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2012

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Articles 16681 - 16710 of 22929

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Genome Sequence Of Multidrug-Resistant Escherichia Coli Ec302/04, Isolated From A Human Tracheal Aspirate, Wing Sze Ho, Han Ming Gan, Kien Pong Yap, Ganeswrie Balan, Chew Chieng Yeo, Kwai Lin Thong Jan 2012

Genome Sequence Of Multidrug-Resistant Escherichia Coli Ec302/04, Isolated From A Human Tracheal Aspirate, Wing Sze Ho, Han Ming Gan, Kien Pong Yap, Ganeswrie Balan, Chew Chieng Yeo, Kwai Lin Thong

Research Publications (2011 to 2015)

Escherichia coli is an important etiologic agent of lower respiratory tract infections (LRTI). Multidrug-resistant E. coli EC302/04 was isolated from a tracheal aspirate, and its genome sequence is expected to provide insights into antimicrobial resistance as well as adaptive and virulence mechanisms of E. coli involved in LRTI.

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Genotypic And Phenotypic Characterization Of Chikungunya Virus Of Different Genotypes From Malaysia, I.C. Sam, S.K. Loong, J.C. Michael, C.L. Chua, W.Y.W. Sulaiman, I. Vythilingam, S.Y. Chan, C.W. Chiam, Y.S. Yeong, Sazaly Abubakar, Yoke Fun Chan Jan 2012

Genotypic And Phenotypic Characterization Of Chikungunya Virus Of Different Genotypes From Malaysia, I.C. Sam, S.K. Loong, J.C. Michael, C.L. Chua, W.Y.W. Sulaiman, I. Vythilingam, S.Y. Chan, C.W. Chiam, Y.S. Yeong, Sazaly Abubakar, Yoke Fun Chan

Research Publications (2011 to 2015)

Background: Mosquito-borne Chikungunya virus (CHIKV) has recently re-emerged globally. The epidemic East/Central/South African (ECSA) strains have spread for the first time to Asia, which previously only had endemic Asian strains. In Malaysia, the ECSA strain caused an extensive nationwide outbreak in 2008, while the Asian strains only caused limited outbreaks prior to this. To gain insight into these observed epidemiological differences, we compared genotypic and phenotypic characteristics of CHIKV of Asian and ECSA genotypes isolated in Malaysia. Methods and Findings: CHIKV of Asian and ECSA genotypes were isolated from patients during outbreaks in Bagan Panchor in 2006, and Johor in …

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Glucan-Rich Polysaccharides From Pleurotus Sajor-Caju (Fr.) Singer Prevents Glucose Intolerance, Insulin Resistance And Inflammation In C57bl/6j Mice Fed A High-Fat Diet, G. Kanagasabapathy, U.R. Kuppusamy, S.N. Abd Malek, M.A. Abdulla, K.H. Chua, V. Sabaratnam Jan 2012

Glucan-Rich Polysaccharides From Pleurotus Sajor-Caju (Fr.) Singer Prevents Glucose Intolerance, Insulin Resistance And Inflammation In C57bl/6j Mice Fed A High-Fat Diet, G. Kanagasabapathy, U.R. Kuppusamy, S.N. Abd Malek, M.A. Abdulla, K.H. Chua, V. Sabaratnam

Research Publications (2011 to 2015)

Background: Pleurotus sajor-caju (P. sajor-caju) has been extremely useful in the prevention of diabetes mellitus due to its low fat and high soluble fiber content for thousands of years. Insulin resistance is a key component in the development of diabetes mellitus which is caused by inflammation. In this study, we aimed to investigate the in vivo efficacy of glucan-rich polysaccharide of P. sajor-caju (GE) against diabetes mellitus and inflammation in C57BL/6J mice fed a high-fat diet. Methods: Diabetes was induced in C57BL/6J mice by feeding a high-fat diet. The mice were randomly assigned to 7 groups (n=6 per group). The …

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Hepatoprotective Effects Of Panus Giganteus (Berk.) Corner Against Thioacetamide- (Taa-) Induced Liver Injury In Rats, Wei Lun Wong, Mahmood Ameen Abdulla, Kek Heng Chua, Umah Rani Kuppusamy, Yee Shin Tan, Vikineswary Sabaratnam Jan 2012

