Digital Commons Network^™

Investigating "Experimentalism" : A Case Study Of The Tuba And Its Repertoire, Andrew Brian Larson

LSU Doctoral Dissertations

The extant repertoire for the tuba serves as a landmark for how the tuba was perceived at that moment in time by that composer. This document contains a brief analysis of the tuba “experiment” that has been ongoing since its invention. In addition, it contains a brief parallel case study of the saxophone and how this instrument, invented at about the same time as the tuba, has embraced experimentalism and modern performance. This document contains five major sections. The first provides a brief history of the tuba and its predecessors. The second introduces numerical data representing the performance frequency of …

Amplifying Conjugated Polymers For Fluorescent Chemosensing, Deepa Pangeni

LSU Doctoral Dissertations

Chemo- and biosensors based on fluorescent conjugated polymers benefit from greater detection sensitivity due to amplification of the electronic perturbations produced by analyte binding. This amplification stems from the exciton-transporting properties of conjugated polymers. In the conventional sensor design paradigm, excitons migrate from the bulk of the polymer to the analyte binding sites which can be either fluorescence quenching sites (turn-off sensors) or lower energy fluorophores (turn-on sensors). In this dissertation, we proposed an alternative design paradigm when analyte binding creates a higher energy gap site in the polymer backbone. In the case of isolated polymer chains in dilute solution, …

Medicinal Plants Of Northern Thailand For The Treatment Of Cognitive Impairment In The Elderly, Lisa C. Offringa

Dissertations, Theses, and Capstone Projects

Dementia is a progressive neurological disease affecting memory and behavior. The diagnosis of dementia is increasing exponentially worldwide and with it the potential risk for a severe social and economic burden of caring for an increasing debilitated elderly population. Cognitive impairment, and especially memory loss, can be the first indication of dementia. This study documents the treatment of cognitive impairment in the elderly by Thai traditional healers in northern Thailand usingmedicinal plants. Interviews were conducted from 2008-2012 to investigate the etiology of dementia in Thai Traditional Medicine and identify plants used to treat memory loss in the pharmacopeia of northern …

Ash2 Acts As An Ecdysone Receptor Coactivator By Stabilizing The Histone Methyltransferase Trr., Albert Carbonell, Alexander Mazo, Florenci Serras, Montserrat Corominas

Department of Biochemistry and Molecular Biology Faculty Papers

The molting hormone ecdysone triggers chromatin changes via histone modifications that are important for gene regulation. On hormone activation, the ecdysone receptor (EcR) binds to the SET domain-containing histone H3 methyltransferase trithorax-related protein (Trr). Methylation of histone H3 at lysine 4 (H3K4me), which is associated with transcriptional activation, requires several cofactors, including Ash2. We find that ash2 mutants have severe defects in pupariation and metamorphosis due to a lack of activation of ecdysone-responsive genes. This transcriptional defect is caused by the absence of the H3K4me3 marks set by Trr in these genes. We present evidence that Ash2 interacts with Trr …

Molecular Determinants Of Epidermal Growth Factor Binding: A Molecular Dynamics Study., Jeffrey M Sanders, Matthew E Wampole, Mathew L. Thakur, Eric Wickstrom

Department of Biochemistry and Molecular Biology Faculty Papers

The epidermal growth factor receptor (EGFR) is a member of the receptor tyrosine kinase family that plays a role in multiple cellular processes. Activation of EGFR requires binding of a ligand on the extracellular domain to promote conformational changes leading to dimerization and transphosphorylation of intracellular kinase domains. Seven ligands are known to bind EGFR with affinities ranging from sub-nanomolar to near micromolar dissociation constants. In the case of EGFR, distinct conformational states assumed upon binding a ligand is thought to be a determining factor in activation of a downstream signaling network. Previous biochemical studies suggest the existence of both …

The Tip Of The Tail Needle Affects The Rate Of Dna Delivery By Bacteriophage P22., Justin C Leavitt, Lasha Gogokhia, Eddie B Gilcrease, Anshul Bhardwaj, Gino Cingolani, Sherwood R Casjens

Department of Biochemistry and Molecular Biology Faculty Papers

The P22-like bacteriophages have short tails. Their virions bind to their polysaccharide receptors through six trimeric tailspike proteins that surround the tail tip. These short tails also have a trimeric needle protein that extends beyond the tailspikes from the center of the tail tip, in a position that suggests that it should make first contact with the host's outer membrane during the infection process. The base of the needle serves as a plug that keeps the DNA in the virion, but role of the needle during adsorption and DNA injection is not well understood. Among the P22-like phages are needle …

