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The Teaching Self-Reflection Tool And Skills Checklist, Arlene Lorch, Otd, Otr/L, Ches

Department of Occupational Therapy Faculty Papers

OBJECTIVE.

To provide occupational therapy faculty with a collaborative system to review and improve teaching skills within the educational program.

METHOD.

Six faculty members completed a self-assessment using self-reflection tools and feedback from an interview with a fellow faculty member serving as consultant. Surveys evaluated perceptions of the experience.

RESULTS.

Survey responses indicated strong approval of the peer review process as a valuable method for faculty development. Results identified aspects of the process that needed revision, specifically, the burden of time required to complete the process.

CONCLUSION.

Findings support the need for a peer review process because it benefits faculty, …

Enhanced Dna Sensing Via Catalytic Aggregation Of Gold Nanoparticles, Herbert M. Huttanus, Elton Graugnard, Bernard Yurke, William B. Knowlton, Wan Kuang, William L. Hughes, Jeunghoon Lee

Materials Science and Engineering Faculty Publications and Presentations

A catalytic colorimetric detection scheme that incorporates a DNA-based hybridization chain reaction into gold nanoparticles was designed and tested. While direct aggregation forms an inter-particle linkagefrom only one target DNA strand, catalytic aggregation forms multiple linkages from a single target DNA strand. Gold nanoparticles were functionalized with thiol-modified DNA strands capable of undergoing hybridization chain reactions. The changes in their absorption spectra were measured at different times and target concentrations and compared against direct aggregation. Catalytic aggregation showed a multifold increase in sensitivity at low target concentrations when compared to direct aggregation. Gelelectrophoresis was performed to compare DNA hybridization reactions …

An Effective Color-Difference-Based Demosaicking Method, Yea-Shuan Huang, Sheng-Yi Cheng

Journal of Marine Science and Technology–Taiwan

This paper proposes an effective color-difference-based (ECDB) interpolation algorithm for color filter array (CFA) image demosaicking. A CFA image consists of a set of spectrally selective filters which are arranged in an interleaved pattern such that only one color component is sampled at each pixel location. To improve the quality of reconstructed fullcolor images from CFA images, the ECDB algorithm first analyzes the samples immediately around a missing green pixel to determine suitable samples for interpolating the value of this missing green pixel. After finishing the interpolation operations of all the missing green pixels, a complete green plane (i.e G …

Vehicle Counting Without Background Modeling, Cheng-Chang Lien, Cheng-Ta Hsieh, Ming-Hsiu Tsai

Journal of Marine Science and Technology–Taiwan

In general, the vision-based methods for vehicle detection/tracking may face the problems of illumination variation, shadows, or swaying trees. In this study, we propose a novel vehicle detection method without background modeling to overcome the aforementioned problems. First, a modified block-based frame differential method is established to quickly detect the moving vehicles without the influences of rapid illumination variations. Second, the precise vehicles’ regions are extracted with the dual foregrounds fusion method. Third, a texture-based object segmentation method is proposed to segment each vehicle from the merged foreground image blob and remove the shadows. Fourth, based on the concept of …

Determining The Internal Structure Of Cement-Based Materials By Using Mri Techniques, Hsiang-Wei Chiang, Ran Huang, Chia-Jung Tsai

Journal of Marine Science and Technology–Taiwan

The primary concerns of the internal structure of cementbased materials involve the distribution of pores, pore size, and the manner in which they are connected. Thoroughly understanding the distributive rules of pores and the approaches to breaking their links can contribute considerably to enhancing the durability of cement-based materials. Several methods are used to measure the pores of cement-based materials; however, they cannot determine the exact location of pores or structural links. Magnetic resonance imaging (MRI) has been widely applied in physical examinations and has matured to the level of being able to obtain 3D images of the organs of …

Fishing Vs. Climate Change: An Example Of Filefish (Thamnaconus Modestus) In The Northern East China Sea, Sukgeun Jung, Hyung Kee Cha

Journal of Marine Science and Technology–Taiwan

The main cause of annual fluctuations in catch and species composition of fisheries is usually uncertain, but a prevailing view has been that fishing effects are more critical than environmental variability. Filefish is a good anecdote: many Korean fisheries scientists have attributed the sudden collapse of Korean filefish fisheries in the early 1990s to overfishing, especially by trawl fisheries in the northern East China Sea (NECS). However, interdisciplinary researches have revealed that climate-driven, multi-decadal variability in oceanic conditions impacts both fish and fisheries around the world. To test the two alternative hypotheses (i.e., fishing and climate) as the major cause …

