Digital Commons Network^™

Splendid Hybrids: The Effects Of A Tiger Beetle Hybrid Zone On Apparent Species Diversity, Mathew L. Brust, W. Wyatt Hoback, Stephen M. Spomer

Department of Entomology: Faculty Publications

Nonexpert citizen groups are being used to monitor species to track ecosystem changes; however, challenges remain for proper identification, especially among diverse groups such as beetles. Tiger beetles, Cicindela spp., have been used for biological diversity monitoring because of their diversity and the ease of recognition. The finding of an apparent hybrid zone among Cicindela denverensis Casey, Cicindela limbalis Klug, and Cicindela splendida Hentz in central Nebraska prompted a detailed study of the biogeography of this species group within Nebraska, a test of characteristics that could be used by citizen scientists, and limited breeding experiments. This study suggests that while …

Expected Economic Potential Of Substituting Legumes For Nitrogen In Bermudagrass Pastures, Jon T. Biermacher, The Samuel Roberts Noble Foundation, Inc. Reuter, M.K. Kering, J.K. Rogers, J. Blanton Jr, John A. Guretzky, T.J. Butler

Department of Agronomy and Horticulture: Faculty Publications

Grazing warm-season grass pastures with stocker cattle (Bos taurus) is an important economic activity in the southern Great Plains, and substantial increases in the price of N fertilizer have negatively affected profi tability of forage producers. The goal of the study was to determine if bermudagrass [Cynodon dactylon (L.) Pers.] pastures interseeded with either annual or perennial legumes are more profitable than the conventional method of fertilizing with 112 kg N ha^–1 commercial fertilizer. A completely randomized design grazing study was conducted in south-central Oklahoma during the spring and summer months of 2008, 2009, and 2010. …

Optimizing Cover Crop Benefits With Diverse Mixtures And An Alternative Termination Method, Samuel E. Wortman, Charles A. Francis, Mark L. Bernards, Rhae A. Drijber, John L. Lindquist

Department of Agronomy and Horticulture: Faculty Publications

Previous studies have demonstrated benefits of individual cover crop species, but the value of diverse cover crop mixtures has received less attention. The objectives of this research were to determine the effects of spring-sown cover crop mixture diversity and mechanical cover crop termination method on cover crop and/or cash crop productivity, soil moisture and N, and profitability in an organic cropping system. An experiment was conducted between 2009 and 2011 near Mead, Nebraska, United States, where mixtures of two (2CC), four (4CC), six (6CC), and eight (8CC) cover crop species, or a summer annual weed mixture were included in a …

Brown Midrib2 (Bmr2) Encodes The Major 4-Coumarate: Coenzyme A Ligase Involved In Lignin Biosynthesis In Sorghum (Sorghum Bicolor (L.) Moench), Ana Saballos, Scott Sattler, Emiliano Sanchez, Timothy P. Foster, Zhanguo Xin, Chulhee Kang, Jeffrey F. Pedersen, Wilfred Vermerris

Department of Agronomy and Horticulture: Faculty Publications

Successful modification of plant cell-wall composition without compromising plant integrity is dependent on being able to modify the expression of specific genes, but this can be very challenging when the target genes are members of multigene families. 4-coumarate:CoA ligase (4CL) catalyzes the formation of 4-coumaroyl CoA, a precursor of both flavonoids and monolignols, and is an attractive target for transgenic down-regulation aimed at improving agro-industrial properties. Inconsistent phenotypes of transgenic plants have been attributed to variable levels of down-regulation of multiple 4CL genes. Phylogenetic analysis of the sorghum genome revealed 24 4CL(-like) proteins, five of which cluster with bona fide …

Switchgrass Harvest And Storage, Robert B. Mitchell, Marty R. Schmer

Department of Agronomy and Horticulture: Faculty Publications

The feedstock characteristics of the conversion platform will influence the optimal harvest and post harvest management practices for switchgrass. However, many of the harvest management practices are tied to plant phenology and will be similar across platforms. Proper harvest and storage of switchgrass will help provide a consistent and high-quality feedstock to the biorefinery. Bioenergyspecific switchgrass strains are high-yielding and in most cases can be harvested and baled with commercially available haying equipment. Many options are available for packaging switchgrass for storage and transportation, but large round bales or large rectangular bales are the most readily available and are in …

Quantitative Trait Locus Analysis Of Saturated Fatty Acids In A Population Of Recombinant Inbred Lines Of Soybean, Xianzhi Wang, Guo-Liang Jiang, Marci Green, Roy A. Scott, D. L. Hyten, P. B. Cregan

