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2014

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Increased Effluent Dosage Effects On Septic System Absorption-Field Products Of Differing Architecture Types, Andrew Richard Gibbons Dec 2014

Increased Effluent Dosage Effects On Septic System Absorption-Field Products Of Differing Architecture Types, Andrew Richard Gibbons

Graduate Theses and Dissertations

Nationwide, approximately 20% of all homes use an on-site septic system as a form of household wastewater treatment. Since karst features are prevalent throughout the Ozark Highlands region of Northwest Arkansas, surface and groundwater resources are susceptible to contamination. Therefore, the objectives of this study were to evaluate the effects of soil condition (i.e., wet and dry) and absorption-field-product architecture type [i.e., chamber, gravel-less-pipe (GLP), polystyrene-aggregate (PSA), and pipe-and-aggregate (PA)] on in-product solution storage and biomat thickness in a profile-limited soil, and to identify the long-term acceptance rate (LTAR) of each product. During Phase I of this study (i.e., March …


Development And Characterization Of An Autologous Whole Cell Breast Cancer Vaccine, Samantha Leigh Kurtz Dec 2014

Development And Characterization Of An Autologous Whole Cell Breast Cancer Vaccine, Samantha Leigh Kurtz

Graduate Theses and Dissertations

Approximately 40,000 women will die from breast cancer in the United States in 2014. About 90% of these deaths will be due to metastases, rather than the primary tumor and majority of metastases are due to the recurrence and progression of non-metastatic disease. Current adjuvant treatments, such as chemotherapy and radiation, have severe side effects and may result in overtreatment and drug resistance.

Since greater than 90% of patients are diagnosed between stages I-III and have minimal residual disease after treatment, there is an opportunity to treat patients with an autologous breast cancer vaccine. Autologous vaccines under development have a …


Career Decision-Making Self-Efficacy: Examining The Effects Of Career Choice, Education, And Work Experience Of Entrepreneurs, Melissa W. Sisco Dec 2014

Career Decision-Making Self-Efficacy: Examining The Effects Of Career Choice, Education, And Work Experience Of Entrepreneurs, Melissa W. Sisco

Graduate Theses and Dissertations

Prior research on the relationship between self-efficacy and career decision making is inconclusive because of the lack of theoretical background and causal conclusions. More research is needed to investigate how the educational background, career choice, and work experience, of entrepreneurs, influences their career decision-making self-efficacy beliefs and how those beliefs effect their decision to become entrepreneurs. The purpose of this study was to investigate to what extent career choice, education, and work experience related to the level of career decision-making self-efficacy among participants in the Ventureprise business incubation program who are or are intending to become entrepreneurs. The education and …


Population Genetics, Distributions And Phenology Of Bombus Latreille, 1802 And Xylocopa Latreille, 1802 (Hymenoptera: Apidae), Amber Dawn Tripodi Dec 2014

Population Genetics, Distributions And Phenology Of Bombus Latreille, 1802 And Xylocopa Latreille, 1802 (Hymenoptera: Apidae), Amber Dawn Tripodi

Graduate Theses and Dissertations

This work addresses multiple knowledge gaps in bee ecology, population health and phylogeography in order to provide insights into the changing distributions of native bees. A comparison of Arkansas bumble bee records mirrors range-wide surveys, with records of stable species (Bombus bimaculatus Cresson, 1863 and B. impatiens Cresson, 1863) increasing three-fold, and records of the declining B. pensylvanicus (DeGeer, 1773) dropping to 60% of historical levels. However, nationally-recommended conservation-genetics tools did not mirror these results on a regional level. Stable and declining species had equivalent genetic diversity in samples from Arkansas and Tennessee (HS range: 0.46-0.63). Diploid males, …


Ethnic And Language Matching: Effects On Hispanics' Treatment Perceptions, Carlos Adrian Ojeda Cedeno Dec 2014

Ethnic And Language Matching: Effects On Hispanics' Treatment Perceptions, Carlos Adrian Ojeda Cedeno

Graduate Theses and Dissertations

Cultural adaptations to mental health treatment have been recommended to improve treatment outcomes in minorities, including Hispanics (Griner & Smith, 2006). One such adaptation includes matching the therapist to the client on culturally salient variables, such as spoken language or ethnic background. Yet, most investigations about the efficacy of matching have been correlational or have not examined language and ethnic match together (Cabassa, 2007). I investigated the effects of both ethnic and language matching on Hispanics' perceptions of psychological treatment. Participants were 100 Hispanic adults (36 men) randomly assigned to one of four conditions. In each condition, participants read a …


