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An Efficient Method For Stable Transfection Of Mouse Myogenic C2c12 Cell Line Using A Nonviral Transfection Approach, Mehmet Deni̇z Akyüz, Burcu Hayta, Pervi̇n Ruki̇ye Di̇nçer

Turkish Journal of Medical Sciences

To describe in detail the use of the T-REx^{TM} System (a tetracycline-regulated mammalian expression system; Invitrogen) for rapid, easy, and effective generation of stable C2C12 cell clones. Tetracycline-inducible stable cell lines are useful tools to study the function of various genes in different types of mammalian cells. However, the generation of stable cell lines is a time-consuming, technically difficult, and expensive process. Materials and methods: Generation of a stable C2C12 cell line was performed by stable transfection of the cells with the pcDNA6/TR plasmid vector, which contains the tetracycline repressor and the blasticidin drug resistance genes. The establishment of the …

Shale Gas Rock Characterization And 3d Submicron Pore Network Reconstruction, Malek Elgmati

Masters Theses

"Determining shale gas petrophysical properties is the cornerstone to any reservoir-management practice. Hitherto, conventional core analyses are inadequate to attain the petrophysical properties of shale gas at submicron-scale. This study combines interdisciplinary techniques from material science, petrophysics, and geochemistry to characterize different shale gas samples from North America including Utica, Haynesville and Fayetteville shale gas plays. Submicron pore structure, clay mineralogy, wettability and organic matter maturation were investigated to evaluate the petrophysical properties of shale gas rocks and to determine the impact of organic and inorganic matters on wettability alteration for different fracturing fluids on shale gas rocks.

High pressure …

Inherent And Model-Form Uncertainty Analysis For Cfd Simulation Of Synthetic Jet Actuators, Daoru Frank Han

Masters Theses

"A mixed aleatory (inherent) and epistemic (model-form) uncertainty quantification (UQ) analysis method was applied to a computational fluid dynamics (CFD) modeling problem of synthetic jet actuators. A test case, (Case 3, flow over a hump model with synthetic jet actuator control) from the CFDVAL2004 workshop was selected to apply the Second-Order Probability framework implemented with a stochastic response surface obtained from Quadrature-Based Non-Intrusive Polynomial Chaos (NIPC). Three uncertainty sources were considered: (1) epistemic uncertainty in turbulence model, (2) aleatory uncertainty in free stream velocity and (3) aleatory uncertainty in actuation frequency. Uncertainties in both long-time averaged and phase averaged quantities …

Design And Development Of Manufacturing Methods For Manufacturing Of Pem Fuel Cell Mea's, Nikhil Pramod Kulkarni

Masters Theses

"This thesis focuses on design and manufacturing of Fuel cell components using Additive Manufacturing techniques and then in the later part on design and manufacturing of Membrane Electrode Assembly (MEA) which is a very important component in fuel cells. Additive manufacturing methods are fast and efficient manufacturing methods which are additive building up components layer by layer instead of conventional subtractive manufacturing techniques. This ensures low cost and faster manufacturing. Additive manufacturing is important for fuel cell component manufacturing since it is important in fuel cells to minimize wastage and reduce the cost. MEA is the basis of the cost …

The Feasibility Of Using Bifidobacteria Bifidum (Atcc 700541) For The Production Of Prebiotic Oligosaccharides, Candice Marie Luehrs

Masters Theses

"Galactooligosaccharides (GOS) are prebiotics that are being used to influence intestinal microbiota towards health promoting, beneficial bacteria like bifidobacteria. Natural sources of GOS are not found in suitable levels to achieve the desired health effects so economically viable commercial production methods are important. Enzymatic conversion of lactose to GOS through transgalactosylation is a preferred method since the starting material is inexpensive and readily available. Enzymes used in batch production are purified from fungal or bacterial sources or remain in whole cells cultured for GOS production. Purified or unbound enzymes are single use whereas enzymes retained within whole cells can potentially …

