Digital Commons Network^™

The Trm2h : Detecting Protein-Protein Interactions By Engineering The Cell As A Biosensor, Elyse Shimomura, Paulina Perezalonso

Bioengineering Senior Theses

Protein-protein interactions regulate key cellular functions and cell signaling pathways in the body; biological systems can be disrupted by protein interactions or lack of interactions that cause cell dysfunction and can lead to disease or illness. However, studying these interactions is complex and difficult to quantify. In this project, we examine the interaction of two putative cancer biomarkers, Bax and PDCDS, using the tetracycline repressor-based mammalian two-hybrid system (trM2H). This in vivo system engineers the cell as a biosensor using methods of DNA cloning and mammalian cell transfections that easily detects and quantities the interaction of two proteins by the …

Compassion Fatigue: Prevalence Among Nurses At A Midwest Pediatric Hospital, Branch Carole

Dissertations

Compassion fatigue is a term used to describe the unique stressors affecting people in care-giving professions (Joinson in 1992). For nurses, the impact of compassion fatigue may result in stress-related symptoms, job dissatisfaction, decreased productivity, patient satisfaction, safety issues, and job turnover. The purposes of this non-experimental descriptive survey using electronic distribution to collect data were to identify the prevalence of compassion fatigue composed of burnout and secondary traumatic stress and compassion satisfaction among nurses and nursing staff who deliver direct patient care at the hospital and the relationship between demographic variables (age, work category, level of education, work experience) …

The Use Of Forest Stand Age Information In An Atmospheric Co2 Inversion Applied To North America, Feng Deng, Jing M. Chen, Y. Pan, W. Peters, R. Birdsey, K. Mccullough, Jingfeng Xiao

All Materials

Atmospheric inversions have become an important tool in quantifying carbon dioxide (CO2) sinks and sources at a variety of spatiotemporal scales, but associated large uncertainties restrain the inversion research community from reaching agreement on many important subjects. We enhanced an atmospheric inversion of the CO2 flux for North America by introducing spatially explicit information on forest stand age for US and Canada as an additional constraint, since forest carbon dynamics are closely related to time since disturbance. To use stand age information in the inversion, we converted stand age into an age factor, and included the covariances between subcontinental regions …

Adoptive T Cell Therapy For Metastatic Colorectal Cancer, Shauna Ebanks

Summer Training Program in Cancer Immunotherapy

Colorectal cancer is the second leading cause of cancer-related death in US (1). Most of the mortality reflects local, regional and distant metastatic tumors. Cancers that are confined within the wall of the colon are often curable with surgery while tumor cells that have spread into other organs like liver and lung has low curable possibility, even though the chemotherapies and monoclonal antibodies can extend the person's life and improve quality of life(2, 3). Surgery is the main treatment for primary colorectal tumors but recurrence can happen and develop into metastasis. In addition, colorectal cancer …

Isso Weekly Newsletter, August 8, 2013, University Of Northern Iowa. International Students And Scholars Office.

ISSO Weekly Newsletter

Inside This Issue:

-- ISSO Office Has Moved
-- Live to 9 Concert Series August 9
-- Movies Under the Moon in Overman Park August 9
-- Waterloo Bucks Baseball August 8
-- ISSO Summer Office Hours
-- Things to Do in the Summer
-- Reminder to like our Facebook page
-- ISSO - Enhancing International Experiences

A Nuclear Variant Of Gdf5 May Present Novel Insights On Arthritis Formation, Michael Baldwin, Dr. Laura Bridgewater

Journal of Undergraduate Research

More than 40 million Americans suffer from the pains of arthritis. Arthritis is characterized by joint inflammation and cartilage deterioration (3). For many years growth differentiation factor 5 (Gdf5) has been associated with cartilage formation and maintenance (4, 1). GDF5 has been traditionally regarded as a secreted growth factor; however, a new variant of Gdf5 was recently discovered in the nucleus. Nuclear Gdf5 (nGdf5) may present a new mechanism by which Gdf5 regulates cartilage development and preservation.

