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2013

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Wound Healing Potential Of Copper (Ii) Bis [N'- (5-Chloro-1hindol-3-Yl) Methyl Nicotinic Hydrazide] On Experimentally Induced Excision Wounds In Rats / Morvarid Rahnama, Morvarid Rahnama Jan 2013

Wound Healing Potential Of Copper (Ii) Bis [N'- (5-Chloro-1hindol-3-Yl) Methyl Nicotinic Hydrazide] On Experimentally Induced Excision Wounds In Rats / Morvarid Rahnama, Morvarid Rahnama

Student Works (2010-2019)

Considering the importance of wounds in human health proclaims the necessity of discovering and developing new effective drug-to-be agents. Cl-indole-nicotinic is a Schiff base compound with promising structural potentials in accelerating healing of wounds. The purpose of present study is to investigate Cl-indole-nicotinic effects on wound healing and acute toxicity on Sprague Dawley rats as well as its activity as an antimicrobial and an antioxidant agent. The manually induced dermal incision on dorsal neck area of four groups of male SD rats were treated for 10 days with high dose compound (50 mg/kg), low dose compound (25 mg/kg), CMC 5%, …

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Method Validation For The Determination Of Hexavalent Chromium In Seawater And Other Matrices By Colorimetric Method / Yong Pui San, Pui San Yong Jan 2013

Method Validation For The Determination Of Hexavalent Chromium In Seawater And Other Matrices By Colorimetric Method / Yong Pui San, Pui San Yong

Student Works (2010-2019)

The research project is about the method validation for the determination of hexavalent chromium in seawater and other matrices by colorimetric method. This method is validated by referring to the APHA 3500 B- Cr Colorimetric Method. The current colorimetric method of determination of hexavalent chromium in wastewater is carried out by using UV-VIS Spectrocopy. This method is developed to determine the trace level of hexavalent chromium up to a minimum 0.001 mg /L or 1 ppb of hexavalent chromium in seawater, river water and industrial effluent by using the Discrete Analyzer. The calibration range is within 0 to 0.25 mg …

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Effects And Control Of Barnacle Infestation On Mangrove Seedlings In A Mangrove Rehabilitation Site / Tan Yee Siang, Yee Siang Tan Jan 2013

Effects And Control Of Barnacle Infestation On Mangrove Seedlings In A Mangrove Rehabilitation Site / Tan Yee Siang, Yee Siang Tan

Student Works (2010-2019)

A study on the development, effects and control of biofouling on replanted mangrove seedlings (40-50cm height) was carried out, from April 2008 to July 2010, at Kampung Sungai Haji Dorani and Kampung Sungai Limau, Selangor, Malaysia. The dominant biofoulers were barnacles (Amphibalanus amphitrite), which were the main causal organism of mangrove seedling damage or death. The high density (>100 no./ 10 cm section of plant stem) and cover (>30 %) of barnacles appeared to obstruct development of new leaves, shoots and roots, as well as imposing weight loads as high as six times the weight of the seedlings. …

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Phylogenetics Of Bactrocera Fruit Flies In Malaysia With Reference To Selected Host Fruits / Joey Ee Uli, Ee Uli Joey Jan 2013

Phylogenetics Of Bactrocera Fruit Flies In Malaysia With Reference To Selected Host Fruits / Joey Ee Uli, Ee Uli Joey

Student Works (2010-2019)

Bactrocera carambolae and Bactrocera papayae fruit flies, also known as the carambola and papaya fruit fly respectively, are significant pest fruit flies in Malaysia. They are capable of causing losses in the agricultural industry by infesting host fruits and making them unable to be sold. These two species are grouped in the Bactrocera dorsalis species complex, and are oftentimes difficult to distinguish from one another due to similar and intermediate morphological characteristics. A precise method for identifying pest fruit flies is important to properly monitor the infestation of host fruits, for the purpose of quarantine management. The aims of this …

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Cloning, Expression And Functional Study Of Specific Dna Markers For Salmonella Typhi / Noradilin Binti Abdullah, Noradilin Abdullah Jan 2013

Cloning, Expression And Functional Study Of Specific Dna Markers For Salmonella Typhi / Noradilin Binti Abdullah, Noradilin Abdullah

Student Works (2010-2019)