Design Of A Hydrogen Community For Santa Monica, Vijay Mohan

Masters Theses

"Hydrogen systems infrastructure development and fuel cell vehicles (FCVs) have the potential to replace the gasoline internal combustion engine vehicles thus increasing energy security and reducing greenhouse gas emissions. Hydrogen fueling infrastructure development is critical for the commercialization and consumer acceptability of the FCVs, thus recognizing hydrogen as an affordable transportation fuel. This paper presents the conceptual design of a scalable and reproducible hydrogen fueling station in Santa Monica, California. The various hydrogen production technologies have been evaluated and the usage of renewable energy sources such as biogas and landfill gas has been emphasized. The technical specifications of the hydrogen …

The Effects Select Physical Parameters Have On An Explosively Formed Projectile's Performance, Phillip R. Mulligan

Masters Theses

"Identifying how changes in the physical parameters of an Explosively Formed Projectile (EFP) affect its performance is crucial in determining what physical parameters could be changed to achieve a desired performance. This research analyzes five of the physical parameters of an EFP, similar to an Iranian design (Worsey, 2009), and the effects on performance. The five physical parameters selected for this research were charge weight, confining geometry, flyer thickness, flyer curvature, and explosives-type. Eighteen different EFP designs were used to test the penetration, measured velocity, production of a dominant projectile, and kinetic energy of these five physical parameters. Of the …

Waterjet Injection Of Powdered Activated Carbon For Sediment Remediation, Chris J. Redell

Masters Theses

"In situ sediment remediation through waterjet-activated carbon amendment delivery is an innovative means to mitigate the dangers posed by hydrophobic organic compounds. Ease of use and applicability to inundated environments makes this technique valuable. This work made feasible low-pressure injections of a 15% (by dry weight) carbon/water slurry through a pulsating piston pump waterjet. The project fabricated nozzle tips and a unique injection apparatus comprising a quantitative system for the evaluation of bench scale models. Based on a series of iterative processes, injections of carbon were analyzed for depth and concentration. Injections varied in duration and they were made into …

Driven Pile Load Test Data For Load And Resistance Factor Design In Missouri, Joseph Ronson Cravens

Masters Theses

"The Missouri Department of Transportation (MoDOT) has recently migrated from allowable stress design (ASD) to load and resistance factor design (LRFD) of driven piles. This transition was initiated when the Federal Highway Administration (FHWA) issued a policy stating that all new bridge designs shall be designed in accordance with the American Association of State Highway and Transportation Officials (AASHTO) LRFD Bridge Design Specifications to eliminate the difference in design methodologies for bridge superstructures and bridge substructures. However, the resistance factors for driven piles specified in the AASHTO LRFD specifications are based on nationwide pile data, consisting of a wide range …

Magnetometer-Only Attitude Determination With Application To The M-Sat Mission, Jason D. Searcy

Masters Theses

"The topic of this thesis focuses on attitude determination for small satellites. The method described uses only a magnetometer to resolve the three-axis attitude of the satellite. The primary challenge is that magnetometers only instantaneously resolve two axes of a satellite's attitude. Typically, magnetometers are used in conjunction with other sensors to resolve all three axes. However, by using a filter over an adequately long orbit arc, the magnetometer data can yield all the information necessary. The magnetic field data are filtered to obtain the magnetic field derivative vector, which are combined with the magnetic field vector to fully resolve …

Educational Robotics: Using The Lego Mindstorms Nxt Platform For Increasing High School Stem Education, Matthew Aaron Strautmann

Masters Theses

"The field of educational robotics (ER) seeks to use the building and programming of robots to engage and educate the next generation of college freshman entering science and engineering majors. To increase the rate of application to science and engineering degree programs as well as the rate of retention, students must be engaged in high school. They must acquire the knowledge and interest to pursue these career choices. This research explores the use of robotics to interest high school students in science, technology, engineering, and math (STEM) and to improve their knowledge of these subjects. The case study developed instructional …