Social Media In The Church, Derick Ray Mckinney Dec 2014

Social Media In The Church, Derick Ray Mckinney

Graduate Theses and Dissertations

This thesis project examines the effectiveness of social media in the church. Sites like Facebook, Twitter, Instagram, and YouTube have the potential to shape the way the world communicates. Many churches have even begun utilizing social media in their ministry efforts. They publicize, pray, encourage, and promote events, all using social media. This paper argues that Bible believing churches can effectively use social media to communicate to their members by announcing upcoming events, sharing audience specific information, and knowing the best time to post on these sites. This project shows a social media strategy implemented into New Heights Church in …


Life Cycle Assessment Projection Of Photovoltaic Cells: A Case Study On Energy Demand Of Quantum Wire Based Photovoltaic Technology Research, Shilpi Mukherjee Dec 2014

Life Cycle Assessment Projection Of Photovoltaic Cells: A Case Study On Energy Demand Of Quantum Wire Based Photovoltaic Technology Research, Shilpi Mukherjee

Graduate Theses and Dissertations

With increasing clean-energy demand, photovoltaic (PV) technologies have gained attention as potential long-term alternative to fossil fuel energy. However, PV research and manufacture still utilize fossil fuel-powered grid electricity. With continuous enhancement of solar conversion efficiency, it is imperative to assess whether overall life cycle efficiency is also being enhanced. Many new-material PV technologies are still in their research phase, and life cycle analyses of these technologies have not yet been performed. For best results, grid dependency must be minimized for PV research, and this can be accomplished by an analytical instrument called Life Cycle Assessment (LCA).

LCA is the …


Geographic Assessment Of The Perception Of Nature Reserves And National Parks In Kuwait, Meshari S. Alenezi Dec 2014

Geographic Assessment Of The Perception Of Nature Reserves And National Parks In Kuwait, Meshari S. Alenezi

Graduate Theses and Dissertations

The Arabian Gulf countries have passed strict laws to preserve their environment. Kuwait has a strong history with preserving natural areas. The ecological value and richness of Kuwait's ecosystems have increased since the nature reserves were built. This research has evaluated the perceptions that Kuwaitis have of the design, creation, and development of nature reserves in Kuwait. It involved the use of survey instruments (questionnaires) and interviews with respondents of both urban and rural communities. Data from these surveys and interviews analyzed regarding perceptions of nature reserves' needs, sizes, functions, and future plans.

The results of examinations (surveys) demonstrate that …


Effects Of Grain Sorghum Muffin On Blood Glucose And Insulin Responses In Prediabetic Men, Xuan Gu Dec 2014

Effects Of Grain Sorghum Muffin On Blood Glucose And Insulin Responses In Prediabetic Men, Xuan Gu

Graduate Theses and Dissertations

Prediabetes is a sub-health condition in the development to type 2 diabetes, which has been long overlooked. Grain sorghum contains functional starch fractions, which have been widely reported for their potential on blood glucose control and diabetes prevention. A human study with prediabetic men was conducted to investigate the effects of sorghum starch on postprandial blood glucose and insulin levels. Grain sorghum and wheat (control) muffins containing 50 g total starch were consumed by 15 prediabetic male subjects on two mornings with a 1-week washout period. Plasma samples were collected on -15 (baseline), 0, 15, 30, 45, 60, 75, 90, …


Cross-Cultural Comparison In The Emotional Responses Elicited By Wine Odors, Aimee Hasenbeck Dec 2014

Cross-Cultural Comparison In The Emotional Responses Elicited By Wine Odors, Aimee Hasenbeck

Graduate Theses and Dissertations

To remain competitive in the wine market, wine companies must strive for a deeper understanding of the factors that influence consumers at an emotional level in order to sustain consumer satisfaction. Evaluating the wine odor-induced emotional response, in addition to hedonic response and descriptive analysis, may provide valuable information into the perspective of the consumer and potentially insight to repeat purchases. The objectives of this research were to measure the emotional responses elicited by odor attributes in wine samples and determine whether these emotional responses vary as function of cultural and experiential backgrounds, as well as sensory characteristics of wine …


