Digital Commons Network^™

Impact Of Temperature And Relative Humidity On Life History Parameters Of Adult Sitotroga Cerealella (Lepidoptera: Gelechiidae), James E. Throne, David K. Weaver

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The Angoumois grain moth, Sitotroga cerealella (Olivier) (Lepidoptera: Gelechiidae), is a pest of stored corn, Zea mays L., and other grains throughout the world. Sitotroga cerealella are routinely exposed to temperatures below 20°C in regions of the U.S. where corn is grown, yet there are no data describing adult life history parameters below 20°C. We determined longevity, fecundity, and survivorship of eggs at a range of temperatures that represent environmental conditions to which S. cerealella are exposed in corn stored in the U.S. Longest male longevity was 31 d at 10°C, and shortest male longevity was 4 d at 35 …

Development And Prospect Of Unmanned Aerial Vehicle Technologies For Agricultural Production Management, Yanbo Huang, Steven J. Thomson, W. Clint Hoffmann, Yubin Lan, Bradley K. Fritz

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Unmanned aerial vehicles have been developed and applied to support agricultural production management. Compared with piloted aircraft, an Unmanned Aerial Vehicle (UAV) can focus on small crop fields at lower flight altitudes than regular aircraft to perform site-specific farm management with higher precision. They can also “fill in the gap” in locations where fixed winged or rotary winged aircraft are not readily available. In agriculture, UAVs have primarily been developed and used for remote sensing and application of crop production and protection materials. Application of fertilizers and chemicals is frequently needed at specific times and locations for site-specific management. Routine …

Disparate Host Immunity To Mycobacterium Avium Subsp. Paratuberculosis Antigens In Calves Inoculated With M. Avium Subsp. Paratuberculosis, M. Avium Subsp. Avium, M. Kansasii, And M. Bovis, Judith R. Stabel, W.R. Waters, John Bannantine, M.V. Palmer

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The cross-reactivity of mycobacterial antigens in immune-based diagnostic assays has been a major concern and a criticism of the current tests that are used for the detection of paratuberculosis. In the present study, Mycobacterium avium subsp. paratu- berculosis recombinant proteins were evaluated for antigenic specificity compared to a whole-cell sonicate preparation (MPS). Measures of cell-mediated immunity to M. avium subsp. paratuberculosis antigens were compared in calves inoculated with live M. avium subsp. paratuberculosis, M. avium subsp. avium (M. avium), Mycobacterium kansasii, or Mycobacterium bovis. Gamma interferon (IFN-􏰋) responses to MPS were observed in all calves that were exposed to mycobacteria …

No Holes Barred: Invasion Of The Intestinal Mucosa By Mycobacterium Avium Subsp. Paratuberculosis, John Bannantine, Luiz E. Bermudez

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The infection biology of Mycobacterium avium subsp. paratuberculosis has recently crystallized, with added details surrounding intestinal invasion. The involvement of pathogen-derived effector proteins such as the major membrane protein, oxidoreductase, and fibronectin attachment proteins have been uncovered. Mutations constructed in this pathogen have also shed light on genes needed for invasion. The host cell types that are susceptible to invasion have been defined, along with their transcriptional response. Recent details have given a new appreciation for the dynamic interplay between the host and bacterium that occurs at the outset of infection. An initial look at the global expression pathways of …

Microbial Community Analysis Of Swine Wastewater Anaerobic Lagoons By Next-Generation Dna Sequencing, Thomas F. Ducey, Patrick G. Hunt

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Anaerobic lagoons are a standard practice for the treatment of swine wastewater. This practice relies heavily on microbiological processes to reduce concentrated organic material and nutrients. Despite this reliance on microbiological processes, research has only recently begun to identify and enumerate the myriad and complex interactions that occur in this microbial ecosystem. To further this line of study, we utilized a next-generation sequencing (NGS) technology to gain a deeper insight into the microbial communities along the water column of four anaerobic swine wastewater lagoons. Analysis of roughly one million 16S rDNA sequences revealed a predominance of operational taxonomic units (OTUs) …

Creation Of A High-Amylose Durum Wheat Through Mutagenesis Of Starch Synthase Ii (Ssiia), A.C. Hogg, K. Gause, P. Hofer, J.M. Martin, Robert A. Graybosch, L.E. Hansen, M.J. Giroux