Evaluation Of The Orientation Of 90⁰ And 180⁰ Reinforcing Bar Hooks, Nichole Lynn Podhorsky

Masters Theses

"This thesis describes test results of a study initiated to evaluate the potential influence of hook tilt angle of standard reinforcing hooks on the bond strength of concrete. The topic of the evaluation of the orientation of 90 and 180 degree reinforcing bar hooks in concrete members was identified by the Concrete Reinforcing Steel Institute (CRSI) as high-priority for the reinforcing steel industry. In this test program, a series of single bar and multiple bar specimens were designed and tested to examine bar behavior and potential group effects that may exist in wide flexural members with multiple bars, such as …

Trait Approach To Leadership In Campus Organizations: The Hogan Personality Inventory As A Predictor, Christyn Nicolle Collum

Masters Theses

"The trait approach to leadership has been researched extensively and applied to several fields. This study examined whether or not student leadership in registered student organizations is related to personality traits on the Missouri University of Science and Technology campus from spring semesters 2008, 2009, and 2010. It also explored whether or not traits are related to students joining organizations, during these same semesters. The students' personalities were evaluated using the Hogan Personality Inventory (HPJ), which entering freshmen voluntarily completed. The seven scales of the HPJ are Adjustment, Ambition, Sociability, Interpersonal Sensitivity, Prudence, Inquisitiveness, and Learning Approach. Personality results were …

Characterizing Formation Dredgeability For Clamshell Dredge, Bismark Osei

Masters Theses

"The lack of metrics to assess clamshell dredge formation dredgeability limits the ability of engineers to predict the dredgeability of compact material and increases the risks associated with clamshell selection. Research is, therefore, necessary to increase our understanding of clamshell dredgeability assessment by evaluating possible metrics that will allow the operator to select the optimal clamshell for the operation. Work done and motor energy are possible dredgeability metrics that can be estimated using micro-processor based machine monitoring. The objective of this work is to test the hypotheses that: (i) work done by the pistons during dredging, by a hydraulically actuated …

Risk-Sensitive Preventive Maintenance Policies Using Semivariance, Venkata Manojramam Tirumalasetty

Masters Theses

"Preventive maintenance is an important tool that increases the reliability of the production system by reducing downtime due to failures. In the literature, maintenance and replacement policies for productions systems have been widely studied and modeled. Traditionally preventive maintenance has focused only on minimizing expected costs without considering variability in costs. Cost variability is also commonly known as risk. In a 2003 paper, Chen and Jin used variance of costs as a measure of risk for formulating preventive maintenance policies. The variance criterion that they used ignored the probability of costs exceeding monthly or yearly budgets provided to managers. The …

Development Of Hybrid Composite Bipolar Plates For Proton Exchange Membrane Fuel Cells, Nathaniel James Richie

Masters Theses

"Bipolar plates are one of the most expensive components of a PEM fuel cell and by far the heaviest. Bipolar plates are responsible for providing flow fields for reaction gases, acting as current collectors for electrons liberated during the chemical reaction inside the cell, and providing structural support for the fuel cell stack. Current PEM fuel cell bipolar plate technology is built on the use of sintered graphite which is costly and requires time-consuming machining. Furthermore, due to the brittle nature of graphite, plates must be made relatively thick which adds significant weight and volume to larger stacks, such as …

Experimental Investigation Of Cutting Forces, Temperature And Surface Roughness In End-Milling Of Hastelloy C-2000, Ryan Matthews

Masters Theses

"This thesis presents the results of experimental investigation of the effects of cutting parameters (spindle speed, feed per tooth, axial depth of cut and radial depth of cut) on the three components of cutting forces (F_x, F_y, F_z), workpiece temperature and surface roughness in end-milling of Hastelloy C-2000 alloy using design of experiments (DOE) and response surface methodology (RSM). End-milling experiments were conducted using 0.5 inch diameter end-mill on Cincinnati Milacron Sabre 750 vertical machining center using water based flood coolant. An inscribed central composite design of experiment was used to study the four …