ST332 is a 332 bp gene marker used in a patented Salmonella kit for detection of foodborne pathogen, Salmonella Typhi. As the gene is specific in DNA based detection by using polymerase chain reaction (PCR), its specificity in immunoassay is studied. For this purpose, an expression system of the gene is constructed and the expressed recombinant ST332 protein is tested for reactivity by hybridizing it towards typhoid patients sera through dot blotting. Initially, the DNA fragment was amplified with PCR and cloned into pGEX-4T-1 plasmid vector and transformed into E. coli BL21. Sequencing of the 332 bp fragment identified it …

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Nationwide Distribution And Insecticide Resistance Study Of Malaysian Mosquito Culex Quinquefasciatus Say By Molecular And Biochemical Tools / Low Van Lun, Van Lun Low Jan 2013

Nationwide Distribution And Insecticide Resistance Study Of Malaysian Mosquito Culex Quinquefasciatus Say By Molecular And Biochemical Tools / Low Van Lun, Van Lun Low

Student Works (2010-2019)

A nationwide investigation was carried out to (1) determine the distribution of Culex quinquefasciatus and other species of mosquitoes in stagnant water in residential areas, (2) investigate the genetic diversity of Cx. quinquefasciatus, (3) quantify the insecticide susceptibility status of Cx. quinquefasciatus, (4) characterize the biochemical mechanisms of insecticide resistance in Cx. quinquefasciatus, (5) characterize the molecular mechanisms of insecticide resistance in Cx. quinquefasciatus from 13 states and one federal territory in Malaysia. Culex larval surveillance indicated that Cx. quinquefasciatus was the predominant species in residential areas. Several habitat characteristics (i.e., pH, conductivity, salinity, total dissolved solids, elevation and dissolved …

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Isolation And Characterization Of Listeria Monocytogenes From Ready-To-Eat Food / Hossein Jamali, Hossein Jamali Jan 2013

Isolation And Characterization Of Listeria Monocytogenes From Ready-To-Eat Food / Hossein Jamali, Hossein Jamali

Student Works (2010-2019)

Listeria monocytogenes (L. monocytogenes) is an important cause of listeriosis, a common foodborne disease of public health importance. Ready-to-eat (RTE) foods are known as a potential source of listeriosis. There are limited studies in the characterisation of L. monocytogenes in Malaysia. The objectives of the study were to determinate the prevalence of Listeria species and L. monocytogenes in RTE foods in Malaysia and to characterise these isolates by using molecular methods. Two hundred fifty RTE food samples, including cooked beef and beef products, beverages, cooked chicken and chicken products, fried egg and egg products, packed lunch, salad and vegetables, and …

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Etika Dalam Bioteknologi Moden : Kajian Ke Atas Respons Para Ilmuan Islam Terpilih Mengenai Garis Panduan Etika Islam / Noor Munirah Binti Isa, Noor Munirah Isa Jan 2013

Etika Dalam Bioteknologi Moden : Kajian Ke Atas Respons Para Ilmuan Islam Terpilih Mengenai Garis Panduan Etika Islam / Noor Munirah Binti Isa, Noor Munirah Isa

Student Works (2010-2019)

Perkembangan bioteknologi moden pada hari ini berpotensi untuk mendatangkan banyak kebaikan, namun pada masa yang sama terdapat beberapa aplikasi bioteknologi moden yang menimbulkan keraguan dalam masyarakat mengenai keselarasannya dengan ajaran agama. Selain itu timbul persoalan-persoalan mengenai pendekatan yang paling sesuai untuk mengkaji aspek etika aplikasi-aplikasi tersebut. Dengan memberi tumpuan kepada panduan etika Islam, kajian ini bertujuan untuk mengkaji respons para ilmuan Islam terpilih dalam menghadapi cabaran-cabaran ini. Aplikasi-aplikasi bioteknologi moden yang dikaji ialah aplikasi pengklonan manusia bagi tujuan pembiakan, penggunaan sel stem bagi tujuan perubatan dan makanan yang diubahsuai secara genetik. Secara khususnya objektif-objektif kajian ini ialah: a) mengkaji mekanisme, …

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Some Outlier Problems In A Circular Regression Model / Safwati Binti Ibrahim, Safwati Ibrahim Jan 2013

Some Outlier Problems In A Circular Regression Model / Safwati Binti Ibrahim, Safwati Ibrahim

