Digital Commons Network^™

Sex Differences In The Cardiac Beta-Adrenergic Receptor Contractile Response, Victoria Mcintosh

Wayne State University Dissertations

Premenopausal females are at a reduced risk for developing cardiovascular disease as compared to males; this sex difference is not present following menopause. &beta-adrenergic receptors are pharmaceutical targets in the treatment of cardiovascular disease, and sex differences in &beta-adrenergic responsiveness have been demonstrated. However, limited studies have addressed the mechanism(s) underlying these differences. To investigate these sex differences, studies were performed using isolated perfused hearts from male, intact female and ovariectomized female mice, as well as male and female ventricular myocytes. Female hearts exhibited blunted contractile responses to the &beta-adrenergic receptor agonist isoproterenol (ISO) compared to males but not ovariectomized …

Hallowed Ground: Literature And The Encounter With God In Post-Reformtion England, C. 1550 - 1704, Michael Thomas Martin

Wayne State University Dissertations

This dissertation examines the ways in which the encounter with God is figured in post-Reformation English writing between the years 1550 and 1704. The introduction contextualizes the ways in which individuals might encounter God within cultural and historical circumstances of the period: the gradual disappearance of the tradition of spiritual direction that accompanied the suppression of Catholicism in England during the period and the growing influence of more purely "scientific" modes of inquiry, especially after Descartes. Because of these changes, the ways the encounter with God could be experienced were also changing. The introduction also shows how developments in religious …

Motion(Less) Pictures: The Cinema Of Stasis, Justin Remeselnik

Wayne State University Dissertations

Since cinema's inception, there has been much disagreement among film theorists about the role of movement in cinema's ontology. For example, while Rudolf Arnheim has argued that motion is a sine qua non of cinema, Roland Barthes has insisted that motion is not as central to cinema's ontology as duration, an experiential "unfolding." In this dissertation, I argue--following Barthes--that movement is merely a contingent, not a necessary, condition of cinema. I further suggest that the very enterprise of prescribing necessary conditions of cinema is myopic, reductive, and reactionary.

In supporting these claims, I interrogate the cinema of stasis, a modality …

Identifying Sm22 As A Key Player In Arterial Diseases, Jianbin Shen

Wayne State University Dissertations

Background : Expression of vascular smooth muscle cell (VSMC) cytoskeleton markers including SM22 is down-regulated in arterial diseases including atherosclerosis where inflammation and osteochondrogenesis are present. However, the role of this downregulation in arterial pathogenesis is unknown. Hypothesis : Downregulation of SM22 may actively contribute to arterial pathogenesis. Methods : Five Sm22 knockout (Sm22^-/-) mice and their wild type littermates were subjected to carotid artery denudation, an artery injury model. Analyses were conducted on carotid arteries 2 weeks after injury. Primary VSMCs were isolated from mouse aortas and investigated individually at passage 2 to 4. Sm22 knockdown was …

Determinants Of Cns Adult Neurogenesis Are Sex, Hormones, Mouse Strain, Age, And Brain Region, Carrie Lynn Tatar

Wayne State University Dissertations

Multiple sclerosis is a sexually dimorphic (SD) disease that causes oligodendrocyte death but SD of glial cells is poorly studied. Here, we analyze SD of neural progenitors in 6-8wk and 6-8mo normal C57BL/6, SJL/J and BALB/c mice in the subventricular zone (SVZ), dorsolateral horn (DLC), corpus callosum (CC), and parenchyma. With a short 2 h BrdU pulse, no gender and strain differences are present at 6-8wk. At 6-8mo, the number of BrdU+ cells decreases 2-fold in both sexes, strains and regions indicating a common aging mechanism regulates BrdU incorporation. Strikingly, 2X more BrdU+ cells are found in all brain regions …

Investigation Of The Absorptive Potential Of Polymeric Nanoparticles Across The Lungs And Their Development For Efficient Systemic Delivery Following Pulmonary Administration, Abdul Khader Mohammad

Wayne State University Dissertations

The lungs are an attractive route for drug delivery due to their high epithelial surface area available for absorption, a thin epithelium and extensive vasculature to name a few. Accordingly, a vast number of small molecule drugs, peptides, and proteins have been used to investigate their translocation across the lungs with many of the tested candidates showing excellent pharmacokinetics following pulmonary administration. Findings from all these studies over the years have strongly established the lungs as a route for drug delivery of small molecules and proteins. Nanoparticles on the other hand have gained increasing interest in drug delivery due to …

