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2013

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Characterization Of A Stinging Nettle Composite As A Potential Battery Component, Sarah Johnson, Jeanne Franz Sep 2013

Characterization Of A Stinging Nettle Composite As A Potential Battery Component, Sarah Johnson, Jeanne Franz

Student Research and Creative Projects 2013-2014

The membrane in a galvanic cell was created with a composite made out of stinging nettle fibers and polypyrole. It was characterized with Thermo-gravimetric analysis (TGA), scanning electron microscopy (SEM), and a Potentiostat (CV). The TGA data showed that the composite was effectively created, the SEM data showed that the polypyrole did evenly coat the fiber after a change in procedure, and the CV data showed that the composite could potentially allow the flow of electrons. These facts all together show that the composite has the physical characteristics that were wanted and that the electrochemical characteristics need to be looked …


The Effects Of Connectivity On Fish Size And Community Structure In The Upper Mississippi River, Lucas Landry, Michael Delong Sep 2013

The Effects Of Connectivity On Fish Size And Community Structure In The Upper Mississippi River, Lucas Landry, Michael Delong

Student Research and Creative Projects 2013-2014

No abstract provided.


Phenyl Radical Cleavage From Tetraphenylphosphoranyl Radicals, Alex Larrabee, Thomas Nalli Sep 2013

Phenyl Radical Cleavage From Tetraphenylphosphoranyl Radicals, Alex Larrabee, Thomas Nalli

Student Research and Creative Projects 2013-2014

No abstract provided.


Macrophage Extracellular Trap Formation In Response To Mannheimia Haemolytica Or Its Leukotoxin Is Altered By Incubation With Conditioned Media From Bhv-1 Infected Bronchiolar Epithelial Cells, Haley Korth, Nicole Aulik Sep 2013

Macrophage Extracellular Trap Formation In Response To Mannheimia Haemolytica Or Its Leukotoxin Is Altered By Incubation With Conditioned Media From Bhv-1 Infected Bronchiolar Epithelial Cells, Haley Korth, Nicole Aulik

Student Research and Creative Projects 2013-2014

Bovine respiratory disease (BRD) is a serious lower respiratory tract infection characterized by inflammation, intense neutrophil infiltration, fibrin disposition, and consolidation of the lungs. BRD, also known as shipping fever, is the primary cause of morbidity in the U.S. beef and dairy industries. It is estimated that North American loses between 600 million and 3 billion dollars per year leading to a major concern for producers. BRD is caused by various viral and bacterial pathogens including Mannheimia haemolytica and Histophilus somni. M. haemolytica, a normal nasal inhabitant, is an important bacterial agent that causes BRD. The most important virulence factor …


Enzymatic Effects Of Pancreatic Amylase Inhibition By Kombucha Tea, Mallory Villeneuve, Francis Mann Sep 2013

Enzymatic Effects Of Pancreatic Amylase Inhibition By Kombucha Tea, Mallory Villeneuve, Francis Mann

Student Research and Creative Projects 2013-2014

Kombucha is a fermented tea beverage produced through use of a symbiotic culture of bacteria and yeast (SCOBY). The purported health benefits of this drink are being assessed through the use of an assay simulating breakdown of starches in the mouth.1 Analysis with spectrophotometry at 540 nm for porcine pancreas amylase using 0.100 mg/mL reported a Km of 0.440 mg/mL and Vmax of 0.00451 mg/min. Breakdown of starches was compared to a standard using maltose for comparison.2 Kombucha inhibition using IC50 inhibition analysis with a dose response curve of dilutions determined a value of 6.70x10-3 mL. Further IC50 analysis was …


Synthesis Of A Dye/Chitosan Complex For The Purification Of Lactate Dehydrogenase Via Column Chromatography, Kenneth Uy, Jennifer Zemke Sep 2013

Synthesis Of A Dye/Chitosan Complex For The Purification Of Lactate Dehydrogenase Via Column Chromatography, Kenneth Uy, Jennifer Zemke

Student Research and Creative Projects 2013-2014

This project strives to synthesize a novel dye/chitosan complex that will readily purify lactate dehydrogenase (LDH) from a homogenized chicken breast solution via affinity column chromatography. In doing so, a fiscally efficient alternative to Cibacron Blue 3-GA (CB3), a highly effective dye complex, will be elucidated. This project arose due to CB3’s price and absence from the market. Choosing an appropriate dye required the use of a molecular docking server to calculate the free binding energies (FBE) of various dyes to LDH. It was concluded that Eriochrome Black T (EBT) would most efficiently bind to LDH’s receptor site. This was …


Winona Currents Magazine, University Advancement - Winona State University Sep 2013

Winona Currents Magazine, University Advancement - Winona State University

Winona Currents

This magazine published by Winona State University’s University Advancement was intended to inform WSU Alumni, students, faculty, staff and the greater community about Alumni and the university.The cover theme is: Paying it Forward: Nicole Hinzie and other WSU Alums are giving back.


