Digital Commons Network^™

Synthesis Of A New Scytovirin Protein Derivative, Sd1-Sd2-Sd2, To Decrease Hiv-1 Binding Affinity, Alexandra M. Wood

Honors Theses

Scytovirin is a novel anti-viral protein isolated from the cyanobacterium Scytonema varium. This protein has produced great scientific interest for its anti-human immunodeficiency virus (HIV) activity. Scytovirin disrupts the mechanism of HV infection by binding to HIV-1 proteins glycoprotein 120, glycoprotein 160, and glycoprotein 41. It does not bind to the CD4 receptor on human immune cells. The wild-type protein consists of a single 95 amino acid chain. When folded, Scytovirin's amino acid sequence is arranged into two distinct domains, each with a high degree of sequence conservation. This implicates the importance of both domains in anti-HIV activity. Primers were …

The Inhibition Of Splicing Of Glucose-6-Phosphate Dehydrogenase Mrna During Starvation By Heterogeneous Nuclear Ribonucleoprotein K, Travis Cyphert

Graduate Theses, Dissertations, and Problem Reports (ETD)

Splicing of nascent RNA transcripts is an essential step in gene expression and a target of nutrient regulation. Glucose-6-phosphate dehydrogenase (G6PD) is a lipogenic gene whose expression is regulated exclusively at this posttranscriptional stage. Expression of G6PD mRNA increases 15- to 17-fold during refeeding and is inhibited 80-90% by starvation and the addition of polyunsaturated fat to the diet. The large changes in the accumulation of G6PD mRNA are due to changes the rate of pre-mRNA splicing and not changes in its transcription. In this regard, dietary carbohydrate enhances intron removal, which increases the accumulation of G6PD mRNA. Starvation alters …

Characterizing Runoff Responses In A Mountaintop Mine Impacted And Forested Catchment In The Coalfields Of West Virginia, Andrew J. Miller

Graduate Theses, Dissertations, and Problem Reports (ETD)

Mountaintop mining (MTM) represents the largest land cover/landuse change in the Central Appalachian region. By 2012, the U.S. EPA estimates that MTR will have impacted approximately 6.8% of the predominately forested Appalachian Coalfield region of West Virginia (WV), Kentucky, Tennessee, and Virginia with nearly 4,000 miles of headwater streams buried under valley fills (VF). In spite of the scale and extent of MTM, its hydrologic impacts are poorly understood. Several devastating floods in the region have been attributed to MTM, but there is little evidence to either confirm or refute this belief. Existing research on the hydrologic impacts of MTM …

Patterns Of Lung Cancer Care And Associated Health Outcomes Among Elderly Medicare Fee For Service Beneficiaries In West Virginia And In The United States, Pramit Amrutlal Nadpara

Graduate Theses, Dissertations, and Problem Reports (ETD)

The elderly carry a disproportionate burden of lung cancer in the US. Although significant improvements have been made during the past decade in cancer treatment, substantial disparities still exist in guideline-based lung cancer care and outcomes. Such variation in lung cancer care is a cause for major concern in rural areas like West Virginia (WV). The purpose of this study was to do a comprehensive evaluation of variations in lung cancer care and associated health outcomes in the elderly. This retrospective study was conducted using SEER-Medicare and WVCR--Medicare linked data files for the years 2002-2007. As part of the project, …

Temporal Expression Of Key Angioregulatory Proteins In Response To Exercise And Detraining, Sara A. Olenich

Graduate Theses, Dissertations, and Problem Reports (ETD)

Angiogenesis is an important adaptation to exercise, occurring in response to a multitude of different stimuli including: shear stress, mechanical stretch, ischemia, electrical stimulation, and exercise. Current thinking suggests skeletal muscle angiogenesis is a temporal process controlled by a balance between positive and negative angiogenic proteins. But there is limited information on what molecular mediators control skeletal muscle angiogenesis in this time line, creating a critical need to clarify how individual protein responses regulate physiologic skeletal muscle angiogenesis in response to exercise training and/or physical deconditioning. Our objective is to characterize the temporal expression of several key positive (VEGF, MMP-2, …

Roles Of Speech-Language Pathologists In The Neonatal Intensive Care Unit, Katy Lane Greenlee

