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2013

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Effects Of Pasteurization (Heating) On Milled Flaxseed Quality, Cody Troy Turner Jan 2013

Effects Of Pasteurization (Heating) On Milled Flaxseed Quality, Cody Troy Turner

Cereal Science

Current food industry practices include the use of pasteurization (heat treatment) as a means to reduce the microbial counts of flaxseed (Linum usitatissimum). Flaxseed was pasteurized using a dry air heat treatment of 148 °C for 16.25 minutes and the oxidative stability was observed over a 20 week storage period. Flaxseed quality was measured using peroxide value, percent free fatty acid (as oleic), and propanal content. Peroxide values of the pasteurized milled flaxseed were found to significantly increase (P ≤ 0.05) by week 2. Propanal formation coincided with peroxide value development over 20 weeks of storage. Pasteurized flaxseed milled under …

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Characterization Of Edible Bean Flours: Properties And Functionality, Courtney W. Simons Jan 2013

Characterization Of Edible Bean Flours: Properties And Functionality, Courtney W. Simons

Cereal Science

Consumption of pulses is considered part of a healthy diet. Therefore, the opportunity exists for development of new pulse-based ingredients. However, a better understanding of their properties is necessary. The compositional and functional properties will vary depending on the bean type, their physical form (pre-cooked, raw flour, starch or protein fractionates) and growing location. In this study, edible bean flours (pinto, navy, black and small red) were subjected to extrusion cooking to produce snacks and texturized high-protein flour. The extrudates were studied to determine the effect of extrusion on the physical, physicochemical, chemical, sensory, and digestibility properties. Texturized high-protein flour …

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An Analysis Of The Educational Value And Impact Of A Dual, Versus Single, Robotic Surgical Console In The Training Of Obgyn Residents, Joseph E. Patruno Md, Hubert K. Huang Ms, Med, Michelle W. Huang Md, Thomas Hutchinson Md, Martin A. Martino Md Jan 2013

An Analysis Of The Educational Value And Impact Of A Dual, Versus Single, Robotic Surgical Console In The Training Of Obgyn Residents, Joseph E. Patruno Md, Hubert K. Huang Ms, Med, Michelle W. Huang Md, Thomas Hutchinson Md, Martin A. Martino Md

Department of Obstetrics & Gynecology

No abstract provided.

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Investigating The Role For Il-21 In Rabies Virus Vaccine-Induced Immunity., Corin L Dorfmeier, Evgeni P Tzvetkov, Anthony Gatt, James P Mcgettigan Jan 2013

Investigating The Role For Il-21 In Rabies Virus Vaccine-Induced Immunity., Corin L Dorfmeier, Evgeni P Tzvetkov, Anthony Gatt, James P Mcgettigan

Department of Microbiology and Immunology Faculty Papers

Over two-thirds of the world's population lives in regions where rabies is endemic, resulting in over 15 million people receiving multi-dose post-exposure prophylaxis (PEP) and over 55,000 deaths per year globally. A major goal in rabies virus (RABV) research is to develop a single-dose PEP that would simplify vaccination protocols, reduce costs associated with RABV prevention, and save lives. Protection against RABV infections requires virus neutralizing antibodies; however, factors influencing the development of protective RABV-specific B cell responses remain to be elucidated. Here we used a mouse model of IL-21 receptor-deficiency (IL-21R-/-) to characterize the role for IL-21 in RABV …

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Comparison Of Heterologous Prime-Boost Strategies Against Human Immunodeficiency Virus Type 1 Gag Using Negative Stranded Rna Viruses., Tessa M Lawrence, Celestine N Wanjalla, Emily A Gomme, Christoph Wirblich, Anthony Gatt, Elena Carnero, Adolfo García-Sastre, Douglas S Lyles, James P Mcgettigan, Matthias J Schnell Jan 2013

Comparison Of Heterologous Prime-Boost Strategies Against Human Immunodeficiency Virus Type 1 Gag Using Negative Stranded Rna Viruses., Tessa M Lawrence, Celestine N Wanjalla, Emily A Gomme, Christoph Wirblich, Anthony Gatt, Elena Carnero, Adolfo García-Sastre, Douglas S Lyles, James P Mcgettigan, Matthias J Schnell

