Digital Commons Network^™

Hepatitis E Antibodies In Laboratory Rabbits From 2 Us Vendors, Leslie Birke, Stephania A. Cormier, Dahui You, Rhett W. Stout, Christian Clement, Merlin Johnson, Hilary Thompson

Faculty Publications

We tested laboratory rabbits from 2 US vendors for antibodies against hepatitis E virus (HEV); Seroprevalences were 40% and 50%. Retrospective analysis of an ocular herpes simplex 1 experiment demonstrated that HEV seropositivity had no effect on experiment outcome. HEV probably is widespread in research rabbits, but effects on research remain unknown.

Causes Of Annual Reproductive Variation And Anthropogenic Disturbance In Harlequin Ducks Breeding In Glacier National Park, Montana, Warren Kevin Hansen

Graduate Student Theses, Dissertations, & Professional Papers

Annual reproductive variation is the central focus of many ecological studies. Variation in reproductive success is an important vital rate to study because it can lead to inferences about population health, extinction risk, human disturbance and habitat quality. The identification of the causes of reproductive variability can help guide conservation and management efforts of a species. In Glacier National Park, Montana I studied causes of annual reproductive variation and behavioral responses to human disturbance in a breeding population of harlequin ducks (Histrionicus histrionicus). Harlequins are rare sea ducks with a Holarctic distribution and winter along rocky coast lines of North …

Identification Of Genomic Factors Using Family-Based Association Studies, Libo Wang

Open Access Dissertations

Genome-wide association studies become increasingly popular and important for detecting genetic associations of complex traits. However, it is well known that spurious associations could arise from statistical analysis without proper consideration of genetic relatedness of samples. Many methods have been proposed to guard against these spurious associations. Here we focus on multi-locus association studies of quantitative traits and the case-control status, and propose algorithms that take into consideration of genetic related samples to address possible confounding issues. As supervised dimension reduction methods, these algorithms performs well to conduct association studies with a large number of biomarkers but a relative small …

Monitoring Ligand-Induced Nucleic Acid Conformational Changes Using Ion Mobility Spectrometry-Mass Spectrometry, Bill Kenneth Redick

Legacy Theses & Dissertations (2009 - 2024)

Three-dimensional structures of biopolymers frequently dictate the biological role those molecules play. As such, investigation into structure of nucleic acids can provide important information pertaining to how those nucleic acids work. Many nucleic acid species, especially single-stranded RNA, fold into unique structures that allow them to function properly. Metals, and other cationic species, are often bound to the nucleic acid to make folding into the proper structure more favorable by neutralizing the negative charge on the nucleic acid imparted by the phosphate group. This investigation explores tertiary structure of nucleic acids that have been folded in the presence of ligands …

Inquiro 8 (End Matter), Inquiro Staff

Inquiro (undergraduate research journal)

pp. 65-70

Abolishing Exclusive Jurisdiction In The Federal Circuit: A Response To Judge Wood, Rochelle C. Dreyfuss

Faculty Articles

Part of a symposium of responses to Chief Judge Wood’s suggestion for giving regional circuits a share of the Federal Circuit’s authority over patent law, this article argues that now that a degree of nationwide uniformity in patent law has been achieved, it would be a pity to disrupt it. While Chief Judge Wood is right that the law would improve with percolation, a change in the composition of the court, new procedures for challenging patents in the Patent and Trademark Office, a District Court pilot program, and satellite patent offices will bring to the debate new voices, different kinds …

Genetic And Biochemical Studies Of Human Apobec3h Enzyme, Thisari Sachithra Aluthgama Guruge

Wayne State University Theses

The AID/APOBEC enzymes are DNA/RNA cytosine deaminases with important functions in innate and adaptive immunity. APOBEC3 enzymes play a crucial role in restricting the replication of exogenous retroviruses such HIV-1 and endogenous retrotransposition events. In particular, APOBEC3 enzymes have evolved in humans by gene duplication to compose seven members. APOBEC3H is highly diverse in its allelic sequence and the distribution is population-specific. It has evolved under strong positive selection over millions of years. To study the catalytic mechanism, structure, function and the underlying cause of its high diversity, a high resolution crystal or NMR structure is required are not yet …

Biosensors And Nanobiosensors: Design And Applications, Ahmed Touhami

Physics & Astronomy Faculty Publications

The goal of this chapter is to cover the full scope of biosensors. It offers a survey of the principles, design, operation, and biomedical applications of the most popular types of biosensing devices in use today. By discussing recent research and future trends based on many excellent books and reviews, it is hoped to give the readers a comprehensive view on this fast growing field.

