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Articles 16021 - 16050 of 19585
Full-Text Articles in Entire DC Network
The Efficacy Of Crude Aqueous Extracts Of Some Plants As Grain Protectants Against The Stored Grain Mite, Rhizoglyphus Tritici, Muhammad Bashir, Muhammad Dildar Gogi, Muhammad Ashfaq, Dr. Muhammad Afzal, Muhammad Ahsan Khan, Muhammad Ihsan
The Efficacy Of Crude Aqueous Extracts Of Some Plants As Grain Protectants Against The Stored Grain Mite, Rhizoglyphus Tritici, Muhammad Bashir, Muhammad Dildar Gogi, Muhammad Ashfaq, Dr. Muhammad Afzal, Muhammad Ahsan Khan, Muhammad Ihsan
Turkish Journal of Agriculture and Forestry
Mites are one of the most notorious and destructive pests affecting stored grains, contaminating stored grain with toxins and reducing germination rates. Applying synthetic miticides is the sole method for providing knockdown control of mites; however, the continuous application of miticides results in the contamination of grain with highly persistent toxic residues, which pose a health hazard to humans. The present research evaluates the efficacy of crude aqueous extracts of Azadirachta indica, Melia azedarach, Eucalyptus sp., Datura stramonium, and Citrullus colocynthis under laboratory conditions at 5 concentrations (6.25%, 12.5%, 25%, 50%, and 100%) and 4 exposure periods (7, 14, 21, …
Possibilities For Reducing Tractor Engine Friction Losses At Cold Start Using An Ultrasonic Irradiation Technique, Florin Mariasiu
Possibilities For Reducing Tractor Engine Friction Losses At Cold Start Using An Ultrasonic Irradiation Technique, Florin Mariasiu
Turkish Journal of Agriculture and Forestry
Challenges to the effectiveness of specific activities and production technologies in agriculture become more pressing each day as worldwide demand for agricultural products increases. The mechanization of agriculture brings positive quantitative and qualitative effects; however, the modernization of production tools (tractors and agricultural machinery) needs to be directly linked to increasing economic efficiency of use. Period of use and life expectancy of agricultural tractors can be increased by reducing the friction and wear that occurs among moving mechanical parts. A special case in point is the cold start process in compression ignition engines. In a cold start great forces develop, …
Insecticidal Activity Of Essential Oil Of Prangos Ferulacea (Umbelliferae) Against Ephestia Kuehniella (Lepidoptera: Pyralidae) And Trichogramma Embryophagum (Hymenoptera: Trichogrammatidae), Fahri̇ye Ercan, Hati̇ce Baş, Murat Koç, Di̇lek Pandir, Sevcan Öztemi̇z
Insecticidal Activity Of Essential Oil Of Prangos Ferulacea (Umbelliferae) Against Ephestia Kuehniella (Lepidoptera: Pyralidae) And Trichogramma Embryophagum (Hymenoptera: Trichogrammatidae), Fahri̇ye Ercan, Hati̇ce Baş, Murat Koç, Di̇lek Pandir, Sevcan Öztemi̇z
Turkish Journal of Agriculture and Forestry
Essential oil vapors obtained by the hydrodistillation of Prangos ferulacea (Umbelliferae) were tested on the different stages of Ephestia kuehniella Zeller (Lepidoptera: Pyralidae) and egg parasitoid Trichogramma embryophagum Hartig (Hymenoptera: Trichogrammatidae). Extracts of the volatile fractions from P. ferulacea were analyzed by capillary gas chromatography-mass spectrometry. The major compound of the essential oil was detected as 2,3,6-trimethyl benzaldehyde (66.59%) and the minor compound was heneicosane (0.02%). The third instar larvae of E. kuehniella (LC_{50}: 379.662 µL L^{-1} air and LC_{99}: 538.755 µL L^{-1} air) and the pupal stage of T. embryophagum (LC_{50}: 5.947 µL L^{-1} air and LC_{99}: 19.568 µL …
Mathematical Modelling At Secondary School: The Macsi-Clongowes Wood College Experience, J.P.F. Charpin, S. O'Hara, Dana Mackey
Mathematical Modelling At Secondary School: The Macsi-Clongowes Wood College Experience, J.P.F. Charpin, S. O'Hara, Dana Mackey
Articles
In Ireland, to encourage the study of STEM (science, technology, engineering and mathematics) subjects and particularly mathematics, the Mathematics Applications Consortium for Science and Industry (MACSI) and Clongowes Wood College (County Kildare, Ireland) organized a mathematical modelling workshop for senior cycle secondary school students. Participants developed simple mathematical models for everyday life problems with an open-ended answer. The format and content of the workshop are described and feedback from both students and participating teachers is provided. For nearly all participants, this workshop was an enjoyable experience which showed mathematics and other STEM components in a very positive way.
