Digital Commons Network^™

Chapter 4: Crop Management Of Switchgrass, Matt A. Sanderson, Marty Schmer, Vance Owens, Pat Keyser, Wolter Elbersen

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Management of switchgrass for bioenergy and forage share some commonalities, of particular interest in bioenergy crop production is: (1) rapid establishment of switchgrass to generate harvestable biomass in the seeding year, (2) highly efficient management of soil and fertilizer N to minimize external energy inputs, and (3) harvest management to maximize yields of lignocellulose. Bioenergy cropping may entail management for multiple services in addition to biomass yield including soil C sequestration, wildlife habitat, landscape management, and water quality protection. Management is a critical factor especially as land classified as marginal or idle land will be emphasized for bioenergy production to …

Efficacy Of Proximity Loggers For Detection Of Contacts Between Maternal Pairs Of White-Tailed Deer, Ryan Walrath, Timonthy R. Vandeelen, Kurt C. Vercauteren

United States Department of Agriculture Wildlife Services: Staff Publications

Contact frequency and duration estimates between individuals are important to understanding the behavioral ecology of wildlife species and the epidemiology of infectious diseases. A new technology uses proximity data loggers to record time and duration of contacts. We conducted an experiment at Sandhill Wildlife Management Area, located near Babcock, Wisconsin (USA) to compare probabilities of detecting intraspecific contacts among white-tailed deer (Odocoileus virginianus) maternal pairs (dams/fawns) based on detections from proximity loggers deployed on collars versus those obtained from direct observation. We defined 5 discrete probabilities of detection of a contact in terms of P (probability of detection …

Safety And Immunogenicity Of Onrab® In Raccoons And Skunks In West Virginia – 2011 Field Trial Report, D. D. Slate, R. Chipman, K. Nelson, C. Croson, S. Mills, C. Rupprecht, K. C. Vercauteren

United States Department of Agriculture Wildlife Services: Staff Publications

In 2011, a field trial was conducted in southeastern West Virginia to evaluate the safety and immunogenicity of a live recombinant human adenovirus (serotype 5)-rabies glycoprotein vaccine (ONRAB®) in wild raccoons and skunks. ONRAB® was selected for evaluation based on the success achieved in eliminating raccoon rabies from Ontario and Quebec, Canada, through the integration of this vaccine into rabies management strategies and significantly higher sero-positivity in comparison to Raboral V-RG® in studies along the U.S.-Canada border. The field trial site was composed of forested and agricultural habitats in an area where raccoon rabies is enzootic and oral rabies vaccine …

Invasives: The High Cost Of Indifference, Mark Pfost, Kurt C. Vercauteren

United States Department of Agriculture Wildlife Services: Staff Publications

At one time, wildlife professionals may have been blissfully ignorant about invasive species, classifying “plants” as lawn grass, flowers, trees, or weeds, and putting animals into two groups: species with bag limits and everything else. But those days of ignorance are long past. We see how invasives threaten local, even global, ecosystems, and we need to help create greater awareness about the scope of the problem.

Wildlife professionals must not only prepare to deal with how seven billion people, climate change, and habitat loss will affect native species and habitats, but also how those species and habitats will be affected …

Fitting Statistical Distributions To Sea Duck Count Data: Implications For Survey Design And Abundance Estimation, Elise F. Zipkin, Jeffery B. Leirness, Brian P. Kinlan, Allan F. O’Connell, Emily D. Silverman

United States Geological Survey: Staff Publications

Determining appropriate statistical distributions for modeling animal count data is important for accurate estimation of abundance, distribution, and trends. In the case of sea ducks along the U.S. Atlantic coast, managers want to estimate local and regional abundance to detect and track population declines, to define areas of high and low use, and to predict the impact of future habitat change on populations. In this paper, we used a modified marked point process to model survey data that recorded flock sizes of Common eiders, Long-tailed ducks, and Black, Surf, and White-winged scoters. The data come from an experimental aerial survey, …

History And Evaluation Of National-Scale Geochemical Data Sets For The United States, David B. Smith, Steven M. Smith, John D. Horton

United States Geological Survey: Staff Publications

Six national-scale, or near national-scale, geochemical data sets for soils or stream sediments exist for the United States. The earliest of these, here termed the ‘Shacklette’ data set, was generated by a U.S. Geological Survey (USGS) project conducted from 1961 to 1975. This project used soil collected from a depth of about 20 cm as the sampling medium at 1323 sites throughout the conterminous U.S. The National Uranium Resource Evaluation Hydrogeochemical and Stream Sediment Reconnaissance (NUREHSSR) Program of the U.S. Department of Energy was conducted from 1975 to 1984 and collected either stream sediments, lake sediments, or soils at more …