Student Works (2010-2019)

This study looks at three problems related to the JS circular regression model with five objectives in mind. The first two objectives are concerned with the problem of outliers in the model. The first is the investigation of the robustness of the JS circular regression estimation method in the presence of outliers in the data set. The second is the use of three numerical tests based on row deletion approach to detect possible outliers in the JS circular regression model. The first test considered is a modified version of the COVRATIO statistic by utilizing the covariance matrix of residuals of …

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Isolation, Purification And Antimicrobial Action Of Peptides Produced By Streptococcus Salivarius Isolated From Malaysian Subjects / Abdelahhad Barbour, Abdelahhad Barbour Jan 2013

Isolation, Purification And Antimicrobial Action Of Peptides Produced By Streptococcus Salivarius Isolated From Malaysian Subjects / Abdelahhad Barbour, Abdelahhad Barbour

Student Works (2010-2019)

Streptococcus salivarius is known to produce different bacteriocin-like inhibitory substances (BLIS). In this study six S. salivarius strains were isolated from different Malaysian subjects. The isolates were identified using morphological and biochemical characterization and 16S rDNA gene sequencing. Levansucrase enzyme was characterized from sucrose-enriched culture of strain S. salivarius YU10. BLIS production was investigated using simultaneous and deferred antagonism tests that showed YU10 and NU10 as the best BLIS-producing strains among the Malaysian isolates in this study. Strain HJEFF was the only S. salivarius isolate which produced a more limited quantity of BLIS in liquid medium. However, all other strains …

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Optical Properties And Mathematical Model For Water Based Alumina And Titania Nanofluids / Mohammad Sajid Hossain, Mohammad Sajid Hossain Jan 2013

Optical Properties And Mathematical Model For Water Based Alumina And Titania Nanofluids / Mohammad Sajid Hossain, Mohammad Sajid Hossain

Student Works (2010-2019)

The purpose of this research is to experimentally investigate optical behaviour of Alumina and Titania nanofluids. Effects of aggregation on optical properties along with development of a mathematical model for extinction coefficient have also been done. In this dissertation, Classical theories such as, Rayleigh, Quasi Crystalline, Maxwell-Garnett and Lambert-Beer’s approaches are used for analytical analysis. Experiment is conducted for 0.03, 0.05 and 0.08 %v/v concentrations at different time intervals. Outcome of the dependencies are then combined together to obtain a realistic mathematical model for measurement of extinction coefficient. Results of the study show that Alumina nanofluids are very stable for …

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Optical Properties And Mathematical Model For Water Based Alumina & Titania Nanofluids / Mohammad Sajid Hossain, Mohammad Sajid Hossain Jan 2013

Optical Properties And Mathematical Model For Water Based Alumina & Titania Nanofluids / Mohammad Sajid Hossain, Mohammad Sajid Hossain

Student Works (2010-2019)

The purpose of this research is to experimentally investigate optical behaviour of Alumina and Titania nanofluids. Effects of aggregation on optical properties along with development of a mathematical model for extinction coefficient have also been done. In this dissertation, Classical theories such as, Rayleigh, Quasi Crystalline, Maxwell-Garnett and Lambert-Beer’s approaches are used for analytical analysis. Experiment is conducted for 0.03, 0.05 and 0.08 %v/v concentrations at different time intervals. Outcome of the dependencies are then combined together to obtain a realistic mathematical model for measurement of extinction coefficient. Results of the study show that Alumina nanofluids are very stable for …

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Anti-Quorum Sensing Properties Of Melicope Lunu-Ankenda, Piper Betle, Gnetum Gnemon And Piper Nigrum / Tan Li Ying, Li Ying Tan Jan 2013

Anti-Quorum Sensing Properties Of Melicope Lunu-Ankenda, Piper Betle, Gnetum Gnemon And Piper Nigrum / Tan Li Ying, Li Ying Tan

Student Works (2010-2019)

Quorum sensing (QS) is a communication tool of bacteria that is involved in the regulation of bacterial virulence determinants, therefore making it an interesting target to attenuate pathogens. In this study, the anti-QS activities of four Malaysia‟s edible, local plants, namely, Melicopelunu-ankenda, Piper betle, Gnetumgnemon and Piper nigrum were explored by testing the hexane, chloroform and methanol extracts of the plants on Chromobacteriumviolaceum CV026, Escherichia coli [pSB401], E. coli [pSB1075],Pseudomonas aeruginosa PA01 and P. aeruginosaPA01lecA::lux. Qualitative studies of violacein inhibition of C. violaceum CV026 showed that most of the extracts except for the methanol extract of P. betle and chloroform …