Control Of Agrilus Ruficollis (Coleoptera: Buprestidae) With Insecticides And Identifying Visual Attractants For Use In A Monitoring Trap, Soo-Hoon Kim Dec 2014

Control Of Agrilus Ruficollis (Coleoptera: Buprestidae) With Insecticides And Identifying Visual Attractants For Use In A Monitoring Trap, Soo-Hoon Kim

Graduate Theses and Dissertations

The rednecked cane borer, Agrilus ruficollis (F.), is a pest of cultivated and wild blackberries in the Midwestern and Eastern parts of the United States. Feeding, mating, egg laying and development of A. ruficollis from larvae to adult only occurs on primocane, the first year vegetative growth stage of blackberries, and not on the second year fruiting stage called floricanes that die after fruiting. Damage from this pest is caused by the larva girdling the primocane and tunneling in the pith, causing the formation of a gall. Gall formation on the primocane increases the chance of winter injury and can …


The Role Of Time And Place In Understanding The Quality Of Life Among Homeless Persons, Gail O'Connor Dec 2014

The Role Of Time And Place In Understanding The Quality Of Life Among Homeless Persons, Gail O'Connor

Graduate Theses and Dissertations

This study examines the role that life chances and choices play in determining quality of life among homeless people. Given the prominent negative impact of homelessness, this paper specifically examines the impact of length of time homeless and location on adverse quality of life. Data from Birmingham, Alabama and Northwest Arkansas Point-in-Time Homeless Census' was utilized and combined to create a sample of 264 homeless individuals. Using a quantitative approach, the topic of adverse quality of life for the homeless is analyzed through a three-model OLS regression, using a life chances and choices framework, with the addition of experiential context--time …


Evaluation Of Low-Tannin Grain Sorghum In Broiler Chicken Diets, Samantha Aniecia Shelton Dec 2014

Evaluation Of Low-Tannin Grain Sorghum In Broiler Chicken Diets, Samantha Aniecia Shelton

Graduate Theses and Dissertations

Research trials were conducted to evaluate the effects of different levels of dietary grain sorghum on broiler live performance, carcass yield and shank (leg) and breast skin coloring. Iso-caloric diets were formulated where sorghum replaced corn at rates of 0% (control), 20, 40, 60, 80 and 100% for a total of 6 diets. For each of the two trials, 1500 Cobb 500 male broiler chicks were randomly allocated to 60 pens with 25 birds per pen (10 pens/diet) and grown to 46 days for the first research trial and 41 days for the second. There were no differences (P>.05) …


Recreational Risk Assessment Using Geospatial Analyses On Beaver Lake, Arkansas, Laura Ahrens Dec 2014

Recreational Risk Assessment Using Geospatial Analyses On Beaver Lake, Arkansas, Laura Ahrens

Graduate Theses and Dissertations

Accidents and deaths occur regularly on lakes and waterways due to natural phenomena as well as human error and recreation. People use lakes and waterways as sources of recreation, but do not always act responsibly on the water. During summer 2013 and winter 2014, over 100 surveys were administered in Beaver Lake, Arkansas to local and federal agencies regarding their knowledge and perception on accident locations and causes. These surveys were conducted in English and approved by the university after being tested on peers. The surveys included demographic data sex, income, education, as well as Likert-scaled responses. Research was also …


Central Administration Of Neuropeptide Y (Npy) And Vasotocin 4 Receptor (Vt4r/V1ar) Antagonists On Food Intake And A Stress Response In Chicks, Megan Ann Hancock Dec 2014

Central Administration Of Neuropeptide Y (Npy) And Vasotocin 4 Receptor (Vt4r/V1ar) Antagonists On Food Intake And A Stress Response In Chicks, Megan Ann Hancock

Graduate Theses and Dissertations

The objective of this study was to determine the central effect of the VT4R antagonists (SR-49059 and H-5350 (Manning compound)) on corticosterone (CORT) levels during stress and food intake. A 22-gauge stainless steel guide cannula was surgically implanted into the lateral ventricle of the birds. A preliminary study was done to test the antagonists and their role on food intake. Birds were injected with saline, NPY (4µg), NPY (4µg)+SR-49059 (250ng), or NPY (4µg)+Manning compound (250ng). Birds injected with saline had the lowest 1h food intake (17.7g1.6). Birds injected with NPY+saline had a significantly higher intake (27.1g 1.0), which was enhanced …