Hepatoprotective Effects Of Panus Giganteus (Berk.) Corner Against Thioacetamide- (Taa-) Induced Liver Injury In Rats, Wei Lun Wong, Mahmood Ameen Abdulla, Kek Heng Chua, Umah Rani Kuppusamy, Yee Shin Tan, Vikineswary Sabaratnam

Research Publications (2011 to 2015)

Panus giganteus, a culinary and medicinal mushroom consumed by selected indigenous communities in Malaysia, is currently being considered for large scale cultivation. This study was undertaken to investigate the hepatoprotective effects of P. giganteus against thioacetamide- (TAA-) induced liver injury in Sprague-Dawley rats. The rats were injected intraperitoneally with TAA thrice weekly and were orally administered freeze-dried fruiting bodies of P. giganteus (0.5 or 1 g/kg) daily for two months, while control rats were given vehicle or P. giganteus only. After 60 days, rats administered with P. giganteus showed lower liver body weight ratio, restored levels of serum liver biomarkers …

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Identification Of Potential Complementary Serum Biomarkers To Differentiate Prostate Cancer From Benign Prostatic Hyperplasia Using Gel- And Lectin-Based Proteomics Analyses, J.J. Jayapalan, K.L. Ng, A.H.A. Razack, Onn Haji Hashim Jan 2012

Identification Of Potential Complementary Serum Biomarkers To Differentiate Prostate Cancer From Benign Prostatic Hyperplasia Using Gel- And Lectin-Based Proteomics Analyses, J.J. Jayapalan, K.L. Ng, A.H.A. Razack, Onn Haji Hashim

Research Publications (2011 to 2015)

Diagnosis of prostate cancer (PCa) is currently much reliant on the invasive and time-consuming transrectal ultrasound-guided biopsy of the prostate gland, particularly in light of the inefficient use of prostate-specific antigen as its biomarker. In the present study, we have profiled the sera of patients with PCa and benign prostatic hyperplasia (BPH) using the gel- and lectin-based proteomics methods and demonstrated the significant differential expression of apolipoprotein AII, complement C3 beta chain fragment, inter-alpha-trypsin inhibitor heavy chain 4 fragment, transthyretin, alpha-1-antitrypsin, and high molecular weight kininogen (light chain) between the two groups of patientsâ�� samples. Our data are suggestive of …

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Igf2bp2 Alternative Variants Associated With Glutamic Acid Decarboxylase Antibodies Negative Diabetes In Malaysian Subjects, S.D. Salem, R. Saif-Ali, I.S. Ismail, Z. Al-Hamodi, R. Poh, S. Muniandy Jan 2012

Igf2bp2 Alternative Variants Associated With Glutamic Acid Decarboxylase Antibodies Negative Diabetes In Malaysian Subjects, S.D. Salem, R. Saif-Ali, I.S. Ismail, Z. Al-Hamodi, R. Poh, S. Muniandy

Research Publications (2011 to 2015)

Background: The association of Insulin-like growth factor 2 mRNA-binding protein 2 (IGF2BP2) common variants (rs4402960 and rs1470579) with type 2 diabetes (T2D) has been performed in different populations. The aim of this study was to evaluate the association of alternative variants of IGF2BP2; rs6777038, rs16860234 and rs7651090 with glutamic acid decarboxylase antibodies (GADA) negative diabetes in Malaysian Subjects. Methods/Principal Findings: IGF2BP2; rs6777038, rs16860234 and rs7651090 single nucleotide polymorphisms (SNPs) were genotyped in 1107 GADA negative diabetic patients and 620 control subjects of Asian from Malaysia. The additive genetic model adjusted for age, race, gender and BMI showed that alternative variants; …

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In Vitro Plant Regeneration, Antioxidant And Antibacterial Studies On Broccoli, Brassica Oleracea Var. Italica, R. Farzinebrahimi, R.M. Taha, M. Fadainasaband, S. Mokhtari Jan 2012

In Vitro Plant Regeneration, Antioxidant And Antibacterial Studies On Broccoli, Brassica Oleracea Var. Italica, R. Farzinebrahimi, R.M. Taha, M. Fadainasaband, S. Mokhtari

Research Publications (2011 to 2015)