Seasonal Dynamics Of Flatfish Assemblage In Coastal Waters Off Northeastern Taiwan: With Implications For Shift In Species Dominance Of The Ecosystem, Shyh-Bin Wang, Wen-Kwen Chen, Shoou-Jeng Joung

Journal of Marine Science and Technology–Taiwan

The seasonal dynamics of flatfish assemblage in the coastal waters off northeastern Taiwan was examined based on samples collected between March 2004 and March 2005 taken by bottom trawlers and landed at Da-Shi fishing port, Yilan county. A total of 28 species from 5 families and 18 genera were found. The Bothidae was the most dominant family, accounting for 81% of the total catch, while the most dominant species was the Pseudorhombus pentophthalmus. Species richness and diversity were high between the end of fall and the end of spring, but low in summer. Multivariate analyses based on cluster and nonparametric …

Age And Growth Study Of The Jack Mackerel (Trachurus Japonicus) In The Northeatern Waters Off Taiwan, Hsueh-Jung Lu, Chih-Chun Chen, Jen-Chia Cheng

Journal of Marine Science and Technology–Taiwan

Jack mackerel (Trachurus japonicus) is one of the most important fish resources in the northeastern Taiwan. Because of the flourishing of Taiwanese seiner fisheries in recent years, the proportion and catch of jack mackerels have increased. However, the studies on their age and growth are outdated, thus data on the catch-at-age composition are lacking. The present study collected jack mackerel otolith samples from May, 2010, to April, 2011, for age determination and combined those data with length and weight data from 2005 to January, 2012, to investigate the catch-at-age of jack mackerel in the northeastern waters off Taiwan. The regression …

Summer Assemblages Of Ichthyoplankton In The Waters Of The East China Sea Shelf And Around Taiwan In 2007, Ming-An Lee, Yi-Chen Wang, Yu-Kai Chen, Wen-Yu Chen, Long-Jing Wu, Don-Chung Liu, Ji-Lun Wu, Sheng-Yuan Teng

Journal of Marine Science and Technology–Taiwan

This study investigated the relationships between assemblages of larval fish and hydrographic features in the East China Sea (ECS) and seas surrounding Taiwan during the summer of 2007. A survey was conducted from 2007 July 1 to 2007 July 11 in the ECS and from 2007 July 4 to 2007 July 16 in the waters surrounding Taiwan. A total of 12,670 larval fishes belonging to 95 families, 163 genes, and 189 species were identified. Engraulis japonicus and Sillago japonica were the 2 dominant species, accounting for 56.15% and 6.66% of the fish larvae samples. The cluster analysis results showed that …

Application Of Lc-Ms/Ms In Identification Of Toxin In The Causative Gastropod And Victim Samples, Chun-Lan Lin, Cheng-Hong Hsieh, You-Liang Hsieh, Yi-Cheng Tai, Deng-Fwu Hwang

Journal of Marine Science and Technology–Taiwan

Food paralytic poisoning incident was detected in the gastropod Nassariidae Niotha clathrata and Zeuxis scalaris occurred in December 2012 in Kaohsiung, Taiwan. The toxin was purified from each remain of cooked gastropod and victim tissues by ultrafiltration using a YM-3 membrane, followed by C18 cartridge column purification. Based on analyses by LC-MS/MS, the causative agent of gastropod food poisoning was identified as tetrodotoxin. The toxicity of remain of cooked sample was 245 MU/g (1MU = 0.178 µg TTX) for N. clathrata and 203 MU/g for Z. scalaris. The blood and excrements of victim was shown to contain TTX of 0.13 …

A Comparative Study Between Ranavirus And Megalocytivirus Infections In Orange-Spotted Grouper (Epienphelus Coioides), Jiann-Horng Leu, Ming-Hung Wu, Hsin-Yiu Chou