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

In cereal seeds, mutations in one or more starch synthases lead to decreased amylopectin and increased amylose content. Here, the impact of starch synthase IIa (SSIIa or SGP-1) mutations upon durum starch was investigated. A screen of durum accessions identified two lines lacking SGP-A1, the A genome copy of SGP-1. The two lines were determined to carry a 29 bp deletion in the first exon of SSIIa. The SGP-A1 nulls were crossed with the durum variety ‘Mountrail’ and F₅ derived SGP-A1 null progeny lines were treated with EMS. From each EMS population, one SGP-B1 null mutation …

Use Of Dairies By Postreproductive Flocks Of European Starlings, H. J. Homan, Jeffrey T. Lejeune, David L. Pearl, T. W, Seamans, A. A. Slowik, Mark R. Morasch, G. M. Linz

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Knowledge of the behavior and movement patterns of European starlings (Sturnus vulgaris L.) is important to wildlife managers that seek to resolve conflicts at livestock facilities. We captured and radio tagged 10 starlings at each of 5 dairies in northeastern Ohio. From September 19 to October 31, 2007, we obtained sufficient data from 40 birds to study their behavior and movements. The birds visited the dairies where they were initially captured (home sites) on 85% of the days, spending 58% of each day at the dairies. Onsite arrival and departure times were 2.5 h after sunrise and 3.1 h …

Host Location By Spalangia Cameroni (Hymenoptera: Pteromalidae) In Equine Associated Substrates, Erika T. Machtinger, Christopher J. Geden

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

House flies, (Musca domestica L.) and stable flies (Stomoxys calcitrans L.) are common pests on equine facilities. Biological control of these flies with pupal parasitoids has become increasingly popular with horse owners but has not been evaluated on equine facilities. Little is known of the substrate preferences of filth fly parasitoids on equine facilities, but the success of release programs may be affected by microhabitat preferences. Spalangia cameroni Perkins was evaluated for location preferences for parasitization of house fly and stable fly puparia in six substrates commonly found on equine farms in Florida. Substrates were evaluated at 20:1 …

Geomorphic-Vegetation Relationships Using A Geopedological Classification System, Northern Chihuahuan Desert, Usa, G.A. Michaud, H.C. Monger, D.L. Anderson

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Drylands typically have distinctive vegetative patterns that reflect the underlying physical landscape. We use a geopedological classification to organize the landscape into five categories from broad scale to fine scale: (1) physiographic divisions which describe regional topography, such as mountains and basin floors; (2) regolith type which identifies residuum versus transported sediments; (3) parent materials which describe mineralogy; (4) landforms which provide meso-scale descriptions of topography; and (5) soil texture, a fine-scale variable important for infiltration, erodibility, and available water holding capacity. The study was conducted in a 1753 km² area of the White Sands Missile Range and …

Qualitative And Quantitative Analysis Of Lignocellulosic Biomass Using Infrared Techniques: A Mini-Review, Feng Xu, Jianming Yu, Tesfaye Tesso, Floyd Dowell, Donghai Wang

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Current wet chemical methods for biomass composition analysis using two-step sulfuric acid hydrolysis are time-consuming, labor-intensive, and unable to provide structural information about biomass. Infrared techniques provide fast, low-cost analysis, are non-destructive, and have shown promising results. Chemometric analysis has allowed researchers to perform qualitative and quantitative study of biomass with both near-infrared and mid-infrared spectroscopy. This review summarizes the progress and applications of infrared techniques in biomass study, and compares the infrared and the wet chemical methods for composition analysis. In addition to reviewing recent studies of biomass structure and composition, we also discuss the progress and prospects for …

Altered Physiology, Cell Structure, And Gene Expression Of Theobroma Cacao Seedlings Subjected To Cu Toxicity, Vânia L. Souza, Alex-Alan F. De Almeida, Jadiel De S. Souza, Pedro A. O. Mangabeira, Raildo M. De Jesus, Carlos P. Pirovani, Dário Ahnert, Virupax C. Baligar, Leandro L. Loguercio

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Seedlings of Theobroma cacao CCN 51 genotype were grown under greenhouse conditions and exposed to increasing concentrations of Cu (0.005, 1, 2, 4, 8, 16, and 32 mg Cu L⁻¹) in nutrient solution. When doses were equal or higher than 8mg Cu L⁻¹, after 24 h of treatment application, leaf gas exchange was highly affected and changes in chloroplasts thylakoids of leaf mesophyll cells and plasmolysis of cells from the root cortical region were observed. In addition, cell membranes of roots and leaves were damaged. In leaves, 96 h after treatments started, increases in the percentage …