Determining The Effect Of Toll-Like Receptor-7 On Systemic Lupus Erythematosus Development, Douglas Baumann, Dr. Brian Poole

Journal of Undergraduate Research

Systemic Lupus Erythematosus (Lupus) is an autoimmune disease that afflicts over 250,000 citizens of the United States. Although much remains to be learned about Lupus, the information gathered so far has proven to be extremely valuable in increasing survival rates and improving the quality of life of Lupus patients. As we continue to put time and energy into Lupus research, the result will be that we will be able to combat the disease more effectively. The first step in combating Lupus is to gain knowledge about the pathogenesis and mechanisms of the disease. By further developing our knowledge, we will …

Bulletin, Dmacc Marketing

Bulletin

No abstract provided.

Pengembangan Buah Manggis Sebagai Komoditas Ekspor Indonesia, Warid Ali Qosim

Jurnal Kultivasi

Mangosteen fruit is one of typical fruit and pledge Indonesia. The export demand of mangosteen fruit is always increasing every year. The increasing of mangosteen fruit production is very important to support export demand global and domestic markets. The strategies of development mangosteen fruit are essential and should be focused on increasing planting area, production and productivity of mangosteen fruit, to build mangosteen orchard SOP bases; to form mangosteen agribusiness region; to improve the competitiveness of Indonesian mangosteen products with other countries; to improve and simplify the mangosteen exports to foreign countries; to improve the investment in the area of …

Metabolomics Investigation Of Glyceollins By On-Line Liquid Chromatography-Electrospray Ionization Tandem Mass Spectrometry And Fungal Metabolite Identification By Thermal Desorption Analysis Coupled With Gas Chromatography-Mass Spectrometry, Syeda Quadri

LSU New Orleans Theses and Dissertations

Metabolomics is an emerging field that entails the detailed characterization of the ensemble of metabolites produced by living organisms; subfields include drug metabolism and natural environmental toxin production. The first part of the dissertation pursued metabolism of glyceollins, i.e., isoflavones produced by soybeans, that are potential cancer therapy agents. In vivo glyceollin metabolites produced in rats were investigated by on-line Liquid Chromatography-Electrospray Ionization Tandem Mass Spectrometry. An odd-electron fragment ion at m/z 148, formed in violation of the even-electron rule, and diagnostic of the glyceollin backbone, was discovered. Based on this finding, a negative mode precursor ion scanning method was …

Lighting The Fire: How Peer-Mentoring Helps Adult Learners Increase Their Interest In Stem Careers: A Case Study At The Community College Level, Patricia Marie Deturk

Dissertations and Theses

In the U.S., about 7,000 high school students drop out each school day, representing a loss of talent and ability. Concurrently, there are a decreasing number of enrolled students taking science-related courses at the high school and college levels. Adults, who return to obtain their General Educational Development (GED) certification, are an untapped resource that could be steered toward STEM (Science, Technology, Engineering and Mathematics) careers. In this case study, 15 GED students were shown a STEM video, and then peer mentored by 8 CLA (Clinical Laboratory Assistant) students, in a student-centered laboratory experience. Individual interviews of the GED students …

Land Blubber, Unh Communications And Public Affairs

UNH Today Archive

No abstract provided.

An Assessment Of Worldview-2 Imagery For The Classification Of A Mixed Deciduous Forest, Nahid Carter

Theses

Remote sensing provides a variety of methods for classifying forest communities and can be a valuable tool for the impact assessment of invasive species. The emerald ash borer (Agrilus planipennis) infestation of ash trees (Fraxinus) in the United States has resulted in the mortality of large stands of ash throughout the Northeast. This study assessed the suitability of multi-temporal Worldview-2 multispectral satellite imagery for classifying a mixed deciduous forest in Upstate New York. Training sites were collected using a Global Positioning System (GPS) receiver, with each training site consisting of a single tree of a corresponding class. Six classes were …

Aamodt Litigation Settlement Agreement (Pueblos Of Nambé, Pojoaque, San Ildefonso & Tesuque), United States, State Of New Mexico, Pueblo Of Tesuque, Pueblo Of San Ildefonso, Pueblo Of Nambé, Pueblo Of Pojoaque