Journal of Marine Science and Technology–Taiwan

Piscine iridoviruses infect a wide variety of fish and are classified into three genera: Lymphocystivirus, Ranavirus and Megalocytivirus. Lymphocystiviruses cause non-fatal, dermal infections, while ranaviruses and megalocytiviruses produce devastating, systemic infections with mortality reaching up to 100%. Although both ranaviruses and megalocytiviruses cause fatal systemic infections, they induce different pathology. In Taiwan, both ranaviruses and megalocytiviruses have caused serious epidemics in several mariculture fish species, including groupers. In this study, we infected the orange-spotted grouper (Epinephelus coioides) with either a megalocytivirus (TGIV, grouper iridovirus of Taiwan) or a ranavirus (GIV, grouper iridovirus), and then the two iridoviruses were investigated and …

Ichthyoplankton Community Associated With Oceanic Fronts In Early Winter On The Continental Shelf Of The Southern East China Sea, Yi-Chen Wang, Wen-Yu Chen, Yi Chang, Ming-An Lee

Journal of Marine Science and Technology–Taiwan

The study of ichthyoplankton community associated with oceanic features on the continental shelf of southern East China Sea (ECS) was conducted in early winter from 2006 to 2009. The species composition of the ichthyoplankton and its spatial distribution was classified and compared with oceanic front. A total number of 1,809 fish larvae individuals were sampled from 5 field surveys, and represented 76 families and 137 species. Using the Bray-Curtis dissimilarity, the abundance of species found to exceed 0.5% of the total sample from 36 stations were classified into four groups: China Coastal Group, Mixed Shelf Group, Taiwan Strait Group, and …

Comparative Morphological Study Of Otoliths In Taiwanese Anguilliformes Fishes, Abbie K. Chulin, Hong-Ming Chen

Journal of Marine Science and Technology–Taiwan

Otoliths are calcareous structures found in the labyrinth membrane in the otic capsules of teleost fishes and are constituted of three pairs: the sagittae, lapilli and asterisci. They have long been recognized as being species specific. A comparative morphology of the sagittae, asterisci and lapilli among six eel species of Anguilliformes belonging to the Family Congridae, Muraenesocidae and Muraenidae was studied. A total of 64 specimens belonging to Bathyconger wallacei (n = 23), Conger japonicus (n = 9), Muraenesox cinereus (n = 10), Echidna polyzona (n = 4), Gymnothorax eurostus (n = 4) and Gymnothorax reticularis (n = 14) were …

The Molecular Phylogeny Of Genus Hemigobius (Teleostei: Gobiidae), With The Comfirmation Of Validity Of Hemigobius Crassa (Herre, 1945), Shih-Pin Huang, I-Shiung Chen, Kwang-Tsao Shao

Journal of Marine Science and Technology–Taiwan

The genus Hemigobius Bleeker, 1874 is has been regarded as only two valid species in previous study including H. hoevenii (Bleeker, 1851) and H. mingi (Herre, 1936). Traditionally, the both Vaimosa crassa Herre, 1945 and Mugilogobius obliquifasciatus Wu and Ni, 1985 found from Chinese and Taiwanese waters had ever been regarded as the junior synonyms of H. hoevenii. However, our present study have been confirm that H. crassa collected from Hong Kong and Taiwan is a valid species. The H. crassa can be well distinguished from H. hoevenii based on several morphological features. The molecular phylogenetic analysis firstly based on …

Redescription Of Gobius Gastrospilos Bleeker, 1853 With Comments On Four Newly Recorded Species Of Brackish Gobies From Taiwan, Shih-Pin Huang, Martien J. P. Van Oijen, Kuang-Ying Huang, Chin-Chu Tsai, I-Shiung Chen

Journal of Marine Science and Technology–Taiwan

A re-examination of the holotype of Gobius gastrospilos Bleeker, 1853 confirmed thatthis species belongs to the genus Pseudogobius Popta, 1922. The detailed redescription of Pseudogobius gastrospilos (Bleeker, 1853) would be provided in this paper. Furthermore, a series of field surveys of the gobioid fauna in brackish habitats of Taiwan revealed and summarized the following four newly recorded gobiid species: Pseudogobius gastrospilos (Bleeker, 1853), Mugilogobius chulae (Smith, 1932), Mugilogobius mertoni (Weber, 1911) and Mugilogobius myxodermus (Herre, 1935). The diagnosis of these newly recorded species of Taiwan would be also provided herein.