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The Use Of On-Line Analytical Processing Approach In Developing Host-Parasite Database System / Nur Imtiazah Bt. Shuhaimi, Shuhaimi Nur Imtiazah Jan 2013

The Use Of On-Line Analytical Processing Approach In Developing Host-Parasite Database System / Nur Imtiazah Bt. Shuhaimi, Shuhaimi Nur Imtiazah

Student Works (2010-2019)

Biodiversity is a very broad area to explore. The developments of research have resulted in increasing the number of biodiversity data in various formats. The ability of a database in dealing with biodiversity data is essential for the effective management and information dissemination process. The capabilities of a database system rely on the formula or approaches used in operating the system. The development of On-Line Analytical Processing (OLAP) technology from time to time expands the use of this approach which previously was dominated by the business field. The effectiveness of structuring data into cube form technique allows data modeling and …

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Quorum Sensing And Quorum Quenching Abilities Of Selected Bacteria Isolated From Antarctic / See-Too Wah Seng, Wah Seng See-Too Jan 2013

Quorum Sensing And Quorum Quenching Abilities Of Selected Bacteria Isolated From Antarctic / See-Too Wah Seng, Wah Seng See-Too

Student Works (2010-2019)

Quorum sensing (QS) is a phenomenon which refers to bacterial cell-to-cell communication in a population manner. QS regulates diverse activities in bacteria. Bacteria that possess QS activity communicate through secreted signal molecules or autoinducer. The Gram-negative bacteria and archaeon use acyl homoserine lactones (AHLs) as a QS signal to achieve regulation through the luxI-luxR homolog system, wheareas Gram-positive bacteria use small peptides as autoinducers. Some of the Proteobacteria use quinolone compound as signaling molecules. Quorum quenching (QQ) on the other hand refers to interference of QS that inactivates the signaling molecules. Even though QS and QQ were extensively studied in …

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Chemical Recycling Of Waste Pet Into Polyester Polyol For The Production Of Semi-Rigid Pu Foams / Asmalina Mohd Darus, Asmalina Mohd Darus Jan 2013

Chemical Recycling Of Waste Pet Into Polyester Polyol For The Production Of Semi-Rigid Pu Foams / Asmalina Mohd Darus, Asmalina Mohd Darus

Student Works (2010-2019)

One of the biggest problems caused by soft drink poly(ethylene terephthalate) PET bottles is the waste problem resulting in environmental pollution. To economically reduce this waste, this study presents a method to recycle the PET bottle by glycolysis and convert it into a polyester polyol that can be used to make polyurethane foam. Common soft-drink PET bottles were glycolysed by glycerol in the presence of zinc acetate as a catalyst. Dependence on glycolysis conversion on reaction temperature and the Arrhenius plot, activation energy obtained of 36.5 kJ/mol. The new polyester polyols namely glycolysed PET have hydroxyl value of 500-800 mg …

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Stability And Rheological Behaviour Of Functional Essential Oils In Glycolipid Cream Emulsion / Saw Mei Mei, Mei Mei Saw Jan 2013

Stability And Rheological Behaviour Of Functional Essential Oils In Glycolipid Cream Emulsion / Saw Mei Mei, Mei Mei Saw

Student Works (2010-2019)

Essential oils carries distinctively unique aroma from parts of a plant where they are extracted. Essential oils have widely been known for its aromatherapy and therapeutic effect on human. High concentration of essential oil comprises of thyme, lavender, tea tree and citronella oil have been induced in emulsions system using olive oil as carrier oil stabilized by glycolipid surfactants of sucrose stearate, surcrose palmitate and sucrose myristate respectively. Co-surfactants, TritonTM X-100 and TritonTM X-45 was utilised to create high interactions and compatibility associated with short chain terpenes, terpenoids and phenolic compound presents in essential oils inverting emulsion droplet forming o/w …

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Development Of Environmental Safety And Health Conceptual Model For Steel Factory / Nur Shuhada Bt. Mohamed Jaafar, Mohamed Jaafar Nur Shuhada Jan 2013