Metal-Assisted Etching Of Silicon Molds For Electroforming, Ralu Divan, Dan Rosenthal '14, Karim Ogando, Leonidas E. Ocola, Daniel Rosenmann, Nicolaie Moldovan Sep 2013

Metal-Assisted Etching Of Silicon Molds For Electroforming, Ralu Divan, Dan Rosenthal '14, Karim Ogando, Leonidas E. Ocola, Daniel Rosenmann, Nicolaie Moldovan

Student Publications & Research

Ordered arrays of high-aspect-ratio micro/nanostructures in semiconductors stirred a huge scientific interest due to their unique one-dimensional physical morphology and the associated electrical, mechanical, chemical, optoelectronic, and thermal properties. Metal-assisted chemical etching enables fabrication of such high aspect ratio Si nanostructures with controlled diameter, shape, length, and packing density, but suffers from structure deformation and shape inconsistency due to uncontrolled migration of noble metal structures during etching. Hereby the authors prove that a Ti adhesion layer helps in stabilizing gold structures, preventing their migration on the wafer surface while not impeding the etching. Based on this finding, the authors demonstrate …


Deceit And Transparency In Placebo Research, Stewart Justman Sep 2013

Deceit And Transparency In Placebo Research, Stewart Justman

Global Humanities and Religions Faculty Publications

Studies designed to elicit the full strength of the placebo effect differ from those in which the placebo effect represents a nuisance factor to be accounted for in order to establish the efficacy of a treatment. In the latter, informed consent is the rule; in the first, while consent may be informed in some narrow sense of the word, deception is common. However, the trickery of placebo experimentation goes beyond straightforward lies to include the use of crafty ambiguities, half-truths, and deliberate omissions in scripts read to the subjects of these studies. As words come to resemble therapeutic agents in …


Ntid Focus Fall 2013, Ntid Marketing And Communications Sep 2013

Ntid Focus Fall 2013, Ntid Marketing And Communications

National Technical Institute for the Deaf Papers

No abstract provided.


2012 Annual Report, Joseph A. Cook Sep 2013

2012 Annual Report, Joseph A. Cook

Annual Reports

No abstract provided.


Psyx 120.50: Introduction To Research Methods - Online, Brandon Rennie Sep 2013

Psyx 120.50: Introduction To Research Methods - Online, Brandon Rennie

University of Montana Course Syllabi

No abstract provided.


Biob 101n.02c: Discover Biology, Gregory Peters Sep 2013

Biob 101n.02c: Discover Biology, Gregory Peters

University of Montana Course Syllabi

No abstract provided.


A Pre-Zygotic Barrier To Hybridization In Two Con-Generic Species Of Scleractinian Corals, Andrew H. Baird, Vivian R. Cumbo, Joana Figueiredo, Saki Harii Sep 2013

A Pre-Zygotic Barrier To Hybridization In Two Con-Generic Species Of Scleractinian Corals, Andrew H. Baird, Vivian R. Cumbo, Joana Figueiredo, Saki Harii

Marine & Environmental Sciences Faculty Articles

Hybridization is often cited as a potential source of evolutionary novelty in the order Scleractinia. While hybrid embryos can be produced in vitro, it has been difficult to identify adult hybrids in the wild. Here, we tested the potential for hybridization between two closely related species in the family Fungiidae. We mixed approximately 5000 eggs of Ctenactis echinata with sperm from C. crassa. No hybrid embryos were produced. This observation adds to a growing body of evidence for pre-zygotic barriers to hybridization in corals and challenges the claim that hybridization is a major source of evolutionary novelty …


Ahst 200.01: Operating Room Techniques, Debbie S. Fillmore Sep 2013

Ahst 200.01: Operating Room Techniques, Debbie S. Fillmore

University of Montana Course Syllabi

No abstract provided.


Biob 191.01c: Discover Biology Teaching Assistant, Gregory Peters Sep 2013

Biob 191.01c: Discover Biology Teaching Assistant, Gregory Peters

University of Montana Course Syllabi

No abstract provided.