Native American Water Rights Settlement Project

Settlement Agreement: Aamodt Litigation Settlement Agreement (Apr. 19, 2012). 66cv06639, USDC, DCNM. (final signatures Mar. 27,2013) Parties: Pueblos of Nambé, Pojoaque, San Ildefonso & Tesuque, US, NM, Santa Fe County, City of Santa Fe. The key provisions of the Aamodt settlement include: 1) constructing a Regional Water System; 2) providing non-Indians a choice of whether to join the settlement and upon joining, a choice of whether to connect to the Regional Water System for domestic water; 3) relinquishment of existing Pueblo claims against non-Indians who join the Settlement; 4) closing the Pojoaque Basin to new water right development following the …

Associations Between The Legal Context Of Hiv, Perceived Social Capital, And Hiv Antiretroviral Adherence In North America, J Craig Phillips, Allison Webel, Carol Dawson Rose, Inge B. Corless, Kathleen M. Sullivan, Joachim Voss, Dean Wantland, Kathleen Nokes, John Brion, Wei-Ti Chen, Scholastika Iipinge, Lucille Sanzero Eller, Lynda Tyer-Viola, Marta Rivero-Méndez, Patrice K. Nicholas, Mallory O. Johnson, Mary Maryland, Jeanne Kemppainen, Carmen J. Portillo, Puangtip Chaiphibalsarisdi

Publications and Research

Background Human rights approaches to manage HIV and efforts to decriminalize HIV exposure/transmission globally offer hope to persons living with HIV (PLWH). However, among vulnerable populations of PLWH, substantial human rights and structural challenges (disadvantage and injustice that results from everyday practices of a well-intentioned liberal society) must be addressed. These challenges span all ecosocial context levels and in North America (Canada and the United States) can include prosecution for HIV nondisclosure and HIV exposure/transmission. Our aims were to: 1) Determine if there were associations between the social structural factor of criminalization of HIV exposure/transmission, the individual factor of perceived …

Confocal Microscopy Study Of The Embryonic Development Of The Viviparous Nemertean Prosorhochmus Americanus Reveals Larval Features Supporting Indirect Development In Hoplonemerteans, S Tyler Spindle

Theses and Dissertations

Recent studies of hoplonenemertean planuliform larvae have clarified their development and provided insight into larval evolution within the phylum. However, an assessment of viviparous development using modern techniques is lacking. To help facilitate a comprehensive comparative evaluation of developmental diversity within hoplonemerteans, we have conducted a confocal laser scanning microscopy investigation of the development in Prosorhochmus americanus, one of the few viviparous hoplonemertean species. Phalloidin staining provides evidence of a modified transitory larval epidermis, and reveals that the foregut, midgut, proboscis, central nervous system, and body wall musculature form early in development, consistent with observations for planktonic and encapsulated hoplonemertean …

Mechanism Of Ubiquitin Ligation And Lysine Prioritization By A Hect E3, Hari B. Kamadurai, Yu Qiu, Alan Deng, Joseph S. Harrison, Chris Macdonald, Marcelo Actis, Patrick Rodrigues, Darcie J. Miller, Judith Souphron, Steven M. Lewis, Igor Kurinov, Naoaki Fujii, Michal Hammel, Robert Piper, Brian Kuhlman, Brenda A. Schulman

College of the Pacific Faculty Articles

Ubiquitination by HECT E3 enzymes regulates myriad processes, including tumor suppression, transcription, protein trafficking, and degradation. HECT E3s use a two-step mechanism to ligate ubiquitin to target proteins. The first step is guided by interactions between the catalytic HECT domain and the E2∼ubiquitin intermediate, which promote formation of a transient, thioester-bonded HECT∼ubiquitin intermediate. Here we report that the second step of ligation is mediated by a distinct catalytic architecture established by both the HECT E3 and its covalently linked ubiquitin. The structure of a chemically trapped proxy for an E3∼ubiquitin-substrate intermediate reveals three-way interactions between ubiquitin and the bilobal HECT …

Coupling S100a4 To Rhotekin Alters Rho Signaling Output In Breast Cancer Cells, Min Chen, Anne R. Bresnick, Kathleen L. O'Connor

Markey Cancer Center Faculty Publications

Rho signaling is increasingly recognized to contribute to invasion and metastasis. In this study, we discovered that metastasis-associated protein S100A4 interacts with the Rho-binding domain (RBD) of Rhotekin, thus connecting S100A4 to the Rho pathway. Glutathione S-transferase pull-down and immunoprecipitation assays demonstrated that S100A4 specifically and directly binds to Rhotekin RBD, but not the other Rho effector RBDs. S100A4 binding to Rhotekin is calcium-dependent and uses residues distinct from those bound by active Rho. Interestingly, we found that S100A4 and Rhotekin can form a complex with active RhoA. Using RNA interference, we determined that suppression of both S100A4 and …

Smart Students Study Cutting-Edge Science With Project Smart, Unh Communications And Public Affairs

UNH Today Archive

No abstract provided.

