drake Alpert

Yale Medicine Thesis Digital Library

While Argentina has significantly improved access to HIV care and antiretroviral therapy (ART) for both the general population and prisoners, the prevalence of alcohol use disorders (AUDs) among HIV-infected prisoners and their relationship to accessing ART in Argentina is currently unknown. This study aims to characterize the substance abuse patterns of HIV-infected prisoners in Argentina and to assess the independent correlates of receipt of pre-incarceration ART.

An anonymous, cross-sectional survey of 100 HIV-infected federal prisoners was conducted in the Buenos Aires municipality from July-December 2010. AUDs were assessed using the AUDIT scale.

A majority (63 per cent) of participants met …

Longitudinal Characteristics Of Depression And Mood States Beginning In Primary Hiv Infection, Jessica Ashley Gold

Yale Medicine Thesis Digital Library

LONGITUDINAL CHARACTERIZATION OF DEPRESSION AND MOOD STATES BEGINNING IN PRIMARY HIV INFECTION. Jessica A. Gold, Marie Grill, Julia Peterson, Christopher Pilcher, Evelyn Lee, Frederick M. Hecht, Dietmar Fuchs, Constantin T. Yiannoutsos, Richard W. Price, Kevin Robertson, and Serena Spudich. Department of Neurology, Yale University, School of Medicine, New Haven, CT.

Though depression is known to frequently afflict those with chronic HIV, mood during the early course of HIV is not well characterized. In a prospective study we assessed mood during primary HIV infection (PHI, <1 year duration), its association with neuropsychological performance and markers of neurological disease, and its longitudinal course including effects of highly active antiretroviral therapy (HAART). The Beck Depression Inventory (BDI) and Profile of Mood States (POMS) subscales were longitudinally administered prior to and after HAART in PHI subjects. This evaluation of mood was done concurrently with blood, cerebrospinal fluid (CSF) and neuropsychological (total z and global deficit score, GDS) evaluation at each visit. Analysis employed Spearman's rho, logistic regression, and linear mixed models. 47.7% of the 65 men recruited at a median 3.5 months HIV duration met BDI criteria for clinical depression at baseline, classified as `mild' (n=11), `moderate' (n=11), or `severe' (n=9). Drug, alcohol, and depression history did not associate with BDI score. Proportional somatic-performance scores were worse than cognitive-affective scores (p=. 0045). Vigor subscore of POMS was reduced compared to norms and correlated with total z (r=0.33, p=0.013) and GDS (r=-0.32, p=0.016). BDI and POMS correlated with one another (r=0.85, p< .0001), but not with CSF or plasma HIV RNA, white blood cell (WBC) count, albumin ratio or neopterin. Improvement was not observed in BDI and POMS over 330 total follow-up visits, even after initiation of HAART. Depression was prevalent during PHI in our subjects, associated with abnormal somatic-performance and vigor scores. Neither neuropsychological performance nor disease biomarkers correlated with depressed mood. Mood indices did not improve over time in the presence of HAART.

Prevalence Of Tuberculosis Symptoms And Latent Tuberculosis Infection Among Prisoners In Northeastern Malaysia, Benjamin Margolis

Yale Medicine Thesis Digital Library

Background: Tuberculosis (TB) is a known public health threat to prison systems due to poor hygiene, crowded living conditions, poor health of inmates, and poor prison healthcare systems. The large population of HIV-infected people living in prisons is especially susceptible to increased TB transmission and higher mortality. In Northeastern Malaysia, there is no screening for active or latent TB infection (LTBI) in the prison system.

Aims and Hypothesis: This study serves to measure the prevalence and correlates of LTBI and active TB symptoms in HIV-infected and non-HIV-infected prisoners in Northeastern Malaysia. Given the host of factors that contribute to TB …

Safety And Efficacy Of Ceftaroline Fosamil In The Management Of Community-Acquired Bacterial Pneumonia, Heather F. Debellis, Kimberly L. Barefield

PCOM Scholarly Works

Ceftaroline fosamil is a new fifth-generation cephalosporin indicated for the treatment of community-acquired bacterial pneumonia (CABP). It possesses antimicrobial effects against both Gram-positive and Gram-negative bacteria, including methicillin-resistant Staphylococcus aureus (MRSA), but not against anaerobes. Organisms covered by this novel agent that are commonly associated with CABP are Streptococcus pneumoniae, Staphylococcus aureus, Haemophilus influenzae, Moraxella catarrhalis, and Klebsiella pneumoniae; however, ceftaroline fosamil lacks antimicrobial activity against Pseudomonas and Acinetobacter species. FOCUS 1 and FOCUS 2 clinical trials evaluated the use of ceftaroline fosamil in the treatment of CABP as compared to ceftriaxone. These non-inferiority trials provided evidence that ceftaroline fosamil …

Antibiotic Lock Therapy: Review Of Technique And Logistical Challenges, Julie Ann Justo, P Brandon Bookstaver

Faculty Publications

Antibiotic lock therapy (ALT) for the prevention and treatment of catheter-related bloodstream infections is a simple strategy in theory, yet its real-world application may be delayed or avoided due to technical questions and/or logistical challenges. This review focuses on these latter aspects of ALT, including preparation information for a variety of antibiotic lock solutions (ie, aminoglycosides, beta-lactams, fluoroquinolones, folate antagonists, glycopeptides, glycylcyclines, lipopeptides, oxazolidinones, polymyxins, and tetracyclines) and common clinical issues surrounding ALT administration. Detailed data regarding concentrations, additives, stability/compatibility, and dwell times are summarized. Logistical challenges such as lock preparation procedures, use of additives (eg, heparin, citrate, or ethylenediaminetetraacetic …

Specimen Collection: An Essential Tool, L. A. Rocha, A. Aleixo, G. Allen, F. Almeda, C. C. Baldwin, M. V.L. Barclay, J. M. Bates, A. M. Bauer, F. Benzoni, C. M. Berns, M. L. Berumen, D. C. Blackburn, S. Blum, F. Bolaños, R. C.K. Bowie, R. Britz, R. M. Brown, C. D. Cadena, K. Carpenter, L. M. Ceríaco, P. Chakrabarty, G. Chaves, J. H. Choat, K. D. Clements, B. B. Collette, A. Collins, J. Coyne, J. Cracraft, T. Daniel, M. R. De Carvalho, K. De Queiroz, F. Di Dario, R. Drewes

Faculty Publications

No abstract provided.