Hepatic Abca1 And Vldl Triglyceride Production, Mingxia Liu, Soonkyu Chung, Gregory S. Shelness, John S. Parks

Department of Nutrition and Health Sciences: Faculty Publications

Elevated plasma triglyceride (TG) and reduced high density lipoprotein (HDL) concentrations are prominent features of metabolic syndrome (MS) and type 2 diabetes (T2D). Individuals with Tangier disease also have elevated plasma TG concentrations and a near absence of HDL, resulting from mutations in ATP binding cassette transporter A1 (ABCA1), which facilitates the efflux of cellular phospholipid and free cholesterol to assemble with apolipoprotein A-I (apoA-I), forming nascent HDL particles. In this review, we summarize studies focused on the regulation of hepatic very low density lipoprotein (VLDL) TG production, with particular attention on recent evidence connecting hepatic ABCA1 expression to VLDL, …

Hepatic Abc Transporters And Triglyceride Metabolism, John S. Parks, Soonkyu Chung, Gregory S. Shelness

Department of Nutrition and Health Sciences: Faculty Publications

Purpose of review—Elevated plasma triglyceride and reduced HDL concentrations are prominent features of metabolic syndrome and type 2 diabetes. Individuals with Tangier disease also have elevated plasma triglyceride concentrations and very low HDL, resulting from mutations in ATP-binding cassette transporter A1 (ABCA1), an integral membrane protein that facilitates nascent HDL particle assembly. Past studies attributed the inverse relationship between plasma HDL and triglyceride to intravascular lipid exchange and catabolic events. However, recent studies also suggest that hepatic signaling and lipid mobilization and secretion may explain how HDL affects plasma triglyceride concentrations.

Recent findings—Hepatocyte-specific ABCA1 knockout mice have markedly reduced plasma …

Regulation Of Expression Of Citrate Synthase By The Retinoic Acid Receptor-Related Orphan Receptor A (Rorα), Christine Crumbley, Yongjun Wang, Subhashis Banerjee, Thomas P. Burris

Department of Nutrition and Health Sciences: Faculty Publications

The retinoic acid receptor-related orphan receptor α (RORα) is a member of the nuclear receptor superfamily of transcription factors that plays an important role in regulation of the circadian rhythm and metabolism. Mice lacking a functional RORα display a range of metabolic abnormalities including decreased serum cholesterol and plasma triglycerides. Citrate synthase (CS) is a key enzyme of the citric acid cycle that provides energy for cellular function. Additionally, CS plays a critical role in providing citrate derived acetyl-CoA for lipogenesis and cholesterologenesis. Here, we identified a functional RORα response element (RORE) in the promoter of the CS gene. ChIP …

On Thinning Of Chains In Mcmc, William A. Link, Mitchell J. Eaton

United States Geological Survey: Staff Publications

1. Markov chain Monte Carlo (MCMC) is a simulation technique that has revolutionised the analysis of ecological data, allowing the fitting of complex models in a Bayesian framework. Since 2001, there have been nearly 200 papers using MCMC in publications of the Ecological Society of America and the British Ecological Society, including more than 75 in the journal Ecology and 35 in the Journal of Applied Ecology.

2. We have noted that many authors routinely ‘thin’ their simulations, discarding all but every kth sampled value; of the studies we surveyed with details on MCMC implementation, 40% reported thinning.

3. Thinning …

Spatially Explicit Models For Inference About Density In Unmarked Or Partially Marked Populations, Richard B. Chandler, J. Andrew Royle

United States Geological Survey: Staff Publications

Recently developed spatial capture-recapture (SCR) models represent a major advance over traditional capture-recapture (CR) models because they yield explicit estimates of animal density instead of population size within an unknown area. Futhermore, unlike nonspatial CR methods, SCR models account for heterogeneity in capture probability arising from the juxtaposition of animal activity centers and sample locations. Although the utility of SCR methods is gaining recognition, the requirement that all individuals can be uniquely identified exludes their use in many contexts. In this paper, we develop models for situations in which individual recognition is not possible, thereby allowing SCR concepts to be …

On Thinning Of Chains In Mcmc, William A. Link, Mitchell J. Eaton

United States Geological Survey: Staff Publications

1. Markov chain Monte Carlo (MCMC) is a simulation technique that has revolutionised the analysis of ecological data, allowing the fitting of complex models in a Bayesian framework. Since 2001, there have been nearly 200 papers using MCMC in publications of the Ecological Society of America and the British Ecological Society, including more than 75 in the journal Ecology and 35 in the Journal of Applied Ecology.