Anty 101h.01c Anthropology And The Human Experience, Rebecca J. Wood Sep 2013

Anty 101h.01c Anthropology And The Human Experience, Rebecca J. Wood

University of Montana Course Syllabi

No abstract provided.


Toward An Understanding Of The Retinal Chromophore In Rhodopsin Mimics, Mark M. Huntress, Samer Gozem, Konstantin R. Malley, Askat E. Jailaubekov, Chrysoula Vasileiou, Mikas Vengris, James H. Geiger, Babak Borhan, Igor Schapiro, Delmar S. Larsen, Massimo Olivucci Sep 2013

Toward An Understanding Of The Retinal Chromophore In Rhodopsin Mimics, Mark M. Huntress, Samer Gozem, Konstantin R. Malley, Askat E. Jailaubekov, Chrysoula Vasileiou, Mikas Vengris, James H. Geiger, Babak Borhan, Igor Schapiro, Delmar S. Larsen, Massimo Olivucci

Chemistry Faculty Publications

Recently, a rhodopsin protein mimic was constructed by combining mutants of the cellular retinoic acid binding protein II (CRABPII) with an all-trans retinal chromophore. Here, we present a combine computational quantum mechanics/molecular mechanics (QM/MM) and experimental ultrafast kinetic study of CRABPII. We employ the QM/MM models to study the absorption (lambda(a)(max)), fluorescence (lambda(f)(max)), and reactivity of a CRABPII triple mutant incorporating the all-trans protonated chromophore (PSB-KLE-CRABPII). We also study the spectroscopy of the same mutant incorporating the unprotonated chromophore and of another double mutant incorporating the neutral unbound retinal molecule held inside the pocket. Finally, for PSB-KLE-CRABPII, stationary fluorescence spectroscopy …


Bone: An Acute Buffer Of Plasma Sodium During Exhaustive Exercise?, Tamara Hew-Butler, Kristin J. Stuempfle, Martin D. Hoffman Sep 2013

Bone: An Acute Buffer Of Plasma Sodium During Exhaustive Exercise?, Tamara Hew-Butler, Kristin J. Stuempfle, Martin D. Hoffman

Health Sciences Faculty Publications

Both hyponatremia and osteopenia separately have been well documented in endurance athletes. Although bone has been shown to act as a “sodium reservoir” to buffer severe plasma sodium derangements in animals, recent data have suggested a similar function in humans. We aimed to explore if acute changes in bone mineral content were associated with changes in plasma sodium concentration in runners participating in a 161 km mountain footrace. Eighteen runners were recruited. Runners were tested immediately pre- and post-race for the following main outcome measures: bone mineral content (BMC) and density (BMD) via dual-energy X-ray absorptiometry (DEXA); plasma sodium concentration …


The Importance Of Dissimilatory Nitrate Reduction To Ammonium (Dnra) In The Nitrogen Cycle Of Coastal Ecosystems, Anne E. Giblin, Craig R. Tobias, Bongkeun Song, Nathaniel Weston, Gary T. Banta, Victor H. Rivera-Monroy Sep 2013

The Importance Of Dissimilatory Nitrate Reduction To Ammonium (Dnra) In The Nitrogen Cycle Of Coastal Ecosystems, Anne E. Giblin, Craig R. Tobias, Bongkeun Song, Nathaniel Weston, Gary T. Banta, Victor H. Rivera-Monroy

Faculty Publications

Until recently, it was believed that biological assimilation and gaseous nitrogen (N) loss through denitrification were the two major fates of nitrate entering or produced within most coastal ecosystems. Denitrification is often viewed as an important ecosystem service that removes reactive N from the ecosystem. However, there is a competing nitrate reduction process, dissimilatory nitrate reduction to ammonium (DNRA), that conserves N within the ecosystem. The recent application of nitrogen stable isotopes as tracers has generated growing evidence that DNRA is a major nitrogen pathway that cannot be ignored. Measurements comparing the importance of denitrification vs. DNRA in 55 coastal …