Department of Agronomy and Horticulture: Faculty Publications

Soybean [Glycine max (L.) Merr.] is an important crop which contributes approximately 58% of the world’s oilseed production. Palmitic and stearic acids are the two main saturated fatty acids in soybean oil. Different levels of saturated fatty acids are desired depending on the uses of the soybean oil. Vegetable oil low in saturated fatty acids is preferred for human consumption, while for industrial applications, soybean oil with higher levels of saturated fatty acids is more suitable. The objectives of this study were to identify quantitative trait loci (QTL) for saturated fatty acids, analyze the genetic effects of single QTL …

Natural Variation In Iron Use Efficiency And Mineral Remobilization In Cucumber (Cucumis Sativus), Brian M. Waters, Grace Troupe

Department of Agronomy and Horticulture: Faculty Publications

Aims: Iron (Fe) is an essential micronutrient, and plant-available Fe is often limited in alkaline soils. Fe deficiency chlorosis decreases plant growth and yield. Identification of germplasm with high and low Fe use efficiency will allow studies to better understand the genetic components for breeding Fe efficient varieties.

Methods: A screen using cucumber (Cucumis sativus) seedlings identified varieties that maintained contrasting levels of chlorophyll under Fe deficiency or limitation. A time course of mineral dynamics in cotyledons was conducted.

Results: The variety Ashley had the highest chlorophyll under Fe deficiency and per unit Fe in the leaf, while …

Ascension, Megan Hopkins, David Thompson, Mary Beth Spence, Betsy Nolen, Katie Stambek, Paul Czech

Ascension: Elevating Research & Scholarship

No abstract provided.

Concerning Literacy Education For Students Enrolled In Kindergarten Through Third Grade, And, In Connection Therewith, Creating The "Colorado Early Literacy Act" And Making An Reducing Appropriations., Colorado General Assembly

Session Laws 2001-Present

No abstract provided.

Siv Infection Induces Accumulation Of Plasmacytoid Dendritic Cells In The Gut Mucosa, R. Keith Reeves, Tristan I. Evans, Jacqueline Gillis, Fay E. Wong, Guobin Kang, Qingsheng Li, R. Paul Johnson

Nebraska Center for Virology: Faculty Publications

Multiple studies suggest that plasmacytoid dendritic cells (pDCs) are depleted and dysfunctional during human immunodeficiency virus/simian immunodeficiency virus (HIV/SIV) infection, but little is known about pDCs in the gut—the primary site of virus replication. Here, we show that during SIV infection, pDCs were reduced 3-fold in the circulation and significantly upregulated the gut-homing marker α4β7, but were increased 4-fold in rectal biopsies of infected compared to naive macaques. These data revise the understanding of pDC immunobiology during SIV infection, indicating that pDCs are not necessarily depleted, but instead may traffic to and accumulate in the gut mucosa.

Triticum Mosaic Poacevirus Enlists P1 Rather Than Hc-Pro To Suppress Rna Silencing-Mediated Host Defense, Satyanarayana Tatineni, Feng Qu, Ruhui Li, Thomas Jack Morris, Roy French

Nebraska Center for Virology: Faculty Publications

Triticum mosaic virus (TriMV) is the type species of the newly established Poacevirus genus in the family Potyviridae. In this study, we demonstrate that in contrast to the helper component- proteinase (HC-Pro) of Potyvirus species, the P1 proteins of TriMV and Sugarcane steak mosaic poacevirus function in suppression of RNA silencing (SRS). TriMV P1 effectively suppressed silencing induced by single- or double-stranded RNAs (ss/ds RNAs), and disrupted the systemic spread of silencing signals at a step after silencing signal production. Interestingly, contrary to enhanced SRS activity of potyviral HC-Pro by co-expression with P1, the presence of TriMV HC-Pro reduced …

Toll-Like Receptor 7 Stimulates The Expression Of Epstein-Barr Virus Latent Membrane Protein 1, Robert M. Valente, Erica Ehlers, Dongsheng Xu, Humera Ahmad, Andrew Steadman, Laura Blasnitz, You Zhou, Lisa Kastanek, Bin Meng, Luwen Zhang

Nebraska Center for Virology: Faculty Publications

Epstein-Barr virus (EBV) is a ubiquitous human herpesvirus. Toll-like receptor 7 (TLR7) is involved in host innate immunity against pathogens, and its aberrant activation is linked to the development of systemic lupus erythematosus (SLE, also called ‘‘lupus’’). Type I interferons (IFN) are apparently driving forces for lupus pathogenesis. Previously, we found that EBV latent membrane protein 1 (LMP1) primes cells for IFN production. In this report, the relationship among EBV LMP1, TLRs, and IFN production are examined. We find that TLR7 activation increases the expression of EBV LMP1, and IFN regulatory factor 7 (IRF7) is involved in the stimulation process. …

Identification Of Molecular Determinants From Moloney Leukemia Virus 10 Homolog (Mov10) Protein For Virion Packaging And Anti-Hiv-1 Activity, Aierken Abudu, Xiaojun Wang, Ying Dang, Tao Zhou, Shi-Hua Xiang, Yong -Hui Zheng

Nebraska Center for Virology: Faculty Publications

Background: MOV₁₀ inhibits HIV-1 replication after being packaged.