Acanthamoeba Spp. As Reservoirs For Transmission Of Norovirus, Tun-Yun Hsueh Dec 2014

Acanthamoeba Spp. As Reservoirs For Transmission Of Norovirus, Tun-Yun Hsueh

Graduate Theses and Dissertations

Human noroviruses (HuNoV) are the most common cause of foodborne disease outbreaks in the United States and the most common food commodities implicated in HuNoV outbreaks are leafy greens; however, the vehicle of transmission and point of contamination are often unknown. Here, we hypothesize that common free-living amoebae (FLA) ubiquitous in the environment may act as reservoirs of HuNoV and facilitate the transmission of these pathogens to fresh produce. The objective of this research was to first evaluate the interaction/association between HuNoV surrogates and Acanthamoeba by incubating them together and analyzing virus titer associated with amoeba through an 8 day …


A Wellness Profile Of Student-Veterans At 4-Year Higher Education Institutions: The Role Of Gender, Combat Tours, And Deployment, William Monroe Heath Dec 2014

A Wellness Profile Of Student-Veterans At 4-Year Higher Education Institutions: The Role Of Gender, Combat Tours, And Deployment, William Monroe Heath

Graduate Theses and Dissertations

Afghanistan and Iraq war student-veterans have increased in population on college campuses in the United States (U.S.) as a result of using the Post 9/11 GI Bill. Healthy Campus 2020 (American College Health Association, 2012) highlighted the importance of college student wellbeing, health promotion and disease prevention, while the U.S. Department of Veteran Affairs has called for an approach to services that is strength based (United States Department of Veteran Affairs, 2008). To date, however, wellness studies conducted on Iraq and Afghanistan student-veterans using an evidenced-based model of holistic wellness have not been conducted. This study was designed to address …


Genetic Health And Population Viability Of Reintroduced American Marten In Michigan, Tamara L. Hillman Dec 2014

Genetic Health And Population Viability Of Reintroduced American Marten In Michigan, Tamara L. Hillman

Masters Theses

American marten (Martes americana) were extirpated from Michigan’s Lower Peninsula (LP) in 1911, and subsequently from the Upper Peninsula (UP) in 1939 due to habitat loss and unregulated trapping. The species was later reintroduced in the UP in the mid-1950s, and to the LP in the mid-1980s. Previous research has determined the small founding sizes used in the LP reintroductions have resulted in losses of genetic diversity, while research in the UP has produced discordant results concerning the effects of the reintroduction methods on genetic health and population structure. Since past research of marten in the LP, no …


The Nutrient Intake Of Homeless Women Of Grand Rapids, Michigan, Emily D. Popma-Metsaars Dec 2014

The Nutrient Intake Of Homeless Women Of Grand Rapids, Michigan, Emily D. Popma-Metsaars

Masters Theses

Background Homeless individuals comprise about 1% of the American population with 1/3 of this particular population being women. And despite the potential for hunger, the homeless population has a similar prevalence of overweight/obese as other Americans. The Heartside neighborhood of Grand Rapids is a very low-income area of the city, inhabited by the poor and homeless. The Food Access in Michigan Project is studying the relationship between food insecurity and food environments in Michigan.

Objectives The purpose of this study was to determine the characteristics of the diet of homeless women in Heartside. This study examined the level of food …


Effects Of Fatty Acid Structure On Endothelial Cell Expression Of Connexin43, Albert E. Sowers Iii Dec 2014

Effects Of Fatty Acid Structure On Endothelial Cell Expression Of Connexin43, Albert E. Sowers Iii

Masters Theses

The epidemic of obesity-related metabolic and cardiovascular diseases is linked to elevated fatty acids (FA). Epidemiological data argue that trans FA contribute to the development and progression of these disease processes. Previous work suggests the similarity in structure between saturated and trans FA may stimulate inflammation through trans FA interactions with toll-like receptor 4 (TLR4). To evaluate the impact of trans FA on cardiovascular health, the response of endothelial cells to trans FA was evaluated. Specifically, responses to the 18-carbon trans FA elaidic acid (EA) were compared to the same length cis FA oleic acid (OA). Endothelial cells were exposed …