Integrated Carbon Budget Models For The Everglades Terrestrial-Coastal-Oceanic Gradient: Current Status And Needs For Inter-Site Comparisons, Tiffany G. Troxler, Evelyn Gaiser, Jordan Barr, Jose D. Fuentes, Rudolf Jaffé, Daniel L. Childers, Ligia Colado-Vides, Victor H. Rivera-Monroy, Edward Castañeda-Moya, William Anderson, Randy Chambers, Meilian Chen, Carlos Coronado-Molina, Stephen E. Davis, Victor Engel, Carl Fitz, James Fourqurean, Tom Frankovich, John Kominoski, Chris Madden, Sparkle L. Malone, Steve F. Oberbauer, Paulo Olivas, Jennifer Richards, Colin Saunders, Jessica Schedlbauer, Leonard J. Scinto, Fred Sklar, Tom Smith, Joseph M. Smoak, Gregory Starr, Robert R. Twilley, Kevin Whelan Sep 2013

Integrated Carbon Budget Models For The Everglades Terrestrial-Coastal-Oceanic Gradient: Current Status And Needs For Inter-Site Comparisons, Tiffany G. Troxler, Evelyn Gaiser, Jordan Barr, Jose D. Fuentes, Rudolf Jaffé, Daniel L. Childers, Ligia Colado-Vides, Victor H. Rivera-Monroy, Edward Castañeda-Moya, William Anderson, Randy Chambers, Meilian Chen, Carlos Coronado-Molina, Stephen E. Davis, Victor Engel, Carl Fitz, James Fourqurean, Tom Frankovich, John Kominoski, Chris Madden, Sparkle L. Malone, Steve F. Oberbauer, Paulo Olivas, Jennifer Richards, Colin Saunders, Jessica Schedlbauer, Leonard J. Scinto, Fred Sklar, Tom Smith, Joseph M. Smoak, Gregory Starr, Robert R. Twilley, Kevin Whelan

Faculty Publications

Recent studies suggest that coastal ecosystems can bury significantly more C than tropical forests, indicating that continued coastal development and exposure to sea level rise and storms will have global biogeochemical consequences. The Florida Coastal Everglades Long Term Ecological Research (FCE LTER) site provides an excellent subtropical system for examining carbon (C) balance because of its exposure to historical changes in freshwater distribution and sea level rise and its history of significant long-term carbon-cycling studies. FCE LTER scientists used net ecosystem C balance and net ecosystem exchange data to estimate C budgets for riverine mangrove, freshwater marsh, and seagrass meadows, …


Organic Carbon In Shallow Subterranean Habitats, Tanja Pipan, David C. Culver Sep 2013

Organic Carbon In Shallow Subterranean Habitats, Tanja Pipan, David C. Culver

KIP Articles

Organic carbon is likely to be a limiting factor in shallow subterranean habitats (SSHs). Data on dissolved organic carbon (DOC) in three SSHs are reviewed: (1) hypotelminorheic and associated seepage springs (Nanos Mountain, Slovenia), (2) hyporheic zones (Rhône River, France and seepage streams on Nanos Mountain, Slovenia), and (3) epikarst (China, Slovenia, and USA). Hypotelminorheic habitats are superficial groundwater sites less than 1 m below the surface that exit from seepage springs. Hyporheic habitats are the underflow of streams and rivers. Epikarst is the uppermost zone of karst with extensive small cavities and channels. Nanos hypotelminorheic sites that harbored stygobiotic …


Unh: The Magazine Of The University Of New Hampshire, Fall 2013, Unh Magazine Sep 2013

Unh: The Magazine Of The University Of New Hampshire, Fall 2013, Unh Magazine

UNH Magazine

No abstract provided.


Affect Of Cold Pressor Stress On Glycemic Response Of Healthy College Aged Subjects, Taylor Curtis, Samantha Krause, Katelyn Schmit, Frances Ragsdale Sep 2013

Affect Of Cold Pressor Stress On Glycemic Response Of Healthy College Aged Subjects, Taylor Curtis, Samantha Krause, Katelyn Schmit, Frances Ragsdale

Student Research and Creative Projects 2013-2014

Stress is associated with type 2 diabetes and acute stress could alter glycemic response. Physiological effects of cold presser stress (CPS: n=40) and control (no CPS; n=43) on g!ycemic and cardiovascular response to a 50 gram oral glucose tolerance test drink (OGTT) was examined in healthy humans (18±10 years). CPS consisted of three 30-second ice bath immersions of the left arm 10 min pre-OGTT (0 min). then 10 and 20 minutes post-OGTT. Blood glucose for control and CPS at -15. 0, 30, 60, 120 min was 89. 7±9. 0. 91. 0±8. 7, 154. 2±21. 5, 138. 8±32. 7, 96. 4±19. …