Development Of A Graphical User Interface For The Study Of Parallel-Connected Solar Arrays, Nisha Nagarajan

Masters Theses

"For any solar array it is necessary to be able to harness the maximum possible power from it. A new technology is proposed to improve the efficiency of solar arrays and harness maximum potential power as well by connecting the solar panels in parallel. This thesis describes the development of a graphical user interface for the evaluation of this parallel-connected solar array technology and to compare different scenarios for solar arrays to predict the potential power output to decide the best configuration for an application. This is done via several computations and analysis of field data. The first paper describes …

Economic Evaluation Of Sustainable Strategies For The Transportation Sector, Yaqin Lin

Masters Theses

"Sustainable strategies are drawing growing attention as global environmental challenges increase concerns over energy consumption. As one of the most intensive consumers of energy, the transportation sector has a particular need to go green. Implementing alternative energy sources and applying advanced energy efficient technologies are two major approaches to achieve sustainability. Much work thus far used cost-effectiveness as an important criterion for assessing the practicability of sustainable strategies. However, few studies have evaluated such strategies systematically. This thesis has developed a framework of cost-effectiveness analysis to assess sustainability strategies. The framework summarizes the various effects of five representative alternative energy …

Fabrication Of Sers Active Nanostructures By Femtosecond Laser Micromachining, Matthew Aaron Olson

Masters Theses

"This work investigated the surface-enhanced Raman scattering (SERS) enhancement of gold nanoparticles on femtosecond laser created nanostructures on a silicon wafer. The goal was to use the nanostructures to assemble the nanoparticles into three-dimensional aggregates and compare these enhancements to those of two dimensional aggregates. The SERS enhancement generated by the three-dimensional structures was on average 5 x 10⁴ with a high enhancement of 1.2 x 10⁵. The magnitude of this enhancement was on par with previous experiments that tested SERS enhancements on gold nanoparticles, but it did not show any significant enhancement caused by the three-dimensional nature of the …

Governmental And Private Liability For Flooding, Michael B. Gerrard

Faculty Scholarship

In recent years the frequency and severity of heavy precipitation and floods in parts of the United States, including the Northeast, have been increasing to a statistically significant degree, and this trend is expected to worsen. Events such as last August’s Hurricane Irene have caused widespread loss of life and property damage.

This article summarizes some of the liability issues that result from floods, and efforts to control them.

Virtual Prototype Modeling And Simulation Of Pipe Wagon Articulating System, Y. Li, Samuel Frimpong, W. Y. Liu

Mining Engineering Faculty Research & Creative Works

Virtual prototype of pipe wagon articulating (PWA) system has been developed and simulated based on the kinematics and dynamics of machinery and Automatic Dynamic Analysis of Mechanical Systems (ADAMS) software. It has been integrated with real-time three dimensional (3-D) system simulations for detailed and responsive interaction with dynamic virtual environments. By using this virtual model, the conceptual design examination and performance analysis of the PWA system have been realized dynamically in virtual laboratory. System dynamic force, displacement and tension of pipe have been measured through verifying this 3- D virtual prototype. By comparing the static tension and dynamic tension of …

Keynote Discussion: Just Exactly What Does A Transactional Lawyer Do?, William J. Carney, Ronald J. Gilson, George W. Dent

Faculty Scholarship

Panel discussion from the Center for Transactional Law and Practice at Emory University School of Law’s second biennial Transactional Law Conference, “Transactional Education: What's Next?” (June 2010).