Wired & Dangerous: Hacks, Hair Extensions And Other Twists On Traditional Technical Communication, Hilary Anne Sarat-St Peter

Wayne State University Dissertations

Ever since its inception as a "humanistic" research discipline (Miller, 1979; Dombrowski, 1994), technical communication has striven to balance workplace exigencies with attention to the broader rhetorical, social and ethical issues within which technical communication is situated. Recently, this humanistic agenda has expanded from a simple awareness of contextual factors surrounding work (see, for example, Collier and Toomey, 1997) to calls for technical communication research in non-workplace and other non-traditional sites. Frequently these calls for "extra-institutional" research (Kimball, 2007) are driven by the assumption that users' indigenous technical communication is inherently more user-centered - and therefore more democratic - than …

Identification Of Transcriptional Mechanisms Downstream Of Nf1 Gene Defeciency In Malignant Peripheral Nerve Sheath Tumors, Daochun Sun

Wayne State University Dissertations

Malignant peripheral nerve sheath tumor (MPNST) is a type of soft tissue sarcoma that occurs in carriers of mutations in the neurofibromatosis type I gene (Nf1) as well as sporadically. Plexiform neurofibromas in NF1 patients have a significant risk of developing into MPNSTs leading to increased morbidity and mortality from this syndrome. Surgery is the primary intervention but it is not always effective due to the tendency of MPNSTs to infiltrate the surrounding tissue or grow in an inoperable location. Neurofibromin, the protein coded by the Nf1 gene, functions as a GTPase activating protein (GAP) whose mutation leads to constitutive …

Understanding The Gender-Based Mechanism Of Mso In Als Mice: A Metabolic Characterization Of The Sod1-G93a Mouse Model, Monica Ann Bame

Wayne State University Dissertations

Amyotrophic Lateral Sclerosis (ALS) is a devastating neurodegenerative disease characterized by motor neuron death and a corresponding loss of neuromuscular connections resulting in muscle atrophy. Patients become paralyzed shortly after symptom onset and typically die within one to five years of pulmonary complications. ALS is a relatively rare disease, with an overall incidence of approximately 2 in 100,000 people per year and a prevalence of about 5 in 100,000 people. It is typically associated with increasing age and has a slight male prevalence, with a male to female ratio of approximately 3:2. ALS is classified as either familial (the less …

Examining Autoimmune Sequelae During Cancer Immunotherapy In A Combined Autoimmune Thyroiditis And Tumor Mouse Model, Suresh Kari

Wayne State University Dissertations

Some clinical trials of immunotherapeutic agents against cancers have resulted in the development of autoimmune sequelae, including autoimmune thyroiditis. It has been suggested that the immunotherapy resulted in an alteration in the immunoregulatory mechanism(s). Using experimental autoimmune thyroiditis (EAT), a mouse model of Hashimoto's thyroiditis (HT), the importance of regulatory T cells (Tregs) has been well established; inhibition of Tregs leads to enhanced thyroiditis with repeated doses of mouse thyroglobulin (mTg) without adjuvant. To simulate cancer patients, we developed a combined EAT and tumor model in our EAT-susceptible mice (CBA/J). In first establishing the tumor model, we found Treg depletion …

Multifunctional Bioreducible Nanoparticles For Gene Therapy, Jing Li

Wayne State University Dissertations

Gene therapy is a promising therapeutic strategy to treat diseases caused by single or multiple gene mutations. Non-viral gene delivery vectors exhibit many advantages over viral vectors, including enhanced safety, versatility in choosing different types of therapeutic nucleic acids, and ease of formulation. However, low transgene efficiency and lack of targeting ability remain major challenges. Bioreducible polyplexes (polyelectrolyte complexes of disulfide-containing polycations and nucleic acids) utilize the redox potential gradient existing between extracellular space and intracellular environment as a physiological stimulus to enhance delivery of therapeutic nucleic acids to the subcellular space. Bioreducible containing polyplexes undergo intracellular reduction mediated GSH, …

A Multi-Agent Intelligent System For Detecting Unknown Adverse Drug Reactions Through Communication And Collaboration, Ayman Mohammad Mansour