Honors Theses

This thesis examined the roles of speech-language pathologists (SLPs) within the Neonatal Intensive Care Unit (NICU). In 2004, the American Speech-Language-Hearing Association published a set of policy documents regarding speech-language pathology practice in the NICU. Research compiled in the literature review explained and supported each of the roles listed in the ASHA policy documents. The literature also revealed that very few sources specific to the speech-language pathology field addressed roles other than those related to feeding and swallowing evaluation and intervention. This research investigated ASHA-certified, masters-level SLPs' familiarity with each of their possible roles in the NICU, as well as …

Skeletal Cell Differentiation Is Enhanced By Atmospheric Dielectric Barrier Discharge Plasma Treatment., Marla J Steinbeck, Natalie Chernets, Jun Zhang, Deepa S Kurpad, Gregory Fridman, Alexander Fridman, Theresa A. Freeman

Department of Orthopaedic Surgery Faculty Papers

Enhancing chondrogenic and osteogenic differentiation is of paramount importance in providing effective regenerative therapies and improving the rate of fracture healing. This study investigated the potential of non-thermal atmospheric dielectric barrier discharge plasma (NT-plasma) to enhance chondrocyte and osteoblast proliferation and differentiation. Although the exact mechanism by which NT-plasma interacts with cells is undefined, it is known that during treatment the atmosphere is ionized generating extracellular reactive oxygen and nitrogen species (ROS and RNS) and an electric field. Appropriate NT-plasma conditions were determined using lactate-dehydrogenase release, flow cytometric live/dead assay, flow cytometric cell cycle analysis, and Western blots to evaluate …

Inhibitory Effect Of Il-17 On Neural Stem Cell Proliferation And Neural Cell Differentiation., Zichen Li, Ke Li, Lin Zhu, Quancheng Kan, Yaping Yan, Priyanka Kumar, Hui Xu, Abdolmohamad Rostami, Guang-Xian Zhang

Department of Neurology Faculty Papers

BACKGROUND: IL-17, a Th17 cell-derived proinflammatory molecule, has been found to play an important role in the pathogenesis of autoimmune diseases, including multiple sclerosis (MS) and its animal model, experimental autoimmune encephalomyelitis (EAE). While IL-17 receptor (IL-17R) is expressed in many immune-related cells, microglia, and astrocytes, it is not known whether IL-17 exerts a direct effect on neural stem cells (NSCs) and oligodendrocytes, thus inducing inflammatory demyelination in the central nervous system.

METHODS: We first detected IL-17 receptor expression in NSCs with immunostaining and real time PCR. We then cultured NSCs with IL-17 and determined NSC proliferation by neurosphere formation …

Inhibitory Effect Of Matrine On Blood-Brain Barrier Disruption For The Treatment Of Experimental Autoimmune Encephalomyelitis., Su Zhang, Quan-Cheng Kan, Yuming Xu, Guang-Xian Zhang, Lin Zhu

Department of Neurology Faculty Papers

Dysfunction of the blood-brain barrier (BBB) is a primary characteristic of experimental autoimmune encephalomyelitis (EAE), an experimental model of multiple sclerosis (MS). Matrine (MAT), a quinolizidine alkaloid derived from the herb Radix Sophorae Flave, has been recently found to suppress clinical EAE and CNS inflammation. However, whether this effect of MAT is through protecting the integrity and function of the BBB is not known. In the present study, we show that MAT treatment had a therapeutic effect comparable to dexamethasone (DEX) in EAE rats, with reduced Evans Blue extravasation, increased expression of collagen IV, the major component of the basement …

3d Micron-Scale Imaging Of The Cortical Bone Canal Network In Human Osteogenesis Imperfecta (Oi), John R. Jameson, Carolyne Albert, Bjoern Busse, Peter A. Smith, Gerald F. Harris

Biomedical Engineering Faculty Research and Publications

Osteogenesis imperfecta (OI) is a genetic disorder leading to increased bone fragility. Recent work has shown that the hierarchical structure of bone plays an important role in determining its mechanical properties and resistance to fracture. The current study represents one of the first attempts to characterize the 3D structure and composition of cortical bone in OI at the micron-scale. A total of 26 pediatric bone fragments from 18 individuals were collected during autopsy (N_c=5) or routing orthopaedic procedures (N_OI=13) and imaged by microtomography with a synchrotron light source (SRµCT) for several microstructural parameters including cortical porosity …

Improving Brain–Machine Interface Performance By Decoding Intended Future Movements, Francis R. Willett, Aaron J. Suminski, Andrew H. Fagg, Nicholas G. Hatsopoulos

