Digital Commons Network^™

Exosomes Derived From Hiv-1-Infected Cells Contain Trans-Activation Response Element Rna., Aarthi Narayanan, Sergey Iordanskiy, Ravi Das, Rachel Van Duyne, Steven Santos, Elizabeth Jaworski, Irene Guendel, Gavin Sampey, Elizabeth Dalby, Maria Iglesias-Ussel, Anastas Popratiloff, Ramin Hakami, Kylene Kehn-Hall, Mary Young, Caroline Subra, Caroline Gilbert, Charles Bailey, Fabio Romerio, Fatah Kashanchi

Microbiology, Immunology, and Tropical Medicine Faculty Publications

Exosomes are nano-sized vesicles produced by healthy and virus-infected cells. Exosomes derived from infected cells have been shown to contain viral microRNAs (miRNAs). HIV-1 encodes its own miRNAs that regulate viral and host gene expression. The most abundant HIV-1-derived miRNA, first reported by us and later by others using deep sequencing, is the trans-activation response element (TAR) miRNA. In this study, we demonstrate the presence of TAR RNA in exosomes from cell culture supernatants of HIV-1-infected cells and patient sera. TAR miRNA was not in Ago2 complexes outside the exosomes but enclosed within the exosomes. We detected the host miRNA …

Spatially Explicit Modeling Of Schistosomiasis Risk In Eastern China Based On A Synthesis Of Epidemiological, Environmental, And Intermediate Host Genetic Data, Matthias Schrader, Torsten Hauffe, Zhijie Zhang, George M. Davis, Fred Jopp, Justin V. Remais, Thomas Wilke

Microbiology, Immunology, and Tropical Medicine Faculty Publications

Schistosomiasis japonica is a major parasitic disease threatening millions of people in China. Though overall prevalence was greatly reduced during the second half of the past century, continued persistence in some areas and cases of re-emergence in others remain major concerns. As many regions in China are approaching disease elimination, obtaining quantitative data on Schistosoma japonicum parasites is increasingly difficult. This study examines the distribution of schistosomiasis in eastern China, taking advantage of the fact that the single intermediate host serves as a major transmission bottleneck. Epidemiological, population-genetic and high-resolution ecological data are combined to construct a predictive model capable …

Preventative Care And Culture, Anna Wagman

Independent Study Project (ISP) Collection

This paper explores the intersection between culture and health. The similarities and differences between methods for preventative medicine employed by traditional and allopathic systems are discussed. These ostensibly disparate systems, in reality, display many parallels in regards to their delivery. The effects of industrial progress, and therefore cultural change, on the health of the inhabitants of rapidly urbanizing African cities are examined. Historical information from studies of Great Britain, the first area to undergo a full industrial revolution is also drawn on. These analyses essentially predict the societal issues that come with urbanization, such as the amalgamation of preexisting pollutants …

Vol. 38, No. 2: Summer 2013, School Of Medicine & Health Sciences

North Dakota Medicine Archive

No abstract provided.

Wsu Research News, Spring/Summer 2013, Office Of Research And Sponsored Programs, Wright State University

WSU Research News

A twenty page newsletter of the WSU Research News. The WSU Research News was published monthly beginning in June of 1968 and issued by the Office of Research Development. This newsletter was created to provide information to the WSU faculty about the availability of outside funds for research and educational programs, new developments that may affect availability of funds, and general information on research and educational activities at Wright State University.

Liberalis, Summer 2013, Utah State University

Liberalis

Freedom to Think, Discover, and Create. The alumni magazine for the College of Humanities and Social Sciences at Utah State University.

