Digital Commons Network^™

Analisis Kecelaruan Membaca Bahasa Melayu Pelajar Kelas Intervensi Awal Membaca Dan Menulis (Kia2m) / A. Jailani Bin Che Abas, Che Abas A. Jailani

Student Works (2010-2019)

The objective of this research is to study on the confusion in reading Malay language among year one pupils involve with program known as ‘Kelas Intervensi Awal Membaca dan Menulis (KIA2M).This research hopes to help teachers to plan for the group as the issue of illiteracy has been seriously attended to . The seriousness of this issue can be seen when the program is replaced with Literacy and Numeracy programs (LINUS), that is National Key Result Areas (NKRA) as a main focus by the Ministry Of Education Malaysia. A total of 22 pupils involved in this research representing 220 Year …

The Role Of Playground Games In The Syntactic Development In Sudanese Children / Abubakr Abdalgadir Mohammed Abdalla, Abubakr Abdalgadir Mohammed Abdalla

Student Works (2010-2019)

This study explores the playground games performed by Sudanese preschoolers and school going age children in an effort to determine the role of these in the syntactic development among its performers. The researcher’s own children as well as Sudanese students in some international schools in Kuala Lumpur form the population of participants in this study. The study adopts a qualitative research method and its data is collected by means of tape recording, transcribed and analyzed using the Minimalist Program which is latest development in Generative Grammar as a framework of analysis. This is done to determine foremost the grammaticality of …

The Impact Of Sign Language Exposure And Use In Deaf Children's Acquisition Of Vocabulary / Chong Vee Yee, Vee Yee Chong

Student Works (2010-2019)

This research studies the expressive and receptive skills of four Deaf children with the aim of investigating how different linguistic environment, such as the amount of input and practice, will affect their sign language acquisition. The study was conducted through observations of the subjects in the formal learning activity sessions and their regular activities in three different settings: home, church and shopping mall. Interviews were conducted with each of the subjects’ parents to gather background information regarding the linguistic input the subjects have acquired as well as the way the subjects communicate with their parents and the people around them. …

Using Mouse Embryonic Fibroblast (Mef) As Feeder Cells For Production Of Embryonic Stem Cell (Esc) Line In The Murine And Caprine / Goh Siew Ying, Siew Ying Goh

Student Works (2010-2019)

Embryonic stem cells (ESC) have unlimited potential in the field of biological sciences and regenerative medicine due to their pluripotency and ability to self-renew indefinitely. With the goal to establish, isolate and culture murine (mESC) as well as caprine (gESC) embryonic stem cells, in vivo- and in vitro-derived blastocysts were used as a source in producing mESC and gESC lines. Both mESC and gESC were cultured in vitro using mouse embryonic fibroblast (MEF) as feeder cell layer in this study. The aims of this study were: a) to compare the effects of murine strain, blastocyst stage and inner cell mass …

Immunomodulatory And Anti-Metastatic Activities Of Selected Zingiberaceae Species / Chew Ann Jee, Ann Jee Chew

Student Works (2010-2019)

No abstract provided.

A Taxonomic Revision Of Loxocarpus (Gesneriaceae) / Yao Tze Leong, Tze Leong Yao

Student Works (2010-2019)

Henckelia sect. Loxocarpus is a small taxon consisting of c. 26 taxa distributed in West Malesia. The present study is the first comprehensive taxonomic revision for all species of Loxocarpus. This study has four objectives. The first one is to provide a review of macro-morphological diversity and variation within Loxocarpus. Second, to carry out phylogenetic analyses to determine whether Loxocarpus is distinct from or is a section within Henckelia based on DNA sequences and morphological data. Third is to provide a full taxonomic revision of Loxocarpus. And last, to provide ecology and phytogeographic perspectives of Loxocarpus species and their conservation …

A Revision Of Erycibe (Convolvulaceae) In Peninsular Malaysia / Syahida Emiza Binti Suhaimi, Syahida Emiza Suhaimi