Wayne State University Dissertations

Several thousands of drugs are currently available on the U.S. market. A complete understanding of the safe use of drugs is not possible at the time when drug is developed or marketed. At that time, the safety information is only obtained from a few thousand people in a typical pre-marketing clinical trial. Clinical trials are not capable of detecting rare adverse drug reactions (ADRs) because of limitations in sample size and trial duration. Early detection of unknown ADRs could save lives and prevent unnecessary hospitalizations. Current methods largely rely on spontaneous reports which suffer from serious underreporting, latency, and inconsistent …

Role Of Thromboxane Receptor-Alpha In Prostate Cancer Progression, Prasanna Ekambaram

Wayne State University Dissertations

Thromboxane A2 (TXA2) is a major arachidonic acid metabolite that signals through TXA2 receptors (TP) to induce platelet aggregation and smooth muscle contraction. TXA2 receptors are expressed as two different isoforms in humans, namely TP-alpha (TPα) and TP-beta (TPβ), which have common and distinct signaling pathways. Of the two TP receptor isoforms, studies have shown that TPα impacts tumor growth and progression of lung cancer. Previously our studies demonstrated that activation of Thromboxane receptor by TXA2 agonists could regulate prostate cancer (PCa) cell motility and cytoskeletal reorganization through activation of Rho-A. The primary objective of this study is to investigate …

Membrane-Bound Immunomodulators As Adjuvants In A Cell Culture-Based Avian Influenza Vaccine, David Daniel Fischer

Wayne State University Dissertations

Inactivated viral vaccines often generate suboptimal immune responses. Adjuvants are incorporated into vaccines to increase their immunogenicity, however currently available adjuvants have shortcomings which have limited their use in human and veterinary medicine. This necessitates the development of new adjuvants and delivery systems. Cytokines have been extensively tested as adjuvants in vaccines but challenges such as diffusion from antigen, short half-lives and production costs have been encountered. To address this, we developed a technology that efficiently produces inactivated, whole-virus influenza vaccine bearing membrane-bound cytokines. Tethering the cytokine to the antigen of interest keeps the immunomodulator in close contact with the …

Corporeal Modification In The Hollywood Musical: New Paradigms For Genre Analysis, Christopher Timothy Gullen

Wayne State University Dissertations

This project explores the trend of bodily and identity modification in the contemporary Hollywood musical and calls for a re-reading of the genre away from the standard classification, that of one strictly imbued with wistful nostalgia and heteronormativity. This work argues that several films have abrogated these traditional ideals to create a carnivalesque representation of the societal norms through a genre, and a production code that sought to preserve them. Using the work of Russian semiotician Mikhail Bakhtin, this project reveals that these films feature dark and satirical para-realities of murder, debauchery and cannibalism that act as a catharsis to …

Narratives Of Possibility: Adolescent Girls Constructing And Negotiating Front And Back Stage Identities Through Blogs, Kattie Marie Hogan

Wayne State University Dissertations

For young woman, the issue of self-expression is important to consider because there are many contexts where their voices and experiences are discounted, silenced, and suppressed. Venues for self-expression can provide a way for those voices and experiences that have been ignored in classrooms and other social outlets to be recognized. This research study explored the ways four girls between the ages of 16-19 used blogging to construct meaning of their experiences and negotiate their identities through a wide range of literary practices including nonfiction and fiction writing, photographs, self-created drawings, and other materials. Writing in these blogs girls moved …

A Role For Reactive Oxygen Species In Photodynamic Therapy, Michael Price

Wayne State University Dissertations

Photodynamic therapy (PDT) is based on the ability of certain photosensitizing agents to selectively localize in neoplastic cells and their vasculature. Subsequent irradiation at a wavelength corresponding to a photosensitizer absorbance band excites the photosensitizer molecules, leading to energy transfer reactions and fluorescence. It was initially concluded that the phototoxic effect occurred when energy from the excited state of the photosensitizer was transferred to dissolved oxygen to form singlet oxygen. This product has a very brief half-life and will cause cellular damage only in the immediate vicinity of its formation. But an excited-state photosensitizer can also interact with oxygen to …

Predicting Vision Loss In Healthy Aging With Manganese-Enhanced Mri Of The Rat Eye, David Bissig