2. We have noted that many authors routinely ‘thin’ their simulations, discarding all but every kth sampled value; of the studies we surveyed with details on MCMC implementation, 40% reported thinning.

3. Thinning …

Anticholinergic Impact On Cognition In Schizophrenia, Catherine Underwood

Yale School of Nursing Digital Theses

This secondary analysis examined the relationship between the total anticholinergic load and scores on cognitive tests in 94 patients diagnosed with schizophrenia or schizoaffective disorder. Cognitive impairment is a core feature of schizophrenia, and studies have shown that it is a strong predictor of functional outcomes in this population. Anticholinergic medications have been demonstrated to have a negative impact on cognition. Some antipsychotic medications have anticholinergic properties, and anticholinergic medications are often prescribed to patients with schizophrenia to treat or prevent extrapyramidal side effects of antipsychotics. We did not find any significant correlations between anticholinergic load as measured by benztropine …

Emerging Microbial And Viral Infections Of The Central Nervous System, Brian J. Balin, Christine J. Hammond

PCOM Scholarly Works

This review focuses on emerging viral and bacterial infections in the human central nervous system (CNS) that are responsible for significant global morbidity and mortality. These infections include those responsible for acute neurological disease such as meningitis and encephalitis as well those associated with chronic neurodegenerative conditions. Recent changes in climate conditions and pollution have been precipitating factors leading to the emergence of many of these pathogenic organisms. In addition, increased urbanization, global travel, life span, and exposure to new vectors have promoted the organisms’ spread across the globe. Categorization of many of these organisms includes identification of new species, …

All Pain, No Gain: Need For Prudent Antimicrobial Use Provisions To Complement The Gain Act, Kevin Outterson

Faculty Scholarship

Once every five years, Congress re-authorizes funding for the FDA under PDUFA. In the current PDUFA legislation, Congress also addressed issues relating to antimicrobial resistance in the Generating Antibiotic Incentives Now (GAIN) Act. This short article critiques the absence of antimicrobial stewardship incentives in the GAIN Act, which is a serious error in the long-term struggle with pathogenic microbes.

Green Transportation For A Green Earth, Petros J. Katsioloudis, Mildred V. Jones

STEMPS Faculty Publications

Transportation is one of the biggest contributors to the destruction of the environment. Results from environmental research and pursuant governmental regulations are changing the way companies do business and how transportation systems address the future. The Environmental Protection Agency (EPA) has been at the forefront in leading environmental research and instituting policies and regulations to fight the issues impacting the environment. The transportation industry and consumers alike are concerned about the environment, energy resources, efficiencies, and economics. In this article, the authors suggest that it is vital that people promote the protection of the environment and strengthen the laws that …

Stacking Of Dangerous Goods In Accordance With United Nations Stacking Recommendation, Zachary Loughery

Theses

The purpose of the study was to determine if the possible interpretations of the UN stacking recommendation could lead to different results. If this is possible the recommendation would need to be rewritten to eliminate the possibility of the same product passing or failing within the standard. The focus of this study was a plastic jerrican filled with water. The acceptable methods that are mentioned in the recommendation are the type of compression and the optional use of a fixture to simulate nesting. Each jerrican was filled in accordance with the recommendation and allowed to Condition in the testing room …

No History, No Certainty, No Legitimacy . . . No Problem: Originalism And The Limits Of Legal Theory, Gary S. Lawson

Faculty Scholarship

Professor Martin H. Redish is on the warpath. Like General Sherman marching toward Atlanta (or Justin Tuck marching toward Tom Brady), Professor Redish, together with Matthew Arnould,1 lays waste to every constitutional theory that he encounters. Originalism, with its “belief that constitutional interpretation should be characterized exclusively by an effort to determine the Constitution’s meaning by means of some form of historical inquiry,”2 generates “an often contrived and opaque veil of historical inquiry”3 that provides “an ideal smokescreen behind which judges may pursue their personal[,] moral, political[,] or economic goals with relative impunity.”4 Nontextual theories, for their part, “permit[] selective …

Dominant Eukaryotic Export Production During Ocean Anoxic Events Reflects The Importance Of Recycled Nh4+, Meytal B. Higgins, Rebecca S. Robinson, Jonathan M. Husson, Susan J. Carter, Ann Pearson