An Hsus Report: Industrial Farm Animal Production And Livestock Associated Mrsa (Methicillin Resistant Staphylococcus Aureus), The Humane Society Of The United States

Impact of Animal Agriculture

Staphylococcus aureus is a leading cause of bacterial infection and is increasingly found to be resistant to antibiotic therapy. A newly described type of Methicillin Resistant Staphylococcus aureus carried by farm animals, Livestock Associated MRSA (LA-MRSA), is now causing infections in humans with and without direct livestock contact. A reduction in the non-therapeutic use of antibiotics in feed would likely reduce the capacity of industrial animal agriculture to continue to create, disseminate, and perpetuate a large reservoir of LA-MRSA on a global scale, but more fundamental changes in the way animals are raised for food may be necessary forestall a …

Industrial Farm Animal Production And Livestock Associated Mrsa (Methicillin Resistant Staphylococcus Aureus)

Agribusiness Reports

Staphylococcus aureus is a leading cause of bacterial infection and is increasingly found to be resistant to antibiotic therapy. A newly described type of Methicillin Resistant Staphylococcus aureus carried by farm animals, Livestock Associated MRSA (LA-MRSA), is now causing infections in humans with and without direct livestock contact. A reduction in the non-therapeutic use of antibiotics in feed would likely reduce the capacity of industrial animal agriculture to continue to create, disseminate, and perpetuate a large reservoir of LA-MRSA on a global scale, but more fundamental changes in the way animals are raised for food may be necessary forestall a …

The College Of Pharmacy, South Dakota State University

Jackrabbits Script and Scope (Formerly called College of Pharmacy and Allied Health Professions Magazine)

[Page] 2 Endowed professorship created A trust created by a pharmacist who never attended college is providing the funds for the college’s first endowed professorship.
[Page] 3 Berry power The inaugural speaker at the Francis Miller Lecture extols the value of blueberries in fighting cancer — and he’s got the research to prove it. [Page] 4 Medication safety Deidra Van Gilder and Tasha Rausch are part of a national effort to curb medication mistakes through the Patient and Clinical Pharmacy Service Collaborative.
[Page] 6 Ashley Potter The P3 student took a rather indirect route to leadership in a student pharmaceutical …

High-Throughput Analysis Of Antimalarial Susceptibility Data By The Worldwide Antimalarial Resistance Network (Wwarn) In Vitro Analysis And Reporting Tool, Charles J. Woodrow, Sabina Dahlström, Richard Cooksey, Jennifer A. Flegg, Hervé Le Nagard, France Mentré, Claribel Murillo, Didier Ménard, François Nosten, Kanlaya Sriprawat, Lise Musset, Neils B. Quashie, Pharath Lim, Rick M. Fairhurst, Sam L. Nsobya, Veronique Sinou, Harald Noedl, Bruno Pradines, Jacob D. Johnson, Philippe J. Guerin, Carol H. Sibley, Jacques Le Bras

United States Army: Publications

Assessment of in vitro susceptibility is a fundamental component of antimalarial surveillance studies, but wide variations in the measurement of parasite growth and the calculation of inhibitory constants make comparisons of data from different laboratories difficult. Here we describe a Web-based, high-throughput in vitro analysis and reporting tool (IVART) generating inhibitory constants for large data sets. Fourteen primary data sets examining laboratory-determined susceptibility to artemisinin derivatives and artemisinin combination therapy partner drugs were collated from 11 laboratories. Drug concentrations associated with half-maximal inhibition of growth (IC₅₀s) were determined by a modified sigmoid E_max model-fitting algorithm, allowing standardized …

National And Regional Estimates Of Term And Preterm Babies Born Small For Gestational Age In 138 Low-Income And Middle-Income Countries In 2010, Anne C.C. Lee, Joanne D. Katz, Hannah Blencowe, Simon Cousens, Naoko Kozuki, James M. Tielsch, +30 Additional Authors

Global Health Faculty Publications

Background

National estimates for the numbers of babies born small for gestational age and the comorbidity with preterm birth are unavailable. We aimed to estimate the prevalence of term and preterm babies born small for gestational age (term-SGA and preterm-SGA), and the relation to low birthweight (<2500 >g), in 138 countries of low and middle income in 2010.

