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Phosphatase-Inert Glucosamine 6‑Phosphate Mimics Serve As Actuators Of The Glms Riboswitch, Xiang Fei, Thomas Holmes, Julianna Diddle, Lauren Hintz, Dan Delaney, Alex Stock, Danielle Renner, Molly Mcdevitt, David B. Berkowitz, Juliane K. Soukup

David Berkowitz Publications

The glmS riboswitch is unique among generegulating riboswitches and catalytic RNAs. This is because its own metabolite, glucosamine-6-phosphate (GlcN6P), binds to the riboswitch and catalytically participates in the RNA selfcleavage reaction, thereby providing a novel negative feedback mechanism. Given that a number of pathogens harbor the glmS riboswitch, artificial actuators of this potential RNA target are of great interest. Structural/kinetic studies point to the 2- amino and 6-phosphate ester functionalities in GlcN6P as being crucial for this actuation. As a first step toward developing artificial actuators, we have synthesized a series of nine GlcN6P analogs bearing phosphatase-inert surrogates in place …

Generalized Weibull And Inverse Weibull Distributions With Applications, Valeriia Sherina

College of Graduate Studies: Theses & Dissertations

In this thesis, new classes of Weibull and inverse Weibull distributions including the generalized new modified Weibull (GNMW), gamma-generalized inverse Weibull (GGIW), the weighted proportional inverse Weibull (WPIW) and inverse new modified Weibull (INMW) distributions are introduced. The GNMW contains several sub-models including the new modified Weibull (NMW), generalized modified Weibull (GMW), modified Weibull (MW), Weibull (W) and exponential (E) distributions, just to mention a few. The class of WPIW distributions contains several models such as: length-biased, hazard and reverse hazard proportional inverse Weibull, proportional inverse Weibull, inverse Weibull, inverse exponential, inverse Rayleigh, and Frechet distributions as special cases. Included …

Generalized Classes Of Distributions With Applications To Income And Lifetime Data, Shujiao Huang

College of Graduate Studies: Theses & Dissertations

In this thesis, new classes of distributions namely: exponentiated Kumaraswamy-Dagum (EKD), Log-exponentiated Kumaraswamy-Dagum (Log-EKD), McDonald Log-logistic (McLLog) and Gamma-Dagum (GD) distributions are presented. A thorough and comprehensive investigation of these classes of distributions is conducted. Mathematical properties of these classes of distributions including series expansion, hazard and reverse hazard functions, moments, generating functions, mean and median deviations, Bonferroni and Lorenz curves, distribution of order statistics, moments of order statistics and entropies are presented. Estimation of parameters of these distributions via maximum likelihood technique, Fisher information and asymptotic confidence intervals are given. Maximum likelihood estimation of the parameters of the exponentiated …

Isolation And Characterization Of Multipotent Lung Stem Cells From P53 Mutant Mice Models, Venkat Sundar Gadepalli

Theses and Dissertations

Recent advances in understanding lung biology have shown evidence for the existence of resident lung stem cells. Independent studies in identifying and characterizing these somatic lung stem cells have shown the potential role of these cells in lung repair and regeneration. Understanding the functional characteristics of these tissue resident stem/progenitor cells has gained much importance with increasing evidence of cancer stem cells, cells in a tumor tissue with stem cell characteristics. Lung cancer is most commonly characterized by loss of p53 function which results in uncontrolled cell divisions. Incidence of p53 point mutations is highest in lung cancer, with a …

Reproductive Biology Study Of Dynamics Of Female Sexual Hormones: A 12-Month Exposure To Lead Acetate Rat Model, Eugenia Dumitrescu, Romeo Teodor Cristina, Florin Muselin

Turkish Journal of Biology

In human and animal organisms, lead can cause reproductive problems beginning with pregnant females. The reproductive axis is particularly sensitive to lead, its influence resulting in a delayed sexual maturity due to biosynthesis suppression of the sexual steroids. An animal model study was carried out on 28 white Wistar adult female rats, divided into 3 experimental (E) groups that were exposed for 12 months to lead acetate in drinking water as follows: 50 ppb Pb (E_1), 100 ppb Pb (E_2), and 150 ppb Pb (E_3), with a control group (M) that received unleaded tap water. Levels of FSH, LH, estradiol, …

Cover And Contents

Turkish Journal of Biology

No abstract provided.

