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2013

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Mitochondrial Dna Instability In Cells Lacking Aconitase Correlates With Iron Citrate Toxicity, Zhengchang Liu, Fei Xiao, Zhejun Dong, Tammy Pracheil, Muhammad Farooq Jan 2013

Mitochondrial Dna Instability In Cells Lacking Aconitase Correlates With Iron Citrate Toxicity, Zhengchang Liu, Fei Xiao, Zhejun Dong, Tammy Pracheil, Muhammad Farooq

Biological Sciences Faculty Publications

Aconitase, the second enzyme of the tricarboxylic acid cycle encoded by ACO1 in the budding yeast Saccharomyces cerevisiae, catalyzes the conversion of citrate to isocitrate. aco1Δ results in mitochondrial DNA (mtDNA) instability. It has been proposed that Aco1 binds to mtDNA and mediates its maintenance. Here we propose an alternative mechanism to account for mtDNA loss in aco1Δ mutant cells. We found that aco1Δ activated the RTG pathway, resulting in increased expression of genes encoding citrate synthase. By deleting RTG1, RTG3, or genes encoding citrate synthase, mtDNA instability was prevented in aco1Δ mutant cells. Increased activity of citrate synthase leads …


Progress Towards Understanding Of Mechanisms Of Action Of Potent Multifunctional Disease Modifying Therapeutics For Parkinson's Disease & Investigating The Methamphetamine-Induced Striatal Microglia Activation., Mrudang M. Shah Jan 2013

Progress Towards Understanding Of Mechanisms Of Action Of Potent Multifunctional Disease Modifying Therapeutics For Parkinson's Disease & Investigating The Methamphetamine-Induced Striatal Microglia Activation., Mrudang M. Shah

Wayne State University Dissertations

PROGRESS TOWARDS UNDERSTANDING OF MECHANISMS OF ACTION OF POTENT MULTIFUNCTIONAL DISEASE MODIFYING

THERAPEUTICS FOR PARKINSON'S DISEASE.

by

MRUDANG MANOJKUMAR SHAH

December 2013

Advisor: Dr. Aloke Dutta

Major: Pharmaceutical Sciences

Degree: Doctor of Philosophy

Our long term goal is to design and develop potent multifunctional disease modifying therapeutics for Parkinson's disease. The objective of my dissertation was to understand the mechanisms of action of some potent small molecules (synthesized in our lab) as a disease modifying Parkinson's disease therapeutic. The objective was achieved by pursuing the following two specific aims:

1. Investigating anti-oxidant and neuroprotective effects of a lead molecule (D-512) …


The Traumatized/Traumatizing Subject In Anna Deavere Smith, Suzan-Lori Parks, And August Wilson, Christopher J. Giroux Jan 2013

The Traumatized/Traumatizing Subject In Anna Deavere Smith, Suzan-Lori Parks, And August Wilson, Christopher J. Giroux

Wayne State University Dissertations

The Traumatized/Traumatizing Subject in Anna Deavere Smith, Suzan-Lori Parks, and August Wilson explores how drama, as a genre, is particularly suited for capturing aspects of trauma in ways that other genres cannot; this argument reinforces that trauma must be studied within the humanities, specifically within literature programs. Recognizing that trauma in and of itself is tied to issues of narrativization, textuality, and performativity, this project notes that Smith's, Parks's, and Wilson's work collectively attempt to traumatize contemporary viewing audiences and make them aware—cognitively and emotionally—of the hardships of discrimination and racism, which themselves can be seen as examples of generational …


Scfslimb Ubiquitin Ligase Suppresses Condensin Ii–Mediated Nuclear Reorganization By Degrading Cap-H2, Daniel W. Buster, Scott G. Daniel, Huy Q. Nguyen, Sarah L. Windler, Lara C. Skwarek, Maureen Peterson Jan 2013

Scfslimb Ubiquitin Ligase Suppresses Condensin Ii–Mediated Nuclear Reorganization By Degrading Cap-H2, Daniel W. Buster, Scott G. Daniel, Huy Q. Nguyen, Sarah L. Windler, Lara C. Skwarek, Maureen Peterson