Wayne State University Dissertations

In healthy aging, visual function declines throughout adulthood. Age-related changes in neuronal ion homeostasis -- specifically, increased Ca2+ influx through L-type voltage gated calcium channels (L-VGCCs) -- are believed to contribute to certain functional declines, but this possibility has not yet been tested in the neural retina. In young, mid- and old adult Long-Evans rats, we compared visual function (optokinetic tracking), as well as retinal physiology and eye morphology (Mn2+-enhanced MRI (MEMRI), which uses neuronal Mn2+ uptake as a marker of Ca2+ influx). We documented significant age-related decreases in visual performance and increases in retinal ion influx. We confirmed that …

Substance P And The Host Immune Response, Megan Elizabeth Foldenauer

Wayne State University Dissertations

This body of work examined the mechanism by which SP regulates host immunity, specifically, its control of growth factors and TLR expression in the P. aeruginosa-infected cornea. The role of mTOR and VIP in corneal infection and inflammation also was tested.

SP has a dual role in bacterial infection, unexpectedly upregulating growth factor production. This was accompanied by macrophage-specific upregulation of pro-inflammatory cytokines, downregulation of anti-inflammatory cytokines, and upregulation of anti-apoptotic genes, as well as a decrease in arginase-producing macrophages (M2 cells), important in stromal healing in these mice. All of these lead to worsened disease, despite the stimulatory effects …

Investigation Of Posttranscriptional Regulation After Global Brain Ischemia And Reperfusion Injury, Jeffrey J. Szymanski

Wayne State University Dissertations

The final cause of death in most patients revived after cardiac arrest is ischemia and reperfusion (I/R) injury in the brain. Survival after brain I/R injury depends on the expression of new stress response proteins such as heat shock protein 70 (HSP70). Little is known about why recovering neurons are able to express new stress response proteins while neurons that will die can transcribe RNA but do not translated protein in early reperfusion. Previous studies suggested that the mRNA-binding protein HuR may regulate hsp70 mRNA in reperfused neurons through a novel cytoplasmic structure, the mRNA granule. To determine the roles …

The Effect Of Biodiesel Blends On A Multi-Cylinder Engine And The Impact On Aftertreatment, Amy Peterson

Wayne State University Dissertations

Recently, topics including global warming, increased environmental awareness, recycling, and organically produced meat and vegetables have been prevalent in the media and research arenas. The rapidly increasing cost for fossil fuels and the pursuit of environmental conservation has brought about the interest in a renewable and clean burning fuel, specifically biodiesel. Biodiesel, or vegetable oil ester as it was originally referred to, has been improving and progressing since the early 1900's. Fortunately, significant advancement in the understanding of raw vegetable oil as a fuel as well as converting it to ethyl or methyl esters, now known as biodiesel, has been …

Conflicts As Inner Trials: Transitions For Clients, Ideas For Lawyers, Jonathan R. Cohen

Cardozo Journal of Conflict Resolution

As times of transition, conflicts often produce significant inner trials for parties. This paper categorizes some of the more common inner trials parties in conflict face (e.g., coping with loss, strong emotions, uncertainty, etc.) and suggests that, as liminal times in people's lives, some conflicts may also hold within them important opportunities for learning, growth and self-definition. This paper also offers some ideas for how lawyers might best assist clients during such transitions.

"There Is A World Elsewhere": Preliminary Studies On Alternatives To Interest-Based Bargaining, F. Peter Phillips

Cardozo Journal of Conflict Resolution

We live in an Age of Reason, which our predecessors were pleased to call an Age of Enlightenment. The laws of physics and molecular chemistry, not the appearance of angels to shepherds, define our social expectations. In economics and in politics, we accept the guidance of a collective rationale, an Invisible Hand that, if it doesn't predict, nevertheless records the aggregate of decisions made by millions of social actors, each expressing not the general welfare but their individual self-interest.