Model Municipal Ordinance Project Designed To Facilitate Wind And Solar Projects And Green Buildings, Michael B. Gerrard, Danielle Sugarman

Faculty Scholarship

Soaring oil prices and the reality of climate change have underscored the heed to reduce U.S. fossil fuel dependence by improving energy efficiency and by developing and expanding renewable sources of energy. The International Energy Agency declared in 2010 that "[i]ncreasing energy efficiency, much of which can be achieved through low-cost options, offers the greatest potential for reducing CO2 emissions over the period to 2050." Furthermore, increasing our reliance on renewable resources such as wind and solar energy is not only a prudent measure in helping America to improve its energy security, but is a necessary component of a basket …

The Optimization Of Adna Extraction Protocol And Sex Determination Of Bronze Age Individuals From Oylum Höyük (Kilis, Turkey), Serdal Arslan, Ayşen Açikkol, Ertan Mahi̇r Korkmaz

Turkish Journal of Biology

Oylum Höyük falls within the borders of the province of Kilis, located in the southern part of Turkey. It is an important Bronze Age settlement dating back to 5500 BC. In this study, the sex of 36 individuals found in Oylum Höyük was examined using morphological and genetic methods. In the morphological method, sex was identified in 14 female and 13 male individuals. The remaining 8 individuals could not be identified due to the poor preservation of their skeletal remains. Genetic sex was determined with the amplification of sex-specific X, Y centromeric alphoid repeats and ZFX-ZYF gene regions of DNA, …

Detection Of Prokaryotic Microbial Communities Of Çamaltı Saltern, Turkey, By Fluorescein In Situ Hybridization And Real-Time Pcr, Mehmet Burçi̇n Mutlu, Kiymet Güven

Turkish Journal of Biology

Knowledge of the functions, interactions, and diversity of extremely halophilic microorganisms mostly comes from the results of studies performed in different salterns throughout the world. As model habitats, salterns allow the comparison of different techniques used for qualitative and quantitative analysis of halophilic communities in these hypersaline environments. Çamaltı Saltern is the biggest coastal solar saltern located on the Aegean coast of Turkey, and it produces most of the salt consumed in the country. In the present study, detection of prokaryotic communities of the Çamaltı Saltern was performed using 2 culture-independent methods. Real-time polymerase chain reaction (RT-PCR) and fluorescein in …

Production Of A Novel Bifunctional Catalase-Phenol Oxidase Of Scytalidium Thermophilum In The Presence Of Phenolic Compounds, Yonca Yüzügüllü, Zümrüt Begüm Ögel, Ufuk Bölükbaşi, Nursen Çoruh, Gürkan Karakaş

Turkish Journal of Biology

A novel bifunctional catalase with additional phenol oxidase activity (CATPO) is produced by the thermophilic fungus, Scytalidium thermophilum, in a growth-associated manner. In order to study the biological significance of this dual enzyme in relation to phenolic compounds, 14 phenolics were tested for their effect on growth and CATPO production. It was determined that some phenolics exerted a negative effect on growth (catechol, coumaric acid, hydroquinone, kaempferol, myricetin), while others either did not influence growth in a negative manner or enhanced growth by a maximum of 50% increase in biomass (caffeic acid, chlorogenic acid, catechin, gallic acid, resorcinol, vanillic acid). …

Cover And Contents

Turkish Journal of Biology

No abstract provided.

Cover And Contents

Turkish Journal of Biology

No abstract provided.

Friction Stir Lap Welded Advanced High Strength Steels: Microstructure And Mechanical Properties, M. Ghosh, K. Kumar, R. S. Mishra

Materials Science and Engineering Faculty Research & Creative Works

Friction stir welding was carried out under different heat input and cooling rates to produce lap joints between high strength martensitic steel sheets. the microstructure of the welds was characterized, and microhardness was evaluated. Joint efficiency was determined by lap shear test. Variation in processing conditions governed total heat input, peak temperature and cooling rate during friction stir welding. Weld nugget microstructure depended principally on cooling rate. the slowest cooling rate promoted ferrite-pearlite and the fastest cooling rate resulted in martensite formation in the weld nugget. the weakest region of all the joints was the heat affected zone, which consists …