Dartmouth Scholarship

Condensin complexes play vital roles in chromosome condensation during mitosis and meiosis. Condensin II uniquely localizes to chromatin throughout the cell cycle and, in addition to its mitotic duties, modulates chromosome organization and gene expression during interphase. Mitotic condensin activity is regulated by phosphorylation, but mechanisms that regulate condensin II during interphase are unclear. Here, we report that condensin II is inactivated when its subunit Cap-H2 is targeted for degradation by the SCF(Slimb) ubiquitin ligase complex and that disruption of this process dramatically changed interphase chromatin organization. Inhibition of SCF(Slimb) function reorganized interphase chromosomes into dense, compact domains and disrupted …


Sci-Tech Book News Reviews, Susan Fingerman Jan 2013

Sci-Tech Book News Reviews, Susan Fingerman

Sci-Tech News

No abstract provided.


As Clear As Mud: A Critical Review Of Evidence For The Ecological Roles Of Australian Dingoes, Benjamin L.R. Allen, Peter J.S. Fleming, Lee R. Allen, Richard M. Engeman, Guy Ballard, Luke K.-P. Leung Jan 2013

As Clear As Mud: A Critical Review Of Evidence For The Ecological Roles Of Australian Dingoes, Benjamin L.R. Allen, Peter J.S. Fleming, Lee R. Allen, Richard M. Engeman, Guy Ballard, Luke K.-P. Leung

United States Department of Agriculture Wildlife Services: Staff Publications

Top-predators have been reported to have an important role in structuring food webs and maintaining ecological processes for the benefit of biodiversity at lower trophic levels. This is thought to be achieved through their suppressive effects on sympatric mesopredators and prey. Great scientific and public interest surrounds the potential use of top-predators as biodiversity conservation tools, and it can often be difficult to separate what we think we know and what we really know about their ecological utility. Not all the claims made about the ecological roles of top-predators can be substantiated by current evidence. We review the methodology underpinning …


Patterns Of Human–Coyote Conflicts In The Denver Metropolitan Area, Sharon A. Poessel, Stewart W. Breck, Tara L. Teel, Stephanie A. Shwiff, Kevin R. Crooks, Lisa Angeloni Jan 2013

Patterns Of Human–Coyote Conflicts In The Denver Metropolitan Area, Sharon A. Poessel, Stewart W. Breck, Tara L. Teel, Stephanie A. Shwiff, Kevin R. Crooks, Lisa Angeloni

United States Department of Agriculture Wildlife Services: Staff Publications

In many cities throughout North America, human–coyote conflicts are an emerging problem. Little research has described temporal and spatial patterns of human–coyote conflicts, although such information can be an important step in developing and optimizing management efforts. We used reports from 22 entities within the Denver Metropolitan Area (DMA) in Colorado that provided information on coyote observations (signs, sightings, and encounters) and conflicts (incidents, pet-attacks, and human attacks). Our objectives were to 1) define, quantify, and map categories of coyote observations and conflicts, and 2) investigate temporal and spatial patterns of conflicts, particularly related to land cover types and housing …


Influence Of Vegetation Structure On The Small Mammal Community In A Shortgrass Prairie Ecosystem, Craig M. Thompson, Eric M. Gese Jan 2013

Influence Of Vegetation Structure On The Small Mammal Community In A Shortgrass Prairie Ecosystem, Craig M. Thompson, Eric M. Gese

United States Department of Agriculture Wildlife Services: Staff Publications

The structure of vegetation, and how this structure varies across a landscape, is crucial to understanding the distribution of wildlife species. Between 2002 and 2004, we sampled small mammal communities and measured vegetation structure at 185 locations across a range of disturbance regimes in a shortgrass prairie ecosystem in southeastern Colorado, USA. At each sampling location, the local disturbance regime was some combination of varying intensity of livestock grazing, military training activity, and fire. Vegetation structural characteristics measured included percent bare ground, basal cover, litter, shrub density, and mean grass and shrub height. Rodent communities were described by richness, diversity, …


