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Microrna-34a Modulates Mdm4 Expression Via A Target Site In The Open Reading Frame, Pooja Mandke, Nicholas Wyatt, Jillian Fraser, Benjamin Bates, Steven J. Berberich, Michael P. Markey

Educator Preparation & Leadership Faculty Works

Background

MDM4, also called MDMX or HDMX in humans, is an important negative regulator of the p53 tumor suppressor. MDM4 is overexpressed in about 17% of all cancers and more frequently in some types, such as colon cancer or retinoblastoma. MDM4 is known to be post-translationally regulated by MDM2-mediated ubiquitination to decrease its protein levels in response to genotoxic stress, resulting in accumulation and activation of p53. At the transcriptional level, MDM4 gene regulation has been less clearly understood. We have reported that DNA damage triggers loss of MDM4 mRNA and a concurrent increase in p53 activity. These experiments attempt …

Pine Springs Hazardous Fuel Reduction Phase Ii, United States Department Of The Interior, Bureau Of Land Management

Environmental Assessments (UT)

The purpose of the Pine Springs Hazardous Fuel Reduction Phase II project is to reduce the buildup of hazardous fuels that have accumulated over the last several decades in order to prevent the potential for large catastrophic fire events. In addition, the proposed action is needed to maintain important sage-steppe habitat for a variety of wildlife species in the project area.

Development Of The Posttraumatic Stress Symptoms -- Childhood Obesity Model, Kristi Wilsman

Masters Theses & Specialist Projects

This project developed a model to account for an obesity outcome in children who have posttraumatic stress disorder (PTSD) and whose parents have posttraumatic stress symptoms (PTSS) or PTSD. A literature review provided the basis for the model and covered the areas of childhood obesity, parental PTSS, childhood PTSD, adverse childhood experiences, relational PTSD, ineffective parent support, and the stress response. A model to explain the outcome of obesity in children with PTSD as mediated by parental support provided after a traumatic event was developed: The Parental PTSSChildhood Obesity Model. The literature review supports a relational perspective for viewing child …

Endothelin-1 Induced Phosphorylation Of Erk1/2 In Bovine Corneal Endothelial Cells, Akhila Bethi

Masters Theses & Specialist Projects

The purpose of this study was to determine whether Endothelin-1 (ET-1) induced cellular responses in bovine corneal endothelial cells (BCECs) involves MAPK pathway by phosphorylating ERK1/2 protein kinase and to find out the phosphorylation patterns of ERK1/2 in confluent and sub-confluent cells. BCECs were isolated from bovine corneas and cultured in medium supplemented with 10% serum. Confluent (contact inhibited) and sub-confluent (actively growing cells) serum starved cells grown in T-75 flasks were treated with 10nM Endothelin-1. The control cells were left untreated. Total cellular protein was isolated using RIPA buffer and quantified according to the Peterson modification of the Lowry …

Plant-Like Cryptochrome Does Not Promote Blue Light-Induced Resetting Of The Circadian Clock In Chlamydomonas Reinhardtii, Jonathan Howton

Masters Theses & Specialist Projects

The circadian clock is an endogenous timer that allows an organism to anticipate and properly prepare for the daily changes in the environment. This preparation occurs in the form of daily rhythms in metabolism, physiology, and behavior. These approximately 24-hour rhythms are reset upon environmental time cues such as the daily light/dark and temperature cycles. The unicellular green alga Chlamydomonas reinhardtii is a useful model organism for circadian clock research. It shows several well-characterized circadian rhythms of behavior, and the monitoring of its rhythm of phototaxis, or swimming towards light, has been automated. The receptors involved in entraining the clock …

An Examination Of White-Nose Syndrome Occurrence And Dispersal Patterns: Utilizing Global And Local Moran's I Analysis To Evaluate An Emerging Pathogen, Celia M. Davis

Masters Theses & Specialist Projects

In this research, a novel approach that utilizes Moran’s I statistical analyses to examine the spatio-temporal dispersal patterns of the White-Nose Syndrome currently affecting North American bat species is undertaken to further understand the disease transmission mechanism(s) of this emerging wildlife epidemic. White-Nose Syndrome has been responsible for in excess of five million bat deaths to date and has the potential to alter the ecological landscape significantly; however, due to a variety of factors, little research has been conducted into the patterns of infection on a national scale. Global and Local Moran’s I analyses were performed on the spatial-temporal variable …

Effect Of Lion Calls On African Elephants (Loxodonta Africana) In Addo Elephant National Park, South Africa, Margaret Rose Hook