Leaf and shoot tip explant of Brassica oleracea var. italica were inoculated on Murashige and Skoog (MS) basal medium supplemented with various concentrations of (BAP) and (IBA), alone and in combinations to achieve plant regeneration. Subsequently, antioxidant and antibacterial activities were determined from In vitro and In vivo plant parts. The highest mean numbers of shoot was observed on combinations of 1 mg l(-1) and 1.5 mg l(-1) of BAP and IBA, 9.49 and 8.69, respectively. The highest number of shoot produced (11.55) per shoot tip explant was recorded on 2.5 mg l(-1) BAP. In addition, highest percentage of roots …

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Induction Of Different Types Of Callus And Somatic Embryogenesis In Various Explants Of Sainfoin (Onobrychis Sativa), S. Mohajer, R.M. Taha, A. Khorasani, J.S. Yaacob Jan 2012

Induction Of Different Types Of Callus And Somatic Embryogenesis In Various Explants Of Sainfoin (Onobrychis Sativa), S. Mohajer, R.M. Taha, A. Khorasani, J.S. Yaacob

Research Publications (2011 to 2015)

To explore the potential for in vitro rapid regeneration of Sainfoin (Onobrychis sativa), different concentrations of 6-Benzylaminopurine (BAP), 1-naphthaleneacetic acid (NAA) and BAP combined with Indole-3-butyric acid (IBA) were evaluated for their effects on the induction of somatic embryos from leaf, stem and root explants. Different explants were cultured on MS medium supplemented with various concentrations (0, 0.5, 1, 1.5, 2, 2.5 and 3 mg/l) of each kind of hormone. Callus induction percentage, fresh weight, color and texture of the callus were assessed after 11 and 22 days of culture. The optimum medium for the proliferation of embryogenic calli from …

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Induction Of Mitochondria-Mediated Apoptosis In Ca Ski Human Cervical Cncer Cells Triggered By Mollic Acid Arabinoside Isolated From Leea Indica, Y.H. Wong, H. Abdul Kadir Jan 2012

Induction Of Mitochondria-Mediated Apoptosis In Ca Ski Human Cervical Cncer Cells Triggered By Mollic Acid Arabinoside Isolated From Leea Indica, Y.H. Wong, H. Abdul Kadir

Research Publications (2011 to 2015)

Leea indica is a medicinal plant traditionally used to treat cancer. Through bioassay-guided approach, we isolated mollic acid arabinoside (MAA), for the first time from Leea indica. Here, we present the apoptosis-inducing effect of MAA on Ca Ski cervical cancer cells. Based on DAPI staining, MAA-treated cells manifested nuclear shrinkage, condensation, and fragmentation. We further confirmed the fragmentation of DNA using TUNEL assay. During early apoptosis, MAA caused the perturbation of plasma membrane through externalization of PS, followed by the formation of apoptotic blebs. Prior to these events, MAA triggered rapid dissipation of the mitochondrial membrane potential. In the upstream, …

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Influences Of Environmental And Operational Factors On Dark Fermentative Hydrogen Production: A Review, P. Mohammadi, S. Ibrahim, M.S.M. Annuar, S. Ghafari, S. Vikineswary, A.A. Zinatizadeh Jan 2012

Influences Of Environmental And Operational Factors On Dark Fermentative Hydrogen Production: A Review, P. Mohammadi, S. Ibrahim, M.S.M. Annuar, S. Ghafari, S. Vikineswary, A.A. Zinatizadeh

Research Publications (2011 to 2015)

Hydrogen (H2) is one of renewable energy sources known for its non-polluting and environmentally friendly nature, as its end combustion product is water (H2O). The biological production of H2 is a less energy intensive alternative where processes can be operated at ambient temperature and pressure. Dark fermentation by bacterial biomass is one of multitude of approaches to produce hydrogen which is known as the cleanest renewable energy and is thus receiving increasing attention worldwide. The present study briefly reviews the biohydrogen production process with special attention on the effects of several environmental and operational factors towards the process. Factors such …

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Inhibition Of Dengue Ns2b-Ns3 Protease And Viral Replication In Vero Cells By Recombinant Retrocyclin-1, H.A. Rothan, H.C. Han, T.S. Ramasamy, S. Othman, N.A. Rahman, Rohana Yusof Jan 2012

Inhibition Of Dengue Ns2b-Ns3 Protease And Viral Replication In Vero Cells By Recombinant Retrocyclin-1, H.A. Rothan, H.C. Han, T.S. Ramasamy, S. Othman, N.A. Rahman, Rohana Yusof