Combining Motion Analysis And Microfluidics--A Novel Approach For Detecting Whole-Animal Responses To Test Substances., Tabitha S. Rudin-Bitterli, Oliver Tills, John I. Spicer, Phil F. Culverhouse, Eric M. Wielhouwer, Michael K. Richardson, Simon D. Rundle

School of Biological and Marine Sciences

Small, early life stages, such as zebrafish embryos are increasingly used to assess the biological effects of chemical compounds in vivo. However, behavioural screens of such organisms are challenging in terms of both data collection (culture techniques, drug delivery and imaging) and data evaluation (very large data sets), restricting the use of high throughput systems compared to in vitro assays. Here, we combine the use of a microfluidic flow-through culture system, or BioWell plate, with a novel motion analysis technique, (sparse optic flow - SOF) followed by spectral analysis (discrete Fourier transformation - DFT), as a first step towards automating …

Synthesis And Study Of Chemo-Hydrothermally Derived Water-Soluble Chitosan And Chiosan-Metal Oxide Composites, Srijita Basumallick

Electronic Theses and Dissertations

Chitosan (CS) is a man-made sugar based biopolymer derived from chitin, the second most abundant natural polymer after cellulose. Chitin is sourced from crustacean species such as shrimps and crabs. The chemical structure of chitin contains N-Acetyl D-glucosamine monomer units which forms CS upon deacetylation. In CS, ?-(1-4) linked D-glucosamine units are randomly distributed. Approximately 75% - 80% sugar units contains primary amine groups in commercially available low molecular weight CS. Biodegradability, low toxicity, mucoadhesive and transfecting properties of CS polymer are attractive for applications as oral and nasal drug delivery systems. Chitosan polymer is water insoluble at neutral pH. …

Kin 320.00: Physiology Of Exercise, Charles L. Dumke

University of Montana Course Syllabi

No abstract provided.

Expression And Clinical Implications Of P53, P63, And P73 Protein In Malignant Tumor Of The Parotid Gland, Mei Yong, Ling Yang, Qimuge Suyila, Wenyan Han, Hongwei Yuan, Cheng Zhao, Xiulan Su

Turkish Journal of Medical Sciences

To investigate the expression of P53, P63, and P73 proteins in malignant parotid gland tumors and adjacent nonneoplastic tissues and the association between the 3 proteins and their clinical characteristics. Materials and methods: A total of 40 pairs of paraffin-embedded malignant parotid gland tumors and adjacent nonneoplastic tissues were collected. We detected P53, P63, and P73 protein expression by immunohistochemistry. Statistical analysis was performed by using the chi-square test. Results: P53, P63, and P73 protein expression in malignant parotid gland tumors was higher than their expression in adjacent nonneoplastic tissue (P = 0.030, 0.001, and 0.001, respectively). Expression of P53, …

From Geology To Art History: Ceramist Alexandre Brongniart’S Overlooked Contribution To The Developing Science Of Art History In The Early Nineteenth Century, Julia A. Carr-Trebelhorn

Theses and Dissertations--Art and Visual Studies

Alexandre Brongniart was known for his work as an important geologist and as an administrator at the Sèvres Porcelain Manufactory, but his roles as art historian and museologist are overlooked. Brongniart created a holistic methodology taken directly from science and applied it to ceramic art of all cultures and eras. He had a uniquely modern perspective on time, world culture, and archeology. Brongniart wrote about the art of Asia and the Americas on an equal status with that of the Classical West at least fifty years before it became a mainstream idea. Brongniart integrated scientific principle and practice into the …

Emotional Dysregulation And Borderline Personality Disorder: Explaining The Link Between Secondary Psychopathy And Alexithymia, Leigh Elizabeth Ridings, Catherine J. Lutz-Zois

Psychology Faculty Publications

Research explaining the overlap between psychopathy and alexithymia is in its infancy. A study by Lander, Lutz-Zois, Rye, and Goodnight (2012) revealed a significant positive correlation between secondary, but not primary, psychopathy and alexithymia. However, little is known about what accounts for this differential association. Because both alexithymia (Webb & McMurran, 2008) and secondary psychopathy (Blackburn, 1996) have been linked to Borderline Personality Disorder (BPD), the current study sought to determine if emotional processing deficits characteristic of BPD could explain the link between secondary psychopathy and alexithymia. The results supported the hypothesis that BPD would mediate the association between secondary …

Institutional Repository, Ucf Libraries

Technology Fee Funded Projects

The UCF Libraries seeks $240,077 in funding to purchase a 3 year license for Digital Commons, a digital repository platform for UCF-produced digital scholarship.