Department of Microbiology and Immunology Faculty Papers

This study analyzed a heterologous prime-boost vaccine approach against HIV-1 using three different antigenically unrelated negative-stranded viruses (NSV) expressing HIV-1 Gag as vaccine vectors: rabies virus (RABV), vesicular stomatitis virus (VSV) and Newcastle disease virus (NDV). We hypothesized that this approach would result in more robust cellular immune responses than those achieved with the use of any of the vaccines alone in a homologous prime-boost regimen. To this end, we primed BALB/c mice with each of the NSV-based vectors. Primed mice were rested for thirty-five days after which we administered a second immunization with the same or heterologous NSV-Gag viruses. …

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Vegetative Filter Strip: A Best Management Practice (Bmp) For Feedlot Runoff Pollution Control In North Dakota, Md. Atikur Rahman Jan 2013

Vegetative Filter Strip: A Best Management Practice (Bmp) For Feedlot Runoff Pollution Control In North Dakota, Md. Atikur Rahman

Agribusiness and Biosystems Engineering

Runoff from animal feeding operations is a major source of water pollution. Vegetative filter strips (VFS) are effective ways to reduce nonpoint source pollution. In this study, vegetative filter strips with different designs and in climatic and management conditions of North Dakota were evaluated. Runoff samples were collected from inflow (before entering VFS) and outflow (after exiting the VFS) locations using automatic samplers. Collected samples were analyzed for solids and nutrients. It was observed that the transport reductions by VFS were ranged from very low to up to 100%. However, soluble nutrients were not as effectively removed as sediment and …

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Non-Destructive Analysis Of Single Plant Canola (Brassica Napus) Seed Using Near Infrared Spectroscopy, Harjot Sidhu Jan 2013

Non-Destructive Analysis Of Single Plant Canola (Brassica Napus) Seed Using Near Infrared Spectroscopy, Harjot Sidhu

Agribusiness and Biosystems Engineering

Near Infrared Spectroscopy (NIRS) is widely used for quantitative analysis of oilseeds in a non-destructive manner. Canola (Brassica napus) is a popular oilseed crop that is used for food and biofuel markets. Due to limited seed availability in plant breeding programs, single plant analysis is often preferred. An NIRS commercial calibration model was evaluated to predict single plant canola seed, but the results showed the need for new NIRS calibration models to predict moisture content, oil content, and fatty acid content for single plant canola seed (3 g) with minimal sample preparation. A separate NIRS calibration model was developed for …

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The Role Of Maternal Protein Intake During Late Gestation On Placental Vascular Function, Leslie Ann Lekatz Jan 2013

The Role Of Maternal Protein Intake During Late Gestation On Placental Vascular Function, Leslie Ann Lekatz

Animal Sciences

Global nutrient restriction or excess can influence umbilical hemodynamics in sheep fetuses (Chapter 2). We hypothesized that a specific component of the diet, namely maternal metabolizable protein (MP), would alter placental function. When MP restriction during late gestation occurs, we hypothesized that there would be a decrease in the sensitivity to bradykinin (BK) of the placental vascular arteries. In experiment 1, ewes received one of three isocaloric dietary treatments during late gestation: MP60: 60% of MP requirements; MP80: 80% of MP requirements; and MP100: 100% of the MP requirements on a dry matter basis from day 100 to 130 of …

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The Effect Of Artificial Insemination Or Natural Service Breeding Systems On Reproductive Performance, Calving Characteristics, Weaning Characteristics, And Steer Backgrounding Performance In A Commercial Beef Herd, Philip Lee Steichen Jan 2013

The Effect Of Artificial Insemination Or Natural Service Breeding Systems On Reproductive Performance, Calving Characteristics, Weaning Characteristics, And Steer Backgrounding Performance In A Commercial Beef Herd, Philip Lee Steichen

Animal Sciences

Two experiments were conducted to evaluate the effects of beef cattle breeding systems that incorporate artificial insemination (AI) or breeding systems that use natural service mating on resultant reproductive performance, calving characteristics, weaning characteristics, and steer backgrounding performance. In Exp. 1, cows and heifers were assigned to 1 of 2 breeding season systems: 1) exposed only to natural service bulls (NS, n = 541) or 2) bred via AI on the first day of the breeding season, followed by exposure to natural service bulls (TAI, n = 535). Final pregnancy rates were similar among treatments. However, more calves were born …