Methods

Small for gestational age was defined as lower than the 10th centile for fetal growth from the 1991 US national reference population. Data from 22 birth cohort studies (14 low-income and middle-income countries) and from the WHO Global Survey on Maternal …

Repairing The Antibiotic Pipeline: Can The Gain Act Do It?, Caitlin Forsyth

Washington Journal of Law, Technology & Arts

Antibiotic resistance, according to the World Health Organization, is one of the greatest threats to public health. To combat the problem, new antibiotics need to be developed. However, antibiotic research and development is fraught with scientific and economic problems. Recognizing these problems and the public health threat posed by antibiotic resistance, Congress passed the GAIN Act, which President Obama signed into law in June 2012. The GAIN Act (Act) incentivizes pharmaceutical companies to invest in antibiotic research and development. This Article will outline the incentives in the Act and suggest why the Act may not solve the growing antibiotic resistance …

Benchmarks 2012, July 13, The Rockefeller University

BenchMarks 2012

BenchMarks. Friday, July 13, 2012

The community newsletter of The Rockefeller University

BenchMarks is published monthly and is distributed on the campus of The Rockefeller University. It produced by the Office of Communications and Public Affairs

Acute Glomerulonephritis In A Child With Chlamydia Pneumoniae Infection: A Case Report., Giovanna Vitaliti, Raffaele Falsaperla, Leandra Giunta, Giuseppina Spataro, Venerando Rapisarda, Mario Velardita, Giuseppe Nunnari, Piero Pavone

Division of Infectious Diseases and Environmental Medicine Faculty Papers

Background. Infectious diseases seem to be an important and independent risk factor for renal failure, but the underlying mechanism of renal involvement during some kinds of infectious diseases is still unclear, even if the literature data report immunomediated and/or autoimmune mechanisms to explain the pathogenic relationship between the two diseases. In paediatric patients, Chlamydia pneumoniae is a rare cause of renal complications and it may manifest in several ways, mainly involving the respiratory system, even if also renal and glomerulalr complications, have been described. Case Diagnosis/Treatment. Herein we report a case of a 3-year-old child who developed an acute glomerulonephritis …

In-Water Hull Cleaning And Filtration System: In-Water Cleaning Trials, Department Of Fisheries

Fisheries Occasional Publications

No abstract provided.

Rethinking The Composition Of A Rational Antibiotic Arsenal For The 21st Century, Margaret A. Riley, Sandra M. Robinson, Christopher M. Roy, Robert L. Dorit

Biological Sciences: Faculty Publications

The importance of the human microbiome in health may be the single most valuable development in our conception of the microbial world since Pasteur's germ theory of the 1860s. Its implications for our understanding of health and pathogenesis are profound. Coupled with the revolution in diagnostics that we are now witnessing - a revolution that changes medicine from a science of symptoms to a science of causes - we cannot continue to develop antibiotics as we have for the past 80 years. Instead, we need to usher in a new conception of the role of antibiotics in treatment: away from …

Effect Of Mobile Phone Short Message Service On Adherence Rates To Antihypertensives At The Family Medicine Clinic, Barbara Karau

Master of Medicine in Internal Medicine

Background: Systemic arterial hypertension is the main modifiable risk factor for development of cardiovascular disease, which is the number one cause of death in high income countries and is increasingly becoming a cause of concern in Sub-Saharan Africa (1). Awareness, management and overall control of hypertension in Sub-Saharan Africa is poor (2,3). Poor control stems from various causes; one being patient non-adherence with medication, a factor amenable to modification. The use of mobile health for behavioural change from low resource settings is gaining widespread use in management of communicable diseases (4,6). Mobile phone has the potential to significantly influence patients …

Initial Epidemic Area Is Strongly Associated With The Yearly Extent Of Soybean Rust Spread In North America, Christopher C. Mundt, La Rae D. Wallace, Tom W. Allen, Clayton A. Hollier, Robert C. Kemerait, Edward J. Sikora

Faculty Publications

Hosts of soybean rust (Phakopsora pachyrhizi) are sensitive to low temperatures, limiting this obligate parasite in the United States to overwintering sites in a restricted area along the Gulf Coast. This temperature sensitivity of soybean rust hosts allowed us to study spatial spread of epidemic invasions over similar territory for seven sequential years, 2005-2011. The epidemic front expanded slowly from early April through July, with the majority of expansion occurring from August through November. There was a 7.4-fold range of final epidemic extent (0.4-3.0 million km2) from the year of smallest final disease extent (2011) to that of the largest …

In-Water Hull Cleaning System Cost & Cost Benefit Analysis, Department Of Fisheries

Fisheries Occasional Publications

No abstract provided.