Biomedical Engineering Faculty Research and Publications

Objective. A brain–machine interface (BMI) records neural signals in real time from a subject's brain, interprets them as motor commands, and reroutes them to a device such as a robotic arm, so as to restore lost motor function. Our objective here is to improve BMI performance by minimizing the deleterious effects of delay in the BMI control loop. We mitigate the effects of delay by decoding the subject's intended movements a short time lead in the future. Approach. We use the decoded, intended future movements of the subject as the control signal that drives the movement of our BMI. This …

'A Triumph Of Brains Over Brute': Women And Science At The Horticultural College, Swanley, 1890-1910, Donald L. Opitz

School of Continuing and Professional Studies Faculty and Staff Works

The founding of Britain's first horticultural college in 1889 advanced a scientific and coeducational response to three troubling national concerns: a major agricultural depression; the economic distress of single, unemployed women; and imperatives to develop the colonies. Buoyed by the technical instruction and women's movements, the Horticultural College and Produce Company, Limited, at Swanley, Kent, crystallized a transformation in the horticultural profession in which new science-based, formalized study threatened an earlier emphasis on practical apprenticeship training, with the effect of opening male-dominated trades to women practitioners. By 1903, the college closed its doors to male students, and new pathways were …

The Wild Child: Children Are Freaks In Antebellum Novels, Heathe Bernadette Heim

Dissertations, Theses, and Capstone Projects

This dissertation investigates the spectacle of antebellum freak shows and focuses on how Phineas Taylor Barnum's influence permeates five antebellum novels. The study concerns itself with wild children staged as freaks in Margaret by Sylvester Judd, City Crimes by George Thompson, The Scarlet Letter by Nathaniel Hawthorne, Uncle Tom's Cabin by Harriet Beecher Stowe and Our Nig by Harriet Wilson. Barnum's influence was pervasive. The novels I investigate span a period of fourteen years before the Civil War, and offer a view of the kid show presented by the freaks in each text. Touching into spectacle, authors construct narratives …

Dissociation And Potential Space On The Rorschach As Predictors Of Concurrent Ptsd And Substance Dependence Treatment Outcomes, Stephen John Anen

Dissertations, Theses, and Capstone Projects

Both posttraumatic stress disorder (PTSD) and substance use disorders (SUD) are ongoing public health crises. Dissociative experiences are considered core processes within both of these conditions (van der Kolk & van der Hart, 1989; Briere & Runtz, 1987; Schafer et al., 2010). Dissociation, which involves the compartmentalization of psychic experience, also exerts a significant influence over psychotherapies that aim to address both PTSD and SUD (Davidson & Foa, 1991; Spitzer, Barnow, Freyberger, & Grabe, 2007). However, dissociation is a wide concept that encompasses several perceptual, cognitive, affective, memory, and self-state processes (Bernstein & Putnam, 1986; Briere, Weathers, & Runtz, 2005). …

Reflective Functioning And Differentiation-Relatedness During Pregnancy And Infant Attachment Outcomes At One Year, Amy Elizabeth Daley

Dissertations, Theses, and Capstone Projects

This study compared maternal reflective functioning (RF) and differentiation-relatedness (DR) during pregnancy and examined how these processes relate to the quality of mother-infant attachment at one year. The subjects were 35 mother-infant pairs drawn from the control group of a longitudinal treatment study, "Minding the Baby (MTB)," a federally and privately funded home intervention program developed jointly by the Yale School of Nursing and Yale Child Study Center, led by Drs. Lois Sadler and Arietta Slade, and targeting a low socio-economic status area of New Haven, CT. The Pregnancy Interview (Slade, 2003) was administered to the women (ages 14-25 years) …

Children And Elephants: A Study Of Mentalization, Empathy, And Attitudes Towards Conservation In Participants Of An Elephant-Based Environmental Intervention In West Africa, Erica C. Rogers

Dissertations, Theses, and Capstone Projects

Recent research suggests that children's identification with animals and propensity to learn from animal experiences might make animal-focused interventions ideal for social and emotional development. A child's ability to understand their own and others' feeling states has been linked to emotional resilience and has been identified as a protective factor against the development of pathology later in life (Allen & Fonagy, 2006). This study examined the impact of an ongoing conservation-based intervention in Burkina Faso on conservation attitudes, mentalization and empathy. Participants were 106 Burkinabe students, 56 male and 50 female, ages 9-15. Participants were split into four groups, a …

Blogging Chronic Illness And Negotiating Patient-Hood: Online Narratives Of Women With Ms, Collette Sosnowy