Characterization Of A Putative Tonb Deficient Porphyromonas Gingivalis Mutant, Soheil Rostami

Theses and Dissertations

Porphorymonas gingivalis is one of the major bacterial pathogens responsible for the initiation and progression of periodontal disease. The bacterium requires hemin uptake for its growth and has developed sophisticated mechanisms to extract hemin from hemin containing proteins in the oral cavity. Hemin first binds to receptors on the surface of P. gingivalis and is then taken up in an energy dependent manner. TonB is an inner membrane bound protein that spans the periplasm and is believed to be involved in the passage of hemin through the double membrane of P. gingivalis. However, the TonB protein in P. gingivalis is …

A Pollination Network Of Cornus Florida, James H. Lee

Theses and Dissertations

From the agent-based, correlated random walk model presented, we observe the effects of varying the parameter values of maximum insect turning area, ��_max, density of trees, ω, maximum pollen carryover, ��_max, and probability of fertilization, P_��, on the distribution of pollen within a population of Cornus florida (flowering dogwood). We see that varying ��_max and ��_max changes the dispersal distance of pollen, which greatly affects many measures of connectivity. The clustering coefficient of fathers is maximized when ��_max is between 60° and 90°. Varying ω does not have a major …

Energy Tradeoffs Between Food Assimilation, Growth, Metabolism And Maintenance, Lihong Jiao

Masters Theses

"The effect of metabolic rate (MR) on organisms' health maintenance is a long-standing puzzle and empirical data on this issue is contradictory. A theoretical model was developed for understanding animal's energy budget under the food condition of Ad libitum (AL) and food restriction. This model offers a framework for understanding the role of MR and health maintenance mechanism from the perspective of energy tradeoff between food assimilation, growth, metabolism and maintenance. Hornworm (Manduca sexta larva) has been selected as an model to test the energetic tradeoff under different food supply and ambient temperatures. The changes in energy budget can …

Molecular Profiling In Cervical Carcinogenesis And Progression, Kathryn Elizabeth Royse

All ETDs from UAB

Coexisting lesions can reduce genomic heterogeneity in precancer progression analysis by reducing variation and bias that can mask gene effects. Our goal was to use novel methodologies to depict the neoplastic stage effect (Normal v. LSIL v. HSIL) in cervical precancer. We analyzed the neoplastic stage effect via in silico and in vivo methodologies. For in silico analyses, we calculated differential expression (DE) estimates from a systematic review of DNA methylation and gene expression literature. Significant genes (FC≤2.0 or p-value≥0.05) were grouped by histology for pathway analysis. For in vivo analyses, we performed RNA-seq on microdissected FFPE coexisting cervical tissue …

Effect Of Dietary Β-Glucan Supplementation On Growth, Intestinal Functionality And Disease Progression In Selected Cyprinids (Cyprinus Carpio And Danio Rerio), Holger Kühlwein

Other Faculty of Science and Engineering Theses

Three experiments were conducted to investigate the effects of dietary supplementation of a ß-(1,3)(1,6)-D-glucan derived from the yeast Saccharomyces cerevisiae, on growth performance parameters and intestinal functionality in mirror carp (Cyprinus carpio L.) and on progression of a chronic, pre-existing mycobacteriosis in zebrafish (Danio rerio). The 1st experiment revealed that mirror carp fed diets containing 1% and 2% ß-glucan showed significant improvements in growth performance compared to fish fed both the control and the 0.1% ß-glucan containing diet. Equally, fish fed diets supplemented with 1% and 2% ß-glucan displayed significantly higher infiltration of leukocytes into the epithelial layer of in …

Understanding The Impact Of Adolescent Attachment On Academic Success, Cristina Cortesi

Dissertations

In recent years, studies are citing an increase in adolescent mental health diagnoses and symptomotology related to AD/HD, Bipolar Disorder, Depression, Anxiety, and Conduct Disorders. While the associated behaviors may be the result of several variables, recent neurobiological studies combined with Attachment Theory have pointed to a possible link to attachment issues. Because of the developmental stage of adolescents, these behaviors have been observed in both home and school environments. Schools, in general in the United States, have been experiencing significant struggles in terms of lack of adequate educational success which prompted the implementation of legislation commonly known as No …

Systematics And Species Delimitation In New Guinea Skink Species Complexes (Squamata: Scincidae), Eric Nikolaus Rittmeyer