Structural Implications For Selective Targeting Of Parps., Jamin D Steffen, Md, Jonathan Brody, Md, Roger S Armen, Md, John M Pascal, Md

Department of Biochemistry and Molecular Biology Faculty Papers

Poly(ADP-ribose) polymerases (PARPs) are a family of enzymes that use NAD(+) as a substrate to synthesize polymers of ADP-ribose (PAR) as post-translational modifications of proteins. PARPs have important cellular roles that include preserving genomic integrity, telomere maintenance, transcriptional regulation, and cell fate determination. The diverse biological roles of PARPs have made them attractive therapeutic targets, which have fueled the pursuit of small molecule PARP inhibitors. The design of PARP inhibitors has matured over the past several years resulting in several lead candidates in clinical trials. PARP inhibitors are mainly used in clinical trials to treat cancer, particularly as sensitizing agents …

Identification Of Phosphorylation Sites In The Cooh-Terminal Tail Of The Μ-Opioid Receptor., Ying-Ju Chen, Sue Oldfield, Adrian J. Butcher, Andrew B. Tobin, Kunal Saxena, Vsevolod V. Gurevich, Jeffrey L. Benovic, Graeme Henderson, Eamonn Kelly

Department of Biochemistry and Molecular Biology Faculty Papers

Phosphorylation is considered a key event in the signalling and regulation of the μ opioid receptor (MOPr). Here, we used mass spectroscopy to determine the phosphorylation status of the C-terminal tail of the rat MOPr expressed in human embryonic kidney 293 (HEK-293) cells. Under basal conditions, MOPr is phosphorylated on Ser(363) and Thr(370), while in the presence of morphine or [D-Ala2, NMe-Phe4, Gly-ol5]-enkephalin (DAMGO), the COOH terminus is phosphorylated at three additional residues, Ser(356) , Thr(357) and Ser(375). Using N-terminal glutathione S transferase (GST) fusion proteins of the cytoplasmic, C-terminal tail of MOPr and point mutations of the same, we …

Gender And New Wars, Christine M. Chinkin, Mary Kaldor

Articles

War plays an important role in the construction of gender, or the social roles of men and women. This article analyzes the gendered experience of what Kaldor calls "new wars." It shows that new wars are largely fought by men in the name of a political identity that usually has a significant gender dimension. They use tactics that involve deliberate attacks on civilians, including systematic rape as a weapon of war, and are financed by predatory economic activities that tend to affect women more than men. The article describes the ways in which laws relating to gendered violence have been …

Predicting Alzheimer Disease With Β-Amyloid Imaging: Results From The Australian Imaging, Biomarkers, And Lifestyle Study Of Ageing, Christopher Rowe, Pierrick Bourgeat, Kathryn Ellis, Belinda M. Brown, Lim Yen Ying, Rachel Mulligan, Gareth Jones, Paul Maruff, Michael Woodward, Roger Price, Peter Robins, Henri Tochon-Danguy, Graeme O'Keefe, Kerryn Pike, Paul Yates, Cassandra Szoeke, Olivier Salvado, S Lance Macaulay, Tim O'Meara, Richard Head, Lynne Cobiac, Greg Savage, Ralph N. Martins, Colin Masters, David Ames, Victor Villemagne

Research outputs 2013

Objective: Biomarkers for Alzheimer disease (AD) can detect the disease pathology in asymptomatic subjects and individuals with mild cognitive impairment (MCI), but their cognitive prognosis remains uncertain. We aimed to determine the prognostic value of β-amyloid imaging, alone and in combination with memory performance, hippocampal atrophy, and apolipoprotein E ε4 status in nondemented, older individuals. Methods: A total of 183 healthy individuals (age = 72.0 ± 7.26 years) and 87 participants with MCI (age = 73.7 ± 8.27) in the Australian Imaging, Biomarkers, and Lifestyle study of ageing were studied. Clinical reclassification was performed after 3 years, blind to biomarker …

Smaller, Faster Stomata: Scaling Of Stomatal Size, Rate Of Response, And Stomatal Conductance, Paul Drake, Raymond H. Froend, Peter Franks

Research outputs 2013

Maximum and minimum stomatal conductance, as well as stomatal size and rate of response, are known to vary widely across plant species, but the functional relationship between these static and dynamic stomatal properties is unknown. The objective of this study was to test three hypotheses: (i) operating stomatal conductance under standard conditions (gop) correlates with minimum stomatal conductance prior to morning light [gmin(dawn)]; (ii) stomatal size (S) is negatively correlated with gop and the maximum rate of stomatal opening in response to light, (dg/dt)max; and (iii) gop correlates negatively with instantaneous water-use efficiency (WUE) despite positive correlations with maximum rate …