Discovery Of Single Nucleotide Polymorphisms In Candidate Genes Associated With Fertility And Production Traits In Holstein Cattle, Sarah D. Cochran, John B. Cole, Daniel J. Null, Peter J. Hansen

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Background: Identification of single nucleotide polymorphisms (SNPs) for specific genes involved in reproduction might improve reliability of genomic estimates for these low-heritability traits. Semen from 550 Holstein bulls of high (≥ 1.7; n = 288) or low (≤ −2; n = 262) daughter pregnancy rate (DPR) was genotyped for 434 candidate SNPs using the Sequenom MassARRAY^® system. Three types of SNPs were evaluated: SNPs previously reported to be associated with reproductive traits or physically close to genetic markers for reproduction, SNPs in genes that are well known to be involved in reproductive processes, and SNPs in genes that …

Identifying Inter- And Intra-Guild Feeding Activity Of An Arthropod Predator Assemblage, James R. Hagler, Felisa Blackmer

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

1. Understanding predator–prey interactions of the arthropod community in any given ecosystem is essential in pinpointing the biological control services provided by natural enemies.

2. Hence, four prey-specific polymerase chain reaction (PCR) assays were developed to analyse the gut contents of the cotton predator community. The four targeted prey included a herbivore/pest, omnivore/pest, omnivore/beneficial, and carnivore/beneficial.

3. First, prey retention tests were conducted to determine how long a prey item of each target species could be detected in a predator after ingestion. The assays yielded highly variable inter-assay and intra-assay prey detection efficiencies.

4. Then, a multifaceted field study was …

Wool Carder Bees Of The Genus Anthidium In The Western Hemisphere (Hymenoptera: Megachilidae): Diversity, Host Plant Associations, Phylogeny, And Biogeography, Victor Gonzalez, Terry Griswold

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Bees are among the most important pollinators of flowering plants in most ecosystems. Recent concerns about population decline worldwide have highlighted the urgent societal need for species-level systematic work that facilitates assessments of the status of pollinators and pollination services. This is a comprehensive, broadly comparative study on the diversity, biology, biogeography, and evolution of Anthidium Fabricius, 1804, one of the most diverse megachilid genera, containing more than 160 species worldwide. Herein, the Western Hemisphere species are revised. All 92 recognized species, including the two adventive species Anthidium oblongatum (Illiger, 1806) and Anthidium manicatum (Linnaeus, 1758), are described and …

Regional Scale Patterns Of Fine Root Lifespan And Turnover Under Current And Future Climate, M. Luke Mccormack, David M. Eissenstat, Anantha M. Prasad, Erica A. H. Smithwick

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Fine root dynamics control a dominant flux of carbon from plants and into soils and mediate potential uptake and cycling of nutrients and water in terrestrial ecosystems. Understanding of these patterns is needed to accurately describe critical processes like productivity and carbon storage from ecosystem to global scales. However, limited observations of root dynamics make it difficult to define and predict patterns of root dynamics across broad spatial scales. Here, we combine species-specific estimates of fine root dynamics with a model that predicts current distribution and future suitable habitat of temperate tree species across the eastern United States (US). Estimates …

Volatile Induction Of Infected And Neighbouring Uninfected Plants Potentially Influence Attraction/Repellence Of A Cereal Herbivore, D. Piesik, D. Pańka, M. Jeske, A. Wenda-Piesik, K. J. Delaney, David K. Weaver

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Pathogen infection can induce plant volatile organic compounds (VOCs). We infected ‘McNeal’ wheat and ‘Harrington’ barley with a Fusarium spp. blend (F. graminearum, F. avenaceum and F. culmorum). Both cereals had the greatest VOC induction 14 days after pathogen innoculation, only slightly lower induction occurred at 7 days, but displayed no induction at 1 days. The induced VOC bouquet for both cereals included six green leaf volatiles (GLVs; e.g. (Z)-3-hexenol and (Z)-3-hexenyl acetate), four terpenes (linalool, linalool oxide, (Z)-β-ocimene and (E)-β-caryophyllene) and benzyl acetate. Neighboring, uninfected individuals …

Processing Of Affective Faces Varying In Valence And Intensity In Shy Adults: An Event-Related Fmri Study, Erica Tatham, Louis A. Schmidt, Elliott A. Beaton, Jay Schulkin, Geoffrey B. Hall