An Annotated Checklist Of Gobioid Fishes From The Mangrove Estuary Of Matang, Malay Peninsula, With Comments On A New Pseudogobius (Teleostei: Gobiidae) Species, Shih-Pin Huang, Kwang-Tsao Shao, Hao-Ming Huang, Ving-Ching Chong, I-Shiung Chen

Journal of Marine Science and Technology–Taiwan

A series of field collections of fishes were made in the Matang mangrove, Malay Peninsula in April 2011. At least 23 genera and 27 species of gobioid fishes including a new Pseudogobius species were collected and recorded in this region during the expedition. The checklist of gobioid fauna and available materials are provided herein, and the formal description of a new goby species, Pseudogobius fulvicaudus Huang, Shao & Chen, new species will be provided and discussed in this paper.

A New Freshwater Goby Of Rhinogobius (Teleostei: Gobiidae) From Hainan Island, Southern China, I-Shiung Chen, Peter J. Miller

Journal of Marine Science and Technology–Taiwan

A new freshwater goby of Rhinogobius Gill (1859) was collected from the Wangchuang River basin, Hainan Island, southern China. The new species, Rhinogobius sangenloensis, can be well distinguished from other congeners by a combination of the following features: second dorsal-fin rays modally I/8; anal-fin rays I/7-8; pectoral-fin rays modally 17; longitudinal scales 25-27; predorsal scales 9-11; vertebrae 26; body with 3-4 longitudinal, discontinuous orange to brown or brownish black bars and stripes; cheek with 2 grayish black spots in male; pectoral-fin base in male with 2 oblong black bars; caudal fin with an orange mark on lower 1/3 region in …

A New Species Of Genus Pseudogobius Popta (Teleostei: Gobiidae) From Brackish Water Of Taiwan And Southern China, I-Shiung Chen, Shih-Pin Huang, Kuang-Ying Huang

Journal of Marine Science and Technology–Taiwan

A new species of fat-nose goby genus Pseudogobius taijiangensis n. sp. is firstly described herein and was collected from brackish waters of Taiwan and southern China. P. taijiangensis n. sp. can be well distinguished from other congeners by the unique combinations of the following features: (1) fin rays: 2nd dorsal fin rays modally 7, anal fin rays modally 7, pectoral fin rays modally 17 and first dorsal fin rounded and lacking filaments; (2) squamation: lateral body with large ctenoid scales, longitudinal scale rows 26-28, predorsal scales modally 8; and (3) specific coloration: body with some black thin longitudinal lines; rear …

A New Species Of Cyprinid Fish Of Genus Opsariichthys From Ky Cung-Bang Giang River Basin, Northern Vietnam With Notes On The Taxonomic Status Of The Genus From Northern Vietnam And Southern China, Thien Quang Huynh, I-Shiung Chen

Journal of Marine Science and Technology–Taiwan

Opsariichthys duchuunguyeni, a new species of cyprinid fish from Ky Cung – Bang Giang river basin, northern Vietnam, is described. It is characterized by the following unique combination of features: short maxillary extending; 12-15 rounded tubercles on lower jaw; 11-12 crossbars on body; pectoral fin reaching the pelvic fin origin in adult male; lateral-line scales comprising 41; scales above lateral-line 8; scales below lateral-line 3. Moreover, the genetic differentiation and a diagnostic key of the new species and other congeners are given in this paper for discussion about taxonomic status of the genus Opsariichthys from Vietnam and nearby geographical regions, …

Seasonal Abundance Of Epiphytic Dinoflagellates Around Coastal Waters Of Jeju Island, Korea, Md. Mahfuzur Rahman Shah, So-Jeong An, Joon-Baek Lee

Journal of Marine Science and Technology–Taiwan

The seasonal abundance of epiphytic dinoflagellates in the intertidal zone of Jeju Island, Korea was quantitatively estimated by monthly collection of macroalgal samples (Rhodophyta, Phaeophyta, and Chlorophyta) from six sampling locations from July 2012 to June 2013. Ten epiphytic dinoflagellate taxa, including eight potentially toxic species Amphidinium carterae, A. operculatum, Gambierdiscus sp., Ostreopsis ovata, Prorocentrum concavum, P. emarginatum, P. lima, and P. rhathymum, were identified. Two Amphidinium and three Prorocentrum species are newly recorded in Korean coastal waters. A significant change in seasonal abundance was recorded with maximum (751.82 ± 223.12 cells g-1 wet weight of algae; cells g-1 hereafter) …