Beyond Foreign Policy: A Fresh Look At Cross-Cultural Negotiations And Dispute Resolution Based On The India-United States Nuclear Test Ban Negotiations, Sukhsimranjit Singh

Cardozo Journal of Conflict Resolution

This paper has three goals: first, to study the negotiation of CTBT from Indian and U.S. perspectives; second, to analyze the influence of culture on the processes and outcomes of nuclear negotiations; and third, to provide an approach that would have produced a better result in the CTBT negotiations, and which could be used with more success in future bilateral and multi-lateral negotiations. The approach and lessons from my analysis can also be applied towards other cross-cultural disputes, especially to those that involve national culture. I conclude that the CTBT negotiation between India and the U.S. (among other nations) was …

Morphine And Clonidine Synergize To Ameliorate Low Back Pain In Mice, Maral Tajerian, Magali Millecamps, Laura S. Stone

Publications and Research

Chronic low back pain (LBP) is a debilitating condition associated with signs of axial and radiating pain. In humans with chronic LBP, opioids are often prescribed with varying outcomes and a multitude of side effects. Combination therapies, in which multiple pharmacological agents synergize to ameliorate pain without similar potentiation of adverse reactions, may be useful in improving therapeutic outcome in these patients. The SPARC-null mouse model of low back pain due to disc degeneration was used to assess the effects of opioid (morphine) andα2-adrenergic agonist (clonidine) coadministration on measures of axial and radiating pain. The results indicate that systemic morphine …

Sci-Tech Book News Reviews, Susan Fingerman

Sci-Tech News

No abstract provided.

Association Of Wild Bird Density And Farm Management Factors With The Prevalence Of E. Coli O157 In Dairy Herds In Ohio (2007–2009), N. Cernicchiaro, D. L. Pearl, S. A. Mcewen, L. Harpster, H. J. Homan, G. M. Linz, J. T. Lejeune

United States Department of Agriculture Wildlife Services: Staff Publications

Our objective was to determine the role that European starlings (Sturnus vulgaris) play in the epidemiology of Escherichia coli O157:H7 in dairy cattle. We visited 150 dairy farms in Ohio twice during summer and fall months from 2007 to 2009. Fresh faecal pats from 30 lactating cows were collected during each visit. Information on farm management and environmental variables was gathered through a questionnaire administered to the farm owner. The number of starlings observed on the farm was also recorded. Approximately 1% of dairy cattle and 24% of farms were positive for E. coli O157. Risk factors associated …

Double-Crested Cormorant Distribution On Catfish Aquaculture In The Yazoo River Basin Of Mississippi, Brian S. Dorr, Loren W. Burger, Scott C. Barras, Kristina Casscles Godwin

United States Department of Agriculture Wildlife Services: Staff Publications

Estimating the catfish aquaculture production losses that can be attributed to double-crested cormorants (Phalacrocorax auritus) has proved problematic because knowledge of the distribution of cormorants on catfish aquaculture is lacking. We evaluated use versus availability of various production pond types and landscape-scale factors affecting the distribution of cormorants on channel catfish (Ictalurus punctatus) aquaculture facilities in Mississippi, USA. Cormorant distribution on aquaculture pond types indicated selection against brood-fish ponds, neutral selection on fingerling ponds, and selection for food-fish ponds (n = 29, Chesson’s α = 0.19, 0.36, and 0.45, respectively). Modeled and validated correct classification rate …

Scavenger Community Response To The Removal Of A Dominant Scavenger, Z. H. Olson, J. C. Beasley, T. L. Devault, O. E. Rhodes Jr.

United States Department of Agriculture Wildlife Services: Staff Publications

The alteration of scavenging communities can reduce basic ecosystem services and increase risks to human and wildlife health. Recent work demonstrated that scavenging communities in agricultural landscapes are extremely efficient: superabundant mesopredators sequestered system energy by dominating scavenging activity. To explore how the disturbance of these communities affects the stability of carrion removal as an ecosystem function, we experimentally manipulated a scavenging community within an agricultural landscape by reducing the abundance of the dominant scavenger, raccoons Procyon lotor. We then monitored the fates of 676 mouse Mus musculus carcasses placed in 13 control and 13 removal woodlots from June …

A Novel Isoflurane Anesthesia Induction System For Raccoons, Kevin T. Bentler, Daniel N. Gossett, J. Jeffrey Root

United States Department of Agriculture Wildlife Services: Staff Publications

We developed a novel small-volume (24-L) conical-shaped isoflurane anesthesia induction chamber for use in a den chamber and tested it along with 3 conventional stand-alone induction chambers (2 clear acrylic plastic chambers and a cylindrical-shaped chamber) to determine utility for daily short-duration manipulations of captive raccoons (Procyon lotor). Although the conventional chambers were valuable, the majority of inductions were performed using the cone chamber in a pen setting. With the novel device, we were able to minimize the need for pre-anesthetic handling of animals and eliminate the need for injectable anesthesia agents. As a result, side effects normally …