Digital Commons Network^™

Dysregulated Methylation At Imprinted Genes In Prostate Tumor Tissue Detected By Methylation Microarray., Daniel I Jacobs, Yingying Mao, Alan Fu, William Kevin Kelly, Yong Zhu

Department of Medical Oncology Faculty Papers

BACKGROUND: Imprinting is an important epigenetic regulator of gene expression that is often disrupted in cancer. While loss of imprinting (LOI) has been reported for two genes in prostate cancer (IGF2 and TFPI2), disease-related changes in methylation across all imprinted gene regions has not been investigated.

METHODS: Using an Illumina Infinium Methylation Assay, we analyzed methylation of 396 CpG sites in the promoter regions of 56 genes in a pooled sample of 12 pairs of prostate tumor and adjacent normal tissue. Selected LOI identified from the array was validated using the Sequenom EpiTYPER assay for individual samples and further confirmed …

Ezh2 Knockdown Suppresses The Growth And Invasion Of Human Inflammatory Breast Cancer Cells., Zhaomei Mu, Hua Li, Sandra V Fernandez, Katherine R Alpaugh, Rugang Zhang, Massimo Cristofanilli

Department of Medical Oncology Faculty Papers

INTRODUCTION: Inflammatory breast cancer (IBC) is the most metastatic variant of breast cancer with the poorest survival in all types of breast cancer patients and presently therapeutic targets for IBC are very limited. Enhancer of zeste homolog 2 (EZH2) is frequently expressed in human IBC and its expression positively correlates with worse clinical outcome. However, the molecular basis for EZH2 promoting IBC has not been explored. Here, we investigated the functional role of EZH2 in IBC cells by examining the effects of its knockdown on the formation of tumor spheroids and invasion of these cells in vitro and in vivo …

The Complex Transcriptional Landscape Of The Anucleate Human Platelet., Paul F. Bray, Steven E. Mckenzie, Leonard Edelstein, Srikanth Nagalla, Kathleen Delgrosso, Adam Ertel, Joan Kupper, Yi Jing, Eric R. Londin, Phillipe Loher, Huang-Wen Chen, Paolo Fortina, Isidore Rigoutsos

Cardeza Foundation for Hematologic Research

BACKGROUND: Human blood platelets are essential to maintaining normal hemostasis, and platelet dysfunction often causes bleeding or thrombosis. Estimates of genome-wide platelet RNA expression using microarrays have provided insights to the platelet transcriptome but were limited by the number of known transcripts. The goal of this effort was to deep-sequence RNA from leukocyte-depleted platelets to capture the complex profile of all expressed transcripts.

RESULTS: From each of four healthy individuals we generated long RNA (≥40 nucleotides) profiles from total and ribosomal-RNA depleted RNA preparations, as well as short RNA (<40 >nucleotides) profiles. Analysis of ~1 billion reads revealed that coding …

The Effectiveness Of An Abbreviated Training Program For Health Workers In Breast Cancer Awareness: Innovative Strategies For Resource Constrained Environments, Miriam Mutebi, Ronald Wasike, Ahmed Mushtaq, Aideed Kahie, Stephen Ntoburi

General Surgery, East Africa

Background: Breast cancer is characterized by late presentation and significant morbidity and mortality in developing countries. Breast screening aids in early detection of breast cancer. Nurses are uniquely placed to provide advocacy and screening in a resource limited environment.

Objectives: To assess the effectiveness of an abbreviated training program in breast cancer awareness on nurses at a tertiary hospital, in a resource constrained environment.

Methods: Using a statistical tool, the Solomon Model, 79 nurses were identified and divided into experimental and control groups. An abbreviated training intervention in breast cancer awareness was administered to the experimental group. Pre and post …

Exploring Public-Private Partnership In Preschool Education Provision: Potential For Socio-Economic Development, Vitaly Vitaly Radsky, Aynur Nabiyeva, Ulviyya Mikayilova

Khazar Journal of Humanities and Social Sciences

This paper explores the role of public-private partnerships in expanding access to preschool education. The first part of the paper summarizes global findings on the importance of quality early childhood education (ECE) for child development, participation of parents in the labor market and overall human capital development of a society. The second section provides a brief overview of the current ECE situation in Azerbaijan. The final section presents a community-based preschool model that was piloted in the Shamkir region by the Center for Innovations in Education (CIE), a Baku-based nonprofit organization as a model for expansion of ECE education in …