As Clear As Mud: A Critical Review Of Evidence For The Ecological Roles Of Australian Dingoes, Benjamin L. Allen, Peter J.S. Fleming, Lee R. Allen, Richard M. Engeman, Guy Ballard, Luke K.-P. Leung Jan 2013

As Clear As Mud: A Critical Review Of Evidence For The Ecological Roles Of Australian Dingoes, Benjamin L. Allen, Peter J.S. Fleming, Lee R. Allen, Richard M. Engeman, Guy Ballard, Luke K.-P. Leung

United States Department of Agriculture Wildlife Services: Staff Publications

Contents

1. Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . …


Nonsurgical Fertility Control For Managing Free-Roaming Dog Populations: A Review Of Products And Criteria For Field Applications, Giovanna Massei, Lowell A. Miller Jan 2013

Nonsurgical Fertility Control For Managing Free-Roaming Dog Populations: A Review Of Products And Criteria For Field Applications, Giovanna Massei, Lowell A. Miller

United States Department of Agriculture Wildlife Services: Staff Publications

About 75% of dogs worldwide are free to roam and reproduce, thus creating locally overabundant populations. Problems caused by roaming dogs include diseases transmitted to livestock and humans, predation on livestock, attacks on humans, road traffic accidents, and nuisance behavior. Nonsurgical fertility control is increasingly advocated as more cost-effective than surgical sterilization to manage dog populations and their impact. The aims of this review were to 1) analyze trends in numbers of scientific publications on nonsurgical fertility control for dogs; 2) illustrate the spectrum of fertility inhibitors available for dogs; 3) examine how differences between confined and free-roaming dogs might …


Changes In Grouping Patterns Of Saiga Antelope In Relation To Intrinsic And Environmental Factors In Mongolia, B. B. Buuveibaatar, T. K. Fuller, A. E. Fine, B. Chimeddorj, J. K. Young, J. Berger Jan 2013

Changes In Grouping Patterns Of Saiga Antelope In Relation To Intrinsic And Environmental Factors In Mongolia, B. B. Buuveibaatar, T. K. Fuller, A. E. Fine, B. Chimeddorj, J. K. Young, J. Berger

United States Department of Agriculture Wildlife Services: Staff Publications

Factors that affect group sizes in large ungulates are generally poorly understood for species from remote regions. Understanding grouping patterns is important for effective species management, but is lacking for the endangered Mongolian saiga (Saiga tatarica mongolica).We studied seasonal changes in the group size and social structure of saigas in relation to environmental and anthropogenic factors in western Mongolia during 2009–2012. To identify group size and composition, we observed saigas monthly while conducting monitoring surveys, and weekly while tracking radio-collared animals. We observed 9268 individuals; median group size was 6.5 (SE = 1.7; range = 1–121), and groups …


The Effect Of Social Hierarchy On Captive Coyote (Canis Latrans) Foraging Behavior, Lynne B. Gilbert-Norton, Ryan R. Wilson, John A. Shivik Jan 2013

The Effect Of Social Hierarchy On Captive Coyote (Canis Latrans) Foraging Behavior, Lynne B. Gilbert-Norton, Ryan R. Wilson, John A. Shivik

United States Department of Agriculture Wildlife Services: Staff Publications

Foraging efficiency of individuals in pack forming species may be influenced by social dynamics within a pack. The effects of social hierarchy in particular may influence individual foraging behavior in canids, such as coyotes (Canis latrans). To examine the impact of social hierarchy on foraging behavior, we tested 16 captive coyotes in eight naturally established dominant–subordinate pairs, using the guesser–knower paradigm. We measured the efficiency of subordinate coyotes to relocate a food resource when alone and then allowed pairs to forage together, such that subordinates had prior knowledge of food location but dominants did not. To determine whether …


Functional Significance Of Ultraviolet Feeding Cues In Wild Turkeys, Scott J. Werner, Richard Buchholz, Shelagh K. Tupper, Susan E. T. Pettit, Jeremy W. Ellis Jan 2013