Taxonomic Study Of Pike Congers (Anguilliformes: Muraenesocidae) With Identification Of Muraenesocid Collections In Taiwan, James Lin, Kwang-Tsao Shao, Hong-Ming Chen

Journal of Marine Science and Technology–Taiwan

During the past years, we collected and studied on the Anguilliformes fishes, and described some new genera and new species of eels. We noticed that there are some misidentified species or invalid junior synonym in Muraenesocidae specimens. Considering how limited this family is known in Taiwan, we would like to revise the species of pike congers here. We collected muraenesocid specimens around Taiwan from fish markets, fishery harbors, fishermen, and also examined the specimens from other Taiwanese museum collections. Comparing to the past literature of Muraenesocidae, we found that the species Congresox talabon was misreported previously, and there is not …

Genetic Diversity Of Filamentous Cyanobacteria From Shore Regions Of Okinawa, Shoichiro Suda, Rui Moriya, Shinpei Sumimoto, Osamu Ohno, Kiyotake Suenaga

Journal of Marine Science and Technology–Taiwan

Benthic marine cyanobacteria are ubiquitous oxygenic photosynthetic bacteria and they form major components of various communities, including epiphytic, epilithic and microbial mats, in coral reef environments. Filamentous groups may form almost monospecific macroscopic colonies, and sometimes make potentially harmful algal blooms (HABs). HABs bring serious problems for marine tourism but marine filamentous cyanobacteria are good resource for drug discovery. However, cultivation and identification of such filamentous cyanobacteria are difficult and there are taxonomic confusions. In this study, we investigate 17 samples from coral reef area of 10 localities in Okinawa. Macroscopic mats or cushions of filamentous cyanobacteria were collected. The …

Diversity Of Crypthecodinium Spp. (Dinophyceae) From Okinawa Prefecture, Japan, Danang Ambar Prabowo, Ooshi Hiraishi, Shoichiro Suda

Journal of Marine Science and Technology–Taiwan

The genus Crypthecodinium (Dinophyceae) currently consists of only one species: C. cohnii, a heterotrophic marine dinoflagellate widely known able to produce prolific amount of DHA. However, previous studies revealed that there are morphological and genetic differences among Crypthedodinium cohnii-like strains, indicating the potential of undiscovered diversity of this dinoflagellate. Attempts of isolating heterotrophic marine dinoflagellate strains were made from submerged mangrove leaves and seaweed. A total of nine strains were established, cultured and maintained using seawater-based GTY medium for taxonomic identification and phylogenetic analyses based on SSU, ITS1, 5.8S, ITS2 and LSU rDNA. Morphological observation revealed all strains shared similar …

Effects Of Elevated Seawater Co2 On Feed Intake, Oxygen Consumption And Morphology Of Aristotle's Lantern In The Sea Urchin Anthocidaris Crassispina, Guining Wang, Mitsuharu Yagi, Rui Yin, Weiqun Lu, Atsushi Ishimatsu

Journal of Marine Science and Technology–Taiwan

Adult sea urchins, Anthocidaris crassispina, were reared individually in running seawater equilibrated with gas mixtures containing 380 ppm (control), 1,000 ppm or 3,000 ppm CO2, at ambient natural temperature for 140 days to study effects of elevated CO2 on feed intake, fecal production, oxygen consumption, and the morphology of Aristotle’s lantern. Feed intake became significantly lower in the two high CO2 groups than in the control after 90 (1,000 ppm) or 110 (3,000 ppm) days and remained suppressed until the end of the experiment (control, 0.16 ± 0.01 (mean ± SE) g dry wt ind-1 day-1; 1,000 ppm, 0.10 ± …

One New Record Genus And Three New Record Species Of Snake Eels (Ophichthidae:Anguilliformes) From Taiwan, Yung-Chieh Chiu, James Lin, Hong-Ming Chen

Journal of Marine Science and Technology–Taiwan

The tropical and subtropical eel family Ophichthidae are the most diverse Anguilliformes fishes which occupy a variety of marine habitats such as reefs, sand and mud substrates. In Taiwan, we can often collect them from the capture of bottom-trawl fishery. In this study, we added two new recorded species Ophichthus machidai and O. obtusus of ophichthid eels collected from Da-xi fish market by comparing their morphological measurements, vertebral counts and head pores with the other six species of the genus Ophichthus: O. apicalis, O. aphotistos, O. asakusae, O. erabo, O. tsuchidae, O. urolophus. We also obtained a unique specimen from …