Knowledge, Practice, And Barriers Toward Cervical Cancer Screening In Elmina, Southern Ghana, Nancy Innocentia Ebu, Sylvia C. Mupepi, Mate Peter Siakwa, Carolyn M. Sampselle Dec 2014

Knowledge, Practice, And Barriers Toward Cervical Cancer Screening In Elmina, Southern Ghana, Nancy Innocentia Ebu, Sylvia C. Mupepi, Mate Peter Siakwa, Carolyn M. Sampselle

Peer Reviewed Articles

Aims: The aims of this study were: 1) to assess the level of knowledge of women about Pap smear tests, 2) to determine the practices of women regarding Pap smear tests, and 3) to determine the barriers to Pap smear tests in Elmina, Ghana.

Methods: A cross-sectional study was conducted with 392 randomly selected sexually active females aged 10–74 years using structured interview questions. The Institutional Review Board of the University of Cape Coast gave ethical approval for the study and informed consent was obtained from participants. Data were analyzed with SPSS software (v19.0) using frequencies, chi-square test, and exploratory …


Carpal Tunnel Syndrome: A Retrospective Analysis Of Conservative Treatments, Sarah E. Johnson, Britta L. Carlson, Maureen G. Meyers Dec 2014

Carpal Tunnel Syndrome: A Retrospective Analysis Of Conservative Treatments, Sarah E. Johnson, Britta L. Carlson, Maureen G. Meyers

Hand and Upper Extremity

Objective: The purpose of this study was to identify which conservative occupational therapy (OT) treatment(s) were most effective in reducing pain in patients diagnosed with carpal tunnel syndrome (CTS).

Design: A retrospective study of 222 de-identified treatment cases of patients seen from January 1st, 2004 to December 31st, 2013 were examined. Paired sample t-tests determined statistical significance between eight initial and final outcome measures. Binary logistic regressions determined statistical significance of clinically effective treatments that reduced pain at rest and pain with activity.

Setting: The 222 cases were from 31 outpatient rehabilitation clinics offering occupational therapy services.

Subjects: Participants were …


Young Leaders Of Character Program: A Model Of Character Education Program For Improving Life Effectiveness Skills And Civic Responsibility Of Adolescents, Bengü Ergüner-Tekinalp, Kris Crabtree-Groff Dec 2014

Young Leaders Of Character Program: A Model Of Character Education Program For Improving Life Effectiveness Skills And Civic Responsibility Of Adolescents, Bengü Ergüner-Tekinalp, Kris Crabtree-Groff

Journal of Youth Development

Studies that examine character development programs are scarce. This study examines the effect of a week-long character education program in a range of life skills and civic efficacy. Thirty adolescents participated in the training. A no-control, quasi-experimental design incorporated baseline measures and a six-month follow-up. A Life Effectiveness Questionnaire and Civic Efficacy Survey were administered and open-ended questions further explored how participants incorporated program outcomes into their daily lives. The t-test comparison of baseline and pretest measures yielded no significant differences, but t-test comparison of pre-post- test analysis elicited statistically significant positive results. Findings indicate the program can provide a …


Critical Literacy Development In Early Childhood Education, Blandina Francis Duwe Dec 2014

Critical Literacy Development In Early Childhood Education, Blandina Francis Duwe

Master of Education

Developing critical literacy using action research entails reading African stories to children and asking questions to direct children’s thinking. The idea of critical literacy development arises from the need to empower young children to be engaged in discussion about different issues that are directly or indirectly connected to their lives. This study employed the use of qualitative approach and action research design to explore how critical literacy could be developed in young children. Results found indicated that children can engage in exploring complex issues as long as they are exposed to activities that stimulate thinking to development of critical literacy. …


“Aho! All My Relations:” Native Identity And Its Relationship To Psychological Well-Being, Jamie Ramsey Dec 2014

“Aho! All My Relations:” Native Identity And Its Relationship To Psychological Well-Being, Jamie Ramsey

All-Inclusive List of Electronic Theses and Dissertations

This is the first known study to examine the relationship between Native identity and psychological well-being, which is defined by Ryff in positive psychology as existential strengths. It is also the first known study to investigate the relationship between Native identity and blood quantum through quantitative measures. Overall, 199 Natives from two American Indian Centers, three Indiana powwows, and online from Facebook participated by completing the Ryff’s Scales of Psychological Well-being, Phinney’s Multigroup Ethnic Identity Measure, and demographic information. The main hypothesis was to explore whether any of the psychological well-being subscales (positive relations, autonomy, mastery of environment, self-acceptance, personal …