Student Works (2010-2019)

Erycibe is a big taxon consisting about 75 taxa distributed from western India and Sri Lanka across tropical and subtropical Asia to the northern tip of Queensland, Australia. The present study is focusing on the morphology and taxonomy of Erycibe in Peninsular Malaysia. The results show that Peninsular Malaysia consists of nineteen taxa. At present, seventeen taxa have scientific names with an additional two new taxa known as Erycibe. sp. A and E. sp. B. Four taxa are endemic to Peninsular Malaysia. In the taxonomic revision, a key to aid taxa identification was developed based on a combination of morphological …

Production Of Cloned-Caprine Embryos In Vitro Obtained From Interspecies Nuclear Transfer Using Bovine Cytoplast And Caprine Karyoplast / Soh Hui Hui, Hui Hui Soh

Student Works (2010-2019)

Production of cloned-caprine embryos using the intraspecies somatic cell nuclear transfer is limited by low source of caprine oocytes as the recipient cytoplast in Malaysia. Therefore, using the bovine oocytes as recipient cytoplast in interspecies somatic cell nuclear transfer is an alternative approach to produce large number of cloned-caprine embryos and subsequently offspring at a rapid rate. This study was aimed to produce cloned-caprine embryos in vitro by specifically evaluating the interspecies nuclear transfer technique such as enucleation methods, nuclear transfer methods and in vitro culture systems. Briefly, the bovine ovaries were collected from local abattoir and transported to the …

The Effects Of Hylocereus Polyrhizus And Hylocereus Undatus On Physiocochemical, Proteolysis, Exopolysaccharides Production, And Therapeutic Properties Of Yogurt / Khairul Hazmi Bin Zainoldin, Khairul Hazmi Zainoldin

Student Works (2010-2019)

Yogurt is generally recognized as a healthy and multifunctional food. It is a coagulated milk product obtained from the lactic acid fermentation by the action of Lactobacillus bulgaricus and Streptococcus thermophilus. In this present studies, dragon fruit Hylocereus polyrhizus and Hylocereus undatus extract was added in milk and their effects during yogurt fermentation were investigated. The changes in pH and Titratable Acidity (TA) were measured during fermentation and after 14 days of storage at 4°C. The yogurt extracts were subsequently analysed for their syneresis, proteolysis, peptide content, total phenolic content, antioxidant activities, inhibitory activities on α-amylase and α-glucosidase, presence of …

Phenotypic And Molecular Characterisation Of Clinical Carbapenem-Resistant Acinetobacter Baumannii / Kong Boon Hong, Boon Hong Kong

Student Works (2010-2019)

Acinetobacter baumannii is an opportunistic pathogen with increasing relevance in nosocomial infections. They cause a wide range of clinical complications, such as pneumonia, septicemia, urinary tract infection, wound infection, and meningitis, particularly in immunocompromised patients. Treatment of A. baumannii infections is often complicated by their resistance to multiple antimicrobial agents available.Carbapenem has remained as the effective antimicrobial agent for the treatment of A. baumannii infections. However, carbapenem-resistant A. baumannii is increasingly reported worldwide. In Malaysia, detailed information on the epidemiology and the mechanism of antimicrobial resistance of A. baumannii is still lacking. Hence, the objectives of this study were to …

Development And Validation Of Chromatography And Spectrometric Methods For Characterization And Impurity Profiling Of Seized Heroin For Forensic Intelligence Purposes / Chan Kar Weng, Kar Weng Chan

Student Works (2010-2019)