Utilizing Social Media To Build Community In The Residence Halls, Michael Jeremy Courson
Utilizing Social Media To Build Community In The Residence Halls, Michael Jeremy Courson
LSU Master's Theses
The purposes of this study are to understand how community is built and residents are educated in the residence halls on college campuses as well as to design a method for social media to aid that process. To achieve this, documents were collected from 10 different institutions from across the country. The documents were analyzed to determine how community is built, residents are educated, and the use of social media in residence life. The results showed that none of the institutions were using social media in any form. It also showed that all of the institutions build community and educate …
The Use Of Duddingtonia Flagrans For Gastrointestinal Parasitic Nematode Control In Feces Of Exotic Artiodactylids At Disney's Animal Kingdom®, Jenna Terry
LSU Master's Theses
Gastrointestinal nematodes (GIN) are parasites of major concern for domestic and exotic ruminant species around the world. In the past, zoological facilities used anthelmintics as their primary control method. Challenges in accurate dosing and administration of anthelmintics to exotic hoofstock contributed to the development of resistant nematode populations in zoological settings. The historic dependency on anthelmintics to control GIN populations is no longer an option. Biological alternatives are urgently needed, in both exotic and domestic ruminants, in the war against resistance. One such alternative is the use of the nematophagous fungus, Duddingtonia flagrans. Three independent studies were conducted: A nine …
The Search For Catalytic Subunit Of Dna Polymerase Α In Thermomyces Lanuginosus, Chelsea Elyse Meier
The Search For Catalytic Subunit Of Dna Polymerase Α In Thermomyces Lanuginosus, Chelsea Elyse Meier
Honors Program Theses
In eukaryotes, the enzyme DNA polymerase a (DNAPa) begins the process of DNA replication. Ultimately, this means DNAPa is responsible for all cell proliferation and growth of an organism. The mechanism by which this enzyme operates is interesting due to its implications in medicine; however, that mechanism remains largely elusive. DNAPa, including its catalytic subunit, has been studied in several eukaryotes. Unfortunately, these organisms present issues with the ease of study and with ethics. This research delved into a novel model organism that was potentially devoid of all these issues: the fungus Thermomyces lanuginosus. Though T. lanuginosus was easy to …
Atp-Induced Changes In F-Actin Expression Via P2x7, Taylor Hircock
Atp-Induced Changes In F-Actin Expression Via P2x7, Taylor Hircock
Honors Program Theses
The purpose of this research project was to study the effects that extracellular ATP has on F-actin levels in cells containing the P2X7 purinergic receptor. MAC-T cells which express the receptor were exposed to various levels of ATP and stained to undergo flow cytometry. In this case, F-actin was labeled with 488 phalloidin which was able to be detected by the flow cytometer. This was done in conjuction with controls to show how ATP exposure affected Factin levels. Based on these results, the cytoskeletal changes taking place in the cell in response to ATP were able to be measured and …
Competition And Extrinsic Motivation In The Band Classroom: A Review Of Literature And Suggestions For Educational Practice, Stephanie Kay Opsal
Competition And Extrinsic Motivation In The Band Classroom: A Review Of Literature And Suggestions For Educational Practice, Stephanie Kay Opsal
Honors Program Theses
Public school bands teach students valuable artistic expression as they work together to learn and perform music in instrumental ensembles. Music educators who claim this purpose need to sustain student interest and involvement in band to continue the art form, because band is an elective course in which students join voluntarily. Factors that motivate students to participate in band include the intrinsic desire for music and the extrinsic stimuli for participation, including a large emphasis on competitions.