Human Leukocyte Antigens And Cellular Immune Responses To Anthrax Vaccine Adsorbed, Inna G. Ovsyannikova, V. Shane Pankratz, Robert A. Vierkant, Nicholas M. Pajewski, Conrad P. Quinn, Richard A. Kaslow, Robert M. Jacobson, Gregory A. Poland

United States Public Health Resources

Interindividual variations in vaccine-induced immune responses are in part due to host genetic polymorphisms in the human leukocyte antigen (HLA) and other gene families. This study examined associations between HLA genotypes, haplotypes, and homozygosity and protective antigen (PA)-specific cellular immune responses in healthy subjects following immunization with Anthrax Vaccine Adsorbed (AVA). While limited associations were observed between individual HLA alleles or haplotypes and variable lymphocyte proliferative (LP) responses to AVA, analyses of homozygosity supported the hypothesis of a “heterozygote advantage.” Individuals who were homozygous for any HLA locus demonstrated significantly lower PA-specific LP than subjects who were heterozygous at all …

Heterogeneous Nuclear Ribonucleoprotein K Supports Vesicular Stomatitis Virus Replication By Regulating Cell Survival And Cellular Gene Expression, Phat X. Dinh, Anshuman Das, Rodrigo Franco, Asit K. Pattnaik

School of Veterinary and Biomedical Sciences: Faculty Publications

The heterogeneous nuclear ribonucleoprotein K (hnRNP K) is a member of the family of hnRNPs and was recently shown in a genome-wide small interfering RNA (siRNA) screen to support vesicular stomatitis virus (VSV) growth. To decipher the role of hnRNP K in VSV infection, we conducted studies which suggest that the protein is required for VSV spreading. Virus binding to cells, entry, and nucleocapsid uncoating steps were not adversely affected in the absence of hnRNP K, whereas viral genome transcription and replication were reduced slightly. These results indicate that hnRNP K is likely involved in virus assembly and/or release from …

Beyond The Storms: Strengthening Preparedness, Response, & Resilience In The 21st Century, Dane S. Egli

Journal of Strategic Security

Looking Beyond the Storms of major events and reactionary tendencies to prevent future disasters—and continuing to fix things—the author introduces a fresh assessment in the wake of Superstorm Sandy, the vexing challenge of domestic shootings, and a persistent nationwide drought. This paper offers a refreshing perspective on the need for transformational and innovative thinking on preparedness, response, and resilience, as well as disaster management. Against the backdrop of 9-11 terrorist attacks and natural disasters such as hurricanes Katrina, Irene, and Sandy, this paper, highlights that we—as homeland security planners and policymakers—must look beyond the immediate demands of grant proposals and …

Digest Of The Philadelphia College Of Osteopathic Medicine (Summer 2013), Philadelphia College Of Osteopathic Medicine

Digest

The Summer issue of the Digest of the Philadelphia College of Osteopathic Medicine includes the following articles:

• Message from the President
• PCOM Updates
• Commencement 2013
• Development News
• Back in the (Founders') Day: Faculty Pioneers, 1899 to World War I
• Teach Me So I May Remember—Alumni: On Faculty Mentors and Awakeners
• My Father’s Precious Legacy: A Reflection On the Influence of Nicholas S. Nicholas, DO, upon His Sons, his Family, Philadelphia College of Osteopathic Medicine and the Osteopathic Profession
• Love and Change: A Health Crisis Compels a Longtime Physician to Reevaluate his Approach to Teaching and Practicing Preventative Patient Care
• Philosophical …

An Introduction To California's Greenhouse Gas Emission Trading Program, Tseming Yang

Faculty Publications

Even though climate change will require a global solution, the efforts of individual nations and sub-national governmental units have become increasingly important for demonstrating leadership in creating effective regulatory programs and possible solutions. California’s Global Warming Solutions Act of 2006, also referred to as A.B. 32, and the associated greenhouse gas emission trading program are one set of such efforts. With A.B. 32, the state of California has created one of the most comprehensive and complex climate change programs in the world – a legally binding set of mandates for the state government to reduce greenhouse gas (GHG) emissions to …

Biom 250n.30: Microbiology For The Health Sciences, Jarrod Leroy Pollock

University of Montana Course Syllabi

No abstract provided.