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Impact Of Environmental Factors On Ovarian Function And Endocrine Activity In Livestock, Samantha Kaminski Jan 2013

Impact Of Environmental Factors On Ovarian Function And Endocrine Activity In Livestock, Samantha Kaminski

Animal Sciences

Ovarian function is important in reproductive efficiency since the ovary is the site of oocyte development, as well as production and secretion of hormones necessary for proper follicle development, ovulation, and maintenance of pregnancy. However, many environmental factors can affect female reproductive function. For example, physical exercise during gestation has previously demonstrated to increase availability of nutrients and oxygen to the developing offspring, decrease mortality rates, and increase birth weights. Unfortunately there is limited information regarding ovarian development and fertility of offspring born to exercised mothers. Nutrition has also been demonstrated to affect reproductive function by altering steroid and metabolic …

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Improving Diabetes Merchandising, One Shelf At A Time, Caroline Christine Godwin Jan 2013

Improving Diabetes Merchandising, One Shelf At A Time, Caroline Christine Godwin

Apparel Design and Hospitality Management

The outcome of this study is to propose more-effective ways to merchandise diabetes products, with a cross-merchandised approach that provides education and a better in-store experience for shoppers. This research aims to help shoppers by proposing and testing—through in-store intercepts and a questionnaire—a well organized and comprehensive merchandising approach for drugstores, specifically Walgreens and Rite Aid. The results of the questionnaire indicate that the proposed merchandising solution (see Appendix B) provided welcomed improvements to the following areas: 1) organization of products and cross-merchandise product options, 2) shelf signage/navigation, and 3) education. Questionnaire respondents reported that the proposed merchandising solution offered …

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Isolated Laryngeal Leishmaniasis In Immunocompetent Patients: An Underdiagnosed Disease., Salvatore Cocuzza, Alessio Strazzulla, Marilia Rita Pinzone, Stefano Cosentino, Agostino Serra, Rosario Caltabiano, Salvatore Lanzafame, Bruno Cacopardo, Giuseppe Nunnari Jan 2013

Isolated Laryngeal Leishmaniasis In Immunocompetent Patients: An Underdiagnosed Disease., Salvatore Cocuzza, Alessio Strazzulla, Marilia Rita Pinzone, Stefano Cosentino, Agostino Serra, Rosario Caltabiano, Salvatore Lanzafame, Bruno Cacopardo, Giuseppe Nunnari

Department of Microbiology and Immunology Faculty Papers

We describe a case of isolated primary laryngeal leishmaniasis in an immunocompetent Italian patient with a previous medical history negative for visceral or cutaneous leishmaniasis, presenting with hoarseness. We also summarize the epidemiological, clinical, and diagnostic features and the therapeutic management of other cases of laryngeal leishmaniasis in immunocompetent subjects, described in the literature. Considering the insidious and nonspecific clinical presentation, the increasing number of different forms of mild or underestimated immunosuppressive conditions, and the number of people travelling in endemic zones, along with the ability of Leishmania amastigotes to survive for a long period in the body, we believe …

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Explaining Adolescent Behavior Intention To Consume Fast Food Using The Theory Of Planned Behavior, Julie Lynn Fudge Jan 2013

Explaining Adolescent Behavior Intention To Consume Fast Food Using The Theory Of Planned Behavior, Julie Lynn Fudge

Communication

This study tested the utility of the theory of planned behavior (TPB) to explain adolescent fast-food consumption among 349 high school adolescents. Subjective norms were further investigated to identify how parents and friends influenced adolescent fast-food consumption. Study participants completed a paper-based questionnaire measuring adolescent attitude, subjective norms for parents and friends, perceived behavioral control, and behavioral intention. Path analysis revealed that TPB explained adolescent fast-food behavioral intention to consume fast food. The model identified parent subjective norms had the strongest relationship with adolescent behavioral intention to consume fast food. Parent norms differed across age and grade in high school. …