Development Of Environmental Safety And Health Conceptual Model For Steel Factory / Nur Shuhada Bt. Mohamed Jaafar, Mohamed Jaafar Nur Shuhada

Student Works (2010-2019)

Since modernization and industrialization have led to human loss and tragedies, due to industrial accidents all over the world, it is significant to look at the aspects that lead to success of environmental safety and health (ESH) situation. The safety statistics indicate high fatality and injury rates among the manufacturing employees. This paper focuses on developing an ESH Conceptual Model, based on related recommended OSHMS models in the existing of OHSAS: 18001 Standard and Guidelines. Besides, the effectiveness of OSHMS implementation was evaluated, by identifying the potential hazards and practical methods in minimizing workplace accidents. A case study has been …

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Development Of Rapid Lamp Assays For The Detection Of Potential Nosocomial Pathogens / Dong Hong, Hong Dong Jan 2013

Development Of Rapid Lamp Assays For The Detection Of Potential Nosocomial Pathogens / Dong Hong, Hong Dong

Student Works (2010-2019)

Nosocomial pathogens are organisms that cause diseases in a patient during his/her stay in a hospital or health care center. These pathogens can spread easily in the hospital and cause an outbreak because of the low immune system of the hospitalized patients. Even so, they are resistant to most of the antibiotics. Rapid detection of these pathogens would be useful to trace the source of an outbreak. In this study, a new detection method, the loop-mediated isothermal amplification (LAMP) was developed for the rapid detection of three nosocomial pathogens which are highly multidrug resistant. They are Acinetobacter baumannii, Pseudomonas aeruginosa …

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Molecular Studies Of Kappaphycus Doty And Eucheuma J. Agardh: Phylogenetics And Dna Barcode Assessment / Tan Ji, Ji Tan Jan 2013

Molecular Studies Of Kappaphycus Doty And Eucheuma J. Agardh: Phylogenetics And Dna Barcode Assessment / Tan Ji, Ji Tan

Student Works (2010-2019)

Kappaphycus and Eucheuma are the main producers of carrageenan worldwide, with stable annual production increments to cater to increasing demands. Extensively used in the food and cosmetics industries, the marketing of carrageenan generates lucrative returns to the industry and economy. The carrageenan industry is one of the key economic sectors in Malaysia, which also offers a means of livelihood to the local community. The extensive morphological variations of Kappaphycus and Eucheuma often resulted in faming of mixed populations which reduced overall carrageenan yields. Molecular taxonomy is thus applied to identify the many locally-named varieties of Kappaphycus and Eucheuma as well …

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An Ecological Study Of Pteroma Pendula (Iepidoptera: Psychidae) In Oil Palm Plantations With Emphasis On The Predatory Activities Of Oecophylla Smaragdina (Hymenoptera: Formicidae) On The Bagworm / Exelis Moise Pierre, Moise Pierre Exelis Jan 2013

An Ecological Study Of Pteroma Pendula (Iepidoptera: Psychidae) In Oil Palm Plantations With Emphasis On The Predatory Activities Of Oecophylla Smaragdina (Hymenoptera: Formicidae) On The Bagworm / Exelis Moise Pierre, Moise Pierre Exelis

Student Works (2010-2019)

A study entitled “An ecological study of Pteroma pendula (Lepidoptera: Psychidae) in oil palm plantations with emphasis on the predatory activities of Oecophylla smaragdina (Hymenoptera: Formicidae) on the bagworm was conducted at the MPOB Research Plantation at Teluk Intan, Perak, Malaysia from 11.11.2010 to 31.01.2013. The main objectives of the study was to assess the infestation of P. pendula and investigate a correlation between pest density fluctuations with abiotic factors in the study area and to elucidate O. smaragdina occupancy pattern and its predatory behaviours towards P. pendula as well as assessing the productivity of palm trees occupied and not …

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Influence Of Allium Sativum Or Cinnamomum Verum On Fermentation Of Milk And Their Effects On The Physicochemical, Biochemical, Microbiological And Functional Properties Of Probiotic Yogurt / Shori, Amal Bakr, Amal Bakr Shori Jan 2013

Influence Of Allium Sativum Or Cinnamomum Verum On Fermentation Of Milk And Their Effects On The Physicochemical, Biochemical, Microbiological And Functional Properties Of Probiotic Yogurt / Shori, Amal Bakr, Amal Bakr Shori