Reproductive Biology Of Intertidal Frillfin Goby, Bathygoboius Fuscus In Keelung, Taiwan, Yu-Hai Kong, I-Shiung Chen

Journal of Marine Science and Technology–Taiwan

The reproductive biology of intertidal, black brown frillfin goby, Bathygobius fuscus (Rüppell) have been surveyed from the coastal region of Keelung, Taiwan. The species always belongs to the dominant species found in the fish community of the tidal pools for different seasons. The overall fecundity of adult female is from 2335 to 13332 eggs with the average of 5605 eggs with average eggs-size is 0.34 ± 0.06 mm. The female minimum measure body-size would be 40.4 mm SL. The higher reproductive season can be well observed from female GSI (Gonadosomatic Index) during May to September. During that time, there may …

Alternative Assessment Methods Applied To The Hairtail (Trichiurus Lepturus) Stock In The Aru Sea As An Example, Chao-Hsiung Cheng, Tsuyoshi Kawasaki, Kuo-Ping Chiang, Chuan-Hung Ho

Journal of Marine Science and Technology–Taiwan

The Aru Sea off Indonesia is a major fishing ground for Taiwanese distant-water otter trawlers. The hairtail, an economically important demersal fish, is abundant in that area. The assessment methodology in this paper extends previous studies on hairtail fisheries by considering migration within the fishing grounds. This model is based on conventional Leslie-Delury method but makes different assumptions on stock movement and the relationship between stock abundance and catch per unit effort. It was applied to retrospective data from the hairtail fisheries in the Aru Sea. In addition, the depletion method was used to estimate the abundance of hairtail and …

A New Parapercis Species From The Dongsha Island, South China Sea With Comments On A New Record From Taiwan, I-Shiung Chen, Tsung-Hsien Tsai, Shao-Liang Hsu

Journal of Marine Science and Technology–Taiwan

The sandperch genus, Parapercis Bleeker, 1863 is the most diverse group of Family Pinguipedidae in the perciforms. One unusual specimen of sandperches recently collected from the Dongsha Island (Pratas Island), South China Sea from 2011 expedition belonging to Taiwan, the Republic of China is turned to a new species as Parapercis dongshaensis n. sp. closely related to the newly recorded species, Parapercis okamurai Kamohara, 1960 which also firstly collected from Taiwanese waters would been also documented herein. The new species, Parapercis dongshaensis n. sp., can be well distinguished from other congeners by the following unique combination of features: (1) fins: …

Identification And Analysis Of White Spot Syndrome Virus (Wssv) Envelope Protein Vp41a, Po-Yu Huang, Pei-Jung Chen, Huai-Ting Huang, Li-Li Chen

Journal of Marine Science and Technology–Taiwan

White spot syndrome virus (WSSV) is the causative agent of a disease that has led to severe mortalities of cultured shrimp in Taiwan and many other countries. WSSV, a kind of large enveloped DNA virus, has a wide host range among crustaceans. During the first stage to infect a target cell, the enveloped virus must combine itself with the receptor on the host cell membrane via an envelope protein. In WSSV, binding of envelope protein with the receptor of the host cell membrane was revealed in a number of previous studies. WSSV envelope proteins were also considered to form a …

How Important Is Viral Effect On The Measure Bacterial Growth In Western Subtropical Pacific Coastal Water?, An-Yi Tsai, Gwo-Ching Gong, Kuo-Ping Chiang, Jie Hung

Journal of Marine Science and Technology–Taiwan

Bacterial growth rates were determined in natural seawater samples using two different methodological approaches: incubation in dialysis bags, and a modification of the dilution approach. Bacterial growth rates estimated for the dialysis bag varied from 0.026 h-1 (April) to 0.208 h-1 (August). Bacterial growth rates in 0.2 µm and 30 kDa size-fractionated series ranged from 0.038 to 0.094 h-1 and 0.078 to 0.42 h-1, respectively. Furthermore, in all of our samples, growth rates in the 30 kDa fractionated series (grazer and virus-free diluent) were higher than in the 0.2 µm series (grazer-free diluent) or in the dialysis bag incubations.