Alcohol And Drug Use Amongst College Students In Relation To Sexual Abuse, Christyn Hollingsworth Dec 2014

Alcohol And Drug Use Amongst College Students In Relation To Sexual Abuse, Christyn Hollingsworth

Honors College Theses

The impact of child sexual abuse (CSA) and traumatic sexual experiences has been the subject of multiple studies in which harm was found. Among these harms are emotional distress, psychological issues, and substance abuse. This research analyzed and compared the responses to questions pertaining to substance use and attitudes regarding sexual interactions between college aged students with and without a history of sexual abuse. The participant’s answers were run through an ANOVA to determine statistical variance. Based on prior research, it was expected that victims consume alcohol and use drugs more frequently than non-victims in significant amounts. The findings in …


Culture In Solution-Focused Consultation: An Intercultural Approach*, Gale Miller Dec 2014

Culture In Solution-Focused Consultation: An Intercultural Approach*, Gale Miller

Journal of Solution Focused Practices

I develop an intercultural approach to Solution-Focused consultation in this pa­ per. The approach represents an anthropological-sociological interpretation and is written by an interested outsider to the Solution-Focused world. The analysis stresses how multicultural concerns permeate Solution-Focused consultations as practitioners and clients draw from other cultures in which they participate. Prac­titioner-client interactions are treated as negotiations that turn on participants' intercultural competencies, that is, their ability to navigate between their own cultural orientations and those of others involved in consultations. An explanation of how my intercultural perspective extends cultural themes in the Solution-Fo­cused literature is discussed and an illustration of …


Not All Who Wander Are Lost: Wandering And Wondering In The Solution-Focused World. An Interview With Frank N. Thomas, Frank Thomas, Michael Durrant Dec 2014

Not All Who Wander Are Lost: Wandering And Wondering In The Solution-Focused World. An Interview With Frank N. Thomas, Frank Thomas, Michael Durrant

Journal of Solution Focused Practices

Dr. Frank Thomas is Professor of Counseling and Counselor Education at Texas Christian University in Fort Worth (Texas, US). He is also official Archivist for the Solution-Focused Brief Therapy Association. Frank and i have been friends and colleagues for nearly 25 years, co-presented at international conferences and writ­ ten book chapters and professional articles together. I endorsed his latest book, Solution-Focused Supervision: A resource-oriented approach to developing clinical expertise Springer), describing it as "THE most significant Solution-Focused book of the past 10 years." Frank is also an Associate Editor of JSFBT.


Katie Westermayer Honors Portfolio, Katie Lynn Westermayer Dec 2014

Katie Westermayer Honors Portfolio, Katie Lynn Westermayer

Honors Capstone Portfolios

Katie Westermayer's honors portfolio captured in December 2014.


Site-Specific Mutation Of The Sensor Kinase Gras In Staphylococcus Aureus Alters The Adaptive Response To Distinct Cationic Antimicrobial Peptides, Ambrose L. Cheung, Arnold S. Bayer, Michael R. Yeaman, Yan Q. Xiong, Alan J. Waring, Guido Memmi, Niles Donegan Dec 2014

Site-Specific Mutation Of The Sensor Kinase Gras In Staphylococcus Aureus Alters The Adaptive Response To Distinct Cationic Antimicrobial Peptides, Ambrose L. Cheung, Arnold S. Bayer, Michael R. Yeaman, Yan Q. Xiong, Alan J. Waring, Guido Memmi, Niles Donegan

Dartmouth Scholarship

The Staphylococcus aureus two-component regulatory system, GraRS, is involved in resistance to killing by distinct host defense cationic antimicrobial peptides (HD-CAPs). It is believed to regulate downstream target genes such as mprF and dltABCD to modify the S. aureus surface charge. However, the detailed mechanism(s) by which the histidine kinase, GraS, senses specific HD-CAPs is not well defined. Here, we studied a well-characterized clinical methicillin-resistant S. aureus (MRSA) strain (MW2), its isogenic graS deletion mutant (ΔgraS strain), a nonameric extracellular loop mutant (ΔEL strain), and four residue-specific ΔEL mutants (D37A, P39A, P39S, and D35G D37G D41G strains). The ΔgraS and …