South Atlantic Mpas And Deepwater Coral Hapcs: Characterization Of Benthic Habitat And Fauna., Stacey Harter, Andrew David, John K. Reed
South Atlantic Mpas And Deepwater Coral Hapcs: Characterization Of Benthic Habitat And Fauna., Stacey Harter, Andrew David, John K. Reed
Faculty Scholarship
This cruise was conducted from July 6 to 19, 2012 in the previous reporting period, and a 731 page preliminary cruise report was submitted to the SAFMC on August 8, 2012 (Harter, Reed, David, and Farrington, 2012).
101 Books For Youth That Feature Hunters & Hunting, Leann Seddon
101 Books For Youth That Feature Hunters & Hunting, Leann Seddon
Graduate Research Papers
Librarians, teachers and other adults concerned with motivating reading among youth hunters want to provide them with books that feature the sport; however many sources cite a concern at the lack of positive, or at least neutral, portrayal of hunting in books for youth. There was also a lack of any comprehensive bibliography that identified books that feature hunters and hunting, yet alone one that identified how they were portrayed in those books. The purpose of this project was to create an annotated bibliography which would fill that gap and support recommendations for reading and purchase. The annotated bibliography consists …
If Rocks Could Talk: Using Central Place Foraging Theory In Lithic Production And Utilization Research, Sara Hocking
If Rocks Could Talk: Using Central Place Foraging Theory In Lithic Production And Utilization Research, Sara Hocking
Graduate Student Theses, Dissertations, & Professional Papers
This research addresses microeconomic decision making during the Fur-Trade Occupation at Housepit 54 of the Bridge River Site. Specifically, it addresses the lithic production in regard to field processing and technological investment at the site. Field processing considers the importance of cost in transporting lithic raw material, while technological investment examines the amount of retouch and usewear based upon the distance from the quarry. The research tests the hypothesis that: the farther away the quarry is from the village site, the more flakes will be dominated by late stage reduction while the extent of retouch and usewear per tool will …
Attitudes And Perceptions Of Community Councils On The Collaborative Forest Landscape Restoration Program And The Southwestern Crown Collaborative Forest Restoration Projects, Jimmie Mckay
Graduate Student Theses, Dissertations, & Professional Papers
This paper is designed to give the reader basic in-sight to the different attitudes and perception that local residents have in regards to the Collaborative Forest Landscape Restoration Program and the Southwestern Collaborative's ability to accomplish their mission.
Building Stories: Modernity, Socialization And Failure In Works Of Franz Kafka And Hannah Arendt, Chris Carpenter
Building Stories: Modernity, Socialization And Failure In Works Of Franz Kafka And Hannah Arendt, Chris Carpenter
University Honors Theses
For both Hannah Arendt and Franz Kafka modernity is a matter of loss. Arendt articulates this loss as coeval with the rise of 'society' and the subsumption of the public sphere by the private. Kafka is more oblique, but I believe his fiction describes the same kind of socialization process, a process that leads to alienation, loss of agency, and ultimately, failure. By reading Kafka through an Arendtian lens I show how each writer's conception of modernity complements the others. By integrating Walter Benjamin's influential reading, I show how Kafka's ideas of 'progress' and 'knowledge', subsumed under the social, inevitably …
Sex-Specific Effects Of Exercise Ancestry On Metabolic, Morphological And Gene Expression Phenotypes In Multiple Generations Of Mouse Offspring, Lisa M. Guth, Andrew T. Ludlow, Sarah Witkowski, Mallory R. Marshall, Laila C.J. Lima, Andrew C. Venezia, Tao Xiao, Mei Ling Ting Lee, Espen E. Spangenburg, Stephen M. Roth
Sex-Specific Effects Of Exercise Ancestry On Metabolic, Morphological And Gene Expression Phenotypes In Multiple Generations Of Mouse Offspring, Lisa M. Guth, Andrew T. Ludlow, Sarah Witkowski, Mallory R. Marshall, Laila C.J. Lima, Andrew C. Venezia, Tao Xiao, Mei Ling Ting Lee, Espen E. Spangenburg, Stephen M. Roth
Exercise and Sport Studies: Faculty Publications
New findings: • What is the central question of this study? How does parental exercise training directly and/or transgenerationally affect offspring phenotypes over two generations of mice? • What is the main finding and what is its importance? We observed preliminary evidence that parental exercise training can influence health-related phenotypes (e.g. body and fat mass, muscle gene expression) in mouse offspring. These findings indicate that parental exercise may be another environmental factor (like altered nutrition) capable of influencing offspring phenotypes in adulthood. Early life and preconception environmental stimuli can affect adult health-related phenotypes. Exercise training is an environmental stimulus affecting …