Research Publications (2011 to 2015)

Background; Global resurgence of dengue virus infections in many of the tropical and subtropical countries is a major concern. Therefore, there is an urgent need for the development of successful drugs that are both economical and offer a long-lasting protection. The viral NS2B-NS3 serine protease (NS2B-NS3pro) is a promising target for the development of drug-like inhibitors, which are not available at the moment. In this study, we report retrocyclin-1 (RC-1) production in E. coli as a recombinant peptide to test against dengue NS2B-NS3pro. Methods: Dengue NS2B-NS3pro was produced as a recombinant single chain protein in E. coli and purified by …

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Inhibition Of Quorum Sensing-Controlled Virulence Factor Production In Pseudomonas Aeruginosa Pao1 By Ayurveda Spice Clove (Syzygium Aromaticum) Bud Extract, T. Krishnan, W.F. Yin, Kok Gan Chan Jan 2012

Inhibition Of Quorum Sensing-Controlled Virulence Factor Production In Pseudomonas Aeruginosa Pao1 By Ayurveda Spice Clove (Syzygium Aromaticum) Bud Extract, T. Krishnan, W.F. Yin, Kok Gan Chan

Research Publications (2011 to 2015)

Quorum sensing controls the virulence determinants in most proteobacteria. In this work, the hexane, chloroform and methanol extracts of an Ayurveda spice, namely clove (Syzygium aromaticum), shown anti-quorum sensing activity. Hexane and methanol extracts of clove inhibited the response of C. violaceum CV026 to exogenously supplied N-hexanoylhomoserine lactone, in turn preventing violacein production. Chloroform and methanol extracts of clove significantly reduced bioluminescence production by E. coli [pSB1075] grown in the presence of N-(3-oxododecanoyl)-l-homoserine lactone. We demonstrated that clove extract inhibited quorum sensing-regulated phenotypes in Pseudomonas aeruginosa PA01, including expression of lecA::lux (by hexane extract), swarming (maximum inhibition by methanol extract), …

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Investigation Of Electromyographic Activity During Salat And Stretching Exercise, F. Ibrahim, S.A. Ahmad Jan 2012

Investigation Of Electromyographic Activity During Salat And Stretching Exercise, F. Ibrahim, S.A. Ahmad

Research Publications (2011 to 2015)

Salat is a formal prayer practice by all Muslims. When performing salat, it involves with several movements namely standing, takbeer, bowing, prostration and sitting. The study was to quantify and compares the muscle activity (surface electromyographic signal) during Salat and stretching exercise. Three main movements in Salat (takbeer, bowing and prostration) and similar stretching exercises to the Salat movements were investigated. Thirty one male and female volunteers were participated in this study. The activity of the four investigated muscles: biceps brachii, triceps brachii, pectoralis major and upper trapezius were recorded during salat and stretching movements. The recorded signals were then …

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Investigation Of Toxin Genes Among Methicillin-Resistant Staphylococcus Aureus Strains Isolated From A Tertiary Hospital In Malaysia, K.T. Lim, Y.A. Hanifah, M.Y. Mohd Yusof, Kwai Lin Thong Jan 2012

Investigation Of Toxin Genes Among Methicillin-Resistant Staphylococcus Aureus Strains Isolated From A Tertiary Hospital In Malaysia, K.T. Lim, Y.A. Hanifah, M.Y. Mohd Yusof, Kwai Lin Thong

Research Publications (2011 to 2015)

Staphylococcus aureus is a persistent human pathogen responsible for a variety of infections ranging from soft-tissue infections to bacteremia. It produces a variety of virulence factors which are responsible for specific acute staphylococcal toxaemia syndromes. The objective of this study was to determine the prevalence of a repertoire of toxin genes among Malaysian MRSA strains and their genetic diversity by PCR-RFLP of coa gene. One hundred eighty-eight strains (2003, 2004, 2007 and 2008) of methicillin-resistant S. aureus (MRSA) were screened for 20 genes encoding for extracellular virulence determinant (sea, seb, sec, sed, see, seg, seh, sei, sej, tst, eta, etb, …

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Ipomoea Aquatica Extract Shows Protective Action Against Thioacetamide-Induced Hepatotoxicity, M.A. Abdullah, S.S. Alkiyumi, A.S. Alrashdi, S.M. Salama, S.I. Abdelwahab, A.H.A. Hadi Jan 2012