Graduate School of Oceanography Faculty Publications

The Mesozoic is marked by several widespread occurrences of intense organic matter burial. Sediments from the largest of these events, the Cenomanian–Turonian Oceanic Anoxic Event (OAE 2) are characterized by lower nitrogen isotope ratios than are seen in modern marine settings. It has remained a challenge to describe a nitrogen cycle that could achieve such isotopic depletion. Here we use nitrogen-isotope ratios of porphyrins to show that eukaryotes contributed the quantitative majority of export production throughout OAE 2, whereas cyanobacteria contributed on average approximately 20%. Such data require that any explanation for the OAE nitrogen cycle and its isotopic values …

Vibrio Parahaemolyticus In Rhode Island Coastal Ponds And The Estuarine Environment Of Narragansett Bay, Annie M. Cox, Marta Gomez-Chiarri

Graduate School of Oceanography Faculty Publications

Quantification of the abundance of Vibrio parahaemolyticus in water and oysters from Rhode Island showed the presence of environmental strains and low levels of potentially pathogenic strains when water temperatures were ≥18°C, with peak levels in late July to early August. A higher abundance of the trh gene than of the tdh gene was observed.

Cell Cycle Arrest Associated With Anoxia-Induced Quiescence, Anoxic Preconditioning, And Embryonic Diapause In Embryos Of The Annual Killifish Austrofundulus Limnaeus, Camie Lynn Meller, Robert Meller, Roger P. Simon, Kristin M. Culpepper, Jason E. Podrabsky

Biology Faculty Publications and Presentations

Embryos of the annual killifish Austrofundulus limnaeus can enter into dormancy associated with diapause and anoxia-induced quiescence. Dormant embryos are composed primarily of cells arrested in the G1/G0 phase of the cell cycle based on flow cytometry analysis of DNA content. In fact, most cells in developing embryos contain only a diploid complement of DNA, with very few cells found in the S, G2, or M phases of the cell cycle. Diapause II embryos appear to be in a G0-like state with low levels of cyclin D1 and p53. However, the active form of pAKT is high during diapause II. …

Anoxia Tolerance During Vertebrate Development - Insights From Studies On The Annual Killifish Austrofundulus Limnaeus, Jason E. Podrabsky, Claire L. Riggs, Jeffrey M. Duerr

Biology Faculty Publications and Presentations

This is chapter 1 from Anoxia -- This book reviews how severe oxygen deprivation affects biological systems - from the molecular to the ecological level. The contributing authors come from diverse regions of the world, which proves the interest in the academic analysis of oxygen deprivation. The diversity in the experimental approach scientists take, in order to understand the influence oxygen deprivation has on living systems, is apparent throughout this book. One of the presented ideas deals with the exploration and examination of the physiological, cellular and genetic characteristics of killifish embryos and nematodes exposed to anoxia. Furthermore, the book …

An Epidemiological Model Of Rift Valley Fever With Spatial Dynamics, Tianchan Niu, Holly D. Gaff, Yiannis E. Papelis, David M. Hartley

Biological Sciences Faculty Publications

As a category A agent in the Center for Disease Control bioterrorism list, Rift Valley fever (RVF) is considered a major threat to the United States (USA). Should the pathogen be intentionally or unintentionally introduced to the continental USA, there is tremendous potential for economic damages due to loss of livestock, trade restrictions, and subsequent food supply chain disruptions. We have incorporated the effects of space into a mathematical model of RVF in order to study the dynamics of the pathogen spread as affected by the movement of humans, livestock, and mosquitoes. The model accounts for the horizontal transmission of …

Characterizing Dissolved Organic Matter And Cu2+, Zn2+, Ni2+, And Pb2+ Binding In Salt Water And Implications For Toxicity, Rachael L. Diamond

Theses and Dissertations (Comprehensive)

The binding of metal to dissolved organic matter in aquatic environments is important in controlling the bioavailability and potential toxicity of metals such as Zn²⁺, Pb²⁺, Ni²⁺ and Cu²⁺. The purpose of this research is to: (i) quantify binding capacity to different sources of marine organic matter at environmentally relevant concentrations; (ii) test fluorescence quenching and voltammetric method for use in seawater conditions and; (iii) compare predicted speciation parameters with toxicological observations in the same samples.