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Two Coiled-Coil Domains Of Chlamydia Trachomatis Inca Affect Membrane Fusion Events During Infection., Erik Ronzone, Fabienne Paumet Jan 2013

Two Coiled-Coil Domains Of Chlamydia Trachomatis Inca Affect Membrane Fusion Events During Infection., Erik Ronzone, Fabienne Paumet

Department of Microbiology and Immunology Faculty Papers

Chlamydia trachomatis replicates in a parasitophorous membrane-bound compartment called an inclusion. The inclusions corrupt host vesicle trafficking networks to avoid the degradative endolysosomal pathway but promote fusion with each other in order to sustain higher bacterial loads in a process known as homotypic fusion. The Chlamydia protein IncA (Inclusion protein A) appears to play central roles in both these processes as it participates to homotypic fusion and inhibits endocytic SNARE-mediated membrane fusion. How IncA selectively inhibits or activates membrane fusion remains poorly understood. In this study, we analyzed the spatial and molecular determinants of IncA's fusogenic and inhibitory functions. Using …

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Tonight It’S Government Funded: A Rhetorical Analysis Of Manufactured Social Controversy And Government Funding Of The Arts, Kay Marie Beckermann Jan 2013

Tonight It’S Government Funded: A Rhetorical Analysis Of Manufactured Social Controversy And Government Funding Of The Arts, Kay Marie Beckermann

Communication

Thomas Goodnight’s definition of controversy offers an initial examination of Reverend Donald Wildmon and Reverend Pat Robertson’s attack of the National Endowment for the Arts (NEA), initiating the Culture Wars of 1989. Using their most reliable communication tactics, Wildmon and Robertson attempted to garner support for their values by manufacturing controversy related to government funding of the National Endowment for the Arts. Together, they manufacture social controversy around two inter-related themes, one of morality, in which they argued Christians were being persecuted by the art community, and the other against federal funding of objectionable art, using Andres Serrano and Robert …

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"Giving Rope And Pulling It Back" : Types And Patterns Of Strategies Used By Parents To Prevent Adolescent Substance (Ab)Use, Shweta Sharma Jan 2013

"Giving Rope And Pulling It Back" : Types And Patterns Of Strategies Used By Parents To Prevent Adolescent Substance (Ab)Use, Shweta Sharma

Communication

This study contributes to the INC theory by adding the stage of prevention to the parent-child relationships' timeline. Ten parents (six mothers and four fathers) were interviewed, and a memory enhancing timeline calendar was used to encourage parents to recall and talk about when they were trying to prevent substance (ab)use. Prevention strategies were coded on the basis of Inconsistent Nurturing as Control (INC) theory. Nurturing strategies consisted of 1.) Promoting open discussions, 2.) Monitoring behavior and activities in respectful ways, and 3.) Presenting a model for responsible use. Punishing strategies consisted of 1.) Reinforcing discipline and rules, 2.) Forcing …

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Incivility In The Informal Workplace: A Case Study Of Emerald Lutheran Church, Jared Nathan Fougner Jan 2013

Incivility In The Informal Workplace: A Case Study Of Emerald Lutheran Church, Jared Nathan Fougner

Communication

This study examined how incivility is expressed in an informal church organization and the impact relationships among those in the organization have on incivility. The researcher had staff members of the church complete journals. The researcher also observed the staff for one week and then conducted interviews based on the observations and journals. The researcher then coded data and grouped it into overarching themes. Themes that emerged about displays of incivility included: interrupting or not respecting another's ideas and yelling and gossiping behind each other's backs. The researcher examined these themes in terms of the relationships that exist among these …

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The Role Of Platelet-Derived Growth Factor Receptor Signaling In Medulloblastoma Metastasis, Kruttika Narayan Bhat Jan 2013

The Role Of Platelet-Derived Growth Factor Receptor Signaling In Medulloblastoma Metastasis, Kruttika Narayan Bhat