Ipomoea Aquatica Extract Shows Protective Action Against Thioacetamide-Induced Hepatotoxicity, M.A. Abdullah, S.S. Alkiyumi, A.S. Alrashdi, S.M. Salama, S.I. Abdelwahab, A.H.A. Hadi

Research Publications (2011 to 2015)

In the Indian system of traditional medicine (Ayurveda) it is recommended to consume Ipomoea aquatica to mitigate disorders like jaundice. In this study, the protective effects of ethanol extract of I. aquatica against liver damage were evaluated in thioacetamide (TAA)-induced chronic hepatotoxicity in rats. There was no sign of toxicity in the acute toxicity study, in which Sprague-Dawley (SD) rats were orally fed with I. aquatica (250 and 500 mg/kg) for two months along with administration of TAA (i.p injection 200 mg/kg three times a week for two months). The results showed that the treatment of I. aquatica significantly lowered …

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Isolation And Identification Of Myo-Inositol Crystals From Dragon Fruit (Hylocereus Polyrhizus), O.P.S. Rebecca, A.N. Boyce, C. Somasundram Jan 2012

Isolation And Identification Of Myo-Inositol Crystals From Dragon Fruit (Hylocereus Polyrhizus), O.P.S. Rebecca, A.N. Boyce, C. Somasundram

Research Publications (2011 to 2015)

Crystals isolated from Hylocereus polyrhizus were analyzed using four different approaches-X-ray Crystallography, High Performance Liquid Chromatography (HPLC), Liquid Chromatography Tandem Mass Spectrometry (LC-MS/ MS) and Nuclear Magnetic Resonance (NMR) and identified as myo-inositol. The X-ray crystallography analysis showed that the unit-cell parameters were: a = 6.6226 (3) angstrom, b = 12.0462 (5) angstrom, c = 18.8942 (8) angstrom, alpha = 90.00, beta = 93.98, delta = 90.00. The purity of the crystals were checked using HPLC, whereupon a clean single peak was obtained at 4.8 min with a peak area of 41232 mu V*s. The LC-MS/ MS technique, which is …

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Lack Of Evidence For Intermolecular Epistatic Interactions Between Adiponectin And Resistin Gene Polymorphisms In Malaysian Male Subjects, C.H. Lau, S. Muniandy Jan 2012

Lack Of Evidence For Intermolecular Epistatic Interactions Between Adiponectin And Resistin Gene Polymorphisms In Malaysian Male Subjects, C.H. Lau, S. Muniandy

Research Publications (2011 to 2015)

Epistasis (gene-gene interaction) is a ubiquitous component of the genetic architecture of complex traits such as susceptibility to common human diseases. Given the strong negative correlation between circulating adiponectin and resistin levels, the potential intermolecular epistatic interactions between ADIPOQ (SNP+45T > G, SNP+276G > T, SNP+639T > C and SNP+1212A > G) and RETN (SNP-420C > G and SNP+299G > A) gene polymorphisms in the genetic risk underlying type 2 diabetes (T2DM) and metabolic syndrome (MS) were assessed. The potential mutual influence of the ADIPOQ and RETN genes on their adipokine levels was also examined. The rare homozygous genotype (risk alleles) of SNP-420C > G at the …

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Life Cycle Assessment For Solid Waste Disposal Options In Malaysia, S. Saheri, M.A. Mir, N.E.A. Basri, N.Z. Mahmood, R.A. Begum Jan 2012

Life Cycle Assessment For Solid Waste Disposal Options In Malaysia, S. Saheri, M.A. Mir, N.E.A. Basri, N.Z. Mahmood, R.A. Begum

Research Publications (2011 to 2015)

The largest percentage of MSW in Malaysia is contributed by the state of Selangor, with a 3,923 tons generated daily. Therefore, the aim of this study is to consider the current condition of the open dumps and sanitary landfills in Selangor from an environmental point of view. Moreover, the local authorities of Malaysia prefer to use landfills that have no liners, biogas capture, and many others as a method of getting rid of collected waste. In Malaysia, this is the first time life cycle assessment (LCA) is being used. In this study, LCA is used to weigh up different treatment …

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Life Cycle Impact Assessment (Lcia) Using The Ecological Scarcity (Ecopoints) Method: A Potential Impact Analysis To Potable Water Production, A.H. Sharaai, Noor Zalina Mahmood, A.H. Sulaiman Jan 2012