Masters Theses & Specialist Projects

Perceived predation risk alters animals’ behavior. This shift in behavior often comes at the cost of attaining resources. Generally, African elephants (Loxodonta africana) experience little predation pressure; however, the risk of predation by lions (Panthera leo) increases other prey species are less abundant. In elephant herds, related females and their offspring travel together in family groups, led by the eldest female. Response to predation pressure was examined by playing lion calls to the population of 437 elephants at the Main Camp Section of Addo Elephant National Park (AENP) in South Africa. Unfamiliar lion calls from a …

Next Generation Sequencing Reveals Gene Expression Patterns In The Zebrafish Inner Ear Following Growth Hormone Injection, Gopinath Rajadinakaran

Masters Theses & Specialist Projects

Loss of hair cells due to acoustic trauma results in the loss of hearing. In humans, unlike other vertebrates, the mechanism of hair cell regeneration is not possible. The molecular mechanisms that underlie this regeneration in nonmammalian vertebrates remain elusive. To understand the gene regulation during hair cell regeneration, our previous microarray study on zebrafish inner ears found that growth hormone (GH) was significantly upregulated after noise exposure. In this current study, we utilized Next Generation Sequencing (NGS) to examine the genes and pathways that are significantly regulated in the zebrafish inner ear following sound exposure and GH injection. Four …

Technosexuality: Technology, Sexuality, And Convergence, John M. Wolf

Mass Communications - Dissertations

Convergence refers to a phenomenon where multiple communication technologies are integrated into a single device. Technosexuality proposes a convergence between sexuality as a social phenomenon and technologically mediated modes of interpersonal communication and sexual information consumption. The findings of this study indicate that though there is not a complete convergence between technology and sexuality, there nonetheless exists a relationship between the two constructs. Consistent with extant literature about computer-mediated sexuality, the technosexual behaviors in this study were organized primarily by arousal type; however, subsequent degrees of classification suggest that technology also plays a decisive role in the ways in which …

Microbial Degradation Of 2,4-Dichlorophenoxyacetic Acid On The Greenland Ice Sheet, Marek Stibal, Jacob Baelum, William E. Holben, Sebastian R. Sørensen, Anders Jensen, Carsten S. Jacobsen

Biological Sciences Faculty Publications

The Greenland ice sheet (GrIS) receives organic carbon (OC) of anthropogenic origin, including pesticides, from the atmosphere and/or local sources, and the fate of these compounds in the ice is currently unknown. The ability of supraglacial heterotrophic microbes to mineralize different types of OC is likely a significant factor determining the fate of anthropogenic OC on the ice sheet. Here we determine the potential of the microbial community from the surface of the GrIS to mineralize the widely used herbicide 2,4-dichlorophenoxyacetic acid (2,4-D). Surface ice cores were collected and incubated for up to 529 days in microcosms simulating in situ …

Reversal By Rarα Agonist Am580 Of C-Myc-Induced Imbalance In Rarα/Rarγ Expression During Mmtv-Myc Tumorigenesis, Almudena Bosch, Silvina P. Bertran, Yongke Lu, Avalon Garcia, Alexis M. Jones, Marcia I. Dawson, Eduardo F. Farias

Pharmacology, Physiology and Toxicology

Introduction

Retinoic acid signaling plays key roles in embryonic development and in maintaining the differentiated status of adult tissues. Recently, the nuclear retinoic acid receptor (RAR) isotypes α, β and γ were found to play specific functions in the expansion and differentiation of the stem compartments of various tissues. For instance, RARγ appears to be involved in stem cell compartment expansion, while RARα and RARβ are implicated in the subsequent cell differentiation. We found that over-expressing c-Myc in normal mouse mammary epithelium and in a c-Myc-driven transgenic model of mammary cancer, disrupts the balance between RARγ and RARα/β in …

A Revision Of The Genus Mecistostethus Marseul (Histeridae, Histerinae, Exosternini), Michael S. Caterino, Alexey K. Tishechkin, Nicolas Degallier

Publications

We revise the genus Mecistostethus Marseul, sinking the monotypic genus Tarsilister Bruch as a junior synonym. Mecistostethus contains six valid species: M. pilifer Marseul, M. loretoensis (Bruch), comb. n., M. seagorum sp. n., M. carltoni sp. n., M. marseuli sp. n., and M. flechtmanni sp. n. The few existing records show the genus to be widespread in tropical and subtropical South America, from northern Argentina to western Amazonian Ecuador and French Guiana. Only a single host record associates one species with the ant
Pachycondyla striata Smith (Formicidae: Ponerinae), but it is possible that related ants host all the species.