The Effect Of Birth Order On Age At First Sex And Total Number Of Lifetime Sexual Partners, Lacey Christine Clark Anderson

Theses and Dissertations

Research in the field of birth order is expansive and has been used to explain many social processes, including the sexual behavior of young adults. However, results regarding the influence of birth order on risky sexual behavior are somewhat limited, sometimes contradictory, and often lack generalizability to larger populations. In this thesis, using

data from the National Health and Social Life Survey (1992) and multiple regression techniques, I investigate the influence of ordinal position on two measures of sexual behavior,age at first sex and total number of lifetime partners, among young adults age 18 to 30. Results show that birth …

Historical Hydroclimatic Change At Theodore Roosevelt National Park: 1895 - 2011, Rhonda Jan Fietzek-Devries

Theses and Dissertations

Although numerous studies have examined hydroclimatic and climatic changes in our national parks, no previous studies have examined historical temperature, precipitation and hydroclimatic changes at Theodore Roosevelt National Park (South Unit). Documenting historical trends in temperature, precipitation and hydroclimatic variables is important for understanding present and future changes in vegetation and wildlife.

I used 117 (1895 - 2011) years of temperature and precipitation data obtained from the PRISM data network to construct the historical climatic water budget time series based upon the Thornthwaite water-budget model. Trend lines and descriptive statistics are used to analyze the monthly, seasonal and annual climatic …

Interprofessonal Education: Two Analyses Of Revised Instruments, Comparisons Of Professions, And Effectiveness Of An Interprofessional Health Care Course, Andrew Knight

Theses and Dissertations

The purposes of study #1 were to assess the reliability of the Readiness for Interprofessional Learning Scale (RIPLS-2010, Parsell & Bligh, 1999) instrument that was used in an Interprofessional Health Care (IPHC) course at University of North Dakota from 2010-2012 and compare students by discipline. The IPHC course curriculum team includes the director of Interprofessional Education, a course coordinator, and one faculty representative from medicine, nursing, physical therapy, occupational therapy, social work, music therapy, communication science and disorders, and nutrition/dietetics. The purposes of study #2 included an analysis of the reliability of a different version of the RIPLS (called RIPLS-2013) …

Effects Of Prazosin On Nightmares In Adults With Posttraumatic Stress Disorder, Laurie A. Tefft

Theses and Dissertations

The Diagnostic and Statistical Manual of Mental Disorders (American Psychiatric Association [APA], 2000) estimates that in the United States, Posttraumatic Stress Disorder (PTSD) affects 8% of adults at some point during their life. Nightmares have a severe impact and can cause great distress for individuals with PTSD (Ohayon & Shapiro, 2000). Spoormaker and Montgomery (2008) suggested that sleep problems may be more of a risk factor for PTSD then a symptom of the disorder. Either way, it is evident that sleep needs to be addressed in those with this diagnosis. Posttraumatic nightmares are seldom the topic of PTSD studies utilizing …

Identity On Trial: The Gabrielino Tongva Quest For Federal Recognition, Alice Mirlesse

Pomona Senior Theses

In this paper, the author looks at the impact of the policy of federal recognition on a Los Angeles basin Native community: the Gabrielino Tongva. The first section, the literature review focuses on the difficulties of defining “indigenousness” in the academic and political realms, as well as looking at Native scholars’ conceptualization of this unique and multifaceted identity. After a consideration of the theoretical framework of the study, the crossroads between anthropology and public policy analysis, the author presents the tools she used in her study, namely: participant observation, key-informant interviews, and the analysis of published documents and personal files. …

Spatial And Size Distribution Of Red Drum Caught And Released In Tampa Bay, Florida, And Factors Associated With Post-Release Hooking Mortality, Kerry E. Flaherty, Brent L. Winner, Julie L. Vecchio, Theodore S. Switzer