Phytochemical Investigation Of The Traditional Botanical Medicine Iresine Celosia, Caroline Grace Killian

Open Access Dissertations

Iresine celosia L. is a traditional medicine used by the indigenous Mayan people for a variety of ailments. It is also the sole active ingredient in the Odyliresin™ formulation currently marketed to humans worldwide as an antioxidant and for the promotion of prostate health. However, Odyliresin™ has not been characterized phytochemically, or evaluated biologically for its intended use. This work represents the first comprehensive phytochemical investigation of the Odyliresin™ formulation botanical extract. To better understand its constitutive phytochemistry, a tailored isolation scheme was developed, using various chromatography resins (silica gel, LH-20), separation and purification techniques. In all, eleven compounds were …

Chemical Constituents Of Ochreinauclea Maingayii (Hook.F.) Ridsd. (Rubiaceae) / Norfaizah Osman, Norfaizah Osman

Student Works (2010-2019)

A study of chemical constituents from the leaves and bark of Ochreinauclea maingayii (Hook. f.) Ridsd. from the family Rubiaceae has been carried out. Nine compounds were successfully isolated and purified by applying chromatography techniques such as column chromatography (CC), thin layer chromatography (TLC) and preparative thin layer chromatography (PTLC). The compounds were harmane 38, naucledine 5, cadamine 42, isodihydrocadambine 78, neonaucline 79, cinnamide 81, benzamide 80, blumenol A 75 and ursolic acid 73. Neonaucline 75 is a new compound from the leaves of this species. All structures were elucidated by the means of spectroscopic method; 1H, 13C NMR, 2D …

Synthesis, Characterization And Biological Studies Of Nickel(Ii) Complexes Containing Thiosemicarbazone And Thiourea Derivatives / Hana Bashir Mohammed Shawish, Hana Bashir Mohammed Shawish

Student Works (2010-2019)

The aim of this study is the preparation of new nickel(II) complexes with N/S donor ligands and their mixed complexes with co-ligands. A number of nickel(II) complexes of thiosemicarbazone derivatives of 2,3-dihydroxybenzaldehyde and thiourea derivatives of diamines have been synthesized and characterized by a combination of spectroscopic studies, elemental analysis, and X-ray crystallography. Five series of nickel(II) complexes with thiosemicarbazone ligands of the type H3L (RCH=NNHC = 2,3-dihyd = H; H3L1, CH3; H3L2, C6H5; H3L3, C2H5; H3L4) have been verified by means of IR, NMR and, in some cases, X-ray crystallography. These five series of nickel(II) thiosemicarbazone complexes have been …

The Role Of Rosette Formation In Pathogenesis Of Vivax Malaria / Lee Wenn Chyau, Wenn Chyau Lee

Student Works (2010-2019)

Rosette formation is one of the unique biological phenomena that have been linked to pathobiology of malaria. It is believe to be associated with the severe outcomes of falciparum malaria. Most of the knowledge about rosetting is obtained from in-depth studies conducted on Plasmodium falciparum. However, the rosetting phenomenon and pathobiology of vivax malaria is not well studied. This research project aimed at deciphering the unknown aspects behind rosetting phenomenon of P. vivax, and investigating the role of rosette formation in pathobiology of vivax malaria. In total, 121 fresh P. vivax isolates, 48 cryopreserved P. vivax isolates, 122 fresh P. …

Using Stable Isotope Analysis To Study Altitudinal And Latitudinal Bat Migration, Lily Arias