LSU Doctoral Dissertations

Though among the most controversial topics in systematic and evolutionary biology, species are a fundamental unit in biology, and are utilized by and critical to a wide variety of studies in the life sciences. Despite this importance, little work has focused on developing and examining objective methods for species delimitation until recently. Further, New Guinea and the surrounding regions are among the most diverse and geologically complex regions globally, yet the region remains poorly explored biologically, and little work has examined the evolutionary history of the fauna in the region. To investigate the influence of factors such as sampling intensity, …

Design, Synthesis, And Antibacterial Properties Of Dual-Ligand Inhibitors Of Acetyl-Coa Carboxylase And Mechanism Of Action For The Antibacterial Agent Moiramide B, Molly Anne Silvers

LSU Doctoral Dissertations

An urgent demand for the development of new antibiotics has been created by the increase in clinical cases involving drug-resistant pathogenic bacteria. A novel target for antibacterial development is acetyl-CoA carboxylase, a multifunctional enzyme that catalyzes the first committed step in fatty acid synthesis. The bacterial form of the enzyme consists of three proteins: biotin carboxylase, biotin carboxyl carrier protein, and carboxyltransferase. The research presented in this dissertation describes dual-ligand inhibitors incorporating selective inhibitors against biotin carboxylase and carboxyltransferase that were synthesized by covalently attaching them with saturated hydrocarbon linkers containing 7 and 15 carbon atoms. The ability to inhibit …

Environmental Siting Suitability Analysis For Commercial Scale Ocean Renewable Energy: A Southeast Florida Case Study, Amanda Mulcan

Electronic Theses and Dissertations 2010 - 2019

This thesis aims to facilitate the siting and implementation of Florida Atlantic University Southeast National Marine Renewable Energy Center (FAU SNMREC) ocean current energy (OCE) projects offshore southeastern Florida through the analysis of benthic anchoring conditions. Specifically, a suitability analysis considering all presently available biologic and geologic datasets within the legal framework of OCE policy and regulation was done. OCE related literature sources were consulted to assign suitability levels to each dataset, ArcGIS interpolations generated seafloor substrate maps, and existing submarine cable pathways were considered for OCE power cables. The finalized suitability map highlights the eastern study area as most …

The Jumping Plant-Lice (Hemiptera: Psylloidea) Of Turkey: A Checklist And New Records, Jowita Drohojowska, Daniel Burckhardt

Turkish Journal of Zoology

A checklist is provided for the Turkish Psylloidea comprising 99 species, of which 1 is doubtful (Livia crefeldensis) and 6 are recorded for the first time from Turkey (Rhodochlanis bicolor, Euphyllura pakistanica, Camarotoscena lauta, Syntomoza unicolor, Baeopelma colorata, and Spanioneura persica). A key is provided for the 3 Spanioneura species associated with Prunus dulcis. Spanioneura pechai (Klimaszewski and Lodos) stat. rev. is removed from synonymy with S. turkiana.

Genetic Predisposition To Increased Blood Cholesterol And Triglyceride Lipid Levels And Risk Of Alzheimer Disease: A Mendelian Randomization Analysis, Alison Goate, Carlos Cruchaga, Petra Nowotny, John C. Morris, Kevin Mayo, Et Al

Open Access Publications

No abstract provided.

Synthesis Of Hybrid Inositol Glycan Analogues, Sudi Sabet

Master's Theses

A 2010 study by Centers for Disease Control and Prevention has projected that one of three US adults could have diabetes by the year 2050. More than 90% of the diagnosed cases are Type II diabetes, a condition characterized by insulin resistance. In search of new antidiabetic drugs, a class of natural, phosphorylated, inositol-containing pseudosaccharides called inositol glycans (IGs) has been studied for years. These compounds have insulin-mimetic activity in insulin-sensitive cells and can stimulate processes such as lipogenesis and glucose transport. Due to the heterogeneity of IGs, it is very difficult to isolate them from their natural sources, and …

Using Stable Isotope Analysis To Study Altitudinal And Latitudinal Bat Migration, Lily Arias