Results: A Gag binding plus all but one of seven helicase domains are required for MOV^₁₀ packaging. Nearly all residues are required for anti-HIV-1 activity. Conclusion: Gag binding is not sufficient for MOV₁₀ packaging, and multiple discontinuous domains regulate MOV₁₀ activity.

Significance: These findings uncover a new packaging mechanism and provide new insights into MOV₁₀ antiviral activity.

Food Safety In Canada And The United States, Robert Strassfeld, Stephanie Lariviere, Cyndee Todgham Cherniak

Canada-United States Law Journal

No abstract provided.

2012 Niagara Problem And Bench Memo

Canada-United States Law Journal

No abstract provided.

Memorial Of The Respondent, Paul Allen, Kathleen D. Pitts

Canada-United States Law Journal

No abstract provided.

Fact Book 2011-2012, Various

General Fiscal Chronicle

Annual Report to the Board Of Trustees of George Fox College

‘Woman As…’: Personhood, Rights And The Case Of Domestic Violence, Stacy Missari, Christine Zozula

Societies Without Borders

This article uses the first domestic violence case filed against the United States in the Inter-American Commission on Human Rights (IACHR) to discuss the politics of gender and domestic violence. We discuss how gender-neutral frameworks of the case in the U.S. ignore the interpersonal gender and power issues which often attend domestic violence cases. The case before the IACHR was arguably more successful in addressing gender by drawing from the human rights literature on women’s rights. However, given that this case is the first human rights charge against the United States by a domestic violence survivor, the specifically gendered framework …

Nature, Society And Social Change, Michael Briguglio

Societies Without Borders

Environmental destruction has become an everyday reality in the contemporary world. Major concerns are being put forward regarding the dangers to the environment in general and to human societies in particular, with strong focus currently being put on climate change. Sociology has an important role to play in the analysis of environmental problems. The interaction between nature and society can be analysed through the concept of overdetermination. At the same time, the social construction on environmental problems is imperative for environmental issues to reach the agenda. An active environmental sociology which is as much concerned with analysis as it is …

Ctl Winter 2012 Newsletter, University Of Denver, Office Of Teaching And Learning

Office of Teaching and Learning Newsletters

Winter 2012 Newsletter from the University of Denver, Center for Teaching and Learning. The newsletter highlights events, workshops, software updates, and resources for faculty.

Similarity Between The Morphological Chromatophore Systems Present In The Autonomic Vertical Banding Display Of The Bluegill (Lepomis Macrochirus) And Selected Cichlids, Avery Thomson

Theses & ETDs

Certain fishes are unique in their ability to display skin color changes that reflect certain behavioral states. The vertical banding pattern (VB), the appearance of seven to nine dark vertical bands on the side of the fish, is a visual signal exhibited by certain percomorph fishes when they "feel" threatened. The bluegill centrarchid (Lepomis macrochirus) exhibits robust VB. Brain areas have been mapped for VB in centrarchids . The sympathetic nervous system is likely controlling the lightening/darkening of sets of chromatophores required for VB display. These cellular elements have been defined in cichlid fishes. This study visually confirms the similarity …

Spiritual Flow: The Influence Of Religious Experience On Psychological Well-Being, Christian H. Monson

All-Inclusive List of Electronic Theses and Dissertations

Researchers in the psychology of religion have attempted to better explain the relationship of religion/spiritual practice and psychological benefits, including the concept of well-being. The use of optimal experiences, or flow theory, has not yet been used in this endeavor. Flow is described as experiences which include meaningful enjoyment, focus, and intrinsic rewards and motivation. Since reports of transcendent, spiritual experiences appear similar to experiences of flow, it is likely that these experiences may overlap in structure. Also, since both religious and flow experiences have been associated with psychological well-being, it is likely that a spiritual flow experience may explain, …

Clinical Evaluation Of Novel Methods For Extending Microneedle Pore Lifetime, Nicole K. Brogden

Theses and Dissertations--Pharmacy

Microneedles are a minimally invasive method for delivering drugs through the impermeable skin layers, and have been used to deliver a variety of compounds including macromolecules, vaccines, and naltrexone. Microneedles can be applied to the skin once, creating micropores that allow for drug delivery into the underlying circulation from a drug formulation. The utility of this technique, however, is blunted by rapid micropore closure. This research project sought to: 1) characterize micropore lifetime and re-sealing kinetics, and 2) prolong micropore lifetime via inhibition of the skin’s barrier restoration processes. Impedance spectroscopy was used as a surrogate technique in animals and …