Children And Elephants: A Study Of Mentalization, Empathy, And Attitudes Towards Conservation In Participants Of An Elephant-Based Environmental Intervention In West Africa, Erica C. Rogers

Dissertations, Theses, and Capstone Projects

Recent research suggests that children's identification with animals and propensity to learn from animal experiences might make animal-focused interventions ideal for social and emotional development. A child's ability to understand their own and others' feeling states has been linked to emotional resilience and has been identified as a protective factor against the development of pathology later in life (Allen & Fonagy, 2006). This study examined the impact of an ongoing conservation-based intervention in Burkina Faso on conservation attitudes, mentalization and empathy. Participants were 106 Burkinabe students, 56 male and 50 female, ages 9-15. Participants were split into four groups, a …

Biodiesel Production From Candlenut And Calodendrum Capense Seeds: Process Design And Technological Assessment, Dennis Mogire Ndaya

All Master’s Theses

This project was based on biodiesel production from candlenut and calodendrum capense seeds using conventional and biotechnological technique and determination of the oil yields and biodiesel quality. The driving force for large-scale use of biodiesel has been the need to reduce the harmful emissions that result from the burning of petroleum oil as well as our dependence on diminishing reserves of petroleum oil. The aim of the study was to extract oil from Candlenut tree and Calodendrum capense seeds using conventional techniques and determine conditions for optimum oil yields. The oil from the two non-edible plant feedstocks was obtained using …

Characterization Of Flavonoids From Candidate Striga Weed Controlling Food Legumes; Cicer Arietinum L. And Vigna Radiata L., Anna Akumu Anyango

All Master’s Theses

In Africa, intercropping of some legumes with cereal crops has been found to cause remarkable reduction of Striga infestation and improved production of cereal crop. Desmodium uncinatum controls Striga infestation in intercrop with cereals through an allelopathic mechanism, which involves post-germination growth inhibitors exuded from the roots. Allelopathy is a natural and environmentally friendly technique, which has a potential to be a new approach for weed control. A standardized profiling method based on Reverse Phase-High Performance Liquid Chromatography (RP-HPLC) and Liquid Chromatography coupled with Mass Spectrometry (LC/MS) was used to identify flavonoids in extracts of chickpea (Cicer arietinum) and mung …

Current Approaches In Chemical Ecology Using Regionally Adapted Sustainable Methods, Eva Winter

All Master’s Theses

Kenya is an African developing country situated at the equator ranking 157th of 177 countries in the human development index. Agriculture is next to tourism the most important economic sector with 24 % of the GDP. Poverty prevalence is highest with small holder farmers. A promissing approach to reduce this precarious condition is the implementation of organic agriculture. 1. Establishment of certified organic cash crop production for a domestic and/or an export market and 2. dissemination of common methods in organic agriculture to subsistently living small holder farmers are the two major strategies of the approach. Both have different positive …

Homology Model Of 30s Ribosomal Subunit From Mycobacteria Tuberculosis, Peterson Gitonga Mathenge

All Master’s Theses

Mycobacterium tuberculosis, the causative agent of the tuberculosis, has infected more than a third of the world population to date. It has been known to be a very aggressive bacterium that is highly resistant to current drugs that target Tuberculosis. Antibiotics such as viomycin and capreomycin have been shown to bind to functionally important regions of the bacterial ribosome inhibiting protein synthesis process thereby affecting the bacterial cells viability. It is hypothesized that, a three dimensional structure of the 30S ribosomal subunit of the bacterium, will bring about a novel approach on drug target, and will be important in the …

Responses To Pyrethroids In Heavy Metal Tolerant Anopheles Gambiae Vector Of Malaria, Fauzia Khagai Musasia

All Master’s Theses

Anopheles gambiae mosquito, the principle Afro-tropical vector of malaria, has been documented in habitats with heavy metals in excess of natural loads. The mosquito has also displayed resistance to most conventional insecticides, and potential to resist challenges by heavy metals. Investigations were conducted in strains of An. gambiae ss, in which tolerance to cadmium and lead heavy metals were established. This study was undertaken to determine any putative relationship between tolerance to (cadmium and lead) heavy metals, and resistance to pyrethroid insecticide in An. gambiae. Tolerance to cadmium and lead heavy metals was developed in larval stages and this development …

Protective Role Of Antioxidant In Ameliorating African Trypanosomiasis - Induced Brain Degeneration In A Mouse Model, James Nyabuga Nyariki