Life Cycle Impact Assessment (Lcia) Using The Ecological Scarcity (Ecopoints) Method: A Potential Impact Analysis To Potable Water Production, A.H. Sharaai, Noor Zalina Mahmood, A.H. Sulaiman

Research Publications (2011 to 2015)

Life cycle assessment (LCA) is a method use to analyze a product or a service from the beginning of the process where it is extracted until it is not useful anymore or it is known as cradle-to-grave analysis. LCA analysis includes the inventory collecting all types of emission and waste. After it is done, the inventory will be interpreted to the environmental impacts in life cycle impact assessment (LCIA). Two LCIA methods identified were "midpoint and endpoint" approaches. The ecological scarcity (ecopoints) is an LCIA method using "midpoint" approach. From the analysis to both life cycle stages, analysis for potable …

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Lignosus Rhinocerus (Cooke) Ryvarden: A Medicinal Mushroom That Stimulates Neurite Outgrowth In Pc-12 Cells, L.F. Eik, M. Naidu, P. David, K.H. Wong, Y.S. Tan, V. Sabaratnam Jan 2012

Lignosus Rhinocerus (Cooke) Ryvarden: A Medicinal Mushroom That Stimulates Neurite Outgrowth In Pc-12 Cells, L.F. Eik, M. Naidu, P. David, K.H. Wong, Y.S. Tan, V. Sabaratnam

Research Publications (2011 to 2015)

A national treasure mushroom, Lignosus rhinocerus, has been used to treat variety of ailments by local and indigenous communities in Malaysia. The aim of this study was to investigate the potential of the most valuable part of L. rhinocerus, the sclerotium, on neurite outgrowth activity by using PC-12Adh cell line. Differentiated cells with one thin extension at least double the length of the cell diameter were scored positive. Our results showed that aqueous sclerotium L. rhinocerus extract induced neurite outgrowths of 24.4% and 42.1% at 20 μg/mL (w/v) of aqueous extract alone and a combination of 20 μg/mL (w/v) aqueous …

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Mechanisms Of Gastroprotective Effects Of Ethanolic Leaf Extract Of Jasminum Sambac Against Hcl/Ethanol-Induced Gastric Mucosal Injury In Rats, Mahmood Ameen Abdulla, A.S. Alrashdi, S.M. Salama, S.S. Alkiyumi, A.H.A. Hadi, S.I. Abdelwahab, M.M. Taha, J. Hussiani, N. Asykin Jan 2012

Mechanisms Of Gastroprotective Effects Of Ethanolic Leaf Extract Of Jasminum Sambac Against Hcl/Ethanol-Induced Gastric Mucosal Injury In Rats, Mahmood Ameen Abdulla, A.S. Alrashdi, S.M. Salama, S.S. Alkiyumi, A.H.A. Hadi, S.I. Abdelwahab, M.M. Taha, J. Hussiani, N. Asykin

Research Publications (2011 to 2015)

Jasminum sambac is used in folk medicine as the treatment of many diseases. The aim of the present investigation is to evaluate the gastroprotective effects of ethanolic extracts of J. sambac leaves against acidified ethanol-induced gastric ulcers in rats. Seven groups of rats were orally pre-treated with carboxymethylcellulose (CMC) as normal group, CMC as ulcer group, 20 mg/kg of omeprazole as positive group, 62.5, 125, 250, and 500 mg/kg of extract as the experimental groups, respectively. An hour later, CMC was given orally to normal group and acidified ethanol solution was given orally to the ulcer control, positive control, and …

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Molecular Characterization Of Clinical Isolates Of Aeromonas Species From Malaysia, S.D. Puthucheary, S.M. Puah, K.H. Chua Jan 2012

Molecular Characterization Of Clinical Isolates Of Aeromonas Species From Malaysia, S.D. Puthucheary, S.M. Puah, K.H. Chua

Research Publications (2011 to 2015)

BACKGROUND: Aeromonas species are common inhabitants of aquatic environments giving rise to infections in both fish and humans. Identification of aeromonads to the species level is problematic and complex due to their phenotypic and genotypic heterogeneity. METHODOLOGY/PRINCIPAL FINDINGS: Aeromonas hydrophila or Aeromonas sp were genetically re-identified using a combination of previously published methods targeting GCAT, 16S rDNA and rpoD genes. Characterization based on the genus specific GCAT-PCR showed that 94 (96%) of the 98 strains belonged to the genus Aeromonas. Considering the patterns obtained for the 94 isolates with the 16S rDNA-RFLP identification method, 3 clusters were recognised, i.e. A. …