Student Works (2010-2019)

The present study investigated the effects of Allium sativum or Cinnamomum verum water extract on fermentation of milk and subsequent changes in yogurt bacteria growth and fermentation products on the organoleptic, nutritional and functional values of yogurt. Three types of milk (cow, camel and goat) were incubated (41°C) with starter culture in the presence of A. sativum or C. verum water extract until pH of yogurt was reduced to 4.5. The presence of A. sativum or C. verum water extract in cow, camel and goat milk did not affect pH reduction during fermentation whereas titratable acidity (TA) increased only in …

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E-Waste Management In Selected Institutions Of Higher Learning In The Klang Valley, Malaysia / Pearson Kasapo, Pearson Kasapo Jan 2013

E-Waste Management In Selected Institutions Of Higher Learning In The Klang Valley, Malaysia / Pearson Kasapo, Pearson Kasapo

Student Works (2010-2019)

“E-waste” is the term popularly used to refer to end-of-life or discarded electrical and electronic equipment. Institutions of higher learning (universities) contribute significantly to the rapidly growing threat of E-waste. This research seeks to establish if there are any institutional mechanisms for E-waste management and assess the level of university public (staff and student) knowledge on E-waste. The research focused mainly on Information and Communication Technology (ICT) E-waste management in selected universities in the Klang Valley of Central Region, Malaysia. ICT equipment (computers, printers, copiers) are the most widely used and frequently replaced electronics in universities and if not soundly …

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Using Daisy (Digital Automated Identification System) For Automated Identification Of Moths Of The Superfamily Bombycoidea Of Borneo / Leong Yuen Munn, Yuen Munn Leong Jan 2013

Using Daisy (Digital Automated Identification System) For Automated Identification Of Moths Of The Superfamily Bombycoidea Of Borneo / Leong Yuen Munn, Yuen Munn Leong

Student Works (2010-2019)

The health of our environment can be measured by monitoring moths. Moths are especially useful as biological indicators given that they are sensitive to environmental changes, widespread and can be found in various habitats. Therefore, by monitoring their ranges and numbers, we can obtain essential clues about our changing environment such as climate change, air pollution and the effects of new farming practices. In this study, I tested DAISY as a tool for automated identification of moths. Images of 210 species of the superfamily Bombycoidea from the book “The Moths of Borneo: Part 3: Lasiocampidae, Eupterotidae, Bombycidae, Brahmaeidae, Saturniidae, Sphingidae” …

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Development Of Biological Agents Using Chrysanthemum Indicum (Asteraceae) And Alpinia Galanga (Zingiberaceae) Against Coptotermes Gestroi, Coptotermes Curvignathus And Macrotermes Carbonarius / Partiban S/O Subramanian, Partiban Subramanian Jan 2013

Development Of Biological Agents Using Chrysanthemum Indicum (Asteraceae) And Alpinia Galanga (Zingiberaceae) Against Coptotermes Gestroi, Coptotermes Curvignathus And Macrotermes Carbonarius / Partiban S/O Subramanian, Partiban Subramanian

Student Works (2010-2019)

The antifeedant effects studies of Alpinia galanga rhizome and Chrysanthemum indicum on Coptotermes gestroi, Coptotermus curvignathus and Macrotermus carbonarius were conducted in the laboratory. Dual choice bioassays were conducted using methanolic extract and essential oil of A. galanga. Two paper discs (4.0 cm diameter) were placed in Petri dishes (9 cm diameter). One disc treated with A. galanga or C. indicum extract and another disc was treated with solvent hexane or methanol as control. Ten termites were placed in the treated petri dish. Percentage of antifeedant was determined by calculating the difference in the paper consumption of treated and control. …

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Chemical Constituents And Bioactivity Of Mitrella Kentii (Bl.) Miq. (Annonaceae) / Ainnul Hamidah Syahadah Binti Azizan, Ainnul Hamidah Syahadah Azizan Jan 2013

Chemical Constituents And Bioactivity Of Mitrella Kentii (Bl.) Miq. (Annonaceae) / Ainnul Hamidah Syahadah Binti Azizan, Ainnul Hamidah Syahadah Azizan

Student Works (2010-2019)