Gulf and Caribbean Research

The recreational fishery for red drum (Sciaenops ocellatus) in Florida is unusual in that most red drum targeted are immature and caught within estuarine waters. Current state regulations rely exclusively on bag and size limits, resulting in the release of a large proportion of captured individuals. This study employed hook-and-line sampling conducted monthly in Tampa Bay, Florida and catch-and-release mortality experiments to determine the spatial and size distribution of red drum and the mortality rate of released fish, respectively. Of the 1,405 red drum collected, more than 70% were smaller than the minimum legal size (457 mm standard …

Til Recession Do Us Part: Booms, Busts, And Divorce In The United States, Abdur Chowdhury

Economics Faculty Research and Publications

A general hypothesis regarding the impact of permanent income levels and business cycle fluctuations on divorce rate at the state level in the United States is analysed in this article. Based on the data for 45 states over the sample period of 1978–2009, it is shown that the higher the level of transitory income, the higher the incidence of divorce. In other words, divorce is pro-cyclical.

A Novel Resistance Exercise Machine For Use In A Lower Body Negative Pressure Box To Counteract The Effects Of Weightlessness, Christine M. Dailey

Doctoral Dissertations and Master's Theses

Physiological deconditioning is a critical problem in space, especially during long-term missions. Resistance exercise coupled with lower body negative pressure (LBNP) has been shown to be effective in counteracting some of the deconditioning related problems. This thesis describes the development of a compact and effective resistance exercise machine that works within an existing environmentally controlled LBNP Box and is designed to simulate both exercise and sitting, to prevent microgravity-induced deconditioning by simulating physiological and biomechanical features of upright exercise and daily activities. Theoretical calculations are carried out to determine whether kinematics, musculoskeletal loadings, and metabolic rate during supine exercise within …

Diagnosis Of Schistosoma Mansoni Without The Stool: Comparison Of Three Diagnostic Tests To Detect Schiostosoma Mansoni Infection From Filtered Urine In Zambia, Nilanjan Lodh, James C. L. Mwansa, Mabel M. Mutengo, Clive J. Shiff

Clinical Lab Sciences Faculty Research and Publications

Diagnosis for intestinal Schistosoma mansoni lacks sensitivity and is arduous to conduct. The standard diagnostic tests, Kato-Katz (KK) and circulating cathodic antigen (CCA) both lack sensitivity and with KK, require obtaining, transporting, and examining fresh stool. We compared diagnostic efficacy of KK, CCA, and polymerase chain reaction (PCR) to detect S. mansoni infection (species-specific DNA) from 89 filtered urine samples collected in Zambia. The PCR was the strongest indicator of positive cases with sensitivity and specificity of 100% in comparison to CCA (67% and 60%) and KK (50% and 100%). High positive and negative predictive values (100%) were also indicative …

The Impact Of Work-Limiting Disabilities On Earnings And Income Mobility, Nicholas A. Jolly

Economics Faculty Research and Publications

This article uses the 1968–2007 waves of the Panel Study of Income Dynamics (PSID) to examine how work-limiting disabilities influence the intragenerational earnings and income mobility of individuals. The results show that work-limiting disabilities increase the probability of downward mobility for several years after onset. Furthermore, the probability of being in the bottom portions of the distributions increases significantly, not only during the year of onset but also for at least 10 years afterwards. These results are more pronounced for those individuals suffering from a disability that is more chronic or severe in nature. Income from spousal earnings and government …

Intertemporal Valuation Of River Restoration, Andrew G. Meyer

Economics Faculty Research and Publications

Willingness to pay for an environmental improvement is a function of how long it takes to deliver the improvement. To measure the effect of time on benefits, I utilize a discrete choice experiment that includes an attribute for delay until the improvement occurs and simultaneously estimate discount rates and valuation parameters. I estimate the present value of immediate and delayed Minnesota River Basin improvements using discount rates directly estimated from the econometric model. Compared to an immediate river basin cleanup, Minnesota residents lose almost half of the benefits when cleanup is delayed by 5 years.