Heroin profiling has been undertaken to derive forensic intelligence from the seized samples and make predictions as per the criminal activities. Six major tasks were performed to collect the relevant data from 311 heroin case samples submitted in 2010. Ten visual and physical characteristics were first collected from the case samples in Task 1. Subsequently in Task 2, five types of plastic films were successfully distinguished by attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR) from the plastic packages used to contain the drug substances. This technique is rapid, robust and able to show repeatable (RSD < 2%) and reproducible (RSD < 3%) quantitative results when data normalization is used to minimize the variations arising from the poor optical contact and other uncontrolled factors. In Task 3, all the samples were analyzed by gas chromatography-mass spectrometry (GC-MS) and gas chromatography-flame ionization detection (GC-FID) to determine the levels of eight major compounds. Prior to analysis, the methods were also optimized and validated using chemical standards. The GC-FID method proved to be rapid (< 12 min), simple, precise (RSD < 5%), accurate (recoveries 99 – 102%) and robust for high throughput analysis. On the other hand, the GC-MS method is also found suitable for the profiling purpose. Furthermore, 8 links of simulated samples were analyzed following the procedures adopted by the case samples. This simulated dataset has successfully achieved eleven Normalizationselected + Standardization (Nselected + S) quotients for origin level sample classification where the clustering of case samples using principal component analysis (PCA) and hierarchical cluster analysis (HCA) operating with Ward-Manhattan is concerned. With the opium-based alkaloids, a novel tetrahedron model has also been developed to simplify the chemometric procedure involved in sample classification. Based on the purity level determined from the iii previous task, the samples were analyzed using a sample weight equivalent to 15 mg heroin base or a 650 mg constant sample weight in Task 4 for impurity profiling. This was done after 12 impurity peaks were identified and a GC-FID method was optimized and validated. The method was also demonstrated to be rapid (< 35 min), precise (RSD < 10%) and stable. Both of the sample weight approaches were statistically validated using samples of known relationships. Normalization + Standardization (N + S) was found to be the best pretreatment method and it performed particularly well in HCA operating with the Ward-Squared Euclidean and Ward-Squared Pearson. This technique was then applied to the case samples to evaluate the sample-to-sample relationships at the manufacturing level. Task 5 was carried out by quantifying trace elements. A method using inductively coupled plasma-mass spectrometry (ICP-MS) was validated using chemical standards and it proved stable for analysis. Highly variable ICP-MS data were often obtained but the intra-batch variation can be minimized via the Normalization + Fourth root (N + 4R) pretreatment method. This was verified by six batches of related samples using Ward-Squared Euclidean through HCA. Furthermore, various sources of metal contamination were also evaluated. The investigation showed that trace metals could only be used for street level sample classification. All the physical and chemical data were finally updated on a database in Task 6.

Enzyme Production Using Organic By-Products As Substrate In Solid State Fermentation / Nithiya Arumugam, Nithiya Arumugam

Student Works (2010-2019)

Brewery spent grain (BSG) which was used as the main organic material in this research is the by-product of brewing industries. Every 100 liter of beer generates 20kg of BSG. A Malaysian brewery (Carlsberg (M) Sdn. Bhd) alone generates 31,000 tonnes of BSG yearly. Almost all the BSG are being dumped into landfills, since there is no proper and separate guideline for industrial organic waste management in Malaysia. BSG materials are rich in nutrients and high in fermentable sugar as well as lignin, cellulose and hemicellulose. Dumping this high nutritional substrate into the landfills could contribute to adverse environmental impacts, …

Biological And Chemical Characterisation Of Marine Streptomycetes Active Against Selected Pathogenic Yeasts / Mumtaz Hidayahtullah Atau @ Yatau, Atau @ Yatau Mumtaz Hidayahtullah

Student Works (2010-2019)

Streptomyces spp. have been the most abundant sources of all types of antibiotics. The success of marine natural products has promoted the marine environment as a source of novel chemical diversity for drug discovery. In Malaysia, the marine actinomycetes niche remains virtually unexplored and is a promising resource for the biotechnological applications including drug discovery. Screening for antifungal activity revealed that six out of 44 putative Streptomyces spp. isolated from sponges from Tioman Island showed strong antifungal activities against Candida albicans and Schizosaccharomyces pombe. Some physiological characterisation was done to these six potential strains to facilitate the strain identification including …

The Surveillance And Resistance Status Of Aedes Mosquitoes Against Insect Growth Regulators In Malaysia / Lau Koon Weng, Koon Weng Lau