Dissertations, Theses, and Capstone Projects

Personal narratives about women's everyday lives with chronic illness are mapped onto the landscape of social media through blogging. Social media is facilitating an already-existing shift in patients' roles as they are increasingly enabled and expected to self-educate themselves about their illness, collaborate with providers, self-manage their care, and engage in health activism. The health care industry has seized on the widespread use of social media to bolster rhetoric that the accelerated knowledge development made possible through social media has the potential to revolutionize the practice of medicine. Critics, however, argue that responsibility and activism via digital technologies has become …

Pathways To High-Lethality Suicide Attempts, Megan Schaffer Chesin

Dissertations, Theses, and Capstone Projects

The purpose of this study was to develop a model of the trajectory to high-lethality suicidal behavior for individuals with Major Depressive Disorder (MDD) and Borderline Personality Disorder (BPD). An increased number of previous suicide attempts, substance use immediately prior to the attempt, and objective planning were proposed to lead directly to an attempt of higher lethality. Meanwhile, aggression and impulsivity were hypothesized to lead indirectly, through their association with past suicidal behavior, to a higher lethality attempt. Path analysis revealed a revised model that applied only to individuals with BPD. In this final model, impulsivity was found to be …

Becoming Someone Different: A Grounded Theory Study Of How Nurses Integrate Pregnancy And Full Time Employment, Paul Gregory Quinn

Dissertations, Theses, and Capstone Projects

In the United States, 40% of the contemporary nursing workforce is comprised of women of childbearing age, 65% of whom are employed full-time. Hence, the likelihood of pregnancy occurring for this population at some point in their employment is high. A holistic exploration of how nurses integrate pregnancy and full-time employment has been lacking. The purpose of this research was to explore how primiparous nurses managed pregnancy and full-time employment. Using a grounded theory approach, nurses who were pregnant and delivered their first baby, while employed full-time on 12-hour work shifts, provided a firsthand account of how they incorporated pregnancy …

Theoretical Methods For Blur-Correction In Electron And Soft X-Ray Microscopy, Joanna Klukowska

Dissertations, Theses, and Capstone Projects

The process of object reconstruction from projections is widely used in many fields. One of the applications is the reconstruction of biological specimens from two-dimensional projections in transmission electron microscopy and transmission x-ray microscopy. Various methods have been developed for correcting the blurring that occurs when the projections are obtained by a real instrument. As the attainable resolution increases, new issues become apparent and need to be taken into account in the imaging model. In this dissertation we concentrate on the point spread function and its impact on the quality and usefulness of the reconstructions from images obtained according to …

The Expression Of The Transcription Factor Broad And Rna-Binding Factors In The Midgut Of The Mosquito Aedes Aegypti During Metamorphosis, Kathryn Ray

Dissertations, Theses, and Capstone Projects

Transcription factors, microRNAs and RNA binding factors frequently interact to coordinate gene expression during development. The transcription factor BROAD (BR) is a global regulator of insect gene transcription and governs the timing of the commitment to pupate. I determined BR expression in the Ae. aegypti midgut by qPCR, and correlated its expression with that of nine miRNAs and three RNA-binding factors. During midgut metamorphosis the expression of these factors was dynamic and reproducible.

To better understand the changes in expression patterns, I evaluated the effects of hormone analogs on expression. Using this approach I uncovered concurrent up-regulation of BR, miR-34 …

Boronic Acids As Penicillinase Inhibitors, Juan F. Barquero

Dissertations, Theses, and Capstone Projects

β-lactamases are enzymes produced by bacteria resistant to antibiotics. A common feature on beta lactam antibiotics is the beta-lactam ring. β-lactamases hydrolyze the β-lactam ring leaving the antibiotic inoperative. The advent of bacteria that are resistant to β-lactams has impelled researchers to find inhibitors for β-lactamases that mimic the lactam ring but do not get hydrolyzed. One group of these new antibiotics is the aryl boronic acids. The main reason the boronic acids have been chosen as potential drugs is their lack of toxicity and their easy excretion in the urine. One of the most important structural features of these …

Polycationic Perylenebisimides For G-Quadruplex Binding, Andrew Jacob Matrick

Honors Theses

Deoxyribonucleic acid (DNA) can exist in a quadruplex arrangement that requires an abundance of guanine residues [2]. The structures of various quadruplexes have been revealed, in vitro, and, more recently, in vivo [4,5]. Guanine quadruplexes are thermo dynamically stable. It is postulated that quadruplex destabilization facilitates a conformational change in the structure that could serve as a switch for gene expression [5]. This principle is the basis for targeting quadruplexes with organic, small-molecule binding and stabilizing agents. Quadruplexes may play an important role in regulating the expression of many genes, including those implicated in cancer. Stabilization of the quadruplex contained …