A Genome-Wide Over-Expression Screen Identifies Genes Involved In Phagocytosis In The Human Protozoan Parasite, Entamoeba Histolytica, Ada V. King, Brenda H. Welter, Amrita B. Koushik, Lindsay N. Gordon, Lesly A. Temesvari

Publications

Functional genomics and forward genetics seek to assign function to all known genes in a genome. Entamoeba histolytica is a protozoan parasite for which forward genetics approaches have not been extensively applied. It is the causative agent of amoebic dysentery and liver abscess, and infection is prevalent in developing countries that cannot prevent its fecal-oral spread. It is responsible for considerable global morbidity and mortality. Given that the E. histolytica genome has been sequenced, it should be possible to apply genomic approaches to discover gene function. We used a genome-wide over-expression screen to uncover genes regulating an important virulence function …

Tremblings, August 2012, Western Aspen Alliance

Tremblings

No simple answer to "What killed the Aspen?"

Mary Lou Fairweather

The recent impacts to aspen in central Arizona are so apparent that I even get questioned at social gatherings: “Tell me again, what is killing the aspen?” The answer is complicated, and harkens back to a muddled media interview. I met the reporter at the Arboretum at Flagstaff and rambled on about drought, spring frost, fire suppression, forest succession, secondary insects and diseases, and ungulate browsing. “But what killed the aspen?” the reporter asked. Nearby an Arboretum docent led a group of visitors to a small aspen grove and …

Gonadotropin-Releasing Hormone Plasticity: A Comparative Perspective., T J Stevenson, T P Hahn, S A Macdougall-Shackleton, G F Ball

Brain and Mind Institute Researchers' Publications

Gonadotropin-releasing hormone 1 (GnRH1) is a key regulator of the reproductive neuroendocrine system in vertebrates. Recent developments have suggested that GnRH1 neurons exhibit far greater plasticity at the cellular and molecular levels than previously thought. Furthermore, there is growing evidence that sub-populations of GnRH1 neurons in the preoptic area are highly responsive to specific environmental and hormonal conditions. In this paper we discuss findings that reveal large variation in GnRH1 mRNA and protein expression that are regulated by social cues, photoperiod, and hormonal feedback. We draw upon studies using histochemistry and immediate early genes (e.g., c-FOS/ZENK) to illustrate that specific …

Scholarworks Statistics - August 2012, Scholarworks

ScholarWorks Reports

For the month of August 2012, ScholarWorks recorded a total of 17,305 full-text downloads and 14,822 page views.

Role Of The Leguminous Shrub Amorpha Canescens (Leadplant) In The Nebraska Sandhills Grasslands: Water Relations And Patterns Of Water Uptake, Adam Yarina

School of Natural Resources: Dissertations, Theses, and Student Research

This study investigated the ecophysiological role of Amorpha canescens, a leguminous shrub native to the Nebraska Sandhills. Although A. canescens is an important ecological player in the Sandhills, its impacts on the surrounding plant community are poorly understood. To remedy this, two sites were selected for study at the Gudmundsen Sandhills Laboratory near Whitman, Nebraska – one with A. canescens (G-L) and one without A. canescens (G-NL). Both sites contained five representative herbaceous species: two C₃ grasses (Hesperostipa comata and Koeleria macrantha), two C₄ grasses (Andropogon hallii and Calamovilfa longifolia), and one forb …

The Breathing Of The Bays, Jamie Vaudrey, Margaret (Peg) A. Van_Patten (Editor)

Wrack Lines

Bays in Long Island Sound and beyond have natural rhythms like breathing. Stress can impact the health of these natural ecosystems. While Long Island Sound has been examined for the extent of hypoxia in the summer, small embayments have not been examined carefully. In this article, two researchers investigate the oxygen, or lack of, in small bays of Long Island Sound.

Faculty Guidebook, August 2012, Lindenwood University

Employee Handbooks

Information and guidelines for Lindenwood University faculty.

Graduate Bulletin | 2012-2013, Jacksonville State University

Bulletins & Catalogs | Graduate

Vol. 34, No. 1 (August 2012). Jacksonville State was granted University status in 1966 and began adding graduate programs to its curriculum at that time. Graduate bulletins and catalogs contain information on the academic calendar, policies and procedures, faculty, scholarships and tuition, and degree requirements for graduate programs.

Effects Of Meloidogyne Incognita, Soil Physical Parameters, And Thielaviopsis Basicola On Cotton Root Architecture And Plant Growth, Jianbing Ma

Graduate Theses and Dissertations

The root-knot nematode, Meloidogyne incognita, and the seedling pathogen, Thielaviopsis basicola, commonly co-exist in Arkansas cotton fields and may interact resulting in increased losses. The primary objective of this research was to evaluate the effects of soil physical parameters on these soilborne pathogens and cotton growth in controlled environmental, field, and microplot studies. Controlled environmental experiments used two soil bulk densities and four pathogen treatments: non-infested soil, soil infested with M. incognita or T. basicola and soil infested with both pathogens. The results indicated bulk density generally did not affect seedling growth or disease since soils had low penetration resistance …