Evaluation Of The Live Release Rate Formula & Implementation: Example Analysis Of The Effects Of Mandatory Spay/Neuter Laws On Intake At Shelters Within Dallas, Tx, Amanda Laskoe
MPA/MPP/MPFM Capstone Projects
It is estimated that shelters spend $1 billion annually dealing with unwanted animals (Frank & Carlisle-Frank, 2007). Approximately six to eight million cats and dogs enter U.S. shelters each year, and three to four million of these animals are then euthanized (HSUS, 2009). These are staggering statistics, yet in the early nineties, euthanasia estimates ranged from seven to seventeen million dogs/cats annually (Frank & Carlisle-Frank, 2011). Such improvements have resulted from a greater understanding of, and resources being dedicated to, the issue. Unfortunately, the rate of decline for euthanasia has slowed because programs have not been accurately targeted toward animal …
Unrelated Business Income's Impact On Direct Public Support In The Nonprofit Sector, Kelsey Lewis
Unrelated Business Income's Impact On Direct Public Support In The Nonprofit Sector, Kelsey Lewis
MPA/MPP/MPFM Capstone Projects
Nonprofits today are losing their traditional funding sources for a variety of reasons and are turning to nontraditional ways of finding resources to make sure their organization is able to sustain itself while still meeting its mission. One common non-traditional approach is engaging in unrelated business income activities. Since these activities are not related to a nonprofit’s mission they are subject to taxation. Some question whether these activities help a nonprofit organization meet its mission.
A nonprofit seen not fulfilling its mission can be detrimental to that organization especially where its donors are concerned. If a donor does not support …
Evolutionary Biology For The 21st Century, Jonathan B. Losos, Stevan J. Arnold, Gill Bejerano, E. D. Brodie, David Hibbett, Hopi E. Hoekstra, David P. Mindell, Antónia Monteiro, Craig Moritz, H. Allen Orr, Dmitri A. Petrov, Susanne S. Renner, Robert E. Ricklefs, Pamela S. Soltis, Thomas L. Turner
Evolutionary Biology For The 21st Century, Jonathan B. Losos, Stevan J. Arnold, Gill Bejerano, E. D. Brodie, David Hibbett, Hopi E. Hoekstra, David P. Mindell, Antónia Monteiro, Craig Moritz, H. Allen Orr, Dmitri A. Petrov, Susanne S. Renner, Robert E. Ricklefs, Pamela S. Soltis, Thomas L. Turner
Biology
Theodosius Dobzhansky famously proclaimed that “nothing makes sense in biology except in the light of evolution." Though Dobzhansky's statement is sometimes dismissed by biologists in other fields as self-promotion, recent advances in many areas of biology have shown it to be prophetic. For example, genomics, which emerged mostly from molecular biology, is now steeped in evolutionary biology. Evolutionary theory helps to explain our origins, our history, and how we function as organisms and interact with other life forms, all of which are crucial to understanding our future (e.g., [1]–[5]). Evolutionary approaches have helped reconstruct the history of …
Thermal Selection In Sceloporus Occidentalis During Exercise Recovery, Morgan A. Halley
Thermal Selection In Sceloporus Occidentalis During Exercise Recovery, Morgan A. Halley
Scripps Senior Theses
Ectotherms regulate body temperature (Tb) primarily through behavioral interactions with their environment. These animals also have limited aerobic scopes and must rely on anaerobic metabolism to support intense activity; lactate byproduct and glycogen depletion are two consequences of anaerobic metabolism that must be dealt with during exercise recovery. It has been suggested that, in many ectothermic species, Tb may affect the rate of lactate clearance and glycogen repletion during recovery from intense exercise. This study investigated thermoregulatory behavior in Western Fence Lizards (Sceloporus occidentalis) to determine preferred Tb during exercise recovery. I hypothesized that …
Do Bha And Bht Induce Morphological Changes And Dna Double-Strand Breaks In Schizosaccharomyces Pombe?, Amy V. Tran
Do Bha And Bht Induce Morphological Changes And Dna Double-Strand Breaks In Schizosaccharomyces Pombe?, Amy V. Tran
Scripps Senior Theses