The phytochemical and bioactivity studies on Mitrella kentii species from Annonaceae family have been performed. The extraction of phytochemicals from dried and ground plants were performed using conventional method extractions; Soxhlet extractor and Cold-extraction. The isolation of pure compounds from crude extracts were carried out by using various chromatographic methods such as column chromatography (CC), thin layer chromatography (TLC) and preparative thin layer chromatography (PTLC). The structural elucidations of the isolated compounds were established based on the spectroscopic techniques such as UV-Vis, IR, MS, 1D-NMR (1H-NMR and 13C-NMR), 2D-NMR (COSY, DEPT, HSQC and HMBC) and single crystal X-ray diffraction analysis. …

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Overexpression Of Wild-Type Anxa7 Tumor Suppressor Gene Alters Cancer-Related Micrornas In Human Prostate Cancer Cells / Yap Lim Hui, Lim Hui Yap Jan 2013

Overexpression Of Wild-Type Anxa7 Tumor Suppressor Gene Alters Cancer-Related Micrornas In Human Prostate Cancer Cells / Yap Lim Hui, Lim Hui Yap

Student Works (2010-2019)

MicroRNAs (miRNAs) are endogenous 18-25nt RNAs that regulate genes at the protein translation level. They can act as tumor suppressors or oncogene and are often dysregulated in various cancers. ANXA7 is a tumor suppressor gene that encodes for a Ca2+-dependent membrane-binding protein and its expression was found to be reduced or lost in advanced androgen-independent prostate cancers. This research aims to identify miRNAs that are regulated by the ANXA7 tumor suppressor gene and the putative pathways involved in prostate cancer. The overexpression of wild-type ANXA7 was achieved by using a mammalian expression vector harboring the gene. From our miRNA microarray …

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Leachate Toxicity Assessment And Bioremediation Of Leachate Contaminated Soil / Emenike Chijioke Uche, Emenike Chijioke Uche Jan 2013

Leachate Toxicity Assessment And Bioremediation Of Leachate Contaminated Soil / Emenike Chijioke Uche, Emenike Chijioke Uche

Student Works (2010-2019)

Chemical evaluations and characterization had often served as the commonly adopted options for assessing the potential impact of pollutants, which at the same time provide insight into the possible remediation technologies. However, heterogeneous substances may not be best studied in aforementioned forms because of the varied characteristics and concentrations of the discrete components. Leachate which is the liquid by-product of waste decomposition in landfills or dump sites is one such heterogeneous fluid that can easily pollute the environment (terrestrial and aquatic). With increase in waste generation in Malaysia, leachate production becomes inevitable especially as landfilling is the ultimate predominant waste …

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Morphological And Molecular Characterisation Of Monogeneans / Tan Wooi Boon, Wooi Boon Tan Jan 2013

Morphological And Molecular Characterisation Of Monogeneans / Tan Wooi Boon, Wooi Boon Tan

Student Works (2010-2019)

Morphologies and morphometries as diagnostic characters are shown statistically to be able to differentiate between species and intraspecific variants. Intraspecific variants or morphovariants exist in nature but the variations they possessed are not enough to consider them as species (differentiation index values for morphovariants is 50% less than the values for species). These variations are due to genetic difference resulting from cross-fertilisations between different individuals of the same species. Such variations are necessary tools for future species diversification. The 28S rDNA are able to group members of the major dactylogyridean families together showing the monophyly of these families within the …

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Tissue Culture Studies, Secondary Metabolites And Pigment Extraction From Allamanda Cathartica L. / Wong Kim Fah, Kim Fah Wong Jan 2013

Tissue Culture Studies, Secondary Metabolites And Pigment Extraction From Allamanda Cathartica L. / Wong Kim Fah, Kim Fah Wong

Student Works (2010-2019)

Allamanda cathartica L. is a potential medicinal plant which starts to achieve public awareness due to its nutritional value that able to treat various types of diseases, especially in the treatment of jaundice, malaria and cancer. Tissue culture studies were undertaken to examine the effects of different plant growth regulators (PGRs) on the callus induction from leaf and stem explants of this species. Surface sterilization by mercuric (II) chloride (HgCl2) was utilized in order to surface sterilize the leaf (0.1%) and stem (0.2%) explants. The leaf and stem explants were cultured on full-strength Murashige and Skoog (MS) medium supplemented with …

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