Genetic Diversity, Pathogenicity/Virulence Variation And Population Structure Of Rhizoctonia Spp. Associated With Rice Sheath Blight In Arkansas And Qtl Mapping For Disease Resistance, Vanina Lilian Castroagudin

Graduate Theses and Dissertations

Rice sheath blight (ShB) is one of the most prevalent diseases in rice. The significance of this disease is increasing due to more intensive production practices globally. Rhizoctonia solani Kühn AG 1 - IA [teleomorph: Thanatephorus cucumeris (A.B. Frank) Donk] is considered the primary ShB pathogen of rice. However, Rhizoctonia oryzae-sativae, Rhizoctonia oryzae, and R. solani AG 2 and AG 11, have also been isolated from sheath blight-like lesions on rice.Up to now, there is little information on genetic diversity and patterns of molecular evolution of the fungus, and there is no study on the structure of the R. solani …

Effects Of Environment And Genotype On Charcoal Rot Development On Soybeans, Micah Diane Doubledee

Graduate Theses and Dissertations

Charcoal rot of soybean, caused by the soilborne fungus Macrophomina phaseolina (Tassi) Goid, is a disease associated with high soil temperature and low soil moisture. Above-ground symptoms, which can be difficult to distinguish from drought symptoms, include low vigor, dead leaves that remain attached to the plant, early senescence and yield loss. Irrigation limits damage, but does not prevent colonization of the tissue by the pathogen. No soybean line is immune to M. phaseolina, but a few lines may have moderate resistance. The objectives of this research were to 1) determine the effects of genotype and drought on the development …

Ant Tribe, Yan Zhao

Graduate Theses and Dissertations

"Ant Tribe" describes the post-80s generation university graduates who live together in poor conditions without Social security in communities around China's major metropolises. They dream of a better life in big cities but struggle with low-paying jobs. These struggling "elites" have become the fourth weak Social group, after peasants, migrant workers and unemployed people. The reason why these college graduates are compared to ants is that they are like ants: clever, hardworking, politically weak and living in groups.

The real world is always different from the ideal world of the "Ant Tribe" in China. They often lose their purposes in …

Effects Of Testing Time On Anaerobic Toxicity Assessment, Omolola Atinuke Akintomide

Graduate Theses and Dissertations

Anaerobic digestion has been widely used for wastewater treatment for decades. Several Anaerobic Toxicity tests have been developed to screen wastewater for toxicity and assess the treatability of such toxicants. However, past research and reviews on Biochemical Methane Potential (BMP) test and Anaerobic Toxicity Assay (ATA) were considered in this study.

The experimental tests included ammonium-nitrogen and sodium chloride toxicity tests with varied concentration and solid retention time. These tests were designed to demonstrate the impact of ammonia- nitrogen and sodium chloride on the biological degradation process. The anaerobic test set-up includes bench-scale test reactors and respirometers used for measuring …

Fabrication And Characterization Of Biocomposites From Polylactic Acid And Bamboo Fibers, Sarah E. Royse

Department of Engineering Mechanics: Dissertations, Theses, and Student Research

Environmental concerns have been motivating research in the field of biodegradable materials, especially those from biological sources. Polylactic acid (PLA) is one biopolymer with the potential to replace some standard synthetic polymers. PLA is currently used for fibers, medical sutures, and some packaging, but is still used very little compared with synthetic polymers. One thing that can be done to expand the potential applications of PLA is to add fibers to create composite materials. Bamboo is a good choice for these fibers because it is abundant in many parts of the world, grows very quickly, and is widely unused. Composites …

2012 Summary Report On Institutional Effectiveness, Clemson University

Institutional Effectiveness Reports

No abstract provided.

Kulturkampf In Lomé: German And Ewe Identification And Alienation In Togoland, West Africa, 1884-1913, John Gregory Garratt

Traversea: The Journal of Transatlantic History

No abstract provided.

The Shape Of Appalachia To Come: Coal In A Transitional Economy, Randal A. Strobo

Duke Forum for Law & Social Change

No abstract provided.

Usft Institutional Repository Annual Report: Fy 2011-2012, Rebel A. Cummings-Sauls

Digital Commons @ USF Publications

In the fall of 2011, the Academic Resources Unit of Tampa Library officially launched Scholar Commons, the University of South Florida’s (USF’s) institutional repository (IR), as a virtual showcase for research and creative energies. Scholar Commons provides digital preservation and free, open access to all scholarly works produced by the USF Community. Scholar Commons will be a vehicle to drive research communication across disciplines and the globe.

This is the first annual report of the IR at USF Tampa, however this review covers the history of the IR beginning in December of 2007. This was done so that a compare …