Functional Significance Of Ultraviolet Feeding Cues In Wild Turkeys, Scott J. Werner, Richard Buchholz, Shelagh K. Tupper, Susan E. T. Pettit, Jeremy W. Ellis

United States Department of Agriculture Wildlife Services: Staff Publications

Most birds are able to sense ultraviolet (UV) visual signals. Ultraviolet wavelengths are used for plumage signaling and sexual selection among birds. The aim of our study was to determine if UV cues are also used for the process of food selection in wild turkeys (Meleagris gallopavo). We used avoidance conditioning to test the hypothesis that UV feeding cues can be used functionally for foraging behavior in wild turkeys. Female turkeys exhibited no avoidance of untreated food and 75–98% avoidance of food treated with an UV-absorbent, postingestive repellent (0.5–4% anthraquinone; wt./wt.) during repellent exposure. Male turkeys exhibited 78–99% …


Benzo[A]Pyrene Effects On Fundulus Heteroclitus Reproductive Endpoints, Frank Booc Jan 2013

Benzo[A]Pyrene Effects On Fundulus Heteroclitus Reproductive Endpoints, Frank Booc

Electronic Theses and Dissertations

Benzo[a]pyrene (BaP) is a polycyclic aromatic hydrocarbon (PAH) that modulates aromatase enzyme function and, thus, potentially interrupts normal reproductive function. The aim of this study was to use a fish model, Fundulus heteroclitus, to assess whether BaP exposure adversely impacted reproduction. Adult fish were exposed to waterborne BaP concentrations of (0, 1 or 10 μg/L) for 28 days. Males and females were combined for the second half of the exposure (days 14-28) in order to quantitate egg production and fertilization success. Egg fertilization and subsequent hatching success of F1 embryos was significantly decreased by 10 μg/L BaP. In males, both …


Snp-Snp Interaction Network In Angiogenesis Genes Associated With Prostate Cancer Aggressiveness, Hui-Yi Lin, Ernest K. Amankwah, Tung-Sung Tseng, Xiaotao Qu, Dung-Tsa Chen, Jong Y. Park Jan 2013

Snp-Snp Interaction Network In Angiogenesis Genes Associated With Prostate Cancer Aggressiveness, Hui-Yi Lin, Ernest K. Amankwah, Tung-Sung Tseng, Xiaotao Qu, Dung-Tsa Chen, Jong Y. Park

School of Public Health Faculty Publications

Angiogenesis has been shown to be associated with prostate cancer development. The majority of prostate cancer studies focused on individual single nucleotide polymorphisms (SNPs) while SNP-SNP interactions are suggested having a great impact on unveiling the underlying mechanism of complex disease. Using 1,151 prostate cancer patients in the Cancer Genetic Markers of Susceptibility (CGEMS) dataset, 2,651 SNPs in the angiogenesis genes associated with prostate cancer aggressiveness were evaluated. SNP-SNP interactions were primarily assessed using the two-stage Random Forests plus Multivariate Adaptive Regression Splines (TRM) approach in the CGEMS group, and were then re-evaluated in the Moffitt group with 1,040 patients. …


Differential Bait Preference And Rate Of Attraction By Argentine Ants (Linepithema Humile Mayr) At Freshwater And Saltwater Marsh Sites In Southern California, Victor D. Carmona-Galindo, Meghan Moore, Melanie Tansuwan Jan 2013

Differential Bait Preference And Rate Of Attraction By Argentine Ants (Linepithema Humile Mayr) At Freshwater And Saltwater Marsh Sites In Southern California, Victor D. Carmona-Galindo, Meghan Moore, Melanie Tansuwan

Biology Faculty Works

Ants are a type of foraging insect species which harvests food resources based on availability. When ants locate food resources that are scarce within their habitat, they tend to be more strongly attracted to that food resource. This study used protein, carbohydrate and control based baits to examine if there was a deficiency in resources demonstrated by the ants at two different wetland habitats. We sampled Argentine ants (Linepithema humileMayr) within the saltwater and freshwater marshes of Ballona Wetlands in Los Angeles, CA. We found significant differences in the rapid deployment of Argentine ants towards protein baits over …