Butylated Hydroxyanisole, BHA, and Butylated Hydroxytoluene, BHT, are commonly used as preservatives for our food as well as additives in many products such as cosmetics, petroleum, and medicine. Although their use has been approved by the Food and Drug Administration (FDA), there have been controversies and debates on whether these phenol derivatives or antioxidants are safe to use. Their accumulative toxicology and side effects need to be thoroughly investigated as we continue to consume them on a daily basis. Data obtained by genomic analysis in Tang lab suggested the involvement of DNA damage checkpoint/repair pathways in the response network to …
Through The Bottom Of A Drinking Glass: How Beer And Brewing Changed Human History, Matthew Bougher
Through The Bottom Of A Drinking Glass: How Beer And Brewing Changed Human History, Matthew Bougher
Master of Liberal Studies Theses
Abstract Every corner of the earth is either currently influenced by or has been influenced by the beer and brewing industry. The significance of this product throughout human history is extensive and one would think the study of it would reflect this. However, until recently the study of beer and brewing was mostly historical and archeological in nature. The social, personal lives and economic impact of those who either consume or produce beer were largely overlooked. This look into how beer and brewing changed cultures and the life of everyone from the king to the peasant is of great importance …
Insects In The World Of Fiction, Erin Bauer
Insects In The World Of Fiction, Erin Bauer
Department of Entomology: Faculty Publications
Since early childhood, I have had a passion for two things: books and insects. While working on master’s degrees in library science and entomology, I’ve had the unique opportunity to combine these fields. My library background led to an interest in bibliographical information, and thus, as part of my master’s program in entomology at the University of Nebraska–Lincoln, I chose to research and compile a list of fictional works (published/produced in the United States, 1950–2012) about or including insects, spiders, related arthropods, and/or entomologists as main characters.
Although there are numerous small bibliographies about insects, a full list seems to …
Aquatic Macroinvertebrate Communities And Water Quality At Buffered And Non-Buffered Wetland Sites On Federal Waterfowl Production Areas In The Rainwater Basin, Nebraska, John R. Riens, Matt S. Schwarz, Fatima Mustafa, William Wyatt Hoback
Aquatic Macroinvertebrate Communities And Water Quality At Buffered And Non-Buffered Wetland Sites On Federal Waterfowl Production Areas In The Rainwater Basin, Nebraska, John R. Riens, Matt S. Schwarz, Fatima Mustafa, William Wyatt Hoback
Department of Entomology: Faculty Publications
Nebraska’s Rainwater Basin has an abundance of natural wetlands and is a focal point in the annual migration corridor used by millions of waterfowl and shorebirds. However, these wetlands are in a landscape dominated by agriculture and as a result, siltation and poor water quality are continual problems. We evaluated twelve wetland sites on federally managed Waterfowl Protection Areas from 2007 – 2009 for water quality, sediment quality, andmacroinvertebrate diversity. Six of the sites received agricultural runoff directly via culverts and drainage ditches (non-buffered sites) and six siteswere protected from agricultural runoff by a vegetated buffer (buffered sites). Mean total …
Towards The Elements Of Successful Insect Rnai, Jeffrey G. Scott, Kristin Michel, L. Bartholomay, Blair Siegfried, Wayne B. Hunter, Guy Smagghe, Kun Yan Zhu, Angela E. Douglas
Towards The Elements Of Successful Insect Rnai, Jeffrey G. Scott, Kristin Michel, L. Bartholomay, Blair Siegfried, Wayne B. Hunter, Guy Smagghe, Kun Yan Zhu, Angela E. Douglas
Department of Entomology: Faculty Publications
RNA interference (RNAi), the sequence-specific suppression of gene expression, offers great opportunities for insect science, especially to analyze gene function, manage pest populations, and reduce disease pathogens. The accumulating body of literature on insect RNAi has revealed that the efficiency of RNAi varies between different species, the mode of RNAi delivery, and the genes being targeted. There is also variation in the duration of transcript suppression. At present, we have a limited capacity to predict the ideal experimental strategy for RNAi of a particular gene/insect because of our incomplete understanding of whether and how the RNAi signal is amplified and …
Motivation In Athletes With And Without Autism Spectrum Disorder: Sq, Eq And Aq Relationships To Preferred Feedback, Julia C. Harreschou
Motivation In Athletes With And Without Autism Spectrum Disorder: Sq, Eq And Aq Relationships To Preferred Feedback, Julia C. Harreschou
Scripps Senior Theses