All-Inclusive List of Electronic Theses and Dissertations

The general lack of knowledge on basic aspects of the biology of temperate and tropical bats, their low reproductive rates, and threats such as white-nose syndrome, wind farms, and habitat loss, make them very susceptible to population declines. My research uses an innovative technique, the analysis of stable isotopes, to study the ecology of bat migration with the main goals of contributing significantly to the understanding of bat biology and assessing the conservation status and susceptibility of bats. In the first chapter, I measured the content of hydrogen isotopes in fur samples of migratory bat species killed at a wind …

Novel Compounds As Potential Alzheimer's Disease Therapeutics And Inhibitors Of The Nlrp3 Inflammasome, Jeremy E. Chojnacki

Theses and Dissertations

Alzheimer’s disease is a devastating neurodegenerative disorder and the leading cause of dementia. The disease manifests via several pathologies including neuroinflammation, oxidative stress, metal ion dyshomeostasis, and cell death. To address the multifaceted nature of this disorder, the design of several diverse compounds, targeting many pathological effects, was generated. First, a series of compounds based on curcumin and diosgenin were synthesized following the bivalent design strategy. Two compounds were discovered to have neuroprotective ability, anti-oxidative function, and anti-Aß oligomerization (AßO) properties. A second set of molecules was also designed, wherein a hybrid compound strategy was utilized. Three hybrids were to …

Light Mediated Drug Delivery Using Photocaged Molecules And Photoswitchable Peptides, Deboleena Mitra

Theses and Dissertations

There are many different types of targeted therapy for cancer treatment. The method of light mediated targeted therapy that we have developed uses photocaged molecules and photoswitchable peptides.

In photocaging, a biologically active molecule is made inactive by the attachment of a photocleavable blocking group. On exposure to UV radiation the photocleavable entity is removed and the biologically active molecule is released. Using this concept we have designed a prodrug that consists of a cell impermeable hydrophilic molecule attached to a photocaged doxorubicin. Upon irradiation with UV light the photosensitive group is removed and cytotoxic doxorubicin is released at the …

Enhancement Of Gulf Killifish, Fundulus Grandis, Fitness And Reproduction, Joshua Thomas Patterson

LSU Doctoral Dissertations

Gulf killifish, Fundulus grandis, shows promise for commercial development as a marine baitfish species. Significant markets for F. grandis already exist throughout the Gulf Coast, with the vast majority of supply coming from wild-harvests, which can be hampered by seasonal availability and inconsistent fish health and size. The genus Fundulus also represents an important group of model vertebrates for biological study. A recent review paper described this genus as the premier teleost model for environmental biology. New information generated as a result of the work contained in this dissertation may be more broadly applicable to sister species of F. grandis, …

Biomarkers Of Alzheimer's Disease Risk In Peripheral Tissues; Focus On Buccal Cells, Maxime François, Wayne Leifert, Ralph Martins, Philip Thomas, Michael Fenech

Research outputs 2014 to 2021

Alzheimer's disease (AD) is a progressive degenerative disorder of the brain and is the most common form of dementia. To-date no simple, inexpensive and minimally invasive procedure is available to confirm with certainty the early diagnosis of AD prior to the manifestations of symptoms characteristic of the disease. Therefore, if population screening of individuals is to be performed, more suitable, easily accessible tissues would need to be used for a diagnostic test that would identify those who exhibit cellular pathology indicative of mild cognitive impairment (MCI) and AD risk so that they can be prioritized for primary prevention. This need …

Investigating The Role Of Mechanical Properties In Biological Systems, Nicole Shamitko-Klingensmith

Graduate Theses, Dissertations, and Problem Reports (ETD)

There is considerable interest in measuring, with nanoscale spatial resolution, the physical and material properties of biological membranes and whole cells because of their role in the physiology of living systems. The atomic force microscope (AFM) has proven to be particularly well-suited for biological studies because samples can be maintained in near physiological conditions to preserve sample integrity (such as in a buffer solution at a physiologically relevant pH or temperature), and the imaging process is relatively nondestructive. In this work, the mechanical properties of supported lipid bilayers and neurons were examined using AFM-based techniques. Specifically, tapping mode AFM-based techniques …

Association And Bioavailability Of 17Β-Estradiol With Soil And Manure Aqueous Dissolved And Colloidal Fractions, Katrin Bella Chambers