Geostatistical Analysis Of Arsenic In Soils At The University Of Mississippi Field Station., Elsie Ekene Okoye

Honors Theses

Arsenic is pervasive throughout North Mississippi’s soils (Pettry and Switzer, 2001; Fordham and Norrish, 1983 and; Kaiser and Guggenberger, 2003). This is largely due to the use of pesticides and herbicides in past and present years, as arsenic is a fundamental component in chemicals used for agricultural purposes. Soils contaminated with arsenic present a serious hazard to human health due to the possibility of contaminating surface and groundwater, as well as food through plant uptake of arsenic. Consequently, it is important to understand the process and effects of arsenic absorption in soil. The focus of this research is on the …

An Assessment Of Primary Care Physician Shortages In Mississippi, Megumi Diane Mathis

Honors Theses

According to the Department of Health and Human Services, there are Health Professional Shortage Areas in all states and territories of the United States.' Since fewer students graduating from medical school are choosing primary care," it is imperative that future graduates in the field know which regions are in greatest need of primary care physicians. In a joint effort with the Mississippi Rural Physicians Scholarship Program and the Sally McDonnell Barksdale Honors College, this thesis used data obtained from the Mississippi Department of Health, the United States Census Bureau, the Mississippi State Medical Association, and the Mississippi State Board of …

Shifting Local Ecologies: Biocultural Interactions And Ecuadorian Public Health, Sabrina Bradford

Honors Theses

Though biology is considered universal, this thesis argues against the assumption of the universal applications of biomedicine in favor of a biocultural approach, emphasizing shifting local ecologies in an increasingly globalized world. This research investigated Ecuadorian health within an ecological context in coordination with an international public health internship that was completed through the Universidad San Francisco de Quito and the Ecuadorian Ministerio de Salud Pública. Throughout this thesis, examples are provided demonstrating that globalization, though appearing as a homogenous process on the international level, creates heterogeneous effects on the local scale. To explain the mechanisms and implications of these …

Human Rights In Transit: Nationality, Anti-Haitianism, And The Reactionary State In The Dominican Republic, Ryan Keith Ezelle

Honors Theses

The Dominican Republic and Haiti share a long and troubled history in which race, ethnicity, and culture have been the primary factors in each country’s contrasting national narrative and identity. For many decades Haitians living in the Dominican Republic and their descendants have suffered from lack of recognition from the Dominican government, but since the 1990s this ensuing condition of statelessness has become more widespread and has been greatly aggravated by several actions taken by the Dominican State. In the first decade of the 2000s, the State introduced new laws and policies, including the Law 285 on Immigration, the civil …

Theorizing Factors Influencing Missile Transfers: A Case Study If Sino-Iranian Missile Transfers And Implications For Regional Security, Paul Alexander Kynerd

Honors Theses

Scholars suggest missiles have become the weapon of choice in modem warfare. Missiles are effective against a variety of targets, can travel long distances, and can decrease the need for troops to be engaged in conflict. Nations unable to mobilize large amounts of troops may depend on missiles to equalize military capabilities. Missile stockpiling also serves as a conflict deterrent. What factors influence missile sales between nations and what are the implications of those sales on regional security? This research broadly analyzes the effects of political, socioeconomic, conflict, temporal, and regional factors on global missile sales between 1980 and 2009, …

Attitudes Towards Homosexuality In China, Mallory Elaine Lee

Honors Theses

Since Mao Zedong’s rise to power in China, homosexuality is has been a relatively under-studied topic. In order to identify factors that influence both self-identification of homosexuality and attitudes towards homosexuality, both qualitative and quantitative analysis was utilized. While the data analysis found that the variables were not significant in relation to one another, it is obvious that various factors may influence both a person’s willingness to come out as homosexual and the attitudes of Chinese people towards homosexual. While these conclusions are not definitive, this thesis opens the door for further research into attitudes towards homosexuality and self-identification of …

Sheparding Gender: Queering Video Games In The Mass Effect Series, Karina Rene Popp

Honors Theses

The Mass Effect series is discussed as a case study for how video games construct gendered aspects of their lore and gameplay in order to appease what developers believe, and most likely is, their consumer base. While queering is possible both for the characters in the game and the gameplay as a performance it is also limited by appeasing an implied straight, male gamer.