Chemistry And Microbial Functional Diversity Differences In Biofuel Crop And Grassland Soils In Multiple Geographies, Lidia S. Watrud, Jay R. Reichman, Michael A. Bollman, Bonnie M. Smith, E. Henry Lee, Julie D. Jastrow, Michael D. Casler, Harold P. Collins, Steven Fransen, Robert B. Mitchell, Vance N. Owens, Brent Bean, William L. Rooney, Donald D. Tyler, George A. King Jan 2013

Chemistry And Microbial Functional Diversity Differences In Biofuel Crop And Grassland Soils In Multiple Geographies, Lidia S. Watrud, Jay R. Reichman, Michael A. Bollman, Bonnie M. Smith, E. Henry Lee, Julie D. Jastrow, Michael D. Casler, Harold P. Collins, Steven Fransen, Robert B. Mitchell, Vance N. Owens, Brent Bean, William L. Rooney, Donald D. Tyler, George A. King

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

We obtained soil samples from geographically diverse switchgrass (Panicum virgatum L.) and sorghum (Sorghum bicolor L.) crop sites and from nearby reference grasslands and compared their edaphic properties, microbial gene diversity and abundance, and active microbial biomass content. We hypothesized that soils under switchgrass, a perennial, would be more similar to reference grassland soils than sorghum, an annual crop. Sorghum crop soils had significantly higher NO3 −-N, NH4 +-N, SO4 2−-S, and Cu levels than grassland soils. In contrast, few significant differences in soil chemistry were observed between switchgrass crop and grassland …


Impacts Of Elevated Co2 Concentration On The Productivity And Surface Energy Budget Of The Soybean And Maize Agroecosystem In The Midwest Usa, Tracy E. Twine, Jarod J. Bryant, Katherine T. Richter, Carl J. Bernacchi, Kelly D. Mcconnaughay, Sherri J. Morris, Andrew D. B. Leakey Jan 2013

Impacts Of Elevated Co2 Concentration On The Productivity And Surface Energy Budget Of The Soybean And Maize Agroecosystem In The Midwest Usa, Tracy E. Twine, Jarod J. Bryant, Katherine T. Richter, Carl J. Bernacchi, Kelly D. Mcconnaughay, Sherri J. Morris, Andrew D. B. Leakey

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The physiological response of vegetation to increasing atmospheric carbon dioxide concentration ([CO2]) modifies productivity and surface energy and water fluxes. Quantifying this response is required for assessments of future climate change. Many global climate models account for this response; however, significant uncertainty remains in model simulations of this vegetation response and its impacts. Data from in situ field experiments provide evidence that previous modeling studies may have overestimated the increase in productivity at elevated [CO2], and the impact on large-scale water cycling is largely unknown. We parameterized the Agro-IBIS dynamic global vegetation model with observations from …


Comparative Analysis Of Edwardsiella Isolates From Fish In The Eastern United States Identifies Two Distinct Genetic Taxa Amongst Organisms Phenotypically Classified As E. Tarda, Matt J. Griffin, Sylvie M. Quiniou, Theresa Cody, Maki Tabuchi, Cynthia Ware, Rocco C. Cipriano, Michael J. Mauel, Esteban Soto Jan 2013

Comparative Analysis Of Edwardsiella Isolates From Fish In The Eastern United States Identifies Two Distinct Genetic Taxa Amongst Organisms Phenotypically Classified As E. Tarda, Matt J. Griffin, Sylvie M. Quiniou, Theresa Cody, Maki Tabuchi, Cynthia Ware, Rocco C. Cipriano, Michael J. Mauel, Esteban Soto

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Edwardsiella tarda, a Gram-negative member of the family Enterobacteriaceae, has been implicated in significant losses in aquaculture facilities worldwide. Here, we assessed the intra-specific variability of E. tarda isolates from 4 different fish species in the eastern United States. Repetitive sequence mediated PCR (rep-PCR) using 4 different primer sets (ERIC I & II, ERIC II, BOX, and GTG5) and multi-locus sequence analysis of 16S SSU rDNA, groEl, gyrA, gyrB, pho, pgi, pgm, and rpoA gene fragments identified two distinct genotypes of E. tarda (DNA group I; DNA group II). Isolates that fell into DNA group II demonstrated more similarity …