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Molecular Investigation Of Virulence Determinants Between A Virulent Clinical Strain And An Attenuated Strain Of Burkholderia Pseudomallei, S.D. Puthucheary, S.M. Puah, H.C. Chai, Kwai Lin Thong, K.H. Chua Jan 2012

Molecular Investigation Of Virulence Determinants Between A Virulent Clinical Strain And An Attenuated Strain Of Burkholderia Pseudomallei, S.D. Puthucheary, S.M. Puah, H.C. Chai, Kwai Lin Thong, K.H. Chua

Research Publications (2011 to 2015)

No abstract provided.

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Osteogenic Genes Related To The Canonic Wnt Pathway Are Down-Regulated In Ameloblastoma, G.A. Sathi, H. Tsujigiwa, S. Ito, C.H. Siar, N. Katase, R. Tamamura, H. Harada, H. Nagatsuka Jan 2012

Osteogenic Genes Related To The Canonic Wnt Pathway Are Down-Regulated In Ameloblastoma, G.A. Sathi, H. Tsujigiwa, S. Ito, C.H. Siar, N. Katase, R. Tamamura, H. Harada, H. Nagatsuka

Research Publications (2011 to 2015)

Objective. The aim of this study was to determine the expression of essential osteogenic genes related to the canonic WNT pathway, i.e., WDR5, sFRP-2, and their downstream genes, in ameloblastoma and to clarify their biologic impact on this neoplasm. Study Design. Forty-six paraffin-embedded ameloblastoma samples and ameloblastic (AM-1) and preosteoblastic (KUSA/A1) cell lines were used. Immunohistochemistry, Western blot, reverse-transcription polymerase chain reaction, and alkaline phosphatase (ALP) activity assay were performed. Results. WDR5, essential for osteoblast differentiation and canonic WNT pathway activation, was negative in most ameloblastoma cases and weakly expressed in AM-1 cells. Conversely, sFRP-2s was overexpressed. RUNX2 and C-MYC, …

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Outbreak-Associated Vibrio Cholerae Genotypes With Identical Pulsotypes, Malaysia, 2009, C.S.J. Teh, Z. Suhaili, K.T. Lim, M.A. Khamaruddin, F. Yahya, M.H. Sajili, C.C. Yeo, Kwai Lin Thong Jan 2012

Outbreak-Associated Vibrio Cholerae Genotypes With Identical Pulsotypes, Malaysia, 2009, C.S.J. Teh, Z. Suhaili, K.T. Lim, M.A. Khamaruddin, F. Yahya, M.H. Sajili, C.C. Yeo, Kwai Lin Thong

Research Publications (2011 to 2015)

A cholera outbreak in Terengganu, Malaysia, in November 2009 was caused by 2 El Tor Vibrio cholerae variants resistant to typical antimicrobial drugs. Evidence of replacement of treatable V. cholerae infection in the region with antimicrobial-resistant strains calls for increased surveillance and prevention measures.

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Oxyspiruriasis In Zoo Birds, S. Vellayan, J. Jeffery, P. Oothuman, M. Zahedi, M. Krishnasamy, S. Paramaswaran, M. Rohela, N.M. Abdul-Aziz Jan 2012

Oxyspiruriasis In Zoo Birds, S. Vellayan, J. Jeffery, P. Oothuman, M. Zahedi, M. Krishnasamy, S. Paramaswaran, M. Rohela, N.M. Abdul-Aziz

Research Publications (2011 to 2015)

Oxyspiruriasis caused by the bird eyeworm, Oxyspirura mansoni, a thelaziid nematode, in three species of pheasants, 3 Chrysolophus pictus (golden pheasant), 7 Lophura nycthemera (silver pheasant) and 9 Phasianus colchicus (common pheasant) in Zoo Negara Malaysia are reported. Birds with the disease were treated with a solution of 0.5 iodine or 0.5 lysol. Antistress powder for 4 days in water and non-strep vitamin powder in water was also provided. Control measures included removal of the cockroach intermediate host, Pycnoscelus surinamensis (Surinam cockroach) from the vicinity of the birds. The golden pheasant is a new host for O. mansoni in peninsular …