Student Works (2010-2019)

Dengue is the most important mosquito-borne disease in Malaysia and the principal vector of dengue are Aedes aegypti and Aedes albopictus. Little information was available of Insect growth regulators (IGRs) in Malaysia. This study was attempted to (1) determine the distribution and abundance of Aedes mosquitoes in multiple storey buildings in Selangor and Kuala Lumpur; (2) evaluate the susceptibility status of nationwide collected Aedes mosquitoes against IGRs; and (3) investigate the residual efficacy of IGRs in indoor and outdoor conditions. The vertical distribution and abundance of Aedes mosquitoes were determined in 4 high rise apartments located in Selangor [Kg. Baiduri …

Chemical Constituents Of Kopsia Singapurensis Ridl. And Their Antiplasmodial Activity / Noor Aimi Bt Othman @ Ghazali, Noor Aimi Othman

Student Works (2010-2019)

The chemical constituents of the Malaysian Apocynaceae Kopsia singapurensis Ridl. (KL5334) has been carried out in this study. Seven substances ; methyl sinapate (IUPAC name : (E)-methyl-3-(4-hydroxy-3,5-dimethoxyphenyl) acrylate 82, leuconolam 83, trans-2,2`-dicarboxyazobenzene dioxide 84, lonicerine 85, 15-hydroxykopsinine 86, singapurine 87 and N4-hydroxymethyl kopsinic acid 88, have been isolated using several chromatography techniques such as column chromatography and thin layer chromatography from the bark of Kopsia singapurensis Ridl.. Methyl sinapate 82 and trans-2,2`-dicarboxyazobenzene dioxide 84 are new natural compounds while singapurine 87 and N4-hydroxymethyl kopsinic acid 88 are two new indole alkaloids. The structures of the isolated compounds were elucidated by …

Antioxidant, Genoprotective And Hepatoprotective Activities Of Panus Giganteus (Berk.) Corner / Wong Wei Lun, Wei Lun Wong

Student Works (2010-2019)

Panus giganteus, a culinary and medicinal mushroom consumed by selected indigenous communities in Malaysia is currently being considered for large scale cultivation. This study was performed to investigate the medicinal potential of P. giganteus fruiting bodies and wheat grains fermented by P. giganteus including antioxidant, genoprotective and hepatoprotective properties. Ethanol extracts of P. giganteus fruiting bodies, wheat grains fermented by P. giganteus and unfermented wheat grains exhibited moderate antioxidant properties by virtue of DPPH free radical scavenging activity, reducing power, antioxidant capacity and inhibition of lipid peroxidation. The extracts also contained moderate amounts of phenolic compounds. Fruiting bodies were more …

Acute Toxicity, Antioxidant And Wound Healing Potential Of Ethanolic Extract Of Goniothalamus Umbrosus In Rats / Wasan Mj Hussain Al-Kadmi, Wasan Mj Hussain Al-Kadmi

Student Works (2010-2019)

This study was conducted to evaluate the effects of topical application of an ethanol extract of Goniothalamus umbrosus on the rate of skin wound healing closure and the histology of healed wound. The animal groups were topically treated with 0.2 ml of each vehicle (gum acacia), Intrasite gel, 100 and 200 mg/ml of ethanol leaf extract, respectively. An area of uniform wound 2.00 cm in diameter was excised from the nape of the dorsal neck of all rats with the aid of round seal using circular stamp.Macroscopically, skin wound dressed with rhizomes extract and Intrasite gel showed significantly healed earlier …

Echoes From The Hill, Southwestern Oklahoma State University Office Of Institutional Advancement And Alumni

Echoes from the Hill

No abstract provided.