An Examination Of Personal Values And Value Systems Of Chinese And U.S. Business Students, Don E. Giacomino, Xin Li, Michael D. Akers

Accounting Faculty Research and Publications

Using the Rokeach Value Survey and the Musser and Orke typology this paper examines the personal values and value systems of business students in China and compares the results with the results of a recent study that used similar methodology to examine the values and value systems of U.S. students. The study also examines the differences in values and value systems of the Chinese students by gender and by major. While there are few differences for the Chinese students by gender, our findings show several differences in the rankings of values by the Chinese and U.S. students as well as …

Sex Offenders' Perceptions Of Mandated Group Treatment, Ashley Knipp

Theses and Dissertations

The objective of this study was to examine the perceptions of sex offenders about their mandated outpatient group treatment. The investigator developed an 81−item questionnaire that inquired about respondent characteristics and history; curriculum content; group therapy process; group facilitator characteristics; program policies and procedures; and offender perspectives on program strengths and weaknesses. The sample consisted of 31 male sex offenders who had all served prison sentences for a sexual offense and were involved in mandated outpatient treatment at 1 of 3 private, community−based clinics. The participants were diverse in regard to ethnicity, level of education, and marital status; they had …

May It Please The Environment?: A Study Of The Role Regional Location Plays In Influencing Federal Court Decisions On Fossil Fuel Cases, Stephen Perrott

Senior Independent Study Theses

No abstract provided.

Lavinia's Voice: Verbal And Nonverbal Expression In Shakespearean Performance, Kelly Kilgore

Electronic Theses and Dissertations

For my MFA internship requirement, I currently serve as an acting intern at the Orlando Shakespeare Theater in Partnership with UCF. I was cast as Lavinia in OST’s spring production of Titus Andronicus, and I will use this as my thesis role. It will be the very last show of my MFA career, and it will provide an exceptional opportunity for me to utilize all the skills learned during my three years of MFA classes and training. Jim Helsinger, Artistic Director at the Orlando Shakespeare Theater and Visiting Assistant Professor at the University of Central Florida, will direct the production. …

Boy Meets Boy: Envisioning Queer Youth Novels For Translation To The Stage, Daniel Davis

Electronic Theses and Dissertations

Queer Youth, or young people who identify as lesbian, gay, bisexual, transgender, or questioning, is a demographic with an increasing presence in contemporary society. Along with this increased presence has come an increase in support groups available to these youths that range from Gay-Straight Alliances at their schools, community groups from their local gay and lesbian center to, most recently, theater companies that have begun to offer workshops and performance opportunities within the Queer Youth Theatre genre. Queer Youth Theatre is an emerging form of topical theatre that deals with issues and situations queer youth may face in their daily …

9/11: We Will Forget, Jason Nettle

Electronic Theses and Dissertations

This study is based on the events of September 11, 2001. I will be writing a one man show containing fictional characters that I will write based on research of that day. The show will consist of numerous characters cut from different ages, religions, genders, and points of view. I will be portraying these characters using the tools I have acquired in studio and on stage. The challenge will be to make each character different and bring their experience and unique point of view to the performance. This study will begin at conception of the idea and follow the journey …

Interactionz: Engaging Lgbtq+ Youth Using Theatre For Social Change, Jonathan Jackson

Electronic Theses and Dissertations

Theatre for social change is a term used to describe a wide range of theatre-based techniques and methods. Through implementation of performance techniques, participants are encouraged to creatively explore and communicate various ideas with the specific intention of eliciting a societal or political shift within a given community. Through this thesis, I will explore the impact of applying theatre for social change in a youth-centered environment. I will discuss my journey as creator, facilitator, and project director of interACTionZ, a queer youth theatre program in Orlando, FL formed through a partnership between Theatre UCF at the University of Central Florida …

Historical Catch Trends And Efficacy Of Video Surveillance Monitoring Of Catch Per Unit Effort At Recreational Fishing Piers In South Carolina, Charles Christian Johnson

Electronic Theses and Dissertations

In recent years, recreational anglers claim declining catch rates in the shore-based pier fishery in South Carolina. The study seeks to evaluate whether catch data supports angler testimony in this region, to examine the relationship of landings at piers in context of the regional level, and to test a method which would enhance the ability of managers to monitor landings of this under-studied fishery. Novel, non-standardized catch series extending back to 1973 were acquired from fishing piers and examined in an attempt to produce significant trends in measures of catch. King mackerel catch (Scomberomorus cavalla) was tested for correlation to …