All Master’s Theses

Late stage Human African Trypanosomiasis (HAT) is manifested by brain degeneration following infection by Trypanasoma brucei rhodensiense and Trypanasoma brucei gambiense parasites. This stage can only be treated with melarsoprol (Mel B) which inadvertently induces Post Treatment Reactive Encephalopathy (PTRE) and a mortality of 5% among HAT infected patients. This is an unacceptable mortality for a modern human drug. Investigations were conducted to establish the protective role of Coenzyme Q10 (CoQ10) and endogenous antioxidants (Manganese Superoxide dismutase (MnSOD), Glutathione Reductase (GR), Copper-Zinc Superoxide dismutase (SOD-1) and reduced glutathione (GSH)) against PTRE and putative resultant brain degeneration in a mouse model. …

Effect Of Temperature On The Efficacy Of Metarhizium Anisopliae (Metchnikoff) Sorokin In The Control Of Western Flower Thrips In French Beans, Maureen Nandwa Kuboka

All Master’s Theses

Metarhizium anisopliae isolate ICIPE69 is a highly virulent fungal pathogen against several thrips species such as western flower thrips (Frankliniella occidentalis), bean flower thrips (Megalurothrips sjostedti), onion thrips (Thrips tabaci) and others infesting key vegetable crops in Africa. Recently the isolate has been commercialized for pest control under the trade name, Campaign ®. The efficacy of the entomopathogenic fungi is known to be influenced by the prevailing climatic conditions with temperature being a key climate driver. This study aimed to predict the efficacy of Campaign® for thrips control in different Agro ecological zones (AEZ) in Kenya. To achieve this, effects …

Log Ratio Methodology For Analysis Of Compositional Data: A Case Of Olfactometer Bioassay Data From Insect Behavioural Studies, Anthony Raymond Epel

All Master’s Theses

Researchers are often times confronted with compositional data in insect choice studies. The choice of a statistical method to model this type of data is always not obvious. In this study, three approaches for analysis of compositional data from choice tests made by the predatory parasitoid Cotesia sesamaie Cameron in a four-arm olfactometer was explored using centered, additive and isometric log ratio transformations. Oviposition induced plant volatiles (OIPVs), herbivore induced plant volatiles (HIPVs), and two controls were tested in the four-arm olfactometer. The response variable measured was time spent in each field of the olfactometer, when the time to observe …

Assessment Of The Potential For Integration Of Ecosystem Based Approaches And Local Indigenous Knowledge Into Climate Change Adaptation In The Taita Hills, Kenya, Sarah Murabula Achola

All Master’s Theses

The integration of indigenous knowledge and science through and ecosystem based adaptation provides a basis for the formulation of culturally acceptable and sustainable adaptation practices. This study aims to determine sustainable climate change adaptation strategies for the Taita Hills in Kenya and assess their potential for integration with ecosystem based adaptation. It uses a mixed methodology that involves literature review, participatory methods and household surveys. As a result it emerges that adaptation to climate variability and change in the Taita Hills, takes on both an anticipatory and reactive approach. The household survey indicates that 68% of the farmers have taken …

Relative Sensitivity Of Two Marine Bivalves For Detection Of Genotoxic And Cytotoxic Effects: A Field Assessment In The Tamar Estuary, South West England., Lorna J. Dallas, Victoria V. Cheung, Andrew S. Fisher, Awadhesh N. Jha

School of Geography, Earth and Environmental Sciences

The input of anthropogenic contaminants to the aquatic environment is a major concern for scientists, regulators and the public. This is especially relevant in areas such as the Tamar valley in SW England, which has a legacy of contamination from industrial activity in the nineteenth and twentieth centuries. Following on from previous laboratory validation studies, this study aimed to assess the relationship between genotoxic and cytotoxic responses and heavy metal concentrations in two bivalve species sampled from locations along the Tamar estuary. Adult cockles, Cerastoderma edule, and blue mussels, Mytilus edulis, were sampled from five locations in the Tamar and …

Imagining A Same-Sex Marriage Decision Based On Dignity: Considering Human Experience In Constitutional Law, Danieli Evans

Articles

California’s Proposition 8 allows same sex couples to join through civil unions, which grant the legal benefits afforded to married couples but denies them the official label of “marriage.” The lower court eschewed the question of whether Proposition 8 burdens any fundamental right by concluding there is not even a rational basis (the minimum standard for the constitutionality of any law) for this law, as its sole effect is to deny same sex couples the designation of marriage.