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Particle Swarm Optimization Techniques For Optimal Location And Sizing Of Thyristor Controlled Series Capacitor, S.A. Jumaat, I. Musirin, M.M. Othman, Hazlie Mokhlis Jan 2012

Particle Swarm Optimization Techniques For Optimal Location And Sizing Of Thyristor Controlled Series Capacitor, S.A. Jumaat, I. Musirin, M.M. Othman, Hazlie Mokhlis

Research Publications (2011 to 2015)

One of the disturbances experienced by the power system is increase in loading condition, which often led the system to no longer remains in secure operating region. When the power system is exposed to any kind of time delay and inaccessibility of control scheme, system may become inconsistent leading to uncontrolled condition. Under this condition, the main purpose of the operator is to execute control actions to get the system back into the secure operating regions. Flexible AC transmission system (FACTS) device is one of the devices, which can be inserting to control power system stability improvement. This paper describes …

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Peripheral Pdlim5 Expression In Bipolar Disorder And The Effect Of Olanzapine Administration, M.A. Zain, S.N. Jahan, G.P. Reynolds, N.Z. Zainal, S. Kanagasundram, Z. Mohamed Jan 2012

Peripheral Pdlim5 Expression In Bipolar Disorder And The Effect Of Olanzapine Administration, M.A. Zain, S.N. Jahan, G.P. Reynolds, N.Z. Zainal, S. Kanagasundram, Z. Mohamed

Research Publications (2011 to 2015)

Background: One of the genes suggested to play an important role in the pathophysiology of bipolar disorder (BPD) is PDLIM5, which encodes LIM domain protein. Our main objective was to examine the effect of olanzapine treatment on PDLIM5 mRNA expression in the peripheral blood leukocytes of BPD patients.Methods: We measured the expression of PDLIM5 mRNA from 16 patients with BPD Type I after 0, 4, and 8 weeks of treatment with olanzapine using quantitative real-time PCR. The Young Mania Rating Scale was used to evaluate the severity of manic symptoms in BPD patients. We also compared PDLIM5 mRNA expression in …

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Pharmaceutical Care Issues Encountered By Post‐Menopausal Osteoporotic Women Prescribed Bisphosphonates, Pauline Siew Mei Lai, Siew Siang Chua, S.P. Chan Jan 2012

Pharmaceutical Care Issues Encountered By Post‐Menopausal Osteoporotic Women Prescribed Bisphosphonates, Pauline Siew Mei Lai, Siew Siang Chua, S.P. Chan

Research Publications (2011 to 2015)

WHAT IS KNOWN AND OBJECTIVE: Pharmacists have been involved in providing comprehensive interventions to osteoporosis patients, but pharmaceutical care issues (PCIs) encountered during such interventions have not been well documented. Therefore, the aim of this study was to document PCIs encountered by post-menopausal osteoporotic women prescribed bisphosphonates. METHODS: A randomized controlled study was conducted from September 2005-February 2009 in the University Malaya Medical Centre, Malaysia. This main intervention study measured the effects of pharmaceutical care on medication adherence, persistence, quality of life, knowledge and patient satisfaction. However, this manuscript is part of the main intervention study and focuses only on …

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Physiological Responses Of Callus From Gerbera Jamesonii Bolus Ex. Hook F. To Gamma Irradiation, N.A. Hasbullah, R.M. Taha, A. Saleh, N. Mohamed Jan 2012

Physiological Responses Of Callus From Gerbera Jamesonii Bolus Ex. Hook F. To Gamma Irradiation, N.A. Hasbullah, R.M. Taha, A. Saleh, N. Mohamed

Research Publications (2011 to 2015)

In the present study, in vitro mutagenesis techniques were applied to investigate the effects of gamma irradiation at 0, 10, 20, 30, 40, 50 and 60 Gy on physiological changes in callus of Gerbera jamesonii Bolus ex. Hook f. Biochemical changes in chlorophyll and soluble protein content of pre- and post- irradiated Gerbera callus were studied. Non-irradiated callus demonstrated the highest amount of chlorophyll content as compared to callus irradiated at 10, 20, 30, 40, 50 and 60 Gy. In addition, the amount of chlorophyll b was relatively higher than chlorophyll a in both the irradiated and non-irradiated callus, except …

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