Age-Associated Changes In The Neuroinflammatory Response To Toll-Like Receptor 4 And 9 Stimulation, Leah Beth Christensen

Graduate Student Theses, Dissertations, & Professional Papers

During the perinatal time period, the mammalian brain is developing rapidly and is particularly sensitive to inflammation. Inflammation during this time period may be linked to later neurological illness in humans. Disease-associated alterations in learning and behavior can be modeled in rodents using perinatal immune stimulation with either infectious agents or Toll-like receptor (TLR) agonists. Although the gestational period is a particularly sensitive time for neurodevelopment, it is not known for how long after birth this sensitivity persists. In mice, susceptibility to neurological infection declines dramatically during the first weeks of life. Therefore, we sought to compare the neuroinflammatory responses …

The Rise And Fall Of Bread In America, Amanda Benson

Honors Theses - Providence Campus

Over the last century bread has gone through cycles of acceptance and popularity in the United States. The pressure exerted on the American bread market by manufacturers’ advertising campaigns and various dietary trends has caused it to go through periods of acceptance and rejection. Before the industrialization of bread making, consumers held few negative views on bread and perceived it primarily as a form of sustenance. After its industrialization, the battle between the manufacturers and the neighborhood bakeries over consumers began. With manufacturers, such as Wonder Bread, trying to maximize profits and dominate the market, corporate leaders aimed to discourage …

Institutional Vs. Non-Institutional Sources Of Presidential Influence: Explaining Congressional-Presidential Relations In The Age Of Polarization, Derek Culp

Electronic Theses and Dissertations

This thesis examines the determinants of presidential success with Congress. Seven essential sources of presidential power in the current era of party polarization were derived from the extant literature, and these factors were delineated into the institutional (formal) and non-institutional (informal) policymaking tools of the presidency. Variables that explain presidential legislative success include: intraparty support in Congress, the use of veto bargaining, executive orders and signing statements (institutional factors); as well as public approval, ‘going public,’ and strategic lobbying of Congress (non-institutional factors). Case studies of the presidencies of Bill Clinton and George W. Bush analyze the role of these …

Unintentionally Unethical: How Uncivil Leaders Violate Norms And Hurt Group Performance, Christopher Coultas

Electronic Theses and Dissertations

Incivility is a common form of low-grade aggression that lacks a clear intent to harm, that violates community norms and values for interpersonal conduct, and is often chronic in nature (Andersson & Pearson, 1999; Cortina, Magley, Williams, & Langhout, 2001). Because of its subtleties, it is difficult at times to detect and even more difficult to prevent. However, it is an essential phenomenon to research, due to its ubiquity and negative impact on worker outcomes such as job satisfaction and psychological health (Cortina et al., 2001). Incivility instigated by those in authority may be an even bigger problem, due to …

Personality Factors, Age, And Aggressive Driving: A Validation Using A Driving Simulator, Jose Vazquez

Electronic Theses and Dissertations

Two studies were conducted to investigate the relationship between age, personality factors and aggressive driving behavior. In Study 1, 1122 volunteers completed an online survey that included questionnaires on demographic data, personality factors, and driving behavior. Personality factors were measured using the Revised Competitiveness Index, the Sensation Seeking Scale, the Big Five Inventory, and the Cook Medley Hostility Scale, whereas aggressive driving behavior was measured using the Aggressive Driving Behavior Scale (ADBS). The majority of the volunteers were female (786 versus 336), while ages ranged from 18 to 87. In Study 2, 98 volunteers from Study 1 were recruited to …

The Working Alliance: A Survey Of An Outpatient Mental Health Center, Jennifer Bramstedt

All Graduate Theses, Dissertations, and Other Capstone Projects

This research investigated the working alliance between therapist and client at RiverView Clinic, an outpatient mental health center. Utilizing data captured by the Working Alliance Inventory-Short Revised (WAI-SR), the research posited the following:

• median total scores from therapist’s would be higher than median scores of the working alliance from clients
• clients who presented with mild to moderate concerns would have higher working alliance scores than clients who reported moderate to severe concerns
• new clients would have higher task and goal sub-scores than clients in therapy for more than fifteen sessions
• if working alliance scores reflected ruptures or strains in the …