Cellular and Molecular Biology

Medulloblastoma is the most common brain tumor in children and one third of the patients remain incurable. Tumor metastasis is one of the primary reasons for its high mortality rate. Despite evidence of overexpression of PDGFRα and PDGFRβ in metastatic medulloblastoma, their individual roles remain controversial and equivocal. Analysis of their specific signaling pathway in medulloblastoma cells revealed that PDGFRα and PDGFRβ signaling events lead to distinct cellular functions: while PDGFRβ stimulated cell proliferation and invasion, the expression of CD44 to regulate progression via c-Myc and inhibited cell death, PDGFRα displayed the opposite effects. Studies also revealed that c-Myc plays …

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Effects Of Environmental Estrogens On The Growth Hormone-Insulin-Like Growth Factor System In Rainbow Trout (Oncorhynchus Mykiss), Andrea M. Hanson Jan 2013

Effects Of Environmental Estrogens On The Growth Hormone-Insulin-Like Growth Factor System In Rainbow Trout (Oncorhynchus Mykiss), Andrea M. Hanson

Cellular and Molecular Biology

The increasing production, use, and disposal of an expanding array of chemicals that enter the environment pose a serious threat to terrestrial and aquatic animals, as well as to humans. Fish in aquatic habitats are exposed to increasing concentrations of environmental contaminants, including environmental estrogens (EE). In this work, rainbow trout were used to assess the effects of EE on the growth hormone (GH)-insulin-like growth factor (IGF) system, specifically focusing on osmoregulation, organismal growth, and growth at the molecular level. Juvenile trout were exposed to varying concentrations of 17â-estradiol (E2), â-sitosterol (âS), and 4-n-nonylphenol (NP) in vivo and in vitro. …

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Identifying Causes Of Dissolved Oxygen Depletion And Determination Of Sediment Oxygen Demand In The Souris River, Matthew Ernest Baker Jan 2013

Identifying Causes Of Dissolved Oxygen Depletion And Determination Of Sediment Oxygen Demand In The Souris River, Matthew Ernest Baker

Civil and Environmental Engineering

The Upper Souris River was placed on the Environmental Protection Agencies (EPA) impaired waters list for low dissolved oxygen (DO). A Total Maximum Daily Load (TMDL) study was conducted to determine possible causes of DO depletion. From sampling and site visits it was determined nonpoint sources contributed the majority of organic loadings to the Upper Souris River. Through preliminary testing, it was determined that sediment oxygen demand (SOD) played a key role in depleting DO levels during winter months and required further investigation. River profile surveying, water quality sampling, and laboratory testing of SOD were carried out to determine parameters …

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Skeletal Cell Differentiation Is Enhanced By Atmospheric Dielectric Barrier Discharge Plasma Treatment., Marla J Steinbeck, Natalie Chernets, Jun Zhang, Deepa S Kurpad, Gregory Fridman, Alexander Fridman, Theresa A. Freeman Jan 2013

Skeletal Cell Differentiation Is Enhanced By Atmospheric Dielectric Barrier Discharge Plasma Treatment., Marla J Steinbeck, Natalie Chernets, Jun Zhang, Deepa S Kurpad, Gregory Fridman, Alexander Fridman, Theresa A. Freeman

Department of Orthopaedic Surgery Faculty Papers

Enhancing chondrogenic and osteogenic differentiation is of paramount importance in providing effective regenerative therapies and improving the rate of fracture healing. This study investigated the potential of non-thermal atmospheric dielectric barrier discharge plasma (NT-plasma) to enhance chondrocyte and osteoblast proliferation and differentiation. Although the exact mechanism by which NT-plasma interacts with cells is undefined, it is known that during treatment the atmosphere is ionized generating extracellular reactive oxygen and nitrogen species (ROS and RNS) and an electric field. Appropriate NT-plasma conditions were determined using lactate-dehydrogenase release, flow cytometric live/dead assay, flow cytometric cell cycle analysis, and Western blots to evaluate …

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An Investigation Into Bromate Formation In Ozone Disinfection Systems, Leslee Storlie Jan 2013

An Investigation Into Bromate Formation In Ozone Disinfection Systems, Leslee Storlie

Civil and Environmental Engineering

Ozonation is used as an alternative disinfection process to chlorination but unfortunately has a potential of oxidizing bromide, a natural component of water sources, to bromate. Bromate is a possible carcinogen with a maximum contaminant level of 10 ppb. To understand bromate formation in full-scale systems, a comprehensive study was conducted at the Moorhead Water Treatment Plant (WTP). Bromide concentrations in source waters were monitored. Water samples from locations in the ozonation chambers were collected and analyzed for bromate and other parameters. Results showed that bromate formation was increased through increases in pH, bromide, and ozone dose during high temperatures …