All-Inclusive List of Electronic Theses and Dissertations

The general lack of knowledge on basic aspects of the biology of temperate and tropical bats, their low reproductive rates, and threats such as white nose syndrome, wind farms, and habitat loss, make them very susceptible to population declines.My research uses an innovative technique, the analysis of stable isotopes, to study the ecology of bat migration with the main goals of contributing significantly to the understanding of bat biology and assessing the conservation status and susceptibility of bats. In the first chapter,I measured the content of hydrogen isotopes in fur samples of migratory bat species killed at a wind farm …

Chronic Toxicity Of Nano Metallics On Red Swamp Crayfish (Procambarus Clarkii) In Laboratory And Mesocosm Studies, Jake Andrew Farlow

LSU Master's Theses

Nanotechnology has become integrated in commercial, industrial and medical products, and its use has grown exponentially in the past several years. Although potential applications of nanoparticles (NPs) are numerous, concerns about their water quality, environmental, and human health impacts remain unclear. Crayfish are ubiquitous to streams and wetland habitats, are used as a food source, and inhabit areas that could be impacted by water quality issues. Numerous studies have been conducted on the toxicity of various classes of agricultural pesticides and oils to crayfish (Procambarus clarkii) as a non-target organism. However, there is little evidence published on chronic toxicity of …

Physical Stability Of Poly (Lactic-Co-Glycolic Acid) And Plga/Chitosan Nanoparticles And Chemical Stability Of Entrapped Alpha-Tocopherol And Lutein, Meocha Whaley

LSU Master's Theses

The published benefits of polymeric nanoparticles as a system for antioxidant delivery have encompassed topics of improved oral delivery, bioavailability, and modified release to name a few. Generally accepted as safe polymers Poly(lactic-coglycolic acid) and the mucoadhesive chitosan were selected to entrap alpha-tocopherol and lutein. This study proposed to assess the physical stability of PLGA and Chi/PLGA nanoparticles with entrapped bioactives in varying biological suspension medias ( water, pbs, hbss and cell media). Also, to assess the chemical stability of the entrapped antioxidants lutein and alpha-tocopherol within the same media as deemed relevant for in-vitro and and in-vivo studies. Of …

Development Of Confocal Imaging Techniques For Probing Interfacial Dynamics In Microscale, Gas-Liquid, Two-Phase Flow, Joseph E. Hernandez

Dissertations, Master's Theses and Master's Reports - Open

Micro-scale, two-phase flow is found in a variety of devices such as Lab-on-a-chip, bio-chips, micro-heat exchangers, and fuel cells. Knowledge of the fluid behavior near the dynamic gas-liquid interface is required for developing accurate predictive models. Light is distorted near a curved gas-liquid interface preventing accurate measurement of interfacial shape and internal liquid velocities. This research focused on the development of experimental methods designed to isolate and probe dynamic liquid films and measure velocity fields near a moving gas-liquid interface. A high-speed, reflectance, swept-field confocal (RSFC) imaging system was developed for imaging near curved surfaces.

Experimental studies of dynamic gas-liquid …

Cranberry Metabolites With Urinary Anti-Adhesion Activity, Christina M. Coleman

Electronic Theses and Dissertations

The fruit of the American cranberry (Vaccinium macrocarpon Aiton, Ericaceae) is a comfood and a top selling dietary supplement in the U.S. Cranberry juice is traditionally used for the prevention of urinary tract infections (UTIs), many of which are caused by P-fimbriated Escherichia coli. Human urine produced after cranberry juice consumption can prevent E. coli adhesion, but the urinary metabolites responsible for this activity are currently not known. High-molecular weight components of cranberry juice, specifically proanthocyanidin oligomers (PACs), are currently believed to be responsible for the ability of cranberry juice to prevent UTIs. Cranberry PACs can prevent the adherence of …

Structure, Synthesis And Biological Activity Of Harmful Algal Bloom Toxins From Karlodinium Sp. And Exploration Of Microbial Derived Natural Products, Amanda Leigh Waters

Electronic Theses and Dissertations

This work features natural products isolated from both the marine and microbial environments. Part I focuses on the elucidation of toxins from harmful algal blooms (HAB). The HAB dinoflagellate Karlodinium veneficum produces a harmful suite of molecules identified as the karlotoxins (KmTx) that have been implicated in massive fish kills pan-globally. Nine new karlotoxin congeners were isolated from various Karlodinium blooms around the world and utilizing the structure of KmTx2 were characterized using overlaid 2D NMR techniques. Due to the contrasting conformational differences between KmTx and the closely related amphidinols, the absolute configuration of KmTx was further evaluated by extensive …