Psychology Faculty Publications

Recent behavioral and electrocortical studies have found that shy and socially anxious adults are hypersensitive to the processing of negative and ambiguous facial emotions. We attempted to extend these findings by examining the neural correlates of affective face processing in shy adults using an event-related fMRI design. We presented pairs of faces that varied in affective valence and intensity. The faces were morphed to alter the degree of intensity of the emotional expressive faces. Twenty-four (12 shy and 12 non-shy) young adult participants then made same/different judgments to these faces while in an MR scanner. We found that shy adults …

Development Of Thulium Fiber Lasers For High Average Power And High Peak Power Operation, Robert Sims

Electronic Theses and Dissertations

High power thulium fiber lasers are useful for a number of applications in both continuous-wave and pulsed operating regimes. The use of thulium as a dopant has recently gained interest due to its large bandwidth, possibility of high efficiency, possibility of high power and long wavelength ~1.8 – 2.1 μm. The longer emission wavelength of Tm-doped fiber lasers compared to Yb- and/or Er-doped fiber lasers creates the possibility for higher peak power operation due to the larger nonlinear thresholds and reduced nonlinear phase accumulation. One primary interest in Tm-doped fiber lasers has been to scale to high average powers; however, …

Human Cytomegalovirus Us17 Locus Fine-Tunes Innate And Intrinsic Immune Responses, Stephen James Gurczynski

Wayne State University Dissertations

HCMV employs numerous strategies to combat, subvert, or co-opt host immunity. One evolutionary strategy for this involves "capture" of a host gene and then its successive duplication and divergence, forming a gene family, many of which have immunomodulatory activities. The HCMV US12 family consists of ten tandemly arranged sequence-related genes in the unique short region of the HCMV genome (US12-US21). Each gene encodes a protein possessing seven predicted transmembrane domains, and patches of sequence similarity with cellular GPCRs and the bax inhibitor-1 family of anti-apoptotic proteins. We show that one member, US17, plays an important role during virion maturation. Microarray …

Platyrrhine Phylogenetics With A Focus On Callitrichine Life History Adaptations, Natalie Mae Jameson

Wayne State University Dissertations

The life history of a species is highly impacted by their reproductive strategy. In my dissertation I address the changing reproductive strategies in callitrichine New World monkeys and their genetic underpinnings using a phylogenetic approach. The necessity for a resolved phylogeny is universal to any comparative genomic study. Here we have constructed a reliable phylogenetic framework from which reproductive strategy could be studied in callitrichines. First, to determine the most recent common ancestor of Anthropoid primates we took a phylogenomic approach, using the publicly available whole genome sequences of 17 mammal species. With high confidence, we determined here that Tarsier …

An Exploration Of The Perceived Academic Self-Efficacy And Locus Of Control Of Urban Students With Learning Disabilities, Olukayode Abimbola Nuga

Wayne State University Dissertations

The search for an answer to the question of the achievement gap between Black and other minority students and their White counterparts has been pervasive. This study, however, reframes the question not by using the White student as a frame of reference against which to make the comparison but by using the Black student himself. Therefore, relying on some scholarly work that found that studies of achievement within ethnic groups yielded far more significant results than between groups, the study focused on a comparison of the academic achievement between urban Black students with learning disabilities and their typical peers. The …

Analysis Of The Structure And Function Of A Timp-1/Cd63 Complex And Its Relationship To An Mt1-Mmp/Cd63 Complex, Richard B. Warner

Wayne State University Dissertations

The relationship of the extra-cellular matrix (ECM) and secreted proteins to intra-cellular functions has an important impact on disease progression. The tissue inhibitors of metalloproteinases (TIMPs), of which there are four, are known for their broad metalloprotease inhibitory abilities. TIMP-1 has been widely studied as an inhibitor of matrix metalloproteinases (MMPs) and therefore as a molecule that prevents ECM degradation and proteolytic cleavage of extra-cellular molecules, processes thought to be critical for tumor invasion and metastasis. However, TIMP-1 has shown in the clinic to have increased expression in cases of disease progression; especially, it is associated with poor prognosis in …

An Investigation Into The Role Of Cfia 3' End Processing Complex In The Termination And Initiation/Reinitiation Of Transcription, Nadra Al-Husini

Wayne State University Dissertations

In budding yeast, as in higher eukaryotes, transcription of protein coding genes is executed by a highly specialized, conserved polymerase called RNA polymerase II (RNAPII). The transcription cycle of RNAPII has four major steps: initiation, elongation, termination, and reinitiation. The successful accomplishment of each of these steps requires a number of accessory factors. Many of these factors operate at multiple steps in the transcription cycle. The major focus of this study was to examine the function of Clp1, which is an RNA processing factor operating at the 3′ end of genes, in the transcription cycle. Clp1 is one of the …