Soil Science

Steroidal estrogens in the environment exert toxicological effects at very low concentrations. Furthermore, dissolved and colloidal fractions of soil and manure play an important role in the environmental fate and transport of steroidal estrogens. One objective of this study was to quantify the association of the natural estrogen, 17β-estradiol (E2), with the dissolved fraction and colloidal fraction isolated from liquid swine manure (LSM), soil, and soil+LSM mixtures. The second objective of this study was to evaluate whether the E2 associated with the dissolved fraction/colloidal fraction, dissolved fraction and colloidal fraction of the various media could induce an estrogenic response. Estrogenicity …

Characterization Of A Novel Cryptosporidium Genotype In Red-Winged Black Birds, Jeba Rose Jennifer Jesudoss Chelladurai

Microbiological Sciences

Cryptosporidium species cause cryptosporidiosis, characterized by acute gastroenteritis in humans and animals worldwide. Knowledge of the diversity of Cryptosporidium among mammals and birds is incomplete, especially in North American passerines. In this first molecular study of Cryptosporidium in a North American passerine, C. parvum and a novel genotype, called the red-winged black bird genotype were isolated. Genetic characteristics and phylogenetic analyses of the red-winged black bird genotype were described at the 18S rRNA, actin and HSP70 loci, and it was distinct from previously described species and genotypes. The novelty of this genotype was also supported by propagation studies in chickens, …

Checklist Of Marine Tetrapods (Reptiles, Seabirds, And Mammals) Of Turkey, Harun Güçlüsoy, Emi̇ne Sühendan Karauz, Cem Orkun Kiraç, Murat Bi̇lecenoğlu

Turkish Journal of Zoology

The occurrence of a total of 61 marine tetrapod species is presented in this paper, including 3 sea turtles, 43 sea birds, and 15 marine mammals. Distribution of each reported species along the Black Sea, Sea of Marmara, Aegean, and Levantine coasts of Turkey is mentioned, associated with key references.

A Comparative Study Of The Diurnal Behaviour Of The Northern Shoveller (Anas Clypeata) During The Wintering Season At Garaet Hadj-Tahar (North-East Algeria) And Garaet Timerganine (Algerian Highlands), Sophia Metallaoui, Mohamed Cherif Maazi, Menouar Saheb, Moussa Houhamdi, Christophe Barbraud

Turkish Journal of Zoology

The rhythms of the Northern Shoveller's (Anas clypeata Linnaeus, 1758) activities during its wintering period in 2 wetlands, Garaet Hadj-Tahar (North-East Algeria) and Garaet Timerganine (Oum El-Bouaghi, Algerian highlands), were studied during 2 wintering seasons in Garaet Hadj-Tahar from November 2007 to March 2008 and November 2008 to March 2009, and in the Timerganine wetland from November 2007 to March 2008. They were linked to certain spatiotemporal variables, i.e. daytime activities and 2 different wetlands. During the wintering season, the main activity at Garaet Hadj-Tahar was sleeping (70%), followed by swimming (12%). By contrast, feeding was the dominant activity at …

Structure And Function Of Human Dnaj Homologue Subfamily A Member 1 (Dnaja1) And Its Relationship To Pancreatic Cancer, Jaime L. Stark, Kamiya Mehla, Nina V. Chaika, Thomas B. Acton, Rong Xiao, Pankaj K. Singh, Gaetano T. Montelione, Robert Powers

Robert Powers Publications

Pancreatic cancer has a dismal 5 year survival rate of 5.5% that has not been improved over the past 25 years despite an enormous amount of effort. Thus, there is an urgent need to identify truly novel yet druggable protein targets for drug discovery. The human protein DnaJ homologue subfamily A member 1 (DNAJA1) was previously shown to be downregulated 5- fold in pancreatic cancer cells and has been targeted as a biomarker for pancreatic cancer, but little is known about the specific biological function for DNAJA1 or the other members of the DnaJ family encoded in the human genome. …

A Novel Assembly Algorithm That Optimizes For Rna-Seq Data, Yi Yang

Theses and Dissertations

The advent of next-generation sequencing (NGS) technology has shown unprecedented promise for accurately identifying and quantifying genomic variants for living organisms. For species whose genome sequences are unknown, the first step of RNA sequencing data analysis is to assemble all short reads. The de Bruijn graph-based algorithms, such as Oases, are usually used for short reads assembly to resolve the issue of computational complexity. However, de Bruijn graph-based assemblers normally generate high error rates when assembling RNA-Seq data. We have developed a novel assembly algorithm that can be used jointly with any other assembly methods for RNA-Seq short reads. The …