Information regarding the solubility of copper and copper compounds is important for risk assessment and can be …

Proteomic Analysis Of The Highly Detergent Resistant Radial Component Of Myelin, Vatsal M. Patel

Theses and Dissertations (Comprehensive)

The wrapping of multiple layers of myelin membrane sheets around an axon plays an important role in normal neuronal function. In the central nervous system (CNS), oligodendrocytes are responsible for forming multiple myelin internodes which consist of lipids and proteins. Within these regions, there are various membrane microdomains including the radial component/tight junctions. The radial component is a junctional complex believed to stabilize the myelin membranes in the CNS myelin. In this study, the radial component from bovine and mouse myelin was isolated using differential detergent extraction. A highly detergent resistant fraction, containing previously identified proteins of the radial component …

Atr-Ftir Studies On The Kinetics Of Dimethylarsinic Acid (Dma) Surface Complexation With Iron(Oxyhydr)Oxides, Julia Tofan-Lazar Miss

Theses and Dissertations (Comprehensive)

Dimethylarsinic acid (DMA) is an organoarsenical compound that, along with monomethylarsonic acid, poses a health and an environmental risk, and a challenge to the energy industry. Little is known about the surface chemistry of DMA at the molecular level with materials relevant to geochemical environments and industrial sectors. The surface chemistry of phosphorus and arsenic compounds in their organic and inorganic forms is of great interest due to its role in controlling their transport, bioaccessibility and speciation. This thesis reports the first in-situ and surface-sensitive rapid kinetic studies on the adsorption and desorption of DMA to and from Fe-(oxyhydr)oxide films …

In Vitro Propagation Of Candidatus Rickettsia Andeanae Isolated From Amblyomma Maculatum, Alison Luce-Fedrow, Chelsea Wright, Holly D. Gaff, Daniel E. Sonenshine, Wayne L. Hynes, Allen L. Richards

Biological Sciences Faculty Publications

Candidatus Rickettsia andeanae was identified during an investigation of a febrile outbreak in northwestern Peru (2002). DNA sequencing from two ticks (Amblyomma maculatum, Ixodes boliviensis) collected during the investigation revealed a novel Rickettsia agent with similarity to the spotted fever group rickettsiae. Since then, Candidatus R. similar to andeanae has been detected in A. maculatum ticks collected in the southeastern and southcentral United States, Argentina, and Peru. To date, Candidatus R. andeanae has not been successfully cultivated in the laboratory. We present evidence for the continuous cultivation in three cell lines of Candidatus R. similar to …

Protandric Simultaneous Hermaphroditism In Parhippolyte Misticia (Clark, 1989) (Caridea: Hippolytidae): Implications For The Evolution Of Mixed Sexual Systems In Shrimp, Hitoshi Onaga, G. Curt Fiedler, J. Antonio Baeza

Biological Sciences Faculty Publications

The sexual system of the shrimp Parhippolyte misticia (Clark, 1989), inhabiting the rocky subtidal at Okinawa, Japan and Kimbe Bay, Papua New Guinea, was examined. Dissections suggested that the population consisted of male phase (MP) and functional simultaneous euhermaphrodite (EH) individuals. MPs have cincinulli and appendices masculinae on the first and second pair of pleopods, respectively, gonopores located at the coxae of the third pair of walking legs, and ovotestes with a well-developed male portion containing sperm, but an undeveloped female portion. EHs lacked appendices masculinae and cincinulli. However, they have male gonopores and ovotestes with well-developed ovaries containing mature …

The Effect Of Cadmium Exposure On Repeat Swimming Performance And Recovery In Rainbow Trout (Oncorhynchus Mykiss), Brown Trout (Salmo Trutta) And Lake Whitefish (Coregonus Clupeaformis), Jessie L. Cunningham

Theses and Dissertations (Comprehensive)

Swim performance provides a measure of the integrated metabolic costs of sub-lethal exposure to stressors, including Cd. While the impacts to sustained swimming performance in Cd exposed fish have already been characterised, effects to repeat swimming ability have not. This thesis identifies the effects of sub-lethal waterborne Cd exposure on ionoregulatory function, repeat swimming ability, anaerobic metabolism and stress response in rainbow trout (Oncorhynchus mykiss), brown trout (Salmo trutta) and lake whitefish (Coregonus clupeaformis).

Repeat swim trials included an initial swim to 85% of the U_crit of control fish and a recovery period …

The Effects Of 17Α-Ethynylestradiol (Ee2) On Gonadal Development And Differentiation In The Estuarine Killifish, Fundulus Heteroclitus, Ibrahim Chehade

Theses and Dissertations (Comprehensive)

Endocrine disrupting substances (EDSs) comprise a wide variety of chemicals that perturb normal endocrine function including developmental and reproductive processes in vertebrates. The synthetic estrogen 17α-ethynylestradiol (EE₂) is a commonly-used model EDS because of its environmental relevance and its effects on the reproductive endocrine system. Early life-stage exposure of fish to estrogenic EDSs causes effects such as intersex (ovotestes in males) and feminization. This study aims to confirm the period of gonadal differentiation in the estuarine killifish or mummichog (Fundulus heteroclitus) and to determine the sensitivity of gonadal development to EE₂. Artificially-regressed mummichog were spawned, and …