Zein: Novel Natural Polymer For Nanoparticle- And Film-Mediated Gene Delivery, Jessica D. Taylor

Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research

Gene delivery, the introduction of DNA into cells, is applicable to gene therapy, DNA vaccination, functional genomics and diagnostics, tissue engineering, and drug-eluting medical devices. Particulates incorporating DNA are promising vehicles for gene delivery and overcome some of the barriers that hinder successful gene transfer, with the ability to protect DNA and provide for controlled, localized, and sustained release and transfection. Furthermore, innovative new gene delivery strategies that incorporate DNA particulates or complexes within films or coatings for devices and scaffolds could further provide for controlled and sustained transfection at the site of implant. Zein, a hydrophobic protein from corn, …

Subglacial Lake Vostok (Antarctica) Accretion Ice Contains A Diverse Set Of Sequences From Aquatic, Marine And Sediment-Inhabiting Bacteria And Eukarya, Scott O. Rogers, Yury M. Shtarkman, Zeynep A. Koçer, Robyn Edgar, Ram S. Veerapneni, Tom D'Elia, Paul F. Morris

Biological Sciences Faculty Publications

Lake Vostok, the 7th largest (by volume) and 4th deepest lake on Earth, is covered by more than 3,700 m of ice, making it the largest subglacial lake known. The combination of cold, heat (from possible hydrothermal activity), pressure (from the overriding glacier), limited nutrients and complete darkness presents extreme challenges to life. Here, we report metagenomic/metatranscriptomic sequence analyses from four accretion ice sections from the Vostok 5G ice core. Two sections accreted in the vicinity of an embayment on the southwestern end of the lake, and the other two represented part of the southern main basin. We obtained 3,507 …

The Bioarchaeology Of Changes In Social Stratification, Warfare, And Habitual Activities Among Iron Age Samnites Of Central Italy, Vitale Sparacello

Anthropology ETDs

This study uses a bioarchaeological approach to investigate the behavioral and social correlates of changes in skeletal properties during the Iron Age of central Italy. This was a period of demographic growth, increase in sociopolitical complexity, and social stratification. Early states were developing from simpler forms of social aggregation, and expansionistic, large-scale warfare was beginning. I analyzed the mechanical properties of Iron Age human skeletons (844 individuals) from 11 necropoleis belonging to the Oscan ethno-linguistic group (Samnites). The analysis of grave goods allowed for a division of individuals by status categories. Iron Age samples show an increase in stature compared …

The Immunological Mechanisms Of Influenza Vaccination: A Comparison Between A Seasonal Subunit Vaccine And An H5n1 Subunit Vaccine With And Without Alum Adjuvant, Sarah Elizabeth Vaughan

Biomedical Sciences ETDs

Highly pathogenic avian influenza (HPAI) H5N1 is an emerging infectious virus with a 60% fatality rate in humans. In the United States, a vaccine for H5N1 has been developed and stockpiled using FDA approved methods for seasonal vaccines; however, the H5N1 vaccine was shown to be less immunogenic than seasonal vaccines when evaluated in clinical trials. Adjuvants can be used to enhance the immune response to antigens. For the studies described herein, a lethal mouse model of H5N1 infection was utilized to examine the immune response to the H5N1 vaccine with and without the addition of an alum adjuvant, and …

Needle Stick Injuries--Risk And Preventive Factors: A Study Among Health Care Workers In Tertiary Care Hospitals In Pakistan., Asad Ali Khan Afridi, Ameet Kumar, Raza Sayani

Community Health Sciences

BACKGROUND:

Health care workers (HCWs) are at substantial risk of acquiring blood borne infections such as HIV, Hepatitis-B and Hepatitis-C through needle stick injuries (NSIs). This study aimed to assess the proportion of NSIs and their associated factors among HCWs and also to identify the areas in which preventive efforts might be directed to protect against this occupational hazard.

METHODOLOGY:

A cross-sectional study was conducted in two tertiary care hospitals of Pakistan representing both private and public health sector. A total of 497 HCWs (doctors and nurses) were interviewed using a structured questionnaire. Data was collected from January to May …