Dynamics Of Plant Root Growth Under Increased Atmospheric Carbon Dioxide, M. Madhu, J. L. Hatfield Jan 2013

Dynamics Of Plant Root Growth Under Increased Atmospheric Carbon Dioxide, M. Madhu, J. L. Hatfield

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Plant growth is influenced by above- and belowground environmental conditions and increasing atmospheric carbon dioxide (CO2) concentrations enhances growth and yield of most agricultural crops. This review covers current knowledge on the impact of increasing CO2 concentration on root dynamics of plants in terms of growth, root/shoot (R/S) ratio, root biomass, root length, root longevity, root mortality, root distribution, root branching, root quality, and the response of these root parameters to management practices including soil water and nutrients. The effects of CO2 concentration on R/S ratio are contradictory due to complexity in accurate underground biomass estimation …


Lysine Optimization Of A Commercial Fishmeal-Free Diet For Hybrid Striped Bass (Morone Chrysops × M. Saxatilis), Steven D. Rawles, S. Adam Fuller, Benjamin H. Beck, T. Gibson Gaylord, Frederic T. Barrows, Matthew E. Mcentire Jan 2013

Lysine Optimization Of A Commercial Fishmeal-Free Diet For Hybrid Striped Bass (Morone Chrysops × M. Saxatilis), Steven D. Rawles, S. Adam Fuller, Benjamin H. Beck, T. Gibson Gaylord, Frederic T. Barrows, Matthew E. Mcentire

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Substitution of fishmeal with alternate proteins in aquafeeds often results in dietary imbalances of first-limiting essential amino acids (EAA) and poorer fish performance. This growth trial was undertaken to test the hypothesis that ideal protein theory accurately predicts first-limiting amino acids and optimumlysine level for a fishmeal-free, commercial-grade diet formulated for hybrid striped bass (HSB). The ideal model for formulation was the amino acid pattern of hybrid striped bass muscle. A negative control diet based on soybean (45%), corn gluten (10%), and poultry by-product (13%) meals was formulated on an ideal basis to contain 18% lipid, 40% crude protein (CP), …


Functional Characterization Of Cinnamyl Alcohol Dehydrogenase And Caffeic Acid O-Methyltransferase In Brachypodium Distachyon, Gina M. Trabucco, Dominick A. Matos, Scott J. Lee, Aaron J. Saathoff, Henry D. Priest, Todd C. Mockler, Gautam Sarath, Samuel P. Hazen Jan 2013

Functional Characterization Of Cinnamyl Alcohol Dehydrogenase And Caffeic Acid O-Methyltransferase In Brachypodium Distachyon, Gina M. Trabucco, Dominick A. Matos, Scott J. Lee, Aaron J. Saathoff, Henry D. Priest, Todd C. Mockler, Gautam Sarath, Samuel P. Hazen

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Background: Lignin is a significant barrier in the conversion of plant biomass to bioethanol. Cinnamyl alcohol dehydrogenase (CAD) and caffeic acid O-methyltransferase (COMT) catalyze key steps in the pathway of lignin monomer biosynthesis. Brown midrib mutants in Zea mays and Sorghum bicolor with impaired CAD or COMT activity have attracted considerable agronomic interest for their altered lignin composition and improved digestibility. Here, we identified and functionally characterized candidate genes encoding CAD and COMT enzymes in the grass model species Brachypodium distachyon with the aim of improving crops for efficient biofuel production.