Macrophage Extracellular Trap Formation In Response To M. Haemolytica Or Its Lkt Is Altered By Co-Incubation With Bovine Herpes Virus-1 Infected Bronchiolar Epithelial Cells, Melissa Daley Sep 2013

Macrophage Extracellular Trap Formation In Response To M. Haemolytica Or Its Lkt Is Altered By Co-Incubation With Bovine Herpes Virus-1 Infected Bronchiolar Epithelial Cells, Melissa Daley

Student Research and Creative Projects 2013-2014

Bovine Respiratory Disease (BRD) is the primary cause of morbidity in the U.S. beef and dairy industries. BRD is caused by viral and bacterial agents that lead to severe pleuropneumonia in cattle, which is characterized by inflammation, intense neutrophil infiltration, consolidation and recently, extensive amounts of extracellular DNA in the lungs. One possible source of this DNA is from leukocytes that release fibrillar network referred to as extracellular traps (ETs). Previously, it was demonstrated that neutrophils and macrophages produce ETs in response to Mannheimia haemolytica and its leukotoxin (LKT). Conditioned media removed from bovine herpes virus (BHV)-infected bovine bronchiole epithelial …


Identification Of Carriers Of Babesia Microti From Local Populations Of Borrelia-Infested Ticks, Terra Fodstad Sep 2013

Identification Of Carriers Of Babesia Microti From Local Populations Of Borrelia-Infested Ticks, Terra Fodstad

Student Research and Creative Projects 2013-2014

Babesia microti is the causative agent for babesiosis, a blood-based parasitic disease that causes malaria-like symptoms in humans. It is passed through Ixodes scapularis (deer ticks) and is rising in incidence in the upper Midwest. Ticks that carry Borrelia, the causative agent for Lyme disease, may also carry Babesia microti. There are two major goals in this project: determine the prevalence of Babesia microti in ticks in the Winona area and determine whether those ticks that carry Borrelia are more likely to carry Babesia microti. The first part of the project requires development of a PCR assay that works well …


The Influence Of Gross Primary Production On Trophic Complexity In Slackwaters Of Large Rivers, Mckenzie Kline, Michael Delong Sep 2013

The Influence Of Gross Primary Production On Trophic Complexity In Slackwaters Of Large Rivers, Mckenzie Kline, Michael Delong

Student Research and Creative Projects 2013-2014

No abstract provided.


Isolation And Characterization Of Microbial Pathogens Found In Chickens, Kurt Lawton, John Weber Sep 2013

Isolation And Characterization Of Microbial Pathogens Found In Chickens, Kurt Lawton, John Weber

Student Research and Creative Projects 2013-2014

The bacterial content of retail chicken is a topic of growing concern. Chicken processing plants are under increasingly strict regulations to prevent contamination. Likewise, increasing controversy is being directed towards chicken farmers concerning cleanliness and the use of antibiotics in raising chickens, as it may lead to antibiotic resistance. There is legitimate concern surrounding the many possible pathogens associated with chicken, let alone the recent trend of bacteria with heightened resistance to antibiotics. This project was carried out with the purpose of the identification and characterization of bacteria found in chicken from local supermarkets. As multiple bacterial species were expected …


Hemoglobin Sequences Of The Painted Turtle, Erin Liepold, Audra Harvey, Amy Runck Sep 2013

Hemoglobin Sequences Of The Painted Turtle, Erin Liepold, Audra Harvey, Amy Runck

Student Research and Creative Projects 2013-2014

Through evolution, hemoglobin oxygen affinity has been altered in species that live in different environments. Hemoglobin is the blood protein that carries O2 from the lungs to the tissues, and returns CO2 (from the tissues) to the lungs. Members of the family Testudinidae (turtles and tortoises) live under different environmental conditions and thus experience different oxygen levels. The hemoglobin of adult turtles is comprised of α- globin (alpha-D and alpha-A) and β-globin. For this research, hemoglobin of the terrestrial African spurred tortoise (Geochelone sulcata) and the aquatic painted turtle (Chrysemys picta) were analyzed. The African spurred tortoise lives in the …


Functional Analyses Of Tiger Salamander (Ambystoma Tigrinum) Hemoglobin, Jorge Polanco, Erica Eischens, Cody Benedict, Gabriel Velez Sep 2013

Functional Analyses Of Tiger Salamander (Ambystoma Tigrinum) Hemoglobin, Jorge Polanco, Erica Eischens, Cody Benedict, Gabriel Velez

Student Research and Creative Projects 2013-2014

No abstract provided.