All athletes are driven by motivation, sources or reasons to push their bodies to their limits and continue to do so regularly. There have been several studies concerning motivation in typical athletes, and many regarding social motivation in people with High Functioning Autism (HFA), however most have been limited to children, and there have been no investigations into HFA athletes’ motivation. The current study looks into the role of social dimensions in athletics, and tests how one’s gender and placement on the Empathy Questionnaire (EQ), Systemizing Questionnaire (SQ), and on the Autism Questionnaire (AQ) affect intrinsic motivation in athletics. It …
Creating The Role Of Nicia In The Mandrake, Robert T. Krueger
Creating The Role Of Nicia In The Mandrake, Robert T. Krueger
All Graduate Theses, Dissertations, and Other Capstone Projects
This document is a thesis submitted in partial fulfillment of the Master of Fine Arts degree in theatre. It is a detailed account of author Robert Krueger's artistic process in creating the role of Nicia in Minnesota State University, Mankato's production of The Mandrake in the fall of 2012. The thesis chronicles the actor's artistic process from pre-production through performance in five chapters: a pre-production analysis, a historical and critical perspective, a rehearsal and performance journal, a post-production analysis and a process development analysis. Appendices and works cited are included.
Patterns Of Spanish-English Code-Switching In Children's Literature In The Us: The Use Of Español In Books Para Niños, Megan Rae Vasatka
Patterns Of Spanish-English Code-Switching In Children's Literature In The Us: The Use Of Español In Books Para Niños, Megan Rae Vasatka
All Graduate Theses, Dissertations, and Other Capstone Projects
Spanish-English code-switching is a prevalent and significant form of communication in bilingual and bicultural communities. Authors who wish to reflect and validate cultural and linguistic diversity in their written works may incorporate code-switching in their texts. The purpose of this study is to explore the growing trend of the inclusion of the Spanish language in English-based books written for children in the United States. In order to better understand how code-switching is utilized by authors of varying Spanish language proficiency, fourteen non-native Spanish speakers were surveyed and seventeen examples of their children's books that include Spanish-English code-switching were analyzed in …
Aboriginal Title In Canada: Site-Specific Or Territorial?, Kent Mcneil
Aboriginal Title In Canada: Site-Specific Or Territorial?, Kent Mcneil
All Papers
This paper addresses the issue of Aboriginal title to land, and the relationship I see between Indigenous law and the common law in this context. In my understanding, there have been three judicial approaches to Aboriginal title: 1. A purely proprietary approach, based on occupation of land and the effect given to occupation by the common law (common law Aboriginal title). 2. An Indigenous law approach, whereby Aboriginal title arises from and is defined by pre-existing Indigenous law (Indigenous law title). 3. A territorial approach, whereby Aboriginal title is derived from both common law and Indigenous law and has governmental …
What Determines The Market Success Of A Nanofood Innovation?, Emie Yiannaka
What Determines The Market Success Of A Nanofood Innovation?, Emie Yiannaka
Cornhusker Economics
If you are not quite sure what a nanofood is, you are not alone. The majority of the public in the United States is still uninformed about nanotechnology and its applications to the food sector. According to a commonly used definition, a nanofood is food that has been cultivated, produced, processed or packaged using nanotechnology techniques/tools or to which engineered nanomaterials have been added (Sekhon 2010).1 Food nanotechnology has the potential to increase the supply, quality and safety of food. Applications include the use of nanosensors for monitoring crop growth, pest control and plant and animal diseases; additives and ingredients …
Epa Spcc Rule, J. David Aiken
Epa Spcc Rule, J. David Aiken
Cornhusker Economics
Nebraska farmers and ranchers are concerned regarding meeting the May 10, 2013 deadline for preparing (but not submitting) Oil Spill Prevention, Control and Countermeasure (SPCC) plans. This newsletter discusses the SPCC program requirements for ag producers, explains the SPCC self-certification option and discusses SPCC implementation realities. Importantly, the Environmental Protection Agency (EPA) cannot legally enforce SPCC requirements against farms until September 30, 2013. EPA enforcement is likely to occur only if a “significant” (my word) oil products spill has occurred (a single spill of more than 1,000 gallons or two spills, each totaling more than 42 gallons within 12 months). …