We appreciate the lower court’s caution not to extend our fundamentalrights jurisprudence beyond precedent. However, we are positioned to elaborate fundamental constitutional …

Against Notice Skepticism In Privacy (And Elsewhere), M. Ryan Calo

Articles

What follows is an exploration of innovative new ways to deliver privacy notice. Unlike traditional notice that relies upon text or symbols to convey information, emerging strategies of “visceral” notice leverage a consumer’s very experience of a product or service to warn or inform. A regulation might require that a cell phone camera make a shutter sound so people know their photo is being taken. Or a law could incentivize websites to be more formal (as opposed to casual) wherever they collect personal information, as formality tends to place people on greater guard about what they disclose. The thesis of …

Only Part Of The Picture: A Response To Professor Tushnet's Worth A Thousand Words, Zahr Kassim Said

Articles

Professor Rebecca Tushnet’s recent article Worth a Thousand Words: The Image of Copyright elucidates a number of difficulties in copyright that flow from judicial failures to treat images consistently and rigorously. She argues that courts both assess copyrightability and evaluate potential infringement in ways that rely on a naïve understanding of the way artists create, and indeed, the way viewers receive works of art. The problem is particularly pronounced with respect to what Tushnet calls non-textual works because copyright law’s default to textuality means that the tools and methods that judges use misalign with the objects of their examination.

In …

Performance Evaluation Of Data Intensive Computing In The Cloud, Bhagavathi Kaza

UNF Graduate Theses and Dissertations

Big data is a topic of active research in the cloud community. With increasing demand for data storage in the cloud, study of data-intensive applications is becoming a primary focus. Data-intensive applications involve high CPU usage for processing large volumes of data on the scale of terabytes or petabytes. While some research exists for the performance effect of data intensive applications in the cloud, none of the research compares the Amazon Elastic Compute Cloud (Amazon EC2) and Google Compute Engine (GCE) clouds using multiple benchmarks. This study performs extensive research on the Amazon EC2 and GCE clouds using the TeraSort, …

Comparative Sugar Transport By Crustacean Hepatopancreas And Intestine, Ada Duka

UNF Graduate Theses and Dissertations

Glucose is transported in crustacean hepatopancreas and intestine by Na⁺-dependent co-transport, while Na⁺-dependent D-fructose influx has only been described for the hepatopancreas. It is still unclear if the two sugars are independently transported by two distinct cotransporter carrier systems. In this study lobster (Homarus americanus) hepatopancreas brush border membrane vesicles (BBMV) were used to characterize, in detail, the cation-dependency of both D-[³H] glucose and D-[³H] fructose influxes, while in vitro perfused intestines were employed to determine the nature of cation-dependent sugar transport in this organ. Over the sodium concentration range …

Classroom Intercultural Competence In Teacher Education Students, Interns, And Alumni, Christine K. Holland

UNF Graduate Theses and Dissertations

The purpose of this mixed methods study was to explore the impact of a student teaching internship upon the classroom intercultural competence (CIC) of teacher education students and alumni. Phase I employed quantitative methodology to compare the intercultural competence as measured by Ross, Thornson, McDonald, and Arrastia’s (2009) Cross Cultural Competence Inventory (3CI) for three groups. The 3CI is a 63-item survey that uses corresponding questions to assess intercultural competence. Survey items are scored with a 6-point, strongly agree-to-strongly disagree Likert scale (Thornson, 2010). The three groups included teacher education students enrolled in a field observation course, students enrolled in …

Under Siege: The U.S. Live Cattle Industry, Bill Bullard

South Dakota Law Review

No abstract provided.

Gene X Environment Effects Of Serotonin Transporter, Dopamine Receptor D4, And Monoamine Oxidase A Genes With Contextual And Parenting Risk Factors On Symptoms Of Oppositional Defiant Disorder, Anxiety, And Depression In A Community Sample Of 4-Year-Old Children, John V. Lavigne, Laura B.K. Herzing, Edwin H. Cook, Susan A. Lebailly, Karen R. Gouze, Joyce Hopkins, Fred B. Bryant

Psychology: Faculty Publications and Other Works

Genetic factors can play a role in the multiple level of analyses approach to understanding the development of child psychology. The present study examined gene-environment correlations and Gene x Environment interactions for polymorphisms of three target genes, the serotonin transporter gene, the D4 dopamine reactor gene, and the monoamine oxidase A gene in relation to symptoms of anxiety, depression, and oppositional behavior. Saliva samples were collected from 175 non-Hispanic White, 4-year-old children. Psychosocial risk factors included socioeconomic status, life stress, caretaker depression, parental support, hostility, and scaffolding skills. In comparison with the short forms (s/s, s/l) of the serotonin transporter …