Pryor Mountain Mustangs: On The Range Or On The Bring?, Monica Gokey

Graduate Student Theses, Dissertations, & Professional Papers

At the Pryor Mountain Wild Horse Range straddling the Montana-Wyoming border, the fate of a herd of mustangs rests with the Bureau of Land Management and local horse conservationists. Nationally, the two sides are generally pitted against each other in the ugly world of horse politics. But in the Pryors, advocates and government have forged a tenuous partnership. Both sides want to see healthy horses on healthy range. And there could hardly be a better herd to benefit from such a truce. The Pryor Mountain mustangs have a unique genetic heritage at stake. The horses are some of the last …

The Ecology Of Tick-Borne Relapsing Fever In Western North America, Tammi Lynne Johnson

Graduate Student Theses, Dissertations, & Professional Papers

In North America the primary cause of tick-borne relapsing fever (TBRF) is the spirochete Borrelia hermsii that is vectored by the tick Ornithodoros hermsi. Ecological investigations were combined with mathematical modeling and genetics to gain a clearer understanding of the interactions and mechanisms responsible for disease maintenance, distribution, and genetic diversity. The objectives of this research were to: 1) identify mammals associated with B. hermsii and O. hermsi by determining active infection and antibody presence, 2) develop a deterministic model to ascertain ecological and epidemiological parameters essential for disease persistence, 3) resolve the phylogeographic structure of B. hermsii and …

A Betrayed Ideal: The Problem Of Enforcement Of Eu Sex Equality Guarantees In The Cee Post-Socialist Legal Systems, Goran Selanec

SJD Dissertations

The notion of equality between men and women has, for a long time, played a significant role in the societies of Central and Eastern Europe (CEE). The ideal was particularly important during the period of “real” or “really existing” socialism in CEE. For the CEE socialist regimes, the ideal of equality was an ideological banner that supposedly demonstrated their moral superiority to the “West”. The ideal has gained new importance in recent years, when the CEE post-socialist states had to commit to the protection of the notion of equality between sexes as a condition of their membership in the European …

Enhanced Killing Effect Of Nanosecond Pulse Electric Fields On Panc1 And Jurkat Cell Lines In The Presence Of Tween 80, Gaurav Basu, Bhargava Subhash Kalluri, Ahmet Can Sabuncu, Christopher J. Osgood, Michael W. Stacey

Bioelectrics Publications

We investigated the effects of nanosecond pulse electric fields (nsPEFs) on Jurkat and PANC1 cells, which are human carcinoma cell lines, in the presence of Tween 80 (T80) at a concentration of 0.18% and demonstarted an enhanced killing effect. We used two biological assays to determine cell viability after exposing cells to nsPEFs in the presence of T80 and observed a significant increase in the killing effect of nsPEFs. We did not see a toxic effect of T80 when cells were exposed to surfactant alone. However, we saw a synergistic effect when cells exposed to T80 were combined with the …

Probing Nanoparticle Interactions In Cell Culture Media, Ahmet C. Sabuncu, Janna Grubbs, Shizhi Qian, Tarek M. Abdel-Fattah, Michael W. Stacey, Ali Beskok

Bioelectrics Publications

Nanoparticle research is often performed in vitro with little emphasis on the potential role of cell culture medium. In this study, gold nanoparticle interactions with cell culture medium and two cancer cell lines (human T-cell leukemia Jurkat and human pancreatic carcinoma PANC1) were investigated. Gold nanoparticles of 10, 25, 50, and 100 nm in diameter at fixed mass concentration were tested. Size distributions and zeta potentials of gold nanoparticles suspended in deionized (DI) water and Dulbecco's Modified Eagle's Media (DMEM) supplemented with fetal calf serum (FCS) were measured using dynamic light scattering (DLS) technique. In DI water, particle size distributions …

Honors Research Symposium Program [2012], University Honors Program Students And Staff

Honors College: News & Publications (2008-2020)

No abstract provided.