Single-Stranded Dna Scanning And Deamination With Single-Molecule Resolution, Padhuk Gamarala Senavirathne

Wayne State University Dissertations

SINGLE-STRANDED DNA SCANNING AND DEAMINATION WITH SINGLE-MOLECULE RESOLUTION

by

GAYAN SENAVIRATHNE

December 2013

Advisor: Dr. David Rueda & Dr.Ashok Bhagwat

Major: Chemistry (Analytical)

Degree: Doctor of Philosophy

APOBEC3G (Apo3G) and Activation-Induced cytidine Deaminase (AID) are the most notable members of APOBEC enzymes, a family of cytidine (C) deaminases with crucial functions in the immune system. In T cells, Apo3G deaminates C in viral cDNA to halt the replication of HIV 1 strains lacking viral infectivity factor (vif). In B cells, AID is required for the diversification of antibodies through initiation of somatic hypermutation (SHM), and class switch recombination (CSR), by …

Design, Synthesis, Biological Evaluation And Molecular Modeling Studies Of Novel Multifunctional Neuroprotective Drugs For The Treatment Of Parkinson's Disease: An Effort Towards The Improvement Of In Vivo Efficacy And Modulation Of Alpha Synuclein Aggregation Property Of The Neuroprotective Parent, Gyan Prakash Modi

Wayne State University Dissertations

DESIGN, SYNTHESIS, BIOLOGICAL EVALUATION AND MOLECULAR MODELING STUDIES OF NOVEL MULTIFUNCTIONAL NEUROPROTECTIVE DRUGS FOR THE TREATMENT OF PARKINSON'S DISEASE: AN EFFORT TOWARDS THE IMPROVEMENT OF IN VIVO EFFICACY AND MODULATION OF ALPHA SYNUCLEIN AGGREGATION PROPERTY OF THE NEUROPROTECTIVE PARENT MOLECULE (D-264)

by

GYAN PRAKASH MODI

May 2014

Advisor: Dr. Aloke K. Dutta

Major: Pharmaceutical Sciences

Degree: Doctor of Philosophy

Parkinson's disease (PD) is a progressive age-related neurodegenerative disorder of the central nervous system that is characterized by gradual loss of dopaminergic neurons in the substantia nigra region of the brain. The research from the past two decades in PD area …

The Transcriptional Regulation Of Flagellin-Induced Innate Protection Of The Cornea: Role Of Irf1 And Atf3, Gi Sang Yoon

Wayne State University Dissertations

Pre-exposure of the cornea to TLR5 ligand flagellin induces profound mucosal innate protection against infections by reprogramming gene expression. This study explored the flagellin-induced modifications of transcription factor expression and function, specifically of IRF1 and ATF3 in corneal epithelial cells to elucidate the transcriptional mechanisms underlying the protective function of flagellin on the cornea.

Initially we used Superarray to screen for transcription factors and identified Interferon Regulatory Factor (IRF) 1 and Activating Transcription Factor (ATF) 3 as the most drastically affected genes by flagellin pretreatment in P. aeruginosa challenged human corneal epithelial cells (CEC). However, flagellin pretreatment had opposite effects …

A Relevance Feedback-Based System For Quickly Narrowing Biomedical Literature Search Result, Massuod Hassan Alatrash

Wayne State University Dissertations

The online literature is an important source that helps people find the information. The quick increase of online literature makes the manual search process for the most relevant information a very time-consuming task and leads to sifting through many results to find the relevant ones. The existing search engines and online databases return a list of results that satisfy the user's search criteria. The list is often too long for the user to go through every hit if he/she does not exactly know what he/she wants or/and does not have time to review them one by one. My focus is …

Assessment Of Attention In Vasopressin-Deficient Brattleboro Rats Using A Five-Choice Serial Reaction Time Task, Michael D. Berquist Ii

All NMU Master's Theses

No abstract provided.

Headbanging By Pigeons Iii. A Systematic Replication And Extension Of An Animal Model Of Psychopathology, Darin Allen Casler

All NMU Master's Theses

No abstract provided.

Fluvial And Adfluvial Brook Trout (Salvelinus Fontinalis) Movement Patterns Within Sevenmile Creek And Mosquito River, Pictured Rocks National Lakeshore, Michigan, Robert L. Cross

All NMU Master's Theses

No abstract provided.