Geochemical Tracers Linking Submarine Groundwater Discharge To Hypoxia Formation In Long Bay, South Carolina, Usa, Sarah Lynn Chappell

Electronic Theses and Dissertations

Long Bay, South Carolina has experienced hypoxic conditions (<2mg/L dissolved oxygen) in the nearshore environment, not fully attributed to traditional formation mechanisms. Past research suggested physical, biological, and/or anthropogenic influences on low dissolved oxygen levels. This project aimed to determine the contribution of submarine groundwater discharge (SGD) to hypoxia formation. We measured activities of 222Rnxs, 224Raxs, 223Ra, 228Ra, and 226Ra (3.8d, 3.6d, 11.5d, 5.8yr, 1600yr half-lives, respectively) in nearshore bottom waters from April 2012 through April 2013. Radium activities observed during a hypoxic event on 16-Aug-2012 were up to an order of magnitude higher than those seen during oxic conditions and the highest ever observed in the open ocean, to our knowledge. We determined that a hypoxic water mass, comprised heavily of offshore anoxic SGD, migrated inshore due to physical conditions constraining mixing over a two week time period. High groundwater content suggests that observed hypoxic conditions in nearshore Long Bay may be independent of biological influence once offshore SGD occurs.

Mekanisme Self-Incomatibility Tipe Gametofitik Dan Sporofitik Dan Aplikasinya Dalam Pemuliaan Tanaman, Warid Ali Qosim

Jurnal Kultivasi

Self-Incomatibility (SI) is one of phenomena important on the fertile flower, but the inhibition occurs in the fertilization process, so SI caused outcrossing. The aim of this study is reveal the mechanism of self-incomatibility gametophytic and sporophytic and its application in plant breeding. The type of self-incompatibility is divided into heteromorphic and homomorphic. Type of SI heteromorphic cause a different flower structures, whereas SI homomorphic cause interaction with a single S-locus multialelic between pollen and pistil during the process of pollination and fertilization. SI heteromorphic is divided into gametophytic and sporophytic. SI gametophytic is controlled by the haploid S-alleles in …

Pembentukan Ubi Mikro Kentang Kultivar Granola Dengan Penggunaan Jenis Dan Konsentrasi Zat Inhibitor, Rifky Yusuf, Erni Suminar

Jurnal Kultivasi

The aim of the experiment was to obtain the concentration of inhibitors such as coumarin and paclobutrazol on the growth of micro-cuttings and in vitro tuberization of potato micro tuber. It can be used as source of high quality seeds.Experiments was conducted in the Tissue Culture laboratory Faculty of Agriculture, University of Padjadjaran in Jatinangor, Sumedang, from March 2011 to July 2011. Randomized Complete Design (RCD) was used in this experiment. They areconsist of 12 treatments with three replications, with treatment as follows: coumarin (30 mg L-1, 45 mg L-1, 60 mg L-1, 75 mg L-1, 90 mg L-1, 105 …

Pertumbuhan Dan Hasil Gandum (Triticum Aestivum L.) Yang Diberi Perlakuan Kalsium Klorida Dan Asam Salisilat Yang Diadaptasikan Di Dataran Medium Jatinangor, Fiky Yulianto Wicaksono, Tati Nurmala, Sumadi

Jurnal Kultivasi

The objective of the research was to determine maximum yield of wheat that wereplanted on medium land which were treated calcium chloride and salicylic acid with an optimum concentration. The experiment was conducted from March untilJuly 2011 at The Experimental Station of Faculty of Agriculture, University of Padjadjaran, Jatinangor with an altitude of about 750 metres above sea level. The experimental design used was Randomized Block Design with factorial treatment design which consisted of two factors and replicated three times. The first factor was concentrations of calcium chloride which consisted of three levels: 0 M, 10-2 M, and 2 x …