Honors @ Georgia Southern, University Honors Program Students And Staff, Georgia Southern University

Honors College: News & Publications (2008-2020)

• Albania
• Two Generations
• 30th Anniversary
• Honors Annual Report
• Honors in Action

Inhibition Of Voltage-Gated Na+ Current By Nanosecond Pulsed Electric Field (Nspef) Is Not Mediated By Na+ Influx Or Ca²+ Signaling, Vasyl Nesin, Andrei G. Pakhomov

Bioelectrics Publications

In earlier studies, we found that permeabilization of mammalian cells with nsPEF was accompanied by prolonged inhibition of voltage-gated (VG) currents through the plasma membrane. This study explored if the inhibition of VG Na⁺ current (I_Na) resulted from (i) reduction of the transmembrane Na⁺ gradient due to its influx via nsPEF-opened pores, and/or (ii) downregulation of the VG channels by a Ca²⁺ -dependent mechanism. We found that a single 300?ns electric pulse at 1.65.3?kV/cm triggered sustained Na⁺ influx in exposed NG108 cells and in primary chromaffin cells, as detected by increased fluorescence of a …

Electric Field Exposure Triggers And Guides Formation Of Pseudopod-Like Blebs In U937 Monocytes, Mikhail A. Rassokhin, Andrei G. Pakhomov

Bioelectrics Publications

We describe a new phenomenon of anodotropic pseudopod-like blebbing in U937 cells stimulated by nanosecond pulsed electric field (nsPEF). In contrast to "regular," round-shaped blebs, which are often seen in response to cell damage, pseudopod-like blebs (PLBs) formed as longitudinal membrane protrusions toward anode. PLB length could exceed the cell diameter in 2 min of exposure to 60-ns, 10-kV/cm pulses delivered at 10-20 Hz. Both PLBs and round-shaped nsPEF-induced blebs could be efficiently inhibited by partial isosmotic replacement of bath NaCl for a larger solute (sucrose), thereby pointing to the colloid-osmotic water uptake as the principal driving force for bleb …

The Use Of An In Vitro 3d Melanoma Model To Predict In Vivo Plasmid Transfection Using Electroporation, Benadette Marrero, Richard Heller

Bioelectrics Publications

A large-scale in vitro 3D tumor model was generated to evaluate gene delivery procedures in vivo. This 3D tumor model consists of a "tissue-like" spheroid that provides a micro-environment supportive of melanoma proliferation, allowing cells to behave similarly to cells in vivo. This functional spheroid measures approximately 1 cm in diameter and can be used to effectively evaluate plasmid transfection when testing various electroporation (EP) electrode applicators. In this study, we identified EP conditions that efficiently transfect green fluorescent protein (GFP) and interleukin 15 (IL-15) plasmids into tumor cells residing in the 3D construct. We found that plasmids …

Asymmetry Of The Humerus: The Influence Of Handedness On The Deltoid Tuberosity And Possible Implications For Osteometric Sorting, Carrie Brady Legarde

Graduate Student Theses, Dissertations, & Professional Papers

The identification of individuals from commingled human remains can be a difficult task. Osteometric sorting is often utilized for these purposes since it sorts remains based on size. This research investigates whether asymmetry of the humerus is present, if it is due to an individual’s hand preference, and if this asymmetry can adversely affect the osteometric sorting method. The osteometric sorting formulae for pair-matching was used to classify individuals as asymmetric when elements were considered significantly different in size. Significant asymmetry was seen in multiple measurements and the asymmetry could be due to handedness. This relationship appeared more consistent with …

Amur Tiger Predation And Energetic Requirements In The Russian Far East: New Insights From Global Positioning System Collars, Clayton Steele Miller

Graduate Student Theses, Dissertations, & Professional Papers

The IUCN Red List has classified all subspecies of tigers (Panthera tigris) as endangered with prey depletion being widely recognized as one of the primary drivers of tiger declines. Due to substantial energetic requirements, tigers can only survive and reproduce in areas with healthy prey populations. This may be particularly important for Amur tigers (P. t. altaica) in the Russian Far East, living at the northern limits and with the lowest prey densities of any tiger population. Few studies have been able to rigorously investigate annual prey requirements for any tiger population. We deployed Global Positioning System (GPS) collars on …