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Investigation Of The International Food-Grade Soybean Market, Changmin Kim Jan 2013

Investigation Of The International Food-Grade Soybean Market, Changmin Kim

Agribusiness and Applied Economics

This study provides an overview of the international soybean market and takes a look at a case study of an exporter and their target markets, China, Japan, and South Korea. Then it estimates future soybean import demands for these countries. Results indicate that China’s soybean import demand will remarkably increase compared with those in Japan and South Korea. Although Korean and Japanese buyers are willing to pay a premium for food-grade soybeans, China market may grow faster than those in Japan and South Korea. This is because as income increases, Chinese consumer may concern more about their health which makes …

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Determinants Of Capital Structure In Agricultural Cooperatives In North Dakota, Ran Shang Jan 2013

Determinants Of Capital Structure In Agricultural Cooperatives In North Dakota, Ran Shang

Agribusiness and Applied Economics

This thesis analyzes how the optimal capital structure is affected by capital management and major sources of risk under the rule of maximizing the value of discounted cash flows to members. The analysis is done by using the present value of cash flow method. This research employs the panel procedure in Statistical Analysis System (SAS) to solve the firm value optimization problem. The data set includes financial reports from farm supply and grain marketing cooperatives in North Dakota. Empirical Results indicate that the optimal debt ratio is related to the lagged debt ratio, the proportion of assets held as liquid …

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An Assessment Of Integrated Weed Management Strategies For Purple Threeawn-Dominated Rangelands, Nickolas Alois Dufek Jan 2013

An Assessment Of Integrated Weed Management Strategies For Purple Threeawn-Dominated Rangelands, Nickolas Alois Dufek

Range Science

Purple threeawn (Aristida purpurea Nutt.) is a native bunch grass that is avoided by grazers. It is capable of dominating old cropland and overgrazed pastures, limiting livestock carrying capacity, and degrading wildlife habitat. Traditional management tools have had little impact on threeawn dominance in semiarid regions of the west. Our objectives were to: 1) assess fire and nitrogen treatment effects on threeawn forage quality at various phenological stages to test their potential as pretreatments in a grazing strategy and 2) examine a threeawn-dominated plant community’s response to prescribed fire, nitrogen addition, and clipping. Fire improved threeawn forage quality with greater …

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Possible Steps Of Complete Disassembly Of Post-Termination Complex By Yeast Eef3 Deduced From Inhibition By Translocation Inhibitors., Shinya Kurata, Ben Shen, Jun O Liu, Nono Takeuchi, Akira Kaji, Hideko Kaji Jan 2013

Possible Steps Of Complete Disassembly Of Post-Termination Complex By Yeast Eef3 Deduced From Inhibition By Translocation Inhibitors., Shinya Kurata, Ben Shen, Jun O Liu, Nono Takeuchi, Akira Kaji, Hideko Kaji

Department of Biochemistry and Molecular Biology Faculty Papers

Ribosomes, after one round of translation, must be recycled so that the next round of translation can occur. Complete disassembly of post-termination ribosomal complex (PoTC) in yeast for the recycling consists of three reactions: release of tRNA, release of mRNA and splitting of ribosomes, catalyzed by eukaryotic elongation factor 3 (eEF3) and ATP. Here, we show that translocation inhibitors cycloheximide and lactimidomycin inhibited all three reactions. Cycloheximide is a non-competitive inhibitor of both eEF3 and ATP. The inhibition was observed regardless of the way PoTC was prepared with either release factors or puromycin. Paromomycin not only inhibited all three reactions …

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Dynein And Dynactin Leverage Their Bivalent Character To Form A High-Affinity Interaction., Amanda E Siglin, Shangjin Sun, Jeffrey K Moore, Sarah Tan, Martin Poenie, James D Lear, Tatyana Polenova, John A Cooper, John C Williams Jan 2013