Respon Tanaman Kelapa Sawit Terhadap Bakteri Pelarut Fosfat Dan Bakteri Penambat Nitrogen Di Pembibitan Awal (Pre Nursery), Intan Ratna Dewi Anjarsari, Mochamad Arief Soleh, Erni Suminar

Jurnal Kultivasi

The objective of this experiment was to study response of oil palm seedlings in prenursery to Phosfat solubilizing bacteria and nitogen fixaxing bacteria. This experiment was conducted at experimental field, Jatinangor, KabupatenSumedang from April until October 2012. Experimental field located at altitude 753 m above sea level with rainfall C type according to the classification of Schmidt and Fergusson. Experimental design was the simple pattern of Randomized Design Group (RDG), consisting of twelve treatment and repeated three times. The treatment were Without inoculation; Azotobacter 20 mL/plant; BPF 20 mL/plant; 10 mL/plant BPF + 10 ml/plant Azotobacter; 10mL/plant BPF + 15 …

Whole-Transciptome Analysis Of [Psi+] Budding Yeast Via Cdna Microarrays, Jared Carter

Theses

Introduction: Prions of yeast present a novel analytical challenge in terms of both initial characterization and in vitro manipulation as models for human disease research. Presently, few robust analysis strategies have been successfully implemented which enable the efficient study of prion behavior in vivo. This study sought to evaluate the utilization of conventional dual-channel cDNA microarrays for the surveillance of transcriptomic regulation patterns by the [PSI+] yeast prion relative to an identical prion deficient yeast variant, [psi-]. Methods: A data analysis and normalization workflow strategy was developed and applied to cDNA array images, yielded quality-regulated expression ratios for a subset …

Socio-Demographic Factors As Determinants Of Access And Use Of Ict By Staff Of University Libraries In Oyo State, Emmanuel Sola Owolabi

Library Philosophy and Practice (e-journal)

This study examined the influence of socio-demographic factors on access and use of ICT by staff of academic libraries in Oyo state. These universities are: Ladoke Akintola University, Lead City University and Bishop Ajayi Crowther.

Data was collected using a self structured questionnaire using convenience sampling technique. The empirical evidence was based on descriptive and statistical analysis using SPSS. The findings revealed that ease of use, perceived benefits and accessibility were highly correlated with the demographic factors (age, level of education and income), however, none of the demographic variables predicted favorably on challenges of ICT usage.

The study recommends that …

Glaucoma Research: A Scientometric Study Of Indian Publications Output, 2002-11, Brij Mohan Gupta, Adarsh Bala

Library Philosophy and Practice (e-journal)

Objectives: Analyses the Indian publications output in glaucoma research during 2002-11 on several parameters including contribution & citation impact of top most productive countries, India’s overall contribution, its growth pattern and citation impact, the share of international collaboration in India’s overall research output, contribution of leading countries and identification of leading foreign collaborating partners, Indian contribution & impact of different types of glaucoma, glaucoma research by sub-fields and glaucoma research output by different population age groups, productivity and impact of leading Indian institutions and authors and pattern of communication of Indian output in most productive journals.

Methods: The Scopus Citation …

Minutes, 2013 August 7

Agendas and Minutes

No abstract provided.

Agenda And Attachments, 2013 August 7

Agendas and Minutes

No abstract provided.

Computational And Biochemical Approaches To Molecular Epidemiology, Michael Calhoun, Hannah Dysinger, David Hobbs, Ph.D., Katie Hailer, Ph.D.

2013 Undergraduate Research

Mining activity in Butte, Montana has taken place, or continues to take place, within the urban residence of Butte itself. This has led to urban areas with high concentrations of toxic metals such as arsenic, lead, copper, zinc, mercury and cadmium. Advances in protein study and gene sequencing has opened the possibility of finding molecular biomarkers whose presence, absence or morphological changes could indicate disease processes in populations exposed to environmental toxins. While in principle, biomarkers can be any chemicals or metabolites, as well as proteins and genes that are indicative of exposure to xenobiotics, this study seeks to identify …