Results: We developed transgenic plants overexpressing artificial microRNA designed …


Evaluation Of Response To Bovine Viral Diarrhea Virus Type 2 Vaccination And Timing Of Weaning On Yearling Ultrasound Body Composition, Performance, And Carcass Quality Traits In Angus Calves, R. G. Tait Jr, E. D. Downey, M. S. Mayes, C. A. Park, J. F. Ridpath, D. J. Garrick, J. M. Reecy Jan 2013

Evaluation Of Response To Bovine Viral Diarrhea Virus Type 2 Vaccination And Timing Of Weaning On Yearling Ultrasound Body Composition, Performance, And Carcass Quality Traits In Angus Calves, R. G. Tait Jr, E. D. Downey, M. S. Mayes, C. A. Park, J. F. Ridpath, D. J. Garrick, J. M. Reecy

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

There are concerns about antagonisms between immunity and animal productivity in livestock production. The objective of this study was to evaluate the effect of antibody levels through a response to vaccination protocol, weaning timing, and their interaction on performance and carcass quality traits in Angus beef cattle. Final antibody level and response to vaccination were based on neutralizing serum antibodies against bovine viral diarrhea virus type 2 (BVDV2). Calves were followed through development and the feedlot phase, with collection of yearling ultrasound (n = 957), preharvest (n = 762), and carcass (n = 673) data. In this …


An Abundant Biological Control Agent Does Not Provide A Significant Predator Subsidy, Philip Tipping, Melissa Martin, Paul Pratt, Min Rayamajhi, Ted Center Jan 2013

An Abundant Biological Control Agent Does Not Provide A Significant Predator Subsidy, Philip Tipping, Melissa Martin, Paul Pratt, Min Rayamajhi, Ted Center

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Classical weed biological control agents, regardless of their effectiveness, may provide subsidies to predators and parasites. The chemically defended weevil Oxyops vitiosa Pascoe is a successful agent that was introduced to control the invasive tree Melaleuca quinquenervia. Two consecutive small plot experiments that lasted two and three years, respectively, examined the population dynamics of O. vitiosa while subjected to predation by Podisus mucronatus, a native generalist predator. During this time the estimated mean (±SE) percent predation of O. vitiosa larvae was 7.2 ± 1.7% per sample date in the two year study and 8.4 ± 0.8% in the three year …


Editorial: Introduction To Themed Section— Supporting Ecosystem Services With Conservation Agricultural Approaches, Alan J. Franzluebbers Jan 2013

Editorial: Introduction To Themed Section— Supporting Ecosystem Services With Conservation Agricultural Approaches, Alan J. Franzluebbers

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Ecosystem services are the properties and processes of the natural world that contribute to the well-being of plants, animals and humans in a holistic and global context. For too long, members of the agricultural community have been solely focused on the provision of food, feed and fiber. Of course, this essential human innovation has provided hugely important products engineered for mass production that serves all of human society. However, agriculture and the ecosystems in which it is practiced provide numerous other services that are becoming increasingly relevant to its very survival and the survival of humans that it supports. Several …


Soil Quality Response To Long-Term Tillage And Crop Rotation Practices, Douglas Karlen, Cynthia A. Cambardella, John L. Kovar, Thomas S. Colvin Jan 2013

Soil Quality Response To Long-Term Tillage And Crop Rotation Practices, Douglas Karlen, Cynthia A. Cambardella, John L. Kovar, Thomas S. Colvin

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Soil quality is influenced by inherent and anthropogenic factors. This study was conducted to provide multiple groups guidance on how to achieve and maintain improved soil quality/health. Our hypothesis was that tillage intensity was the primary anthropogenic factor degrading soil quality, and our objective was to prove that hypothesis through an intensive 2005 sampling of a central Iowa, USA field study. Chisel plow, disk tillage, moldboard plow, ridge-till and no-till treatments, used for 31 years in a twoyear, corn (Zea mays L.)/soybean [Glycine max (L.) Merr.] (C/S) rotation or for 26 years of continuous corn (CC) production, were …


Elucidation Of The Structure And Reaction Mechanism Of Sorghum Hydroxycinnamoyltransferase And Its Structural Relationship To Other Coenzyme A-Dependent Transferases And Synthases, Alexander M. Walker, Robert P. Hayes, Buhyun Youn, Wilfred Vermerris, Scott E. Sattler, Chulhee Kang Jan 2013