Dynein And Dynactin Leverage Their Bivalent Character To Form A High-Affinity Interaction., Amanda E Siglin, Shangjin Sun, Jeffrey K Moore, Sarah Tan, Martin Poenie, James D Lear, Tatyana Polenova, John A Cooper, John C Williams

Department of Biochemistry and Molecular Biology Faculty Papers

Cytoplasmic dynein and dynactin participate in retrograde transport of organelles, checkpoint signaling and cell division. The principal subunits that mediate this interaction are the dynein intermediate chain (IC) and the dynactin p150(Glued); however, the interface and mechanism that regulates this interaction remains poorly defined. Herein, we use multiple methods to show the N-terminus of mammalian dynein IC, residues 10-44, is sufficient for binding p150(Glued). Consistent with this mapping, monoclonal antibodies that antagonize the dynein-dynactin interaction also bind to this region of the IC. Furthermore, double and triple alanine point mutations spanning residues 6 to 19 in the yeast IC homolog, …

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Ash2 Acts As An Ecdysone Receptor Coactivator By Stabilizing The Histone Methyltransferase Trr., Albert Carbonell, Alexander Mazo, Florenci Serras, Montserrat Corominas Jan 2013

Ash2 Acts As An Ecdysone Receptor Coactivator By Stabilizing The Histone Methyltransferase Trr., Albert Carbonell, Alexander Mazo, Florenci Serras, Montserrat Corominas

Department of Biochemistry and Molecular Biology Faculty Papers

The molting hormone ecdysone triggers chromatin changes via histone modifications that are important for gene regulation. On hormone activation, the ecdysone receptor (EcR) binds to the SET domain-containing histone H3 methyltransferase trithorax-related protein (Trr). Methylation of histone H3 at lysine 4 (H3K4me), which is associated with transcriptional activation, requires several cofactors, including Ash2. We find that ash2 mutants have severe defects in pupariation and metamorphosis due to a lack of activation of ecdysone-responsive genes. This transcriptional defect is caused by the absence of the H3K4me3 marks set by Trr in these genes. We present evidence that Ash2 interacts with Trr …

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Molecular Determinants Of Epidermal Growth Factor Binding: A Molecular Dynamics Study., Jeffrey M Sanders, Matthew E Wampole, Mathew L. Thakur, Eric Wickstrom Jan 2013

Molecular Determinants Of Epidermal Growth Factor Binding: A Molecular Dynamics Study., Jeffrey M Sanders, Matthew E Wampole, Mathew L. Thakur, Eric Wickstrom

Department of Biochemistry and Molecular Biology Faculty Papers

The epidermal growth factor receptor (EGFR) is a member of the receptor tyrosine kinase family that plays a role in multiple cellular processes. Activation of EGFR requires binding of a ligand on the extracellular domain to promote conformational changes leading to dimerization and transphosphorylation of intracellular kinase domains. Seven ligands are known to bind EGFR with affinities ranging from sub-nanomolar to near micromolar dissociation constants. In the case of EGFR, distinct conformational states assumed upon binding a ligand is thought to be a determining factor in activation of a downstream signaling network. Previous biochemical studies suggest the existence of both …

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The Tip Of The Tail Needle Affects The Rate Of Dna Delivery By Bacteriophage P22., Justin C Leavitt, Lasha Gogokhia, Eddie B Gilcrease, Anshul Bhardwaj, Gino Cingolani, Sherwood R Casjens Jan 2013

The Tip Of The Tail Needle Affects The Rate Of Dna Delivery By Bacteriophage P22., Justin C Leavitt, Lasha Gogokhia, Eddie B Gilcrease, Anshul Bhardwaj, Gino Cingolani, Sherwood R Casjens

Department of Biochemistry and Molecular Biology Faculty Papers

The P22-like bacteriophages have short tails. Their virions bind to their polysaccharide receptors through six trimeric tailspike proteins that surround the tail tip. These short tails also have a trimeric needle protein that extends beyond the tailspikes from the center of the tail tip, in a position that suggests that it should make first contact with the host's outer membrane during the infection process. The base of the needle serves as a plug that keeps the DNA in the virion, but role of the needle during adsorption and DNA injection is not well understood. Among the P22-like phages are needle …

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