Junctional Sarcoplasmic Reticulum Protein Processing And Trafficking In Cardiac Tissue And Primary Cultured Cardiomyocytes, Naama Sleiman

Wayne State University Dissertations

Junctional SR is an important and unique ER subdomain in the adult myocyte that releases Ca2+ through the actions of an exclusive set of resident proteins. Cardiac calsequestrin (CSQ2) undergoes two co-translational modifications: N-linked glycosylation on 316Asn, and phosphorylation by protein kinase CK2 on a cluster of 3 serines in its tail. In the heart, CSQ2 molecules undergo extensive mannose trimming by ER mannosidase(s), a posttranslational process that often regulates protein breakdown. To investigate CSQ2 protein processing in cardiomyopathy models, studies were performed to test whether CSQ2 glycan structures would be altered in heart tissue from mongrel dogs induced into …

Identification Of A Fourth Haplotype Of Bactericera Cockerelli (Hemiptera: Triozidae) In The United States, Kylie D. Swisher, Donald C. Henne, James M. Crosslin

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The potato psyllid, Bactericera cockerelli (Sulc) (Hemiptera: Triozidae), is a pest of potato and other solanaceous crops in North and Central America and New Zealand. Previous genotyping studies have demonstrated the presence of three different haplotypes of B. cockerelli in the United States corresponding to three geographical regions: Central,Western, and Northwestern. These studies utilized psyllids collected in the western and central United States between 1998 and 2011. In an effort to further genotype potato psyllids collected in the 2012 growing season, a fourth B. cockerelli haplotype was discovered corresponding to the Southwestern United States geographical region. High-resolution melting analyses identified …

Influences Of Climate Variability And Landscape Modifications On Water Dynamics, Community Structure, And Amphipod Populations In Large Prairie Wetlands: Implications For Waterbird Conservation, Mark Thomas Wiltermuth

Environmental and Conservation Sciences

Northern prairie pothole wetlands provide crucial habitat for numerous waterbirds. However, wetland abundance and quality in the Prairie Pothole Region of North America has declined because of agricultural landscape modifications. Effective management of waterbird populations relies on understanding how landscape modifications alter wetland hydrology and biological communities in context of climate-driven wet-dry periods. A common modification involves consolidation of smaller more-temporary wetlands into larger more-permanent ones. I evaluated whether consolidation drainage has progressive-chronic effects on hydrology of remaining wetlands during 2003-2010 in the Prairie Pothole Region of North Dakota. For wetlands in topographic basins that were not already full, rate …

Egonet: Identification Of Human Disease Ego-Network Modules, Rendong Yang, Yun Bai, Zhaohui Qin, Tianwei Yu

PCOM Scholarly Works

Background: Mining novel biomarkers from gene expression profiles for accurate disease classification is challenging due to small sample size and high noise in gene expression measurements. Several studies have proposed integrated analyses of microarray data and protein-protein interaction (PPI) networks to find diagnostic subnetwork markers. However, the neighborhood relationship among network member genes has not been fully considered by those methods, leaving many potential gene markers unidentified. The main idea of this study is to take full advantage of the biological observation that genes associated with the same or similar diseases commonly reside in the same neighborhood of molecular networks.Results: …

The Emerging Field Of Geogenomics: Constraining Geological Problems With Genetic Data, Paul A. Baker, Sherilyn C. Fritz, Christopher W. Dick, Andrew J. Eckert, Brian K. Horton, Stefano Manzoni, Camila C. Ribas, Carmala N. Garzione, David S. Battisti

Department of Earth and Atmospheric Sciences: Faculty Publications

The development of a genomics-derived discipline within geology is timely, as a result of major advances in acquiring and processing geologically relevant genetic data. This paper articulates the emerging field of “geogenomics,” which involves the use of large-scale genetic data to constrain geological hypotheses. The paper introduces geogenomics and discusses how hypotheses can be ad-dressed through collaboration between geologists and evolutionary biologists. As an example, geogenomic methods are applied to evaluate competing hypotheses regarding the timing of the Andean uplift, the closure of the Isthmus of Panama, the onset of trans-Amazon drainage, and Quaternary climate variation in the Neotropics.