Elucidation Of The Structure And Reaction Mechanism Of Sorghum Hydroxycinnamoyltransferase And Its Structural Relationship To Other Coenzyme A-Dependent Transferases And Synthases, Alexander M. Walker, Robert P. Hayes, Buhyun Youn, Wilfred Vermerris, Scott E. Sattler, Chulhee Kang

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Hydroxycinnamoyltransferase (HCT) from sorghum (Sorghum bicolor) participates in an early step of the phenylpropanoid pathway, exchanging coenzyme A (CoA) esterified to p-coumaric acid with shikimic or quinic acid as intermediates in the biosynthesis of the monolignols coniferyl alcohol and sinapyl alcohol. In order to elucidate the mode of action of this enzyme, we have determined the crystal structures of SbHCT in its apo-form and ternary complex with shikimate and p-coumaroyl-CoA, which was converted to its product during crystal soaking. The structure revealed the roles of threonine-36, serine-38, tyrosine- 40, histidine-162, arginine-371, and threonine-384 in catalysis and specificity. Based …


Using A Community-Based Participatory Research Approach To Address Individual And Community Health In Charleston, Ms. The Initial Step: A Formative Needs Assessment, Catherine Dane Woodyard Jan 2013

Using A Community-Based Participatory Research Approach To Address Individual And Community Health In Charleston, Ms. The Initial Step: A Formative Needs Assessment, Catherine Dane Woodyard

Electronic Theses and Dissertations

The purpose of this formative study was to use a Community-Based Participatory Research (CBPR) approach to conduct a comprehensive health needs assessment (CHNA) in Charleston, Mississippi (MS) to determine perceived needs of the community and identify priority health issues. A second purpose was to provide methodological and scholarly contributions to the existing literature regarding CBPR and CHNAs. The target population was residents living in Charleston, MS (N=2,193). Eighty-five residents participated in the study. This was a five-part study including: (1) Key informant interviews (n=11), (2) Focus groups (n=8), (3) Assessment of the built environment, (4) Assessment of the nutrition environment, …


Impact Of Temperature And Relative Humidity On Life History Parameters Of Adult Sitotroga Cerealella (Lepidoptera: Gelechiidae), James E. Throne, David K. Weaver Jan 2013

Impact Of Temperature And Relative Humidity On Life History Parameters Of Adult Sitotroga Cerealella (Lepidoptera: Gelechiidae), James E. Throne, David K. Weaver

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The Angoumois grain moth, Sitotroga cerealella (Olivier) (Lepidoptera: Gelechiidae), is a pest of stored corn, Zea mays L., and other grains throughout the world. Sitotroga cerealella are routinely exposed to temperatures below 20°C in regions of the U.S. where corn is grown, yet there are no data describing adult life history parameters below 20°C. We determined longevity, fecundity, and survivorship of eggs at a range of temperatures that represent environmental conditions to which S. cerealella are exposed in corn stored in the U.S. Longest male longevity was 31 d at 10°C, and shortest male longevity was 4 d at 35 …


Development And Prospect Of Unmanned Aerial Vehicle Technologies For Agricultural Production Management, Yanbo Huang, Steven J. Thomson, W. Clint Hoffmann, Yubin Lan, Bradley K. Fritz Jan 2013

Development And Prospect Of Unmanned Aerial Vehicle Technologies For Agricultural Production Management, Yanbo Huang, Steven J. Thomson, W. Clint Hoffmann, Yubin Lan, Bradley K. Fritz

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Unmanned aerial vehicles have been developed and applied to support agricultural production management. Compared with piloted aircraft, an Unmanned Aerial Vehicle (UAV) can focus on small crop fields at lower flight altitudes than regular aircraft to perform site-specific farm management with higher precision. They can also “fill in the gap” in locations where fixed winged or rotary winged aircraft are not readily available. In agriculture, UAVs have primarily been developed and used for remote sensing and application of crop production and protection materials. Application of fertilizers and chemicals is frequently needed at specific times and locations for site-specific management. Routine …