An Ims/Atp Assay For The Detection Of Mycobacterium Tuberculosis In Urine, Dawn M. Hunter, Daniel V. Lim

Molecular Biosciences Faculty Publications

Background. Although sputum smears are the gold standard for diagnosis of tuberculosis, sensitivity in HIV/TB coinfection cases is low, indicating a need for alternative methods. Urine is being increasingly evaluated. Materials and Methods. A novel method for detecting Mycobacterium tuberculosis (MTB) in synthetic urine using a combined IMS/ATP assay was evaluated. Preliminary work established standard ATP conditions and the sensitivity and specificity of the MTB antibody. Eighty-four blinded samples in four replicate assays were evaluated for the presence of MTB using labeled immunomagnetic beads for capture. Beads were separated, washed, and resuspended in broth and added to a …

Identification Of New Cell Size Control Genes In S. Cerevisiae, Huzefa Dungrawala, Hui Hua, Jill Wright, Lesley Abraham, Thivakorn Kasemsri, Anthony Mcdowell, Jessica Stilwell, Brandt L. Schneider

Molecular Biosciences Faculty Publications

Cell size homeostasis is a conserved attribute in many eukaryotic species involving a tight regulation between the processes of growth and proliferation. In budding yeast S. cerevisiae, growth to a “critical cell size” must be achieved before a cell can progress past START and commit to cell division. Numerous studies have shown that progression past START is actively regulated by cell size control genes, many of which have implications in cell cycle control and cancer. Two initial screens identified genes that strongly modulate cell size in yeast. Since a second generation yeast gene knockout collection has been generated, we screened …

The Two-Component Signal Transduction System Arlrs Regulates Staphylococcus Epidermidis Biofilm Formation In An Ica-Dependent Manner, Yang Wu, Jiaxue Wang, Tao Xu, Jingran Liu, Wenqi Yu, Qiang Lou, Tao Zhu, Nianan He, Haijing Ben, Jian Hu, Friedrich Götz, Di Qu

Molecular Biosciences Faculty Publications

Due to its ability to form biofilms on medical devices, Staphylococcus epidermidis has emerged as a major pathogen of nosocomial infections. In this study, we investigated the role of the two-component signal transduction system ArlRS in regulating S. epidermidis biofilm formation. An ArlRS-deficient mutant, WW06, was constructed using S. epidermidis strain 1457 as a parental strain. Although the growth curve of WW06 was similar to that of SE1457, the mutant strain was unable to form biofilms in vitro. In a rabbit subcutaneous infection model, sterile disks made of polymeric materials were implanted subcutaneously followed with inoculation of WW06 or SE1457. …

Wipi-1 Positive Autophagosome-Like Vesicles Entrap Pathogenic Staphylococcus Aureus For Lysosomal Degradation, Mario Mauthe, Wenqi Yu, Oleg Krut, Martin Krönke, Friedrich Götz, Horst Robenek, Tassula Proikas-Cezanne

Molecular Biosciences Faculty Publications

Invading pathogens provoke the autophagic machinery and, in a process termed xenophagy, the host cell survives because autophagy is employed as a safeguard for pathogens that escaped phagosomes. However, some pathogens can manipulate the autophagic pathway and replicate within the niche of generated autophagosome-like vesicles. By automated fluorescence-based high content analyses, we demonstrate that Staphylococcus aureus strains (USA300, HG001, SA113) stimulate autophagy and become entrapped in intracellular PtdIns(3)P-enriched vesicles that are decorated with human WIPI-1, an essential PtdIns(3)P effector of canonical autophagy and membrane protein of both phagophores and autophagosomes. Further, agr-positive S. aureus (USA300, HG001) strains were more efficiently …

Research Week 2012, Linda Gardiner

Office of Research Institutional Research and Scholarship

No abstract provided.

Saintly Bodies, Mortal Bodies: Hagiographic Decoration On English Twelfth Century Baptismal Fonts, Frances Altvater

Peregrinations: Journal of Medieval Art and Architecture

No abstract provided.

Primary Uterine Cervix Schwannoma: A Case Report And Review Of The Literature, Maryam Tahmasbi, Johnny Nguyen, Masoumeh Ghayouri, Yuan Shan, Ardeshir Hakam

Pathology and Cell Biology Faculty Publications

Schwannoma (neurilemmoma) is a benign peripheral nerve sheath tumor that occurs in a wide variety of locations; however, its finding in the uterine cervix is extremely rare. We report a case of an incidental primary benign cervical schwannoma in a 48-year-old woman. In the English literature, a few cases of primary schwannoma of the cervix have been reported, which include seven cases of primary malignant cervical schwannoma and only two that are benign. These cases are reviewed in the following discussion.